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16 results on '"Günzel, Christian"'

1. Evolving methods for rational de novo design of functional RNA molecules

Author

Hammer, Stefan, Günzel, Christian, Mörl, Mario, and Findeiß, Sven

Subjects
Quantitative Biology - Biomolecules
Abstract

Artificial RNA molecules with novel functionality have many applications in synthetic biology, pharmacy and white biotechnology. The de novo design of such devices using computational methods and prediction tools is a resource-efficient alternative to experimental screening and selection pipelines. In this review, we describe methods common to many such computational approaches, thoroughly dissect these methods and highlight open questions for the individual steps. Initially, it is essential to investigate the biological target system, the regulatory mechanism that will be exploited, as well as the desired components in order to define design objectives. Subsequent computational design is needed to combine the selected components and to obtain novel functionality. This process can usually be split into constrained sequence sampling, the formulation of an optimization problem and an in silico analysis to narrow down the number of candidates with respect to secondary goals. Finally, experimental analysis is important to check whether the defined design objectives are indeed met in the target environment and detailed characterization experiments should be performed to improve the mechanistic models and detect missing design requirements., Comment: Published at METHODS, Issue title: Chemical Biology of RNA, Guest Editor: Michael Ryckelynck

Published
2019
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7. Beyond Plug and Pray: Context Sensitivity and in silicoDesign of Artificial Neomycin Riboswitches

Author

Günzel, Christian, Kühnl, Felix, Arnold, Katharina, Findeiß, Sven, Weinberg, Christina E., Stadler, Peter F., and Mörl, Mario

Abstract

ABSTRACTGene regulation in prokaryotes often depends on RNA elements such as riboswitches or RNA thermometers located in the 5′ untranslated region of mRNA. Rearrangements of the RNA structure in response, e.g., to the binding of small molecules or ions control translational initiation or premature termination of transcription and thus mRNA expression. Such structural responses are amenable to computational modelling, making it possible to rationally design synthetic riboswitches for a given aptamer. Starting from an artificial aptamer, we construct the first synthetic transcriptional riboswitches that respond to the antibiotic neomycin. We show that the switching behaviour in vivocritically depends not only on the sequence of the riboswitch itself, but also on its sequence context. We therefore developed in silicomethods to predict the impact of the context, making it possible to adapt the design and to rescue non-functional riboswitches. We furthermore analyse the influence of 5′ hairpins with varying stability on neomycin riboswitch activity. Our data highlight the limitations of a simple plug-and-play approach in the design of complex genetic circuits and demonstrate that detailed computational models significantly simplify, improve, and automate the design of transcriptional circuits. Our design software is available under a free licence on GitHub (https://github.com/xileF1337/riboswitch_design).

Published
2021
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8. Präklinische Reanimationen im Rettungsdienstbereich Dachau

Author

Günzel, Christian, Estner, Heidi L. (Priv.-Doz. Dr.), Neumeier, Dieter (Prof. Dr.), and Kochs, Eberhard (Prof. Dr.)

Subjects
Medizin und Gesundheit, Out-of-hospital, resuscitation, Dachau, Utstein, Reanimation, Rettungsdienst, Dachau, Utstein, ddc:610
Abstract

In der Dachauer Reanimationsstudie konnten in einem Zeitraum von 5 Jahren Ergebnisse von 539 Reanimations-Patienten erhoben werden, davon 405 kardialer Genese. Von diesen Patienten überlebten 11,6% bis zur Klinikentlassung. Im internationalen Vergleich lagen die Überlebensraten im Landkreis Dachau im oberen Niveau. Dennoch ist dieser Anteil deutlich niedriger, als die theoretisch denkbaren Überlebensraten nach Reanimation. Entscheidend ist jedoch das neurologische Ergebnis: 91% unserer Patienten wiesen ein gutes neurologisches Outcome auf. Nach wie vor schwierig ist die Versorgung traumatologischer Reanimationen, die trotz maximaler Therapie eine infauste Prognose aufwiesen. The prehospital resuscitation study in Dachau included the outcome of 539 Patients with out-of-hospital cardiac arrest, of those 405 with cardiac cause. Of these 11.6% survived till release from hospital. Compared to international studies the survival rate in the area of Dachau was in the upper level. Even though this rate is quite lower than the thinkable survival rate after out-of-hospital cardiac arrest. But most deciding is the neurological outcome: 91% of our patients had a favorable neurological outcome. Still difficult is the treatment of trauma patients with cardiac arrest, which in spite of getting maximum therapy still have a very bad prognoses.

Published
2012

9. Präklinische Reanimationen im Rettungsdienstbereich Dachau

Author

Neumeier, Dieter (Prof. Dr.), Kochs, Eberhard (Prof. Dr.), Estner, Heidi L. (Priv.-Doz. Dr.), Günzel, Christian, Neumeier, Dieter (Prof. Dr.), Kochs, Eberhard (Prof. Dr.), Estner, Heidi L. (Priv.-Doz. Dr.), and Günzel, Christian

Abstract

In der Dachauer Reanimationsstudie konnten in einem Zeitraum von 5 Jahren Ergebnisse von 539 Reanimations-Patienten erhoben werden, davon 405 kardialer Genese. Von diesen Patienten überlebten 11,6% bis zur Klinikentlassung. Im internationalen Vergleich lagen die Überlebensraten im Landkreis Dachau im oberen Niveau. Dennoch ist dieser Anteil deutlich niedriger, als die theoretisch denkbaren Überlebensraten nach Reanimation. Entscheidend ist jedoch das neurologische Ergebnis: 91% unserer Patienten wiesen ein gutes neurologisches Outcome auf. Nach wie vor schwierig ist die Versorgung traumatologischer Reanimationen, die trotz maximaler Therapie eine infauste Prognose aufwiesen., The prehospital resuscitation study in Dachau included the outcome of 539 Patients with out-of-hospital cardiac arrest, of those 405 with cardiac cause. Of these 11.6% survived till release from hospital. Compared to international studies the survival rate in the area of Dachau was in the upper level. Even though this rate is quite lower than the thinkable survival rate after out-of-hospital cardiac arrest. But most deciding is the neurological outcome: 91% of our patients had a favorable neurological outcome. Still difficult is the treatment of trauma patients with cardiac arrest, which in spite of getting maximum therapy still have a very bad prognoses.

Published
2012

10. Präklinische Reanimationen im Rettungsdienstbereich Dachau

Author

Estner, Heidi L. (Priv.-Doz. Dr.), Estner, Heidi L. (Priv.-Doz. Dr.);Neumeier, Dieter (Prof. Dr.);Kochs, Eberhard (Prof. Dr.), Günzel, Christian, Estner, Heidi L. (Priv.-Doz. Dr.), Estner, Heidi L. (Priv.-Doz. Dr.);Neumeier, Dieter (Prof. Dr.);Kochs, Eberhard (Prof. Dr.), and Günzel, Christian

Abstract

In der Dachauer Reanimationsstudie konnten in einem Zeitraum von 5 Jahren Ergebnisse von 539 Reanimations-Patienten erhoben werden, davon 405 kardialer Genese. Von diesen Patienten überlebten 11,6% bis zur Klinikentlassung. Im internationalen Vergleich lagen die Überlebensraten im Landkreis Dachau im oberen Niveau. Dennoch ist dieser Anteil deutlich niedriger, als die theoretisch denkbaren Überlebensraten nach Reanimation. Entscheidend ist jedoch das neurologische Ergebnis: 91% unserer Patienten wiesen ein gutes neurologisches Outcome auf. Nach wie vor schwierig ist die Versorgung traumatologischer Reanimationen, die trotz maximaler Therapie eine infauste Prognose aufwiesen., The prehospital resuscitation study in Dachau included the outcome of 539 Patients with out-of-hospital cardiac arrest, of those 405 with cardiac cause. Of these 11.6% survived till release from hospital. Compared to international studies the survival rate in the area of Dachau was in the upper level. Even though this rate is quite lower than the thinkable survival rate after out-of-hospital cardiac arrest. But most deciding is the neurological outcome: 91% of our patients had a favorable neurological outcome. Still difficult is the treatment of trauma patients with cardiac arrest, which in spite of getting maximum therapy still have a very bad prognoses.

Published
2012

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