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The methanol extract of Yucca schidigera (YE) showed a suppressive effect on umu gene expression of the SOS response induced by 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) in Salmonella typhimurium TA1535/pSK1002. The suppressive effect of YE was also observed for 2-aminoanthracene and activated Trp-P-1, without a significant effect on bacterial growth. The extract exhibited a weak suppressive effect on SOS-induction by N-methyl-N'-nitro-N-nitrosoguanidine, but not by furylfuramide or 4-nitroquinoline-1-oxide. The antimutagenic activity of YE against Trp-P-1 was demonstrated by Ames assay using Salmonella typhimurium TA98. Isolation and purification of the active component of YE was carried out using SiO2 column chromatography, and 275 mg of antimutagenic compound was isolated from 2.5 kg of dried chips of yucca roots and branches. The compound was identified as 3,4',5-trihydroxystilbene (THS). The SOS suppression and antimutagenicity of THS against Trp-P-1 was determined by umu test and Ames test.

An adequate amount of typhoid-paratyphoid vaccine (TPV) or bovine liver lipid which had been emulsified in bovine serum was injected intravenously to rabbits of two groups two times at an interval of 4 days. After the second sensitization, it was found that a new specific substance had appeared in the circulating blood of each rabbit, and that it had accelerated the endogenous respiration of mouse liver cells remarkably under aerobic conditions.An effective serum was used as a starting material and examined first for stability against heat, acid, and alkali and dialyzability agains semi-dialytic cellophane membrane. Then, the extraction and purification were carried out by the column-chromatographic method. In this method, the deproteinized serum filtrate was absorbed on Amberlite IR 120H+ resin with 100-200 meshes. After that, the effective substance was extracted as a single substance through the column-chromatographic fractionation by effusion of 0.1N-HCl solution. Finally, it was identified as uric acid by paper chromatography, continuous ultraviolet and infrared absorption spectra, element dialysis, and measurement of melting point. On the other hand, uric acid was compared with some metabolic intermediates of ATP, with the exception of uric acid itself, as substrates for the oxidative respiration of mouse liver homogenate or mitochondria.