16 results on '"Fulga F"'
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2. Multi-threading protein surface functional description.
- Author
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Cristea, P.D., Tuduce, R., Arsene, O., Nicolau, D., and Fulga, F.
- Published
- 2010
- Full Text
- View/download PDF
3. Interrogation of the dynamics of magnetic microbeads on the meso-scale via electromagnetic detection
- Author
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Fulga, F, primary, Myhra, S, additional, and Nicolau, D V, additional
- Published
- 2002
- Full Text
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4. The Re-/Up-Cycling of Wood Waste in Wood-Polymer Composites (WPCs) for Common Applications.
- Author
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Teacă CA, Shahzad A, Duceac IA, and Tanasă F
- Abstract
Wood-polymer composites (WPCs) are a class of materials intensively studied and promoted in the context of sustainable development, mainly when aspects related to the increasing awareness of environmental issues and waste management are considered. Feasible opportunities for producing WPCs with value-added properties intended for common applications emerge when polymers, either synthetic or from renewable resources, raw or waste, are employed in re-/up-cycling approaches. In this context, some examples of easily achievable WPCs are presented herein, namely, formulations based on different wood waste and polymer matrices (synthetic: polypropylene and malleated polypropylene as a compatibilizer; natural: plasticized starch). Their level of performance was assessed through different characterization methods (FTIR, WAXD, TGA, DSC, mechanical test, etc.). The benefits and limitations of this approach are also discussed.
- Published
- 2023
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5. Insights on Some Polysaccharide Gel Type Materials and Their Structural Peculiarities.
- Author
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Duceac IA, Stanciu MC, Nechifor M, Tanasă F, and Teacă CA
- Abstract
Global resources have to be used in responsible ways to ensure the world's future need for advanced materials. Ecologically friendly functional materials based on biopolymers can be successfully obtained from renewable resources, and the most prominent example is cellulose, the well-known most abundant polysaccharide which is usually isolated from highly available biomass (wood and wooden waste, annual plants, cotton, etc.). Many other polysaccharides originating from various natural resources (plants, insects, algae, bacteria) proved to be valuable and versatile starting biopolymers for a wide array of materials with tunable properties, able to respond to different societal demands. Polysaccharides properties vary depending on various factors (origin, harvesting, storage and transportation, strategy of further modification), but they can be processed into materials with high added value, as in the case of gels. Modern approaches have been employed to prepare (e.g., the use of ionic liquids as "green solvents") and characterize (NMR and FTIR spectroscopy, X ray diffraction spectrometry, DSC, electronic and atomic force microscopy, optical rotation, circular dichroism, rheological investigations, computer modelling and optimization) polysaccharide gels. In the present paper, some of the most widely used polysaccharide gels will be briefly reviewed with emphasis on their structural peculiarities under various conditions.
- Published
- 2022
- Full Text
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6. Selective Oxidation of Cellulose-A Multitask Platform with Significant Environmental Impact.
- Author
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Duceac IA, Tanasa F, and Coseri S
- Abstract
Raw cellulose, or even agro-industrial waste, have been extensively used for environmental applications, namely industrial water decontamination, due to their effectiveness, availability, and low production cost. This was a response to the increasing societal demand for fresh water, which made the purification of wastewater one of the major research issue for both academic and industrial R&D communities. Cellulose has undergone various derivatization reactions in order to change the cellulose surface charge density, a prerequisite condition to delaminate fibers down to nanometric fibrils through a low-energy process, and to obtain products with various structures and properties able to undergo further processing. Selective oxidation of cellulose, one of the most important methods of chemical modification, turned out to be a multitask platform to obtain new high-performance, versatile, cellulose-based materials, with many other applications aside from the environmental ones: in biomedical engineering and healthcare, energy storage, barrier and sensing applications, food packaging, etc. Various methods of selective oxidation have been studied, but among these, (2,2,6,6-tetramethylpiperidin-1-yl)oxyl) (TEMPO)-mediated and periodate oxidation reactions have attracted more interest due to their enhanced regioselectivity, high yield and degree of substitution, mild conditions, and the possibility to further process the selectively oxidized cellulose into new materials with more complex formulations. This study systematically presents the main methods commonly used for the selective oxidation of cellulose and provides a survey of the most recent reports on the environmental applications of oxidized cellulose, such as the removal of heavy metals, dyes, and other organic pollutants from the wastewater.
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- 2022
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7. Valorization of lignin in polymer and composite systems for advanced engineering applications - A review.
- Author
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Collins MN, Nechifor M, Tanasă F, Zănoagă M, McLoughlin A, Stróżyk MA, Culebras M, and Teacă CA
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- Biopolymers, Chemical Fractionation, Lignin isolation & purification, Bioengineering, Lignin chemistry, Polymers chemistry
- Abstract
As fossil fuel resources dwindle and new regulations for a cleaner and safer environment come on stream, there is growing interest in developing new sustainable feedstocks for future fuels, chemicals, polymers and fibers. Therefore materials research is ever more focused on the production of green or bio-based materials and their composites. Lignocellulosic biomass has become the feedstock of choice for these new materials as cellulose and lignin are the most abundant biopolymers on the planet. Lignin is a phenolic macromolecule, the principal biological source of aromatic structures, with a complex structure which varies depending on plant species and its isolation process. Despite its high carbon content and its potential as a raw material, lignin remains underutilised. Between 40 and 50 million tons of lignin are produced worldwide per year; while some is being used for low- and medium-value applications, most is currently treated as a non-commercialized by-product or as low value fuel to produce energy. However, with the emergence of biorefinery projects larger amounts of lignin with the potential for valorisation are being produced. Here, we summarise some of the latest developments in the field., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
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8. Evaluation of Vascular Proliferation in Molecular Subtypes of Breast Cancer.
- Author
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Bujor IS, Cioca A, Ceaușu RA, Veaceslav F, Nica C, Cîmpean AM, and Raica M
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- Adult, Aged, Aged, 80 and over, Antigens, CD34 metabolism, Breast Neoplasms blood supply, Breast Neoplasms classification, Female, Humans, Immunohistochemistry, Ki-67 Antigen metabolism, Microvessels pathology, Middle Aged, Prognosis, Breast Neoplasms metabolism, Cell Proliferation, Microvessels metabolism, Neovascularization, Pathologic metabolism
- Abstract
Background: Angiogenesis plays a pivotal role in tumor development. Although microvessel density (MVD) is the most common method used for evaluation of angiogenesis, it has several limitations. Our aim was to evaluate MVD and microvessel proliferation (MVP) in a series of invasive breast carcinomas and analyze whether angiogenesis is influenced by the molecular phenotype of each tumor., Materials and Methods: We examined vascular proliferation using double immunohistochemistry (CD34/Ki67) in a series of 54 invasive breast carcinomas and compared the results with standard MVD, molecular subtypes and other classical parameters., Results: Increased MVD and MVP values were recorded in basal-like subtype, but only the MVP value reached significance among this group of patients (p=0.0001). For all cases combined, increased MVP was significantly correlated with negative estrogen receptor (ER) status (p=0.010) and higher histological grade (p=0.002)., Conclusion: MVP more accurately reflects the state of angiogenesis in breast cancer, compared with standard MVD. Vascular proliferation was associated with aggressive tumor features, indicating its contribution to tumor progression. The strong association between vascular proliferation and basal-like tumors suggests that this marker can be used for stratification of patients who might benefit from therapies targeting angiogenesis., (Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2018
- Full Text
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9. Polymer surface properties control the function of heavy meromyosin in dynamic nanodevices.
- Author
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Hanson KL, Fulga F, Dobroiu S, Solana G, Kaspar O, Tokarova V, and Nicolau DV
- Subjects
- Actin Cytoskeleton chemistry, Actin Cytoskeleton physiology, Myosin Subfragments physiology, Myosins chemistry, Myosins physiology, Polymers chemistry, Quartz Crystal Microbalance Techniques, Surface Properties, Biosensing Techniques, Myosin Subfragments chemistry, Nanotechnology
- Abstract
The actin-myosin system, responsible for muscle contraction, is also the force-generating element in dynamic nanodevices operating with surface-immobilized motor proteins. These devices require materials that are amenable to micro- and nano-fabrication, but also preserve the bioactivity of molecular motors. The complexity of the protein-surface systems is greatly amplified by those of the polymer-fluid interface; and of the structure and function of molecular motors, making the study of these interactions critical to the success of molecular motor-based nanodevices. We measured the density of the adsorbed motor protein (heavy meromyosin, HMM) using quartz crystal microbalance; and motor bioactivity with ATPase assay, on a set of model surfaces, i.e., nitrocellulose, polystyrene, poly(methyl methacrylate), and poly(butyl methacrylate), poly(tert-butyl methacrylate). A higher hydrophobicity of the adsorbing material translates in a higher total number of HMM molecules per unit area, but also in a lower uptake of water, and a lower ratio of active per total HMM molecules per unit area. We also measured the motility characteristics of actin filaments on the model surfaces, i.e., velocity, smoothness and deflection of movement, determined via in vitro motility assays. The filament velocities were found to be controlled by the relative number of active HMM per total motors, rather than their absolute surface density. The study allowed the formulation of the general engineering principles for the selection of polymeric materials for the manufacturing of dynamic nanodevices using protein molecular motors., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2017
- Full Text
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10. Mapping hydrophobicity on the protein molecular surface at atom-level resolution.
- Author
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Nicolau DV Jr, Paszek E, Fulga F, and Nicolau DV
- Subjects
- Protein Structure, Tertiary, Surface Properties, Hydrophobic and Hydrophilic Interactions, Models, Molecular, Proteins chemistry
- Abstract
A precise representation of the spatial distribution of hydrophobicity, hydrophilicity and charges on the molecular surface of proteins is critical for the understanding of the interaction with small molecules and larger systems. The representation of hydrophobicity is rarely done at atom-level, as this property is generally assigned to residues. A new methodology for the derivation of atomic hydrophobicity from any amino acid-based hydrophobicity scale was used to derive 8 sets of atomic hydrophobicities, one of which was used to generate the molecular surfaces for 35 proteins with convex structures, 5 of which, i.e., lysozyme, ribonuclease, hemoglobin, albumin and IgG, have been analyzed in more detail. Sets of the molecular surfaces of the model proteins have been constructed using spherical probes with increasingly large radii, from 1.4 to 20 Å, followed by the quantification of (i) the surface hydrophobicity; (ii) their respective molecular surface areas, i.e., total, hydrophilic and hydrophobic area; and (iii) their relative densities, i.e., divided by the total molecular area; or specific densities, i.e., divided by property-specific area. Compared with the amino acid-based formalism, the atom-level description reveals molecular surfaces which (i) present an approximately two times more hydrophilic areas; with (ii) less extended, but between 2 to 5 times more intense hydrophilic patches; and (iii) 3 to 20 times more extended hydrophobic areas. The hydrophobic areas are also approximately 2 times more hydrophobicity-intense. This, more pronounced "leopard skin"-like, design of the protein molecular surface has been confirmed by comparing the results for a restricted set of homologous proteins, i.e., hemoglobins diverging by only one residue (Trp37). These results suggest that the representation of hydrophobicity on the protein molecular surfaces at atom-level resolution, coupled with the probing of the molecular surface at different geometric resolutions, can capture processes that are otherwise obscured to the amino acid-based formalism.
- Published
- 2014
- Full Text
- View/download PDF
11. Protein molecular surface mapped at different geometrical resolutions.
- Author
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Nicolau DV, Paszek E, Fulga F, and Nicolau DV Jr
- Subjects
- Adsorption, Animals, Electrons, Humans, Hydrophobic and Hydrophilic Interactions, Linear Models, Nanoparticles, Point Mutation, Protein Conformation, Proteins genetics, Surface Properties, Models, Molecular, Proteins chemistry, Proteins metabolism
- Abstract
Many areas of biochemistry and molecular biology, both fundamental and applications-orientated, require an accurate construction, representation and understanding of the protein molecular surface and its interaction with other, usually small, molecules. There are however many situations when the protein molecular surface gets in physical contact with larger objects, either biological, such as membranes, or artificial, such as nanoparticles. The contribution presents a methodology for describing and quantifying the molecular properties of proteins, by geometrical and physico-chemical mapping of the molecular surfaces, with several analytical relationships being proposed for molecular surface properties. The relevance of the molecular surface-derived properties has been demonstrated through the calculation of the statistical strength of the prediction of protein adsorption. It is expected that the extension of this methodology to other phenomena involving proteins near solid surfaces, in particular the protein interaction with nanoparticles, will result in important benefits in the understanding and design of protein-specific solid surfaces.
- Published
- 2013
- Full Text
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12. Protein immobilisation on micro/nanostructures fabricated by laser microablation.
- Author
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Nicolau DV, Ivanova EP, Fulga F, Filipponi L, Viezzoli A, Dobroiu S, Alekseeva YV, and Pham DK
- Subjects
- Adsorption, Chymotrypsin, Humans, Immunoglobulin G, Lasers, Microscopy, Atomic Force, Muramidase, Myoglobin, Protein Array Analysis instrumentation, Serum Albumin, Surface Properties, Temperature, Immobilized Proteins, Nanostructures, Protein Array Analysis methods
- Abstract
The performance of biomedical microdevices requires the accurate control of the biomolecule concentration on the surface, as well as the preservation of their bioactivity. This desideratum is even more critical for proteins, which present a significant propensity for surface-induced denaturation, and for microarrays, which require high multiplexing. We have previously proposed a method for protein immobilisation on micro/nanostructures fabricated via laser ablation of a thin metal layer deposited on a transparent polymer. This study investigates the relationship between the properties of the micro/nanostructured surface, i.e., topography and physico-chemistry, and protein immobilisation, for five, molecularly different proteins, i.e., lysozyme, myoglobin, α-chymotrypsin, human serum albumin, and human immunoglobulin. Protein immobilisation on microstructures has been characterised using quantitative fluorescence measurements and atomic force microscopy. It has been found that the sub-micrometer-level, combinatorial nature of the microstructure translates in a 3-10-fold amplification of protein adsorption, as compared to flat, chemically homogenous polymeric surfaces. This amplification is more pronounced for smaller proteins, as they can capitalize better on the newly created surface and variability of the nano-environments., (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
- Full Text
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13. Microbeads on microposts: an inverted architecture for bead microarrays.
- Author
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Filipponi L, Sawant PD, Fulga F, and Nicolau DV
- Subjects
- Equipment Design, Equipment Failure Analysis, Microspheres, Reproducibility of Results, Sensitivity and Specificity, Biosensing Techniques instrumentation, Dimethylpolysiloxanes chemistry, Protein Array Analysis instrumentation
- Abstract
The rapid development of genomics and proteomics requires accelerated improvement of the microarrays density, multiplexing, readout capabilities and cost-effectiveness. The bead arrays are increasingly attractive because of their self-assembly-based fabrication, which alleviates many problems of top-down microfabrication. Here we present a simple, reliable, robust and modular technique for the fabrication of bead microarrays, which combines the directed assembling of beads in microstructures and PDMS-based replica molding. The beads are first self-assembled in pyramidal microwells fabricated by anisotropic etching of silicon substrates, then transferred on the apex of PDMS pyramids that replicate the silicon microstructures. The arrays are chemically and biochemically robust; they are spatially addressable and have the potential for being informationally addressable; and they appear to offer better readout capabilities than the classical microarrays.
- Published
- 2009
- Full Text
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14. Models of protein linear molecular motors for dynamic nanodevices.
- Author
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Fulga F, Nicolau DV Jr, and Nicolau DV
- Subjects
- Models, Molecular, Molecular Dynamics Simulation, Adenosine Triphosphate physiology, Molecular Motor Proteins physiology, Nanotechnology methods
- Abstract
Protein molecular motors are natural nano-machines that convert the chemical energy from the hydrolysis of adenosine triphosphate into mechanical work. These efficient machines are central to many biological processes, including cellular motion, muscle contraction and cell division. The remarkable energetic efficiency of the protein molecular motors coupled with their nano-scale has prompted an increasing number of studies focusing on their integration in hybrid micro- and nanodevices, in particular using linear molecular motors. The translation of these tentative devices into technologically and economically feasible ones requires an engineering, design-orientated approach based on a structured formalism, preferably mathematical. This contribution reviews the present state of the art in the modelling of protein linear molecular motors, as relevant to the future design-orientated development of hybrid dynamic nanodevices.
- Published
- 2009
- Full Text
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15. Surface hydrophobicity modulates the operation of actomyosin-based dynamic nanodevices.
- Author
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Nicolau DV, Solana G, Kekic M, Fulga F, Mahanivong C, Wright J, Ivanova EP, and dos Remedios CG
- Subjects
- Adsorption, Polymers chemistry, Protein Denaturation, Proteins chemistry, Surface Properties, Actomyosin chemistry, Nanotechnology
- Abstract
We studied the impact of surface hydrophobicity on the motility of actin filaments moving on heavy-meromyosin (HMM)-coated surfaces. Apart from nitrocellulose (NC), which is the current standard for motility assays, all materials tested are good candidates for microfabrication: hydrophilic and hydrophobic glass, poly(methyl methacrylate) (PMMA), poly(tert-butyl methacrylate) (PtBuMA), and a copolymer of O-acryloyl acetophenone oxime with a 4-acryloyloxybenzophenone (AAPO). The most hydrophilic (hydrophilic glass, contact angle 35 degrees) and the most hydrophobic (PtBuMA, contact angle 78 degrees) surfaces do not maintain the motility of actin filaments, presumably because of the low density of adsorbed HMM protein or its high levels of denaturation, respectively. The velocity of actin filaments presents higher values in the middle of this "surface hydrophobicity motility window" (NC, PMMA), and a bimodal distribution, which is more apparent at the edges of this motility window (hydrophobic glass and AAPO). A molecular surface analysis of HMM and its S1 units suggests that the two very different, temporally separated conformations of the HMM heads could exacerbate the surface-modulated protein behavior, which is common to all microdevices using surface-immobilized proteins. An explanation for the above behavior proposes that the motility of actin filaments on HMM-functionalized surfaces is the result of the action of three populations of motors, each in a different surface-protein conformation, that is, HMM with both heads working (high velocities), working with one head (low velocities), and fully denatured HMM (no motility). It is also proposed that the molecularly dynamic nature of polymer surfaces amplifies the impact of surface hydrophobicity on protein behavior. The study demonstrates that PMMA is a good candidate for the fabrication of future actomyosin-driven dynamic nanodevices because it induces the smoothest motility of individual nano-objects with velocities comparable with those obtained on NC.
- Published
- 2007
- Full Text
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16. A novel approach in cinnamic acid synthesis: direct synthesis of cinnamic acids from aromatic aldehydes and aliphatic carboxylic acids in the presence of boron tribromide.
- Author
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Chiriac CI, Tanasa F, and Onciu M
- Subjects
- Boron Compounds chemistry, Bromides chemistry, Fatty Acids chemistry, Heterocyclic Compounds chemistry, Models, Biological, Aldehydes chemistry, Boron Compounds pharmacology, Bromides pharmacology, Carboxylic Acids chemistry, Chemistry, Organic methods, Cinnamates chemical synthesis
- Abstract
Cinnamic acids have been prepared in moderate to high yields by a new direct synthesis using aromatic aldehydes and aliphatic carboxylic acids, in the presence of boron tribromide as reagent, 4-dimethylaminopyridine (4-DMAP) and pyridine (Py) as bases and N-methyl-2-pyrolidinone (NMP) as solvent, at reflux (180-190 degrees C) for 8-12 hours.
- Published
- 2005
- Full Text
- View/download PDF
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