399 results on '"G. Olivieri"'
Search Results
2. Lasers efficacy in pain management after primary and secondary endodontic treatment: a systematic review and meta-analysis of randomized clinical trials
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Firas Elmsmari, Hafsa Shujaie, Roaya Alzaabi, José A. González, Tareq Aljafarawi, Juan G. Olivieri, Carlos A. Jurado, and Kelvin I. Afrashtehfar
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Dental laser applications ,Dental pain ,Dental pulp diseases ,Diode laser ,Endodontic pain relief ,Endodontic treatment ,Medicine ,Science - Abstract
Abstract Postoperative pain is a common concern following root canal treatments (RCT), impacting both patients and oral health practitioners. This systematic review and meta-analysis aimed to evaluate the effectiveness of laser treatment modalities in reducing postoperative pain compared to conventional methods after primary and secondary RCT in permanent mature teeth. A search of three electronic databases (PubMed, ScienceDirect, and The Cochrane Library) was conducted, using a broad range of keywords and terms. Gray literature and manual searches were conducted to complement the search. The inclusion criteria included randomized clinical trials based on the objective of the secondary study. A minimum sample size of 10 participants per group and a clearly defined criterion for postoperative pain assessment were required. The characteristics of the included studies were presented as tables. The Cochrane collaboration tool RoB 2.0 was used to assess the risk of bias within each study. Two reviewers extracted the data and assessed the studies independently, and discrepancies were resolved through consultation with a third reviewer. A random-effects model was employed for meta-analysis to estimate the overall effect measure. Heterogeneity was evaluated using Cochran’s Q test and the I 2 index. Publication bias was explored via Funnel plots and Egger’s test. Subgroup analyses and meta-regression were conducted to assess variations among laser methods and examine the influence of independent factors. The significance threshold for all analyses was set at 5% (α = 0.05). Intraoral laser therapy demonstrated no significant advantage over conventional treatments but consistently outperformed placebo, particularly from 4 to 72 h post-treatment. Low-level laser therapy provided slight pain reduction in the first 8 h, though its effectiveness diminished in retreatment scenarios. Photodynamic therapy and laser disinfection showed marginal benefits, especially shortly after treatment, with reduced efficacy in longer-term or retreatment contexts. Further research is needed to explore different applications of laser modalities and assess distinct prognostic factors in more detail.
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- 2024
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3. Coffee Silverskin as a Renewable Resource to Produce Butanol and Isopropanol
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A. Procentese, F. Raganati, L. Navarini, G. Olivieri, M.E. Russo, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Coffee is the second largest traded commodity after oil and large amounts of by-products are generated in the coffee industry every year. In particular, coffee silverskin (CS) and spent coffee grounds (SCG) are the main coffee industry residues. CS is about 4.2% (w/w) of coffee beans and the valorisation of this waste through the biorefinery approach may boost the circular economy development. In the present contribution, CS was pretreated with one of the mainly investigated biomass pretreatment reported in literature: alkaline hydrolysis in NaOH solutions. After enzymatic hydrolysis of the pretreated CS, the obtained sugars were used as carbon source to produce butanol and isopropanol by Clostridium beijerinckii DSM 6423. Moreover, fermentation tests were also carried out with synthetic media to investigate the effects of the alkaline pretreatment on the fermentation process. When pretreated CS was used as feedstock, the largest yields were 0.16 and 0.31 gsolvent/gsugars isopropanol and butanol, respectively. The reported results foster further studies regarding the reuse of CS for solvents production through fermentation processes.
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- 2018
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4. Microalgae as New Sources of Starch: Isolation and Characterization of Microalgal Starch Granules
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I. Gifuni, G. Olivieri, I. Russo Krauss, G. D'Errico, A. Pollio, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Starch is a very important biopolymer used in the modern society. It is a basic element of our diet (35% of daily calories in UE and USA). Moreover, it is widely used in the non-food industries. Indeed, the market of the non-food applications is nowadays growing and it includes chemical additives, bulking agents, and bioplastics productions (e.g. Mater-Bi by Novamont). The key starch features for industrial applications are: size and shape distributions of starch granules, crystallinity, amylose-amylopectin ratio, thermal properties. These features are critical to address the starch processing in the industrial production. Nowadays the main sources of small starch granules that fulfil the industrial requirements are maize, wheat, rice, oats, and amaranth. However, the reduced availability of arable lands and the increase of food demand ask for alternative sources for starch not in competition with food cultures. Microalgae are considered a novel highly efficient starch producers. Their starch content can reach the 40%W. They do not require arable land and fresh water for their cultivation. Nevertheless, limited information is available in literature about physico-chemical characterization of microalgal starch. Therefore, additional analysis about molecular weight, crystallinity, and amylose fraction are required to validate the potential industrial applications of this starch type. The present contribution reports a study on starch granules present in the microalga Chlorella sorokiniana. The granules were isolated and a preliminary physico-chemical characterization was carried out. The microalgal starch was characterized by small granules of about 1 µm with a narrow size distribution (key feature for some applications). The molecular weight of microalgal starch is comparable with that of plant- starch sources. The amylose content and crystallinity pattern were similar to cereal starch. Moreover, the high gelatinization temperature of 110 °C makes these granules suitable for system requiring high processing temperature such as for biodegradable materials.
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- 2017
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5. Biodiesel Production in Outdoor Cultures of Scenedesmus Vacuolatus
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I. Gargano, G. Olivieri, R. Andreozzi, R. Marotta, A. Marzocchella, and A. Pollio
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The supply of renewable feedstocks for the production of convenience goods combined with the carbon capture and storage is considered a promising solution to both fossil resources depletion and global warming control. Photosynthetic microorganisms, e.g. microalgae, are good candidates for this challenging bet. The culture performances remarkably reduce when microalgal cultures move from laboratory to industrial scale. The basic requisite for the development of large-scale production of oil from microalgae is to make the process environmentally and economically sustainable/feasible. A key issue for the process based on outdoor photobioreactors (PBR) is the efficient utilization of the photosynthetically active radiation (PAR). The present work reports the results of outdoor cultures of Scenedesmus vacuolatus strain ACUF 053/95 in1.7 L inclined bubble column (IBC) photobioreactors characterized by 250 cm2 irradiated surface. Cultures were carried out in outdoor: i) during the May-July period under shadow conditions, irradiance maximum of 450 µE/(m2 s); ii) during the May-July period under direct sun light, irradiance maximum of 2100 µE/(m2 s); iii) during the September-November period under direct sun light, irradiance maximum of 2000 µE/(m2 s). Harvested microalgae were processed to characterize the biodiesel composition thought direct alkaline transesterification. The biomass concentration at steady state conditions was kept within the range 3-4 g/L. Biomass volumetric productivity ranged in the interval 0.17-0.22 g/(L d) - area specific productivity of 11-15 g/(m2 d) - depending on the operation mode.
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- 2016
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6. Butanol Production by Fermentation of Fruit Residues
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F. Raganati, A. Procentese, G. Olivieri, M.E. Russo, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The feedstock of the Acetone-Butanol-Ethanol (ABE) fermentation is a key issue for the economic success of the biotechnological route to produce biobutanol. Residues from agro-alimentary industries are particularly interesting as renewable substrates for the ABE fermentation because they are abundant and un-competitive with food sources. The residues are also a pressing issue for industries because more than 50% of the processed feedstocks are discharged and their disposal is particularly expensive. However, the high fraction of sugars of the residues makes them a promising interesting feedstock for the production of butanol. This contribution is about the characterization of the ABE fermentation by Clostridium acetobutylicum DSM 792 using sugars from fruit peels. Apple and pear peel extracts were tested as substrate for the fermentation. Batch tests were carried out under a wide interval of peels to water mass ratio. The conversion process was characterized in terms of metabolites and cell production, sugars conversion, specific rate of butanol production and of sugar consumption, butanol and cell yields. The fermentation tests with feedstock peels to water mass ratios lower than 1/6 were characterized by total sugar conversion and low butanol concentration (
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- 2016
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7. Succinic Acid Production from Hexoses and Pentoses by Fermentation of Actinobacillus Succinogenes
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M. Ferone, F. Raganati, G. Olivieri, P. Salatino, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Succinic acid is a precursor for many chemicals in the food, pharmaceutical, cosmetic, and biodegradable plastics industries. Nowadays, the biotechnological route to produce succinic acid is focused on processes based on the fermentation of natural materials characterized by high fraction of carbohydrates. Actinobacillus succinogenes is proposed in the literature as a promising strain for the production of succinic acid. Indeed, A. succinogenes may utilize a wide spectrum of carbohydrates as carbon sources. Moreover, the CO2 required for the fermentation allows to include the biotechnological route among the carbon capture and conversion processes. A key issue of fermentation processes aimed at producing succinic acid is the feedstock cost. Pre-requisites of the feedstock are: availability at high mass flow rate over all the year, and non-competitive with (human and animal) food. Lignocellulosic biomass is a potential feedstock because it fulfils the listed pre-requisites. This contribution reports the characterization of succinic acid fermentation by A. succinogenes 130Z adopting as carbon source sugars representative of the hydrolysis of lignocellulosic biomass: glucose, mannose, arabinose, and xylose. Batch fermentation tests were carried out using single sugar as carbon source for a systematic characterization of the conversion process of the investigated sugars. The conversion process was characterized in terms of concentration of biomass, sugars, and acids as well as of pH. The time-resolved data were processed to assess the sugar conversion, the succinic acid yield, and the productivity for each of the investigated sugars. A. succinogenes was able to convert all investigated sugars into succinic acid using MgCO3 as an indirect CO2 source. Glucose was the sugar characterized by the best performance when the initial concentration was set at 40 g/L. The performances did not depend on the investigated sugars if the initial concentration was quite low (
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- 2016
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8. Analysis of the Energy Efficiency of some Butanol Recovery Processes
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L. Salemme, G. Olivieri, F. Raganati, P. Salatino, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
This work presents an energetic analysis of three butanol recovery processes from acetone-butanol-ethanol (ABE) fermentation broth. The processes were based on: 1) gas stripping, 2) liquid-liquid extraction, and 3) supercritical extraction. Material and energy balance simulations were carried out by commercial software Aspen Plus®. Processes were characterized in terms of energy efficiency and specific energy requirement. Operating conditions – e.g. gas stripping flow rate and solvent flow rate - were investigated and optimized with respect to the energy objective functions.
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- 2016
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9. The Cyanobacterial Route to Produce Poly-ß-hydroxybutyrate
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R. Carpine, G. Olivieri, K. Hellingwerf, A. Pollio, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The amount of plastics produced in the world and released in the environment has dramatically increased putting societies and the environment to hard test. The biodegradable plastics are a potential solutions to reduce the environmental impact and the fossil resource exploitation. Polyhydroxyalkanoates (PHAs) are widely adopted as building blocks for biodegradable plastic production and they may be produced by microorganisms. The poly-ß-hydroxybutyrate (PHB) is the most widespread and thoroughly characterized PHA found in bacteria. In particular, cyanobacteria are potential host systems for the PHB production, as they may synthesize this polymer from CO2. This contribution reports a joint research between the University of Napoli and the University of Amsterdam on the feasibility of PHB production by Synechocystis PCC6803. Both BG11 (balanced conditions) and BG0 (nitrogen-starved conditions) media were investigated to find the best growth conditions and optimal PHB production. Batch autotrophic cultures were carried out in inclined bubble column photobioreactors under 12h/12h light/dark cycles. Cultures were characterized in terms of biomass, PHB content, pH and nitrate consumption. After cell-rupture with sonication the PHB concentration was assessed by means of GC, adopting a modified propanolysis method. The maximum PHB concentration was 4 mg/L and the maximum PHB productivity was 0.19 mg/(L day). The PHB content of the cells is definitively higher in nitrogen-starved medium (1 %) than in balanced medium (0.25 %).
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- 2015
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10. Immobilization of Carbonic Anhydrase for Biomimetic CO2 Capture in a Slurry Absorber as Cross-Linked Enzyme Aggregates (CLEA)
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S. Peirce, M.E. Russo, V. De Luca, C. Capasso, M. Rossi, G. Olivieri, P. Salatino, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Novel post-combustion Carbon Capture and Storage (CCS) processes include absorption of CO2 into aqueous solutions assisted by enzyme catalysis. Carbonic anhydrase EC 4.2.1.1 (CA) catalyzes CO2 hydration and it has been proposed as industrial biocatalyst for biomimetic CCS processes. The present study reports on the use of bovine CA immobilized via cross-linking of enzyme aggregates (CLEA). The aim of this study was to improve the biocatalyst stability at the typical operating conditions of CCS processes (high temperature, alkaline pH, high salt concentration). The optimum conditions of the immobilization procedure were determined in terms of enzyme concentration and cross-linker concentration. In addition, a magnetic CLEA (m-CLEA) sample was prepared, based on cross-linking in presence of amino-functionalized paramagnetic nanoparticles. Immobilization yields was remarkable in both cases. No substantial differences were observed between conventional and magnetic CLEA. The use of magnetic CLEA enables effective separation of the biocatalyst from the reaction mixture and prevent drawbacks associated with CLEA aggregation and compaction induced by centrifugation and filtration.
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- 2015
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11. Process Simulation of Biobutanol Production from Lignocellulosic Feedstocks
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A. Procentese, T. Guida, F. Raganati, G. Olivieri, P. Salatino, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
A potential flowsheet to produce butanol production by conversion of a lignocellulosic biomass has been simulated by means of the software Aspen Plus®. The flowsheet has included upstream, fermentation, and downstream sections and the attention has been focused on the upstream section. The proposed process is characterized by an economic potential, three times the depreciation rate of the main fixed investment rate, as well as environmental benefits because it is a "clean energy source".
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- 2014
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12. MFA of Clostridium Acetobutylicum Pathway: the Role of Glucose and Xylose on the Acid Formation/Uptake
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F. Raganati, A. Procentese, G. Olivieri, M.E. Russo, P. Salatino, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Acetone-Butanol-Etanol is typically produced during the second stage of batch fermentations of some Clostridium strains under selected operating conditions: acids are consumed along with the carbon source and pH increase This contribution reports a MFA regarding Clostridium acetobutylicum DSM 792 fermentation adopting reference sugars (glucose and xylose) as carbon sources. The attention on these sugars is particularly relevant because they are the main components of hydrolyzed lignocellulosic biomass. The results have pointed out that the butyrate formation pathway plays a fundamental role both in the accumulation of butyrate and in butyrate uptake without acetone formation.
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- 2014
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13. Biobutanol Production from Hexose and Pentose Sugars
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F. Raganati, A. Procentese, G. Olivieri, P. Salatino, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The Acetone-Butanol-Ethanol (ABE) fermentation is receiving renewed interest as a way to upgrade renewable resources for the production of products with high added value as chemicals and fuels. Main pre-requisites of fermentation feedstocks are abundance and un-competitiveness with food sources and they are fulfilled by lignocellulosic biomass. This contribution reports about the characterization of the ABE fermentation by Clostridium acetobutylicum DSM 792 adopting sugars representative for hydrolysis products of lignocellulosic biomass: glucose, mannose, arabinose, and xylose. Batch fermentation tests with binary mixtures of sugars were performed to assess the possible crossed/coupled effects of the investigated sugars on the fermentation performances. The mass ratio of sugars in binary mixture tests was set at 1:1 and the total initial concentration was set at 60 g/L. The conversion process was characterized as a function of the time in terms of biomass, acids, and solvents concentrations as well as of pH and total organic compounds. The simultaneously fermentation of binary mixture of sugars enhances the conversion of the investigated sugars into butanol/solvents. The xylose fermentation appears to be improved when it is mixed with the investigated sugars.
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- 2014
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14. River surface flow estimation using a camera: a case study on the Tiber River
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R. Tauro, M. Porfiri, A. Petroselli, G. Olivieri, R. Rapiti, G. Cipollari, and S. Grimaldi
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Environmental sciences ,GE1-350 ,Geology ,QE1-996.5 - Abstract
No abstract available.
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- 2015
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15. Immobilization of Carbonic Anhydrase for Biomimetic CO2 Capture
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M.E. Russo, S. Scialla, V. De Luca, C. Capasso, G. Olivieri, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Novel post-combustion treatments include carbon capture and sequestration processes based on biomimetic strategies. These strategies include CO2 absorption into aqueous solution assisted by enzyme catalysis. Carbonic anhydrase catalyses CO2 hydration and it has been proposed as industrial biocatalyst for Carbon Capture and Storage (CCS) post-combustion processes. The recombinant enzyme SspCA, isolated from the thermophile bacterium Sulfurhydrogenibium sp. was characterized as potential biocatalyst for CO2 capture processes based on regenerative absorption into alkaline solutions. This paper reports results of a preliminary study focused on the immobilization of carbonic anhydrase on granular solids to improve biocatalyst stability at the typical operating conditions of the CO2 absorption processes. This study included the selection of solid supports and of the immobilization technique. Granular fine silica particles were adopted as enzyme carriers. Two classes of solids were investigated: 120 and 9 µm d50 diameter. Bovine carbonic anhydrase was used as enzyme model in order to optimize immobilization procedure and activity assay for immobilized carbonic anhydrase. Enzyme-carrier covalent bonding was adopted as immobilization technique. In particular, solids were silanized and activated with respect to the enzyme by means of glutaraldehyde branches. For 120 µm particles, the maximum enzyme loading resulted 40±3 mg of bovine CA per g of solids and the maximum yield resulted about 66±5% for initial CA concentration between 1.4 and 4 mg/mL.
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- 2013
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16. Preliminary Assessments of Combined Effects of Surface Tension and Viscosity on Bubble Column Hydrodynamics
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G. Olivieri, S. Grillo, G. D'Errico, A. Marzocchella, M. Ruzicka, and P. Salatino
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The present contribute reports results of combined effects of liquid features on the hydrodynamics of a lab-scale bubble column. In particular, the combined effects of viscosity - Newtonian fluid – and surface tension were investigated. The experimental apparatus consisted of a 10 L bubble column: a 120 mm ID,1.8 m high Plexiglas column equipped with 0.4 mm needles gas distributor, free area 0.13%. The unit was equipped with gas flow controller, humidifier, diagnostic instrumentation, and data acquisition unit. The unit was operated batchwise with respect to the liquid phase at temperature of 25°C. The liquids investigated were aqueous solutions of sodium alginate and sodium chloride. The concentration of sodium alginate was increased up to 0.5%W, the sodium chloride up to 2 M. Solutions were characterized in terms of viscosity, surface tension, and conductivity. The viscosity ranged between 0.90 and 20 mPa•s, the surface tension between 68.4 and 71.9 mN/m, and conductivity between 0 and 5.34 S/m. Results showed that the transition velocities between hydrodynamic regimes (homogenous regime – transition regime – heterogeneous/turbulent regime) strongly depends on investigated liquid features.
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- 2013
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17. Effects of Photobioreactor Depth on Stichococcus Cultures Aimed at Biodiesel Production
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I. Gargano, G. Olivieri, R. Andreozzi, R. Marotta, A. Marzocchella, G. Pinto, and A. Pollio
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Biofuels production by microalgae is a challenging process known since the end of the last century. Bio-oil extracted from microalgae may be adopted as crude fuels or may be transesterified to biodiesel. Key aspects for industrialization of microalgae-to-biofuel processes are: selection and improvements of algal strains; development of high-performance photobioreactor; improvement of oil extraction and transesterification. The present contribution regards the effects of reactor design on biodiesel production by Stichococcus bacillaris of Naegeli genus. The attention was focused on the effects of the depth of sub-horizontal flat photobioreactors on the performances of continuous cultures. Two rectangular shape columns were investigated of depth 5 and 8 cm. Semi-continuous conditions were investigated as regards the biomass suspension. Tests were carried out at 23°C and pH 7. The continuous irradiance level was set at 240 µE m-2s-1, gas flow rate at 0.5 vvm, CO2 concentration in the gas stream set at 2%v. Cultures were characterized in terms of pH, concentration of total nitrogen, total organic carbon, total inorganic carbon, biomass, lipids fraction and methyl-ester distribution of transesterified lipids. Results pointed out that the performance of the photobioreator decreased with the photobioreactor depth: the specific productivity referred to the irradiated surface increased when the light path decreased.
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- 2013
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18. Photobioreactors for Microalgal Cultures: a Model for Photosynthesis Rate Assessment
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G. Olivieri, A. Marzocchella, P. Salatino, L. Gargiulo, P. Lettieri, and L. Mazzei
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
A model aimed at the assessment of photobioreactor performances was developed. It was focused on coupling photosynthesis kinetics and photobioreactor hydrodynamics. A lumped kinetic parameter model of photosynthetic factor was adopted to relate local irradiance and photosynthetic rate. Hydrodynamics was modelled according to a Lagrangian approach based on isotropic turbulence hypothesis. The photobioreactor performances - expressed in terms of local and global photosynthesis rate - were assessed with reference to a flat photobioreactor configuration. Irradiance level and biomass concentration were changed in the typical range of operating conditions adopted for processes reported in the literature. Results showed that set the biomass concentration, the photosynthesis rate may be optimized by tuning the level of wall irradiance.
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- 2013
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19. Butanol Production by Clostridium acetobutylicum in a Continuous Packed Bed Reactor Fed with Cheese Whey
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F. Raganati, G. Olivieri, M.E. Russo, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
This research work reports on the feasibility of bio-butanol production by fermentation of cheese whey in a continuous packed bed reactor (PBR). The anaerobic solventogenic bacterium Clostridium acetobutylicum DSM 792 was adopted for the fermentation processes and commercial cheese whey powder was adopted as substrate. The fermentation plant consisted of a PBR, liquid pumps, a thermostatic unit, and a pH control unit. The PBR was a 4 cm ID, 16 cm high glass tube with a 8 cm bed of 3 mm Tygon rings, as carriers. The pH ranged between 4.5 and 5.5, the dilution rate (D) between 0.4 h-1 and 0.64 h-1. The PBR feedstock was asolution of deproteinized cheese whey powder. Results show that the best performance (butanol productivity 2.66 g/Lh, butanol concentration 4.93 g/L, butanol yield 0.26 g/g, butanol selectivity of the overall solvents production 82%w) was at D=0.54 h-1.
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- 2013
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20. Non-linear Analysis of Feedback Controlled Aerobic Cultures
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G. Olivieri, M.E. Russo, E. Mancusi, A. Marzocchella, P.L. Maffettone, and P. Salatino
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
In the present work, the results of a complete dynamical analysis of open- and closed-loop continuous bioreactor for cultures of substrate inhibited aerobic microorganisms were presented. In the closed loop system the dissolved oxygen concentration was controlled at a pre-set value by means of a proportional-integral feedback control scheme manipulating the inlet substrate concentration. A mathematical model was developed coupling the mass balance equation for the substrates (carbon-source and oxygen) and the controller. Solutions of both open- and closed-loop systems were assessed by means of parametric continuation technique and bifurcational analysis tools. The dynamics behavior was studied by changing the main operating parameters – dilution rate and gas-liquid mass transfer rate - and the controller parameters - gain and set-point – in a wide intervals. We have found that the regimes strongly depend on the operating conditions. In particular, the open-loop unstable steady state may be stabilized in closed-loop system, provided the proper choice of both the gain and the reset time for the selected set-point value of oxygen concentration.
- Published
- 2013
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21. Kinetic Assessment of Thermostable Carbonic Anhydrase for CO2 Capture Processes
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M.E. Russo, G. Olivieri, M. Rossi, C. Capasso, V. De Luca, A. Marzocchella, and P. Salatino
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The most recent challenge to reduce greenhouse gas emissions is the development of Carbon Capture and Sequestration (CCS) processes for CO2 removal from flue gases. The biomimetic strategy is based on the adoption of Carbonic Anhydrase (CA) as an industrial biocatalyst as an alternative to conventional additives (e.g. amines) to increase CO2 absorption rate in aq. solutions. The present contribution concerns the kinetic assessment of a recombinant CA (SspCA) identified and characterized in the thermophile bacterium Sulfurihydrogenibium sp YO3AOP1. The CA characterization - long term stability included - was carried out under operating conditions close to those typically adopted in CCS plants. The absorption rate of pure CO2 into aq. solutions was assessed by working out time-resolved measurements of gas pressure decay in a batch stirred reactor. The first order enzyme kinetics for SspCA was assessed at 25 °C in buffer at pH 9.6. Long term stability of SspCA at 40 and 70 °C was promising compared with that of CA from bovine erythrocytes.
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- 2012
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22. Effects of CO2 and pH on Stichococcus bacillaris in Laboratory Scale Photobioreactors
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G. Olivieri, I. Gargano, R. Andreozzi, R. Marotta, A. Marzocchella, G. Pinto, and A. Pollio
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The effects of both the CO2 concentration and the pH of the medium on biodiesel production by Stichococcus bacillaris (ACUF 158/11) were reported. Indoor cultures of S. bacillaris were carried out in both 0.6 L vertical bubble column photobioreactors and 1.7 L inclined bubble column photobioreactors. Batch, fed-batch and semi-continuous conditions were investigated as regards the biomass cultivation with Bold Basal Medium. pH was changed between 3 and 7. Tests were carried out at 23 °C, 200 µE/(m2 s). The CO2 concentration in the gas stream fed at the photobioreactors (0.5 vvm) was increased up to 15 %v to stimulate the growth process and to simulate the CO2 level in exhaust gas. Preliminary results showed that CO2 concentration higher than the air (0.035 %v) improved the process performances in terms of productivity and concentration of lipids. No strong differences were observed for the selected CO2 concentrations (5, and 15 %v). Tests at different pH carried out with 5 % CO2 supplemented air showed that pH of about 7 maximize the biomass productivity. Tests in inclined bubble columns resulted in a significant higher biomass and oil productivity (256 and 80 mg/L d) than those assessed in vertical bubble column (124 and 42 mg/L d).
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- 2012
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23. Butanol Production from Lignocellulosic-based Hexoses and Pentoses by Fermentation of Clostridium Acetobutylicum
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F. Raganati, S. Curth, P. Goetz, G. Olivieri, and A. Marzocchella
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The Acetone-Butanol-Ethanol (ABE) fermentation is receiving renewed interest as a way to upgrade renewable resources into valuable base chemicals and liquid fuels. Abundance and un-competitiveness with food sources are desired features of a potential substrate, and they are met by lignocellulosic biomass. This contribution is about the characterization of the ABE fermentation by C. acetobutylicum DSM 792 using sugars representative for hydrolysis products of lignocellulosic biomass: hexoses (glucose and mannose) and pentoses (arabinose and xylose). Batch fermentations of single sugars were carried out. The conversion process was characterized as a function of time in terms of biomass, acids and solvents concentrations as well as pH and total organic compounds. C. acetobutylicum was able to convert all investigated sugars. The conversion into solvents was strongly enhanced by the presence of CaCO3 in the fermentation medium.
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- 2012
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24. A TECHNO-ECONOMIC ASSESSMENT OF BIOFUELS PRODUCTION BY MICROALGAE
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A. Marzocchella, R. Andreozzi, G. Bartalini, E. Filippone, G. Olivieri, G. Pinto, and P. Salatino
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Abstract preview not available - see full-text PDF article.
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- 2010
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25. PRODUCTION OF BUTANOL IN A CONTINUOUS PACKED BED REACTOR OF CLOSTRIDIUM ACETOBUTYLICUM
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F. Napoli, G. Olivieri, A. Marzocchella, M.E. Russo, and P. Salatino
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Abstract preview not available - see full-text PDF article.
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- 2010
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26. CHARACTERIZATION OF DYES BIOSORPTION ON FUNGAL BIOMASS
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M.E. Russo, A. Marzocchella, G. Olivieri, V. Prigione, P. Salatino, V. Tigini, and G.C. Varese
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
Not available.
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- 2009
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27. VALUTAZIONE COMPARATIVA DI DUE METODI AUTOMATICI PER LO SCREENING DI TREPONEMA PALLIDUM
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L. Cianci, F. Milano, M. Pelagi, G. Olivieri, P. Verza, and G.C. Guazzotti
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Microbiology ,QR1-502 - Published
- 2006
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28. S13.1 Developing systemic autoimmune diseases in healthy subjects persistently positive for antiphospholipid antibodies: long-term follow-up study
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F Ceccarelli, F Natalucci, G Olivieri, C Pirone, L Picciariello, V Orefice, FR Spinelli, C Alessandri, A Chistolini, and F Conti
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- 2022
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29. PO.7.148 Patients with systemic lupus erythematosus and their experience with vaccination against COVID-19: a descriptive and explanatory study
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F Ceccarelli, V Covelli, G Olivieri, F Natalucci, C Alessandri, and F Conti
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- 2022
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30. Delay-sensitive power estimation at the register-transfer level.
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Davide Bruni, G. Olivieri, Alessandro Bogliolo, and Luca Benini
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- 2001
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31. P44 PREDICTORS OF MORTALITY AFTER ACUTE TYPE A AORTIC DISSECTION REPAIR IN PATIENTS OF 70 YEARS OF AGE OR OLDER
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F Settepani, A Cappai, A Cannata, B Merlanti, M Carrozzini, G Olivieri, A Costetti, and C Russo
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Cardiology and Cardiovascular Medicine - Abstract
Objectives The impact of age on outcomes of acute type A aortic dissection remains controversial. We sought to investigate 30–day and follow–up outcomes of acute type A aortic dissection repair in elderly patients. Survival anlysis was performed and independent risk factors for mortality were searched. Methods During a 21–year period (2000–2021), 102 patients of 70 years of age or older were emergently treated for acute type A aortic dissection at our institution. (mean age 75.1 ± 3.8 years, 53.9% male) Preoperative characteristics are displayed in Table I. Results Overall 30–day mortality (including operative mortality) was 21.6%. Mortality increased among patients presenting with atrial fibrillation (adjusted odds ratio 10.33, P = 0.02) and visceral malperfution (adjusted odds ratio 70.88, P = 0.005). Survival at 1, 5 and 10 years was 70.2 ± 4.6%, 50.9 ± 5.6% and 24.1 ± 5.6%, respectively (Figure 1). The need to perform concomitant coronary artery bypass grafting was the only independent risk factor for mortality during follow–up. (adjusted odds ratio 2.21, P = 0.04) Conclusions Thirty –day and follow–up mortality were extremely satisfactory in elderly patients undergoing acute type A dissection repair. We could detect significant disease and surgery–related risk factors for death. Patients presenting with atrial fibrillation or visceral malperfusion had the poorest 30–day outcome. The need to perform concomitant coronary artery bypass grafting, significantly increased the mortality during follow–up.
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- 2022
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32. A novel integrated fermentation/recovery system for butanol production by Clostridium acetobutylicum
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F. Raganati, A. Procentese, G. Olivieri, M.E. Russo, P. Salatino, A. Marzocchella, Raganati, F., Procentese, A., Olivieri, G., Russo, M. E., Salatino, P., and Marzocchella, A.
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Bio Process Engineering ,Biofilm fixed bed reactor ,Process Chemistry and Technology ,General Chemical Engineering ,Butanol ,Adsorption column ,Energy Engineering and Power Technology ,Integrated recovery ,Clostridium acetobutylicum ,Continuous fermentation ,General Chemistry ,Industrial and Manufacturing Engineering - Abstract
Intensive butanol production was carried out by immobilized cells of Clostridium acetobutylicum in a novel continuous fermentation system. The fermentation system consisted of four packed bed biofilm reactors (PBBR) connected in series. The novelty of the proposed system is: i) the use of a novel stagewise simulated moving bed (SMB) design/operational mode; ii) the integration of the reaction and the product recovery sections. The SMB operational mode was accomplished by equipping the PBBR with manifolds and time-actuated switching valves. PBBR integration with the product recovery section was also tested. An adsorption column was operated between the 3rd and 4th reactor of the series. The tuning of the PBBR system overall dilution rate (DOV) allowed to maximize: i) sugar conversion (DOV
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- 2022
33. AB0119 ROLE OF COSTIMULATORY MOLECULES IN SYSTEMIC LUPUS ERYTHEMATOSUS: FOCUS ON CD137
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F. Natalucci, A. Di Filippo, F. Ceccarelli, I. Zizzari, G. Olivieri, V. Orefice, C. Pirone, F. R. Spinelli, C. Alessandri, M. Nuti, and F. Conti
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Rheumatology ,Immunology ,Immunology and Allergy ,General Biochemistry, Genetics and Molecular Biology - Abstract
BackgroundSystemic Lupus Erythematosus (SLE) is a chronic autoimmune disease characterized by a wide autoantibodies production. The traditionally concept of a B-cell driven disease has been changed in the last years due to the evidence demonstrating the crucial role of T cells in SLE pathogenesis. In particular, regulatory (Treg) and memory T cells seem act through co-stimulatory and co-inhibitory molecules, such as CD137, PD1-1 and CTLA4. The over-expression of this molecules on lymphocytes may contribute to immune system dysregulation.ObjectivesThe primary objective of the present case-control study was to evaluate the expression of CD137, PD1-1 and CTLA4 on T cell surface of SLE patients by using flow-cytometry. Secondly, we evaluated the percentage of Treg and memory T cells.MethodsWe enrolled patients SLE patients (2019 ACR/EULAR criteria) and sex/age-matched healthy subjects (HS). Demographic, clinical, and laboratory data were collected in a standardized computerized electronically filled form. Disease activity was assessed by SLEDAI-2k. Each subject underwent peripheral blood sample collection. By using flow-cytometry we evaluated the expression of FOXP3, CD137, PD1-1 and CTLA4, CD45, CD25, CCR7 to determine the percentage of Treg and memory T cells.ResultsThe present analysis included 21 SLE patients [M/F 1/20 median age 48 years (IQR 17), median disease duration 144 months (IQR 204)]. The Treg percentage was significantly lower in SLE compared to HS [median 4.2 (IQR 0.32) versus 2.5 (IQR 2.44); p=0.001, Figure 1A]. Moving on effector Treg (eTreg), SLE patients with high disease activity (SLEDAI > 4) showed a significantly higher prevalence for these cells compared to patients with SLEDAI ≤ 4 [1.16 (IQR 0.51) versus 0.53 (IQR 0.8), p=0.014, Figure 1B]. Moreover, inverse correlation was found between eTreg percentage and SLEDAI-2k [p=0.029, r=-0.47 (CI 0.75 – 0.04) Figure 1C]. The evaluation of CD137 expression was significantly higher in SLE patients compared to HS on CD3+ cells [median 5.32 (IQR 6.11) versus 3.3 (IQR 1.7), p=0.001, Figure 1F]. On CD4+ cells, CD137 expression positively correlated with disease activity [p=0.0082, r=0.58 (CI 0.15-0.82)]. Finally, when analysing memory T cells subpopulations, inverse correlation has been found between effector memory T cells (TEM, CD45RA-CCR7-) and SLEDAI-2k when considering CD3+ [p=0.029, r=-0.56 (CI 0.81 – 0.12)] and CD4+ cells [p=0.016, R=-0.54 (CI -0.80 - -0.1)]. Of note, CD137 expression on T central memory cells (TCM, CD45RA-CCR7+) positively correlated with SLEDAI-2k [(p=0.019, r=0.52 (CI 0.09 – 0.79)].Figure 1.A) Comparison of the percentage of Treg in HS and SLE patients. B) Comparison of the percentage of eTreg in SLE patients with high disease activity and low disease activity C) Correlation between % eTreg and SLEDAI-2k. D) Comparison of the percentage of CD3+CD137+ cells in HS and SLE patients. E) Comparison of % of CD4+CD137+ cells in SLE patients with high disease activity and low disease activity F) Correlation between % of CD4+CD137+ cells and SLEDAI-2k.ConclusionOur results suggest a possible role of CD137-CD137L axis in SLE pathogenesis. The stimulatory role of this molecule is indicated by the positive correlation between SLEDAI-2k values and surface expression of CD137. Moreover, inverse correlation between SLEDAI-2k and eTreg percentage suggests a possible Treg dysregulation in SLE.Table 1.SLE cohort featuresClinical and Laboratory FeaturesMucocutaneous80.9%Articular76.1%Serositis19.0%Kidney23.8%Haematological48.2%CNS/PNS9.5%Thrombotic events4.7%anti-dsDNA68.4%anti-SSA/anti-SSB47.6%anti-RNP19.0%anti-Sm33.0%Antiphospholipid antibodies14.2%Low C3/C457.1%Previous TherapyGlucocorticoid90.5%Hydroxychloroquine95.2%Methotrexate23.8%Mofetil Mycophenolate33.3%Ciclosporin28.5%Cyclophosphamide9.5%Azathioprine33.3%Rituximab14.3%Antiplatelet23.8%Anticoagulant therapy4.7%Disclosure of InterestsNone declared
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- 2022
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34. The efficiency of transvaginal oocyte aspiration in Remonta Argentino jennies
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AA Ferrante, CC Arraztoa, JP Plaza, A Gambini, C Baca Castex, MB Rodriguez, O Brisky, S Bruno, G Olivieri, MH Miragaya, and DM Neild
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Equine - Published
- 2022
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35. POS1058 BIOLOGICAL DISEASE-MODIFYING ANTI-RHEUMATIC DRUGS IN PSORIATIC ARTHRITIS: PREDICTORS OF TREATMENT SURVIVAL IN A REAL-LIFE SETTING
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F. Ceccarelli, F. Natalucci, G. Olivieri, G. Galasso, C. Pirone, V. Orefice, C. Garufi, F. R. Spinelli, R. Scrivo, C. Alessandri, and F. Conti
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Rheumatology ,Immunology ,Immunology and Allergy ,General Biochemistry, Genetics and Molecular Biology - Abstract
BackgroundPsoriatic arthritis (PsA) is a chronic, immune-mediated, inflammatory arthropathy, distinctively involving joints and enthesis. The improved understanding of PsA pathogenesis has enabled the development of biological disease-modifying anti-rheumatic drugs (bDMARDS) targeting specific cytokines and signalling pathways. The availability of these drugs deeply modified PsA history, by preventing disease progression and by improving quality of life. Nevertheless, bDMARDS are not effective in all the patients who may experience primary or secondary inefficacy or adverse events development.ObjectivesIn a real-life scenario, we evaluated the bDMARDs retention rate in a large PsA cohort. In detail, we compared drug survival of the first and second biological drug and we investigated the presence of factors associated with the bDMARDs treatment withdrawal.MethodsWe longitudinally evaluated adult PsA patients (2016 CASPAR criteria) treated with at least one bDMARD during disease history. For each PsA patient, the clinical and laboratory data, including demographics, past medical history with the date of diagnosis and treatments, clinical disease phenotypes, were collected in a standardized, computerized and electronically filled form. The retention rate was estimated by using the Kaplan-Meier method. Predictors for bDMARDs withdrawal were investigated in univariate and multivariate Cox proportional hazards analysis, adjusted for relevant variables.ResultsThe present analysis included 223 PsA patients [M/F 91/132; median age 57 years (IQR 17); median disease duration 120 months (IQR 132)]. Adalimumab and Etanercept were the most frequently prescribed bDMARDs (41.5% and 41.0%, respectively). The retention rate of the first prescribed bDMARD as reported in Figure 1A: in detail, we found a 12-months retention rate of 79.8%. The comparison between etanercept and adalimumab showed a similar 12-months retention rate, but significantly higher for etanercept at 24 months (82.4% versus 69.5%, p=0.0034, Figure 1B). Out of 223 enrolled PsA patients, 109 (48.9%) received only one bDMARDs, while the remaining 114 (51.1%) received at least 2 drugs. When comparing these two groups of patients, drug survival at 24 months was significantly higher in patients treated with one bDMARD in comparison with those treated with at least two drugs (67.7% versus 52.2%, p=0.03, Figure 1C). Finally, female sex and anxiety-depressive disorders were significantly associated with the treatment with at least two dDMARDs (p=0.005 and p=0.01, respectively).ConclusionThe results of the present study demonstrated a higher retention rate in the first-line bDMARDs treatment in comparison with second-line. Female sex and anxiety-depressive disorders may negatively affect drug retention rate.Disclosure of InterestsNone declared
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- 2022
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36. AB0118 PORPHYROMONAS GINGIVALIS AMOUNT IN THE TONGUE BIOFILM IS ASSOCIATED WITH EROSIVE ARTHRITIS IN SYSTEMIC LUPUS ERYTHEMATOSUS
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F. Ceccarelli, M. Saccucci, F. Natalucci, G. Olivieri, E. Bruni, R. Iacono, T. Colasanti, G. Di Carlo, C. Alessandri, D. Uccelletti, P. Russo, A. Pilloni, F. Conti, and A. Polimeni
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Rheumatology ,Immunology ,Immunology and Allergy ,General Biochemistry, Genetics and Molecular Biology - Abstract
BackgroundSeveral data have demonstrated the occurrence of erosive arthritis in Systemic Lupus Erythematosus (SLE) patients. However, few studies have focused on the pathogenic mechanisms involved in this feature. The implication of oral pathogens has been proved in Rheumatoid Arthritis: in particular, Porphyromonas gingivalis (Pg), by inducing citrullination, could trigger autoimmune response.ObjectivesWe evaluated amount of Pg of the tongue in a cohort of SLE patients with arthritis, focusing on the association with the erosive phenotype.MethodsSLE patients with arthritis were enrolled. We evaluated the presence of ACPA and RF by using commercially enzyme-linked immunosorbent assay kits. SLEDAI-2k was applied to assess disease activity and DAS28 to assess joint inflammatory status. Erosive damage was evaluated by ultrasound at level of metacarpophalangeal and proximal interphalangeals joints. All subjects underwent a tongue cytologic swab in order to quantify the amount of Pg (real-time PCR). The bacterium expression was obtained from the ratio between the patient’s DNA amount and that obtained from healthy subjects.ResultsThe present analysis included 33 SLE patients (M/F 3/30; median age 47 years, IQR 17; median disease duration 216 months, IQR 180). Concerning activity at the time of the enrollment, we found a median DAS28 value of 3.8 (IQR 2.8) and a median SLEDAI-2k of 4 (IQR 5). Twelve patients (36.4%) showed US-detected erosive damage in at least one joint, significantly associated with ACPA positivity (pConclusionThe possible contribution of oral microbiota in SLE erosive arthritis was here evaluated for the first time, finding a significant association between erosive damage and higher expression of Pg at tongue level.Disclosure of InterestsNone declared
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- 2022
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37. AB0436 EFFECTIVENESS OF BELIMUMAB IN SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS: A REAL-LIFE ANALYSIS
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L. Picciariello, F. Ceccarelli, F. Natalucci, G. Olivieri, C. Pirone, V. Orefice, C. Garufi, F. R. Spinelli, R. Priori, C. Alessandri, and F. Conti
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Rheumatology ,Immunology ,Immunology and Allergy ,General Biochemistry, Genetics and Molecular Biology - Abstract
BackgroundEfficacy and safety of belimumab (BLM) in Systemic Lupus Erythematosus (SLE) patients with active disease have been demonstrated by RCTs [1,2] and confirmed by several observational studies [3-8]. Most of these data have been obtained by the use of BLM intravenous formulation (IV); on the contrary, very few findings are available on the use of the drug subcutaneous formulation (SC).ObjectivesEfficacy and drug survival of BLM have been assessed in a monocentric cohort of SLE patients, exploring any difference between the two routes of administration, IV or SC.MethodsA longitudinal study on SLE patients (according to ACR 1997 classification criteria [9]) candidates for treatment with BLM has been performed. Demographic, clinical-laboratory and therapeutic data - including glucocorticoid dosage in prednisone-equivalent - have been collected. Disease activity has been assessed by SLEDAI-2k [10]; in patients with inflammatory articular involvement, DAS28-PCR [11] has been used. In compliance with the study protocol, patients were assessed at baseline and at 3 and 12 months after starting treatment.ResultsA total of 85 patients treated with BLM were enrolled, most of whom were female (male/female 2/83), with a median age of 48 years (IQR 13) and a median disease duration of 127 months (IQR 151). Fifty-one patients (60%) were treated with IV formulation and the remaining 34 (40%) with SC route. BLM was prescribed due to the following clinical manifestations: joint involvement (61.2%), cutaneous manifestations (20.0%), renal involvement (for residual proteinuria, 5.9%), haematological modifications (5.9%), constitutional involvement (3.5%), pericarditis (1.2%), headache (1.2%). In both the formulations, joint involvement was the most frequent indication of BLM (IV: 64.7%, SC: 58.8%). Median treatment duration was 15 months (IQR 24). Moving on drug efficacy, after 3 and 12 months of follow-up BLM has determined a significant reduction of SLEDAI-2k median values (p=0.001, pversus 66.5%, p=ns). During the period of follow-up, 39 patients (45.9%) discontinued BLM: 38.4% of patients due to adverse events, 41% for primary or secondary inefficacy, 15% lost to follow-up, 5.1% for pregnancy. BLM withdrawal for adverse events was more frequent in the group of patients treated with IV formulation than SC one (25.9% versus 5.9%, p=0,0001). 11 patients switched from IV formulation to SC one after a median period of 40 months (IQR 20) without loss of efficacy or adverse events.ConclusionOur results confirm BLM efficacy also in a real-life setting. Notably, our data highlight a better drug survival in patients treated with SC formulation, mainly secondary to a less frequency of adverse events.References[1]Furie R et al. Arthritis Rheum. 2011;63(12):3918-3930.[2]Navarra SV et al. Lancet. 2011;377(9767):721-731.[3]Andreoli L et al. Isr Med Assoc J. 2014;16(10):651-653.[4]Hui-Yuen JS et al. J Rheumatol. 2015;42(12):2288-2295.[5]Collins CE et al. Lupus Sci Med. 2016;3(1):e000118.[6]Touma Z et al. Rheumatol Int. 2017;37(6):865-873.[7]Iaccarino L et al. Arthritis Care Res (Hoboken). 2017;69(1):115-123.[8]Gatto M et al. Arthritis Rheumatol. 2020;72(8):1314-1324.[9]Hochberg M.C. Arthritis Rheum. 1997;40:1725.[10]Gladman DD et al. J Rheumatol. 2002;29(2):288-291.[11]Prevoo ML et al. Arthritis Rheum. 1995;38(1):44-48.Disclosure of InterestsNone declared
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- 2022
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38. AB0455 DRUGS, AUTOANTIBODIES AND GENES CONTRIBUTE TO THE DEVELOPMENT OF CHRONIC DAMAGE IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS
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G. Olivieri, F. Ceccarelli, C. Pirone, L. Picciariello, F. Natalucci, C. Ciccacci, C. Perricone, F. R. Spinelli, C. Alessandri, P. Borgiani, and F. Conti
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Rheumatology ,Immunology ,Immunology and Allergy ,General Biochemistry, Genetics and Molecular Biology - Abstract
BackgroundGenetic contribution to development of chronic damage have been scarcely investigated in systemic lupus erythematosus (SLE). In fact, whereas most studies have looked for an association between genetic variants and SLE susceptibility or disease phenotypes, only few have focused on the relationship between these biomarkers and damage development.ObjectivesMoving from these premises, we firstly analyzed the distribution of organ damage in a cohort of SLE patients and secondly we evaluate the role of clinical and genetic factors in determining the development of chronic damage.MethodsCaucasian SLE patients, diagnosed according with 1997 ACR criteria, were enrolled, and clinical and laboratory data were collected. Based on literature data, we selected a panel of 17 SNPs of following genes STAT4, IL10, IRAK1, HCP5, MIR146a, ATG16L1, IRGM, ATG5, MIR124, MIR1279, TNFSF4, CD40. Genotyping was performed by allelic discrimination assays. A phenotype-genotype correlation analysis was performed by evaluating specific domains of SLICC Damage Index (SDI).ResultsAmong 175 Caucasian SLE patients, 105 (60%) exhibited damage (SDI ≥1) with a median value of 1.0 (IQR 3.0). The musculoskeletal (26.2%), neuropsychiatric (24.6%) and ocular domains (20.6%) were involved most frequently. The presence of damage was associated with higher age, longer disease duration, neuropsychiatric (NP) manifestations, anti-phospholipid syndrome and the positivity of anti-dsDNA antibodies. Concerning therapies cyclophosphamide, mycophenolate mofetil and glucocorticoids resulted associated with the development of damage. The genotype/phenotype correlation analysis showed an association between renal damage, identified in 6.9% of patients, and rs2205960 of TNFSF4 (p=0.001; OR 17.0). This SNP resulted significantly associated with end-stage renal disease (p= 0.018, OR 9.68) and estimated GFRFigure 1.Association between renal damage and rs2205960 of TNFSF4 (p=0.001). In addition, this SNP resulted significantly associated with the development of two specific items of SDI renal domain: estimated glomerular filtration rate (GFR) ConclusionWe showed the role of age, drugs, and autoantibody profile in determining chronic damage.Our data suggest a possible role of genetic background in determining the development of renal and neuropsychiatric damage, as demonstrated by the association with polymorphisms of TFNSF4 and MIR1279, respectively. These results agree with previous studies suggesting the involvement of TNFSF4 in Lupus nephritis and microRNA in neuroinflammation.Disclosure of InterestsNone declared
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- 2022
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39. POS0553 NEW BIOMARKERS IN RHEUMATOID ARTHRITIS: ROLE OF HOMOCYSTEINYLATED ANTI-ALPHA1 ANTITRYPSIN ANTIBODIES
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M. Speziali, F. Ceccarelli, F. Natalucci, A. I. Celia, T. Colasanti, C. Barbati, G. Olivieri, E. Balbinot, C. Ciancarella, F. M. Ucci, G. Buoncuore, G. Tripdi, F. R. Spinelli, F. Conti, and C. Alessandri
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Rheumatology ,Immunology ,Immunology and Allergy ,General Biochemistry, Genetics and Molecular Biology - Abstract
BackgroundRheumatoid Arthritis (RA) is a multifactorial, chronic, systemic, inflammatory disease that can lead to progressive joint destruction (Alamanos et al, Autoimmun Rev 2005). Positivity for Rheumatoid Factor (RF) and antibodies against citrullinated proteins (ACPA) is useful for diagnostic and prognostic purposes. Nevertheless, in about 20% of patients, it is not possible to detect the presence of these autoantibodies. This has led to the identification of new antibody specificities, such as antibodies directed against carbamylated proteins (Mastrangelo A et al, J Immunol Res 2015) and, more recently, against homocysteinylated alpha 1 antitrypsin (anti-HATA) (Colasanti T et al, J Autoimmun 2020).ObjectivesTo evaluate the prevalence of anti-HATA in a large cohort of patients with RA and their correlation with serological, clinical and erosive bone damage assessed by musculo-skeletal ultrasound (US).MethodsConsecutive outpatients with RA, diagnosed according to the 2010 ACR/EULAR criteria, were enrolled. Demographic and clinical-laboratory data were recorded, including FR and ACPA determination. Disease activity was assessed by DAS28. The presence of anti-HATA antibodies was investigated by homemade ELISA using native alpha 1 antitrypsin modified in vitro to obtain homocysteinylated alpha 1 antitrypsin. US assessment was performed at the level of bilateral metacarpophalangeal and proximal interphalangeal joints; the presence of erosions and inflammatory features was identified according to OMERACT definitions (Wakefield RJ et al, J Rheumatol 2005).ResultsThe present analysis included 91 RA patients (M/F 22/69; mean age 62 years; mean disease duration 12.5 years). Overall, the prevalence of anti-HATA was 69.2%. Anti-HATA antibodies were found in 63/91 (69.2%) of the entire patient cohort, whereas 68/91 (74.3%) patients were positive for ACPA and 63/91 (69.4%) for FR. 41.4% of patients had concomitant positivity for the three autoantibodies (FR, ACPA, anti-HATA). The analysis of patients with triple positivity for related arthritis antibodies (FR, ACPA, anti-HATA) was particularly interesting: indeed, in this subgroup, 80% of patients presented erosive damage, compared to 42.1% of patients who did not present simultaneously the three autoantibodies (p=0.0001). Patients with simultaneous positivity for RF, ACPA and anti-HATA showed a more aggressive disease phenotype (p=0.0001). Finally, a positive correlation was also found between disease activity (expressed by DAS28) and total inflammatory and erosive ultrasonographic score (p=0.005 and p=0.001, respectively).ConclusionThe results of the present study confirm a high prevalence of anti-HATA in RA patients; furthermore, patients with concomitant presence of anti-HATA, ACPA and RF showed a more aggressive disease phenotype, in terms of erosive damage. Our analysis underlines as the characterization of new antibody specificities in RA could help in the early diagnosis of this disease and in the characterization of the different severity degrees.References[1]Alamanos Y, Drosos AA. Epidemiology of adult rheumatoid arthritis. Autoimmun Rev. 2005 Mar;4(3):130-6.[2]Mastrangelo A, Colasanti T, Barbati C, Pecani A, Sabatinelli D, Pendolino M, Truglia S, Massaro L, Mancini R, Miranda F, Spinelli FR, Conti F, Alessandri C. The Role of Posttranslational Protein Modifications in Rheumatological Diseases: Focus on Rheumatoid Arthritis. J Immunol Res. 2015;2015:712490;[3]Colasanti T, Sabatinelli D, Mancone C, Giorgi A, Pecani A, Spinelli FR, Di Giamberardino A, Navarini L, Speziali M, Vomero M, Barbati C, Perricone C, Ceccarelli F, Finucci A, Celia AI, Currado D, Afeltra A, Schininà ME, Barnaba V, Conti F, Valesini G, Alessandri C. Homocysteinylated alpha 1 antitrypsin as an antigenic target of autoantibodies in seronegative rheumatoid arthritis patients. J Autoimmun. 2020 Sep;113:102470.[4]Wakefield RJ, Balint PV, Szkudlarek M, et al. Musculoskeletal ultrasound including definitions for ultrasonographic pathology. J Rheumatol 2005; 32: 2485-2487.Disclosure of InterestsNone declared.
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40. The Protek-Duo Cannula for Temporary Percutaneous Right Ventricular Support: An Early Single-Centre Experience
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M. Carrozzini, B. Merlanti, G. Olivieri, G. Bruschi, G. Tata, M. Barbiero, M. Lanfranconi, M. Mondino, and C.F. Russo
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Pulmonary and Respiratory Medicine ,Transplantation ,Surgery ,Cardiology and Cardiovascular Medicine - Published
- 2022
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41. Contributors
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Gemma Acosta, Ariel Almendral, Orlando Álvarez, Inés Aramendía, María Alejandra Arecco, Juan P. Ariza, C. Arriagada, Pedro Arriola, Pilar Ávila, Patrice Baby, Vanesa Barberón, Stéphanie Brichau, Ysabel Calderon, Mauricio Calderón, Gabriela Beatriz Franco Camelio, Horacio N. Canelo, Victor Carlotto, Barbara Carrapa, Ryan Cochrane, Gilda Collo, Eduardo Contreras-Reyes, Peter Copeland, Christian Creixell, Edward Cuipa, Federico M. Dávila, Peter G. DeCelles, Juan Díaz-Alvarado, A. Echaurren, Sebastián Echeverri, A. Encinas, Adrien Eude, Miguel Ezpeleta, Lucía Fernández Paz, D. Figueroa, Andrés Folguera, Gonzalo Galaz, Héctor P.A. García, Carmala N. Garzione, Sarah W.M. George, Matías C. Ghiglione, P. Giampaoli, Guido M. Gianni, Mario Gimenez, Johannes Glodny, E. Gobbo, Marcelo A. Gonzalez, E. Gabriela Gutiérrez, Camilo Higuera, Brian K. Horton, Sofía Iannelli, Lily J. Jackson, James N. Kellogg, Keith A. Klepeis, Federico Lince Klinger, Cullen Kortyna, Thomas J. Lapen, F. Lince-Klinger, Vanesa D. Litvak, C. López, Melanie Louterbach, Leonard Luzieux, Federico Martina, Myriam P. Martinez, F. Martínez, Joseph Martinod, Ezequiel García Morabito, Héctor Mora-Páez, Federico Moreno, Francisco Sánchez Nassif, C. Navarrete, Julieta C. Nóbile, Paul O’Sullivan, Soty Odoh, Verónica Oliveros, G. Olivieri, Sebastián Correa Otto, Mauricio Parra, Ana María Patiño, A. Paul, Mark Pecha, Stefanie Pechuan, Agustina Pesce, Stella Poma, Alice Prudhomme, Juan Carlos Ramírez, Miguel E. Ramos, Alexandra Robert, E. Rocha, E.A. Rojas Vera, Christian Romero, Gonzalo Ronda, Marcos A. Sánchez, Joel E. Saylor, Edward R. Sobel, Santiago R. Soler, Richard A. Spikings, Rodrigo J. Suárez, Christian Sue, Kurt Sundell, Tonny B. Thomsen, Jonathan Tobal, Cristian Vallejo, Roelant Van der Lelij, D. Villagomez, Laura E. Webb, Wilfried Winkler, and Gonzalo Zamora
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- 2019
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42. Structure and tectonic evolution of the Interandean and Subandean Zones of the central Andean fold-thrust belt of Bolivia
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P. Giampaoli, E. Rocha, D. Figueroa, E. Gobbo, E.A. Rojas Vera, and G. Olivieri
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Paleontology ,Paleozoic ,Proterozoic ,Sedimentary rock ,Orogeny ,Fold (geology) ,Structural basin ,Cenozoic ,Geology ,Devonian - Abstract
The Interandean and Subandean Zones of the central Andean fold-thrust belt in Bolivia have a complex Proterozoic to Cenozoic tectonic history. This work presents several regional structural transects constructed from field relationships, new well and seismic data, and a compilation of previously published thermochronological ages in order to better understand along-strike variations in shortening, kinematics, and tectonic inheritance of the fold-thrust belt. New seismic lines reveal evidence of Cambrian extension, early Paleozoic deformation related to the Famatinian orogeny, and Cenozoic synorogenic shortening in the central Andes. The kinematics of the Interandean and Subandean Zones can be characterized by basement tectonic wedging and duplex formation, with detachment folding as the primary mechanism that accommodates shortening of the sedimentary cover. Along-strike, large structures were formed by lateral growth and linkage of discrete structural segments. In the northern section of the study area, structures are more evolved and have accommodated more shortening (88% minimum shortening) as compared to their counterpart in the south (62% minimum shortening). These differences could be related to the north to south difference in exhumation ages in the Interandean Zone during Cenozoic shortening, or perhaps controlled by the Devonian to Silurian sedimentary thicknesses and pre-Andean basin development.
- Published
- 2019
- Full Text
- View/download PDF
43. AVANCES EN BIOTECNOLOGÍAS REPRODUCTIVAS APLICADAS EN ASNOS.
- Author
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J., Plaza, S., Bruno, A., Ferrante, G., Olivieri, E., Nieto, and Miragaya, M. H.
- Subjects
DAIRY cattle ,ANIMAL feeding ,GRAZING ,LIVESTOCK genetics ,ANIMAL genetics - Published
- 2021
44. Technical Note: Surface water velocity observations from a camera: a case study on the Tiber River
- Author
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Flavia Tauro, G. Olivieri, Salvatore Grimaldi, Maurizio Porfiri, and Andrea Petroselli
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Flood myth ,Benchmark (surveying) ,Flow (psychology) ,Hydrograph ,Rating curve ,Scale (map) ,Sediment transport ,Surface water ,Geology ,Remote sensing - Abstract
Monitoring surface water velocity during flood events is a challenging task. Techniques based on deploying instruments in the flow are often unfeasible due to high velocity and abundant sediment transport. A low-cost and versatile technology that provides continuous and automatic observations is still not available. LSPIV (large scale particle imaging velocimetry) is a promising approach to tackle these issues. Such technique consists of developing surface water velocity maps analyzing video frame sequences recorded with a camera. In this technical brief, we implement a novel LSPIV experimental apparatus to observe a flood event in the Tiber river at a cross-section located in the center of Rome, Italy. We illustrate results from three tests performed during the hydrograph flood peak and recession limb for different illumination and weather conditions. The obtained surface velocity maps are compared to the rating curve velocity and to benchmark velocity values. Experimental findings confirm the potential of the proposed LSPIV implementation in aiding research in natural flow monitoring.
- Published
- 2018
45. River surface flow estimation using a camera: a case study on the Tiber River
- Author
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R. Tauro, G. Cipollari, R. Rapiti, Salvatore Grimaldi, G. Olivieri, Maurizio Porfiri, and Andrea Petroselli
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lcsh:GE1-350 ,Pixel ,Frame (networking) ,lcsh:QE1-996.5 ,0207 environmental engineering ,Context (language use) ,Field of view ,02 engineering and technology ,General Medicine ,Kinematics ,Geodesy ,Frame rate ,01 natural sciences ,010305 fluids & plasmas ,lcsh:Geology ,Geography ,Particle image velocimetry ,13. Climate action ,0103 physical sciences ,020701 environmental engineering ,Image resolution ,Simulation ,lcsh:Environmental sciences - Abstract
Monitoring surface water velocity during flood events is a challenging task. Techniques based on deploying instruments in the flow are often not feasible due to high velocity and abundant sediment transport. As a consequence, flow measurement campaigns are infrequent and stream velocity observations during major floods are scarce or absent. In the last few years, large-scale particle image velocimetry (LSPIV) was proposed to overcome some of these drawbacks (Fujita et al. 1997). The novelty of LSPIV should be sought in its capacity of extracting desired kinematic information from a video of the surface streamflow. The general implementation of LSPIV can be summarized in three main sequential steps: video recording, image pre-processing, and image analysis. The video recording is the simplest phase, as it can be executed with a low-cost sport camera at a standard sampling frequency (30–60 frames per second – fps) and at full-HD image resolution (1920 × 1080 pixels). An image pre-processing step is necessary for treating video frames before the image analysis. Typical pre-processing includes pixel calibration, frame correction and frame matching, to compensate for distortions and undesired mechanical vibrations. The treated frame sequence is ultimately processed through standard PIV algorithms that return the sought velocity maps. PIV (Adrian 1991) is based on the cross-correlation of pairs of consecutive frames, in which each frame is subdivided into interrogation windows that are translated on a pixel grid. In this context, we have recently proposed a novel apparatus (Tauro et al. 2015), that includes a low-cost camera (GoPro Hero 3) installed on a telescopic hollow aluminium bar to maintain its axis orthogonal to the water surface. Its main novelty with respect to current LSPIV implementations is the use of two lasers that are installed at the ends of a 1-m pole connected to the bar. Such lasers create reference points in the field of view that are crucial for pixel calibration, which would otherwise require surveying through GPS or total stations. In this work, the experimental apparatus is utilized to study 10-year return period flood events in the Tiber River in Rome, Italy. Three tests were executed in different illumination and weather conditions, from a bridge in the centre of Rome, where the historical hydrological Ripetta crosssection is located (Fig. 1(c)). Videos were recorded at full-HD resolution and 60 fps for two minutes duration each, and they were subsequently reduced to VGA resolution and resampled to 30 fps, so that the resulting 3600 consecutive frames were spaced in time by 0.033 s. The LSPIV algorithm was applied on a sequence of 500 frames, which was extracted to guarantee a sufficient transit of floating material. Figure 1(c) illustrates the velocity maps obtained from the three tests. Velocity values of each node represent the average of velocity values estimated on the available 499 pairs of consecutive frames. The most satisfactory results are those in the middle panel of Fig. 1(c), where homogenous velocity vectors are obtained and the kinematics around and before the bridge pier are well reproduced. This should be attributed to the favourable illumination and weather conditions of Test2 and to the fact that the field of view was well centred within the river. The least satisfactory velocity map is the one corresponding to Test1 and reported in the top panel of Fig. 1(c). Test1 was performed in the evening and during rainfall. Notably, the velocities
- Published
- 2018
46. Oxidative stress as a possible mechanism underlying multi-hormonal deficiency in chronic heart failure
- Author
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Mancini, Antonio, Vergani, E, Bruno, C, Olivieri, Giorgia, Segni Di, C, Silvestrini, Andrea, Venuti, A, Favuzzi, Angela Maria Rita, Meucci Calabrese, Elisabetta, A Mancini (ORCID:0000-0002-7707-4564), G Olivieri, A Silvestrini (ORCID:0000-0002-2005-3746), A Favuzzi, E Meucci (ORCID:0000-0002-8821-8041), Mancini, Antonio, Vergani, E, Bruno, C, Olivieri, Giorgia, Segni Di, C, Silvestrini, Andrea, Venuti, A, Favuzzi, Angela Maria Rita, Meucci Calabrese, Elisabetta, A Mancini (ORCID:0000-0002-7707-4564), G Olivieri, A Silvestrini (ORCID:0000-0002-2005-3746), A Favuzzi, and E Meucci (ORCID:0000-0002-8821-8041)
- Abstract
Chronic Heart Failure (CHF) is associated with multi-hormonal derangement depicting a prevalence of catabolic vs. anabolic axes. Moreover, thyroid adaption is characterized by the reduced conversion of thyroxine to the active hormone triiodothyronine. On the other hand, hormones modulate synthesis and utilization of antioxidant systems. Therefore, hormonal failure can cause unbalance between reactive radical species and the defenses, resulting in oxidative stress (OS). OS is well described in CHF, but the relationship with the hormonal picture is not entirely known. In the present review, we firstly analyze the mechanisms of ROS production in the heart, discussing animal and human studies, and focusing on new discovered protective mechanisms such as sirtuins and fibroblast growth factor 21 (FGF21). The second section is dedicated to the role of main anabolic axes influencing antioxidant systems. Finally, we present some data supporting the hypothesis that OS could be the link between hormonal derangement and clinical outcome of CHF.
- Published
- 2018
47. Recent advances and perspectives in CO2 capture by enzymatic reactive absorption
- Author
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S. Peirce, M. E. Russo, P. Bareschino, G. Olivieri, R. Chirone, P. Salatino, and A. Marzocchella
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co2 capture ,carbonic anhydrase ,absorption - Abstract
Enzymatic reactive absorption has been investigated in the last decade as novel strategy to develop environmental friendly technology for post-combustion CO2 capture [1, 2]. The process is based on CO2 absorption in aqueous solutions, the absorption rate is enhanced by the catalytic action of the enzyme carbonic anhydrase (E.C. 4.2.1.1). At temperature lower than 40°C, CO2 hydration reaction is the slowest reaction (10-2 s-1) among those occurring during CO2 reactive absorption in conventional alkaline and amine solvents: hydroxylation rate at pH 10 ranges between 10-1 and 1 s-1 and carbamate formation rate at 3M MEA is about 103 s-1. Carbonic anhydrase is the ubiquitous enzyme devoted to CO2 hydration catalysis in Nature. Depending on enzyme form, about 10-5 M carbonic anhydrase are able to speed up CO2 hydration to bicarbonate so that first order rate constant results between 1 and 103 s-1 [1]. The present contribution reports survey of recent results on the development of CO2 capture processes based on enzymatic reactive absorption from lab to pilot scale. Main investigated issues were: kinetics of thermostable carbonic anhydrase [4], development of solid biocatalyst for industrial application through carbonic anhydrase immobilization [3], proposed reactor configurations for CO2 enzymatic reactive absorption [1, 2], theoretical models CO2 enzymatic reactive absorption unit [5].
- Published
- 2017
48. Flow monitoring with a camera: a case study on a flood event in the Tiber River
- Author
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Salvatore Grimaldi, Andrea Petroselli, Flavia Tauro, G. Olivieri, and Maurizio Porfiri
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010504 meteorology & atmospheric sciences ,Rome ,0208 environmental biotechnology ,Hydrograph ,02 engineering and technology ,Rating curve ,Management, Monitoring, Policy and Law ,01 natural sciences ,Flow measurement ,Rivers ,Environmental monitoring ,Water Movements ,Weather ,0105 earth and related environmental sciences ,General Environmental Science ,Remote sensing ,Flood myth ,General Medicine ,Pollution ,Floods ,020801 environmental engineering ,Particle image velocimetry ,Flow velocity ,Sediment transport ,Geology ,Environmental Monitoring - Abstract
Monitoring surface water velocity during flood events is a challenging task. Techniques based on deploying instruments in the flow are often unfeasible due to high velocity and abundant sediment transport. A low-cost and versatile technology that provides continuous and automatic observations is still not available. Among remote methods, large-scale particle image velocimetry (LSPIV) is an optical method that computes surface water velocity maps from videos recorded with a camera. Here, we implement and critically analyze findings obtained from a recently introduced LSPIV experimental configuration during a flood event in the Tiber River at a cross section located in the center of Rome, Italy. We discuss the potential of LSPIV observations in challenging environmental conditions by presenting results from three tests performed during the hydrograph flood peak and recession limb of the event for different illumination and weather conditions. The obtained surface velocity maps are compared to the rating curve velocity and to benchmark velocity values. Experimental findings show that optical methods should be preferred in extreme conditions. However, their practical implementation may be associated with further hurdles and uncertainties.
- Published
- 2016
- Full Text
- View/download PDF
49. EFFECTS OF SALINE STRESS IN TWO CULTIVARS OF OLEA EUROPEA L: 'ARBEQUINA' AND 'BARNEA'
- Author
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F. Vita Serman, M. Ruiz, and G. Olivieri
- Subjects
Soil salinity ,biology ,Chemistry ,food and beverages ,Horticulture ,biology.organism_classification ,Olive trees ,Salinity ,Olea ,Shoot ,Osmotic pressure ,Cultivar ,Water content - Abstract
Soil salinity is a major cause of plant stress leading to economic losses in world agriculture. The aim of this study is to provide information about the effects of salinity on the growth of young olive trees (Olea europaea L. ‘Barnea’ and ‘Arbequina’) and to identify some of the underlying physiological mechanisms. One-year-old plants were planted in 30-L pots, using a sand, perlite, peat (1:1:1) soil substrate. Each treatment was drip irrigated with a NaCl solution: 4 dS/m (25 mg NaCl); 6 dS/m (37.8 mg NaCl); 8 dS/m (50 mg NaCl) and a control treatment of 2 dS/m (no NaCl added). Growth in both cultivars decreased after a period of exposure to salinity, ‘Barnea’ being the most affected cultivar. In both cultivars leaf water potentials were reduced when NaCl concentrations were increased. The osmotic potential became more negative when salt concentrations increased, thus indicating that both cultivars perform osmotic adjustment. The relative water content (RWC) did not change with the different saline treatments, suggesting that both cultivars present mechanisms to maintain water content even under stress conditions. On the other hand, high salinity levels induce ionic imbalance given higher Na+ and Cl- concentration in leaves and roots. As a result of the accumulation of these ions, the K+ concentration decreased resulting in a low ratio of K+/Na+. Both cultivars showed symptoms of toxicity in leaves and shoots which indicates that they accumulate toxic ions in the youngest leaves. ‘Arbequina’ showed higher Cl- concentrations in their root tissues over the concentrations in leaves, suggesting that there is a mechanism of Cl- compartmentalization at stem or root level that prevents entry and accumulation of Cl- in the leaf. ‘Barnea’ leaves were more damaged than those of ‘Arbequina’ and accumulated higher Cl- concentration, indicating that ‘Barnea’ is less resistant to salinity.
- Published
- 2011
- Full Text
- View/download PDF
50. Transcriptional Response of Human Umbilical Vein Endothelial Cells to Low Doses of Ionizing Radiation
- Author
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Alessandro Panconesi, Valeria Pretazzoli, G. Olivieri, Rodolfo Negri, Vincenzo Lanza, and Giuseppe Pascarella
- Subjects
Transcriptional Activation ,Regulation of gene expression ,Umbilical Veins ,Radiation ,Kinase ,Gene Expression Profiling ,Health, Toxicology and Mutagenesis ,Endothelial Cells ,Dose-Response Relationship, Radiation ,Biology ,Cell cycle ,Radiation Dosage ,Molecular biology ,Umbilical vein ,Gene expression profiling ,Gene Expression Regulation ,Radiation, Ionizing ,biology.protein ,Humans ,Radiology, Nuclear Medicine and imaging ,Cyclin-dependent kinase 6 ,Gene ,Transcription factor ,Cells, Cultured ,Transcription Factors - Abstract
We used cDNA microarray hybridization technology to monitor the transcriptional response of Human Umbilical Vein Endothelial (HUVEC) cells to x-rays doses ranging from 2 to 200 cGy. An early time window from irradiation (4h) was selected in order to minimize the effects of the cell cycle blockage eventually induced at high doses of irradiation. Three different gene-clustering algorithms have been used to group the 4134 monitored ORF based on their transcriptional response in function of the irradiation dose. The results show that while few genes exhibit a typical dose-dependent modulation with a variable threshold, most of them have a different modulation pattern, peaking at the two intermediate doses. Strikingly even the lowest dose used (2 cGy) seems to be very effective in transcriptional modulation. These results confirm the physiological relevance of sublethal-dose exposures of endothelial cells and strengthens the hypothesis that alternative dose-specific pathways of radioadaptive response exist in the mammalian cells. 111 genes were found to be modulated at all doses of irradiation. These genes were functionally classified by cellular process or by molecular function. Genes involved in coagulation and peroxidase activity and structural constituent of ribosomes were over-represented among the up-regulated genes as compared with their expected statistical occurrence. Three genes coding for regulatory kinase activities (CDK6; PRCKB1 and TIE) are found down-regulated at all doses of irradiation.
- Published
- 2005
- Full Text
- View/download PDF
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