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Introduction: In special populations, such as Reserve Officers' Training Corps (ROTC) cadets, body composition is used not only as a predictor of fitness but for additional purposes such as qualification for enlistment, load carriage, and duty fulfillment. Body mass index (BMI) is the initial measurement recorded and is considered a representation of health and physical performance capabilities. Personnel exceeding threshold values of body weight based upon their height measurement are typically further evaluated using a circumference-based method that predicts the cadets' percent body fat. Military personnel who fail to meet these body composition standards may be penalized by being denied specific positions or promotions or risk being relieved from all military duties. In order to differentiate and accurately assess the components that constitute body composition, other methods of measuring body composition that provide greater accuracy should be explored. The purpose of this study was to compare multiple body composition methods, including the military's method of circumference-based measurement, in order to identify a suitable method for Air Force ROTC programs., Materials and Methods: Participants were recruited from an Air Force ROTC Detachment at a large university in the Midwest United States. Anthropometric (height and weight) and body composition measurements (air displacement plethysmography [ADP], bioelectrical impedance analysis, skinfolds, and circumferences) were collected for each participant. A repeated-measure analysis of variance was used to compare body composition measurement methods. A Bonferroni adjustment was utilized for multiple comparisons. BMI and circumference results were displayed as a percentage of compliance according to Air Force Instruction guidelines. The university institutional review board approval was established to ensure that the design of this study protected the rights of the participants., Results: Twenty-four (21 males and 3 females) participants completed the study. A significant difference between skinfolds and bioelectrical impedance analysis occurred (P = .025). There were no other significant differences identified between other methods of body composition. Using the BMI and circumference compliant/non-compliant scale listed in the Air Force Instruction guidelines, a greater number of cadets fell into the non-compliant category according to BMI (n = 7) versus circumferences (n = 1). The circumference-based method underestimated body fat compared to the "gold standard" ADP., Conclusions: The findings from this investigation suggest that the circumference-based method can appropriately provide accurate body composition results among Air Force ROTC cadets. Results also determined that the military's circumference-based method underestimated body fat compared to the "gold standard" ADP. Although circumference-based measurements might be efficient for use in larger populations, Air Force ROTC programs should evaluate other methods of measuring body composition to best fit the needs of individual cadets. Further research should be conducted to identify body composition methods that are easy to implement and provide accurate results at the individual level., (© The Association of Military Surgeons of the United States 2023. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Purpose: The purpose of this investigation was to examine the impact of instruction order on the speech production response when adopting higher effort speaking styles, specifically loud and clear speech., Method: Speech intensity, lip aperture range, and speech rate data were collected from 24 talkers who repeated the utterance "Buy Bobby a puppy" using habitual, clear, and loud speech. Participants were assigned in quasi-random fashion to one of two groups: a Clear-Loud Group (11 participants; order: habitual-clear-loud) or a Loud-Clear Group (13 participants; order: habitual-loud-clear)., Results: Talkers in the Clear-Loud Group exhibited higher speech intensity during the loud style compared with those who performed the Loud-Clear Group. Furthermore, talkers in the Clear-Loud Group retained the increases lip aperture range and reductions in speech rate associated with the clear style when producing the loud style. Conversely, talkers in the Loud-Clear Group exhibited significant increases in lip aperture range between the habitual and loud styles and between the loud and clear styles. Additionally, the Loud-Clear Group exhibited a reduction in speech rate only during the clear style, as no differences in speech rate were observed between the habitual and loud styles., Conclusions: These findings may suggest that producing a higher effort style leads to carry-over effects in subsequent styles. Future research should investigate factors that moderate the degree of order effects for both research and clinical purposes. For instance, if generalizable, the compounding carry-over effects may prove advantageous for certain clinical populations.

Purpose: The purpose of this research note was to quantify the impact of concurrent performance of an attention-demanding secondary task on utterance-to-utterance movement variability associated with higher effort speaking styles, namely, clear and loud speech., Method: Lip and jaw kinematics collected as part of a prior study were analyzed. Participants repeated "Buy Bobby a puppy" using habitual, loud, and clear speech styles in isolation and while performing a secondary tracking task. The lip aperture (LA) signal was segmented based on opening and closing gestures associated with the utterance. The standard deviation of each segment duration was calculated to quantify temporal variability. To quantify spatial variability, each segment was first time normalized. The mean standard deviation of the overlapping time-normalized LA amplitudes was computed for each segment from the repetitions produced in each speech style and condition (speaking in isolation vs. speaking while tracking). A relative measure of spatial variation was also computed to account for the potential impact of articulatory scaling., Results: Clear speech was associated with greater temporal and spatial variability than the habitual and loud styles. In the habitual style, talkers also exhibited a slight reduction in absolute spatial variability when speaking while tracking compared to speaking in isolation. The reduction in absolute spatial variability was likely associated with the concomitant reduction in LA range of motion, as there was no change in the relative spatial variability between conditions., Conclusion: The current investigation expands prior work by quantifying spatial and temporal characteristics of different speaking styles performed in isolation and while concurrently performing an attention-demanding visuomotor task.

Purpose Prior work has demonstrated that competing tasks impact habitual speech production. The purpose of this investigation was to quantify the extent to which clear and loud speech are affected by concurrent performance of an attention-demanding task. Method Speech kinematics and acoustics were collected while participants spoke using habitual, loud, and clear speech styles. The styles were performed in isolation and while performing a secondary tracking task. Results Compared to the habitual style, speakers exhibited expected increases in lip aperture range of motion and speech intensity for the clear and loud styles. During concurrent visuomotor tracking, there was a decrease in lip aperture range of motion and speech intensity for the habitual style. Tracking performance during habitual speech did not differ from single-task tracking. For loud and clear speech, speakers retained the gains in speech intensity and range of motion, respectively, while concurrently tracking. A reduction in tracking performance was observed during concurrent loud and clear speech, compared to tracking alone. Conclusions These data suggest that loud and clear speech may help to mitigate motor interference associated with concurrent performance of an attention-demanding task. Additionally, reductions in tracking accuracy observed during concurrent loud and clear speech may suggest that these higher effort speaking styles require greater attentional resources than habitual speech.

Purpose Prior investigations suggest that simultaneous performance of more than 1 motor-oriented task may exacerbate speech motor deficits in individuals with Parkinson disease (PD). The purpose of the current investigation was to examine the extent to which performing a low-demand manual task affected the connected speech in individuals with and without PD. Method Individuals with PD and neurologically healthy controls performed speech tasks (reading and extemporaneous speech tasks) and an oscillatory manual task (a counterclockwise circle-drawing task) in isolation (single-task condition) and concurrently (dual-task condition). Results Relative to speech task performance, no changes in speech acoustics were observed for either group when the low-demand motor task was performed with the concurrent reading tasks. Speakers with PD exhibited a significant decrease in pause duration between the single-task (speech only) and dual-task conditions for the extemporaneous speech task, whereas control participants did not exhibit changes in any speech production variable between the single- and dual-task conditions. Conclusions Overall, there were little to no changes in speech production when a low-demand oscillatory motor task was performed with concurrent reading. For the extemporaneous task, however, individuals with PD exhibited significant changes when the speech and manual tasks were performed concurrently, a pattern that was not observed for control speakers. Supplemental Material https://doi.org/10.23641/asha.8637008.

Sensing carbohydrates via the oral cavity benefits performance outcomes during brief high intensity bouts of exercise. However, the extent to which carbohydrates need to be present in the oral cavity to influence sprint performance is less understood. The purpose of this study was to determine if serial increases in carbohydrate rinse time across sprint sets attenuates increases in sprint time compared to no serial increases in carbohydrate rinse time across sprint sets. Fifteen sprint trained participants completed three repeated anaerobic sprint tests (RAST), 3 sets of 6 x 35-m sprints, under two different carbohydrate mouth rinsing (CMR) conditions; (1) rinsing for only 5 seconds (s), and (2) rinsing for 5 s, 10 s and 15 s (serial rinse). Prior to a RAST, participants provided perceived recovery status (PRS) and perceived feeling of arousal (FAS). Upon completion of each individual sprint, participants gave a rating of perceived exertion (RPE). A lactate sample was taken upon completion of each individual sprint set and after all 3 RASTs a session rating of perceived exertion (S-RPE) was measured. There were no significant differences in peak (p = 0.18) and average sprint time (p = 0.41). There were no significant differences in perceptual measures: RPE, PRS, FAS, S-RPE or for blood lactate concentration between CMR conditions. Overall, serial rinsing resulted in changes that were most likely trivial, but showed a 50% chance in perceiving a sprint session as less difficult. Rinsing carbohydrates in a serial manner between repeated sprint sets produces trivial changes of sprint speed and perceptual measures from sprint performance.

Context: Motorized treadmills (MTs) present an altered motor task compared to overground (OG) locomotion in that MT belt surfaces are motor-driven, whereas individuals walking/running OG must propel themselves. A possible solution may lie with novel nonmotorized treadmill (NMT) devices as the belt surface is propelled by the user., Objective: The purpose of this study was to compare gait performance during both MT and NMT locomotion to OG., Design: Crossover study., Setting: A university research laboratory., Patients: A total of 20 healthy adults (10 women) participated in the study., Intervention: Each participant performed self-selected walking and running OG, and on both an MT and NMT., Main Outcome Measure: Shoulder, trunk, and lower-extremity kinematics were analyzed for each treadmill condition and compared to OG., Results: The analyses demonstrated that there were no differences between MT and OG gait kinematics during either walking or running. However, NMT gait showed increased hip, knee, and ankle flexions in late swing and early stance compared to OG during both walking and running. For example, during walking, the NMT elicited hip-, knee-, and ankle-flexion/extension angles of 34.7°, 8.0°, and 3.6° at foot strike compared to 24.8°, -3.1°, and -5.8° in the OG condition (P < .05). There was also a significant reduction in trunk-flexion/extension range of motion during running compared to OG (7.7° in NMT vs 9.8° in OG)., Conclusions: These differences may have implications for both training and rehabilitation on an NMT. Future studies should consider the influence of NMT familiarization on gait performance and should emphasize the assessment of neuromuscular performance.

Morgan, AL, Laurent, CM, and Fullenkamp, AM. Comparison of V[Combining Dot Above]O2peak performance on a motorized vs. a nonmotorized treadmill. J Strength Cond Res 30(7): 1898-1905, 2016-Despite growing popularity of nonmotorized treadmills (NMTs), little data exist regarding responses during exercise testing using this equipment, which is important when providing an appropriate exercise prescription. The purpose of this study was to evaluate physiological and perceptual responses during peak graded exercise tests (GXTs) on a motorized treadmill (MT) vs. NMT. Volunteers (12 men and 12 women aged 18-35 years) performed 2 peak GXT sessions (1 MT and 1 NMT). Respiratory gases and heart rate (HR) were collected each minute; perceptual response was estimated (Borg's 6-20 rating of perceived exertion [RPE] scale) during the final 10 seconds of each stage. Peak values (i.e., V[Combining Dot Above]O2, HR, speed) were determined during the final 10 seconds of each test; ventilatory threshold (VT) was assessed using the V-slope method. Paired t-tests matching variables measured at each stage of the GXT identified significantly higher values on the NMT for V[Combining Dot Above]O2 83% of the time, HR 67% of the time, and RPE 25% of the time. Interestingly though, neither peak V[Combining Dot Above]O2 (48.6 ± 9.2 ml·kg·min vs. 47.8 ± 8.9 ml·kg·min), peak HR (185 ± 9 b·min vs. 188 ± 10 b·min; p = 0.90), nor VT (72.7 ± 5.7% vs. 73.8 ± 5.4%) were significantly different on the NMT vs. the MT. However, significant differences were identified between NMT and MT tests for time to exhaustion (9:55 ± 1:49 vs. 12:05 ± 2:48; p < 0.01) and peak speed (8.0 ± 0.9 mph vs. 9.2 ± 1.4 mph; p < 0.01). Thus, although peak values obtained were similar between testing sessions on the NMT and MT, the majority of submaximal data were significantly different between trials. These differences are important when designing exercise prescriptions using submaximal values from NMT testing that may be inappropriately high or low at corresponding intensities during training.

To date, biomechanical analyses of soccer kicking have focused predominantly on lower-extremity motions, with little emphasis on the trunk and upper body. The purpose of this study was to evaluate differences in trunk axial kinematics between novice (n = 10) and skilled (n = 10) participants, as well as to establish the relationship of trunk axial motion and sagittal plane thigh rotation to poststrike ball velocity. Three-dimensional body segmental motion data were captured using high-resolution motion analysis (120 Hz) while each participant completed 5 maximal instep soccer-style kicks. The results demonstrate that skilled participants use 53% greater axial trunk range of motion compared with novice participants (P < .01), as well as 62% greater peak trunk rotation velocity (P < .01). The results also show a moderate, positive correlation of peak trunk rotation velocity with poststrike ball velocity (r = .57; P < .01), and peak hip flexion velocity with poststrike ball velocity (r = .63; P < .01). The current study highlights the potential for trunk rotation-specific training to improve maximum instep kick velocity in developing soccer athletes.

Metabolic syndrome (MetS) doubles the risk of adverse cardiovascular events. Glucagon-like peptide 1 (GLP-1) receptor agonists induce weight loss, increase insulin secretion, and improve glucose tolerance. Studies in healthy animals suggest cardioprotective properties of GLP-1 receptor agonists, perhaps partially mediated by improved sarco-endoplasmic reticulum Ca(2+) ATPase (SERCA) activity. We examined the acute effect of GLP-1 receptor agonists on coronary smooth muscle cells (CSM) enzymatically isolated from lean, healthy Ossabaw miniature swine. Intracellular Ca(2+) handling was interrogated with fura-2. The GLP-1 receptor agonist exenatide activated SERCA but did not alter other Ca(2+) transporters. Further, we tested the hypothesis that chronic, in vivo treatment with GLP-1 receptor agonist AC3174 would attenuate coronary artery disease (CAD) in swine with MetS. MetS was induced in 20 swine by 6 months' feeding of a hypercaloric, atherogenic diet. Swine were then randomized (n = 10/group) into placebo or AC3174 treatment groups and continued the diet for an additional 6 months. AC3174 treatment attenuated weight gain, increased insulin secretion, and improved glucose tolerance. Intravascular ultrasound and histology showed no effect of AC3174 on CAD. MetS abolished SERCA activation by GLP-1 receptor agonists. We conclude that MetS confers vascular resistance to GLP-1 receptor agonists, partially through impaired cellular signaling steps involving SERCA., (© 2015 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.)

Despite the available literature addressing the placebo effect's role in mediating human performance, there is a paucity of research addressing the possibility of a placebo effect both within and between bouts of repeated sprint performance on consecutive days. Therefore, the purpose of this study was to determine whether the administration of a placebo influences recovery during sessions of intermittent sprinting. Ten subjects performed 4 repeated sprint tests under 2 different conditions; 2 while being administered a control beverage separated by 24 hours of recovery and the other 2 with a placebo beverage separated by 24 hours of recovery. Before each sprint test, subjects provided perceived recovery status (PRS). Ratings of perceived exertion were recorded within 5 seconds after each sprint. After each repeated sprint protocol, subjects were asked to provide a rating of perceived exertion (RPE), rate their pain, and provided a blood lactate sample. Power was recorded throughout each session from a nonmotorized treadmill to analyze changes in sprinting performance. Repeated-measures analysis of variance was used to determine significant differences in peak and mean power, PRS, RPE, pain, and blood lactate. The placebo trial produced significantly higher peak (p < 0.001) and mean power (p = 0.002) vs. the control in later sprints absent of any other significant difference in metabolic or perceptual strain (p > 0.05). In conclusion, it seems that the administration of a placebo can attenuate the decline in performance as fatigue increases during repeated sprinting bouts.

Studies on human perception have identified pelvis and torso motion as key discriminators between male and female gaits. However, while most observers would advocate that men and women walk differently, consistent findings and explanations of sex differences in gait kinematics across modern empirical studies are rare. In the present study we evaluated sex differences in whole body gait kinematics from a large sample of subjects (55 men, 36 women) walking at self selected speeds. We analyzed the data through comparisons of discrete metrics and whole curve analyses. Results showed that in the frontal plane, women walked with greater pelvic obliquity than men, but exhibited a more stable torso and head. Women had greater transverse plane pelvis and torso rotation as well as greater arm swing. Additional sex differences were noted at the hip and ankle. These kinematic results are in line with anectdotal observations and qualitative studies. In order to understand these observations and substantiate some of the explanations previously set forth in the biomechanics literature, we also explored possible reasons for dynamic sex effects, and suggested applications that may benefit from their consideration., (Published by Elsevier B.V.)

Non-motorized treadmills (NMT) provide belt speed data that can be used to estimate work output, and potentially, gait temporal-spatial parameters that provide an improved understanding of gait performance. The purpose of this study was to determine the validity of an automated technique that uses belt speed data from an NMT to estimate temporal-spatial gait parameters. Seventeen injury-free adult participants performed a series of 20-s, metronome-guided walking and running trials for each of eight predetermined cadence conditions (72-200 steps/min). Two NMT-based cadence algorithms [PSD estimated cadence (PEC) and threshold estimated cadence (TEC)], and one NMT-based step length algorithm (NMT&#95;SL) were evaluated for their ability to predict traditional motion analysis-based measures of cadence and step length (MAC and MA&#95;SL, respectively). The results of this study demonstrate that both the PEC and TEC algorithms were capable of predicting MAC with a standard error of the estimate (SEE) less than four steps/min (R(2) = 0.997 and R(2) = 0.993, respectively). Predictions of MA&#95;SL from NMT&#95;SL were separated by gait type (walking vs. running) to account for an obvious separation in the step length data with a qualitative gait change. When applied to walking data, NMT&#95;SL was capable of predicting MA&#95;SL with an SEE of 23 mm (R(2) = 0.96). When applied to running data, NMT&#95;SL was capable of predicting MA&#95;SL with an SEE of 44 mm (R(2) = 0.80). The assessment of the novel technique suggests that it is feasible to use non-motorized treadmill belt speed data to predict gait events and analyze simple gait metrics. Future research should evaluate the applicability of these algorithms for use with participants/patients presenting with pathological gait., (Copyright © 2014 Elsevier B.V. All rights reserved.)

This study aimed to evaluate changes in pre- to postseason power output, fatigue, and recovery during a repeated sprint test. Twenty National Collegiate Athletic Association Division I men's hockey athletes performed identical sessions of repeated sprint work pre- and postseason. The repeated sprint test consisted of 5 sets of 45 seconds of repeated sprint work with 90 seconds of rest in between each series of sprints. Power output (W), decrement, and recovery scores (RECs) were determined using raw data from a nonmotorized treadmill. Ratings of perceived exertion were recorded after, and perceived readiness (PR) recorded before, each series of sprints. Mean power was significantly higher in preseason vs. postseason performance during sprint 1 (760.6 vs. 691.3 W; p = 0.03), sprint 2 (719.9 vs 657.0 W; p = 0.05), sprint 4 (648.4 vs 588.9 W; p = 0.04), and sprint 5 (656.6 vs. 586.8 W, p = 0.04). Ratings of perceived exertion were significantly higher during sprints 3, 4, and 5 postseason with PR significantly higher (indicating less readiness) before sprints 3 and 4. There were no significant differences in REC or decrement score. Overall, athletes were unable to maintain power during subsequent repeated sprint work during postseason. The degree to which the athletes fatigued and recovered between sprints did not change between pre- and postseason testing, however, athletes exhibit increased perceptual strain during the repeated sprint work. These data indicate meaningful performance and perceptual differences throughout the competitive season in collegiate-level hockey players.

Recently, there has been a growth in the popularity of resistance exercises performed on unstable surfaces. However, the relationship between unstable surface training and load coupling on muscle activation is unclear. The purpose of this study was to evaluate changes in muscle activation during a barbell (BB) (coupled) and dumbbell (DB) (uncoupled) chest press exercise performed on an unstable surface. The 3 specific chest press conditions included 50% 1 repetition maximum (RM) with BB (50% BB), 50% 1RM with DBs (50% DB), and 25% 1RM with DBs (25% DB). Ten male subjects participated in the study (age, 23.9 ± 2.6 years; body weight, 82.8 ± 10.2 kg). During testing, mean electromyographic activity was assessed for pectoralis major (PM), triceps brachii, anterior deltoid (AD), and rectus abdominis (RA) and was presented as a percent change across the lifting conditions. It was observed that muscle activation increased by 15% in both the PM and RA from the 50% BB condition to the 50% DB condition. Also, the greatest percent difference in muscle activation between the 50 and 25% DB conditions occurred for PM and AD (+54% during 50% DB). These results suggest that demands on the core musculature to provide stability are increased with the use of DBs (uncoupled) as opposed to a BB (coupled). Where instability training provides a sufficient hypertrophy stimulus in prime mover muscle groups, there may be the added benefit of core stability training. Specifically, this type of training may benefit both untrained persons and those engaged in active rehabilitation.

Objective: Obesity is a factor in the outcome and severity of pancreatic conditions. We examined the effect of hypercaloric diets on the pancreata of Ossabaw swine, a large animal model of metabolic syndrome and obesity., Methods: Swine were fed with 1 of 4 diets: high-fructose (n = 9), atherogenic (n = 10), modified atherogenic (n = 6), or eucaloric standard diet (n = 12) for 24 weeks. Serum chemistries were measured, and pancreata were examined for histological abnormalities including steatosis, inflammation or fibrosis, insulin content, and oxidative stress., Results: The fructose, atherogenic, and modified atherogenic diet groups exhibited obesity, metabolic syndrome, islet enlargement, and significantly increased pancreatic steatosis (22.9% ± 7.5%, 19.7% ± 7.7%, and 38.7% ± 15.3% fat in total tissue area, respectively) compared with controls (9.3% ± 1.9%; P < 0.05). The modified atherogenic diet group showed significantly increased oxidative stress levels as evidenced by elevated serum malondialdehyde (3.0 ± 3.3 vs 1.5 ± 0.3 μmol/L in controls; P = 0.006) and pancreatic malondialdehyde (0.1 ± 0.12 vs 0.04 ± 0.01 nmol/mg protein in controls; P = 0.01). None of the swine exhibited pancreatitis or cellular injury., Conclusions: Ossabaw swine fed with a modified atherogenic diet developed significant pancreatic steatosis and increased oxidative stress, but no other histological abnormalities were observed.

The purpose of the present study was to determine whether ultrasound is a useful tool to measure the venous characteristics of the lower extremity during a standard venous collecting cuff deflation protocol. To accomplish this, lower extremity pressure-cross-sectional area (CSA) and pressure-volume relationships were measured in eight (25 +/- 1 yr) supine subjects. Popliteal vein CSA was assessed by using high-resolution ultrasound, while calf volume changes were simultaneously assessed by using venous occlusion plethysmography (VOP). Pressure-CSA and pressure-volume relationships were assessed at baseline, during the cold pressor (CP) test, and following sublingual nitroglycerin (NTG) administration. Relationships were modeled with a quadratic regression equation, and beta(1) and beta(2) were used as indexes of venous compliance. Popliteal vein regression parameters beta(1) (8.485 +/- 2.616 vs. 7.638 +/- 2.664, baseline vs. CP; 8.485 +/- 2.616 vs. 7.734 +/- 3.076, baseline vs. NTG; both P > 0.05) and beta(2) (-0.0841 +/- 0.0241 vs. -0.0793 +/- 0.0242, baseline vs. CP; -0.0841 +/- 0.0241 vs. -0.0771 +/- 0.0280, baseline vs. NTG; both P > 0.05) were not affected by CP or NTG. Similarly, calf regression parameters beta(1) and beta(2), obtained with VOP, were not altered during either trial. Intraclass correlations for venous compliance assessed with ultrasound and VOP were 0.92 and 0.97, respectively, indicating acceptable reproducibility. These data suggest that ultrasound is a functional and reproducible tool to assess the venous characteristics of the lower extremity, in addition to VOP. Additionally, popliteal vein and calf compliance were not affected by the CP test or NTG.

It is commonly assumed that the male hamsters' flank glands subserve an agonistic role with other males as the intended recipients of the olfactory message. Because of little evidence to support such a relationship, two studies investigated whether females might selectively respond to male flank odors. In a free choice situation females spent more time in proximity with glandular rubbings obtained from intact males than those obtained from castrates or a clean control. Moreover, these sexually receptive females remained longer near odors from familiar as opposed to novel males. The second experiment demonstrated that receptive females spent more time in proximity with flank odors of dominant males as opposed to their subordinate partners or to a clean control. Females can use flank gland odors to discriminate among males as a function of their hormonal state, familiarity and dominance. If this occurs in the wild, such odors could provide cues which would facilitate intersexual selection.

It is commonly assumed that sexually dimorphic cutaneous glands are important in supporting inter-male aggression in rodents. Such an intimidation role has been hypothesized as the function of the hamsters' flank gland. Two experiments failed to find avoidance of male flank odors. In one, subordinate males entered and spent comparable amounts of time in clean areas and areas scented with the dominant's flank secretion. In the other study, males were found to respond differentially to flank odors obtained from the subject, a familiar and a novel male. Although the males seemed to have habituated to the odor of the familiar male, there was no suggestion of avoidance of any of these odors. It may be that the males' flank odor is relevant not to other males but to females.