1. Ethanol, at physiological concentrations, affects ethylene sensing in tomato germinating seeds and seedlings
- Author
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Emma Carrie, Rasha Althiab Almasaud, Guilhem Desbrosses, Brad M. Binder, Christian Chervin, Yi Chen, Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Institut national de recherche pour l'agriculture, l'alimentation et l'environnement - INRAE (FRANCE), Montpellier SupAgro (FRANCE), Ningbo University - NBU (CHINA), Université de Montpellier (FRANCE), University of Tennessee - UT (USA), Génomique et Biotechnologie des Fruits - GBF (Castanet-Tolosan, France), Génomique et Biotechnologie des Fruits (GBF), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ningbo University (NBU), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), The University of Tennessee [Knoxville], US National Science Foundation (NSF) for grant MCB-1517032, and ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011)
- Subjects
0106 biological sciences ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Ethylene ,[SDV]Life Sciences [q-bio] ,Mutant ,Germination ,Biotechnologies ,Plant Science ,Biology ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Solanum lycopersicum ,Plant Growth Regulators ,Arabidopsis ,Signal perception ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Tomato seed ,030304 developmental biology ,0303 health sciences ,Ethanol ,Wild type ,food and beverages ,General Medicine ,Ethylenes ,Seed germination ,biology.organism_classification ,Horticulture ,chemistry ,Seedlings ,Seeds ,Imbibition ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
International audience; Ethanol is known to accumulate in various plant organs under various environmental conditions. However, there are very scarce data about ethanol sensing by plants. We observed that ethanol accumulates up to 3.5 mM during tomato seed imbibition, particularly when seeds were stacked. Stacked seeds germinated less than spread out seeds suggesting ethanol inhibits germination. In support of this, exogenous ethanol at physiological concentrations, ranging from 1 to 10 mM, inhibited germination of wild type tomato seeds. However, the germination pattern over the whole ethanol concentration range tested was modified in an ethylene insensitive mutant, never-ripe (nr). The effects of exogenous ethanol were not linked to differences in ethylene production by imbibed seeds. But, we observed that exogenous ethanol at a concentration as low as 0.01 mM down regulated the expression of some ethylene receptors. Moreover, the triple response induced by ethylene in tomato seedlings was partially alleviated by 1 mM ethanol. Similar observations were made on Arabidopsis seeds. These results show there are interactions between ethylene sensing and ethanol in plants.
- Published
- 2020