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2. Pharmacological Evidence That Dictyostelium Differentiation-Inducing Factor 1 Promotes Glucose Uptake Partly via an Increase in Intracellular cAMP Content in Mouse 3T3-L1 Cells.

Author

Kubohara, Yuzuru, Fukunaga, Yuko, Kikuchi, Haruhisa, and Kuwayama, Hidekazu

Subjects
*DICTYOSTELIUM, *GLUCOSE, *DICTYOSTELIUM discoideum, *ADENYLATE cyclase, *MYXOMYCETES, *INSULIN receptors, *FORSKOLIN
Abstract

Differentiation-inducing factor 1 (DIF-1) isolated from the cellular slime mold Dictyostelium discoideum can inhibit mammalian calmodulin-dependent cAMP/cGMP phosphodiesterase (PDE1) in vitro. DIF-1 also promotes glucose uptake, at least in part, via a mitochondria- and AMPK-dependent pathway in mouse 3T3-L1 fibroblast cells, but the mechanism underlying this effect has not been fully elucidated. In this study, we investigated the effects of DIF-1 on intracellular cAMP and cGMP levels, as well as the effects that DIF-1 and several compounds that increase cAMP and cGMP levels have on glucose uptake in confluent 3T3-L1 cells. DIF-1 at 20 μM (a concentration that promotes glucose uptake) increased the level of intracellular cAMP by about 20% but did not affect the level of intracellular cGMP. Neither the PDE1 inhibitor 8-methoxymethyl-3-isobutyl-1-methylxanthine at 10–200 μM nor the broad-range PDE inhibitor 3-isobutyl-1-methylxanthine at 40–400 μM had any marked effects on glucose uptake. The membrane-permeable cAMP analog 8-bromo-cAMP at 200–1000 μM significantly promoted glucose uptake (by 20–25%), whereas the membrane-permeable cGMP analog 8-bromo-cGMP at 3–100 μM did not affect glucose uptake. The adenylate cyclase activator forskolin at 1–10 μM promoted glucose uptake by 20–30%. Thus, DIF-1 may promote glucose uptake by 3T3-L1 cells, at least in part, via an increase in intracellular cAMP level. [ABSTRACT FROM AUTHOR]

Published
2023
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14. Structural and Functional Basis for LILRB Immune Checkpoint Receptor Recognition of HLA-G Isoforms

Author

Kuroki, Kimiko, primary, Matsubara, Haruki, additional, Kanda, Ryo, additional, Miyashita, Naoyuki, additional, Shiroishi, Mitsunori, additional, Fukunaga, Yuko, additional, Kamishikiryo, Jun, additional, Fukunaga, Atsushi, additional, Fukuhara, Hideo, additional, Hirose, Kaoru, additional, Hunt, Joan S., additional, Sugita, Yuji, additional, Kita, Shunsuke, additional, Ose, Toyoyuki, additional, and Maenaka, Katsumi, additional

Published
2019
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17. Publisher Correction: Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport

Author

Toba, Shiori, primary, Jin, Mingyue, additional, Yamada, Masami, additional, Kumamoto, Kanako, additional, Matsumoto, Sakiko, additional, Yasunaga, Takuo, additional, Fukunaga, Yuko, additional, Miyazawa, Atsuo, additional, Fujita, Sakiko, additional, Itoh, Kyoko, additional, Fushiki, Shinji, additional, Kojima, Hiroaki, additional, Wanibuchi, Hideki, additional, Arai, Yoshiyuki, additional, Nagai, Takeharu, additional, and Hirotsune, Shinji, additional

Published
2018
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19. Ideal Osmotic Spaces for Chlorobionts or Cyanobionts Are Differentially Realized by Lichenized Fungi1[W][OPEN]

Author

Kosugi, Makiko, Shizuma, Ryoko, Moriyama, Yufu, Koike, Hiroyuki, Fukunaga, Yuko, Takeuchi, Akihisa, Uesugi, Kentaro, Suzuki, Yoshio, Imura, Satoshi, Kudoh, Sakae, Miyazawa, Atsuo, Kashino, Yasuhiro, and Satoh, Kazuhiko

Subjects
Nostoc commune, Lichens, Chlorophyta, Osmotic Pressure, Water, Articles, Photosynthesis, Symbiosis
Abstract

Lichens result from symbioses between a fungus and either a green alga or a cyanobacterium. They are known to exhibit extreme desiccation tolerance. We investigated the mechanism that makes photobionts biologically active under severe desiccation using green algal lichens (chlorolichens), cyanobacterial lichens (cyanolichens), a cephalodia-possessing lichen composed of green algal and cyanobacterial parts within the same thallus, a green algal photobiont, an aerial green alga, and a terrestrial cyanobacterium. The photosynthetic response to dehydration by the cyanolichen was almost the same as that of the terrestrial cyanobacterium but was more sensitive than that of the chlorolichen or the chlorobiont. Different responses to dehydration were closely related to cellular osmolarity; osmolarity was comparable between the cyanolichen and a cyanobacterium as well as between a chlorolichen and a green alga. In the cephalodium-possessing lichen, osmolarity and the effect of dehydration on cephalodia were similar to those exhibited by cyanolichens. The green algal part response was similar to those exhibited by chlorolichens. Through the analysis of cellular osmolarity, it was clearly shown that photobionts retain their original properties as free-living organisms even after lichenization.

Published
2014

23. Ideal Osmotic Spaces for Chlorobionts or Cyanobionts Are Differentially Realized by Lichenized Fungi

Author

Kosugi, Makiko, primary, Shizuma, Ryoko, additional, Moriyama, Yufu, additional, Koike, Hiroyuki, additional, Fukunaga, Yuko, additional, Takeuchi, Akihisa, additional, Uesugi, Kentaro, additional, Suzuki, Yoshio, additional, Imura, Satoshi, additional, Kudoh, Sakae, additional, Miyazawa, Atsuo, additional, Kashino, Yasuhiro, additional, and Satoh, Kazuhiko, additional

Published
2014
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25. The long-term immunosuppressive effects of disulfide-linked HLA-G dimer in mice with collagen-induced arthritis

Author

Kuroki, Kimiko, Hirose, Kaoru, Okabe, Yuki, Fukunaga, Yuko, Takahashi, Ami, Shiroishi, Mitsunori, Kajikawa, Mizuho, Tabata, Shigekazu, Nakamura, Seiko, Takai, Toshiyuki, Koyanagi, Satoru, Ohdo, Shigehiro, Maenaka, Katsumi, Kuroki, Kimiko, Hirose, Kaoru, Okabe, Yuki, Fukunaga, Yuko, Takahashi, Ami, Shiroishi, Mitsunori, Kajikawa, Mizuho, Tabata, Shigekazu, Nakamura, Seiko, Takai, Toshiyuki, Koyanagi, Satoru, Ohdo, Shigehiro, and Maenaka, Katsumi

Abstract

HLA-G, a natural immunosuppressant present in the human placenta during pregnancy, prevents fetal destruction by the maternal immune system. The immunosuppressive effect of HLA-G is mediated by the immune cell inhibitory receptors, LILRB1 and LILRB2. HLA-G forms disulfide-linked dimers by natural oxidation, and the dimer associates with LILRB1/B2 much more strongly than the monomer. Furthermore, the dimer formation remarkably enhanced the LILRB-mediated signaling. In this report, we studied the in vivo immunosuppressive effect of the HLA-G dimer, using the collagen-induced arthritis model mouse. Mice were treated with the HLA-G monomer or dimer intracutaneously at the left foot joint, once or for 5 days, and the clinical severity was evaluated daily in a double-blind study. The HLA-G monomer and dimer both produced excellent anti-inflammatory effects with a single, local administration. Notably, as compared to the monomer, the dimer exhibited significant immunosuppressive effects at lower concentrations, which persisted for about two months. In accordance with this result, a binding study revealed that the HLA-G dimer binds PIR-B, the mouse homolog of the LILRBs, with higher affinity and avidity than the monomer. The HLA-G dimer is expected to be quite useful as an anti-rheumatoid arthritis agent, in small amounts with minimal side effects. (C) 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

Published
2013

26. The long-term immunosuppressive effects of disulfide-linked HLA-G dimer in mice with collagen-induced arthritis

Author

1000090553313, Kuroki, Kimiko, Hirose, Kaoru, Okabe, Yuki, Fukunaga, Yuko, Takahashi, Ami, Shiroishi, Mitsunori, Kajikawa, Mizuho, Tabata, Shigekazu, Nakamura, Seiko, 1000020187917, Takai, Toshiyuki, 1000060330932, Koyanagi, Satoru, 1000000223884, Ohdo, Shigehiro, 1000010322752, Maenaka, Katsumi, 1000090553313, Kuroki, Kimiko, Hirose, Kaoru, Okabe, Yuki, Fukunaga, Yuko, Takahashi, Ami, Shiroishi, Mitsunori, Kajikawa, Mizuho, Tabata, Shigekazu, Nakamura, Seiko, 1000020187917, Takai, Toshiyuki, 1000060330932, Koyanagi, Satoru, 1000000223884, Ohdo, Shigehiro, 1000010322752, and Maenaka, Katsumi

Abstract

HLA-G, a natural immunosuppressant present in the human placenta during pregnancy, prevents fetal destruction by the maternal immune system. The immunosuppressive effect of HLA-G is mediated by the immune cell inhibitory receptors, LILRB1 and LILRB2. HLA-G forms disulfide-linked dimers by natural oxidation, and the dimer associates with LILRB1/B2 much more strongly than the monomer. Furthermore, the dimer formation remarkably enhanced the LILRB-mediated signaling. In this report, we studied the in vivo immunosuppressive effect of the HLA-G dimer, using the collagen-induced arthritis model mouse. Mice were treated with the HLA-G monomer or dimer intracutaneously at the left foot joint, once or for 5 days, and the clinical severity was evaluated daily in a double-blind study. The HLA-G monomer and dimer both produced excellent anti-inflammatory effects with a single, local administration. Notably, as compared to the monomer, the dimer exhibited significant immunosuppressive effects at lower concentrations, which persisted for about two months. In accordance with this result, a binding study revealed that the HLA-G dimer binds PIR-B, the mouse homolog of the LILRBs, with higher affinity and avidity than the monomer. The HLA-G dimer is expected to be quite useful as an anti-rheumatoid arthritis agent, in small amounts with minimal side effects. (C) 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

Published
2013

27. The long-term immunosuppressive effects of disulfide-linked HLA-G dimer in mice with collagen-induced arthritis

Author

Kuroki, Kimiko, primary, Hirose, Kaoru, additional, Okabe, Yuki, additional, Fukunaga, Yuko, additional, Takahashi, Ami, additional, Shiroishi, Mitsunori, additional, Kajikawa, Mizuho, additional, Tabata, Shigekazu, additional, Nakamura, Seiko, additional, Takai, Toshiyuki, additional, Koyanagi, Satoru, additional, Ohdo, Shigehiro, additional, and Maenaka, Katsumi, additional

Published
2013
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29. Distinct HIV-1 Escape Patterns Selected by Cytotoxic T Cells with Identical Epitope Specificity

Author

Yagita, Yuichi, primary, Kuse, Nozomi, additional, Kuroki, Kimiko, additional, Gatanaga, Hiroyuki, additional, Carlson, Jonathan M., additional, Chikata, Takayuki, additional, Brumme, Zabrina L., additional, Murakoshi, Hayato, additional, Akahoshi, Tomohiro, additional, Pfeifer, Nico, additional, Mallal, Simon, additional, John, Mina, additional, Ose, Toyoyuki, additional, Matsubara, Haruki, additional, Kanda, Ryo, additional, Fukunaga, Yuko, additional, Honda, Kazutaka, additional, Kawashima, Yuka, additional, Ariumi, Yasuo, additional, Oka, Shinichi, additional, Maenaka, Katsumi, additional, and Takiguchi, Masafumi, additional

Published
2013
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31. PINK1 autophosphorylation upon membrane potential dissipation is essential for Parkin recruitment to damaged mitochondria

Author

Okatsu, Kei, primary, Oka, Toshihiko, additional, Iguchi, Masahiro, additional, Imamura, Kenji, additional, Kosako, Hidetaka, additional, Tani, Naoki, additional, Kimura, Mayumi, additional, Go, Etsu, additional, Koyano, Fumika, additional, Funayama, Manabu, additional, Shiba-Fukushima, Kahori, additional, Sato, Shigeto, additional, Shimizu, Hideaki, additional, Fukunaga, Yuko, additional, Taniguchi, Hisaaki, additional, Komatsu, Masaaki, additional, Hattori, Nobutaka, additional, Mihara, Katsuyoshi, additional, Tanaka, Keiji, additional, and Matsuda, Noriyuki, additional

Published
2012
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