88 results on '"Furuse C"'
Search Results
2. Angiogenesis in salivary carcinomas with and without myoepithelial differentiation
- Author
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Costa, A. F., Demasi, A. P. D., Bonfitto, V. L. L., Bonfitto, J. F. L., Furuse, C., Araújo, V. C., Metze, K., and Altemani, A.
- Published
- 2008
- Full Text
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3. Peroxiredoxin I is differentially expressed in multiple myelomas and in plasmablastic lymphomas
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Demasi, A PD, Magalhães, M HC, Furuse, C, Araújo, N S, Junqueira, J LC, and Araújo, V C
- Published
- 2008
4. Substance P regulates the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in cultured human gingival fibroblasts
- Author
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Cury, P. R., Canavez, F., de Araújo, V. C., Furuse, C., and de Araújo, N. S.
- Published
- 2008
5. STAT3 EXPRESSION AND ACTIVATION IS DYSREGULATED IN ACTINIC CHEILITIS
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Araujo, V. C., Cury, P. R., Furuse, C., and Araújo, N. S.
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- 2007
6. CORTISOL AFFECTS MRNA EXPRESSION FOR MATRIX METALLOPROTEINASES AND TISSUE INHIBITORS OF METALLOPROTEINASES IN HUMAN GINGIVAL FIBROBLASTS
- Author
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Cury, P. R., Araújo, V. C., Leite, K. R.M., Furuse, C., and Araújo, N. S.
- Published
- 2007
7. Alveolar Bone Microstructural Modifications Induced by Weight Gain in Rats
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Leite, Fábio R., Almeida, A. F., Furuse, C., Gustavo Nascimento, Costa, S. F., Okamoto, R., and Rodrigo Lopez
- Published
- 2017
8. Mini-implants for Orthodontic Anchorage: Surface Analysis after Redrilling and Sterilization – An in vitro Study
- Author
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Gross, JM, primary, Nascimento, GG, additional, Araújo, VC, additional, Bönecker, MJS, additional, and Furuse, C, additional
- Published
- 2016
- Full Text
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9. P-094. Contribution to the study of lower lip carcinoma
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Biazolla, E. R. [UNESP], Furuse, C. F. [UNESP], Pelissaro, G. S. [UNESP], Collado, F. U. [UNESP], Sandefeld, M. L.M.M. [UNESP], and Universidade Estadual Paulista (UNESP)
- Abstract
Made available in DSpace on 2022-04-28T20:08:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2001-12-01 Aim: Carcinoma of the lower lip may be diagnosed early, because it occupies an anatomical area that is easily accessible and visible. However, in literature there are still few articles about both epidemiological and clinical studies with regard to it. The aim of this work was to analyze variables that could influence the prognosis, incidence, complication arising from treatment and the interval of time between the appearance of the lesion and the diagnosis. Methods: An analysis was made of 31 cases treated at the Oral Cancer Center of the Paulista State University, Unesp, Aracatuba Campus, Brazil, during the period between January 1991 and July 1998. The following variables were observed: age, color, profession, sex, smoking, alcohol intake, time of lesion evolution, macroscopy, oral hygiene, clinical state, treatment therapy and complications. Results: Age varied between 27 and 79 years, all of the patients were white, 54.8% did not work under the sun, 93.5% were of the male, 61.2% smoked, 51.6% drank, 54.8% were diagnosed after 6 months of the lesion appearing, 74.2% were ulcers, 48.4% had very poor oral hygiene, 87.% were at stage I and II, all were submitted to surgery, 9.7% presented post operative complications, 77.4% were degree II. Conclusion: It was concluded that lip carcinoma has a good prognosis, profession did not influence its occurrence, smokers had greater possibility of postoperative complications and the diagnosis is made several months after the lesion has appeared. Oral Cancer Center Paulista State University Aracatuba Campus Oral Cancer Center Paulista State University Aracatuba Campus
- Published
- 2001
10. Three different clinical manifestations of oral and systemic paracoccidioidomycosis
- Author
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Souza, R.C.N., primary, Vitorino, N.S., additional, Martins, J.R.D., additional, Provenzano, N., additional, and Furuse, C., additional
- Published
- 2011
- Full Text
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11. Langerhans cell histiocytosis in a child: a 10-year follow-up.
- Author
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De Camargo Moraes P, Bonecker M, Furuse C, Teixeira RG, and Araujo VC
- Published
- 2007
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12. Actinic Cheilitis among agricultural workers in Campinas, Brazil.
- Author
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Junqueira, J. L. R., Bönecker, M., Furuse, C., Morais, P. de Camargo, Flório, F. M., Cury, P. R., and Araújo, V. C.
- Abstract
Objective To assess the prevalence of Actinic Cheilitis (AC) among agricultural workers and analyze its risk factors. Design A cross sectional epidemiological study. A lip lesion was defined as an abnormal change on the lip mucosa surface, such as erythematous pigmented, ulcerative or swelling (Cataldo and Doku, 1981). Data were gathered according to age group, gender, ethnicity, time and frequency of occupational sunlight exposure, smoking habits, drinking habits and socio-economic status. Setting Sugar-cane plantation farms in Brazil. Participants Full-time workers of both genders employed at sugar-cane plantation farms for at least six months. Outcome measures Correlations between AC prevalence, demographic and socioeconomic risk factors. Results 202 people were examined and the prevalence of AC was 39.6%. Results revealed that being black (0.15-0.88- 95%CI; OR=0.36; p=0.025) or mulatto (0.21-0.82- 95%CI; OR=0.42; p=0.011 ) decreased the risk for AC, while age and gender sex had no effect. In relation to socioeconomic variables, formal education and more than four years of education (0.07-0.68- 95%CI; OR=0.22; p=0.009) decreased the risk for AC. Moreover, drinking alcohol was a risk for AC (1.05-3.37- 95%CI; OR=1.88; p=0.034), while tobacco smoking was not (0.60-2.02- 95%CI; OR=1.10; p= 0.763). Conclusions The prevalence of AC is high in agricultural workers who were fairskinned, had low education and high alcohol intake. Prevention and early diagnosis are required for workers exposed to sunlight. [ABSTRACT FROM AUTHOR]
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- 2011
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13. Tongue cancer in non-smoking and non-alcoholic mother and daughter.
- Author
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de Melo Cardoso D, Conrado Neto S, Urbano Collado F, Furuse C, Callestini R, Bonetti Valente V, Ricardo Biasoli É, Issamu Miyahara G, and Galera Bernabé D
- Subjects
- Female, Humans, Middle Aged, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell genetics, COVID-19 complications, Mothers, Non-Smokers statistics & numerical data, Aged, Tongue Neoplasms pathology
- Abstract
Tongue cancer is more prevalent in male smokers and alcoholics. Although an increased incidence of tongue cancer has been noted in non-smoking and non-alcoholic women, reports of its occurrence in mother and daughter are extremely rare. Here, we report a case of a non-smoking and non-alcoholic mother and her daughter diagnosed and treated surgically for tongue squamous cell carcinoma (SCC). The daughter is still being monitored and the mother died from complications from COVID-19 after 6 years of treatment. This report shows that OSCC should be considered in the differential diagnosis of oral ulcerated lesions in non-smoking and non-alcoholic women, especially if there is a family history of first-degree oral cancer., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
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- 2024
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14. HIV-related plasmablastic lymphoma causing extensive bone destruction in the mandible.
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Kayahara GM, Valente VB, Salzedas LMP, Passador-Santos F, Furuse C, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Adult, Female, Humans, Mandible pathology, Mouth pathology, HIV Infections complications, Lymphoma, Large B-Cell, Diffuse complications, Plasmablastic Lymphoma complications, Plasmablastic Lymphoma diagnosis, Plasmablastic Lymphoma pathology
- Abstract
Plasmablastic lymphoma (PBL) is a rare subtype of large B-cell lymphoma commonly associated with HIV infection. HIV-related PBL has a dismal prognosis. The aggressive clinical course of the disease may lead to the development of rapid-growing swellings, like several benign and malignant conditions. Herein, we reported the case of a 38-year-old woman with a painful swelling in the mandible initially diagnosed as an abscess derived from a tooth extraction. Intraoral examination revealed a painful swelling with reddish, white and purplish areas in the posterior region of the mandible without signs of infection. Imaging exams showed an extensive bone destruction in the left mandibular body. Histopathological examination revealed a high proliferation of plasmacytoid cells with nuclear hyperchromatism. Tumor cells were negative for CD20, and positive for Ki-67, CD138, IgG and lambda chain. The diagnosis of oral PBL was defined and serological test showed positivity for HIV. Eight months after starting treatment, the patient died due to complications of cancer treatment. Lymphoproliferative malignancies related to HIV infection should be included in the differential diagnosis of rapid-growing swellings in the oral cavity., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
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- 2022
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15. Social isolation stress facilitates chemically induced oral carcinogenesis.
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Verza FA, Valente VB, Oliveira LK, Kayahara GM, Crivelini MM, Furuse C, Biasoli ÉR, Miyahara GI, Oliveira SHP, and Bernabé DG
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- Animals, Cytokines metabolism, Inflammation Mediators metabolism, Male, Neoplasm Proteins metabolism, Rats, Rats, Wistar, 4-Nitroquinoline-1-oxide toxicity, Behavior, Animal, Depression metabolism, Depression pathology, Depression physiopathology, Head and Neck Neoplasms chemically induced, Head and Neck Neoplasms metabolism, Head and Neck Neoplasms pathology, Head and Neck Neoplasms physiopathology, Social Isolation, Squamous Cell Carcinoma of Head and Neck chemically induced, Squamous Cell Carcinoma of Head and Neck metabolism, Squamous Cell Carcinoma of Head and Neck pathology, Squamous Cell Carcinoma of Head and Neck physiopathology, Stress, Psychological metabolism, Stress, Psychological pathology, Stress, Psychological physiopathology
- Abstract
Social isolation has affected a large number of people and may lead to impairment of physical and mental health. Although stress resulting from social isolation may increase cancer progression, its interference on tumorigenesis is poorly known. In this study, we used a preclinical model to evaluate the effects of social isolation stress on chemically induced oral carcinogenesis. Sixty-two 21-day-old male Wistar rats were divided into isolated and grouped groups. After 90 days of age, the rats from both groups underwent oral carcinogenesis with 4-nitroquinoline 1-oxide (4NQO) for 20 weeks. All rats were assessed for depressive-like behavior and euthanized for oral squamous cell carcinoma (OSCC) diagnosis and measurement of inflammatory mediators in the tumor microenvironment. Social isolation stress increased the OSCC occurrence by 20.4% when compared to control. Isolated rats also showed higher tumor volume and cachexia than the grouped rats. Social isolation did not induce changes in the depressive-like behavior after carcinogenic induction. Tumors from stressed rats had increased levels of the inflammatory mediators, TNF-alpha, IL1-beta and MCP-1. The concentrations of TNF-alpha and MCP-1 were significantly increased in the large tumors from isolated animals. Higher tumor levels of TNF-alpha, IL-6, IL1-beta and MCP-1 were positively correlated with OSCC growth. This study provides the first evidence that social isolation stress may facilitate OSCC occurrence and tumor progression, an event accompanied by increased local levels of inflammatory mediators., Competing Interests: The authors have declared that no competing interests exist.
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- 2021
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16. Beta-adrenergic blocker inhibits oral carcinogenesis and reduces tumor invasion.
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Cecilio HP, Valente VB, Pereira KM, Kayahara GM, Furuse C, Biasoli ÉR, Miyahara GI, Oliveira SHP, and Bernabé DG
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- 4-Nitroquinoline-1-oxide administration & dosage, 4-Nitroquinoline-1-oxide toxicity, Animals, Carcinogenesis chemically induced, Carcinogens administration & dosage, Carcinogens toxicity, Cytokines immunology, Cytokines metabolism, Disease Progression, Humans, Male, Mouth Mucosa drug effects, Mouth Mucosa immunology, Mouth Mucosa pathology, Mouth Neoplasms chemically induced, Mouth Neoplasms pathology, Mouth Neoplasms prevention & control, Neoplasm Invasiveness prevention & control, Neoplasms, Experimental chemically induced, Neoplasms, Experimental pathology, Neoplasms, Experimental prevention & control, Rats, Rats, Wistar, Receptors, Adrenergic, beta metabolism, Signal Transduction drug effects, Squamous Cell Carcinoma of Head and Neck chemically induced, Squamous Cell Carcinoma of Head and Neck pathology, Squamous Cell Carcinoma of Head and Neck prevention & control, Tumor Microenvironment drug effects, Tumor Microenvironment immunology, Adrenergic beta-Antagonists administration & dosage, Carcinogenesis drug effects, Mouth Neoplasms drug therapy, Neoplasms, Experimental drug therapy, Propranolol administration & dosage, Squamous Cell Carcinoma of Head and Neck drug therapy
- Abstract
Purpose: Beta-adrenergic signaling can influence cancer progression and the use of beta blockers as adjuvant drugs in oncologic patients has been suggested. However, the involvement of beta-adrenergic blockers in tumorigenesis is poorly understood. This study investigated the action of beta-adrenergic blocker propranolol on tumor onset using a preclinical model of chemically induced oral cancer., Methods: Thirty-two male Wistar rats were subjected to daily subcutaneous injection of beta-blocker propranolol (10 mg/kg; SubQ), while another 32 rats received only a PBS injection (sham group). One week after starting propranolol treatment, all rats were submitted to chemical induction of oral carcinogenesis with 4-nitroquinoline-1-oxide (4NQO). After 16 weeks, they were assessed for occurrence of oral squamous cell carcinoma (OSCC), in addition to measurement of tumor volume and thickness, and tissue levels of cytokines IL-6, TNF-alpha and IL-10 in the tumor microenvironment., Results: Propranolol treatment reduced the occurrence of OSCC by 31%, 95% CI ( - 127, 216). Beta-adrenergic blocker significantly decreased thickness of OSCC when compared with PBS. Rats treated with propranolol exhibited a lower tumor volume when compared with control rats, but this result did not reach statistical significance. Tumors from propranolol-treated rats exhibited reduced concentrations of pro-inflammatory cytokines IL-6 and TNF-α. There was no difference in the IL-10 levels between tumors from propranolol- and sham-treated rats., Conclusion: Beta-adrenergic signaling may be one of the mechanisms associated with chemically induced oral carcinogenesis.
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- 2020
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17. Benign symmetric lipomatosis in the tongue: an uncommon case.
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Bastos DB, Figueira JA, Furuse C, Biasoli ÉR, Miyahara GI, and Bernabé DG
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- Aged, 80 and over, Biopsy, Diagnosis, Differential, Humans, Lipomatosis diagnosis, Lipomatosis, Multiple Symmetrical diagnosis, Male, Tongue pathology, Lipomatosis pathology, Tongue Neoplasms pathology
- Abstract
Symmetric lipomatosis of the tongue (SLT) is rare and characterized by diffuse growth and unencapsulated lipomas. An 87-year-old man was referred for evaluation of tongue lesions. Intraoral findings showed soft yellowish nodules with a smooth shiny surface diffusely on the lateral border of the tongue, bilaterally. Our clinical diagnosis was multiple tongue lipomas and an incisional biopsy was done. Histopathological examination revealed unencapsulated lobules of mature adipocytes with slight variation in the size and shape, confirming the diagnosis of lipoma. The final diagnosis was SLT. On follow-up at 6 months, the tongue findings were unchanged and no new lesions were observed.
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- 2020
18. Benign odontogenic ghost cell lesions revisited and new considerations on dysplastic dentin.
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Rosa ACG, Teixeira LN, Passador-Santos F, Furuse C, Montalli VÂM, de Araújo NS, and de Araújo VC
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- Collagen Type I, Humans, Keratins, Dentin, Odontogenic Cyst, Calcifying, Odontogenic Tumors
- Abstract
Objectives: This study aimed to revisit benign odontogenic ghost cell lesions (BOGCL) by hematoxylin and eosin staining and immunohistochemistry., Materials and Methods: Thirty cases of calcifying odontogenic cyst (COC) and 6 cases of dentinogenic ghost cell tumor (DGCT) were selected for histopathological and immunohistochemical analysis. Sections stained for cytokeratin (K) 14, K-19, amelogenin, collagen type 1 (COL-1), and dentin matrix acidic phosphoprotein 1 (DMP-1) were evaluated using qualitative analysis. Sections stained for Ki-67 and minichromosome maintenance protein-2 (MCM-2) were evaluated using semi-quantitative analysis., Results: A morphologic overlap was noticed in all BOGCL. Moreover, no differences were detected in the expression of K-14 and K-19. The expression of proliferative markers Ki-67 and MCM-2 was similar between cystic and tumor lesions (p > .05). The presence of COL-1 and absence of amelogenin in the so-called dysplastic dentin, associated with its histologic pattern, suggest that this is in fact an enameloid-like tissue., Conclusions: The dysplastic dentin should be considered an enameloid-like tissue in these lesions., Clinical Relevance: The similarity in histology, protein expression, and proliferative marker indices between COC and DGCT suggest that they are a sole entity and likely represent types of the same neoplasia.
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- 2019
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19. The Challenging Diagnosis of Primordial Odontogenic Tumor.
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Teixeira LN, Furuse C, Santos FP, Soares AB, de Oliveira EMF, de Araújo NS, and de Araújo VC
- Abstract
Primordial odontogenic tumor (POT) is a benign mixed odontogenic tumor comprised of a loose connective tissue with a similar morphology with dental papilla and exhibiting in its periphery the presence of a columnar epithelium. POT occurs in young patients and typically is associated with an unerupted tooth, with the mandible being the main anatomic site of occurrence. The present manuscript is aimed at describing a new case of POT and reviewing the main biologic findings related to this odontogenic tumor.
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- 2019
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20. Influence of weight gain on the modulation of wound healing following tooth extraction.
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Furuse C, Almeida AF, Costa SF, Ervolino-Silva AC, Okamoto R, Sumida DH, Matsumoto MA, and Leite FRM
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- Animals, Male, Obesity pathology, Random Allocation, Rats, Rats, Wistar, Tooth Extraction adverse effects, Tooth Socket pathology, Obesity metabolism, Osteogenesis physiology, Tooth Extraction trends, Tooth Socket metabolism, Weight Gain physiology, Wound Healing physiology
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- 2018
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21. A Combined Epithelial Odontogenic Tumor? A 7-Year Follow-Up Case.
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Rosa ACG, Soares AB, Furuse C, Lima SRR, de Araújo VC, and Passador-Santos F
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- Adolescent, Female, Follow-Up Studies, Humans, Mandibular Neoplasms pathology, Odontogenic Tumors pathology, Skin Neoplasms pathology
- Abstract
Adenomatoid odontogenic tumor (AOT) is a benign epithelial odontogenic tumor characterized by slow and progressive growth. When central lesions are associated with an unerupted permanent tooth, they are also known as the follicular type. Histological variants of AOT may complicate diagnosis with possible adverse effects on treatment and prognosis. The aim of this study is to report a case of a follicular AOT with extensive calcifying epithelial odontogenic tumor (CEOT)-like histopathological areas in the anterior region of the mandible that was treated by enucleation. The teeth displaced by the tumor were repositioned with orthodontic treatment, and after 7 years of follow-up, the bone trabeculae were normal with no evidence of recurrence. The clinical, radiographic and histopathologic aspects of these tumors are discussed and the debate surrounding whether these cases are true combined lesions or histologic variants of the primary tumor is raised.
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- 2017
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22. Factors that may influence polymorphous low-grade adenocarcinoma growth.
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Soares AB, Martinez EF, Ribeiro PF, Barreto IS, Aguiar MC, Furuse C, Sperandio M, Montalli VA, de Araújo NS, and de Araújo VC
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- Adult, Aged, Aged, 80 and over, Apoptosis physiology, Autophagy physiology, Biomarkers, Tumor analysis, Cell Proliferation physiology, Cellular Senescence physiology, Female, Humans, Immunohistochemistry, Male, Middle Aged, Adenocarcinoma pathology, Mouth Neoplasms pathology, Neovascularization, Pathologic pathology
- Abstract
There is mounting evidence on the importance of some biological processes in tumor growth, such as vascular supply, apoptosis, autophagy, and senescence. We have investigated these processes in polymorphous low-grade adenocarcinoma (PLGA), in an attempt to identify those that are relevant for this particular lesion. We analyzed 31 cases of PLGA using immunohistochemistry to antibodies against CD34 and CD105 to detect blood vessels; against D2-40 to detect lymphatic vessels; against Bax, Bcl-2, and survivin to explore cell apoptosis; and against Beclin and LCB3 to investigate autophagy and against p21 and p16 to assess senescence. Our results showed that PLGA growth does not depend on newly formed vessels but only on preexisting vasculature. Furthermore, PLGA is promoted by autophagy, sustained by both anti-apoptotic and anti-senescence signals, and stimulated by Bcl-2 and survivin.
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- 2017
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23. Mammaglobin and DOG-1 expression in polymorphous low-grade adenocarcinoma: an appraisal of its origin and morphology.
- Author
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Montalli VA, Passador-Santos F, Martinez EF, Furuse C, Aguiar MC, Soares FA, Soares AB, Brown AL, de Araújo NS, and de Araújo VC
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- Adenocarcinoma pathology, Adult, Aged, Aged, 80 and over, Female, Humans, Immunohistochemistry, Male, Middle Aged, Salivary Gland Neoplasms pathology, Adenocarcinoma metabolism, Anoctamin-1 metabolism, Biomarkers, Tumor metabolism, Mammaglobin A metabolism, Neoplasm Proteins metabolism, Salivary Gland Neoplasms metabolism
- Abstract
Background: Polymorphous low-grade adenocarcinoma (PLGA) remains a diagnostic challenge for most pathologists due to its large spectrum of histological patterns. In this study, the expression of two new markers recently described for salivary gland tumors was studied in PLGA., Methods: The morphology of 33 cases of PLGA was carefully evaluated using hematoxylin-and-eosin-stained sections and confirmed by immunohistochemistry for cytokeratin 7, vimentin, and S-100. Periodic acid-Schiff with diastase digestion was also used. The expression of mammaglobin and DOG-1 was carried out using the EnVision System. Mammaglobin was assessed according to the percentage of positively stained tumor cells, while DOG-1 was evaluated according to its presence and site. For MCM-2 and Ki-67, markers of proliferation, the labeling index of cell nuclei positivity was evaluated using total cell number. The ETV6-NTRK3 fusion was examined by fluorescence in situ hybridization analysis., Results: The histological patterns of the tumor were classified as lobular or non-lobular. For the non-lobular pattern, tubular, cribriform, glomeruliform, trabecular, and papillary patterns were observed. Mammaglobin was present in all PLGA cases, and its expression was stronger (P = 0.01) in the lobular than in the non-lobular pattern. The expression of DOG-1 was present in the apical portion and cytoplasm of the cells. Proliferation markers were low for all cases independent of histological pattern., Conclusions: Polymorphous low-grade adenocarcinoma has been confirmed to originate from the intercalated duct and to feature high expression of mammaglobin in its lobular pattern resembling that of mammary secretory analogue carcinoma, except for the ETV6 gene rearrangement., (© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2017
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24. Aggressive radiolucent lesion of the mandible.
- Author
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Valente VB, Barreto IS, Furuse C, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Aged, Biopsy, Fine-Needle, Combined Modality Therapy, Diagnosis, Differential, Diagnostic Imaging, Diphosphonates therapeutic use, Female, Humans, Immunohistochemistry, Risk Factors, Thyroidectomy, Mandibular Neoplasms secondary, Mandibular Neoplasms therapy, Thyroid Neoplasms pathology, Thyroid Neoplasms therapy
- Published
- 2016
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25. Immunoexpression of growth factors and receptors in polymorphous low-grade adenocarcinoma.
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Rosa AC, Soares AB, Santos FP, Furuse C, and de Araújo VC
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- Adenocarcinoma pathology, Cell Differentiation physiology, Cell Nucleus pathology, Cell Proliferation physiology, Cell Transformation, Neoplastic metabolism, Cell Transformation, Neoplastic pathology, Humans, Immunohistochemistry, Salivary Gland Neoplasms pathology, Salivary Glands, Minor pathology, Adenocarcinoma metabolism, Intercellular Signaling Peptides and Proteins biosynthesis, Receptors, Growth Factor biosynthesis, Salivary Gland Neoplasms metabolism, Salivary Glands, Minor metabolism
- Abstract
Background: Polymorphous low-grade adenocarcinoma (PLGA) is a rare malignant tumor that usually arises in the minor salivary glands. Growth factors are cell-secreted peptides that regulate biological processes such as growth, proliferation, and differentiation. In salivary gland tumors, immunoexpression of growth factors and their receptors is associated with cell proliferation, malignant transformation, and tumor invasion. This study analyzed the expression of growth factors and receptors in PLGA, in other to better understand the mechanisms involved in the process of neoplastic cell proliferation and tumor progression., Methods: The expression of growth factors FGF-2, PDGF-A, PDGF-B and receptors FGFR-1, FGFR-2, PDGFR-α, and EGFR was analyzed in 24 PLGA samples in comparison with normal salivary glands, by immunohistochemistry. A semi-quantitative analysis determined cell positivity in all stained sections. Scores were assigned according to percentage of reactive cells: score 0 < 10%; score 1-10 to 25%; score 2-25% to 50%; score 3->50%. The level of significance was set at 5%., Results: Most of the growth factors and receptors, apart from FGFR-2, were significantly reactive in PLGA. Comparing to salivary acini, all of the reactive growth factors and receptors were significantly stronger in PLGA. Comparing to salivary ducts, the expression of FGF-2, PDGF-B, FGFR-1, and EGFR was significantly stronger in the nuclei and/or cytoplasm of the neoplastic cells., Conclusions: The increased expression of the growth factors and receptors in the PLGA, compared to normal salivary glands, may be related to cell proliferation, somehow participating in the oncogenic process., (© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2016
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26. Prevalence of oral lesions in hospitalized patients with infectious diseases in northern Brazil.
- Author
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Gemaque K, Giacomelli Nascimento G, Cintra Junqueira JL, Cavalcanti de Araújo V, and Furuse C
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- Adolescent, Adult, Aged, Aged, 80 and over, Brazil epidemiology, Female, Hospitalization, Humans, Male, Middle Aged, Prevalence, Sex Factors, Young Adult, Communicable Diseases complications, Mouth Diseases complications, Mouth Diseases epidemiology
- Abstract
The aim of this study was to assess the prevalence of oral lesions in infectious-contagious diseases patients being treated in the University Hospital of the Federal University of Pará, northern Brazil. One hundred seven patients with infectious diseases were clinically investigated for oral lesions at the University Hospital of Pará, northern Brazil. From total sample, most patients were men (65.7%) with a mean age of 45.4 years. About prevalence of systemic diseases, tuberculosis was the most frequent illness, followed by AIDS, hepatitis types B and C, leishmaniasis, and meningitis. Analyzing oral manifestations, periodontal diseases and candidiasis were the most prevalent diseases in both genders, followed by recurrent aphthous ulcers, saburral tongue, simplex herpes, and squamous cell carcinoma. Of all 107 patients, only 10 males and 6 females did not present any oral manifestation. There was no statistical difference between genders with any systemic condition (P>0.05). The great prevalence of oral manifestations in hospitalized patients with systemic disorder emphasizes the need of integral dental care in this context, aiming at a multidisciplinary approach of patients. Therefore, presence of some oral conditions, such as candidiasis, should be an alert to different systemic conditions, once in assistance with physicians; dentists can influence the early diagnosis and treatment.
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- 2014
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27. In vitro evaluation of the suppressor potential of conditioned medium from benign myoepithelial cells from pleomorphic adenoma in malignant cell invasion.
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da Silva AD, Silva CA, Montalli VA, Martinez EF, de Araújo VC, and Furuse C
- Subjects
- Actins analysis, Biocompatible Materials chemistry, Breast Neoplasms pathology, Calcium-Binding Proteins analysis, Carcinoma in Situ pathology, Carcinoma, Ductal, Breast pathology, Carcinoma, Squamous Cell pathology, Cell Culture Techniques, Cell Line, Tumor, Coculture Techniques, Collagen chemistry, Diffusion Chambers, Culture, Drug Combinations, Epithelial Cells metabolism, Female, Humans, Laminin chemistry, Melanoma pathology, Membranes, Artificial, Microfilament Proteins analysis, Micropore Filters, Mouth Neoplasms pathology, Muscle Proteins analysis, Myocytes, Smooth Muscle metabolism, Proteoglycans chemistry, Tumor Microenvironment, Vimentin analysis, Calponins, Adenoma, Pleomorphic pathology, Culture Media, Conditioned, Neoplasm Invasiveness pathology
- Abstract
Tumoral invasion process is the result of a complex interaction between the tumor cells and microenvironment which plays an important role in modulating the growth and invasion of the cancer. The myoepithelial cells, present in glandular organs such as the breast and salivary glands, seem to exert paracrine effects on the glandular epithelium, acting as natural tumor suppressors. To verify the influence of the benign myoepithelial cells in the invasion of malignant cells, simulating an in situ carcinoma ex pleomorphic adenoma, we have cultured three different high-potential invasive malignant tumors (breast ductal adenocarcinoma, melanoma and oral squamous cell carcinoma) in conditioned medium of myoepithelial cells from salivary gland pleomorphic adenomas using transwell chambers with 8-μm pores membrane coated with matrigel. After 96 h, quantitative analyses of the results were performed by calculating the invasion index (number of cells that invaded in relation to the total number of cells). The results showed that there was a reduction of the invasion index mean for the three different malignant tumors. This study supports a tumoral suppressor function of the myoepithelial cells from pleomorphic adenoma in in vitro invasion process., (© 2012 John Wiley & Sons A/S.)
- Published
- 2012
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28. Oral arteriovenous hemangioma in patient with hepatitis C.
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Montalli VA, Martinez EF, Thomaz LA, Furuse C, and de Araújo VC
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- Female, Hemangioma metabolism, Hepatitis C complications, Hepatitis C metabolism, Hepatitis C therapy, Humans, Middle Aged, Mouth Neoplasms metabolism, Hemangioma pathology, Hepatitis C pathology, Mouth Neoplasms pathology
- Published
- 2012
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29. Expression of the vascular endothelial growth factor and angiopoietins in mucoepidermoid carcinoma of salivary gland.
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Demasi AP, Silva CA, Silva AD, Furuse C, Soares AB, Altemani A, Napimoga MH, and Araújo VC
- Subjects
- Adolescent, Adult, Aged, Carcinoma, Mucoepidermoid pathology, Child, Female, Fibroblasts metabolism, Fibroblasts pathology, Head and Neck Neoplasms metabolism, Head and Neck Neoplasms pathology, Humans, Immunohistochemistry, Male, Middle Aged, Parotid Neoplasms pathology, Receptor, TIE-2 metabolism, Tumor Microenvironment physiology, Angiopoietin-1 metabolism, Angiopoietin-2 metabolism, Carcinoma, Mucoepidermoid metabolism, Parotid Neoplasms metabolism, Vascular Endothelial Growth Factor A metabolism
- Abstract
Disrupted coordination of angiogenesis regulating signals, among them the vascular endothelial growth factor (VEGF) and angiopoietins (Angs), has been associated with abnormal angiogenesis and tumor progression. While VEGF induces endothelial cell proliferation, thereby initiating vessel formation, Angs are subsequently required for mural cell attachment, thus influencing remodeling and maturation of this vasculature. In addition to tumor cell, endothelial and mural cells, as well as myofibroblasts may also contribute to the secretion of these factors. In this study, we have analyzed by immunohistochemistry the expression of VEGF, Ang-1, Ang-2 and the Angs receptor Tie2 in both the stroma and tumor cells of mucoepidermoid carcinoma (MEC) of salivary gland. We have demonstrated that when myofibroblasts were detected adjacent to the cancer cells, they were frequently associated with intense positive staining for Ang-1 and Ang-2, and no reactivity to VEGF and Tie2. These myofibroblast-rich Ang-1 and Ang-2-stained areas were more commonly found in high-grade MEC cases than in low-grade ones. As for the malignant cells, they frequently expressed all proteins studied, but Ang-2 and VEGF were detected at higher levels compared to Ang-1 and Tie2. Our results indicate that the MEC environment favors cooperative activity between Angs and VEGF in modulating vascular growth and tumor aggressiveness.
- Published
- 2012
- Full Text
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30. Etiologic factors associated with oral squamous cell carcinoma in non-smokers and non-alcoholic drinkers: a brief approach.
- Author
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Vargas-Ferreira F, Nedel F, Etges A, Gomes AP, Furuse C, and Tarquinio SB
- Subjects
- Alcohol Drinking adverse effects, Carcinoma, Squamous Cell epidemiology, Humans, Mouth Neoplasms epidemiology, Papillomaviridae, Papillomavirus Infections epidemiology, Risk Factors, Smoking adverse effects, Carcinoma, Squamous Cell etiology, Mouth Neoplasms etiology, Papillomavirus Infections complications
- Abstract
Oral squamous cell carcinoma (OSCC) is the fifth most common type of cancer in the world. The effect of some etiological factors is well established in the literature, such as consumption of tobacco and alcohol. However, approximately 15 to 20% of all oral cancer cases occur in patients without the traditional risk factors, reflecting in numerous cases of OSCC in non-smokers and non-alcoholic drinkers. Also, several studies have suggested a possible association between human papillomavirus and OSCC. Under these aspects, the purpose of this study is to address cases of oral cancer in non-smokers and non-drinkers focusing on the role of HPV, thus contributing to improve the diagnosis and monitoring of OSCC. A computer database search was performed using the Pubmed database. The search key words were: epidemiology, oral squamous cell carcinoma, risk factors and human papillomavirus. It is important to assess patients without the potential risk factors, since this knowledge may help identifying other features associated with the occurrence of oral cancer, enabling an appropriate clinical management and monitoring.
- Published
- 2012
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31. Recurrent oral pyogenic granuloma in port-wine stain.
- Author
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da Silva AD, Silva CA, de Camargo Moraes P, Thomaz LA, Furuse C, and de Araújo VC
- Subjects
- Adult, Combined Modality Therapy, Female, Granuloma, Pyogenic complications, Humans, Middle Aged, Mouth Diseases complications, Port-Wine Stain complications, Recurrence, Granuloma, Pyogenic therapy, Mouth Diseases therapy, Port-Wine Stain therapy
- Abstract
Pyogenic granuloma (PG) is a benign inflammatory lesion, nonneoplastic in nature, which occurs in the oral cavity and skin. This lesion arises in response to various stimuli such as low-grade local irritations, traumatic injury, or hormonal factors. Recently, in some cases, the occurrence of recurrent PGs in skin associated with vascular lesions, such as port-wine stains, has been described. It has been postulated that this association is promoted by arteriovenous anastomoses in the vascular lesions, leading to the development of PG. The authors discuss 2 cases of recurrent PG in patients with a port-wine stain, and the treatment options adopted.
- Published
- 2011
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32. Trabecular juvenile ossifying fibroma with aneurysmal bone cyst: a rare presentation.
- Author
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Silva CA, Silva AD, Soares JA, Furuse C, de Araújo NS, and de Araújo VC
- Subjects
- Bone Cysts, Aneurysmal pathology, Child, Facial Asymmetry etiology, Female, Fibroma, Ossifying pathology, Humans, Maxillary Diseases pathology, Maxillary Neoplasms pathology, Neoplasm Recurrence, Local, Bone Cysts, Aneurysmal complications, Fibroma, Ossifying complications, Maxillary Diseases complications, Maxillary Neoplasms complications
- Abstract
Juvenile ossifying fibroma is an uncommon, benign, bone-forming neoplasm that is distinguished from other fibro-osseous lesions primarily by its age of onset, clinical presentation, and potential behavior. It mainly occurs in juveniles and has a slight male predilection and more aggressive behavior than a common ossifying fibroma. There are 2 distinct histopathological variants of this lesion: (1) psammomatoid pattern and (2) trabecular pattern. An aneurysmal bone cyst may occur in association with other bone lesions, such as fibrous dysplasia, ossifying fibroma, and giant cell lesion. The clinical management and prognosis of juvenile ossifying fibroma is somewhat uncertain, and this tumor has high rates of recurrence. Such behavior may be related to younger patient age and the concurrent development of aneurysmal bone cyst. The purpose of this paper was to report a case of trabecular juvenile ossifying fibroma in a 9-year-old girl associated with an aneurysmal bone cyst, presenting an aggressive behavior, and causing significant facial asymmetry.
- Published
- 2011
33. Immunohistochemical study of stromal and vascular components of tonsillar polyps: high endothelial venules as participants of the polyp's lymphoid tissue.
- Author
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Barreto I, Costa AF, Martins MT, Furuse C, de Araújo VC, and Altemani A
- Subjects
- Adolescent, Adult, Biomarkers metabolism, Child, Endothelium, Lymphatic metabolism, Female, Humans, Male, Middle Aged, Palatine Tonsil metabolism, Palatine Tonsil surgery, Polyps metabolism, Polyps surgery, Stromal Cells metabolism, Tonsillectomy, Venules metabolism, Venules pathology, Young Adult, Endothelium, Lymphatic pathology, Palatine Tonsil pathology, Polyps pathology, Stromal Cells pathology
- Abstract
Tonsillar polyps are nonneoplastic lesions usually composed of variable amounts of lymphoid and vascular and connective tissues. All of them are generally assumed to be hamartomatous proliferations, but the profile of vascular and connective components has yet to be explored. The vascular system of the tonsils is complex and includes highly specialized structures (i.e., high endothelial venules (HEVs)) involved in lymphocyte homing into lymphoid tissues. In 14 tonsillar polyps and 26 control tonsils, an immunohistochemical study was performed using CD34 (blood vessels and HEVs), MECA-79 (HEVs), D2-40 (lymphatic vessels), Ki-67, collagens I and III, fibronectin, and tenascin-C. The polyps showed increased total lymphatic area, whereas the number of blood vessels and lymphatics and the blood vascular area did not differ significantly from those of control tonsils. Rare Ki-67+ endothelial cells were found. In the polyps, we detected, possibly for the first time, HEVs amid lymphoid tissue, and that the amount of the latter correlated positively with HEV density. The polyps also presented lesser amounts of fibronectin and collagens I and III than in normal tonsils, which were distributed in a disorganized fashion. Tenascin-C expression was uncommon in the polyps and control tonsils. Tonsillar polyps are composed of disorganized connective tissue and lymphatic channels which can be considered hamartomatous proliferations. However, the lymphoid component is possibly reactive due to its relationship with the HEVs. The highly differentiated phenotype of the HEVs and their complex biology are not in agreement with what would be expected for a component of hamartomatous nature.
- Published
- 2011
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34. Study of growth factors and receptors in carcinoma ex pleomorphic adenoma.
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Furuse C, Miguita L, Rosa AC, Soares AB, Martinez EF, Altemani A, and de Araújo VC
- Subjects
- Adult, Aged, Carcinoma in Situ pathology, Cell Proliferation, Coloring Agents, Disease Progression, Epithelial Cells pathology, ErbB Receptors analysis, Female, Fibroblast Growth Factors analysis, Hepatocyte Growth Factor analysis, Humans, Male, Middle Aged, Neoplasm Invasiveness, Protein Serine-Threonine Kinases analysis, Proto-Oncogene Proteins c-met analysis, Receptor, Fibroblast Growth Factor, Type 1 analysis, Receptor, Fibroblast Growth Factor, Type 2 analysis, Receptor, IGF Type 1 analysis, Receptor, Transforming Growth Factor-beta Type II, Receptors, Transforming Growth Factor beta analysis, Submandibular Gland Neoplasms pathology, Transforming Growth Factor beta1 analysis, Adenocarcinoma pathology, Adenoma, Pleomorphic pathology, Intercellular Signaling Peptides and Proteins analysis, Parotid Neoplasms pathology, Receptors, Growth Factor analysis
- Abstract
Carcinoma ex pleomorphic adenoma (CXPA) is a rare malignant salivary gland tumor derived from a pre-existing pleomorphic adenoma. It is a good model to study the evolution of carcinogenesis, starting with in situ areas to frankly invasive carcinoma. Growth factors are associated with several biological and neoplastic processes by transmembrane receptors. In order to investigate, by immunohistochemistry, the expression of some growth factors and its receptors [EGF receptor, fibroblast growth factor, fibroblast growth factor receptor 1, fibroblast growth factor receptor 2, hepatocyte growth factor, c-Met, transforming growth factor (TGF) beta1, TGFbetaR-II and insulin-like growth factor receptor 1] in the progression of CXPA, we have used ten cases of CXPA in several degrees of invasion- intracapsular, minimally and frankly invasive carcinoma- with only epithelial component. Slides were qualitatively and semi-quantitatively evaluated according to the percentage of stained tumor cells from 0 to 3 (0 = less than 10%; 1 = 10-25%; 2 = 25-50%; 3 = more than 50% of cells). Malignant epithelial cells starting with in situ areas showed stronger expression than luminal cells of pleomorphic adenoma for all antibodies. Most of the intracapsular, minimally and frankly invasive CXPA presented score 3. However, score 2 was more evident in the frankly invasive one. In small nests of invasive carcinoma, negative cells were observed probably indicating that the proliferative process is replaced by the invasive mechanism. Altogether this data infers that these factors may contribute to cell proliferation during initial phases of the tumor.
- Published
- 2010
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35. Necrotizing sialometaplasia in a patient who is HIV positive: a case report.
- Author
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Silva AD, Silva CA, Furuse C, Nunes e Souza RC, da Costa MH, and de Araújo VC
- Subjects
- Carcinoma, Squamous Cell diagnosis, Diagnosis, Differential, Humans, Male, Middle Aged, Mouth Neoplasms diagnosis, Oral Ulcer diagnosis, HIV Seropositivity complications, Palate, Hard pathology, Salivary Glands, Minor pathology, Sialometaplasia, Necrotizing diagnosis
- Abstract
Necrotizing sialometaplasia (NS) is a self-limiting, benign, inflammatory disease of the minor salivary glands of the hard palate. The main significance of the NS lesion lies in the fact that it may be mistaken for mucoepidermoid or squamous cell carcinoma. A case report is presented of a patient with NS who was HIV positive; the lesion was located in the minor salivary glands of the hard palate. Clinically, the lesion presented as a deep ulcer with slightly elevated irregular borders and a necrotic base in the hard palate. Histologically, the tissue was characterized by squamous metaplasia of ducts and acini, lobular coagulation necrosis, and pseudoepitheliomatous hyperplasia of the overlying epithelium. The lesion disappeared completely after 2 weeks.
- Published
- 2010
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36. Glucose transporter protein 1 expression in mucoepidermoid carcinoma of salivary gland: correlation with grade of malignancy.
- Author
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Demasi AP, Costa AF, Altemani A, Furuse C, Araújo NS, and Araújo VC
- Subjects
- Adolescent, Adult, Aged, Autoantigens metabolism, Biomarkers, Tumor metabolism, Carcinoma, Mucoepidermoid immunology, Carcinoma, Mucoepidermoid pathology, Female, Humans, Immunohistochemistry, Male, Middle Aged, Mitochondria immunology, Peroxiredoxins metabolism, Salivary Gland Neoplasms immunology, Salivary Gland Neoplasms pathology, Young Adult, Carcinoma, Mucoepidermoid metabolism, Glucose Transporter Type 1 metabolism, Salivary Gland Neoplasms metabolism
- Abstract
Mucoepidermoid carcinoma (MEC), the most common primary salivary malignancy, shows great variability in clinical behaviour, thus demanding investigation to identify of prognostic markers. Since Warburg's studies, unrestricted cell growth during tumorigenesis has been linked to altered metabolism, implying hypoxic stimulation of glycolysis and diminished contribution of mitochondrial oxidative phosphorylation to cellular ATP supply. Hypothesizing that the study of MEC metabolic status could lead to the discovery of prognostic markers, we investigated by immunohistochemistry the expression of glucose transporter 1 (Glut-1), mitochondrial antigen and peroxiredoxin I (Prx I) in samples of MEC from different histological grades. Our results showed that mitochondrial antigen and Prx I were expressed in the majority of the MEC cases independent of the histological grade. In contrast Glut-1 expression increased significantly as the tumours became more aggressive. These results suggested that oxidative phosphorylation may contribute to ATP supply in all stages of MEC progression, and that the relative contribution of glycolysis over mitochondria for cellular ATP supply increases during MEC progression, favouring growth under low oxygen concentration. In addition, the observed high Prx I protein levels could provide protection to tumour cells against reactive oxygen species generated as a consequence of mitochondrial function and hypoxia-reoxygenation cycling. Altogether our findings suggest that upregulation of Glut-1 and Prx I constitute successful adaptive strategies of MEC cells conferring a growth advantage over normal salivary gland cells in the unstable oxygenation tumour environment.
- Published
- 2010
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37. CD10 (Neutral Endopeptidase) Expression in Myoepithelial Cells of Salivary Neoplasms.
- Author
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Neves Cde O, Soares AB, Costa AF, de Araujo VC, Furuse C, Juliano PB, and Altemani A
- Subjects
- Actins metabolism, Aged, Biomarkers, Tumor metabolism, Cell Differentiation, Cell Transformation, Neoplastic, Diagnosis, Differential, Disease Progression, Epithelial Cells pathology, Female, Humans, Immunohistochemistry, Male, Middle Aged, Myocytes, Smooth Muscle pathology, Myoepithelioma enzymology, Myoepithelioma pathology, Salivary Gland Neoplasms enzymology, Salivary Gland Neoplasms pathology, Epithelial Cells metabolism, Myocytes, Smooth Muscle metabolism, Myoepithelioma diagnosis, Neprilysin metabolism, Salivary Gland Neoplasms diagnosis
- Abstract
CD10 is a cell surface peptidase expressed in a wide variety of normal and neoplastic tissues, including breast myoepithelial cells. In salivary glands, expression of CD10 has only been used to identify neoplastic myoepithelial cells of pleomorphic adenomas and myoepithelial carcinomas. However, its accuracy in other salivary tumors with myoepithelial component has yet to be analyzed. We examined 72 salivary tumors with myoepithelial differentiation using immunohistochemical technique to detect CD10. In salivary glands, CD10 expression was not detected in myoepithelial cells. Only fibrocytes within the intralobular stroma were CD10 positive. In neoplastic myoepithelial cells, CD10 expression was found in 25.71% of benign and 32.43% of malignant neoplasms. When the different groups of tumors were compared, epithelial-myoepithelial carcinomas (EMEC) showed a stark contrast with the others (83.3% of cases with CD10 expression). Surprisingly, adenoid cystic carcinomas and basal cell adenomas were negative in 100% of the cases. Myoepitheliomas, pleomorphic adenomas, and myoepithelial carcinomas were positive in 27.7%, 30.0%, and 40% of the cases, respectively. In conclusion, salivary neoplastic myoepithelial cells gain CD10 expression in relation to their normal counterparts. However, the gain of this protein is not a sensitive marker for detecting myoepithelial cells in the majority of the tumors, except for EMEC. The high expression of CD10 by this carcinoma can be a valuable tool to separate EMEC from the tubular variant of adenoid cystic carcinomas in small incisional biopsies, where the precise diagnosis may be impossible.
- Published
- 2010
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38. Mucocele of the gland of Blandin-Nuhn: histological and clinical findings.
- Author
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de Camargo Moraes P, Bönecker M, Furuse C, Thomaz LA, Teixeira RG, and de Araújo VC
- Subjects
- Adolescent, Adult, Child, Female, Humans, Male, Middle Aged, Young Adult, Mucocele pathology, Salivary Gland Diseases pathology, Salivary Glands, Minor pathology, Tongue Diseases pathology
- Abstract
The authors examined the clinical and histopathological features of 48 cases of mucocele of the glands of Blandin-Nuhn. Data of all patients with mucocele treated at the Department of Oral and Maxillofacial Surgery totaled 312 cases during the 6-year period of study were analyzed. There was no sex predominance, and most patients were younger than 20 years. All lesions were located on the ventral surface of the tongue, and they were more frequently observed at the tip of the tongue whereas few ones occurred more posteriorly. All lesions presented a polypoid appearance. Histopathologically, the mucoceles were of the extravasation type and trauma might be the most frequent etiological factor. This type of mucocele was the second most frequent one and should not be considered rare.
- Published
- 2009
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39. Collagen type I may influence the expression of E-cadherin and beta-catenin in carcinoma ex-pleomorphic adenoma.
- Author
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Araújo VC, Demasi AP, Furuse C, Altemani A, Alves VA, Freitas LL, and Araújo NS
- Subjects
- Adenoma, Pleomorphic pathology, Adherens Junctions metabolism, Adherens Junctions pathology, Adult, Aged, Cell Adhesion, Female, Humans, Male, Middle Aged, Neoplasm Invasiveness, Salivary Gland Neoplasms pathology, Adenoma, Pleomorphic metabolism, Cadherins biosynthesis, Collagen Type I metabolism, Gene Expression Regulation, Neoplastic, Neoplasm Proteins biosynthesis, Salivary Gland Neoplasms metabolism, beta Catenin biosynthesis
- Abstract
Carcinoma ex-pleomorphic adenoma (CXPA) is an aggressive salivary gland malignancy, usually derived from a long-standing or a recurrent benign tumor, the pleomorphic adenoma (PA). In the context of dynamic reciprocity, changes in the composition and structure of extracellular matrix proteins and cell surface receptors have been frequently associated with dysfunctional adhesion and invasive behavior of tumor cells. It is not fully understood if these changes are involved in the conversion of PA to CXPA. In this study, different progression stages of CXPA were investigated regarding the expression of the major extracellular matrix proteins, collagen type I, and of E-cadherin and beta-catenin, the components of adherens junctions. By immunohistochemical analysis, we have demonstrated that direct contact of tumor cells with fibrillar type I collagen, particularly near the invasive front and in invasive areas prevailing small nests of CXPA cells, could be associated with reduced expression of the E-cadherin and beta-catenin adhesion molecules and with invasive behavior of epithelial, but not of CXPA with myoepithelial component. Our results also suggested that this association could depend on the organization of collagen molecules, being prevented by high-order polymeric structures. These findings could implicate the local microenvironment in the transition from the premalignant PA to invasive CXPA.
- Published
- 2009
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40. Gardner syndrome with no clinical family history.
- Author
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Silva CA, Moraes Pde C, Furuse C, Junqueira JL, Thomaz LA, and de Araújo VC
- Subjects
- Adult, Biopsy, Bone Neoplasms diagnosis, Colonoscopy, Diagnosis, Differential, Female, Head and Neck Neoplasms diagnosis, Humans, Osteoma diagnosis, Sigmoidoscopy, Tomography, X-Ray Computed, Gardner Syndrome diagnosis
- Abstract
Gardner syndrome, a variant of familial adenomatous polyposis, is a hereditary disorder inherited as an autosomal dominant with high penetrance and variable expression that is caused by a mutation of the adenomatous polyposis coli gene. It is characterized by gastrointestinal polyps associated with multiple osteomas, dental anomalies, skin and soft tissue tumors, and congenital hypertrophy of the retinal pigment epithelium. Early diagnosis of Gardner syndrome is crucial and may be life saving because the polyps usually undergo malignant change by the fourth decade of life. The extraintestinal manifestations frequently precede gastrointestinal symptoms; consequently, the dentist may play an important role in the diagnosis of this syndrome. The authors report a case of Gardner syndrome with no clinical family history that was diagnosed by the dentist based on oral and maxillofacial findings.
- Published
- 2009
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41. Peroxiredoxin I is overexpressed in oncocytic lesions of salivary glands.
- Author
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Demasi AP, Furuse C, Altemani A, Junqueira JL, Oliveira PR, and Araújo VC
- Subjects
- Adenocarcinoma enzymology, Adenocarcinoma pathology, Adenolymphoma enzymology, Adenolymphoma pathology, Adenoma, Oxyphilic enzymology, Adenoma, Oxyphilic pathology, Antioxidants analysis, Biomarkers analysis, Carcinoma, Mucoepidermoid enzymology, Carcinoma, Mucoepidermoid pathology, Free Radical Scavengers analysis, Gene Expression Regulation, Enzymologic, Granular Cell Tumor enzymology, Granular Cell Tumor pathology, Humans, Hydrogen Peroxide analysis, Hyperplasia, Lysosomes pathology, Metaplasia, Mitochondria pathology, Oxyphil Cells pathology, Reactive Oxygen Species analysis, Salivary Gland Neoplasms enzymology, Salivary Gland Neoplasms pathology, Salivary Glands pathology, Thyroid Gland pathology, Oxyphil Cells enzymology, Peroxiredoxins analysis, Salivary Glands enzymology
- Abstract
Background: Oncocytic lesions, particularly frequent in the salivary glands, are characterized by cells with an atypical accumulation of mitochondria. This accumulation has been recognized as a compensatory mechanism to intrinsic functional defects of these organelles, resulting in energy production impairment and increased generation of reactive oxygen species (ROS), including hydrogen peroxide (H(2)O(2)). Peroxiredoxin I (Prx I) is a H(2)O(2) scavenging protein and the expression of its yeast homolog was reported to be influenced by mitochondrial function., Methods: In this study, we evaluated Prx I expression in oncocytic lesions of salivary glands by immunohistochemistry., Results: Our results showed that Prx I is overexpressed in oncocytes regardless of the salivary gland lesion where they appear., Conclusions: These results suggest that Prx I expression in oncocytes is related to its ability to decompose mitochondrial-derived H(2)O(2) and that it could provide to the cells a protective role in an environment that, by continuously producing potential DNA-damaging ROS, predisposes to genome instability and cellular transformation.
- Published
- 2009
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42. Peroxiredoxin I, platelet-derived growth factor A, and platelet-derived growth factor receptor alpha are overexpressed in carcinoma ex pleomorphic adenoma: association with malignant transformation.
- Author
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Demasi AP, Furuse C, Soares AB, Altemani A, and Araújo VC
- Subjects
- Adenocarcinoma pathology, Adenoma, Pleomorphic pathology, Adult, Aged, Biomarkers, Tumor metabolism, Cell Transformation, Neoplastic pathology, Humans, Male, Middle Aged, Neoplasms, Second Primary pathology, Salivary Gland Neoplasms pathology, Salivary Glands metabolism, Salivary Glands pathology, Signal Transduction, Adenocarcinoma metabolism, Adenoma, Pleomorphic metabolism, Cell Transformation, Neoplastic metabolism, Neoplasms, Second Primary metabolism, Peroxiredoxins metabolism, Receptor, Platelet-Derived Growth Factor alpha metabolism, Salivary Gland Neoplasms metabolism
- Abstract
Carcinoma ex pleomorphic adenoma is a rare salivary gland malignancy. It constitutes an important model for the study of carcinogenesis, as it can display the tumor in different stages of progression, from benign pleomorphic adenoma to frankly invasive carcinoma. Growth signaling pathways undergo continuous activation in human tumors, commonly as a consequence of the overexpression of ligands and receptors such as platelet-derived growth factor and platelet-derived growth factor receptor. Hydrogen peroxide is produced after platelet-derived growth factor receptor activation, and it is essential for the sequential phosphorylation cascade that drives cell proliferation and migration. By their ability to degrade hydrogen peroxide, peroxiredoxins are involved in growth factor signaling regulation and in the oxidative stress response. To verify the potential association of peroxiredoxin I, platelet-derived growth factor-A, and platelet-derived growth factor receptor-alpha with carcinoma ex pleomorphic adenoma progression, we investigated the expression of these molecules in carcinoma ex pleomorphic adenoma showing different degrees of invasion. The peroxiredoxin I, platelet-derived growth factor-A, and platelet-derived growth factor receptor-alpha proteins were present in remnant pleomorphic adenoma to only a small extent, but, collectively, they were highly expressed as soon as the malignant phenotype was achieved and remained at elevated concentrations during progression to the advanced stages of carcinoma ex pleomorphic adenoma. In addition, their locations overlapped significantly, strengthening their connection to this growth-signaling pathway. Our results indicate that carcinoma ex pleomorphic adenoma cells acquire at least 2 significant advantages relative to their normal counterparts: resistance to oxidative stress-induced apoptosis, conferred by high peroxiredoxin I concentrations, and sustained growth, reflecting platelet-derived growth factor-A and platelet-derived growth factor receptor-alpha overexpression.
- Published
- 2009
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43. Dentin matrix protein 1 (DMP1) expression in developing human teeth.
- Author
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Martinez EF, da Silva LA, Furuse C, de Araújo NS, and de Araújo VC
- Subjects
- Extracellular Matrix metabolism, Extracellular Matrix Proteins genetics, Fetal Development, Gene Expression, Humans, Immunohistochemistry, Odontoblasts metabolism, Odontogenesis physiology, Phosphoproteins genetics, Extracellular Matrix Proteins biosynthesis, Phosphoproteins biosynthesis, Tooth Germ metabolism
- Abstract
Dentin matrix protein 1 (DMP1) is an acidic phosphoprotein that plays an important role in mineralized tissue formation by initiation of nucleation and modulation of mineral phase morphology. The purpose of the present study was to examine the immunoexpression of DMP1 in tooth germs of 7 human fetuses at different gestational ages (14, 16, 19, 20, 21, 23 and 24 weeks) comparing with completed tooth formation erupted teeth. The results showed the presence of DMP1 in the dental lamina, as well as in the cells of the external epithelium, stellate reticulum and stratum intermedium of the enamel organ. However, in the internal dental epithelium, cervical loop region and dental papilla some cells have not labeled for DMP1. In the crown stage, DMP1 was expressed in the ameloblast and odontoblast layer, as well as in the dentinal tubules of coronal dentin near the odontoblast area. Erupted teeth with complete tooth formation exhibited immunolabeling for DMP1 only in the dentinal tubules mainly close to the dental pulp. No staining was observed in the enamel, predentin or dental pulp matrix. DMP1 is present in all developing dental structures (dental lamina, enamel organ, dental papilla) presenting few immunoexpression variations, with no staining in mineralized enamel and dentin.
- Published
- 2009
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44. Intravascular papillary endothelial hyperplasia: report of 2 cases and immunohistochemical study.
- Author
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Soares AB, Altemani A, Furuse C, Demasi AP, Gati C, Nunes N, and de Araújo VC
- Subjects
- Actins analysis, Adult, Aged, Antigens, CD34 analysis, Diagnosis, Differential, Female, Fibrillar Collagens analysis, Hemangiosarcoma diagnosis, Humans, Hyperplasia pathology, Immunohistochemistry, Laminin analysis, Lip Diseases pathology, Mouth Mucosa pathology, Mouth Neoplasms diagnosis, Vimentin analysis, Endothelium, Vascular pathology, Mouth Diseases pathology, Vascular Diseases pathology
- Abstract
Intravascular papillary endothelial hyperplasia (IPEH) is a benign, nonneoplastic, vascular lesion. The main significance of the lesion lies in the fact that it may be mistaken for angiosarcoma. Oral lesions are uncommon and the present paper reports 2 cases of oral IPEH, in different sites. Histologically, the tissue was characterized by papillary fronds lined by proliferating endothelium. Immunohistochemically (IHC), the lesion was positive for CD34, smooth muscle actin (SMA), type I and IV collagen, vimentin, and laminin, but it was negative for CD105. Local excision was the treatment of choice. No recurrence was observed during a 1-year and 6-month follow-up period, respectively. The clinical, histological, and immunohistochemical characteristics are discussed.
- Published
- 2008
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45. Interstitial and Langerhans' dendritic cells in chronic periodontitis and gingivitis.
- Author
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Cury PR, Furuse C, Rodrigues AE, Barbuto JA, Araújo VC, and Araújo NS
- Subjects
- Adult, Antigens, CD1 analysis, Antigens, CD1 immunology, Biomarkers analysis, Biopsy, Factor XIIIa analysis, Factor XIIIa immunology, Female, Gingivitis immunology, Humans, Langerhans Cells immunology, Male, Monocytes, Statistics, Nonparametric, Chronic Periodontitis pathology, Gingiva pathology, Gingivitis pathology, Langerhans Cells pathology
- Abstract
The aim of the present study was to compare quantitatively the distribution of dendritic cell subpopulations in chronic periodontitis and gingivitis. Fourteen biopsies from patients with chronic periodontitis and fifteen from patients with gingivitis were studied. An immunoperoxidase technique was used to quantify the number of Langerhans' cells (CD1a) and interstitial dendritic cells (factor XIIIa) in the oral and sulcular and junctional/pocket epithelia and in the lamina propria. A greater number of factor XIIIa+ dendritic cells in the lamina propria and CD1a+ dendritic cells in the oral epithelium were observed in gingivitis compared to the periodontitis group (p = 0.05). In the sulcular and junctional/pocket epithelia and in the lamina propria, the number of CD1a+ dendritic cells was similar in the gingivitis and periodontitis groups. In conclusion, the number of Langerhans' cells in the oral epithelium and interstitial dendritic cells in the lamina propria is increased in gingivitis compared to periodontitis, which may contribute to the different pattern of host response in these diseases.
- Published
- 2008
- Full Text
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46. STAT3 expression in salivary gland tumours.
- Author
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de Araújo VC, Furuse C, Cury PR, Altemani A, and de Araújo NS
- Subjects
- Adenocarcinoma metabolism, Adenoma, Pleomorphic metabolism, Carcinoma, Adenoid Cystic metabolism, Carcinoma, Mucoepidermoid metabolism, Female, Humans, Immunohistochemistry, Male, Biomarkers, Tumor metabolism, STAT3 Transcription Factor metabolism, Salivary Gland Neoplasms metabolism
- Abstract
The aim of the present study was to evaluate the signal transducer and activator of transcription (STAT3) expression, which is constitutively active in different types of malignant tumours, in salivary gland tumours. Fifty biopsies of salivary gland tumours (9 pleomorphic adenomas, 12 adenoid cystic carcinomas, 7 epithelial-myoepithelial carcinomas, 10 polymorphous low-grade adenocarcinomas and 12 mucoepidermoid carcinomas) and 10 normal salivary glands were immunohistochemically labeled for STAT3 and Phospho-STAT3 (STAT3P). The labeled sections were qualitatively and quantitatively evaluated. The results showed that, in normal salivary gland, STAT3 was expressed in cytoplasm and STAT3P in nuclei of all tissue cells, except in large mucous acinar cells for which both antibodies were negative. In pleomorphic adenoma, the expression was the same as in normal glands. In malignant tumours, there were variations in the expression of these antibodies. The most important one was the presence of STAT3 in the nuclei of the malignant tumour cells, most evident in the cribriform-type of adenoid cystic carcinoma. Both loss and variation of STAT3P expression were also observed. The presence of STAT3 in the nuclei of malignant salivary gland tumours may represent an important event in oncogenesis probably contributing to tumour cell proliferation while blocking apoptosis. However, further investigation will be necessary to support this hypothesis.
- Published
- 2008
- Full Text
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47. Effect of meloxicam and diclofenac sodium on peri-implant bone healing in rats.
- Author
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Pablos AB, Ramalho SA, König B Jr, Furuse C, de Araújo VC, and Cury PR
- Subjects
- Animals, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Fluorescent Dyes, Implants, Experimental, Male, Meloxicam, Rats, Rats, Sprague-Dawley, Statistics, Nonparametric, Tibia surgery, Wound Healing drug effects, Anti-Inflammatory Agents, Non-Steroidal adverse effects, Dental Implants, Diclofenac adverse effects, Osseointegration drug effects, Osteogenesis drug effects, Thiazines pharmacology, Thiazoles pharmacology
- Abstract
Background: This study evaluated the effects of diclofenac sodium and meloxicam on peri-implant bone healing., Methods: Thirty male rats were divided into three groups: the control group (CG) received no drug; the diclofenac sodium group (DSG) received 1.07 mg/kg twice a day for 5 days; and the meloxicam group (MG) received 0.2 mg/kg daily for 5 days. A screw-shaped titanium implant was placed in the tibia. Fluorochromes, oxytetracycline (OxT), calcein (CA), and alizarin (AL), were injected at 7, 14, and 21 days, respectively, after implantation, and the animals were sacrificed 28 days after implant placement. The percentages of OxT-, CA-, and AL-labeled bone as well as the percentages of bone-to-implant contact (BIC), cortical bone area (CBA), and trabecular bone area (TBA) within the implant threads were evaluated., Results: Bone healing was delayed in the DSG during the first 14 days after implant placement (OxT-labeled bone: DSG: 5.3% +/- 7.3% versus CG: 13.2% +/- 9.8%, P = 0.002, and versus MG:14.4% +/- 13.1%, P = 0.05). The percentages of BIC (DSG: 49.6% +/- 21.9%; MG: 67.1% +/- 22.8%; and CG: 68.1% +/- 22.8%) and CBA (DSG: 63.7% +/- 21.2%; MG: 82.7% +/- 12.4%; CG: 84.9% +/- 10.6%) were lower in the DSG compared to the MG and CG (P <0.001). The percentage of TBA was significantly greater in the DSG compared to the MG and CG (DSG: 36.3% +/- 21.2% versus MG: 17.3% +/- 12.7% and versus CG: 15.1% +/- 10.6%; P <0.001)., Conclusion: Diclofenac sodium seemed to delay peri-implant bone healing and to decrease BIC, whereas meloxicam had no negative effect on peri-implant bone healing.
- Published
- 2008
- Full Text
- View/download PDF
48. Tenascin and fibronectin expression in carcinoma ex pleomorphic adenoma.
- Author
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Araújo VC, Furuse C, Cury PR, Altemani A, Alves VA, and de Araújo NS
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Fibronectins analysis, Humans, Male, Middle Aged, Tenascin analysis, Adenoma, Pleomorphic metabolism, Fibronectins metabolism, Salivary Gland Neoplasms metabolism, Tenascin metabolism
- Abstract
This study was conducted to analyze the participation of tenascin and fibronectin, components of the extracellular matrix, in different types of carcinoma ex pleomorphic adenoma (CXPA). Seventeen cases of CXPA, classified according to the presence of epithelial and myoepithelial cells and the degree of invasion-intracapsular, minimally, and frankly invasive carcinoma-were immunohistochemically labeled for tenascin and fibronectin. Normal salivary gland included in the specimens showed tenascin only around the excretory duct, and fibronectin slightly expressed all over the stroma of the gland. In reminiscent pleomorphic adenoma, tenascin and fibronectin were observed around tubular structures and in the stroma. Both tenascin and fibronectin were expressed in all the CXPA studied. In areas of in situ carcinoma of the intracapsular type, the expression of these extracellular matrix proteins was enhanced compared with areas of residual pleomorphic adenoma. In intracapsular and minimally invasive types of CXPA, some areas of the tumor border presented tenascin and no fibronectin, pattern that may represent the real invasive front. In frankly invasive CXPA type with only epithelial component, fibronectin was strongly observed in a fibrillar network pattern, and tenascin was only focal. In frankly invasive type with myoepithelial component, tenascin staining was very strong and diffuse. This study showed different patterns of expression of tenascin and fibronectin along the process of tumorigenesis and tumor progression in CXPA, a fact that might play a role in invasion properties of these tumors.
- Published
- 2008
- Full Text
- View/download PDF
49. Desmoplasia in different degrees of invasion of carcinoma ex-pleomorphic adenoma.
- Author
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de Araújo VC, Furuse C, Cury PR, Altemani A, Alves VA, and de Araújo NS
- Subjects
- Actins metabolism, Adenocarcinoma metabolism, Adenoma, Pleomorphic metabolism, Adult, Aged, Aged, 80 and over, Biomarkers, Tumor metabolism, Female, Humans, Male, Middle Aged, Neoplasm Invasiveness, Neoplasms, Multiple Primary, Stromal Cells metabolism, Stromal Cells pathology, Adenocarcinoma pathology, Adenoma, Pleomorphic pathology
- Abstract
Stroma desmoplasia was studied by immunohistochemistry for alpha-smooth muscle actin (alpha-SMA) in 17 instances of carcinoma ex-pleomorphic adenoma (CXPA) classified according to the presence of epithelial and myoepithelial cells and the degree of invasion: intracapsular, minimally and frankly invasive carcinoma. In "resident" pleomorphic adenoma, no desmoplasia was detected. In invasive areas of the intracapsular type of CXPA with only an epithelial component, desmoplasia started to be revealed by the presence of myofibroblasts close to the capsule. In the minimally invasive type, myofibroblasts were seen in the septum between islands of malignant cells and in focal peripheral areas of the tumor interpreted as the actual front of invasion. In the frankly invasive type of CXPA showing large blocks of cells, intense desmoplasia was seen, also separating the tumor cells from the neighboring normal tissue. In tumors with cords and/or small nests of cells, desmoplasia was very slight. In the invasive type of CXPA with a myoepithelial component, alpha-SMA expression was seen in the septum between the islands of cells. The expression was less intense and not present in all areas of the stroma. In CXPA with epithelial and myoepithelial cells, myofibroblasts were rarely seen in the septum separating sheets of cells. Thus, we may deduce that the presence of desmoplasia parallels the capacity of invasion of CXPA by epithelial cells, being minimum in the intracapsular and minimally invasive type of CXPA and increasing as the tumor becomes frankly invasive. Furthermore, we may also conclude that in CXPA with a myoepithelial component, desmoplasia is very rare.
- Published
- 2007
- Full Text
- View/download PDF
50. Malignancy-related 67kDa laminin receptor in adenoid cystic carcinoma. Effect on migration and beta-catenin expression.
- Author
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Morais Freitas V, Nogueira da Gama de Souza L, Cyreno Oliveira E, Furuse C, Cavalcanti de Araújo V, and Gastaldoni Jaeger R
- Subjects
- Antineoplastic Agents pharmacology, Carcinoma, Adenoid Cystic pathology, Cells, Cultured metabolism, Humans, Oligopeptides pharmacology, Salivary Gland Neoplasms pathology, Carcinoma, Adenoid Cystic metabolism, Laminin metabolism, Neoplasm Proteins metabolism, Receptors, Laminin metabolism, Salivary Gland Neoplasms metabolism
- Abstract
Adenoid cystic carcinoma is a malignant salivary gland neoplasm with recurrence and metastasis. We studied the expression of a malignancy-related non-integrin laminin receptor, the 67LR, in this neoplasm. Immunohistochemistry showed 67LR in adenoid cystic carcinoma. This receptor binds a sequence of laminin beta1 chain, the YIGSR peptide. We studied the effect of 67LR and YIGSR in cells (CAC2) from adenoid cystic carcinoma. Three-dimensional cultures of cells embedded into either laminin-111 gel (controls) or YIGSR-enriched laminin-111 (treated) were prepared and studied by light microscopy. CAC2 cells treated with YIGSR appeared fibroblast-like, while control cells were epithelioid. Blockage of 67LR by antibody abolished YIGSR effect in three-dimensional cultures. We analysed the relevance of 67LR and YIGSR on beta-catenin expression in CAC2 cells. Immunofluorescence and immunoblot showed that YIGSR decreased beta-catenin, while blockage of 67LR restored the presence of this molecule. The 67LR and YIGSR induced fibroblast-like morphology in CAC2 cells, with disruption of cell-cell contacts and decrease of beta-catenin. These features resemble epithelial-mesenchymal transition (EMT). EMT also increases cell migration. In monolayer assays YIGSR increased migration of CAC2 cells. We conclude that 67LR and YIGSR are involved in epithelial-mesenchymal transition, modulation of beta-catenin expression, and migratory activity of CAC2 cells.
- Published
- 2007
- Full Text
- View/download PDF
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