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This study aimed to explore the clinicopathological and prognostic significance of miR-302c-3p in glioma.The expression of miR-302c-3p in glioma tissues and normal brain tissues was measured using real-time PCR. Clinicopathological associations of miR-302c-3p expression in glioma were analyzed. The Kaplan-Meier method was used to determine the survival curves. Univariate and multivariate analysis were performed using the Cox proportional hazard analysis.The relative expression of miR-302c-3p in glioma tissues was significantly lower than that in non-neoplastic brain tissues (p0.001). The decreased expression of miR-302c-3p in glioma was positively associated with WHO grade (p0.001) and Karnofsky performance status (KPS) score (p = 0.016). We found patients with low miR-302c-3p expression had significantly poorer OS (p = 0.0057) and PFS (p = 0.0092) by Kaplan-Meier method. Furthermore, multivariate regression analysis identified low miR-302c-3p expression as an independent predictor of poor survival.Our results revealed that miR-302c-3p may serve as a tumor suppressor of malignant glioma and be used as a novel biomarker to predict the clinical prognostic of patients with gliomas.

Soil nematode communities in greenhouses with different duration of continuous cropping were investigated from October 2007 to June 2008. Total nematode populations and trophic groups were observed...

We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.