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2. Hydroxychloroquine attenuates cigarette smoke induced autophagic signaling in the mouse ovary.

Author

Furlong HC, Wessels JM, Guerra MT, Stämpfli MR, and Foster WG

Subjects
Animals, Autophagosomes drug effects, Autophagy drug effects, Autophagy physiology, Beclin-1 genetics, Beclin-1 metabolism, Body Weight drug effects, Female, Mice, Inbred C57BL, Microscopy, Electron, Transmission, Microtubule-Associated Proteins genetics, Microtubule-Associated Proteins metabolism, Ovary metabolism, Ovary ultrastructure, Signal Transduction drug effects, Hydroxychloroquine pharmacology, Ovary drug effects, Protective Agents pharmacology, Smoke adverse effects, Tobacco Products adverse effects
Abstract

We previously demonstrated that Cigarette Smoke (CS) induces autophagy in the ovary. Therefore we aimed to determine if chloroquine (CQ) could inhibit CS-induced autophagy in the ovary. Eight week old mice were implanted with CQ pellets; 0, 25, and 50mg CQ/kg. Half of the animals in each group were exposed to room air and the other half were exposed to CS twice daily for 8 weeks. Ovaries were harvested for electron microscopy, gene and protein expression analysis. There was a significant increase in the production of autophagosomes in granulosa cells of mice exposed to CS (p=0.0297). However 25 and 50mg/kg CQ treatment significantly decreased the CS-induced autophagosomes (p=0.0505; p=0.0065) and attenuated the effects of CS on LC3B and BECN1 expression. In summary, this suggests that CQ attenuates CS-induced autophagy in the ovary and that ovarian protection from toxic insult is potentially feasible., (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)

Published
2016
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