18 results on '"G. Roncarati"'
Search Results
2. Validation of a Duplex real-time PCR assay for the microbiological diagnosis of Rickettsia spp. infection in the metropolitan area of Bologna
- Author
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E. C. Borgatti, G. Virgili, G. Roncarati, I. Bon, L. Attard, P. Viale, A. Marangoni, T. Lazzarotto, and S. Galli
- Subjects
Microbiology ,QR1-502 - Abstract
Not available.
- Published
- 2024
- Full Text
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3. Spread of Toxoplasma gondii among animals and humans in Northern Italy: A retrospective analysis in a One-Health framework
- Author
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F.M. Dini, S. Morselli, A. Marangoni, R. Taddei, G. Maioli, G. Roncarati, A. Balboni, F. Dondi, F. Lunetta, and R. Galuppi
- Subjects
Toxoplasmosis ,Epidemiology ,Humans ,Animals ,Emilia-Romagna region ,Serology ,Infectious and parasitic diseases ,RC109-216 - Abstract
Toxoplasmosis occurs worldwide and is considered one of the most important food-borne parasitic zoonoses. The consumption of undercooked meat containing viable tissue cysts and ingestion of environmental oocyst are the most important sources of infection. The aim of this retrospective study was to evaluate the spread of Toxoplasma gondii in the province of Bologna (Emilia-Romagna region) in northern Italy, with a One Health approach, comparing seropositivity rates in different animal species and in humans over the last 19 and 4 years respectively. Analyses were performed on serological data collected over different periods at three separate locations: Istituto Zooprofilattico Sperimentale della Lombardia e della Emilia-Romagna (IZSLER); Veterinary University Hospital Clinical Pathology Service, Department of Veterinary Medical Sciences, University of Bologna; and Unit of Microbiology, St. Orsola Hospital, Bologna. Most relevant seropositivity rates observed in animals were 15.5% (wild boar), 25% (roe deer), 18.7% (goat), 29.9% (sheep), 9.7% (pigs), 42.9% and 21.8% in cat and dog, respectively. A comprehensive screening was conducted on a population of 36,814 individuals, revealing a prevalence of 20.4%. Among pregnant women, a frequence of 0.39% for active toxoplasmosis was observed. Despite certain limitations, this study provided valuable insights into the extensive distribution of this parasitic infection among diverse animal species and human populations in the province of Bologna. These findings underscore the importance of implementing consistent and proactive toxoplasmosis screening protocols during pregnancy, while emphasizing the critical need for adopting a One Health approach for effective control of this parasitic disease.
- Published
- 2023
- Full Text
- View/download PDF
4. Le infezioni da Clostridium difficile nel Presidio Ospedaliero Unico dell’Azienda USL di Bologna: incidenza e fattori di rischio
- Author
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G. Roncarati, E. Panico, P. Farruggia, DALLOLIO, LAURA, LEONI, ERICA, SIMPIOS, G. Roncarati, E. Panico, L. Dallolio, E. Leoni, and P. Farruggia
- Subjects
Clostridium difficile ,infezioni ospedaliere - Published
- 2012
5. Preparation and microstructural characterization of nanosized Mo-TiO2 and Mo-W-O thin films by sputtering: tailoring of composition and porosity by thermal treatment
- Author
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Giorgio Sberveglieri, G. Della Mea, Elisabetta Comini, G. Roncarati, Matteo Ferroni, Vincenzo Guidi, Alberto Vomiero, and Giuliano Martinelli
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Materials science ,Annealing (metallurgy) ,Mechanical Engineering ,Doping ,Metallurgy ,Oxide ,Thermal treatment ,Condensed Matter Physics ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,Mechanics of Materials ,Sputtering ,General Materials Science ,Thin film ,Porosity ,Inert gas - Abstract
Nanosized Mo doped TiO 2 and mixed W–Mo oxide thin films were deposited via RF-magnetron sputtering, in either reactive or inert atmosphere, to study the influence of the plasma gas on the film growth. Mixed targets (Ti (97 at.%)–Mo (3 at.%) and W (12 at.%)–Mo (88 at.%)) were used for the purpose. Thermal treatments were performed to promote structural and chemical changes. Experimental evidences highlighted the dependence of structural, morphological and compositional evolution of the films on the annealing temperature. In particular, coalescence inhibition to titania nanograins induced by Mo was observed in the Mo doped TiO 2 films. The different volatility of WO 3 and MoO 3 in the W–Mo mixed oxides greatly affected the evolution of the film through annealing. This method proved useful to tailor the composition and porosity of the layers. Mo doped TiO 2 films were tested as chemoresistive sensors towards ethanol; mixed W–Mo oxide were found to respond to CO.
- Published
- 2003
6. 5-MTHF enhances the portal pressure reduction achieved with propranolol in patients with cirrhosis: A randomized placebo-controlled trial.
- Author
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Vukotic R, Di Donato R, Roncarati G, Simoni P, Renzulli M, Gitto S, Schepis F, Villa E, Berzigotti A, Bosch J, and Andreone P
- Subjects
- Humans, Propranolol therapeutic use, Propranolol pharmacology, Nitric Oxide Synthase Type III pharmacology, Nitric Oxide Synthase Type III therapeutic use, Portal Pressure, Nitric Oxide, Gastrointestinal Hemorrhage prevention & control, Adrenergic beta-Antagonists therapeutic use, Adrenergic beta-Antagonists pharmacology, Liver Cirrhosis complications, Liver Cirrhosis drug therapy, Esophageal and Gastric Varices complications, Hypertension, Portal etiology, Hypertension, Portal complications
- Abstract
Background & Aims: β-blockers reduce hepatic venous pressure gradient (HVPG) by decreasing portal inflow, with no reduction in intrahepatic vascular resistance. 5-Methyltetrahydrofolate (5-MTHF) can prevent oxidative loss of tetrahydrobiopterin (BH4), a cofactor for endothelial nitric oxide synthase coupling. It also converts homocysteine (tHcy) into methionine and enables the degradation of asymmetric dimethylarginine (ADMA), an inhibitor of endothelial nitric oxide synthase. The aim of this study was to evaluate the effects of 5-MTHF in combination with propranolol on HVPG and nitric oxide bioavailability markers in patients with cirrhosis and portal hypertension., Method: Sixty patients with cirrhosis and HVPG ≥12 mmHg were randomized 1:1 to receive treatment with 5-MTHF+propranolol or placebo+propranolol for 90 days under double-blind conditions. HVPG and markers of nitric oxide bioavailability (BH4, ADMA and tHcy) were measured again at the end of treatment., Results: Groups were similar in terms of baseline clinical and hemodynamic data and nitric oxide bioavailability markers. HVPG decreased in both groups, but the magnitude of the change was significantly greater in the group treated with 5-MTHF+propranolol compared to placebo+propranolol (percentage decrease, 20 [29-9] vs. 12.5 [22-0], p = 0.028), without differences in hepatic blood flow. At the end of treatment, 5-MTHF+propranolol (vs. placebo+propranolol) was associated with higher BH4 (1,101.4 ± 1,413.3 vs. 517.1 ± 242.8 pg/ml, p <0.001), lower ADMA (109.3 ± 52.7 vs. 139.9 ± 46.7 μmol/L, p = 0.027) and lower tHcy (μmol/L, 11.0 ± 4.6 vs. 15.4 ± 7.2 μmol/L, p = 0.010) plasma levels., Conclusion: In patients with cirrhosis and portal hypertension, 5-MTHF administration significantly enhanced the HVPG reduction achieved with propranolol. This effect appears to be mediated by improved nitric oxide bioavailability in the hepatic microcirculation., Clinical Trial Eudract Number: 2014-002018-21., Impact and Implications: Currently, the pharmacological prevention of cirrhosis complications due to portal hypertension, such as esophageal varices rupture, is based on the use of β-blockers, but some patients still present with acute variceal bleeding, mainly due to an insufficient reduction of portal pressure. In this study, we sought to demonstrate that the addition of folic acid to β-blockers is more effective in reducing portal pressure than β-blockers alone. This finding could represent the basis for validation studies in larger cohorts, which could impact the future prophylactic management of variceal bleeding in cirrhosis. Enhancing the benefit of β-blockers with a safe, accessible, cost-effective drug could improve clinical outcomes in cirrhosis, which in turn could translate into a reduction in the rates and costs of hospitalization, and ultimately into improved survival., (Copyright © 2023 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
7. Spread of Toxoplasma gondii among animals and humans in Northern Italy: A retrospective analysis in a One-Health framework.
- Author
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Dini FM, Morselli S, Marangoni A, Taddei R, Maioli G, Roncarati G, Balboni A, Dondi F, Lunetta F, and Galuppi R
- Abstract
Toxoplasmosis occurs worldwide and is considered one of the most important food-borne parasitic zoonoses. The consumption of undercooked meat containing viable tissue cysts and ingestion of environmental oocyst are the most important sources of infection. The aim of this retrospective study was to evaluate the spread of Toxoplasma gondii in the province of Bologna (Emilia-Romagna region) in northern Italy, with a One Health approach, comparing seropositivity rates in different animal species and in humans over the last 19 and 4 years respectively. Analyses were performed on serological data collected over different periods at three separate locations: Istituto Zooprofilattico Sperimentale della Lombardia e della Emilia-Romagna (IZSLER); Veterinary University Hospital Clinical Pathology Service, Department of Veterinary Medical Sciences, University of Bologna; and Unit of Microbiology, St. Orsola Hospital, Bologna. Most relevant seropositivity rates observed in animals were 15.5% (wild boar), 25% (roe deer), 18.7% (goat), 29.9% (sheep), 9.7% (pigs), 42.9% and 21.8% in cat and dog, respectively. A comprehensive screening was conducted on a population of 36,814 individuals, revealing a prevalence of 20.4%. Among pregnant women, a frequence of 0.39% for active toxoplasmosis was observed. Despite certain limitations, this study provided valuable insights into the extensive distribution of this parasitic infection among diverse animal species and human populations in the province of Bologna. These findings underscore the importance of implementing consistent and proactive toxoplasmosis screening protocols during pregnancy, while emphasizing the critical need for adopting a One Health approach for effective control of this parasitic disease., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)
- Published
- 2023
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8. Environmental Contamination by SARS-CoV-2 During Noninvasive Ventilation in COVID-19.
- Author
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Dell'Olio A, Vocale C, Primavera A, Pisani L, Altavilla S, Roncarati G, Tumietto F, Viale P, Re MC, Lazzarotto T, Nava S, Ranieri VM, and Tonetti T
- Subjects
- Humans, Prospective Studies, Ribonuclease P, SARS-CoV-2, Intensive Care Units, COVID-19, Noninvasive Ventilation, Equipment Contamination
- Abstract
Background: Environmental contamination by SARS-CoV-2 from patients with COVID-19 undergoing noninvasive ventilation (NIV) in the ICU is still under investigation. This study set out to investigate the presence of SARS-CoV-2 on surfaces near subjects receiving NIV in the ICU under controlled conditions (ie, use of dual-limb circuits, filters, adequate room ventilation)., Methods: This was a single-center, prospective, observational study in the ICU of a tertiary teaching hospital. Four surface sampling areas, at increasing distance from subject's face, were identified; and each one was sampled at fixed intervals: 6, 12, and 24 h. The presence of SARS-CoV-2 was detected with real-time reverse transcriptase-polymerase-chain-reaction (RT-PCR) test on environmental swabs; the RT-PCR assay targeted the SARS-CoV-2 virus nucleocapsid N1 and N2 genes and the human RNase P gene as internal control., Results: In a total of 256 collected samples, none were positive for SARS-CoV-2 genetic material, whereas 21 samples (8.2%) tested positive for RNase P, thus demonstrating the presence of genetic material unrelated to SARS-CoV-2., Conclusions: Our data show that application of NIV in an appropriate environment and with correct precautions leads to no sign of surface environmental contamination. Accordingly, our data support the idea that use of NIV in the ICU is safe both for health care workers and for other patients., Competing Interests: The authors have disclosed no conflicts of interest., (Copyright © 2023 by Daedalus Enterprises.)
- Published
- 2023
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9. Evaluation of pregenomic HBV RNA in HBeAg-negative patients.
- Author
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Roncarati G, Galli S, Ferniani T, Moroni A, and Lazzarotto T
- Subjects
- Biomarkers, DNA, Viral genetics, Humans, RNA, Retrospective Studies, Hepatitis B e Antigens, Hepatitis B virus genetics
- Abstract
The distinction between chronic HBeAg-negative hepatitis (CHB) and chronic HBeAg-negative infection (CIB) can be challenging and important for providing advice on prognosis, as well as determining need for treatment. The aim of the present study was to evaluate pgRNA levels in treatment-naïve HBeAg-negative chronic HBV-infected patients. In addition, pgRNA levels were compared to traditional markers in order to assess their clinical utility. A retrospective study was carried out, including 85 cases of CHBs and 74 CIBs. Globally, when the virological markers (pgRNA, qHBsAg, and HBV DNA) were analyzed, significant differences were found between the CHB and CIB groups (P<0.001). Overall, positive correlations were demonstrated, as follows: between pgRNA levels and qHBsAg (Spearman r=0.30, P<0.001), between pgRNA and HBV DNA (Spearman r=0.73, P<0.001), and between pgRNA and ALT (Spearman r=0.67, P<0.001). Out of the 85 CHB patients, 82 (96.5%) agreed to start treatment. At baseline, 38/82 patients, as well as the 3 untreated CHB patients, had undetectable pgRNA levels. The 74 CIB carriers also had undetectable pgRNA levels. During the follow-up period, no patients experienced viral reactivation or progression of liver disease. These results suggest that the addition of plasmatic HBV-pgRNA levels to the traditional diagnostic flowchart of HBeAg-negative patients may improve the correct identification of cases at risk, especially patients with occasional increases in HBV viremia.
- Published
- 2022
10. Rapid identification and detection of β-lactamase-producing Enterobacteriaceae from positive blood cultures by MALDI-TOF/MS.
- Author
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Roncarati G, Foschi C, Ambretti S, and Re MC
- Subjects
- Ertapenem, Humans, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, beta-Lactamases, Blood Culture, Enterobacteriaceae
- Abstract
Objectives: Current evidence suggests that early diagnosis of sepsis and timely detection of antimicrobial resistance are crucial to improve mortality rates among patients. The aim of this study was to evaluate a rapid method for the identification of Gram-negative bacteria from positive blood cultures (BCs), combined with the detection of extended spectrum β-lactamases (ESβL) and carbapenemases production, by means of MALDI-TOF/MS analysis., Methods: During the study, all BCs positive for Gram-negative rods were selected. Starting from bacterial pellets obtained directly from BC broths, species identification and hydrolysis assays were achieved through MALDI-TOF/MS (Bruker). In particular, we performed a hydrolysis assays of cefotaxime (CTX) and ertapenem (ERT) for the rapid detection of resistance via ESβL and carbapenemases, respectively. These results were compared with the routine workflow, including BC subcultures and confirmation phenotypic methods. Finally, a comparison of the turnaround-time (TAT) between the two protocols was conducted., Results: Overall, 185 BCs positive for Enterobacteriaceae were collected. In terms of species identification, we observed a concordance of 95.9% comparing MALDI-TOF/MS results to the subculture-based method. The sensitivity and specificity for CTX hydrolysis assay were 91.1% and 92%, respectively; ERT hydrolysis assay showed a sensitivity of 96.2% and a specificity of 99.2%. The TAT of the proposed MALDI TOF/MS-based protocol was significantly lower compared with the routine workflow (P < 0.0001)., Conclusions: The proposed protocol can provide reliable bacterial identification and data concerning β-lactam resistance in only 3 hours, positively improving management of patients in terms of antimicrobial stewardship., (Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2021
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11. Pharyngeal Chlamydia and gonorrhea: a hidden problem.
- Author
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Gaspari V, Marangoni A, D'Antuono A, Roncarati G, Salvo M, Foschi C, and Re MC
- Subjects
- Adult, Chlamydia Infections epidemiology, Chlamydia Infections microbiology, Chlamydia trachomatis genetics, Female, Genotype, Gonorrhea epidemiology, Gonorrhea microbiology, Homosexuality, Male, Humans, Italy epidemiology, Lymphogranuloma Venereum epidemiology, Lymphogranuloma Venereum microbiology, Male, Middle Aged, Neisseria gonorrhoeae genetics, Polymerase Chain Reaction, Prevalence, Chlamydia Infections diagnosis, Chlamydia trachomatis isolation & purification, Gonorrhea diagnosis, Lymphogranuloma Venereum diagnosis, Neisseria gonorrhoeae isolation & purification, Pharynx microbiology, Rectum microbiology
- Published
- 2019
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12. Syphilis in a high-density urban area in the North of Italy.
- Author
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Roncarati G, Fazio C, Gaspari V, Marziali G, Furlini G, Galli S, Moroni A, Foschi C, Marangoni A, and D'Antuono A
- Subjects
- Coinfection, Female, HIV Infections complications, Humans, Italy epidemiology, Male, Pregnancy, Retrospective Studies, Sex Factors, Syphilis complications, Syphilis epidemiology
- Abstract
Although far less common now than in the past, syphilis continues to pose a danger to public health and should not be overlooked. In this study, we evaluated the presence and characteristics of syphilis in a group of patients attending an STI Clinic in the North of Italy. A retrospective study was carried out, analysing the data from the 5609 subjects who attended the STI Clinic of St. Orsola-Malpighi Hospital (Bologna) for syphilis screening from January 2016 to December 2017. Globally, 692 patients (12.3%) were found positive for treponemal tests, with a significant difference between males and females (16.6% vs 4.1%; P<0.0001). Moreover, positive women were more likely foreign (63.3%), in contrast to men, who were more likely Italian (86.1%; P<0.0001). A total of 306 patients (44.2%), mainly males (47% vs 25%; P=0.0003), received a diagnosis of early syphilis. These cases peaked among patients 35-44 years (31%) and 25-34 years (26.8%). Overall, 32.9% of the women found positive for treponemal tests were pregnant. Among them, 84.6% were foreign (mainly from Eastern Europe) and 38.4% received a diagnosis of early syphilis. No cases of mother-to-child syphilis were found. The presence of an HIV-syphilis co-infection was found in 21.5% of patients with early syphilis, with a significant association with the male sex (P<0.009). In-depth knowledge of the characteristics of syphilis could help set up effective strategies for its control.
- Published
- 2019
13. Potential role of T2Candida in the management of empirical antifungal treatment in patients at high risk of candidaemia: a pilot single-centre study.
- Author
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Giannella M, Paolucci M, Roncarati G, Vandi G, Pascale R, Trapani F, Bartoletti M, Tedeschi S, Ambretti S, Lewis R, Re MC, and Viale P
- Subjects
- Humans, Magnetic Resonance Spectroscopy, Predictive Value of Tests, Prospective Studies, Risk Factors, Sensitivity and Specificity, Antifungal Agents therapeutic use, Candidemia diagnosis, Candidemia drug therapy, Diagnostic Tests, Routine methods, Disease Management, Microbiological Techniques methods, Molecular Diagnostic Techniques methods
- Abstract
Objectives: We estimated the diagnostic accuracy of T2Candida, with blood culture (BC) as the gold standard, and compared turnaround time between these two techniques in order to investigate the potential role of T2Candida in the management of empirical antifungal treatment (EAT)., Methods: We performed a single-centre prospective observational study in patients with severe sepsis or septic shock and multiple risk factors for candidaemia., Results: We analysed 46 out of 50 screened patients. All patients received an echinocandin as EAT; the median EAT duration was 7 days (IQR 4-13 days). BCs were negative in 31 (67.4%) patients, positive for bacteria in 14 (30.4%) patients and positive for Candida albicans in 1 (2.2%) patient. T2Candida was negative, invalid and positive in 37, 5 and 4 patients, respectively. T2Candida and BC results were concordant in all but three patients, where T2Candida was positive and BCs were negative. Two of them were on antifungal prophylaxis at the time of enrolment. T2Candida reduced time to a negative result by 5 days. T2Candida performance was: sensitivity = 100% (95% CI 2.5%-100%), specificity = 91.8% (95% CI 78%-98%), positive predictive value = 25% (95% CI 0.63%-80.6%) and negative predictive value = 100% (95% CI 89.7%-100%)., Conclusions: In patients with multiple risk factors for candidaemia and severe sepsis or septic shock, T2Candida may be helpful to reduce the length of EAT.
- Published
- 2018
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14. Five-year surveillance of human tuberculosis caused by Mycobacterium bovis in Bologna, Italy: an underestimated problem.
- Author
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Lombardi G, Botti I, Pacciarini ML, Boniotti MB, Roncarati G, and Dal Monte P
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Female, Humans, Infant, Italy epidemiology, Male, Middle Aged, Minisatellite Repeats, Multilocus Sequence Typing, Mycobacterium bovis genetics, Tuberculosis microbiology, Genotype, Mycobacterium bovis physiology, Tuberculosis epidemiology
- Abstract
Human tuberculosis (TB) caused by Mycobacterium bovis surveillance is affected by a lack of data. The aims of the present study were: (i) to estimate the proportion of human TB caused by M. bovis over a period of 5 years in Bologna, Northern Italy, which, like most Western European countries, has been declared bovine TB-free; (ii) to compare the genetic profiles of M. bovis strains identified in humans with those circulating in cattle in the last 15 years in Italy. Among 511 TB patients, the proportion of human TB caused by M. bovis was 1·76%, significantly associated to extra-pulmonary localization (P = 0·004) and to being elderly (P < 0·001) and Italy-born (P = 0·036). The molecular epidemiology analysis by spoligotyping and Multilocus Variable Tandem Repeat Analysis confirmed that most M. bovis strains from Italy-born patients matched those circulating in cattle herds in Italy between 2001 and 2016. Two cases of Mycobacterium bovis BCG infection were also characterized. In conclusion, the rate of human TB caused by M. bovis was not negligible, highlighting the relevance of molecular typing in evaluating the effectiveness of programmes designed to eradicate TB in cattle in Italy.
- Published
- 2017
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15. Surveillance of Clostridium difficile Infections: Results from a Six-Year Retrospective Study in Nine Hospitals of a North Italian Local Health Authority.
- Author
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Roncarati G, Dallolio L, Leoni E, Panico M, Zanni A, and Farruggia P
- Subjects
- Aged, Aged, 80 and over, Anti-Bacterial Agents therapeutic use, Clostridium Infections prevention & control, Cross Infection prevention & control, Epidemiological Monitoring, Feces microbiology, Female, Hospitalization, Hospitals statistics & numerical data, Humans, Incidence, Infection Control methods, Italy epidemiology, Male, Proton Pump Inhibitors therapeutic use, Retrospective Studies, Risk Factors, Clostridioides difficile isolation & purification, Clostridium Infections epidemiology, Cross Infection epidemiology
- Abstract
Clostridium difficile is an emerging cause of healthcare associated infections. In nine hospitals of an Italian Local Health Authority the episodes of C. difficile infection (CDI) were identified using the data registered by the centralized Laboratory Information System, from 2010 to 2015. CDI incidence (positive patients for A and/or B toxins per patients-days) was analysed per year, hospital, and ward. A number of cases approximately equivalent to the mean of identified cases per year were studied retrospectively to highlight the risk factors associated to CDI and their severity. Nine hundred and forty-two patients affected by CDI were identified. The overall incidence was 3.7/10,000 patients-days, with a stable trend across the six years and the highest rates observed in smaller and outlying hospitals (up to 17.8/10,000), where the admitted patients were older and the wards with the highest incidences (long-term-care: 7.6/10,000, general medicine: 5.7/10,000) were more represented. The mean age of patients in each hospital was correlated with CDI rates. Of the 101 cases selected for the retrospective study, 86.1% were healthcare associated, 10.9% community acquired; 9.1% met the criteria for recurrent case and 23.8% for severe case of CDI. The overall mortality rate was 28.7%. Comorbidity conditions occurred in 91.1%, previous exposure to antibiotics in 76.2%, and proton pump inhibitors in 77.2%. Recurrent and severe cases were significantly associated with renal insufficiency and creatinine levels ≥2 mg/dL. The survey based on the centralized laboratory data was useful to study CDI epidemiology in the different centres in order to identify possible weaknesses and plan control strategies, in particular the reinforcement of staff training, mainly targeted at compliance with contact precautions and hand hygiene., Competing Interests: The authors declare no conflict of interest.
- Published
- 2017
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16. Use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) to detect carbapenemase production in Enterobacteriaceae by a rapid meropenem degradation assay.
- Author
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Foschi C, Franza V, Conti M, Tamburini MV, Roncarati G, Cordovana M, Smirnova V, Patrono D, Mancini R, Landini MP, and Ambretti S
- Subjects
- Anti-Bacterial Agents chemistry, Bacterial Proteins analysis, Bacterial Proteins chemistry, Biocatalysis, Enterobacteriaceae chemistry, Enterobacteriaceae isolation & purification, Enterobacteriaceae metabolism, Humans, Meropenem, Thienamycins chemistry, beta-Lactamases metabolism, Anti-Bacterial Agents metabolism, Bacterial Proteins metabolism, Chromatography, Liquid methods, Enterobacteriaceae enzymology, Enterobacteriaceae Infections microbiology, Tandem Mass Spectrometry methods, Thienamycins metabolism, beta-Lactamases chemistry
- Abstract
We evaluated the analytical performance of a liquid chromatography-tandem mass spectrometry assay to detect carbapenemase activity in a group of carbapenemase-producing Enterobacteriaceae by meropenem hydrolysis. This one-hour method showed a sensitivity of 94% and a specificity of 100%, representing a rapid and reliable option compared to conventional phenotypic assays.
- Published
- 2015
17. Effect of different disinfection protocols on microbial and biofilm contamination of dental unit waterlines in community dental practices.
- Author
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Dallolio L, Scuderi A, Rini MS, Valente S, Farruggia P, Sabattini MA, Pasquinelli G, Acacci A, Roncarati G, and Leoni E
- Subjects
- Biofilms, Community Dentistry, Drinking Water standards, Dental Equipment microbiology, Disinfection methods, Water Microbiology
- Abstract
Output water from dental unit waterlines (DUWLs) may be a potential source of infection for both dental healthcare staff and patients. This study compared the efficacy of different disinfection methods with regard to the water quality and the presence of biofilm in DUWLs. Five dental units operating in a public dental health care setting were selected. The control dental unit had no disinfection system; two were disinfected intermittently with peracetic acid/hydrogen peroxide 0.26% and two underwent continuous disinfection with hydrogen peroxide/silver ions (0.02%) and stabilized chlorine dioxide (0.22%), respectively. After three months of applying the disinfection protocols, continuous disinfection systems were more effective than intermittent systems in reducing the microbial contamination of the water, allowing compliance with the CDC guidelines and the European Council regulatory thresholds for drinking water. P. aeruginosa, Legionella spp, sulphite-reducing Clostridium spores, S. aureus and β-haemolytic streptococci were also absent from units treated with continuous disinfection. The biofilm covering the DUWLs was more extensive, thicker and more friable in the intermittent disinfection dental units than in those with continuous disinfection. Overall, the findings showed that the products used for continuous disinfection of dental unit waterlines showed statistically better results than the intermittent treatment products under the study conditions.
- Published
- 2014
- Full Text
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18. Outbreak of Citrobacter freundii carrying VIM-1 in an Italian Hospital, identified during the carbapenemases screening actions, June 2012.
- Author
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Gaibani P, Ambretti S, Farruggia P, Bua G, Berlingeri A, Tamburini MV, Cordovana M, Guerra L, Mazzetti M, Roncarati G, Tenace C, Moro ML, Gagliotti C, Landini MP, and Sambri V
- Subjects
- Aged, Aged, 80 and over, Anti-Bacterial Agents pharmacology, Citrobacter freundii classification, Citrobacter freundii drug effects, Cross Infection microbiology, Enterobacteriaceae Infections microbiology, Female, Hospitals, Humans, Italy epidemiology, Male, Microbial Sensitivity Tests, Multilocus Sequence Typing, Phylogeny, beta-Lactamases genetics, Citrobacter freundii genetics, Cross Infection epidemiology, Disease Outbreaks, Enterobacteriaceae Infections epidemiology
- Abstract
Objective: The identification of patients colonized or infected with carbapenemase-producing Enterobacteriaceae (CPE), in order to control and prevent the global spread of multidrug-resistant (MDR) pathogens., Methods: From June 1 to June 15, 2012, eight Citrobacter freundii strains with reduced susceptibility to carbapenems were isolated from rectal swabs of hospitalized patients during active screening following the detection of a Klebsiella pneumoniae carbapenemase (KPC) -positive patient on the ward. All isolates were analyzed phenotypically and molecularly by PCR and sequencing. Genotype clustering was performed by multilocus sequence typing (MLST) analysis., Results: The isolates showed high rates of multidrug resistance profile. A phenotypic assay for carbapenemase production suggested the presence of metallo-β-lactamase (MBL). The blaVIM-1 gene was detected in all imipenem-resistant C. freundii isolates. MLST showed that the C. freundii isolates shared the same sequence type (ST). Phylogenetic analysis revealed a strict relationship with an ST5C. freundii isolate from a diarrhea patient in China., Conclusions: Our findings showed that the active surveillance program for CPE was useful, not only for the detection of KPC-producers, but also to identify and control the spread of other MDR pathogens that could expand the spectrum of circulating MDR pathogens., (Copyright © 2013 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
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