30 results on '"Fuster-Tormo, F"'
Search Results
2. Topic: AS07-Singular Entities/Subtypes/AS07e-Chronic myelomonocytic leukemia and overlap syndromes (MDS/MPN): GENOMIC AND TRANSCRIPTOMIC CHARACTERIZATION OF MYELODYSPLASTIC SYNDROMES/MYELOPROLIFERATIVE NEOPLASMS
- Author
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Acha, P., primary, Ezponda, T., additional, Vilas-Zornoza, A., additional, Xicoy, B., additional, Palomo, L., additional, Fuster-Tormo, F., additional, Manzanares, A., additional, Zufiaurre, N. Berastegui, additional, Zamora, L., additional, Jerez, A., additional, López-Cadenas, F., additional, Diez-Campelo, M., additional, Bonadies, N., additional, Cervera-Zamora, J., additional, Mascaró, M., additional, Montoro, M.J., additional, Hernández, F., additional, Raya, J.M., additional, Ancín, I., additional, Vahí, M., additional, Garrastazul, M.P., additional, Hermosín-Ramos, M.L., additional, Mallo, M., additional, Grau, J., additional, Marcé, S., additional, Cabezón, M., additional, Prosper, F., additional, and Solé, F., additional
- Published
- 2023
- Full Text
- View/download PDF
3. P118 - Topic: AS07-Singular Entities/Subtypes/AS07e-Chronic myelomonocytic leukemia and overlap syndromes (MDS/MPN): GENOMIC AND TRANSCRIPTOMIC CHARACTERIZATION OF MYELODYSPLASTIC SYNDROMES/MYELOPROLIFERATIVE NEOPLASMS
- Author
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Acha, P., Ezponda, T., Vilas-Zornoza, A., Xicoy, B., Palomo, L., Fuster-Tormo, F., Manzanares, A., Zufiaurre, N. Berastegui, Zamora, L., Jerez, A., López-Cadenas, F., Diez-Campelo, M., Bonadies, N., Cervera-Zamora, J., Mascaró, M., Montoro, M.J., Hernández, F., Raya, J.M., Ancín, I., Vahí, M., Garrastazul, M.P., Hermosín-Ramos, M.L., Mallo, M., Grau, J., Marcé, S., Cabezón, M., Prosper, F., and Solé, F.
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- 2023
- Full Text
- View/download PDF
4. P052 - Topic: AS04-MDS Biology and Pathogenesis/AS04d-Somatic mutations: TP53 ALLELIC STATE DID NOT INFLUENCE THE PROGNOSIS IN MYELODYSPLASTIC SYNDROMES (MDS) WITH 5Q DELETION
- Author
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Montoro, M.J., Palomo, L., Haferlach, C., Fuster-Tormo, F., Meggendorfer, M., Xicoy, B., Schulz, F., Della Porta, M., Gonzalez, T., López-Cadenas, F., Acha, P., Diez-Campelo, M., Jerez, A., Such, E., Bernal, T., Santini, V., Platzbecker, U., Germing, U., Solé, F., Haferlach, T., and Valcárcel, D.
- Published
- 2023
- Full Text
- View/download PDF
5. t(1;19)(q23;p13) TCF3-PBX1 May Not Be an Intermediate-Risk Subtype in Adult B-Cell Precursor Acute Lymphoblastic Leukemia Patients Treated With MRD-Oriented Protocols from the PETHEMA Group
- Author
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Ribera, J, Morgades, M, Granada, I, Torrent, A, Zamora, L, Gonzalez, T, Ciudad, J, Barrena, S, Such, E, Avetisyan, G, Calasanz, MJ, Genesca, E, Gonzalez-Gil, C, Fuster-Tormo, F, Mercadal, S, Maluquer, C, Coll, R, Gonzalez-Campos, J, Tormo, M, Garcia-Cadenas, I, Nomdedeu, J, Gil, C, Cervera, M, Escoda, L, Montesinos, P, Barba, P, Esteve, J, Diaz-Beya, M, Martinez-Sanchez, P, Martinez-Lopez, J, Novo, A, Queipo, MP, Bermudez, A, Bergua, J, Olave, MT, de Rueda, B, Artola, MT, Hernandez-Rivas, JM, Orfao, A, and Ribera, JM
- Subjects
TCF3-PBX1 ,PETHEMA ,outcome ,MRD-oriented trials ,acute lymphoblastic leukemia - Published
- 2021
6. Adverse prognostic impact of complex karyotype (>= 3 cytogenetic alterations) in adult T-cell acute lymphoblastic leukemia (T-ALL)
- Author
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Genesca, E, Morgades, M, Gonzalez-Gil, C, Fuster-Tormo, F, Haferlach, C, Meggendorfer, M, Montesinos, P, Barba, P, Gil, C, Coll, R, Moreno, MJ, Martinez-Carballeira, D, Garcia-Cadenas, I, Vives, S, Ribera, J, Gonzalez-Campos, J, Diaz-Beya, M, Mercadal, S, Artola, MT, Cladera, A, Tormo, M, Bermudez, A, Vall-llovera, F, Martinez-Sanchez, P, Amigo, ML, Monsalvo, S, Novo, A, Cervera, M, Garcia-Guinon, A, Ciudad, J, Cervera, J, Hernandez-Rivas, JM, Granada, I, Haferlach, T, Orfao, A, Sole, F, and Ribera, JM
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Cytogenetics ,NGS ,Adult T-ALL ,Therapy ,Prognosis - Abstract
The potential prognostic value of conventional karyotyping in adult T-cell acute lymphoblastic leukemia (T-ALL) remains an open question. We hypothesized that a modified cytogenetic classification, based on the number and type of cytogenetic abnormalities, would allow the identification of high-risk adult T-ALL patients. Complex karyotype defined by the presence of >3 cytogenetic alterations identified T-ALL patients with poor prognosis in this study. Karyotypes with >3 abnormalities accounted for 16 % (22/139) of all evaluable karyotypes, corre-sponding to the largest poor prognosis cytogenetic subgroup of T-ALL identified so far. Patients carrying kar-yotypes with >3 cytogenetic alterations showed a significantly inferior response to therapy, and a poor outcome in terms of event-free survival (EFS), overall survival (OS) and cumulative incidence of relapse (CIR), inde-pendently of other baseline characteristics and the end-induction minimal residual disease (MRD) level. Addi-tional molecular analyses of patients carrying >3 cytogenetic alterations showed a unique molecular profile that could contribute to understand the underlying molecular mechanisms of resistance and to evaluate novel tar-geted therapies (e.g. IL7R directed) with potential impact on outcome of adult T-ALL patients.
- Published
- 2021
7. Complex Karyotype with >= 3 Cytogenetic Alterations is a New Marker of Worse Prognosis in Adult T-Cell Acute Lymphoblastic Leukemia (T-ALL)
- Author
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Genesca, E, Morgades, M, Gonzalez-Gil, C, Fuster-Tormo, F, Haferlach, C, Meggendorfer, M, Montesinos, P, Barba, P, Gil, C, Coll, R, Moreno, MJ, Martinez-Carballeira, D, Garcia-Cadenas, I, Vives, S, Ribera, J, Gonzalez-Campos, J, Diaz-Beya, M, Mercadal, S, Artola, T, Cladera, A, Tormo, M, Bermudez, A, Vall-Llovera, F, Martinez-Sanchez, P, Amigo, ML, Monsalvo, S, Novo, A, Cervera, M, Garcia-Grinon, A, Ciudad, J, Cervera, J, Hernandez-Rivas, JM, Granada, I, Haferlach, T, Orfao, A, Francesc Solé, and Ribera, JM
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treatment ,karyotype1 NGS ,next-generation sequencing ,acute lymphoblastic leukemia ,prognosis ,adult T-ALL - Published
- 2020
8. Molecular landscape and clonal architecture of adult myelodysplastic/myeloproliferative neoplasms
- Author
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Palomo L, Meggendorfer M, Hutter S, Twardziok S, Ademà V, Fuhrmann I, Fuster-Tormo F, Xicoy B, Zamora L, Acha P, Kerr CM, Kern W, Maciejewski JP, Solé F, Haferlach C, and Haferlach T
- Subjects
hemic and lymphatic diseases ,food and beverages - Abstract
More than 90% of patients with myelodysplastic/myeloproliferative neoplasms (MDSs/MPNs) harbor somatic mutations in myeloid-related genes, but still, current diagnostic criteria do not include molecular data. We performed genome-wide sequencing techniques to characterize the mutational landscape of a large and clinically well-characterized cohort including 367 adults with MDS/MPN subtypes, including chronic myelomonocytic leukemia (CMML; n = 119), atypical chronic myeloid leukemia (aCML; n = 71), MDS/MPN with ring sideroblasts and thrombocytosis (MDS/MPN-RS-T; n = 71), and MDS/MPN unclassifiable (MDS/MPN-U; n = 106). A total of 30 genes were recurrently mutated in =3% of the cohort. Distribution of recurrently mutated genes and clonal architecture differed among MDS/MPN subtypes. Statistical analysis revealed significant correlations between recurrently mutated genes, as well as genotype-phenotype associations. We identified specific gene combinations that were associated with distinct MDS/MPN subtypes and that were mutually exclusive with most of the other MDSs/MPNs (eg, TET2-SRSF2 in CMML, ASXL1-SETBP1 in aCML, and SF3B1-JAK2 in MDS/MPN-RS-T). Patients with MDS/MPN-U were the most heterogeneous and displayed different molecular profiles that mimicked the ones observed in other MDS/MPN subtypes and that had an impact on the outcome of the patients. Specific gene mutations also had an impact on the outcome of the different MDS/MPN subtypes, which may be relevant for clinical decision-making. Overall, the results of this study help to elucidate the heterogeneity found in these neoplasms, which can be of use in the clinical setting of MDS/MPN.
- Published
- 2020
9. TARGETED DEEP SEQUENCING OF PERIPHERAL CD34+CELLS CAN REPRODUCE BONE MARROWMOLECULAR PROFILE IN MDS PATIENTS
- Author
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Acha, P, Martin, R, Palomo, L, Ganster, C, Dierks, S, Mallo, M, Adema, V, Fuster-Tormo, F, Gomez-Marzo, P, De Haro, N, Jimenez-Garcia, F, Solanes, N, Zamora, L, Xicoy, B, Kominowski, A, Stromburg, M, Brockmann, A, Truemper, L, Francesc Solé, and Haase, D
- Published
- 2017
10. Inspecting Targeted Deep Sequencing of Whole Genome Amplified DNA Versus Fresh DNA for Somatic Mutation Detection: A Genetic Study in Myelodysplastic Syndrome Patients
- Author
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Palomo, L, Fuster-Tormo, F, Alvira, D, Adema, V, Armengol, MP, Gomez-Marzo, P, de Haro, N, Mallo, M, Xicoy, B, Zamora, L, and Francesc Solé
- Subjects
DNA banking ,whole genome amplification ,next-generation sequencing ,targeted deep sequencing ,myelodysplastic syndromes - Abstract
Whole genome amplification (WGA) has become an invaluable method for preserving limited samples of precious stock material and has been used during the past years as an alternative tool to increase the amount of DNA before library preparation for next-generation sequencing. Myelodysplastic syndromes (MDS) are a group of clonal hematopoietic stem cell disorders characterized by presenting somatic mutations in several myeloid-related genes. In this work, targeted deep sequencing has been performed on four paired fresh DNA and WGA DNA samples from bone marrow of MDS patients, to assess the feasibility of using WGA DNA for detecting somatic mutations. The results of this study highlighted that, in general, the sequencing and alignment statistics of fresh DNA and WGA DNA samples were similar. However, after variant calling and when considering variants detected at all frequencies, there was a high level of discordance between fresh DNA and WGA DNA (overall, a higher number of variants was detected in WGA DNA). After proper filtering, a total of three somatic mutations were detected in the cohort. All somatic mutations detected in fresh DNA were also identified in WGA DNA and validated by whole exome sequencing.
- Published
- 2017
11. Analysis of MDS patients by Targeted Deep Sequencing revealed comparable results for circulating CD34(+) blood cells and bone marrow aspirate
- Author
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Martin, R, Acha, P, Ganster, C, Palomo, L, Dierks, S, Mallo, M, Adema, V, Fuster-Tormo, F, Gomez-Marzo, P, De Haro, N, Solanes, N, Zamora, L, Xicoy, B, Kominowski, A, Stromburg, M, Brockmann, A, Francesc Solé, and Haase, D
- Published
- 2017
12. Targeted Deep Sequencing of Peripheral CD34+ Cells can Reproduce Bone Marrow Molecular Profile in MDS Patients
- Author
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Acha, P., primary, Martin, R., additional, Palomo, L., additional, Ganster, C., additional, Dierks, S., additional, Mallo, M., additional, Ademà, V., additional, Fuster-Tormo, F., additional, Gómez-Marzo, P., additional, De Haro, N., additional, Jiménez-García, F., additional, Solanes, N., additional, Zamora, L., additional, Xicoy, B., additional, Kominowski, A., additional, Stromburg, M., additional, Brockmann, A., additional, Truemper, L., additional, Sole, F., additional, and Haase, D., additional
- Published
- 2017
- Full Text
- View/download PDF
13. Spanish Guidelines for the use of Targeted Deep Sequencing in MDS and CMML
- Author
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Palomo, L., primary, Ibáñez, M., additional, Abáigar, M., additional, Vázquez, I., additional, Álvarez, S., additional, Cabezón, M., additional, Tazón-Vega, B., additional, Acha, P., additional, Benito, R., additional, Cervera, J., additional, Cigudosa, J.C., additional, Fuster-Tormo, F., additional, Hernández Sánchez, J.M., additional, Larrayoz, M.J., additional, Valcárcel, D., additional, Zamora, L., additional, Sanz, G., additional, Calasanz, M.J., additional, Solé, F., additional, and Such, E., additional
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- 2017
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- View/download PDF
14. 267 - Targeted Deep Sequencing of Peripheral CD34+ Cells can Reproduce Bone Marrow Molecular Profile in MDS Patients
- Author
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Acha, P., Martin, R., Palomo, L., Ganster, C., Dierks, S., Mallo, M., Ademà, V., Fuster-Tormo, F., Gómez-Marzo, P., De Haro, N., Jiménez-García, F., Solanes, N., Zamora, L., Xicoy, B., Kominowski, A., Stromburg, M., Brockmann, A., Truemper, L., Sole, F., and Haase, D.
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- 2017
- Full Text
- View/download PDF
15. 26 - Spanish Guidelines for the use of Targeted Deep Sequencing in MDS and CMML
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Palomo, L., Ibáñez, M., Abáigar, M., Vázquez, I., Álvarez, S., Cabezón, M., Tazón-Vega, B., Acha, P., Benito, R., Cervera, J., Cigudosa, J.C., Fuster-Tormo, F., Hernández Sánchez, J.M., Larrayoz, M.J., Valcárcel, D., Zamora, L., Sanz, G., Calasanz, M.J., Solé, F., and Such, E.
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- 2017
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16. Genomics Improves Risk Stratification of Adults with T-Cell Acute Lymphoblastic Leukemia Patients Enrolled in Measurable Residual Disease-Oriented Trials
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Celia González-Gil, Mireia Morgades, Thaysa Lopes, Francisco Fuster-Tormo, Jesús García-Chica, Ran Zhao, Pau Montesinos, Anna Torrent, Marina Diaz-Beya, Rosa Coll, Lourdes Hermosín, Santiago Mercadal, José González-Campos, Lurdes Zamora, Teresa Artola, Ferran Vall-Llovera, Mar Tormo, Cristina Gil-Cortés, Pere Barba, Andrés Novo, Jordi Ribera, Teresa Bernal, Paula López De Ugarriza, María-Paz Queipo, Pilar Martínez-Sánchez, Alicia Giménez, Teresa González-Martínez, Antonia Cladera, José Cervera, Rosa Fernández-Martín, María Ángeles Ardaiz, María Jesús Vidal, Ángela Baena, Nuria López-Bigas, Anna Bigas, Jaroslaw Maciejewski, Alberto Orfao, Josep Maria Ribera, Eulalia Genescà, Institut Català de la Salut, [González-Gil C, Lopes T, Fuster-Tormo F, García-Chica J] Institut d’Investigació contra la Leucemia Josep Carreras (IJC), Campus ICO-Germans Trias i Pujol, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Morgades M] Departament d’Hematologia Clínica, ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Zhao R] Department of Quantitative Health Sciences and Leukemia Program, Department of Hematology and Medical Oncology, Cleveland Clinic, Cleveland, OH, USA. [Barba P] Servei d’Hematologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain, and Vall d'Hebron Barcelona Hospital Campus
- Subjects
Genòmica ,Natural Science Disciplines::Biological Science Disciplines::Biology::Computational Biology::Genomics [DISCIPLINES AND OCCUPATIONS] ,neoplasias::neoplasias por tipo histológico::leucemia::leucemia linfoide::leucemia-linfoma linfoblástico de células precursoras::leucemia-linfoma linfoblástico de células T precursoras [ENFERMEDADES] ,Cèl·lules T ,Otros calificadores::Otros calificadores::/genética [Otros calificadores] ,Leucèmia limfoblàstica - Aspectes genètics ,disciplinas de las ciencias naturales::disciplinas de las ciencias biológicas::biología::biología computacional::genómica [DISCIPLINAS Y OCUPACIONES] ,Other subheadings::Other subheadings::/genetics [Other subheadings] ,Hematology ,Neoplasms::Neoplasms by Histologic Type::Leukemia::Leukemia, Lymphoid::Precursor Cell Lymphoblastic Leukemia-Lymphoma::Precursor T-Cell Lymphoblastic Leukemia-Lymphoma [DISEASES] - Abstract
Genomics; T-cell acute lymphoblastic leukemia Genòmica; leucèmia limfoblàstica aguda de cèl·lules T Genómica; Leucemia linfoblástica aguda de células T Genetic information has been crucial to understand the pathogenesis of T-cell acute lymphoblastic leukemia (T-ALL) at diagnosis and at relapse, but still nowadays has a limited value in a clinical context. Few genetic markers are associated with the outcome of T-ALL patients, independently of measurable residual disease (MRD) status after therapy. In addition, the prognostic relevance of genetic features may be modulated by the specific treatment used. We analyzed the genetic profile of 145 T-ALL patients by targeted deep sequencing. Genomic information was integrated with the clinicalbiological and survival data of a subset of 116 adult patients enrolled in two consecutive MRD-oriented trials of the Spanish PETHEMA (Programa Español de Tratamientos en Hematología) group. Genetic analysis revealed a mutational profile defined by DNMT3A/ N/KRAS/ MSH2/ U2AF1 gene mutations that identified refractory/resistant patients. Mutations in the DMNT3A gene were also found in the non-leukemic cell fraction of patients with T-ALL, revealing a possible mutational-driven clonal hematopoiesis event to prime T-ALL in elderly. The prognostic impact of this adverse genetic profile was independent of MRD status on day +35 of induction therapy. The combined worse-outcome genetic signature and MRD on day +35 allowed risk stratification of T-ALL into standard or high-risk groups with significantly different 5- year overall survival (OS) of 52% (95% confidence interval: 37-67) and 17% (95% confidence interval: 1-33), respectively. These results confirm the relevance of the tumor genetic profile in predicting patient outcome in adult T-ALL and highlight the need for novel gene-targeted chemotherapeutic schedules to improve the OS of poor-prognosis T-ALL patients. This project was supported by the AECC (GC16173697BIGA); ISCIII (PI19/01828 and PI19/01183), co-funded by ERDF/ESF, "A way to make Europe"/"Investing in your future", CERCA/Generalitat de Catalunya SGR 2017 288 (GRC)/ “La Caixa”. C Gon-zález-Gil was supported by AGAUR grant (ref: 2020 FI_B2 00210).
- Published
- 2022
17. Adverse prognostic impact of complex karyotype (≥3 cytogenetic alterations) in adult T-cell acute lymphoblastic leukemia (T-ALL)
- Author
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José Cervera, Daniel Martínez-Carballeira, Silvia Monsalvo, Ferran Vall-Llovera, Francesc Solé, Alberto Orfao, Pilar Martínez-Sánchez, Mar Tormo, Eulàlia Genescà, Pere Barba, Torsten Haferlach, Andrés Novo, Rosa Coll, Jordi Ribera, Mireia Morgades, Jesús María Hernández-Rivas, Isabel Granada, Francisco Fuster-Tormo, Celia González-Gil, Cristina Gil, Antonia Cladera, Marta Cervera, Claudia Haferlach, Juana Ciudad, Marina Díaz-Beyá, Antonio Garcia-Guiñon, Santiago Mercadal, José González-Campos, Arancha Bermúdez, Pau Montesinos, María-Teresa Artola, Susana Vives, Manja Meggendorfer, María-Luz Amigo, Josep-Maria Ribera, María-José Moreno, Irene García-Cadenas, Institut Català de la Salut, [Genescà E, González-Gil C, Fuster-Tormo F] Josep Carreras Leukaemia Research Institute (IJC), Campus ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona (UAB), Badalona, Spain. [Morgades M] Josep Carreras Leukaemia Research Institute (IJC), Campus ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona (UAB), Badalona, Spain. Clinical Hematology Department, ICO-Hospital Germans Trias i Pujol, Badalona, Spain. [Haferlach C, Meggendorfer M] MLL Munich Leukemia Laboratory, Munich, Germany. [Barba P] Servei d’Hematologia Clínica, Vall d'Hebron Hospital Universitari, Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus, Asociación Española Contra el Cáncer, Instituto de Salud Carlos III, Generalitat de Catalunya, and La Caixa
- Subjects
Male ,Oncology ,Cancer Research ,Neoplasm, Residual ,Leucèmia limfoblàstica - Prognosi ,Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ,Genetic Phenomena::Genetic Variation::Mutation::Chromosome Aberrations [PHENOMENA AND PROCESSES] ,0302 clinical medicine ,neoplasias::neoplasias por tipo histológico::leucemia::leucemia linfoide::leucemia-linfoma linfoblástico de células precursoras [ENFERMEDADES] ,Cumulative incidence ,Citogenètica humana ,Human cytogenetics ,Leukemia ,Leucèmia ,Karyotype ,Hematology ,Middle Aged ,Prognosis ,030220 oncology & carcinogenesis ,NGS ,Adult T-Cell Acute Lymphoblastic Leukemia ,Female ,fenómenos genéticos::variación genética::mutación::aberraciones cromosómicas [FENÓMENOS Y PROCESOS] ,Adult ,medicine.medical_specialty ,Pronòstic mèdic ,Adolescent ,Young Adult ,03 medical and health sciences ,Cytogenetics ,Internal medicine ,Complex Karyotype ,medicine ,Humans ,Diagnosis::Prognosis [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT] ,Interleukin-7 receptor ,diagnóstico::pronóstico [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS] ,Chromosome Aberrations ,Neoplasms::Neoplasms by Histologic Type::Leukemia::Leukemia, Lymphoid::Precursor Cell Lymphoblastic Leukemia-Lymphoma [DISEASES] ,business.industry ,Minimal residual disease ,Anomalies cromosòmiques ,Molecular Profile ,Adult T-ALL ,Therapy ,business ,030215 immunology - Abstract
© 2021 The Author(s)., The potential prognostic value of conventional karyotyping in adult T-cell acute lymphoblastic leukemia (T-ALL) remains an open question. We hypothesized that a modified cytogenetic classification, based on the number and type of cytogenetic abnormalities, would allow the identification of high-risk adult T-ALL patients. Complex karyotype defined by the presence of ≥3 cytogenetic alterations identified T-ALL patients with poor prognosis in this study. Karyotypes with ≥3 abnormalities accounted for 16 % (22/139) of all evaluable karyotypes, corresponding to the largest poor prognosis cytogenetic subgroup of T-ALL identified so far. Patients carrying karyotypes with ≥3 cytogenetic alterations showed a significantly inferior response to therapy, and a poor outcome in terms of event-free survival (EFS), overall survival (OS) and cumulative incidence of relapse (CIR), independently of other baseline characteristics and the end-induction minimal residual disease (MRD) level. Additional molecular analyses of patients carrying ≥3 cytogenetic alterations showed a unique molecular profile that could contribute to understand the underlying molecular mechanisms of resistance and to evaluate novel targeted therapies (e.g. IL7R directed) with potential impact on outcome of adult T-ALL patients., This project was supported by the AECC (GC16173697BIGA); ISCIII (PI19/01828) co-funded by ERDF/ESF "A way to make Europe"/ "Investing in your future", CERCA/Generalitat de Catalunya SGR 2017 288 (GRC)/ “La Caixa” P. Barba was supported by the Instituto de Salud Carlos III FIS16/01433 and PERIS 2018-2020 from Generalitat de Catalunya (BDNS357800).
- Published
- 2021
18. Contribution of copy number to improve risk stratification of adult T-cell acute lymphoblastic leukemia patients enrolled in measurable residual disease-oriented trials.
- Author
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Gonzalez-Gil C, Morgades M, Lopes T, Fuster-Tormo F, Montesinos P, Barba P, Diaz-Beya M, Hermosin L, Maluquer C, Gonzalez-Campos J, Bernal T, Arriaga MS, Zamora L, Pratcorona M, Martino R, Larrayoz MJ, Artola T, Torrent A, Vall-Llovera F, Tormo M, Gil C, Novo A, Martinez-Sanchez P, Ribera J, Queipo MP, Gonzalez-Martinez T, Cabrero M, Cladera A, Cervera J, Orfao A, Ribera JM, and Genesca E
- Abstract
Not available.
- Published
- 2024
- Full Text
- View/download PDF
19. Genomics improves risk stratification of adults with T-cell acute lymphoblastic leukemia enrolled in measurable residual disease-oriented trials.
- Author
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González-Gil C, Morgades M, Lopes T, Fuster-Tormo F, García-Chica J, Zhao R, Montesinos P, Torrent A, Diaz-Beya M, Coll R, Hermosín L, Mercadal S, González-Campos J, Zamora L, Artola T, Vall-Llovera F, Tormo M, Gil-Cortés C, Barba P, Novo A, Ribera J, Bernal T, De Ugarriza PL, Queipo MP, Martínez-Sánchez P, Giménez A, González-Martínez T, Cladera A, Cervera J, Fernández-Martín R, Ardaiz MÁ, Vidal MJ, Baena Á, López-Bigas N, Bigas A, Maciejewski J, Orfao A, Ribera JM, and Genescà E
- Subjects
- Humans, Adult, Aged, Disease-Free Survival, Prognosis, Neoplasm, Residual genetics, Genomics, T-Lymphocytes pathology, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma drug therapy, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma genetics, Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics
- Abstract
Genetic information has been crucial to understand the pathogenesis of T-cell acute lymphoblastic leukemia (T-ALL) at diagnosis and at relapse, but still nowadays has a limited value in a clinical context. Few genetic markers are associated with the outcome of T-ALL patients, independently of measurable residual disease (MRD) status after therapy. In addition, the prognostic relevance of genetic features may be modulated by the specific treatment used. We analyzed the genetic profile of 145 T-ALL patients by targeted deep sequencing. Genomic information was integrated with the clinicalbiological and survival data of a subset of 116 adult patients enrolled in two consecutive MRD-oriented trials of the Spanish PETHEMA (Programa Español de Tratamientos en Hematología) group. Genetic analysis revealed a mutational profile defined by DNMT3A/ N/KRAS/ MSH2/ U2AF1 gene mutations that identified refractory/resistant patients. Mutations in the DMNT3A gene were also found in the non-leukemic cell fraction of patients with T-ALL, revealing a possible mutational-driven clonal hematopoiesis event to prime T-ALL in elderly. The prognostic impact of this adverse genetic profile was independent of MRD status on day +35 of induction therapy. The combined worse-outcome genetic signature and MRD on day +35 allowed risk stratification of T-ALL into standard or high-risk groups with significantly different 5- year overall survival (OS) of 52% (95% confidence interval: 37-67) and 17% (95% confidence interval: 1-33), respectively. These results confirm the relevance of the tumor genetic profile in predicting patient outcome in adult T-ALL and highlight the need for novel gene-targeted chemotherapeutic schedules to improve the OS of poor-prognosis T-ALL patients.
- Published
- 2023
- Full Text
- View/download PDF
20. Mutational signatures are markers of drug sensitivity of cancer cells.
- Author
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Levatić J, Salvadores M, Fuster-Tormo F, and Supek F
- Subjects
- DNA metabolism, DNA Mutational Analysis methods, Humans, Mutation, Cell Line, Tumor drug effects, Cell Line, Tumor metabolism, Drug Resistance, Neoplasm genetics, Exome genetics, Neoplasms drug therapy, Neoplasms genetics
- Abstract
Genomic analyses have revealed mutational footprints associated with DNA maintenance gone awry, or with mutagen exposures. Because cancer therapeutics often target DNA synthesis or repair, we asked if mutational signatures make useful markers of drug sensitivity. We detect mutational signatures in cancer cell line exomes (where matched healthy tissues are not available) by adjusting for the confounding germline mutation spectra across ancestries. We identify robust associations between various mutational signatures and drug activity across cancer cell lines; these are as numerous as associations with established genetic markers such as driver gene alterations. Signatures of prior exposures to DNA damaging agents - including chemotherapy - tend to associate with drug resistance, while signatures of deficiencies in DNA repair tend to predict sensitivity towards particular therapeutics. Replication analyses across independent drug and CRISPR genetic screening data sets reveal hundreds of robust associations, which are provided as a resource for drug repurposing guided by mutational signature markers., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
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21. Adverse prognostic impact of complex karyotype (≥3 cytogenetic alterations) in adult T-cell acute lymphoblastic leukemia (T-ALL).
- Author
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Genescà E, Morgades M, González-Gil C, Fuster-Tormo F, Haferlach C, Meggendorfer M, Montesinos P, Barba P, Gil C, Coll R, Moreno MJ, Martínez-Carballeira D, García-Cadenas I, Vives S, Ribera J, González-Campos J, Díaz-Beya M, Mercadal S, Artola MT, Cladera A, Tormo M, Bermúdez A, Vall-Llovera F, Martínez-Sánchez P, Amigo ML, Monsalvo S, Novo A, Cervera M, García-Guiñon A, Ciudad J, Cervera J, Hernández-Rivas JM, Granada I, Haferlach T, Orfao A, Solé F, and Ribera JM
- Subjects
- Adolescent, Adult, Female, Humans, Karyotype, Male, Middle Aged, Neoplasm, Residual diagnosis, Neoplasm, Residual genetics, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma diagnosis, Prognosis, Young Adult, Chromosome Aberrations, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma genetics
- Abstract
The potential prognostic value of conventional karyotyping in adult T-cell acute lymphoblastic leukemia (T-ALL) remains an open question. We hypothesized that a modified cytogenetic classification, based on the number and type of cytogenetic abnormalities, would allow the identification of high-risk adult T-ALL patients. Complex karyotype defined by the presence of ≥3 cytogenetic alterations identified T-ALL patients with poor prognosis in this study. Karyotypes with ≥3 abnormalities accounted for 16 % (22/139) of all evaluable karyotypes, corresponding to the largest poor prognosis cytogenetic subgroup of T-ALL identified so far. Patients carrying karyotypes with ≥3 cytogenetic alterations showed a significantly inferior response to therapy, and a poor outcome in terms of event-free survival (EFS), overall survival (OS) and cumulative incidence of relapse (CIR), independently of other baseline characteristics and the end-induction minimal residual disease (MRD) level. Additional molecular analyses of patients carrying ≥3 cytogenetic alterations showed a unique molecular profile that could contribute to understand the underlying molecular mechanisms of resistance and to evaluate novel targeted therapies (e.g. IL7R directed) with potential impact on outcome of adult T-ALL patients., (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
22. Analysis of Intratumoral Heterogeneity in Myelodysplastic Syndromes with Isolated del(5q) Using a Single Cell Approach.
- Author
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Acha P, Palomo L, Fuster-Tormo F, Xicoy B, Mallo M, Manzanares A, Grau J, Marcé S, Granada I, Rodríguez-Luaces M, Diez-Campelo M, Zamora L, and Solé F
- Abstract
Myelodysplastic syndromes (MDS) are a heterogeneous group of hematological diseases. Among them, the most well characterized subtype is MDS with isolated chromosome 5q deletion (MDS del(5q)), which is the only one defined by a cytogenetic abnormality that makes these patients candidates to be treated with lenalidomide. During the last decade, single cell (SC) analysis has emerged as a powerful tool to decipher clonal architecture and to further understand cancer and other diseases at higher resolution level compared to bulk sequencing techniques. In this study, a SC approach was used to analyze intratumoral heterogeneity in four patients with MDS del(5q). Single CD34+CD117+CD45+CD19- bone marrow hematopoietic stem progenitor cells were isolated using the C1 system (Fluidigm) from diagnosis or before receiving any treatment and from available follow-up samples. Selected somatic alterations were further analyzed in SC by high-throughput qPCR (Biomark HD, Fluidigm) using specific TaqMan assays. A median of 175 cells per sample were analyzed. Inferred clonal architectures were relatively simple and either linear or branching. Similar to previous studies based on bulk sequencing to infer clonal architecture, we were able to observe that an ancestral event in one patient can appear as a secondary hit in another one, thus reflecting the high intratumoral heterogeneity in MDS del(5q) and the importance of patient-specific molecular characterization.
- Published
- 2021
- Full Text
- View/download PDF
23. Genetic characterization of acute myeloid leukemia patients with mutations in IDH1/2 genes.
- Author
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Acha P, Hoyos M, Pratcorona M, Fuster-Tormo F, Palomo L, Ortega E, Zamora L, Vives S, Granada I, Montoro J, Garcia A, Arnan M, Cervera M, Canet M, Gallardo D, Arenillas L, Esteve J, Baragay J, Salamero O, Motlló C, Ortín X, Sierra J, and Solé F
- Subjects
- Adult, Female, Humans, Male, Isocitrate Dehydrogenase genetics, Leukemia, Myeloid, Acute enzymology, Leukemia, Myeloid, Acute genetics, Mutation, Neoplasm Proteins genetics
- Published
- 2021
- Full Text
- View/download PDF
24. Matching cell lines with cancer type and subtype of origin via mutational, epigenomic, and transcriptomic patterns.
- Author
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Salvadores M, Fuster-Tormo F, and Supek F
- Subjects
- Cell Line, Tumor, Gene Expression Profiling, Mutation, Transcriptome, Epigenomics, Neoplasms genetics
- Abstract
Cell lines are commonly used as cancer models. The tissue of origin provides context for understanding biological mechanisms and predicting therapy response. We therefore systematically examined whether cancer cell lines exhibit features matching the presumed cancer type of origin. Gene expression and DNA methylation classifiers trained on ~9000 tumors identified 35 (of 614 examined) cell lines that better matched a different tissue or cell type than the one originally assigned. Mutational patterns further supported most reassignments. For instance, cell lines identified as originating from the skin often exhibited a UV mutational signature. We cataloged 366 "golden set" cell lines in which transcriptomic and epigenomic profiles strongly resemble the cancer type of origin, further proposing their assignments to subtypes. Accounting for the uncertain tissue of origin in cell line panels can change the interpretation of drug screening and genetic screening data, revealing previously unknown genomic determinants of sensitivity or resistance., (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
- Published
- 2020
- Full Text
- View/download PDF
25. Distinct mutational pattern of myelodysplastic syndromes with and without 5q- treated with lenalidomide.
- Author
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Adema V, Palomo L, Toma A, Kosmider O, Fuster-Tormo F, Benito R, Salgado R, Such E, Larrayoz MJ, Xicoy B, Hernandez-Sanchez JM, Maietta P, Neef A, Fontenay M, Ibañez M, Diez-Campelo M, Alvarez S, Maciejewski JP, Fenaux P, and Sole F
- Subjects
- Humans, Lenalidomide pharmacology, Mutation, Lenalidomide therapeutic use, Myelodysplastic Syndromes drug therapy
- Published
- 2020
- Full Text
- View/download PDF
26. Spanish Guidelines for the use of targeted deep sequencing in myelodysplastic syndromes and chronic myelomonocytic leukaemia.
- Author
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Palomo L, Ibáñez M, Abáigar M, Vázquez I, Álvarez S, Cabezón M, Tazón-Vega B, Rapado I, Fuster-Tormo F, Cervera J, Benito R, Larrayoz MJ, Cigudosa JC, Zamora L, Valcárcel D, Cedena MT, Acha P, Hernández-Sánchez JM, Fernández-Mercado M, Sanz G, Hernández-Rivas JM, Calasanz MJ, Solé F, and Such E
- Subjects
- Guidelines as Topic, Humans, Spain, High-Throughput Nucleotide Sequencing, Leukemia, Myelomonocytic, Chronic genetics, Myelodysplastic Syndromes genetics
- Abstract
The landscape of medical sequencing has rapidly changed with the evolution of next generation sequencing (NGS). These technologies have contributed to the molecular characterization of the myelodysplastic syndromes (MDS) and chronic myelomonocytic leukaemia (CMML), through the identification of recurrent gene mutations, which are present in >80% of patients. These mutations contribute to a better classification and risk stratification of the patients. Currently, clinical laboratories include NGS genomic analyses in their routine clinical practice, in an effort to personalize the diagnosis, prognosis and treatment of MDS and CMML. NGS technologies have reduced the cost of large-scale sequencing, but there are additional challenges involving the clinical validation of these technologies, as continuous advances are constantly being made. In this context, it is of major importance to standardize the generation, analysis, clinical interpretation and reporting of NGS data. To that end, the Spanish MDS Group (GESMD) has expanded the present set of guidelines, aiming to establish common quality standards for the adequate implementation of NGS and clinical interpretation of the results, hoping that this effort will ultimately contribute to the benefit of patients with myeloid malignancies., (© 2019 The Authors. British Journal of Haematology published by British Society for Haematology and John Wiley & Sons Ltd.)
- Published
- 2020
- Full Text
- View/download PDF
27. Diagnostic and prognostic contribution of targeted NGS in patients with triple-negative myeloproliferative neoplasms.
- Author
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Acha P, Xandri M, Fuster-Tormo F, Palomo L, Xicoy B, Cabezón M, Marcé S, Granada I, Vela D, Sagüés M, Boque C, Plensa E, Pineda A, Feliu E, Solé F, and Zamora L
- Subjects
- Adult, Aged, Aged, 80 and over, DNA (Cytosine-5-)-Methyltransferases genetics, DNA Methyltransferase 3A, DNA-Binding Proteins genetics, Dioxygenases, Exons genetics, Female, Humans, Male, Middle Aged, Phosphoproteins genetics, Primary Myelofibrosis diagnosis, Primary Myelofibrosis mortality, Prognosis, Proto-Oncogene Proteins genetics, RNA Splicing Factors genetics, Thrombocythemia, Essential diagnosis, Thrombocythemia, Essential mortality, DNA genetics, DNA Mutational Analysis methods, High-Throughput Nucleotide Sequencing, Primary Myelofibrosis genetics, Receptors, Thrombopoietin genetics, Thrombocythemia, Essential genetics
- Published
- 2019
- Full Text
- View/download PDF
28. Targeted deep sequencing of CD34+ cells from peripheral blood can reproduce bone marrow molecular profile in myelodysplastic syndromes.
- Author
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Martin R, Acha P, Ganster C, Palomo L, Dierks S, Fuster-Tormo F, Mallo M, Ademà V, Gómez-Marzo P, De Haro N, Solanes N, Zamora L, Xicoy B, Shirneshan K, Flach J, Braulke F, Schanz J, Kominowski A, Stromburg M, Brockmann A, Trümper L, Solé F, and Haase D
- Subjects
- Cytogenetic Analysis, Humans, Karyotyping, Leukocytes, Mononuclear, Myelodysplastic Syndromes pathology, Antigens, CD34 blood, Bone Marrow Cells pathology, High-Throughput Nucleotide Sequencing methods, Mutation, Myelodysplastic Syndromes genetics
- Published
- 2018
- Full Text
- View/download PDF
29. DNA methylation profile in chronic myelomonocytic leukemia associates with distinct clinical, biological and genetic features.
- Author
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Palomo L, Malinverni R, Cabezón M, Xicoy B, Arnan M, Coll R, Pomares H, García O, Fuster-Tormo F, Grau J, Feliu E, Solé F, Buschbeck M, and Zamora L
- Subjects
- Aged, Case-Control Studies, DNA-Binding Proteins genetics, Dioxygenases, Disease-Free Survival, Epigenesis, Genetic, Female, Gene Expression Regulation, Leukemic, Humans, Kaplan-Meier Estimate, Leukemia, Myelomonocytic, Chronic etiology, Male, Mutation, Oligonucleotide Array Sequence Analysis, Prognosis, Proto-Oncogene Proteins genetics, DNA Methylation, Leukemia, Myelomonocytic, Chronic genetics, Leukemia, Myelomonocytic, Chronic mortality
- Abstract
Chromosomal abnormalities are detected in 20-30% of patients with chronic myelomonocytic leukemia (CMML) and correlate with prognosis. On the mutation level, disruptive alterations are particularly frequent in chromatin regulatory genes. However, little is known about the consequential alterations in the epigenetic marking of the genome. Here, we report the analysis of genomic DNA methylation patterns of 64 CMML patients and 10 healthy controls, using a DNA methylation microarray focused on promoter regions. Differential methylation analysis between patients and controls allowed us to identify abnormalities in DNA methylation, including hypermethylation of specific genes and large genome regions with aberrant DNA methylation. Unsupervised hierarchical cluster analysis identified two main clusters that associated with the clinical, biological, and genetic features of patients. Group 1 was enriched in patients with adverse clinical and biological characteristics and poorer overall and progression-free survival. In addition, significant differences in DNA methylation were observed between patients with low risk and intermediate/high risk karyotypes and between TET2 mutant and wild type patients. Taken together, our results demonstrate that altered DNA methylation patterns reflect the CMML disease state and allow to identify patient groups with distinct clinical features.
- Published
- 2018
- Full Text
- View/download PDF
30. Inspecting Targeted Deep Sequencing of Whole Genome Amplified DNA Versus Fresh DNA for Somatic Mutation Detection: A Genetic Study in Myelodysplastic Syndrome Patients.
- Author
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Palomo L, Fuster-Tormo F, Alvira D, Ademà V, Armengol MP, Gómez-Marzo P, de Haro N, Mallo M, Xicoy B, Zamora L, and Solé F
- Subjects
- DNA chemistry, DNA genetics, DNA Mutational Analysis standards, Humans, DNA standards, DNA Mutational Analysis methods, High-Throughput Nucleotide Sequencing standards, Mutation genetics, Myelodysplastic Syndromes genetics, Sequence Analysis, DNA standards
- Abstract
Whole genome amplification (WGA) has become an invaluable method for preserving limited samples of precious stock material and has been used during the past years as an alternative tool to increase the amount of DNA before library preparation for next-generation sequencing. Myelodysplastic syndromes (MDS) are a group of clonal hematopoietic stem cell disorders characterized by presenting somatic mutations in several myeloid-related genes. In this work, targeted deep sequencing has been performed on four paired fresh DNA and WGA DNA samples from bone marrow of MDS patients, to assess the feasibility of using WGA DNA for detecting somatic mutations. The results of this study highlighted that, in general, the sequencing and alignment statistics of fresh DNA and WGA DNA samples were similar. However, after variant calling and when considering variants detected at all frequencies, there was a high level of discordance between fresh DNA and WGA DNA (overall, a higher number of variants was detected in WGA DNA). After proper filtering, a total of three somatic mutations were detected in the cohort. All somatic mutations detected in fresh DNA were also identified in WGA DNA and validated by whole exome sequencing.
- Published
- 2017
- Full Text
- View/download PDF
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