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The visualization of the cervix and subsequent manipulations during transcervical applications in sheep might require a degree of straining reflexes while using the Collin Speculum. Therefore, this study aimed to develop and to evaluate the feasibility of a new alternative speculum design for non-surgical assisted reproductive procedures in small ruminants. Collin speculum (CS) and the new "Onder speculum" (OS) were used to assess cervical entrance in 30 ewes. The OS efficiency for a successful cervical retraction was also evaluated. Data showed that the cervical entrance was visible in 8 out of the 30 females with CS, while it was observed in all the animals when using OS (P<0.001). The latter was successful in the retraction of the cervical entrance in 10 randomly selected animals out of the total. To conclude, the newly developed speculum effectively supported the implementation of non-surgical assisted reproductive procedures in small ruminants and was also considered to have the potential to facilitate non-invasive reproductive techniques in these species. [ABSTRACT FROM AUTHOR]

Copyright of Kocatepe Veterinary Journal / Kocatepe Veteriner Dergisi is the property of Afyon Kocatepe University, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim of the present study was to evaluate the effects of insulin supplemented extender on bull semen at a post-thaw stage. Semen samples were collected four times from a Zavot bull which is endangered species in Türkiye. Semen samples were diluted with Tris based extender supplemented with 10 IU or 15 IU of insulin and control. Motility and plasma membrane integrity were evaluated by a phase-contrast microscope. Acrosome integrity (Fitc-Peanut Agglutinin), mitochondrial membrane potential (Rhodamine 123), nitric oxide level (4,5-Diaminofluorescein-2/diacetate) were evaluated with dual staining propidium iodide using flow cytometry. Motility and plasma membrane functional integrity were better preserved compared to control group (P<0.05). Acrosome integrity results were statistically similar between control and 10 IU insulin groups (P>0.05), but increased insulin negatively affected acrosome integrity (p<0.05). Mitochondrial membrane potential was found to be higher when compared all cells in insulin containing groups compared to control (P<0.05). However, results were found similar in all groups with PI negative/mitochondrial membrane potential positive (P>0.05). There were not any significant differences among groups in terms of nitric oxide level (P>0.05). In conclusion, it was thought that insulin involved in energy metabolism and improved preservation of bull semen. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Research in Veterinary Medicine is the property of Uludag Universitesi Veteriner Fakultesi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)