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255 results on '"Göhde W"'

8. Tumorforschung I

Author

Steinhoff, H.-J., Clasen, B. P. E., Janssen, Th., Axhausen, M., Herold, M., Tausch-Treml, R., Reiman, V., Holtmann, S., Vogl, Th., Weber, H., Schroeder, H.-G., Kleinsasser, O., Wolf, J., Laskawi, R., Brauneis, J., Schröder, M., Göhde, W., Maier, H., Stevens, F. S., Weidauer, H., Altmannsberger, M., Kroschel, S., Mischke, D., Lörz, M., Metz, C., Wendler, E., Meyer-Breiting, E., Wustrow, T. P. U., Kabelitz, D., Welkoborsky, H. J., Sorger, K., Knuth, A., Wustrow, J., Hansmann, M.-L., Deitmer, T., Freytag, G., Swoboda, H., Neumann, H., Cartellieri, M., Matschke, R. G., Heilmann-Jedamzik, C., Jedamzik, J., Plath, P., Kumazawa, H., Koldovsky, P., Kürten, Ch., Vosteen, K.-H., Fleischer, Konrad, editor, and Wigand, Malte Erik, editor

Published
1988
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9. Cell Synchronization in Solid Tumors

Author

Schumann, J., Ehring, F., Göhde, W., Allfrey, V. G., editor, Allgöwer, M., editor, Bauer, K. H., editor, Berenblum, I., editor, Bergel, F., editor, Bernard, J., editor, Bernhard, W., editor, Blokhin, N. N., editor, Bock, H. E., editor, Braun, W., editor, Bucalossi, P., editor, Chaklin, A. V., editor, Chorazy, M., editor, Cunningham, G. J., editor, Dargent, M., editor, Della Porta, G., editor, Denoix, P., editor, Dulbecco, R., editor, Eagle, H., editor, Eker, R., editor, Good, R. A., editor, Grabar, P., editor, Hamperl, H., editor, Harris, R. J. C., editor, Hecker, E., editor, Herbeuval, R., editor, Higginson, J., editor, Hueper, W. C., editor, Isliker, H., editor, Kieler, J., editor, Klein, G., editor, Koprowski, H., editor, Koss, L. G., editor, Martz, G., editor, Mathé, G., editor, Mühlbock, O., editor, Nakahara, W., editor, Old, L. J., editor, Potter, V. R., editor, Sabin, A. B., editor, Sachs, L., editor, Saxén, E. A., editor, Schmidt, C. G., editor, Spiegelman, S., editor, Szybalski, W., editor, Tagnon, H., editor, Taylor, R. M., editor, Tissières, A., editor, Uehlinger, E., editor, Wissler, R. W., editor, Rentchnick, P., editor, Grundmann, Ekkehard, editor, and Gross, Rudolf W. J., editor

Published
1975
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18. Proliferationskinetik. Cytophotometrie

Author

Boll, Irene, Fliedner, T. M., Hoelzer, D., Harriss, E. B., Theml, H., Schick, P., Trepel, F., Swan Lee, Be, Kaboth, W., Begemann, H., Bremer, K., Weissenfels, I., Koss, G., Herm, B., Nitzel, H., Büchner, Th., Dittrich, W., Göhde, W., Krygier-Stojałowska, Aleksandra, Urasiński, Ignacy, Müller, D., Reichert, E., D. Lang, H., Simon, A., Benohr, H. Ch., Schaefer, U. W., Dicke, K. A., Gross, Rudolf, editor, and van de Loo, Jurgen, editor

Published
1972
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28. Flow Cytometry and Sizing for Routine Andrological Analysis

Author

SPANO, M., primary, CALUGI, A., additional, CAPUANO, V., additional, VITA, R., additional, GÖHDE, W., additional, HACKER-KLOM, U., additional, MAISTRO, A., additional, MAURO, F., additional, OTTO, F., additional, PACCHIEROTTI, F., additional, ROCCHINI, P., additional, SCHUMANN, J., additional, TEODORI, L., additional, and VIZZONE, A., additional

Published
2009
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49. Affordable CD4+ T-cell counts on ‘single-platform’ flow cytometers I. Primary CD4 gating.

Author

Janossy, G., Jani, I., and Göhde, W.

Subjects
FLOW cytometry, CD4 antigen
Abstract

Here, we demonstrate the flow cytometric concept of ‘primary CD4 gating’ utilizing three different CD4 monoclonal antibodies (mAbs) conjugated with five different fluorochromes. CD4+ lymphocytes were defined by an autogate in a single histogram of CD4 fluorescence intensity (FI) (y-axis) vs. side light scatter (x-axis). A wide range of absolute counts for > 600 individuals, including HIV+ patients, were compared with those obtained by ‘state-of-the-art’ single-platform flow cytometers such as the volumetric Ortho CytoronAbsolute and the Becton Dickinson FACSCalibur using TruCount beads. The correlation between CD4 counts obtained with primary CD4 gating and the full test panel on the Ortho Cytoron was excellent (R2 = 0·999). Bland–Altman statistics showed a mean difference of -2 cells/mm3 [confidence interval (CI) 95% = -3 to -1; limits of agreement -27 to +23]. In addition to absolute CD4 counts, CD4% values and CD4/CD8 ratios are also frequently requested. To obtain these, lymphocytes need to be counted using scatter gates, and a second tube stained with a CD8 mAb to count CD8++ lymphocytes can be incorporated. We conclude that primary CD4 gating on single-platform volumetric flow cytometers is one of the most economical and flexible technologies for routine cost-conscious service work, particularly during the follow-up of patients undergoing anti-HIV therapy and/or vaccination in the developing world. [ABSTRACT FROM AUTHOR]

Published
2000
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50. Andrology. DNA flow cytometry of human semen.

Author

Hacker-Klom, U.B., Göhde, W., Nieschlag, E., and Behre, H.M.

Abstract

The aim of this study was the evaluation of DNA flow cytometry for the analysis of male infertility. 171 ejaculates from 155 patients with fertility problems were analysed by flow cytometry and by conventional microscopical procedures. Using flow cytometry, it was possible to determine the relative proportions of the various cell populations: mature haploid and abnormal diploid mature spermatozoa, cellular fragments, immature germ cells (haploid round spermatids, diploid cells, S phase and 4C cells), and of leukocytes as indicators of infection. A linear association was observed between sperm concentration in semen as quantified by light microscopy and by flow cytometry, even with fewer than 20×106 spermatozoa/ml. Eight classes of histograms, each with differing fractions of spermatozoa and other particles, were obtained and correlated with the results of the spermiograms. During the 10 year follow-up, the two patient groups with a low sperm concentration or a high concentration of cellular debris exhibited significantly impaired fertility. The two patient groups with ≥5% diploid spermatozoa and with malcondensed sperm chromatin were also subfertile. No ovulatory disorders were revealed in the 155 female partners. DNA flow cytometry thus provides an additional dimension to semen analysis not easily gained by other methods and has the advantage of being rapidly performed and interpreted. We therefore recommend application of this technique in the diagnosis of male infertility. [ABSTRACT FROM PUBLISHER]

Published
1999
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