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ABSTRACT Objective This study aimed to investigate the plasma and milk pharmacokinetics, as well as the withdrawal time (WT) from milk of tolfenamic acid (2 and 4 mg/kg) following intravenous (IV) administration to eight healthy lactating Akkaraman sheep. Methods The trial was conducted in two periods in accordance with a crossover pharmacokinetic design. The concentrations of tolfenamic acid in the plasma and milk were determined using high‐pressure liquid chromatography and evaluated using non‐compartmental analysis. The WT of tolfenamic acid in milk was calculated using the WT 1.4 software. Results Compared to the 2 mg/kg dose, plasma volume of distribution at steady state (from 0.43 to 0.50 L/kg), terminal elimination half‐life (from 2.41 to 4.14 h) and dose‐normalized area under the plasma concentration–time curve (AUC0−∞, from 9.46 to 30.11 h µg/mL) increased, whereas total body clearance (from 0.21 to 0.13 L/h/kg) decreased at the 4 mg/kg dose. The peak milk concentration (Cmax) and AUC0−∞ values in milk were 0.26 µg/mL and 0.28 h µg/mL, respectively, for 2 mg/kg, and 0.43 µg/mL and 0.55 h µg/mL, respectively, for 4 mg/kg. Although the dose‐normalized Cmax of milk decreased depending on the dose, no difference was observed in dose‐normalized AUC0−∞. The AUC0−∞ milk/AUC0−∞ plasma ratio was 0.03 for 2 mg/kg and 0.02 for 4 mg/kg. The WT values calculated for milk at dosages of 2 and 4 mg/kg were 3 and 4 h, respectively. Conclusions A decrease in plasma elimination and an increase in plasma concentration of tolfenamic acid were observed depending on the dose. Tolfenamic acid lowly passed into sheep's milk at 2 and 4 mg/kg doses. This study may provide valuable information for clinicians’ decision‐making processes.

Abstract Background Stenotrophomonas maltophilia is a gram-negative bacterium that can cause hospital infections and outbreaks within hospitals. This study aimed to evaluate an outbreak of Stenotrophomonas maltophilia, caused by ready-to-use commercial syringes containing liquid lithium and heparin for arterial blood gas collection in a university hospital. Methods Upon detecting an increase in Stenotrophomonas maltophilia growth in blood cultures between 15.09.2021 and 19.11.2021, an outbreak analysis and a case-control study (52 patients for the case group, 56 patients for the control group) were performed considering risk factors for bacteremia. Samples from possible foci for bacteremia were also cultured. Growing bacteria were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The genetic linkage and clonal relationship isolates were investigated with pulsed-field gel electrophoresis (PFGE) in the reference laboratory. Results In the case-control study, the odds ratio for the central venous catheter [3.38 (95% confidence interval [CI]: 1.444, 8.705 ; p = 0.006)], for surgery [3.387 (95% confidence interval [CI]: 1.370, 8.373 ; p = 0.008)] and for arterial blood gas collection history [18.584 (95% confidence interval [CI]:4.086, 84.197; p

İnsan faaliyetlerinin yol açtığı ikli m krizi, yoksulluk gibi sorunlar ve çözüm içeren atılımlar toplumlar kadar toplumun bir parçası olan kurumların da sorumluluklarını oluşturmaya başlamıştır. Sürdürülebilirliğin kurumun tümü tarafından içselleştirilmesini sağlayacak ana kavram kurum kültürüdür. Bu nitel araştırmada iki kavramın birbiri üzerindeki rolünü ortaya koyabilmek adına sürdürülebilirlik uygulamalarının ölçülebilmesinde kullanılan De Grosbois’in (2016) kurumsal sürdürülebilirlik temaları ile kurum kültürünün ölçülebilmesine olanak sağlayan Robbins’in (1990) kurum kültürü boyutlarından (Robbins, 1990, s. 439’dan akt. Vural, 2016, s. 60) yararlanarak hazırlanmış yarı yapılandırılmış görüşme formu ile derinlemesine görüşmeler yapılmıştır. Buna göre; kurum kültürünün kurumsal sürdürülebilirlik çalışmalarını desteklediğini ortaya çıkmış, eğitim kurumlarının, kültürlerini oluşturan vizyon ve misyonlarına sürdürülebilirlikle ilgili temalar ekledikleri belirlenmiştir. Okullarda çevresel sürdürülebilirlik ve toplumsal katkı konularında çalışmalar ağırlık kazanırken, sürdürülebilirlik ilkeleri oryantasyon ve çeşitli etkinlikler, kurum içi iletişim araçları ve sosyal medya aracılığıyla yerleştirilerek yeni gelen çalışanlara aktarılmaktadır.

Abstract Background Interprofessional Education (IPE) is an educational approach that brings together students from different healthcare professions to foster collaborative learning and teamwork. Before integrating IPE into the curriculum of health preprofessional students, it is necessary to increase their readiness for IPE. Dentistry increasingly values interprofessional collaboration and teamwork for enhanced patient care and healthcare team competencies, an emphasis also echoed by recent dental education authorities. The aim of this quasi-experimental research was to assess the influence of Scenario Based Learning Peer Learning (SBPL) programme, which involved scenarios necessitating interprofessional communication, on the readiness for IPE among a cohort of undergraduate dental students studying within the framework of the European Higher Education Area (EHEA). Methods This study investigates undergraduate dental students’ readiness for IPE and the influence of SBPL programme on their readiness. Participants (n = 25) from 18 EHEA countries completed the Readiness for Interprofessional Learning Scale (RIPLS) before and after SBPL programme, held at the 70th European Dental Students’ Association (EDSA) meeting. Data were analyzed using the Wilcoxon Signed Rank Test (p = 0.05). Results After the SBPT programme, there was a statistically significant increase (p

Introduction: Intraoperative fluid management is one of the most important factors affecting optimal perfusion in the microcirculatory area in patients that undergo flap surgery. While insufficient fluid administration in the intraoperative period leads to flap complications and organ dysfunction, volume load can cause complications such as edema in the denervated flap tissue, the opening of the sutures, or fat necrosis. The Infrared Fluorescent Angiography Perfusion Evaluation Device (SPY) is one of the many noninvasive techniques that evaluate the well-being of microcirculation at the tissue level. This device monitors and scores the perfusion distribution in the flap area. This retrospective study aimed to investigate the effect of fluid resuscitation in head and neck free flap transfer surgery on flap quality and patient outcomes according to the change in SPY scores. Material and Method: This study included 39 ASA I–II patients who were aged 18–60 years and underwent simultaneous free flap reconstruction of the head and neck between 2015 and 2021. Patients’ blood pressure, body temperature, hemoglobin, pH, and lactate values were recorded at both baseline and end of the operation. Also, the SPY “Infrared Fluorescent Angiography Perfusion Evaluation Device” scores, the amount of intraoperative fluid and transfusion, bleeding and urine output, and the duration of mechanical ventilation, anesthesia and surgery, and the duration and amount of drainage, the length of stay in hospital and intensive care unit, and the presence of flap infection, detachment, necrosis and loss, and re-exploration rate were recorded for the patients. Results: The difference between the first and last measured SPY values was observed to be positively correlated with the length of stay in the hospital and intensive care unit and the duration of drainage. There was a positive correlation between the length of stay in the hospital and intensive care unit and the duration of drainage, the amount of drainage, as well as the duration of anesthesia and the duration of surgery (p < 0.001). A positive correlation was found between the amount of drainage and the amount of crystalloid solution administered (r = 0.36, p < 0.05). In patients with flap infection, the difference between SPYfirst and SPYlast, the duration of anesthesia, and the duration of surgery were significantly higher. The amount of crystalloid solution given and bleeding and the duration of anesthesia and surgery were found to be significantly higher in mechanically ventilated patients (p < 0.05). Conclusions: It has been concluded that SPY-guided fluid management can be beneficial in preventing morbidities, such as extended hospital and intensive care stay, by reducing flap infection, mechanical ventilation duration, and drainage, with early diagnosis of insufficient perfusion.

PURPOSEThe clinical management of high-risk lesions using image-guided biopsy is challenging. This study aimed to evaluate the rates at which such lesions were upgraded to malignancy and identify possible predictive factors for upgrading high-risk lesions.METHODSThis retrospective multicenter analysis included 1.343 patients diagnosed with high-risk lesions using an image-guided core needle or vacuum-assisted biopsy (VAB). Only patients managed using an excisional biopsy or with at least one year of documented radiological follow-up were included. For each, the Breast Imaging Reporting and Data System (BI-RADS) category, number of samples, needle thickness, and lesion size were correlated with malignancy upgrade rates in different histologic subtypes. Pearson’s chi-squared test, the Fisher–Freeman–Halton test, and Fisher’s exact test were used for the statistical analyses.RESULTSThe overall upgrade rate was 20.6%, with the highest rates in the subtypes of intraductal papilloma (IP) with atypia (44.7%; 55/123), followed by atypical ductal hyperplasia (ADH) (38.4%; 144/375), lobular neoplasia (LN) (12.7%; 7/55), papilloma without atypia (9.4%; 58/611), flat epithelial atypia (FEA) (8.7%; 10/114), and radial scars (RSs) (4.6%; 3/65). There was a significant relationship between the upgrade rate and BI-RADS category, number of samples, and lesion size Lesion size was the most predictive factor for an upgrade in all subtypes.CONCLUSIONADH and atypical IP showed considerable upgrade rates to malignancy, requiring surgical excision. The LN, IP without atypia, pure FEA, and RS subtypes showed lower malignancy rates when the BI-RADS category was lower and in smaller lesions that had been adequately sampled using VAB. After being discussed in a multidisciplinary meeting, these cases could be managed with follow-up instead of excision.

Penetrating thoracic trauma accounts for 20–25% of all deaths due to trauma in the first four decades of life. About 33% of deaths from thoracic trauma occur due to penetrating trauma. In an autopsy study that enrolled 1178 trauma patients, 82% of the patients with tracheobronchial injuries died at the incidence site. In another study, 30% of those who could be transferred to the hospital died. This review aimed to revisit penetrating thoracic trauma with respect to complications and the strategies for airway management. While the risk of death in injuries with a sharp object is normally 1–8%, it reaches 25–28% when the cardiac box is included, and still, most of the patients are lost before they can come to the hospital. The consequences and management of penetrating thoracic trauma are mainly dependent on the extent of the injury to internal organs, as well as on the skill of the clinicians, airway obstruction, respiratory failure, and bleeding. Chest computed tomography (CT) is better than chest radiography in diagnosing the main bronchus or lobe/segment rupture. However, with the use of multi-channel multi-detector CT, the sensitivity of CT imaging has increased to 94% in the diagnosis of tracheobronchial injuries. While standard orotracheal intubation is sufficient in 75% of the patients, flexible bronchoscopy, intubation through the open wound or tracheostomy is required for airway provision in the rest. Clinical suspicion is the first diagnostic tool in a patient with penetrating airway trauma, and early treatment with multidisciplinary teamwork is life-saving.

Introduction: The cuff of an endotracheal tube seals the airway to facilitate positive-pressure ventilation and reduce subglottic secretion aspiration. However, an increase or decrease in endotracheal tube intracuff pressure can lead to many morbidities. Objective: The main purpose of this study is to investigate the effect of different head and neck positions on endotracheal tube intracuff pressure during ear and head and neck surgeries. Methods: A total of 90 patients undergoing elective right ear (Group 1: n = 30), left ear (Group 2: n = 30) or head and neck (Group 3: n = 30) surgery were involved in the study. A standardized general anesthetic was given and cuffed endotracheal tubes by the assistance of video laryngoscope were placed in all patients. The pilot balloon of each endotracheal tube was connected to the pressure transducer and standard invasive pressure monitoring was set to measure intracuff pressure values continuously. The first intracuff pressure value was adjusted to 18.4 mmHg (25 cm H2O) at supine and neutral neck position. The patients then were given appropriate head and neck positions before related-surgery started. These positions were left rotation, right rotation and extension by under-shoulder pillow with left/right rotation for Groups 1, 2 and 3, respectively. The intracuff pressures were measured and noted after each position, at 15th, 30th, 60th, 90th minutes and before the extubation. If intracuff pressure deviated from the targeted value of 20–30 cm H2O at anytime, it was set to 25 cm H2O again. Results: The intracuff pressure values were increased from 25 to 26.73 (25–28.61) cm H2O after left neck rotation (p = 0.009) and from 25 to 27.20 (25.52–28.67) cm H2O after right neck rotation (p = 0.012) in Groups 1 and 2, respectively. In Group 3, intracuff pressure values at the neutral position, after extension by under-shoulder pillow and left or right rotation were 25, 29.41 (27.02–36.94) and 34.55 (28.43–37.31) cm H2O, respectively. There were significant differences between the neutral position and extension by under-shoulder pillow (p

Objective: Granulomatous mastitis (GM) is a challenging inflammatory disorder of the breast. In this study we aimed to present the detailed clinical and morphological features of GM cases, diagnostic clues for specific and idiopathic etiologies, the difficulties in evaluating trucut biopsies, and the results of different therapeutic approaches. Material and Method: We retrospectively analysed the clinical, radiological and morphological features of 114 GM cases diagnosed with fine needle aspiration, and trucut, incisional, and excisional biopsy. Results: The mean age was 35.8. Only eight cases were older than 45 years. Bilateral involvement was observed in 4 (3.5%) cases. The most common clinical symptoms were breast mass/abscesses, tenderness, and skin changes. Microbiological culture was positive in 4 cases for gram-positive bacteria. Only 3 cases showed a positive tuberculin/PCR test for tuberculosis. The major USG finding was a hypoechoic well-defined or ill-defined mass/abscess; MRI finding was heterogeneous non-mass contrast enhancement. Cases diagnosed with cytology (35 cases) did not have breast malignancy either in their history or clinical follow up period. Fine needle aspiration cytology materials revealed epitheloid granulomas mixed with neutrophils, lymphocytes accompanied by giant cells, and suppurative necrosis. Histopathological reevaluation of 65 trucut/incisional/ excisional biopsies revealed granuloma formation in 65 (100%), Langhans type giant cells in 59 (90.7%), microabscess formation in 41 (63%), caseous necrosis in 1 (1.5%), neutrophilic cysts in 30 (46.1%), eosinophilic infiltration in 48 (73.8%), interlobular inflammation in 14 (21.5%), fat necrosis in 5 (7.6%), ductal ectasia in 6 (9.2%), and lactational changes in 4 (6.1%) cases. Granulomas were lobulocentric in 58 cases, foreign body type/fat necrosis-related in 6 case, and periductular in 1 case. Cystic neutrophilic granulomatous mastitis was observed in one case. We also evaluated the histochemical stains of these 65 biopsies. Only one sample was positive for acido-resistant bacilli (ARB) by the EZN method and one sample was positive for gram-positive bacilli by gram stain. Conclusion: Small, superficial trucut biopsies may cause difficulties in determining the etiology and differential diagnosis of granulomatous mastitis. For optimal management and timing the appropriate therapy, the ideal biopsy procedure, special stains, and a multidisciplinary team consisting of the surgeon, pathologist, and radiologist are the most important issues.

Aim: This study was carried out to determine the perceptions of the student nurses who had nursing clinical practice in the intensive care unit about the environmental stress sources in the intensive care unit. Method: The research was done by the participation of 78 students who being educated in nursing department and had nursing clinical practice at intensive care units. To collect data, “Intensive Care Unit Sources of Environmental Stress Scale” and data collection form developed by the researchers were used. Data were analyzed with percentiles, frequency, average and standart deviation values, t test for independent groups and ANOVA tests. Results: 84.6% of the students participating in the research are between the ages of 19-23, 74.4% are female, 96.2% are single and 78.2% are in the fourth grade. 71.8% of the participants had a middle income level, 42.3% of them had nursing clinical practice in the internal medicine intensive care unit and 76.9% of them had not been in the intensive care unit before. In addition, 64.1% of the students want to work in the intensive care unit. According to the results of the study, “fear of death”, “hearing the alarm of the heart monitor goes off”, “lack of privacy”, “fear of AIDS” and “experiencing pain” are the most important sources of environmental stress for students in intensive care units. In addition, it was determined that the perception of environmental stress sources in the intensive care unit was higher among the students in the 24-27 age group, men and students who had nursing clinical practice in the surgical intensive care unit. Conclusion: This study revealed that the perception of environmental stress sources in intensive care unit of nursing students. By this way, required arrangements will be done in nursing curriculum and the students will be educated more comprehensively about intensive care units.

As the cryptocurrency trading market has grown significantly in recent years, the number of comments related to cryptocurrency has increased tremendously in social media platforms. Due to this, sentiment analysis of the cryptocurrency-related comments has become highly desirable to give a comprehensive picture of peoples’ opinions about the trend of the market. In this regard, we perform cryptocurrency-related text sentiment classification using tweets based on positive and negative sentiments. For increasing the efficacy of the sentiment analysis, we introduce a novel deep neural network hybrid architecture which is composed of an embedding layer, a convolution layer, a group-wise enhancement mechanism, a bidirectional layer, an attention mechanism, and a fully connected layer. Local features are derived using a convolution layer, and weight values associated with intuitive features are developed using the group-wise enhancement mechanism. After feeding the improved context vector to the bidirectional layer to grab global features, the attention mechanism and the fully connected layer have been employed. The experimental findings indicate that the proposed architecture outperforms the state-of-the-art architectures with an accuracy value of 93.77%.

Aim: The aim of the study is to evaluate the Distress Thermometer (DT) as a valid and reliable measurement tool that can be used to measure the patients stress because of surgery. Method: This was a methodological study conducted with 200 patients. Data were collected using the the Hospital Anxiety and Depression Scale (HADS), the Distress Thermometer (DT) and surgery related distress causes list (DCL) for preoperative patients. The validation of the Distres Thermometer was performed in conjunction with the Hospital Anxiety and Depression Scale (HADS). Results: Study results determined that the distress score average of the patients was 4.4 (SD 2.4), and the distress level of 47.5% of the patients was above this average. The sensitivity of HADS, which had a cut-off point of five or more, was 72.5% and specificity was 59.3%. The most expressed causes of distress were the development of infection after surgery. There was a moderate relation between the thermometer score and the total DCL score and HADS total score. Conclusion: The distress thermometer can be used as a valid tool in determining surgery-related anxiety. And also distress causes list can be used to determine distress factors. In this study, the HADS was used as a criterion for DT-DCL, and according to this scale, the sensitivity of the thermometer was high, and the specificity was moderate. The tool can be retested by using different distress scales.

Introduction Childhood cancer is a leading cause of death. It is unclear whether the COVID-19 pandemic has impacted childhood cancer mortality. In this study, we aimed to establish all-cause mortality rates for childhood cancers during the COVID-19 pandemic and determine the factors associated with mortality.Methods Prospective cohort study in 109 institutions in 41 countries. Inclusion criteria: children

Background: Perineal hernias are rarely seen in cats and can be caused by congenital or trauma. The urinary bladder is the most herniated organ and was being observed in prepubic, ventral perineal, umbilical or caudoventral abdominal regions. Case Description: A one-year-old female domestic short-haired cat was presented to the Animal Hospital of the Near East University Faculty of Veterinary Medicine with complaints of urinary retention as well as the inability to defecate. The patient owner reported that the cat had been struck by a car around four months previously, which resulted in a pelvic fracture. On clinical examination, a fluctuating mass was detected in the dorso-lateral region of the pelvis. After radiographic and ultrasonographic examination, the mass was diagnosed as the urinary bladder. A cystopexy operation was performed on the urinary bladder, which herniated through a muscular tear to the right dorsolateral pelvic region. Conclusion: Unusual case report of a sacral hernia resulting from a traumatic rupture of the sacral muscles together with urinary bladder retroflexion was reported in a cat.

Introduction: 18F-fluorodeoxyglucose positron emission tomography/computed tomography parameters such as; maximum standardized uptake values, standard metabolic tumor volume and otal lesion glycosis are important prognostic biomarkers in cancers. Objective: To investigate the prognostic value of these parameters in patients with head and neck cancers. Methods: We performed a retrospective study including 47 patients with head and neck cancer who underwent18F-fluorodeoxyglucose positron emission tomography/computed tomography prior to treatment. Standard metabolic tumor volume, otal lesion glycosis and standardized uptake were measured for each patient. The prognostic value of quantitative 18F-fluorodeoxyglucose positron emission tomography/computed tomography parameters and clinicopathologic variables on disease free survival and overall survival were analyzed. Results: The median (range) standard metabolic tumor volume and otal lesion glycosis and standardized uptake were 7.63 cm3 (0.6–34.3), 68.9 g (2.58–524.5 g), 13.89 (4.89–33.03 g/mL), respectively. Lymph node metastases and tumour differentiation were significant variables for disease free survival and overall survival, however, all 18F-fluorodeoxyglucose positron emission tomography/computed tomography parameters were not associated with disease- free survival and overall survival. Conclusion: Pretreatment quantities positron emission tomography parameters did not predict survival in head and neck cancer.

Synthesis of silicon oxide (SiO2) via the sol-gel method and SiO2 coating ability on to Ti6Al4V alloy were investigated, in the novel study. Ti6Al4V alloy is the most used alloy in biomedical applications due to its many advantages. However, it has a minor disadvantage like releasing ions into the human body. In this study, the substrate material Ti6Al4V alloy samples were coated using the sol-gel method with alkoxysilane solutions (tetraethyl orthosilicate, TEOS; Si(OC2H5)4 and glycidoxypropyltrimethoxysilane, GPTMS; C9H20O5Si) containing the unit of prepared Si(OR)n after the surface preparations were made. The prepared solutions were examined using thermal analysis methods and the process temperatures to be applied to the coatings were determined according to the results of the analysis. Coated samples were subjected to aging, drying, and sintering processes at specified temperatures. The morphologies and structures of the samples were investigated by using a scanning electron microscope with energy-dispersive spectroscopy (SEM-EDS) and X-ray diffraction (XRD). The sol-gel coating ability of the silicon oxide on the surface of the Ti6Al4V alloys has been discussed. The results demonstrate that cracks were observed in coating produced by TEOS precursor whereas the coatings used GPTMS precursor exhibited crack-free surface independently of dipping number. The crack formation tended to reduce with increasing dipping number of coatings produced by TEOS precursor. Distances between coating islets gradually became smaller as the number of dippings increased, and the continuity of the coatings increased. It has been observed that SiO2 coating layers produced with GPTMS are obtained in a more continuous and crack-free form than the layers produced with TEOS.

Objectives Paediatric cancer is a leading cause of death for children. Children in low-income and middle-income countries (LMICs) were four times more likely to die than children in high-income countries (HICs). This study aimed to test the hypothesis that the COVID-19 pandemic had affected the delivery of healthcare services worldwide, and exacerbated the disparity in paediatric cancer outcomes between LMICs and HICs.Design A multicentre, international, collaborative cohort study.Setting 91 hospitals and cancer centres in 39 countries providing cancer treatment to paediatric patients between March and December 2020.Participants Patients were included if they were under the age of 18 years, and newly diagnosed with or undergoing active cancer treatment for Acute lymphoblastic leukaemia, non-Hodgkin’s lymphoma, Hodgkin lymphoma, Wilms’ tumour, sarcoma, retinoblastoma, gliomas, medulloblastomas or neuroblastomas, in keeping with the WHO Global Initiative for Childhood Cancer.Main outcome measure All-cause mortality at 30 days and 90 days.Results 1660 patients were recruited. 219 children had changes to their treatment due to the pandemic. Patients in LMICs were primarily affected (n=182/219, 83.1%). Relative to patients with paediatric cancer in HICs, patients with paediatric cancer in LMICs had 12.1 (95% CI 2.93 to 50.3) and 7.9 (95% CI 3.2 to 19.7) times the odds of death at 30 days and 90 days, respectively, after presentation during the COVID-19 pandemic (p

Transmissible venereal tumor (TVT) is a transmissible cancer that typically affects the external genital organs in canines. It can spread to other parts of the body via auto- and hetero-transplantation in a way that neoplastic cells are inoculated into the degraded mucosa or skin during the interaction of the animals, such as licking and sniffing, or less commonly via hematogenous or lymphatic routes. Here, we presented the clinical, cytological, and histopathological characteristics of two rare cases of TVT that developed ocular involvement and were associated with a poor prognosis. Fine needle aspiration biopsy was the initial method of choice in the diagnosis which is rapid, minimally invasive, and effective. It was diagnostic in one case and aided in the diagnosis in the other. The latter was considered a malignant round cell tumor in the cytological examination that needed further clinical and histopathological examination for definitive diagnosis. TVT is a round cell tumor that should be considered in the differential diagnosis of ocular lesions in canines.

Aluminum metal matrix composites were used in various fields such as aircraft, underwater, automobile, aerospace. Several fabrication techniques were used for the production of these composites. Among the various methods, the powder metallurgy route provides dimensional accuracy and high production rates. In this paper, metal matrix composites that include 7075 aluminum alloy chips and Al2O3 particles were produced by using a hot pressing. The effect of reinforcement amount and T6 heat treatment on the mechanical and wear properties of the composites were investigated. Al2O3 particles were added by weight of 5, 10 and 15 % and the composites were produced by hot pressing. T6 heat treatment consisted of a solution treatment at 480°C for 2 h and artificially aging process at 120°C for 24 h. Wear resistance of the composites was determined by a ball-on-disc dry sliding wear test. Brinell Hardness of the composites tended to increase with the increasing Al2O3 reinforcement up to 10 wt.% and with T6 treating. Particle reinforcement improved the wear resistance up to 10 wt.% Al2O3. In addition, volumetric material loss tended to decrease after the aging process.

Objective:The aim of this study is to investigate the efficiency of a first-line molecular genetic evaluation approach, in children with deafness.Methods:Patients who were found to have sensorineural hearing loss by age-appropriate audiological tests were selected for the molecular genetic evaluation. The molecular genetic evaluation was carried out with GJB2 gene sequence analysis and mtDNA m.1555A>G mutation Restriction Fragment Length Polymorphism (RFLP) analysis. Additionally, in a small group of patients, hearing loss Multiplex Ligation-dependent Probe Amplification (MLPA) analysis was done out to identify the possible role of copy number changes.Results:In this Turkish cohort, which included 104 index patients and 78 relatives, 33 (31.7%) had Pathogenic/Likely Pathogenic variants. One or more GJB2 sequence variants were identified in 46 (44.1%) of the 104 index patients. The homozygous c.35delG mutation by itself explained the etiology in 24% of our ARSNHL group. In one (5%) of the 20 patients of MLPA group, a hemizygous deletion in POU3F4 gene was detected.Conclusion:In our Turkish cohort, we applied a first-line molecular genetic evaluation approach using GJB2 gene sequence analysis and mtDNA m.1555A>G RFLP analysis. This approach revealed the genetic etiology of 44.1% of our index patients. Additionaly, the results of hearing loss MLPA analysis revealed the limited role of copy number changes in this patient group. Furthermore, with a detailed genotype-phenotype association workup, 2 rare cases of Deafness with Palmoplantar Hyperkeratosis and Keratitis-Ichthyosis-Deafness syndrome were reported.

Polonya'da, İkinci Dünya Savaşı'ndan önce yaklaşık üç milyon Yahudi yaşamaktaydı. Bu sayı 1939 yılında Nazi Almanya’sının Polonya'yı işgal etmesinden sonra sistemli olarak azalmıştı. Naziler, işgali altında bulundurdukları Polonya'da Yahudilere karşı olumsuz bir imaj oluşturarak, Polonya halkının Yahudilere yardım etmesini önlemeyi, aynı zamanda Polonyalıların Yahudilerin saklandıkları yerleri ihbar etmelerini amaçlamıştır. Bu çalışmada Nazi propagandasının Polonya'da Yahudilere dinine karşı nefret söylemi inşasında posterleri nasıl ve ne şekilde kullandıkları incelenmiştir. Bu amaçla çalışmada Nazi propagandası tarafından Polonya'da basılan ve günümüzde Amerika Birleşik Devletleri Holokost Anıt Müzesi'nde yer alan beş antisemitist propaganda posteri nefret söylemi bağlamında incelenmiştir. Çalışmada nitel araştırma yöntemlerinden göstergebilimsel analiz yöntemi kullanılmıştır. Çalışma kapsamında belirlenen Nazi propaganda posterleri, Fransız dilbilimci Roland Barthes'ın göstergebilim anlayışı ışığında analiz edilmiştir. Elde edilen bulgular kapsamında, Nazi propaganda posterlerinde Yahudilerin yalnızca insanlığın kötülüğü için çalıştığına ve Hıristiyanlık dini için büyük bir tehlike oluşturduğuna yönelik mitler inşa edildiği sonucuna ulaşılmıştır.

**** Note that the pagination of this article has been adjusted due to resolving a production error in another article in this issue.***

The purpose of this study is to find out the narcissism levels of the students in Faculty of Sports Sciences and to compare these in terms of some demographic variables. The sample of the study consists of a total of 180 students (66 females, 114 males) studying in three different departments of faculty of sports sciences (Physical Training and Sports Education, Sports Management and Coaching Education) who agreed to participate in the study voluntarily. In the study, Narcissistic Personality Inventory was used as data collection tool. The normality assumption tests of the data were conducted with Kolmogorov-Smirnov Test. Since the data did not meet the parametric test assumptions, nonparametric tests “’Mann-Whitney U” and “Kruskal Wallis” were used in order to find out the differences between personality traits and narcissism. Significant differences were found between age and the sub dimensions of exhibitionism and entitlement, between year of study and entitlement, and between being elite and exhibitionism and exploitativeness (p lt;0.05). In addition, branches and all sub dimensions were compared and no significant difference was found in any of the sub-dimensions (p gt;0.05). No significant difference was also found between total narcissism scores and the variables. As a conclusion, statistically significant difference was found between students’ narcissistic personality traits and the variables of age, year of study and being elite. Based on these results, the fact that the students show narcissistic personality traits although they are still students shows that it is necessary to take the necessary steps before these attitudes and perceptions reach harmful extents and continue increasingly in their occupations.

Background Dentistry has undergone an evolution in endodontics practice caused by the advancement of rotary techniques for root canal preparation and their subsequent incorporation into the teaching of dentistry undergraduates. This research aimed to evaluate the shaping ability of third-year dental students as their first experience in rotary instrumentation using ProTaper Universal (PTU) and ProTaper Next (PTN) (Dentsply Maillefer) rotary instruments in simulated curved canals. Methods Forty students instrumented 200 simulated canals with a 40° curvature in resin blocks according to the manufacturer’s instructions with PTU and 39 students and 195 canals with PTN files. The canals were prepared at a speed of 300 rpm using a 16:1 reduction hand-piece powered by an electric motor (Xsmart; Dentsply Maillefer). The final apical preparation was set to F2 for the PTU and X2 for the PTN group. The change in canal curvature was evaluated based on Schneider technique using the AutoCAD 2007 software on post-digital photographs. The incidence of instrument fracture and deformation, the incidence of ledge, the change in working length (WL), and the working time were noted. The data were analyzed with Student’s t-test and Chi-Square test at a significance level of 0.05 using SPSS. Results PTN maintained the original canal curvature better, resulting in fewer fractures and ledges, and shaped the canals faster than the PTU (P 0.05). The change in WL after instrumentation was 0.97 mm ± 0.95 mm in PTU and 0.96 mm ± 0.80 mm in PTN (p

Background Since the introduction of nickel–titanium (Ni–Ti) instruments to dentistry, a wide variety of Ni–Ti instruments have become commercially available. These Ni–Ti instruments are expensive, which limits their usage in developing countries and forces practitioners to use instruments repeatedly. Another problem is the possible prion cross-contamination associated with the multiple usage of endodontic instruments. In addition, the use of these instruments requires new skills and experience. In this article, the shaping capacities of two conventional rotary file systems, ProFile 25/0.06 and ProTaper F2, were reviewed and compared with the Reciproc single-file system. Methods A total of 45 simulated canals with 40° curvature, in clear resin blocks, were prepared using conventional rotary systems consisting of ProFile orifice shaping (OS) #3 and final flaring #25/.06, Reciproc R25, and ProTaper shaping file SX and finishing file F2. Pre-and post-instrumentation images were analyzed at ten different levels, using AutoCAD 2007 software. The measurement positions were defined in 1-mm intervals: positions 0–3 established the apical part, positions 4–6 constituted the middle part, and positions 7–10 established the coronal part of the canal. The amount of removed resin, the transportation, instrumentation time, change in working length (WL), instrumentation fractures, and the presence of ledge were evaluated. Data were analyzed using ANOVA, Kruskal–Wallis and independent t-test (p

Amaç: Bu çalışmada meme kanseri ile ilişkili lenfödem’i (MKİL) olan hastaların klinik özelliklerini tanımlamak, MKİL’in el kas gücü ve fonksiyonlarına etkisini araştırmak amaçlanmıştır.Gereç ve Yöntem: Çalışmaya onkolojik rehabilitasyon polikliniğine başvuran MKİL’i olan sağ eli dominant kadınlar dahil edildi. Çalışma prospektif olarak yürütüldü. Tüm hastaların klinik ve demografik bilgileri not edildi, fizik muayeneleri yapıldı. Omuz ağrısı varlığı sorgulandı. Elin kavrama gücünü belirlemek amacıyla Jamar el dinamometresi, parmak ucu kavramalarını değerlendirmek icin pinçmetre kullanıldı. Semmes-Weinstein monofilaman testi ile duyusal kayıplar, el volümetresi ile el volümleri değerlendirildi. Bulgular: Çalışmaya yaş ortalaması 54.5±11.4 (ortalama±SS) olan 32 kadın hasta dahil edildi. Hastaların tamamına aksiller diseksiyon yapılmıştı. Hastaların %62,5’i post- operatif dönemde radyoterapi, %96,9’u kemoterapi almıştı. Hastaların %78,1’i etkilenen tarafta omuz ağrısı olduğunu bildirdi. Sağ tarafı etkilenmiş grupta ve sol tarafı etkilenmiş grupta, sağ el ve sol elin kaba kavrama güçleri, el becerileri, duyu kaybı ve volümleri arasında anlamlı fark saptanmadı. Sol tarafı etkilenmiş grupta sağ elin parmak ucu kavrama gücü ise soldan daha fazlaydı.Sonuç: MKİL’i olan hastalarda aksiler diseksiyon yapılmış olması, post-operatif dönemde kemoterapi ve radyoterapi almış olmak, obezite ve omuz ağrısı sıktır. MKİL’li hastaların değerlendirilmesi ayrıntılı ve çok yönlü olarak yapılmalı ve omuz ağrısının sorgulanması ve el değerlendirmesini de içermelidir.

The purpose of this study was to determine the attitudes of students who had a class on disabled people and those who did not. The students who participated in the study were studying at the Faculty of Sports Sciences of Ondokuz Mayıs University during the academic year 2013-2014. Disabled people generally encounter negative attitudes. Despite all the precautions taken, being disabled is perceived as an “excluded, unaccepted and despised” concept. This behavior causes a negative attitude towards disabled people. The students' attitudes towards disabled people were analyzed in terms of demographic features. 203 (57 female and 146 male) students studying at Ondokuz Mayıs University, Yaşar Doğu Faculty of Sports Sciences participated in the study. The data was collected through a “Demographic Information Form” developed by the researcher and “Attitude Scale for Disabled People” developed by Prime Ministry Department of the Administration of the Disabled. The level of significance for all statistical procedures was taken as 0.05. All the statistical analyses were made through SPSS package program. The total average score taken by the students on Attitude Scale for Disabled People was 170.93 (n=203, Min: 122, Max:207, SD:20.18). A significant difference was found between the attitude score means of students who took classes about disabled people and those who did not, in terms of gender (p lt;0.01). The results of the study showed that the students who took lessons about disabled people had positive attitude towards disabled people. The effect of gender on the attitude towards disabled people is a result which is consistent with the literature. Thus, perceptions of the society towards disabled people should change, administrative and legal precautions should be taken to prevent the exclusion of disabled people and their families from the society and societal responsibilities should be fulfilled.

Abstract The mitotic spindle is a complex macromolecular machine that coordinates accurate chromosome segregation. The spindle accomplishes its function using forces generated by microtubules (MTs) and multiple molecular motors, but how these forces are integrated remains unclear, since the temporal activation profiles and the mechanical characteristics of the relevant motors are largely unknown. Here, we developed a computational search algorithm that uses experimental measurements to ‘reverse engineer’ molecular mechanical machines. Our algorithm uses measurements of length time series for wild‐type and experimentally perturbed spindles to identify mechanistic models for coordination of the mitotic force generators in Drosophila embryo spindles. The search eliminated thousands of possible models and identified six distinct strategies for MT–motor integration that agree with available data. Many features of these six predicted strategies are conserved, including a persistent kinesin‐5‐driven sliding filament mechanism combined with the anaphase B‐specific inhibition of a kinesin‐13 MT depolymerase on spindle poles. Such conserved features allow predictions of force–velocity characteristics and activation–deactivation profiles of key mitotic motors. Identified differences among the six predicted strategies regarding the mechanisms of prometaphase and anaphase spindle elongation suggest future experiments.

Anaphase B spindle elongation is characterized by the sliding apart of overlapping antiparallel interpolar (ip) microtubules (MTs) as the two opposite spindle poles separate, pulling along disjoined sister chromatids, thereby contributing to chromosome segregation and the propagation of all cellular life. The major biochemical “modules” that cooperate to mediate pole–pole separation include: (i) midzone pushing or (ii) braking by MT crosslinkers, such as kinesin-5 motors, which facilitate or restrict the outward sliding of antiparallel interpolar MTs (ipMTs); (iii) cortical pulling by disassembling astral MTs (aMTs) and/or dynein motors that pull aMTs outwards; (iv) ipMT plus end dynamics, notably net polymerization; and (v) ipMT minus end depolymerization manifest as poleward flux. The differential combination of these modules in different cell types produces diversity in the anaphase B mechanism. Combinations of antagonist modules can create a force balance that maintains the dynamic pre-anaphase B spindle at constant length. Tipping such a force balance at anaphase B onset can initiate and control the rate of spindle elongation. The activities of the basic motor filament components of the anaphase B machinery are controlled by a network of non-motor MT-associated proteins (MAPs), for example the key MT cross-linker, Ase1p/PRC1, and various cell-cycle kinases, phosphatases, and proteases. This review focuses on the molecular mechanisms of anaphase B spindle elongation in eukaryotic cells and briefly mentions bacterial DNA segregation systems that operate by spindle elongation.

Nonsteroidal anti-inflammatory drugs are frequently prescribed drug group in human and veterinary medicine. However, diclofenac, a traditional nonsteroidal anti-inflammatory drug, related to cardiotoxicity is reported, and blood cardiac damage markers may increase within the first hours after damage. The aim of the current research was to determine the effect of diclofenac on the blood cardiac damage markers. Single dose of diclofenac (2.5 mg/kg, IM) was injected to 6 rams. Blood samples were collected in before (0 hour, control) and 6 hours after injection. Specific (troponin I, and creatine kinase-MB) and nonspecific (lactate dehydrogenase, aspartate aminotransferase) blood cardiac damage marker concentrations, routine biochemical (hepatic damage, renal damage, lipid metabolism, glucose, and phosphorus) parameters, and hemogram values were measured. Diclofenac increased (P

Bu araştırmada inci balığının Alburnus orontis, Sauvage 1882 et kompozisyonu ve mevsimsel değişimleri incelenmiştir. Bu amaçla her mevsim 20 adet birey kullanılmıştır. İnci balığının canlı ağırlık ortalaması 21.004±8.072 g ve ortalama et verimi % 58.39±0.55 olarak belirlenmiştir. En yüksek ham protein değeri % 19.53±0.07 ile yazın, lipit % 6.67±0.04 ile kışın, kuru madde % 57.37±0.07 ile yazın, ham kül ise % 6.37±0.24 ile kışın belirlenmiştir

Aim: The objective of this study was to determine the effects of enrofloxacin (ENR), flunixin meglumine (FM) and dexamethasone (DEX) on kinetic values of cytokines, adenosine deaminase (ADA) and 13,14-dihydro-15-keto-prostaglandin F2a (PGM) in lipopolysaccharide-induced endotoxemia. Materials and Methods: Rats were divided into seven groups. To induce endotoxemia, lipopolysaccharide (LPS) was injected into all groups, including the positive control. The six other groups received the following drugs: ENR, FM, low-dose DEX, high-dose DEX, ENR + FM + low-dose DEX and ENR + FM + high-dose DEX. After the treatments, blood samples were collected at 0, 1, 2, 4, 6, 8, 12, 24, and 48 hours. Serum tumor necrosis factor alpha (TNFα), interleukin- 6 (IL-6), interleukin-10 (IL-10), ADA and plasma PGM levels were determined by ELISA. Area under the concentration time curve (AUC0-48), maximal concentration in plasma or serum (Cmax) and time to reach (tmax) values were determined by pharmacokinetic computer program. Results: ENR increased (p0-48 of PGM and decreased (p0-48 of IL-6, IL-10 and ADA, while FM decreased (p0-48 of IL-6 and ADA compared to LPS group. DEX alone and combined administrations caused the lower AUC0-48 of all values (p

Toxoplasma gondii is an apicomplexan parasite infecting all mammals including humans and causes toxoplasmosis. There is no vaccine available for humans and thus vaccine development efforts continue using novel antigens and/or vaccine platforms. Since our previous microarray screening study showed that ROP6 is a suitable antigen to be used in vaccine studies, in this study, we aimed to design an optimized mRNA construct encoding the ROP6 protein and then demonstrate its efficiency and immunogenicity using in vitro methods. For this, we constructed a pT7CFE1-Chis/ROP6 vector encoding optimized ROP6 mRNA containing EMCV 5'UTR with IRES and a 20 nucleotides fragment from alpha globin 3' UTR. Then, we generated the optimized ROP6 mRNAs with anti-reverse cap analogue (ARCA) and approximately 150 nucleotide long poly-A tail. Next, HEK293T cells were transfected with the optimized ROP6 mRNAs to show recombinant ROP6 protein expression capability. Moreover, we expressed in vitro recombinant ROP6 protein in HeLa cell lysate using the pT7CFE1-Chis/ROP6 vector to reveal the immunogenicity of recombinant ROP6 protein using sera samples collected from mice infected with PRU strain of T. gondii. The IFA and Western blot results showed that the optimized ROP6 mRNAs successfully expressed the recombinant ROP6 protein in HEK293T cells. Moreover the recombinant ROP6 protein expressed in HeLa cell lysate strongly reacted with sera samples collected from mice. The absorbance difference detected among positive and negative mice serum samples analyzed was statistically significant, indicating that the recombinant ROP6 protein was immunogenic (P = 0.0003). In conclusion, this study demonstrated that the optimized ROP6 mRNAs can be used in the development of mRNA vaccines against toxoplasmosis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Purpose: To evaluate the structural and microvascular changes in the retina and choroid after strabismus surgery., Methods: The study included 44 eyes from 44 patients who underwent strabismus surgery between August 2020 and December 2021. Among these patients, 9 (20%) underwent horizontal one-muscle surgery, 27 (61%) underwent horizontal two-muscle surgery, and 8 (18%) underwent inferior oblique muscle anteriorization surgery. Optical coherence tomography angiography scans were conducted preoperatively and at 1 day, 1 week, 1 month, and 3 months postoperatively., Results: The outer retinal blood flow showed a significant increase at 1 day postoperatively in horizontal two-muscle and inferior oblique muscle anteriorization surgery, whereas no significant difference was observed between periods in horizontal one-muscle surgery ( P = .007). Choriocapillaris blood flow exhibited a significant increase at 1 day and 1 month postoperatively compared to the preoperative period ( P < .001). Whole image superficial capillary plexus vascular density was significantly lower at 1 day postoperatively than preoperatively and 1 and 3 months postoperatively ( P < .001). Whole image deep capillary plexus vascular density was significantly lower at 1 day than at 1 month postoperatively ( P = .002)., Conclusions: Following strabismus surgery, temporary changes in the hemodynamic microcirculation in the retina and choroid are observed. A transient decrease in retinal artery blood supply may be noticed, possibly attributed to the reduced anterior segment blood supply. [ J Pediatr Ophthalmol Strabismus . 2024;61(6):397-403.] .

Toxoplasma gondii is an obligate intracellular parasite that can infect a variety of mammals including humans and causes toxoplasmosis. Unfortunately, a protective and safe vaccine against toxoplasmosis hasn't been developed yet. In this study, we developed a DNA vaccine encoding the SRS13 protein and immunized BALB/c mice thrice with pVAX1-SRS13 through the intramuscular route (IM) or intradermally using an electroporation device (ID + EP). The immunogenicity of pVAX1-SRS13 was analyzed by ELISA, Western blot, cytokine ELISA, and flow cytometry. The protective efficacy of the pVAX1-SRS13 was investigated by challenging mice orally with T. gondii PRU strain tissue cysts. The results revealed that pVAX1-SRS13 administered through IM or ID + EP routes induced high level of anti-SRS13 IgG antibody responses (P = 0.0037 and P < 0.0001). The IFN-γ level elicited by the pVAX1-SRS13 (ID + EP) was significantly higher compared to the control group (P = 0.00159). In mice administered with pVAX1-SRS13 (ID + EP), CD8 + cells secreting IFN-γ was significantly higher compared to pVAX1-SRS13 (IM) (P = 0.0035) and the control group (P = 0.0068). Mice vaccinated with the SRS13 DNA vaccine did not induce significant IL-4 level. Moreover, a significant reduction in the number of tissue cysts and the load of T. gondii DNA was detected in brains of mice administered with pVAX1-SRS13 through ID + EP and IM routes compared to controls. In conclusion, the SRS13 DNA vaccine was found to be highly immunogenic and confers strong protection against chronic toxoplasmosis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Objectives: The objective of the study was to evaluate the prevalence and potential risk factors associated with retinopathy of prematurity (ROP) in very low birth weight (BW) ROP patients stratified by different BW categories., Methods: This retrospective cohort study examined very low BW patients (≤1500 g) treated at a neonatal intensive care unit and subsequently assessed for ROP at the outpatient clinic. Data on gestational age (GA), BW, ROP severity, treatments, and outcomes were collected following international ROP classification criteria. Patients with type 1 ROP and aggressive ROP received treatment. Patients were categorized based on 250 g BW intervals, and ROP frequency and treatment rates were assessed by GA., Results: In this study, 116 patients, comprising 60.3% of females and 39.7% of males, were analyzed. The GA ranged from 23 to 34 weeks, with a mean of 30.03±2.64 weeks, while the mean BW was 1108±275 g, ranging from 370 g to 1490 g. ROP was present in 49.1% of patients and 19.8% required treatment. Lower BW and GA were significantly associated with ROP (p<0.05). ROP incidence and treatment rates varied across BW groups., Conclusion: ROP diagnosis and treatment rates have risen over time, reflecting improvements in intensive care. Categorizing premature infants based on BW facilitates the development of screening criteria tailored to neonatal intensive care units and aids in predicting ROP diagnosis and treatment rates., Competing Interests: Conflict of Interest: None declared.

Introduction:  Anastomotic stricture (AS) is the second most common complication after esophageal atresia (EA) repair. We aimed to evaluate the data in the Turkish Esophageal Atresia Registry to determine the risk factors for AS development after EA repair in a large national cohort of patients., Methods:  The data between 2015 and 2021 were evaluated. Patients were enrolled into two groups according to the occurrence of AS. Patients with AS (AS group) and without AS (non-AS group) were compared according to demographic and operative features, postoperative intubation status, and postoperative complications, such as anastomotic leaks, fistula recanalization, and the presence of gastroesophageal reflux (GER). A multivariable logistic regression analysis was performed to define the risk factors for the development of AS after EA repair., Results:  Among the 713 cases, 144 patients (20.19%) were enrolled into the AS group and 569 (79.81%) in the non-AS group. The multivariable logistic regression showed that, being a term baby (odds ratio [OR]: 1.706; p  = 0.006), having a birth weight over 2,500 g (OR: 1.72; p  = 0.006), presence of GER (OR: 5.267; p  < 0.001), or having a recurrent tracheoesophageal fistula (TEF, OR: 4.363; p  = 0.006) were the risk factors for the development of AS., Conclusions:  The results of our national registry demonstrate that 20% of EA patients developed AS within their first year of life. In patients with early primary anastomosis, birth weight greater than 2,500 g and presence of GER were risk factors for developing AS. When patients with delayed anastomosis were included, in addition to the previous risk factors, being a term baby, and having recurrent TEF also became risk factors., Level of Evidence:  III., Competing Interests: None declared., (Thieme. All rights reserved.)

Severe acute respiratory syndrome coronavirus 2 had devastating consequences for human health. Despite the introduction of several vaccines, COVID-19 continues to pose a serious health risk due to emerging variants of concern. DNA vaccines gained importance during the pandemic due to their advantages such as induction of both arms of immune response, rapid development, stability, and safety profiles. Here, we report the immunogenicity and protective efficacy of a DNA vaccine encoding spike protein with D614G mutation (named pcoSpikeD614G) and define a large-scale production process. According to the in vitro studies, pcoSpikeD614G expressed abundant spike protein in HEK293T cells. After the administration of pcoSpikeD614G to BALB/c mice through intramuscular (IM) route and intradermal route using an electroporation device (ID + EP), it induced high level of anti-S1 IgG and neutralizing antibodies (P < 0.0001), strong Th1-biased immune response as shown by IgG2a polarization (P < 0.01), increase in IFN-γ levels (P < 0.01), and increment in the ratio of IFN-γ secreting CD4 + (3.78-10.19%) and CD8 + (5.24-12.51%) T cells. Challenging K18-hACE2 transgenic mice showed that pcoSpikeD614G administered through IM and ID + EP routes conferred 90-100% protection and there was no sign of pneumonia. Subsequently, pcoSpikeD614G was evaluated as a promising DNA vaccine candidate and scale-up studies were performed. Accordingly, a large-scale production process was described, including a 36 h fermentation process of E. coli DH5α cells containing pcoSpikeD614G resulting in a wet cell weight of 242 g/L and a three-step chromatography for purification of the pcoSpikeD614G DNA vaccine., (© 2024. The Author(s).)