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Your search keyword '"Gümüşderelioğlu, Menemşe"' showing total 548 results
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548 results on '"Gümüşderelioğlu, Menemşe"'

10. Versatile cell cultivation on injectable poly(butylene adipate‐co‐terephthalate) microcarriers: Impact of surface properties across different cell types.

Author

Koç, Sena and Gümüşderelioğlu, Menemşe

Subjects
STEM cell treatment, STEM cells, ELECTRON microscopes, SURFACE properties, FIBROBLASTS
Abstract

Injectable cell therapies offer several advantages compared with traditional open surgery, including less trauma to the patient, shorter recovery time, and lower risk of infection. However, a significant problem is the difficulty in developing effective cell delivery carriers that are cyto‐compatible and maintain cell viability both during and after injection. In the presented study, it was aimed to develop poly(butylene adipate‐co‐terephthalate) (PBAT) microcarriers using the emulsion preparation‐solvent evaporation technique. The optimized diameter of the PBAT microcarriers was determined as 104 ± 15 μm at 700 rpm and there would be no blockage after injection due to the nonswelling feature of microcarriers. Furthermore, the cellular activities of PBAT microcarriers were evaluated in static culture for 7 days using L929 mouse fibroblasts, MC3T3‐E1 mouse pre‐osteoblasts, and rat adipose‐derived mesenchymal cells (AdMSCs). 3‐[4,5‐Dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide results and Sscanning electron microscope images showed that PBAT microcarriers increased the adhesion and proliferation properties of pre‐osteoblasts and stem cells, while L929 fibroblasts formed aggregates by adhering to certain regions of the microcarrier surface and did not spread on the surface. These results emphasize that PBAT microcarriers can be used as injectable carriers, especially in stem cell therapies, but their surface properties need to be modified for some cells. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Automated Cell Viability Analysis in Tissue Scaffolds

Author

Eroğul, Osman, Irmak, Gülseren, Akşahin, Mehmet Fevzi, Erdamar, Aykut, Uyar, Tansel, Gümüşderelioğlu, Menemşe, Eroğul, Osman, Irmak, Gülseren, Akşahin, Mehmet Fevzi, Erdamar, Aykut, Uyar, Tansel, and Gümüşderelioğlu, Menemşe

Abstract

Image analysis of cell biology and tissue engineering is time-consuming and requires personal expertise. However, evalu - ation of the results may be subjective. Therefore, computer-based learning and detection applications have been rapidly developed in recent years. In this study, Confocal Laser Scanning Microscope (CLSM) images of the viable pre-osteoblastic mouse MC3T3-E1 cells in 3D bioprinted tissue scaffolds, captured from a bone tissue regeneration study, were analyzed by using image processing techniques. The aim of this study is to develop a reliable and fast algorithm for the automated analysis of live/dead assay CLSM images. Percentages of live and dead cell areas in the scaffolds were determined, and then, total cell viabilities were calculated. Furthermore, manual measurements of four different analysts were obtained to evaluate subjectivity in the analysis. The measurement variations of analysts, also known as the coefficient of variation, were determined from 13.18% to 98.34% for live cell images and from 9.75% to 126.02% for dead cell images. Therefore, an automated algorithm was developed to overcome this subjectivity. The other aim of this study is to determine the depth profile of viable cells in 3D tissue scaffolds. Consequently, cross-sectional image sets of three different types of tissue scaf - folds were analyzed.

Published
2023

30. Automated Cell Viability Analysis in Tissue Scaffolds

Author

Gümüşderelioğlu, Menemşe, Uyar, Tansel, Akşahin, Mehmet Fevzi, Erdamar, Aykut, Irmak, Gülseren, Eroğul, Osman, Gümüşderelioğlu, Menemşe, Uyar, Tansel, Akşahin, Mehmet Fevzi, Erdamar, Aykut, Irmak, Gülseren, and Eroğul, Osman

Abstract

Image analysis of cell biology and tissue engineering is time-consuming and requires personal expertise. However, evalu - ation of the results may be subjective. Therefore, computer-based learning and detection applications have been rapidly developed in recent years. In this study, Confocal Laser Scanning Microscope (CLSM) images of the viable pre-osteoblastic mouse MC3T3-E1 cells in 3D bioprinted tissue scaffolds, captured from a bone tissue regeneration study, were analyzed by using image processing techniques. The aim of this study is to develop a reliable and fast algorithm for the automated analysis of live/dead assay CLSM images. Percentages of live and dead cell areas in the scaffolds were determined, and then, total cell viabilities were calculated. Furthermore, manual measurements of four different analysts were obtained to evaluate subjectivity in the analysis. The measurement variations of analysts, also known as the coefficient of variation, were determined from 13.18% to 98.34% for live cell images and from 9.75% to 126.02% for dead cell images. Therefore, an automated algorithm was developed to overcome this subjectivity. The other aim of this study is to determine the depth profile of viable cells in 3D tissue scaffolds. Consequently, cross-sectional image sets of three different types of tissue scaf - folds were analyzed.

Published
2023

38. Enhanced Osteogenic Potential of NogginKnockout C2C12 Cells on BMP-2 Releasing Silk Scaffolds

Author

Çakmak, Anıl Sera, Fuerkaiti, Sümeyra, Karagüzel, Dilara, Karaaslan, Çağatay, and Gümüşderelioğlu, Menemşe

Abstract

The CRISPR/Cas9 mechanism offers promising therapeutic approaches for bone regeneration by stimulating or suppressing critical signaling pathways. In this study, we aimed to increase the activity of BMP-2 signaling through knockout of Noggin, thereby establishing a synergistic effect on the osteogenic activity of cells in the presence of BMP-2. Since Nogginis an antagonist expressed in skeletal tissues and binds to subunits of bone morphogenetic proteins (BMPs) to inhibit osteogenic differentiation, here Nogginexpression was knocked out using the CRISPR/Cas9 system. In accordance with this purpose, C2C12 (mouse myoblast) cells were transfected with CRISPR/Cas9 plasmids. Transfection was achieved with Lipofectamine and confirmed with intense fluorescent signals in microscopic images and deletion in target sequence in Sanger sequencing analysis. Thus, Nogginknockout cells were identified as a new cell source for tissue engineering studies. Then, the transfected cells were seeded on highly porous silk scaffolds bearing BMP-2-loaded silk nanoparticles (30 ng BMP-2/mg silk nanoparticle) in the size of 288 ± 62 nm. BMP-2 is released from the scaffolds in a controlled manner for up to 60 days. The knockout of Nogginby CRISPR/Cas9 was found to synergistically promote osteogenic differentiation in the presence of BMP-2 through increased Coll1A1and Ocnexpression and mineralization. Gene editing of Nogginand BMP-2 increased almost 2-fold Col1A1expression and almost 3-fold Ocnexpression compared to the control group. Moreover, transfected cells produced extracellular matrix (ECM) containing collagen fibers on the scaffolds and mineral-like structures were formed on the fibers. In addition, mineralization characterized by intense Alizarin red staining was detected in transfected cells cultured in the presence of BMP-2, while the other groups did not exhibit any mineralized areas. As has been demonstrated in this study, the CRISPR/Cas9 mechanism has great potential for obtaining new cell sources to be used in tissue engineering studies.

Published
2023
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