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144 results on '"Funes, Soledad"'

1. Prohibitins, Phb1 and Phb2, function as Atg8 receptors to support yeast mitophagy and also play a negative regulatory role in Atg32 processing.

Author

García-Chávez, Diana, Domínguez-Martín, Eunice, Kawasaki, Laura, Ongay-Larios, Laura, Ruelas-Ramírez, Hilario, Mendoza-Martinez, Ariann E., Pardo, Juan P., Funes, Soledad, and Coria, Roberto

Subjects
GREEN fluorescent protein, TUBULINS, N-terminal residues, SODIUM dodecyl sulfate, SCAFFOLD proteins, UBIQUITIN ligases
Abstract

The prohibitins Phb1 and Phb2 assemble at the mitochondrial inner membrane to form a multi-dimeric complex. These scaffold proteins are highly conserved in eukaryotic cells, from yeast to mammals, and have been implicated in a variety of mitochondrial functions including aging, proliferation, and degenerative and metabolic diseases. In mammals, PHB2 regulates PINK1-PRKN mediated mitophagy by interacting with lipidated MAP1LC3B/LC3B. Despite their high conservation, prohibitins have not been linked to mitophagy in budding yeasts. In this study, we demonstrate that both Phb1 and Phb2 are required to sustain mitophagy in Saccharomyces cerevisiae. Prohibitin-dependent mitophagy requires formation of the Phb1-Phb2 complex and a conserved AIM/LIR-like motif identified in both yeast prohibitins. Furthermore, both Phb1 and Phb2 interact and exhibit mitochondrial colocalization with Atg8. Interestingly, we detected a basal C terminus processing of the mitophagy receptor Atg32 that depends on the presence of the i-AAA Yme1. In the absence of prohibitins this processing is highly enhanced but reverted by the inactivation of the rhomboid protease Pcp1. Together our results revealed a novel role of yeast prohibitins in mitophagy through its interaction with Atg8 and regulating an Atg32 proteolytic event. Abbreviation: AIM/LIR: Atg8-family interacting motif/LC3-interacting region; ANOVA: analysis of variance; ATG/Atg: autophagy related; C terminus/C-terminal: carboxyl terminus/carboxyl-terminal; GFP: green fluorescent protein; HA: human influenza hemagglutinin; Idh1: isocitrate dehydrogenase 1; MAP1C3B/LC3B: microtubule associated protein 1 light chain 3 beta; mCh: mCherry; MIM: mitochondrial inner membrane; MOM: mitochondrial outer membrane; N starvation: nitrogen starvation; N terminus: amino terminus; PARL: presenilin associated rhomboid like; Pcp1: processing of cytochrome c peroxidase 1; PCR: polymerase chain reaction; PGAM5: PGAM family member 5 mitochondrial serine/threonine protein phosphatase; PHBs/Phb: prohibitins; PINK1: PTEN induced kinase 1; PMSF: phenylmethylsulfonyl fluoride; PRKN: parkin RBR E3 ubiquitin protein ligase; SD: synthetic defined medium; SDS: sodium dodecyl sulfate; SMD-N: synthetic defined medium lacking nitrogen; WB: western blot; WT: wild type; Yme1: yeast mitochondrial escape 1; YPD: yeast extract-peptone-dextrose medium; YPLac: yeast extract-peptone-lactate medium. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Autophagy in Stationary Phase of Growth

Author

Aguilar-López, José L., Funes, Soledad, Timmis, Kenneth N., Editor-in-Chief, Boll, Matthias, Series Editor, Geiger, Otto, Series Editor, Goldfine, Howard, Series Editor, Krell, Tino, Series Editor, Lee, Sang Yup, Series Editor, McGenity, Terry J., Series Editor, Rojo, Fernando, Series Editor, Sousa, Diana Z, Series Editor, Stams, Alfons J. M., Series Editor, Steffan, Robert, Series Editor, and Wilkes, Heinz, Series Editor

Published
2019
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4. The Apicoplast

Author

Funes, Soledad, Pérez-Martínez, Xochitl, Reyes-Prieto, Adri án, González-Halphen, Diego, Jee, Govind, editor, Wise, Robert R., editor, and Hoober, J. Kenneth, editor

Published
2006
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10. Viaje al centro de la mitocondria: importación de proteínas, sus alteraciones y enfermedades relacionadas

Author

Avendaño Monsalve, Maria Clara, Ponce Rojas, José Carlos, Funes, Soledad, Avendaño Monsalve, Maria Clara, Ponce Rojas, José Carlos, and Funes, Soledad

Abstract

Las mitocondrias son organelos fascinantes, no solo porque son el sitio en donde se genera la energía de las células, sino por su relevancia en procesos y patologías de interés médico. La gran mayoría de las proteínas que constituyen el proteoma mitocondrial están codificadas en el núcleo y se traducen por ribosomas citosólicos, por lo que deben ser identificadas correctamente para ser distribuidas e insertadas en cada uno de los subcompartimentos mitocondriales. En este artículo realizamos una descripción de las las maquinarias de importación mitocondrial, además de las diferentes respuestas celulares que contrarrestan las alteraciones en los procesos de transporte de las proteínas o cuando existe una disfunción mitocondrial. Finalmente, mencionamos las enfermedades causadas por mutaciones en los complejos transportadores y de distribución de las proteínas de este organelo, que se han identificado hasta la fecha., Mitochondria are exciting organelles not only because they are in charge of the energy production within the cell, but also because of their relevance in processes and pathologies of medical interest. The vast majority of the proteins that constitute the mitochondrial proteome are encoded in the nucleus and translated by cytosolic ribosomes, hence these have to be correctly identified in order to be distributed and inserted in each of the mitochondrial subcompartments. In this review, we describe the mitochondrial import machineries, as well as the different cellular responses to alterations in protein transport processes or when a mitochondrial dysfunction arises. Lastly, we give an overview of the identified pathologies triggered by mitochondrial dysfunction derived from mutations in the components of the import and sorting machineries of the organelle.

Published
2021

11. Reconstructing the mitochondrial protein import machinery of Chlamydomonas reinhardtii

Author

Figueroa-Martinez, Francisco, Funes, Soledad, Franzen, Lars-Gunnar, and Gonzalez-Halphen, Diego

Subjects
Chlamydomonas -- Research, Chlamydomonas -- Genetic aspects, Cellular proteins -- Research, Oxidative phosphorylation -- Research, Biological sciences
Abstract

In Chlamydomonas reinhardtii several nucleus-encoded proteins that participate in the mitochondrial oxidative phosphorylation are targeted to the organelle by unusually long mitochondrial targeting sequences. Here, we explored the components of the mitochondrial import machinery of the green alga. We mined the algal genome, searching for yeast and plant homologs, and reconstructed the mitochondrial import machinery. All the main translocation components were identified in Chlamydomonas as well as in Arabidopsis thaliana and in the recently sequenced moss Physcomitrella patens. Some of these components appear to be duplicated, as is the case of Tim22. In contrast, several yeast components that have relatively large hydrophilic regions exposed to the cytosol or to the intermembrane space seem to be absent in land plants and green algae. If present at all, these componentes of plants and algae may differ significantly from their yeast counterparts. We propose that long mitochondrial targeting sequences in some Chlamydomonas mitochondrial protein precursors are involved in preventing the aggregation of the hydrophobic proteins they carry.

Published
2008

18. The Apicoplast

Author

Funes, Soledad, primary, Pérez-Martínez, Xochitl, additional, Reyes-Prieto, Adri án, additional, and González-Halphen, Diego, additional

Published
2007
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22. A Green Algal Apicoplast Ancestor

Author

Funes, Soledad, Davidson, Edgar, Reyes-Prieto, Adrián, Magallón, Susana, Herion, Pascal, King, Michael P., and González-Halphen, Diego

Published
2002

23. Viaje al centro de la mitocondria: importación de proteínas, sus alteraciones y enfermedades relacionadas.

Author

Clara Avendaño-Monsalve, Maria, Carlos Ponce-Rojas, José, and Funes, Soledad

Subjects
CARRIER proteins, PROTEIN transport, MITOCHONDRIAL pathology, MITOCHONDRIA, PROTEINS, ORGANELLES, RIBOSOMES
Abstract

Copyright of TIP Revista Especializada en Ciencias Químico-Biológicas is the property of Universidad Nacional Autonoma de Mexico, Facultad de Estudios Superiores Zaragoza and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2021
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26. The Apicoplast

Author

Funes, Soledad, primary, Pérez-Martínez, Xochitl, additional, Reyes-Prieto, Adrián, additional, and González-Halphen, Diego, additional

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27. Transfer of mitochondrial genes to the nucleus in chlamydomonad algae: perspective for the allotopic expression of OX-PHOS proteins and future human therapies

Author

Funes, Soledad, Perez-Martinez, Xochitl, Antaramian, Anaid, Vazquez-Acevedo, Miriam, VAN LIS, Robert, Reyes-Prieto, Adrian, Santillan-Torres, Jose Luis, Gonzalo Claros, M, Davidson, Edgar, King, Michael P, Gonzalez-Halphen, Diego, Departamento de Genética Molecular, Instituto de Fisiologıa Celular, and National Autonomous University of Mexico

Subjects
[SDV]Life Sciences [q-bio]
Abstract

Transfer of mitochondrial genes to the nucleus in chlamydomonad algae: perspective for the allotopic expression of OX-PHOS proteins and future human therapies

Published
2002

28. Chlamydomonas reinhardtii : the model of choice to study mitochondria from unicellular photosynthetic organisms.

Author

Funes, Soledad, Franzén, Lars-Gunnar, González-Halphen, Diego, Funes, Soledad, Franzén, Lars-Gunnar, and González-Halphen, Diego

Abstract

Chlamydomonas reinhardtii is a model organism to study photosynthesis, cellular division, flagellar biogenesis, and, more recently, mitochondrial function. It has distinct advantages in comparison to higher plants because it is unicellular, haploid, and amenable to tetrad analysis, and its three genomes are subject to specific transformation. It also has the possibility to grow either photoautotrophically or heterotrophically on acetate, making the assembly of the photosynthetic machinery not essential for cell viability. Methods developed allow the isolation of C. reinhardtii mitochondria free of thylakoid contaminants. We review the general procedures used for the biochemical characterization of mitochondria from this green alga., Ingår i: MitochondriaPractical Protocols, ISBN: 978-1-58829-667-2 (Print) 978-1-59745-365-3 (Online)

Published
2007
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29. Partial suppression of Oxa1 mutants by mitochondria-targeted signal recognition particle provides insights into the evolution of the cotranslational insertion systems

Author

Funes, Soledad, primary, Westerburg, Heike, additional, Jaimes-Miranda, Fabiola, additional, Woellhaf, Michael W., additional, Aguilar-Lopez, Jose L., additional, Janßen, Linda, additional, Bonnefoy, Nathalie, additional, Kauff, Frank, additional, and Herrmann, Johannes M., additional

Published
2012
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44. Analysis of Mitochondrial Protein Synthesis in Yeast.

Author

Walker, John M., Leister, Dario, Funes, Soledad, and Herrmann, Johannes M.

Abstract

Mitochondrial biogenesis is an intricate process that requires the coordinated function of two separate genetic systems: one in the organelle and one in the nucleus. The study of mitochondria requires the analysis of both genetic systems and their protein products. We describe the general procedures used to label mitochondrially encoded proteins in the baker's yeast Saccharomyces cerevisiae, a starting point for the investigation of various aspects of organelle biogenesis, such as folding and assembly, sorting, and degradation of proteins. [ABSTRACT FROM AUTHOR]

Published
2007
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45. Chlamydomonas reinhardtii: The Model of Choice to Study Mitochondria From Unicellular Photosynthetic Organisms.

Author

Walker, John M., Leister, Dario, Herrmann, Johannes M., Funes, Soledad, Franzén, Lars-Gunnar, and González-Halphen, Diego

Abstract

Chlamydomonas reinhardtii is a model organism to study photosynthesis, cellular division, flagellar biogenesis, and, more recently, mitochondrial function. It has distinct advantages in comparison to higher plants because it is unicellular, haploid, and amenable to tetrad analysis, and its three genomes are subject to specific transformation. It also has the possibility to grow either photoautotrophically or heterotrophically on acetate, making the assembly of the photosynthetic machinery not essential for cell viability. Methods developed allow the isolation of C. reinhardtii mitochondria free of thylakoid contaminants. We review the general procedures used for the biochemical characterization of mitochondria from this green alga. [ABSTRACT FROM AUTHOR]

Published
2007
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46. The Apicoplast.

Author

GOVINDJEE, Wise, Robert R., Hoober, J. Kenneth, Funes, Soledad, Pérez-Martínez, Xochitl, Reyes-Prieto, Adrián, and González-Halphen, Diego

Abstract

The apicoplast is an essential organelle characteristic of the apicomplexan parasites. It harbors its own genome and it is believed to be a chloroplast-derived organelle that originated by secondary endosymbiosis. Here, we address the more relevant properties of this organelle, an evolutionary relict of a once fully-functional algal chloroplast. We address how its highly-reduced plastid genome replicates and segregates, and how it gets transcribed and translated. We also describe the particular metabolism of the apicoplast, limited to certain pathways, including fatty acid and lipid biosynthesis, the non-mevalonate isoprenoid synthesis pathway, the biosynthesis of iron-sulfur clusters, and the de novo synthesis of heme groups. These metabolic pathways are of relevance as a preferred target for anti-parasitic drugs. The organelle also exhibits peculiar protein-import mechanisms. Numerous genes encoding apicoplast proteins are located in the nucleus, and the encoded proteins are targeted to the organelle in a process mediated by bipartite N-terminal extensions present in the protein precursors. The nature of the alga that was phagocytized and retained by the apicomplexan ancestor through a secondary endosymbiotic event remains controversial. We critically address the obscure evolutionary origin of the apicoplast and we review the evidence that has given rise to the so-called rhodophyte versus chlorophyte debate. The apicoplast is a fascinating organelle that has attracted many research groups in the last years. Our current knowledge about it its certainly limited, and its study is essential to understand the physiology and evolution of the apicomplexan parasites. [ABSTRACT FROM AUTHOR]

Published
2006
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49. Unusual Location of a Mitochondrial Gene

Author

Pérez-Martı́nez, Xochitl, primary, Vázquez-Acevedo, Miriam, additional, Tolkunova, Elena, additional, Funes, Soledad, additional, Claros, Manuel G., additional, Davidson, Edgar, additional, King, Michael P., additional, and González-Halphen, Diego, additional

Published
2000
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