1. fcsA29Mutation is an Allele ofpolAGene ofEscherichia coli
- Author
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Kazuo Nagai, Takashi Hirasawa, Ayako Takada, Kohji Nagano, Masaaki Wachi, and Fumie Takaku
- Subjects
Exonuclease ,Cell Survival ,Ultraviolet Rays ,Mutant ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Biochemistry ,Analytical Chemistry ,Escherichia coli ,medicine ,Point Mutation ,Allele ,SOS response ,SOS Response, Genetics ,Molecular Biology ,Gene ,Alleles ,Genetics ,biology ,Genetic Complementation Test ,Organic Chemistry ,General Medicine ,DNA Polymerase I ,Molecular biology ,Cold Temperature ,Rec A Recombinases ,Mutation (genetic algorithm) ,biology.protein ,bacteria ,DNA polymerase I ,Biotechnology - Abstract
The cold-sensitive fcsA29 mutation of Escherichia coli was found to be a new type of cold-sensitive allele of the polA gene encoding DNA polymerase I, caused by an Asp(116)--Asn change in the 5'--3' exonuclease domain. The fcsA29 mutant showed typical polA mutant phenotypes such as UV sensitivity and unacceptability of recA mutation. Cold-sensitive growth of the mutant was suppressed by introduction of a sulA mutation, indicating that cell filamentation was due to the SOS response.
- Published
- 1999
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