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N6-methyladenosine (m6A) is the most abundant internal messenger RNA modification in eukaryotes, influencing various physiological and pathological processes by regulating RNA metabolism. Numerous studies have investigated the role of m6A in inflammatory responses and inflammatory diseases. In this study, VOSviewer and Citespace were used to perform bibliometric analysis to systematically evaluating the current landscape of research on the association between m6A and inflammation. The literature was sourced from the Web of Science Core Collection, with characteristics including year, country/region, institution, author, journal, citation, and keywords. According to the bibliometric analysis results of keywords, we present a narrative summary of the potential mechanisms by which m6A regulates inflammation. The results showed that the key mechanisms by which m6A modulates inflammation include apoptosis, autophagy, oxidative stress, immune cell dysfunction, and dysregulation of signaling pathways.

Abstract Background This study aimed to assess the methodological quality of the systematic reviews/meta-analyses (SRs/MAs) of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) using A Measurement Tool to Assess systematic Reviews (AMSTAR2) and to explore the potential influencing factors. Methods PubMed, EMBASE and Cochrane Library databases were searched for relevant studies. AMSTAR2 was used for evaluating the methodological quality of eligible SRs/MAs. Differences between methodological characteristics of SRs/MAs were compared using chi-square tests. The intra-class correlation coefficient (ICC) was used to assess reviewer agreement in the pre-experiment. Multivariate regression analysis was used to identify potential factors affecting methodological quality. Results A total of 45 SRs/MAs were included. After AMSTAR2 evaluation, only two (4.4%) of 45 SRs/MAs were moderate, three (6.7%) were rated as low quality, and the remainder 40 (88.9%) were rated as critically low quality. Among the 16 items of AMSTAR2, item 3 and item 10 had the poorest adherence. Item 4 received the most significant number of "Partial Yes" responses. Univariable analysis indicated that there were significant differences in methodological quality in SRs between different continents (P = 0.027) as well as between preregistered SRs and those that were not (P = 0.004). However, in multivariate analysis, there was no significant association between methodological quality and the following research characteristics: publication year, continent, whether reporting followed Preferred Reporting Items for Systematic Reviews (PRISMA), preregistration, funding support, randomized controlled trials (RCT) enrollment, whether SR was published in the Cochrane Database of Systematic Reviews (CDSR), and whether with meta-analysis. Additionally, subgroup analysis based on interventional SRs/MAs showed that continent was independently associated with the methodological quality of SRs/MAs of CP/CPPS via univariable and multivariate analysis. Conclusions Our study demonstrates that the methodological quality of SRs/MAs of CP/CPPS was generally poor. SRs/MAs of CP/CPPS should adopt the AMSTAR2 to enhance their methodological quality.

Colletotrichum gloeosporioides is the main pathogen that causes poplar anthracnose. This hemibiotrophic fungus, which can severely decrease the economic benefits and ecological functions of poplar trees, infects the host by forming an appressorium. Hox7 is an important regulatory factor that functions downstream of the Pmk1 MAPK signaling pathway. In this study, we investigated the effect of deleting CgHox7 on C. gloeosporioides. The conidia of the ΔCgHox7 deletion mutant germinated on a GelBond membrane to form non-melanized hyphal structures, but were unable to form appressoria. The deletion of CgHox7 weakened the ability of hyphae to penetrate a cellophane membrane and resulted in decreased virulence on poplar leaves. Furthermore, deleting CgHox7 affected the oxidative stress response. In the initial stage of appressorium formation, the accumulation of reactive oxygen species differed between the ΔCgHox7 deletion mutant and the wild-type control. Moreover, CgHox7 expression was necessary for maintaining cell wall integrity. Considered together, these results indicate that CgHox7 is a transcription factor with crucial regulatory effects on appressorium formation and the pathogenicity of C. gloeosporioides.

As one of the most densely populated, economically developed, and outwardly open urban agglomerations in China, the Pearl River Delta (PRD) urban agglomeration is a key player in achieving China’s carbon peak and carbon neutrality targets. This study analyzes low-emission development by examining the evolutionary patterns of carbon dioxide (CO2) emissions and the decoupling relationship between economic growth and CO2 emissions, using the latest available data from 2000 to 2020. Here are the main findings: (1) We found a significant fluctuation in the decoupling statuses between economic advancements and CO2 emissions within the PRD domain. Predominantly, a weak decoupling scenario was observed, where economic proliferations were paralleled by nearly equivalent increments in CO2 emissions. (2) The growth rate of carbon emissions increased significantly relative to economic expansion during 2015–2020, especially pronounced in cities such as Guangdong, Zhuhai, Foshan, and Dongguan. This delineates the persistent challenges in steering towards a pathway of energy conservation and emission abatement in the region. (3) Furthermore, a differential role of elasticity factors was noted across cities: Guangzhou and Shenzhen witnessed a significant influence of energy-saving elasticity in fostering a decoupling between economic surge and CO2 emissions, whereas in other cities, the emphasis shifted towards emission-reduction elasticity as a more vital determinant. The results of this study are of great significance for guiding policy makers and stakeholders in urban clusters across China and in similar regions globally to achieve low carbon development goals.

Against the backdrop of accelerating population aging and scarce elderly care resources and facilities in China, the issue of the sense of safety among the elderly has received widespread attention. This article is based on data from the China Labor Force Dynamics Survey (CLDS) in 2016 and takes three major urban agglomerations as examples to examine the impact mechanisms of urban built environments on elderly people’s sense of safety. The results indicate that the characteristics of the urban built environment, the social environment, and individual health affect the safety perceptions of the elderly. Among them, urbanization rate, hospital facilities, population density, greening rate, air quality, and frequency of dining out have significant impacts on elderly people’s sense of safety. Simultaneously, good daily exercise and mental health status can significantly improve elderly people’s sense of safety. This article summarizes the existing problems of aging-friendly spaces and facilities in three major urban agglomerations, proposes planning strategies to enhance elderly people’s sense of safety, and provides a useful reference for urban aging-friendly transformations and an elderly-friendly society.

Colletotrichum gloeosporioides is the causal agent of poplar anthracnose, which induces major economic losses and adversely affects the ecosystem services of poplar forests. The appressorium serves as a penetration structure for many pathogenic fungi, including C. gloeosporioides. The production of mucilage and the formation of penetration pegs are critically important for the appressorium-mediated penetration of host tissues. We previously found that CgPmk1 is a key protein involved in appressorium formation, penetration, and pathogenicity. Although CgSte12, which is a transcription factor that functions downstream of CgPmk1, regulates the formation of penetration pegs, its role in C. gloeosporioides appressorium development and pathogenicity has not been elucidated. Here, we developed C. gloeosporioides CgSTE12 mutants and characterized the molecular and cellular functions of CgSTE12. The results showed that mycelial growth and morphology were not affected in the CgSTE12 knockout mutants, which produced normal melanized appressoria. However, these mutants had less mucilage secreted around the appressoria, impaired appressorial cone formation, and the inability to form penetration pores and pegs, which ultimately led to a significant loss of pathogenicity. Our comparative transcriptome analysis revealed that CgSte12 controls the expression of genes involved in appressorium development and function, including genes encoding cutinases, NADPH oxidase, spermine biosynthesis-related proteins, ceramide biosynthesis-related proteins, fatty acid metabolism-related proteins, and glycerophospholipid metabolism-related proteins. Overall, our findings indicate that CgSte12 is a critical regulator of appressorium development and affects C. gloeosporioides pathogenicity by modulating the structural integrity of appressoria. [ABSTRACT FROM AUTHOR]