262 results on '"Fu-Rong Li"'
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2. 在中国非糖尿病或糖尿病患者中,用不同的方法评估肾小球滤过率和全因死亡率的风险:一项全国性的前瞻性研究
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Yu‐Jie Liu, Fu‐Rong Li, Cai‐Long Chen, Zhong‐Xiao Wan, Jin‐Si Chen, Jing Yang, Rong Liu, Jia‐Ying Xu, Yan Zheng, Li‐Qiang Qin, and Guo‐Chong Chen
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全因死亡率 ,慢性肾病 ,肌酐 ,胱抑素C ,糖尿病 ,估计肾小球滤过率 ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Abstract Background Whether estimated glomerular filtration rates (eGFRs) by differing biomarkers are differentially associated with mortality or whether the associations differ by diabetes status remains unclear, especially in Chinese population. Methods We included 6995 participants without diabetes (mean age: 60.4 years) and 1543 with diabetes (mean age: 61.8 years). Each eGFR measure was divided into normal (≥90 mL/min/1.73 m2), modestly declined (60 to
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- 2023
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3. 午睡与2型糖尿病风险之间的关联受到炎症和肥胖的调节:来自英国生物样本库(UK‐Biobank) 435 342名参与者的证据
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Rui Zhou, Hao‐Wen Chen, Yi‐Ning Huang, Qi Zhong, Fu‐Rong Li, Rui‐Dian Huang, Hua‐Min Liu, Jia‐Zhen Zheng, Jia‐Wen Xu, and Xian‐Bo Wu
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脂肪 ,队列研究 ,午睡 ,炎症 ,2型糖尿病 ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Abstract Background Existing evidence concerning the relationship between daytime napping and type 2 diabetes (T2D) is inconsistent, and whether the effects of napping differ by body fat percentage (BFP) and C‐reactive protein (CRP) is unclear. We aimed to investigate the association between daytime napping frequency and T2D risk and whether such an association was modified by BFP and CRP. Methods We included 435 342 participants free of diabetes from the UK Biobank. Participants were categorized as nonnappers, occasional nappers, and frequent nappers based on napping frequency, and BFP/CRP was divided into quartiles. Cox proportional hazards models were used. Results During a median follow‐up of 9.2 years, 17 592 T2D cases occurred. Higher frequency of daytime napping was significantly associated with an increased risk of T2D. Compared with nonnappers, the adjusted hazard ratios (HRs) for occasional nappers and habitual nappers were 1.28 (95% confidence interval [CI]: 1.24–1.32) and 1.49 (95% CI: 1.41–1.57), respectively. There was a significant additive and multiplicative interaction (relative excess risk due to interaction [RERI] = 0.490, 95% CI 0.307–0.673; p for multiplicative interaction
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- 2023
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4. Proteomic analysis reveals LRPAP1 as a key player in the micropapillary pattern metastasis of lung adenocarcinoma
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Hao-jie Yan, Sheng-cheng Lin, Shao-hang Xu, Yu-biao Gao, Bao-jin Zhou, Ruo Zhou, Fu-ming Chen, and Fu-rong Li
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Lung adenocarcinomas ,Micropapillary ,Metastasis ,LRPAP1 ,Proteomics ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Objectives: Lung adenocarcinomas have different prognoses depending on their histological growth patterns. Micropapillary growth within lung adenocarcinoma, particularly metastasis, is related to dismal prognostic outcome. Metastasis accounts for a major factor leading to mortality among lung cancer patients. Understanding the mechanisms underlying early stage metastasis can help develop novel treatments for improving patient survival. Methods: Here, quantitative mass spectrometry was conducted for comparing protein expression profiles among various histological subtypes, including adenocarcinoma in situ, minimally invasive adenocarcinoma, and invasive adenocarcinoma (including acinar and micropapillary [MIP] types). To determine the mechanism of MIP-associated metastasis, we identified a protein that was highly expressed in MIP. The expression of the selected highly expressed MIP protein was verified via immunohistochemical (IHC) analysis and its function was validated by an in vitro migration assay. Results: Proteomic data revealed that low-density lipoprotein receptor-related protein–associated protein 1 (LRPAP1) was highly expressed in MIP group, which was confirmed by IHC. The co-expressed proteins in this study, PSMD1 and HSP90AB1, have been reported to be highly expressed in different cancers and play an essential role in metastasis. We observed that LRPAP1 promoted lung cancer progression, including metastasis, invasion and proliferation in vitro and in vivo. Conclusion: LRPAP1 is necessary for MIP-associated metastasis and is the candidate novel anti-metastasis therapeutic target.
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- 2024
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5. Single-cell analyses reveal distinct expression patterns and roles of long non-coding RNAs during hESC differentiation into pancreatic progenitors
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Hai-Tao Luo, Qian He, Wei Yang, Fei He, Jun Dong, Chao-Feng Hu, Xiao-Fei Yang, Ning Li, and Fu-Rong Li
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hESC differentiation ,Pancreatic progenitors ,scRNA-seq ,LncRNA ,Metacell ,Functional annotation ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Deep understanding the differentiation process of human embryonic stem cells (hESCs) is essential for developing cell-based therapeutic strategy. Substantial efforts have been made to investigate protein-coding genes, yet it remains lacking comprehensive characterization of long non-coding RNAs (lncRNAs) during this process. Methods hESCs were passaged every 5–6 days and had maintained stable karyotype even until the 50th generation. Pancreatic progenitor specification of in vitro differentiation from hESCs was performed and modified. The nuclei were stained with 4,6-Diamidino-2-phenylindole (DAPI). Droplet-based platform (10X Genomics) was applied to generate the single-cell RNA sequencing (scRNA-seq) data. The quality of the filtered read pairs was evaluated by using FastQC. Batch effects were removed using the size factor method. Dimension reduction and unsupervised clustering analyses were performed using Seurat R package. The Monocle 2 and MetaCell algorithms were used to order single cells on a pseudotime course and partition the scRNA-seq data into metacells, respectively. Co-expression network was constructed using WGCNA. Module- and hub-based methods were adopted to predict the functions of lncRNAs. Results A total of 77,382 cells during the differentiation process of hESCs toward pancreatic progenitors were sequenced. According to the single-cell map, the cells from different time points were authenticated to constitute a relatively homogeneous population, in which a total of 7382 lncRNAs could be detected. Through further analyzing the time course data, conserved and specific expression features of lncRNAs during hESC differentiation were revealed. Based upon pseudotime analysis, 52 pseudotime-associated lncRNAs that grouped into three distinct expression patterns were identified. We also implemented MetaCell algorithm and network-based methods to explore the functional mechanisms of these lncRNAs. Totally, 464 lncRNAs, including 49 pseudotime-associated lncRNAs were functionally annotated by either module-based or hub-based methods. Most importantly, we demonstrated that the lncRNA HOTAIRM1, which co-localized and co-expressed with several HOX genes, may play crucial role in the generation of pancreatic progenitors through regulation of exocytosis and retinoic acid receptor signaling pathway. Conclusions Our single-cell analyses provide valuable data resources for biological researchers and novel insights into hESC differentiation processes, which will guide future endeavors to further elucidate the roles of lncRNAs.
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- 2023
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6. Zinc‐Organometallic Framework Vaccine Controlled‐Release Zn2+ Regulates Tumor Extracellular Matrix Degradation Potentiate Efficacy of Immunotherapy
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Lin Ding, Minli Liang, Yuanyuan Li, Mei Zeng, Meiting Liu, Wei Ma, Fuming Chen, Chenchen Li, Rui L. Reis, Fu‐Rong Li, and Yanli Wang
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immunotherapy ,tumor extracellular matrix ,vaccine ,zinc‐organometallic framework ,Zn2+ ,Science - Abstract
Abstract Tumor extracellular matrix (ECM) not only forms a physical barrier for T cells infiltration, but also regulates multiple immunosuppressive pathways, which is an important reason for immunotherapy failure. The cyclic guanosine monophosphate‐adenosine monophosphate synthase‐stimulator of interferon genes (cGAS‐STING) pathway plays a key role in activating CD8+ T cells, maintaining CD8+ T cells stemness and enhancing the antitumor effect. Herein, a zinc‐organometallic framework vaccine (ZPM@OVA‐CpG) prepared by self‐assembly, which achieves site‐directed release of Zn2+ in dendritic cell (DC) lysosomes and tumor microenvironment under acidic conditions, is reported. The vaccine actively targets DC, significantly enhances cGAS‐STING signal, promotes DC maturation and antigen cross‐presentation, and induces strong activation of CD8+ T cells. Meanwhile, the vaccine reaches the tumor site, releasing Zn2+, significantly up‐regulates the activity of matrix metalloproteinase‐2, degrades various collagen components of tumor ECM, effectively alleviates immune suppression, and significantly enhances the tumor infiltration and killing of CD8+ T cells. ZPM@OVA‐CpG vaccine not only solves the problem of low antigen delivery efficiency and weak CD8+ T cells activation ability, but also achieves the degradation of tumor ECM via the vaccine for the first time, providing a promising therapeutic platform for the development of efficient novel tumor vaccines.
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- 2023
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7. Total and Regional Fat/Muscle Mass Ratio and Risks of Incident Cardiovascular Disease and Mortality
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Rui Zhou, Hao‐Wen Chen, Yang Lin, Fu‐Rong Li, Qi Zhong, Yi‐Ning Huang, and Xian‐Bo Wu
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cardiometabolic ,cardiovascular disease ,fat/muscle ratio ,inflammatory ,mortality ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background To evaluate the sex‐specific associations of total and regional fat/muscle mass ratio (FMR) with cardiovascular disease (CVD) incidence and mortality, and to explore the underlying mechanisms driven by cardiometabolites and inflammatory cells. We compared the predictive value of FMRs to body mass index. Methods and Results This population‐based, prospective cohort study included 468 885 UK Biobank participants free of CVD at baseline. Fat mass and muscle mass were estimated using a bioelectrical impedance assessment device. FMR was calculated as fat mass divided by muscle mass in corresponding body parts (total body, trunk, arm, and leg). Multivariable Cox proportional hazards models and mediation analyses were used. During 12.5 years of follow‐up, we documented 49 936 CVD cases and 4158 CVD deaths. Higher total FMR was associated with an increased risk of incident CVD (hazard ratios [HRs] were 1.63 and 1.83 for men and women, respectively), ischemic heart disease (men: HR, 1.61; women: HR, 1.81), myocardial infarction (men: HR, 1.72; women: HR, 1.49), and congestive heart failure (men: HR, 2.25; women: HR, 2.57). The positive associations of FMRs with mortality from total CVD or its subtypes were significant mainly in trunk and arm for male patients (P for trend
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- 2023
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8. Shift work and risk of incident gastroesophageal reflux disease: the association and mediation
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Qian Li, Fu-Rong Li, Shihan Zhen, Jian Liao, Keye Wu, Xia Li, Bincai Wei, Zhiyi Xiao, Qingyao Wu, Xian-Bo Wu, and Fengchao Liang
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shift work ,gastroesophageal reflux disease ,cohort study ,mediation ,public health ,risk factors ,Public aspects of medicine ,RA1-1270 - Abstract
IntroductionShift work has become an increasingly common work mode globally. This study aimed to investigate the association between shift work and the risk of incident gastroesophageal reflux disease (GORD), an upward gastrointestinal disorder disease worldwide, and to explore the mediating factors.MethodA total of 262,722 participants from the UK Biobank free of GORD and related gastrointestinal diseases were included to investigate the association and potential mediators between shift work and incident GORD. Multivariate-adjusted Cox models were used to evaluate the association between shift work status and GORD incidence.ResultsCompared to non-shift workers, shift workers had a 1.10-fold greater risk of incident GORD [95% confidence intervals (CIs): 1.03, 1.18], after adjusting for a range of potential confounders. However, the excess risk of GORD attenuated to the null after further adjusting for selected mediators. Specifically, the association was mediated by sleep patterns (25.7%), healthy behaviors (16.8%), depressive symptoms (20.2%), chronic conditions (13.3%), and biological factors (17.6%). After adjustment for all the mediators together, the association was attenuated by 71.5%.DiscussionOur findings indicated that long-term shift workers may have a higher risk of incident GORD, yet the excess risk may be explained by poor sleep quality, unhealthy behaviors, depressive symptoms, etc. This has positive implications for protecting the health of shift workers.
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- 2023
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9. Waist Circumference and Body Mass Index Variability and Incident Diabetic Microvascular Complications: A Analysis of ACCORD Trial
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Daniel Nyarko Hukportie, Fu-Rong Li, Rui Zhou, Jia-Zhen Zheng, Xiao-Xiang Wu, and Xian-Bo Wu
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body mass index ,diabetes complications ,diabetes mellitus, type 2 ,obesity ,waist circumference ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Background Obesity is associated with adverse health events among diabetic patients, however, the relationship between obesity fluctuation and risk of microvascular complications among this specific population is unclear. We aimed to examine the effect of waist circumference (WC) and body mass index (BMI) variability on the risk of diabetic microvascular outcome Methods Annually recorded anthropometric data in the Action to Control Cardiovascular Risk in Diabetes (ACCORD) study was used to examine the association of WC and BMI variability defined as variability independent of mean, with the risk of microvascular outcomes, including neuropathy, nephropathy, and retinopathy. Cox proportional hazards models were used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) (Trial registration: ClinicalTrials.gov., no. NCT00000620). Results There were 4,031, 5,369, and 2,601 cases of neuropathy, nephropathy, and retinopathy during a follow-up period of 22,524, 23,941, and 23,850 person-years, respectively. Higher levels of WC and BMI variability were associated with an increased risk of neuropathy. Compared with the lowest quartile, the fully-adjusted HR (95% CI) for the highest quartile of WC and BMI variability for neuropathy risk were 1.21 (1.05 to 1.40) and 1.16 (1.00 to 1.33), respectively. Also, higher quartiles of BMI variability but not WC variability were associated with increased risk of nephropathic events. The fully-adjusted HR (95% CI) for the highest quartile compared with the lowest quartile of BMI variability was 1.31 (1.18 to 1.46). However, the results for retinopathic events were all insignificant. Conclusion Among participants with type 2 diabetes mellitus, WC and BMI variability were associated with a higher risk of neuropathic events, whereas BMI variability was associated with an increased risk of nephropathic events.
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- 2022
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10. Lipoprotein(a), family history of cardiovascular disease, and incidence of heart failure
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Hai-Peng Wang, Na Zhang, Yu-Jie Liu, Tian-Long Xia, Guo-Chong Chen, Jing Yang, and Fu-Rong Li
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family history of cardiovascular disease ,heart failure ,Lipoprotein(a) ,Biochemistry ,QD415-436 - Abstract
Lipoprotein(a) (Lp(a)) is a largely genetically determined biomarker for cardiovascular disease (CVD), while its potential interplay with family history (FHx) of CVD, a measure of both genetic and environmental exposures, remains unclear. We examined the associations of Lp(a) in terms of circulating concentration or polygenetic risk score (PRS), and FHx of CVD with risk of incident heart failure (HF). Included were 299,158 adults from the UK Biobank without known HF and CVD at baseline. Hazards ratios (HRs) and 95% Cls were estimated by Cox regression models adjusted for traditional risk factors defined by the Atherosclerosis Risk in Communities study HF risk score. During the 11.8-year follow-up, 5,502 incidents of HF occurred. Higher levels of circulating Lp(a), Lp(a) PRS, and positive FHx of CVD were associated with higher risks of HF. Compared with individuals who had lower circulating Lp(a) and no FHx, HRs (95% CIs) of HF were 1.36 (1.25, 1.49), 1.31 (1.19, 1.43), and 1.42 (1.22, 1.67) for those with higher Lp(a) and a positive history of CVD for all family members, parents, and siblings, respectively; similar results were observed by using Lp(a) PRS. The risk estimates for HF associated with elevated Lp(a) and positive FHx were attenuated after excluding those with incident myocardial infarction (MI) during follow-up. Lp(a) and FHx of CVD were independent risk factors for incident HF, and the highest risk of HF was observed among individuals with both risk factors. The association may be partly mediated by myocardial infarction.
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- 2023
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11. 控制糖尿病心血管风险的行动(ACCORD)试验中的血脂变异性和微血管并发症风险:一项事后分析
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Daniel Nyarko Hukportie, Fu‐Rong Li, Rui Zhou, Jia‐Zhen Zheng, Xiao‐Xiang Wu, Meng‐Chen Zou, and Xian‐Bo Wu
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高密度脂蛋白 ,低密度脂蛋白 ,甘油三酯 ,残余胆固醇 ,微血管并发症 ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Abstract Background Greater lipid variability may cause adverse health events among diabetic patients. We aimed to examine the effect of lipid variability on the risk of diabetic microvascular outcomes among type 2 diabetes mellitus patients. Methods We assessed the association between visit‐to‐visit variability (measured by variability independent of mean) in high‐density lipoprotein (HDL) cholesterol, low‐density lipoprotein‐cholesterol (LDL), triglyceride, and remnant cholesterol (RC) measurements among participants involved in the Action to Control Cardiovascular Risk in Diabetes (ACCORD) study and the risk of incident microvascular outcomes, including nephropathy, neuropathy, and retinopathy. Cox proportional hazards models were used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs), adjusted for potential confounders. Results There were 2400, 2470, and 2468 cases of nephropathy, neuropathy, and retinopathy during a follow‐up period of 22 600, 21 542, and 26 701 person‐years, respectively. Higher levels of HDL, triglyceride, and RC variability were associated with an increased risk of incident nephropathy and neuropathy. Compared with the lowest quartile, the fully adjusted HRs (95% CI) for the highest quartile of HDL, triglyceride, and RC variability for nephropathy risk were 1.57 (1.22, 2.01), 1.50 (1.18, 1.92), and 1.40 (1.09, 1.80), respectively; and for neuropathy, the corresponding risks were 1.36 (1.05, 1.75), 1.47 (1.14, 1.91), and 1.35 (1.04, 1.74), respectively. Null association was observed between LDL variability and all microvascular complications. Additionally, all associations of variability in the other lipids with retinopathy risk were null. Conclusion Among individuals with type 2 diabetes mellitus, HDL, triglyceride, and RC variability were associated with increased risks of nephropathy and neuropathy but not retinopathy. Trial registration: ClinicalTrials.gov., no. NCT00000620.
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- 2022
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12. Long-Term Visit-To-Visit Blood Pressure Variability and Risk of Diabetes Mellitus in Chinese Population: A Retrospective Population-Based Study
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Rui Zhou, Fu-Rong Li, Kuan Liu, Rui-Dian Huang, Hua-Min Liu, Ze-Lin Yuan, Jia-Zhen Zheng, Meng-Chen Zou, and Xian-Bo Wu
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China ,diabetes mellitus ,blood pressure ,variability ,cohort ,Public aspects of medicine ,RA1-1270 - Abstract
Objectives: To examine the association between visit-to-visit blood pressure variability (BPV) and incident diabetes mellitus (DM) risk in a Chinese population.Methods: Data comes from China Health and Nutrition Survey (n = 15,084). BPV was estimated as the average real variability (ARV) using at least three BP measurements from the year preceding the event and was divided into quartiles. Participants were also categorized into 9 groups on the basis of combinations of systolic BPV (SBPV) and diastolic BPV (DBPV) tertiles. Cox proportional hazards regression models were used.Results: During a median follow-up of 16.8 years, 1,030 (6.8%) participants developed diabetes (incidence rate: 4.65/1,000 person-years). The HRs (95% CIs) for the highest quartile (vs. the lowest quartile) of SBPV and DBPV were 1.60 (1.30–1.97) and 1.37 (1.13–1.67), respectively. Participants with both highest SBPV and DBPV tertile had an ≈89% higher risk of DM (HR, 1.89; 95% CI, 1.47–2.42) compared with those in the both SBPV and DBPV tertile 1 group.Conclusion: Higher SBP ARV and DBP ARV were independently associated with increased risk of incident DM, which was augmented when both presented together.
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- 2023
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13. Integrative analysis reveals a lineage-specific circular RNA landscape for adipo-osteogenesis of human mesenchymal stem cells
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Hai-Bo Huang, Hai-Tao Luo, Na-Na Wei, Miao-Ling Liu, Fei He, Wei Yang, Jun Dong, Xiao-Fei Yang, and Fu-Rong Li
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Mesenchymal stem cells ,Osteogenesis ,Adipogenesis ,Circular RNAs ,CRLF1 ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background The balance between osteogenesis and adipogenesis of mesenchymal stem cells (MSCs) is critical to skeletal development and diseases. As a research hotspot, circular RNAs (circRNAs) have expanded our understanding of a hidden layer of the transcriptome. Yet, their roles during adipo-osteogenesis remain poorly described. Methods The identity of human MSCs derived from bone marrow and adipose were first determined by flow cytometry, cellular staining, and quantitative polymerase chain reaction (qPCR). Multi-strategic RNA-sequencing was performed using Poly A, RiboMinus and RiboMinus/RNase R methods. Integrative analysis was performed to identify lineage-specific expressed circRNAs. The structural and expressional characteristics were identified by Sanger sequencing and qPCR, respectively. The regulatory effects of adipogenesis-specific circ-CRLF1 were confirmed using siRNA transcfection and qPCR. Results We generated a whole transcriptome map during adipo-osteogenesis based on 10 Poly A, 20 RiboMinus and 20 RiboMinus/ RNase R datasets. A total of 31,326 circRNAs were identified and quantified from ~ 3.4 billion paired-end reads. Furthermore, the integrative analysis revealed that 1166 circRNA genes exhibited strong lineage-specific expression patterns. Their host genes were enriched in distinct biological functions, such as cell adhesion, cytokine signaling, and cell division. We randomly selected and validated the back-spliced junction sites and expression patterns of 12 lineage-specific circRNAs. Functional analysis indicated that circ-CRLF1 negatively regulated adipogenesis. Conclusions Our integrative analysis reveals an accurate and generally applicable lineage-specific circRNA landscape for adipo-osteogenesis of MSCs and provides a potential therapeutic target, circ-CRLF1, for the treatment of skeleton-related disease.
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- 2022
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14. Prevalence and associated factors of sexually transmitted infections among methamphetamine users in Eastern China: a cross-sectional study
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Xing Ye, Fu-Rong Li, Qing Pan, Zhen Li, Gong-Qi Yu, Hong Liu, Jian Liu, Peng-Cheng Huai, and Fu-Ren Zhang
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Methamphetamine ,STIs ,Prevalence ,Associated factors ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background The reported incidence of sexually transmitted infections (STIs) in China has been increasing over the last decades, especially among drug users, which has become one of the main burdens of public health in China. This study was conducted to estimate the prevalence and associated factors of STIs among non-injecting methamphetamine (MA) users in Eastern China. Methods A cross-sectional survey was conducted among 632 MA users in Eastern China in 2017. Demographic characteristics, sexual behaviors, behaviors of MA use and sexual health knowledge were collected through questionnaire. First pass urine specimens were collected and detected for deoxyribonucleic acid (DNA) of Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) with Nucleic Acid Amplification Technology (NAAT), while blood specimens were collected and detected for antibodies of Human immunodeficiency virus (HIV), Herpes simplex virus type-2 (HSV-2), and syphilis with enzyme-linked immune sorbent assay (ELISA). Results Among the 632 MA users, 464 (73.42%) were males, 60.92% were
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- 2022
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15. Influence of hypertension duration and blood pressure levels on cardiovascular disease and all-cause mortality: A large prospective cohort study
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Yan Zheng, Xiang Gao, Hai-Yi Jia, Fu-Rong Li, and Hui Ye
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hypertension duration ,blood pressure ,cardiovascular disease ,mortality ,cohort ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background and objectsA longer duration of hypertension (HTN) has been suggested to be associated with a greater risk of cardiovascular disease (CVD). Whether such an association is similar for mortality risk, and whether HTN duration is associated with CVD/mortality beyond blood pressure (BP) control levels are yet to be assessed. We aimed to examine the associations of HTN duration and the combination of HTN duration and systolic blood pressure (SBP)/diastolic blood pressure (DBP) with risks of CVD and all-cause mortality.MethodsWe used data on ∼450,000 UK residents. Participants were categorized by HTN status and HTN duration. The primary outcome was a composite of non-fatal myocardial infarction, non-fatal stroke and CVD death. We also explored the results for the above-mentioned CVD outcomes separately. All-cause mortality was also used as a secondary outcome. The age at HTN diagnosis was obtained by self-report. HTN duration was calculated as baseline age minus age at diagnosis.ResultsAmong all participants, compared with non-hypertensive participants, those with a longer HTN duration had increased risks of CVD and all-cause mortality. These associations persisted among hypertensive patients. Specifically, compared with patients with HTN durations of < 5 y, patients with a HTN duration of 5 to < 10 y, 10 to < 15 y, and ≥ 15 y had adjusted HRs (95% CI) of 1.09 (1.03, 1.17), 1.21 (1.13, 1.31), and 1.38 (1.29, 1.48) for composite CVD (P-trend < 0.001); and 1.03 (0.97, 1.08), 1.09 (1.02, 1.16), and 1.17 (1.11, 1.24) for all-cause mortality (P-trend < 0.001). When compared with hypertensive patients with BP < 140/90 mmHg and a HTN duration of < 5 y, adjusted HRs of CVD and all-cause mortality were 1.35 (1.15, 1.57) and 1.26 (1.11, 1.42) for those with BP < 140/90 mmHg and a duration of ≥ 15 y, and 1.43 (1.26, 1.60) and 1.13 (1.03, 1.25) for those with BP ≥ 140/90 mmHg and durations of ≥ 15 y, respectively.ConclusionA longer HTN duration was associated with increased risks of CVD and overall death in a linear fashion, and these associations were independent of BP control levels.
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- 2022
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16. Long-term exposure to air pollution and risk of incident inflammatory bowel disease among middle and old aged adults
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Fu-Rong Li, Ke-Yi Wu, Wei-Dong Fan, Guo-Chong Chen, Haili Tian, and Xian-Bo Wu
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Inflammatory bowel disease ,Air pollution ,Epidemiology ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
Background: Epidemiological evidence regarding the associations between long-term exposure to air pollution and risk of incident inflammatory bowel disease (IBD) is scant. Objectives: We examined the associations of various specific air pollutants with the risk of incident ulcerative colitis and Crohn’s disease, two subtypes of IBD, among middle and old aged adults in the UK. We also explored potential susceptible subgroups. Methods: We used data from the UK Biobank study. Information on air pollution, including PM2.5, PM2.5–10, PM10 as well as NO2 and NOx were estimated using the Land Use Regression model. Multivariable Cox proportional hazards models were used to calculate hazard ratios (HRs) and 95% confidence intervals (CIs). Results: After a median follow-up of 11.7 years, 1872 incident ulcerative colitis and 865 incident Crohn’s disease cases were identified among 455,210 IBD-free participants. HRs (95% CIs) of ulcerative colitis associated with each 1 interquartile range (IQR) increase in PM2.5, PM2.5–10, PM10, NO2, and NOx were 1.06 (1.01, 1.12), 1.03 (0.99, 1.08), 1.09 (1.03, 1.16), 1.12 (1.07, 1.19), and 1.07 (1.02, 1.12), respectively. The associations between all the air pollutants and risk of Crohn’s disease were null. Smoking status and sex appeared to respectively modify the associations between some air pollutants and risk of ulcerative colitis and Crohn’s disease. Conclusion: Long-term exposure to various air pollutants was associated with the risk of incident ulcerative colitis but not Crohn’s disease, highlighting the importance of developing environmental health strategy to reduce the burden of ulcerative colitis.
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- 2022
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17. Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells
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Hai-Tao Luo, Yuan-Yuan Zheng, Jun Tang, Li-Juan Shao, Yi-Heng Mao, Wei Yang, Xiao-Fei Yang, Yang Li, Rui-Jun Tian, and Fu-Rong Li
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Medicine ,Genetics ,QH426-470 - Abstract
Abstract Lung adenocarcinoma is heterogeneous and hierarchically organized, with a subpopulation of stem-like cells (CSCs) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have been numerous studies on tumor exosomes, the constituents and functional properties of CSC-derived exosomes are still poorly characterized. Here we present a detail transcriptome and proteome atlas of the exosomes released by human lung adenocarcinoma stem-like cells (LSLCs). The transcriptome analysis indicates the specific patterns of exosomal constituents, including the fragmentation of transcripts and the low-level presence of circular RNAs, and identifies multiple exosomal-enriched mRNAs and lncRNAs. Integrative analysis of transcriptome and proteome data reveals the diverse functions of exosomal-enriched RNAs and proteins, many of which are associated with tumorigenesis. Importantly, several LSLC markers we identified are highly expressed in LSLC-derived exosomes and associate with poor survival, which may serve as promising liquid biopsy biomarkers for lung adenocarcinoma diagnosis. Our study provides a resource for the future elucidation of the functions of tumor-derived exosomes and their regulatory mechanisms in mediating lung cancer development.
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- 2021
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18. Waist Circumference and Body Mass Index Variability and Incident Diabetic Microvascular Complications: A Analysis of ACCORD Trial ( 2022;46:767-80)
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Daniel Nyarko Hukportie, Fu-Rong Li, Rui Zhou, Jia-Zhen Zheng, Xiao-Xiang Wu, and Xian-Bo Wu
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Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Published
- 2023
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19. Association of regular aerobic exercises and neuromuscular junction variants with incidence of frailty: an analysis of the Chinese Longitudinal Health and Longevity Survey
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Yu‐Jie Zhang, Yao Yao, Pei‐Dong Zhang, Zhi‐Hao Li, Pei Zhang, Fu‐Rong Li, Zheng‐He Wang, Dan Liu, Yue‐Bin Lv, Lin Kang, Xiao‐Ming Shi, and Chen Mao
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regular aerobic exercises ,neuromuscular junction ,frailty ,Diseases of the musculoskeletal system ,RC925-935 ,Human anatomy ,QM1-695 - Abstract
Abstract Background Candidate genes of neuromuscular junction (NMJ) pathway increased risk of frailty, but the extent and whether can be offset by exercises was unclear. The aim of this study was to investigate the association between aerobic exercises and incident frailty regardless of NMJ pathway‐related genetic risk. Methods A cohort study on participants from Chinese Longitudinal Healthy Longevity Survey was conducted from 2008 to 2011. A total of 7006 participants (mean age of 80.6 ± 10.3 years) without frailty at baseline were interviewed to record aerobic exercise status, and 4053 individuals among them submitted saliva samples. NMJ pathway‐related genes were genotyped and weighted genetic risk scores were constructed. Results During a median follow‐up of 3.1 years (19 634 person‐years), there were 1345 cases (19.2%) of incident frailty. Persistent aerobic exercises were associated with a 26% lesser frailty risk [adjusted hazard ratio (HR) = 0.74, 95% confidence interval (CI) = 0.64–0.85]. This association was stronger in a subgroup of 1552 longevous participants (age between 90 and 111 years, adjusted HR = 0.72, 95% CI = 0.60–0.87). High genetic risk was associated with a 35% increased risk of frailty (adjusted HR = 1.35, 95% CI = 1.16–1.58). Of the participants with high genetic risk and no persistent aerobic exercises, there was a 59% increased risk of frailty (adjusted HR = 1.59, 95% CI = 1.20–2.09). HRs for the risk of frailty increased from the low genetic risk with persistent aerobic exercise to high genetic risk without persistent aerobic exercise (P trend
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- 2021
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20. Associations Between High-Sensitivity C-Reactive Protein and All-Cause Mortality Among Oldest-Old in Chinese Longevity Areas: A Community-Based Cohort Study
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Pei-Liang Chen, Zhi-Hao Li, Hai-Lian Yang, Zhao-Jin Cao, Xin Cheng, Feng Zhao, Xi-Ru Zhang, Yue-Bin Lv, Fu-Rong Li, Yuan-Feng Zhou, Hao-Nan Li, Ying-Li Qu, Zhao-Xue Yin, Ling Liu, Xian-Bo Wu, Xiao-Ming Shi, and Chen Mao
- Subjects
high-sensitivity C-reactive protein ,all-cause mortality ,oldest-old ,inflammation ,aging ,cohort study ,Public aspects of medicine ,RA1-1270 - Abstract
BackgroundThe association between high-sensitivity C-reactive protein (hsCRP) levels and all-cause mortality for the oldest-old (aged 80 years or older) remains unclear. We aimed to investigate the associations between hsCRP concentrations and the risks of all-cause mortality, and further identify the potential modifying factors affecting these associations among the oldest-old.MethodsThis prospective, community-based cohort study included 2,206 participants aged 80 years or older (median age 93.0 years) from the Healthy Aging and Biomarkers Cohort Study. Cox proportional hazards regression models were used to estimate hazard ratios (HRs) with 95% confidential intervals (95% CIs) for all-cause mortality according to hsCRP quartiles and recommendation for relative risk categories of hsCRP levels (< 1.0, 1.0–3.0, and > 3.0 mg/L), with adjustment for sociodemographic information, lifestyle, physical examination, medical history, and other potential confounders.ResultsDuring a median follow-up period of 3.1 years (IQR: 1.6–3.9 years), 1,106 deaths were verified. After full adjustment for potential confounders, a higher hsCRP concentration was positively associated with an increased risk of all-cause mortality (P for trend < 0.001). Compared with the lowest quartile, the fully adjusted HRs of the second, third, and fourth quartiles were 1.17 (95% CI: 0.94, 1.46), 1.28 (95% CI: 1.01, 1.61), and 1.49 (95% CI: 1.20, 1.87), respectively. The association of hsCRP with all-cause mortality was modified by smoking status (P for interaction = 0.011), an increased risk of hsCRP with all-cause mortality showed among non-current smokers (HR: 1.17; 95% CI: 1.07, 1.28), but no significance was observed in current smokers (HR: 0.83; 95% CI: 0.66, 1.18).ConclusionsOur study indicated that elevated hsCRP concentrations were associated with a higher risk of all-cause mortality among Chinese oldest-old. Future studies investigating additional factors of disease and aging processes are needed to obtain a better understanding of the mechanisms.
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- 2022
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21. Inhibition of LSD1 promotes the differentiation of human induced pluripotent stem cells into insulin-producing cells
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Xiao-Fei Yang, Shu-Yan Zhou, Ce Wang, Wei Huang, Ning Li, Fei He, and Fu-Rong Li
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LSD1 ,Human induced pluripotent stem cells ,Insulin-producing cells ,Differentiation ,Diabetes ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Human induced pluripotent stem cells (hiPSCs) represent a potentially unlimited source of pancreatic endocrine lineage cells. Although insulin-producing β cells derived from hiPSCs have been successfully induced, much work remains to be done to achieve mature β cells. Lysine-specific demethylase 1 (LSD1) plays an important role in the regulation of hiPSC self-renewal and differentiation. We propose a new strategy to acquire insulin-producing cells (IPCs) from hiPSCs by knocking down LSD1. Methods Knockdown of LSD1 in hiPSCs with five shRNA. Assessment of the effects of shRNA on hiPSC proliferation, cell cycle, and apoptosis. Using knockdown hiPSCs with 31.33% LSD1 activity, we achieved a four-step differentiation into IPCs and test its differentiation efficiency, morphology, and marker genes and proteins. We implanted the IPCs into the renal subcapsular of SCID-Beige diabetic mice to evaluate the hypoglycemic effect in vivo. We tested LSD1 and HDAC1 whether they are present in the CoREST complex through IP-WB, and analyzed LSD1, CoREST, HDAC1, H3K4me2/me3, and H3K27me3 protein expression before and after knockdown of LSD1. Results Differentiated hiPSCs were 38.32% ± 3.54% insulin-positive cells and released insulin/C-peptide in response to glucose stimulus in a manner comparable to adult human islets. Most of the IPCs co-expressed mature β cell-specific markers. When transplanted under the left renal capsule of SCID-Beige diabetic mice, these IPCs reversed hyperglycemia, leading to a significant increase in the definitive endoderm cells. IP-WB results showed that LSD1, HDAC1, and CoREST formed a complex in hiPSCs. Chip-PCR results showed that LSD1, HDAC1, and CoREST were enriched in the same district during the SOX17 and FOXA2 promoter region. Inhibition of LSD1 would not affect the level of CoREST but decreased the HDAC1 expressions. The H3K4me2/me3 and H3K9act level of SOX17 and FOXA2 promoter region increased after inhibited of LSD1, and promoted transcriptional activation. The H3K4me2/me3 and H3K9act level of OCT4 and SOX2 promoter region decreased with the transcriptional repressed. Conclusions LSD1 regulated histone methylation and acetylation in promoter regions of pluripotent or endodermal genes. Our results suggest a highly efficient approach to producing IPCs from hiPSCs.
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- 2020
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22. LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells
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Fei He, Ning Li, Hai-Bo Huang, Jing-Bo Wang, Xiao-Fei Yang, Hua-Dong Wang, Wei Huang, and Fu-Rong Li
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Human embryonic stem cells ,Differentiation ,Insulin-producing cells ,Lysine-specific demethylase 1(LSD1) ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Human embryonic stem cells represent a potentially unlimited source of insulin-producing cells for diabetes therapy. While tremendous progress has been made in directed differentiation of human embryonic stem cells into IPCs in vitro, the mechanisms controlling its differentiation and function are not fully understood. Previous studies revealed that lysine-specific demethylase 1(LSD1) balanced the self-renewal and differentiation in human induced pluripotent stem cells and human embryonic stem cells. This study aims to explore the role of LSD1 in directed differentiation of human embryonic stem cells into insulin-producing cells. Methods Human embryonic stem cell line H9 was induced into insulin-producing cells by a four-step differentiation protocol. Lentivirus transfection was applied to knockdown LSD1 expression. Immunofluorescence assay and flow cytometry were utilized to check differentiation efficiency. Western blot was used to examine signaling pathway proteins and differentiation-associated proteins. Insulin/C-peptide release was assayed by ELISA. Statistical analysis between groups was carried out with one-way ANOVA tests or a student’s t test when appropriate. Results Inhibition or silencing LSD1 promotes the specification of pancreatic progenitors and finally the commitment of functional insulin-producing β cells; Moreover, inhibition or silencing LSD1 activated ERK signaling and upregulated pancreatic progenitor associated genes, accelerating pre-maturation of pancreatic progenitors, and conferred the NKX6.1+ population with better proliferation ability. IPCs with LSD1 inhibitor tranylcypromine treatment displayed enhanced insulin secretion in response to glucose stimulation. Conclusions We identify a novel role of LSD1 inhibition in promoting IPCs differentiation from hESCs, which would be emerged as potential intervention for generation of functional pancreatic β cells to cure diabetes.
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- 2020
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23. Tissue microRNAs in non-small cell lung cancer detected with a new kind of liquid bead array detection system
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Yuan-Yuan Zheng, Yun Fei, Zheng Wang, Yue Chen, Cheng Qiu, and Fu-Rong Li
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miRNAs ,Liquid bead array ,Lung cancer ,Tumor tissue ,Medicine - Abstract
Abstract Background Commonly used miRNA detection methods cannot be applied for high-throughput analyses. However, this study was aimed to performed a liquid bead array detection system (LBAS) to detect tissue 6 miRNAs in non-small cell lung cancer (NSCLC). Methods In this study, evaluation of LBAS was performed to observe the precision, specificity, limitation and stability. Then, a total of 52 primary NSCLC patients who received resection operation without preoperative radiotherapy and chemotherapy between June 2013 and March 2014 were selected, and then the total RNA of the tissues were extracted. We prepared six NSCLC-related miRNAs for LBAS. After optimization and evaluation, LBAS was verified by detecting the relative expression levels of 6 microRNAs in the pathological tissues and corresponding normal tissues of 52 NSCLC patients. Results The results of evaluation of LBAS showed that the Mean Fluorescence Intensity (MFI) of the reaction only added with chimeric probes and beads showed no significant change after 180 days (P > 0.05). And the intra-assay Coefficient of Variation (CV) was between 1.57 and 3.5%, while the inter-assay CV was between 4.24 and 11.27%, indicating this system was ideal for diagnostic reagents. In addition, only the beads corresponding to the additional miRNAs showed high MFIs from 8426 to 18,769, whereas the fluorescence values of the other beads were under background levels (MFIs = 20 to 55) in each reaction, indicating no cross reactivity among the miRNAs. The limit of detection of miR-21, miR-210, miR-125b, miR-155, miR-375, and miR-31 were 5.27, 1.39, 1.85, 2.01, 1.34, and 2.73 amol/μL, respectively, showing that the lowest detection limit of miRNA by this system was under pM level. Then, the relative expression levels of miR-21, miR-210, miR-125b, miR-155, miR-375, and miR-31 by using this system were significantly correlated with NSCLC (P
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- 2020
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24. Relationship Between Fish Oil Use and Incidence of Primary Liver Cancer: Findings From a Population-Based Prospective Cohort Study
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Wei Jiang, Fu-Rong Li, Huan-Huan Yang, Guo-Chong Chen, and Yong-Fei Hua
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fish ,fish oil ,hepatocellular carcinoma ,liver cancer ,polyunsaturated fatty acids ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Background: N-3 long-chain polyunsaturated fatty acids (LCPUFAs) prevented non-alcoholic steatohepatitis (NASH) and hepatocellular carcinoma (HCC) in studies of mouse models. We examined prospective relationships between fish oil use and risk of primary liver cancer and the major histological subtypes, such as HCC and intrahepatic cholangiocarcinoma (ICC).Methods: We included 434,584 middle-aged and older men and women who were free of cancer at recruitment of the UK Biobank (2006–2010). Information on fish oil use and other dietary habits was collected via questionnaires. Cox proportional hazards models were used to compute the hazard ratio (HR) and 95% CI of liver cancer associated with fish oil use, with adjustment for socio-demographic, lifestyle, dietary, and other clinical risk factors.Results: At baseline, 31.4% of participants reported regular use of fish oil supplements. During a median of 7.8 years of follow-up, 262 incident liver cancer cases were identified, among which 127 were HCC and 110 were ICC cases. As compared with non-users, fish oil users had a significantly 44% (95% CI: 25–59%) lower risk of total liver cancer, and 52% (95% CI: 24–70%) and 40% (95% CI: 7–61%) lower risk of HCC and ICC, respectively. Higher intake of oily fish also was associated with a lower risk of HCC (≥2 vs.
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- 2021
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25. Association of Predicted Lean Body Mass and Fat Mass With Incident Diabetic Nephropathy in Participants With Type 2 Diabetes Mellitus: A Post Hoc Analysis of ACCORD Trial
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Daniel Nyarko Hukportie, Fu-Rong Li, Rui Zhou, Meng-Chen Zou, Xiao-Xiang Wu, and Xian-Bo Wu
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obesity ,lean body mass ,fat mass ,diabetes ,diabetic nephropathy ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
BackgroundLean body mass (LBM) and fat mass (FM) have been shown to have different associations with several chronic diseases but little is known about the sex-specific association of LBM and FM with diabetic nephropathy (DN) risk among participants with diabetes.MethodsParticipants from the Action to Control Cardiovascular Risk in Diabetes (ACCORD) study was used in a post hoc analysis to examine the association of predicted LBM index (LBMI) and FM index (FMI) with incident DN risk (defined as a composite outcome of three types of predefined DN). Because of sex differences in body composition, analyses were conducted separately using sex-specific quartiles of predicted LBMI and FMI.ResultsOf the 9,022 participants with type 2 diabetes (5,575 men and 3,447 women) included in this study, 5,374 individuals developed DN (3,396 in men and 1,978 in women). Higher quartiles of LBMI were associated with a reduced risk of DN while higher quartiles of FMI were associated with an increased higher risk of DN among men but not women. Compared with the lowest quartile, the fully adjusted hazard ratios (HRs) and 95% confidence intervals (CIs)for the highest quartile of predicted LBMI and FMI were respectively 0.83 (95% CI 1.71 – 0.96) and 1.23 (95% CI 1.06-1.43) among men; and 0.92 (95% CI 0.63 – 1.33) and 1.14 (95% CI 0.79 – 1.63) among women.ConclusionsAmong participants with diabetes, predicted LBMI was inversely associated with risk of DN while predicted FMI was positively associated with an increased risk of incident DN among men but not women. Trial registration: ClinicalTrials.gov., no. NCT00000620.
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- 2021
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26. Anthropometric Measures and Incident Diabetic Nephropathy in Participants With Type 2 Diabetes Mellitus
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Daniel Nyarko Hukportie, Fu-Rong Li, Rui Zhou, Jia-Zhen Zheng, Xiao-Xiang Wu, and Xian-Bo Wu
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obesity ,waist circumference ,waist-to-height ratio ,body mass index ,diabetic nephropathy ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
BackgroundThe prevalence of diabetes is on the rise globally coupled with its associated complications, such as diabetic nephropathy (DN). Obesity has been identified as a risk factor for the development of DN but it is still unclear which obesity index is the best predictor of incident DN.MethodsData from the participants with type 2 diabetes mellitus (T2DM) in the Action to Control Cardiovascular Risk in Diabetes (ACCORD) study were used to examine the sex-specific association between waist circumference (WC), waist-to-height ratio (WHtR), and body mass index (BMI) with incident DN risk.ResultsAmong the 8,887 participants with T2DM (5,489 men and 3,398 women), 5,296 participants (3,345 men and 1,951 women) developed the DN composite outcome during a follow-up period of 24302 person-years. Among men, null associations were observed between all anthropometric measures with incident DN in the multivariate analysis although the 3rd quartile of WHtR showed marginally significant results (P = 0.052). However, among women, both central and general obesity measures were associated with increased risks of incident DN. Compared with participants in the WC
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- 2021
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27. Association between coffee consumption and risk of bladder cancer in a meta-analysis of 16 prospective studies
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Zhi-Wei Dai, Ke-Dan Cai, Fu-Rong Li, Xian-Bo Wu, and Guo-Chong Chen
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Coffee ,Bladder cancer ,Cohort studies ,Meta-analysis ,Nutrition. Foods and food supply ,TX341-641 ,Nutritional diseases. Deficiency diseases ,RC620-627 - Abstract
Abstract Background Current evidence remains equivocal as to whether and how consumption of coffee may be associated with risk of bladder cancer, and potential influence of confounding by smoking on this association is yet to be elucidated. We conducted an updated meta-analysis of prospective studies to address these issues. Methods Relevant studies were identified by searching PubMed and EMBASE databases from inception to April 2019. A random-effects model was used to estimate summary relative risk (RR) with corresponding 95% confidence interval (CI) of bladder cancer associated with coffee consumption. Results The final analysis included 16 prospective studies comprising 2,122,816 participants and 11,848 bladder cancer cases. Overall, coffee consumption was not associated with risk of bladder cancer (RR high-vs-low = 1.07, 95% CI: 0.96–1.20). The lack of association persisted in the strata defined by sex or participants’ smoking status. Meta-regression analyses identified the number cases (P difference = 0.06) and the degree of adjustment for smoking (P difference = 0.04) as potential sources of heterogeneity. There was an increased risk of bladder cancer related to higher coffee consumption among studies with fewer cases (RR high-vs-low = 1.38, 95% CI: 1.05–1.81) and among those with poorer adjustment for smoking (RR high-vs-low = 1.48, 95% CI: 1.14–1.93). Results were similar in the dose-response analyses (RR 1 cup/d = 1.01, 95% CI: 0.98–1.03). Conclusion Best evidence available to date does not support an independent association between coffee consumption and bladder cancer risk. Some direct associations observed in individual studies may be a result of residual confounding by smoking.
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- 2019
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28. Impaired dendritic cell functions in lung cancer: a review of recent advances and future perspectives
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Jing-Bo Wang, Xue Huang, and Fu-Rong Li
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Lung cancer ,Dendritic cell ,Immune regulation ,Immunotherapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Lung cancer is the leading cause of cancer mortality worldwide. Dendritic cells (DCs) are the key factors providing protective immunity against lung tumors and clinical trials have proven that DC function is reduced in lung cancer patients. It is evident that the immunoregulatory network may play a key role in the failure of the immune response to terminate tumors. Lung tumors likely employ numerous strategies to suppress DC-based anti-tumor immunity. Here, we summarize the recent advances in our understanding on lung tumor-induced immunosuppression in DCs, which affects the initiation and development of T-cell responses. We also describe which existing measures to restore DC function may be useful for clinical treatment of lung tumors. Furthering our knowledge of how lung cancer cells alter DC function to generate a tumor-supportive environment will be essential in order to guide the design of new immunotherapy strategies for clinical use.
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- 2019
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29. Derivation of soil Pb/Cd/As thresholds for safety of vegetable planting: A case study for pakchoi in Guangdong Province, China
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Fu-rong LI, Dian WEN, Fu-hua WANG, Fang-fang SUN, Xu WANG, Ying-qiong DU, Xiang-xiang LIU, and Kai WAN
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heavy metal pollution ,available content ,soil threshold ,correlation analysis ,vegetable ,Agriculture (General) ,S1-972 - Abstract
Regional guidelines for soil heavy metals, in conjunction with specific crops, are proposed to better assess soil environmental quality. In this study, large amounts of soils with different levels of Pb/Cd/As pollution were collected in Guangdong Province, China, to carry out a pot experiment, which was closer to the actual situation and properties of the heavy metal contaminated soils. Taking the widely planted vegetable pakchoi as the research object, we analyzed the correlations between the Pb/Cd/As concentrations in pakchoi and the total or available soil Pb/Cd/As concentrations and established their optimal regression equations. And then, the total and available soil Pb/Cd/As thresholds for pakchoi were calculated according to the allowable Pb/Cd/As limits in pakchoi and were compared with the current soil assessment standards. Furthermore, a lot of paired vegetable-soil samples in field were collected and their pollution situations were assessed by both the current limit thresholds and the calculated thresholds. Obviously, it was more consistent to assess the Cd and As pollution situation of the vegetable and soil samples with the calculated soil thresholds than the current soil assessment thresholds. It further proved that it was necessary to explore the soil heavy metal thresholds for safety of vegetable in specific regions, which would be more scientific and practical to guide safety production of local agricultural products and effective utilization of soil resources.
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- 2019
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30. The Effects of Farmyard Manure and Mulch on Soil Physical Properties in a Reclaimed Coastal Tidal Flat Salt-Affected Soil
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Jian-bing ZHANG, Jing-song YANG, Rong-jiang YAO, Shi-peng YU, Fu-rong LI, and Xiao-jing HOU
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farmyard manure ,mulch ,hydraulic property ,soil mechanical impedance ,tidal flat ,northern Jiangsu Province ,Agriculture (General) ,S1-972 - Abstract
Careful soil management is important for the soil quality and productivity improvement of the reclaimed coastal tidal flat saline land in northern Jiangsu Province, China. Farmyard manure (FYM) and mulch applications, which affect soil characteristics and plant significantly, are regard as an effective pattern of saline land improvement. As a conventional management in the study region, FYM and mulch are used for the amendment of the new reclaimed tidal flat regularly, but little is known about their effects on soil physical properties functioning. A study was conducted on a typical coastal tidal flat saline land, which was reclaimed in 2005, to evaluate the effects of FYM, polyethylene film mulch (PM), straw mulch (SM), FYM combined with PM (FYM+PM), FYM combined with SM (FYM+SM), on soil hydraulic properties and soil mechanical impedance. CK represented conventional cultivation in study area without FYM and mulch application and served as a control. The experiment, laid out in a randomized complete block design with three replications, was studied in Huanghaiyuan Farm, which specialized in the agricultural utilization for coastal tidal flat. Result showed that capillary water holding capacity (CHC), saturated water content (SWC), saturated hydraulic conductivity (Ks) and bulk density (BD), cone index (CI) were affected significantly by the FYM and mulch application, especially in the 0-10 cm soil layer. FYM and mulch management increased CHC, SWC and Ks over all soil depth in the order of FYM+SM>FYM+PM>FYM>SM>PM>CK. With the contrary sequence, BD and CI decreased significantly; however, FYM and mulch application affected BD and CI only in the upper soil layers. CHC, SWC and Ks decreased significantly with the increasing of soil depth, BD and CI, and a significant liner equation was found between CHC, SWC, Ks and BD, CI. With the highest CHC (38.15%), SWC (39.55%), Ks (6.00 mm h−1) and the lowest BD (1.26 g cm−3) and CI (2.71 MPa), the combined management of FYM and SM was recommend to be an effective method for the melioration of reclaimed coastal tidal flat saline soil.
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- 2014
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31. Preparation and Application of Conductive Polyaniline-Coated Thermally Expandable Microspheres
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Shou-Zheng Jiao, Zhi-Cheng Sun, Fu-Rong Li, Mei-Jia Yan, Mei-Juan Cao, Dong-Sheng Li, Yan Liu, and Lu-Hai Li
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thermally expandable microspheres ,suspension polymerization ,conductive polymer ,ink ,screen-printing ,Organic chemistry ,QD241-441 - Abstract
The thermally expandable microspheres (TEMs) were prepared through suspension polymerization with acrylonitrile (AN), methyl methacrylate (MMA) and methyl acrylate (MA) as the main monomers. Simultaneously, iso-pentane, n-hexane, iso-octane and other low-boiling hydrocarbons were prepared as blowing agents under two conditions, including high-pressure nitrogen and atmospheric conditions. The above physical foaming microspheres have a core-shell structure and excellent foaming effects. A layer of polyaniline (PANI) was deposited on the surface of the prepared TEMs by emulsion polymerization to obtain conductive and heat-expandable microspheres. Afterwards, the foaming ink was prepared by mixing the conductive TEMs and water-based ink. Finally, a conductive three-dimensional picture was obtained by screen-printing technology. This paper specifically focuses on the effects of particle size, morphology and the thermal expansion properties of the microspheres. The present research methods expect to obtain microspheres with a high foaming ratio, uniform particle size and antistatic properties, which may be applied to physical foaming ink.
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- 2018
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32. Dietary Fish and Long-Chain n-3 Polyunsaturated Fatty Acids Intake and Risk of Atrial Fibrillation: A Meta-Analysis
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Fu-Rong Li, Guo-Chong Chen, Jiabi Qin, and Xianbo Wu
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fish ,n-3 polyunsaturated fatty acids ,atrial fibrillation ,meta-analysis ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Findings on the association between long-term intake of fish or long-chain n-3 polyunsaturated fatty acids (PUFAs) and risk of atrial fibrillation (AF) are inconsistent in observational studies. We conducted a meta-analysis of prospective studies to separately examine the associations between fish consumption and dietary intake of n-3 PUFAs with the risk of AF. A systematic search was conducted in PubMed and Embase to identify relevant studies. Risk estimates were combined using a random-effect model. Seven prospective cohort studies covering 206,811 participants and 12,913 AF cases were eligible. The summary relative risk of AF for the highest vs. lowest category of fish consumption and dietary intake of n-3 PUFAs was 1.01(95% confidence interval: 0.94–1.09) and 1.03 (95% confidence interval: 0.97–1.09), respectively. These null associations persisted in subgroup and dose-response analyses. There was little evidence of publication bias. This meta-analysis suggests that neither long-term intake of fish, nor of n-3 PUFAs were significantly associated with lower risk of AF.
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- 2017
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33. The immunoregulation effect of alpha 1-antitrypsin prolong β-cell survival after transplantation.
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Yun Wang, Hong-Jie Yan, Shu-Yan Zhou, Yun-Shuang Wang, Hui Qi, Chun-Yan Deng, and Fu-Rong Li
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Medicine ,Science - Abstract
Islet transplantation has considerable potential as a cure for diabetes. However, the difficulties that arise from inflammation and the immunological rejection of transplants must be addressed for islet transplantation to be successful. Alpha 1-antitrypsin (AAT) inhibits the damage on β cells caused by inflammatory reactions and promotes β-cell survival and proliferation. This protein also induces specific immune tolerance to transplanted β cells. However, whether the expression of AAT in β cells themselves could eliminate or decrease immunological rejection of transplants is not clear. Therefore, we established a β cell line (NIT-hAAT) that stably expresses human AAT. Interestingly, in a cytotoxic T lymphocyte (CTL)-killing assay, we found that hAAT reduced apoptosis and inflammatory cytokine production in NIT-1 cells and regulated the Th1/Th2 cytokine balance in vitro. In vivo transplantation of NIT-hAAT cells into mice with diabetes showed hAAT inhibited immunological rejection for a short period of time and increased the survival of transplanted β cells. This study demonstrated that hAAT generated remarkable immunoprotective and immunoregulation effects in a model of β cell islet transplantation for diabetes model.
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- 2014
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34. Role of injured pancreatic extract promotes bone marrow-derived mesenchymal stem cells efficiently differentiate into insulin-producing cells.
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Hongbin Xie, Yunshuai Wang, Hui Zhang, Hui Qi, Hanxin Zhou, and Fu-Rong Li
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Medicine ,Science - Abstract
Mesenchymal stem cells (MSCs) can be successfully induced to differentiate into insulin-producing cells (IPCs) by a variety of small molecules and cytokines in vitro. However, problems remain, such as low transdifferentiation efficiency and poor maturity of trans-differentiated cells. The damaged pancreatic cells secreted a large amount of soluble proteins, which were able to promote pancreative islet regeneration and MSCs differentiation. In this study, we utilized the rat injured pancreatic tissue extract to modulate rat bone marrow-derived MSCs differentiation into IPCs by the traditional two-step induction. Our results showed that injured pancreatic tissue extract could effectively promote the trans-differentiation efficiency and maturity of IPCs by the traditional induction. Moreover, IPCs were able to release more insulin in a glucose-dependent manner and ameliorate better the diabetic conditions of streptozotocin (STZ)-treated rats. Our study provides a new strategy to induce an efficient and directional differentiation of MSCs into IPCs.
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- 2013
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35. Antifungal Activity of Eucalyptus Oil against Rice Blast Fungi and the Possible Mechanism of Gene Expression Pattern
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Li-Jun Zhou, Fu-Rong Li, Li-Jie Huang, Zhi-Rong Yang, Shu Yuan, and Lin-Han Bai
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E. grandis × E. urophylla oil ,antifungal effects ,M. grisea ,mRNA Genome Array ,biological control ,Organic chemistry ,QD241-441 - Abstract
Eucalyptus oil possesses a wide spectrum of biological activity, including anti-microbial, fungicidal, herbicidal, acaricidal and nematicidal properties. We studied anti-fungal activities of the leaf oil extracted from Eucalyptus. grandis × E. urophylla. Eleven plant pathogenic fungi were tested based on the mycelium growth rates with negative control. The results showed that Eucalyptus oil has broad-spectrum inhibitory effects toward these fungi. Remarkable morphological and structural alterations of hypha have been observed for Magnaporthe grisea after the treatment. The mRNA genome array of M. grisea was used to detect genes that were differentially expressed in the test strains treated by the Eucalyptus oil than the normal strains. The results showed 1919 genes were significantly affected, among which 1109 were down-regulated and 810 were up-regulated (p < 0.05, absolute fold change >2). According to gene ontology annotation analysis, these differentially expressed genes may cause abnormal structures and physiological function disorders, which may reduce the fungus growth. These results show the oil has potential for use in the biological control of plant disease as a green biopesticide.
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- 2016
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36. 3-(4-Bromophenyl)-1-(4-chlorobenzyl)-1H-pyrazole-5-carbaldehyde
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Fu-Rong Li, Yu-Juan Zhang, Feng-Guang Guo, Gui-Yun Duan, and Yan-Qing Ge
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Crystallography ,QD901-999 - Abstract
The title compound, C17H12BrClN2O, was synthesized by oxidation of [3-(4-bromophenyl)-1-(4-chlorobenzyl)-1H-pyrazol-5-yl]methanol under mild conditions. The pyrazole ring makes dihedral angles of 3.29 (9) and 74.91 (4)°, respectively, with the bromophenyl and chlorophenyl rings.
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- 2012
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37. Correction: Penicillamine Increases Free Copper and Enhances Oxidative Stress in the Brain of Toxic Milk Mice.
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Ding-Bang Chen, Li Feng, Xiao-Pu Lin, Wei Zhang, Fu-Rong Li, Xiu-Ling Liang, and Xun-Hua Li
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Medicine ,Science - Published
- 2012
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38. Penicillamine increases free copper and enhances oxidative stress in the brain of toxic milk mice.
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Ding-Bang Chen, Li Feng, Xiao-Pu Lin, Wei Zhang, Fu-Rong Li, Xiu-Ling Liang, and Xun-Hua Li
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Medicine ,Science - Abstract
Wilson disease (WD) is characterized by the accumulation of copper arising from a mutation in the ATP7B gene. Penicillamine (PA) makes 10-50% of the patients with neurologic symptoms neurologically worse at the early stage of administration. The aim of this study was to determine how the copper metabolism changes and whether the change impairs the brain of toxic milk (tx) mice, an animal model of WD, during the PA administration. The free copper and protein-bound copper concentrations in the serum, cortex and basal ganglia of tx mice with PA administration for 3 days, 10 days and 14 days, respectively, were investigated. The expression of copper transporters, ATP7A and CTR1,was analyzed by real-time quantitative PCR, immunofluorescence and Western blot. Then SOD, MDA and GSH/GSSG were detected to determine whether the oxidative stress changed correspondingly. The results revealed the elevated free copper concentrations in the serum and brain, and declined protein-bound copper concentrations in the brain of tx mice during PA administration. Meanwhile, transiently increased expression of ATP7A and CTR1 was observed generally in the brain parenchyma by immunofluorescence, real-time quantitative PCR and Western blot. Additionally, ATP7A and CTR1 were observed to locate mainly at Golgi apparatus and cellular membrane respectively. Intense staining of ATP7A in the choroid plexus was found in tx mice on the 3rd and 10th day of PA treatment, but rare staining of ATP7A and CTR1 in the blood-brain barrier (BBB). Decreased GSH/GSSG and increased MDA concentrations were also viewed in the cortex and basal ganglia. Our results suggested the elevated free copper concentrations in the brain might lead to the enhanced oxidative stress during PA administration. The increased free copper in the brain might come from the copper mobilized from brain parenchyma cells but not from the serum according to the ATP7A and CTR1 expression analysis.
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- 2012
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39. Microvascular disease, modifiable risk factor profiles and incident arrhythmias in type 2 diabetes.
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Guo-Chong Chen, Hukportie, Daniel Nyarko, Wei-Dong Fan, Jie-Qiong Lyu, Hai-Peng Wang, Liqiang Qin, Xian-Bo Wu, and Fu-Rong Li
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ARRHYTHMIA ,TYPE 2 diabetes ,VENTRICULAR arrhythmia - Published
- 2024
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40. Waist Circumference and Body Mass Index Variability and Incident Diabetic Microvascular Complications: A Post Hoc Analysis of ACCORD Trial
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Daniel Nyarko Hukportie, Fu-Rong Li, Rui Zhou, Jia-Zhen Zheng, Xiao-Xiang Wu, and Xian-Bo Wu
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Diabetes Mellitus, Type 2 ,Retinal Diseases ,Heart Disease Risk Factors ,Risk Factors ,Endocrinology, Diabetes and Metabolism ,Humans ,Obesity ,Waist Circumference ,Body Mass Index - Abstract
Background: Obesity is associated with adverse health events among diabetic patients, however, the relationship between obesity fluctuation and risk of microvascular complications among this specific population is unclear. We aimed to examine the effect of waist circumference (WC) and body mass index (BMI) variability on the risk of diabetic microvascular outcomeMethods: Annually recorded anthropometric data in the Action to Control Cardiovascular Risk in Diabetes (ACCORD) study was used to examine the association of WC and BMI variability defined as variability independent of mean, with the risk of microvascular outcomes, including neuropathy, nephropathy, and retinopathy. Cox proportional hazards models were used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) (Trial registration: ClinicalTrials.gov., no. NCT00000620).Results: There were 4,031, 5,369, and 2,601 cases of neuropathy, nephropathy, and retinopathy during a follow-up period of 22,524, 23,941, and 23,850 person-years, respectively. Higher levels of WC and BMI variability were associated with an increased risk of neuropathy. Compared with the lowest quartile, the fully-adjusted HR (95% CI) for the highest quartile of WC and BMI variability for neuropathy risk were 1.21 (1.05 to 1.40) and 1.16 (1.00 to 1.33), respectively. Also, higher quartiles of BMI variability but not WC variability were associated with increased risk of nephropathic events. The fully-adjusted HR (95% CI) for the highest quartile compared with the lowest quartile of BMI variability was 1.31 (1.18 to 1.46). However, the results for retinopathic events were all insignificant.Conclusion: Among participants with type 2 diabetes mellitus, WC and BMI variability were associated with a higher risk of neuropathic events, whereas BMI variability was associated with an increased risk of nephropathic events.
- Published
- 2022
41. Multimorbidity and mortality among older patients with coronary heart disease in Shenzhen, China.
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Fu-Rong LI, Shuang WANG, Xia LI, Zhi-Yuan CHENG, Cheng JIN, Chun-Bao MO, Jing ZHENG, Feng-Chao LIANG, and Dong-Feng GU
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CARDIOVASCULAR disease related mortality ,RISK assessment ,CORONARY disease ,RESEARCH funding ,KRUSKAL-Wallis Test ,RETROSPECTIVE studies ,HEART failure ,DESCRIPTIVE statistics ,CHI-squared test ,MANN Whitney U Test ,LONGITUDINAL method ,MEDICAL records ,ACQUISITION of data ,ISCHEMIC stroke ,HOSPITAL care of older people ,TUMORS ,CONFIDENCE intervals ,DATA analysis software ,COMORBIDITY ,PROPORTIONAL hazards models ,OLD age - Abstract
BACKGROUND The current understanding of the magnitude and consequences of multimorbidity in Chinese older adults with coronary heart disease (CHD) is insufficient. We aimed to assess the association and population-attributable fractions (PAFs) between multimorbidity and mortality among hospitalized older patients who were diagnosed with CHD in Shenzhen, China. METHODS We conducted a retrospective cohort study of older Chinese patients (aged = 65 years) who were diagnosed with CHD. Cox proportional hazards models were used to estimate the associations between multimorbidity and all-cause and cardiovascular disease (CVD) mortality. We also calculated the PAFs. RESULTS The study comprised 76,455 older hospitalized patients who were diagnosed with CHD between January 1, 2016, and August 31, 2022. Among them, 70,217 (91.9%) had multimorbidity, defined as the presence of at least one of the predefined 14 chronic conditions. Those with cancer, hemorrhagic stroke and chronic liver disease had the worst overall death risk, with adjusted HRs (95% CIs) of 4.05 (3.77, 4.38), 2.22 (1.94, 2.53), and 1.85 (1.63, 2.11), respectively. For CVD mortality, the highest risk was observed for hemorrhagic stroke, ischemic stroke, and chronic kidney disease; the corresponding adjusted HRs (95% CIs) were 3.24 (2.77, 3.79), 1.91 (1.79, 2.04), and 1.81 (1.64, 1.99), respectively. All-cause mortality was mostly attributable to cancer, heart failure and ischemic stroke, with PAFs of 11.8, 10.2, and 9.1, respectively. As for CVD mortality, the leading PAFs were heart failure, ischemic stroke and diabetes; the corresponding PAFs were 18.0, 15.7, and 6.1, respectively. CONCLUSIONS Multimorbidity was common and had a significant impact on mortality among older patients with CHD in Shenzhen, China. Cancer, heart failure, ischemic stroke and diabetes are the primary contributors to PAFs. Therefore, prioritizing improved treatment and management of these comorbidities is essential for the survival prognosis of CHD patients from a holistic public health perspective. [ABSTRACT FROM AUTHOR]
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- 2024
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42. Glomerular filtration rate estimated by differing measures and risk of all‐cause mortality among Chinese individuals without or with diabetes: A nationwide prospective study
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Yu‐Jie Liu, Fu‐Rong Li, Cai‐Long Chen, Zhong‐Xiao Wan, Jin‐Si Chen, Jing Yang, Rong Liu, Jia‐Ying Xu, Yan Zheng, Li‐Qiang Qin, and Guo‐Chong Chen
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Endocrinology, Diabetes and Metabolism - Published
- 2023
43. The association between daytime napping and risk of type 2 diabetes is modulated by inflammation and adiposity: Evidence from 435 342 <scp>UK‐Biobank</scp> participants
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Rui Zhou, Hao‐Wen Chen, Yi‐Ning Huang, Qi Zhong, Fu‐Rong Li, Rui‐Dian Huang, Hua‐Min Liu, Jia‐Zhen Zheng, Jia‐Wen Xu, and Xian‐Bo Wu
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Endocrinology, Diabetes and Metabolism - Published
- 2023
44. Association of Lifestyle With Incidence of Heart Failure According to Metabolic and Genetic Risk Status: A Population-Based Prospective Study
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Zhengbao Zhu, Fu-Rong Li, Yiming Jia, Yang Li, Daoxia Guo, Jingsi Chen, Haili Tian, Jing Yang, Huan-Huan Yang, Li-Hua Chen, Kaixin Zhang, Pinni Yang, Lulu Sun, Mengyao Shi, Yonghong Zhang, Li-Qiang Qin, and Guo-Chong Chen
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Heart Failure ,Cholesterol ,Risk Factors ,Incidence ,Humans ,Prospective Studies ,Cardiology and Cardiovascular Medicine ,Life Style - Abstract
Background: Whether lifestyle factors are similarly associated with risk of heart failure (HF) for individuals with different metabolic or genetic risk status remains unclear. Methods: We included 464 483 participants from UK Biobank who were free of major cardiovascular disease or HF during baseline recruitment. Healthy lifestyle factors included avoidance of smoking, no obesity, regular physical activity, and healthy diet. Lifestyle was categorized as favorable (3 or 4 healthy lifestyle factors), intermediate (2 healthy lifestyle factors), and unfavorable (0 or 1 healthy lifestyle factor) lifestyles. Metabolic status was defined by the presence of hypertension, high total cholesterol, or diabetes at baseline. A weighted genetic risk score was created based on 12 single-nucleotide polymorphisms associated with HF. Results: Compared with favorable lifestyle, the multivariable-adjusted hazard ratios of HF were 1.79 (95% CI, 1.68–1.90) and 2.90 (95% CI, 2.70–3.11) for intermediate lifestyle and unfavorable lifestyle, respectively ( P trend P interaction ≥0.21). The association was also similar across different genetic risk categories ( P interaction =0.92). In a joint analysis, the hazard ratio of HF was 4.05 (95% CI, 3.43–4.77) comparing participants who had both higher genetic risk and an unfavorable lifestyle with those having lower genetic risk and a favorable lifestyle. Conclusions: Combined lifestyle was associated with incident HF regardless of metabolic or genetic risk status, supporting the recommendation of healthy lifestyles for HF prevention across the entire population.
- Published
- 2022
45. hucMSC-derived exosomes ameliorate pressure ulcers by inhibiting HMGB1
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Fei Yan, Meihua Gong, Fu-Rong Li, and Li Yu
- Abstract
Background:Pressure ulcers (PUs) are a type of chronic wound in the elderly population. Previous studies have shown that exosomes derived from stem cells contain cytokines and growth factors that affect tissue repair and can represent a therapeutic strategy for wound healing. Thus, fully understanding how to extract exosomes and their mechanism of action can help promote the management of chronic refractory wound healing as a new cell-free treatment model. Methods:In this study, we isolated exosomes from human umbilical cord mesenchymal stem cells (hucMSC-Exos) and examined their effects on wound healing. A total of 15 mice that were randomly divided into three groups, subjected to three ischemia–reperfusion (I-R) cycles and treated with different doses of hucMSC-Exos for different times. Quantitative real-time polymerase chain reaction (qRT–PCR) was used to analyze collagen mRNA levels in tissue samples. HMGB1 levels were examined by Western blotting and immunohistochemistry. α-SMA, CD34, and HMGB1 expression levels were compared to investigate the potential mechanisms. Results:We found that hucMSC-Exos could be taken up by fibroblasts and significantly regulated and improved fibroblast fibrosis and in PU wound healing in vivo. Furthermore, we observed that hucMSC-Exo treatment of PU wounds downregulated the expression of HMGB1, which was previously shown to have a deleterious effect on the wound healing process. Conclusions:Our findings indicate that hucMSC-Exos regulate the repair of PU wounds in part by inhibiting HMGB1 expression . Exosome treatment has provided new perspectives in regenerative medicine and trauma management.
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- 2022
46. Long-term exposure to air pollution and incident non-alcoholic fatty liver disease and cirrhosis: A cohort study
- Author
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Fu‐Rong Li, Jian Liao, Bin Zhu, Xia Li, Zhiyuan Cheng, Cheng Jin, Chunbao Mo, Xianbo Wu, Qian Li, and Fengchao Liang
- Subjects
Hepatology - Abstract
Epidemiological evidence regarding the association of air pollution with the risk of non-alcoholic fatty liver disease (NAFLD) is limited. This study was to examine the associations of long-term exposure to various air pollutants and overall air pollution with risk of incident NAFLD as well as cirrhosis, a major liver-related morbidity for NAFLD.Included were 456 687 UK residents. Air pollution data included PMWe identified 4978 cases of NAFLD and 1575 cases of incident cirrhosis, over a median follow-up of 11.9 years. PMLong-term exposure to air pollution was associated with risks of NAFLD and cirrhosis among the UK population.
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- 2022
47. Microvascular burden and incident heart failure among middle-aged and older adults with type 1 or type 2 diabetes
- Author
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Fu-Rong Li, Daniel Nyarko Hukportie, Jing Yang, Huan-Huan Yang, Guo-Chong Chen, and Xian-Bo Wu
- Subjects
Heart Failure ,Advanced and Specialized Nursing ,Diabetes Mellitus, Type 1 ,Diabetes Mellitus, Type 2 ,Retinal Diseases ,Risk Factors ,Incidence ,Endocrinology, Diabetes and Metabolism ,Internal Medicine ,Humans ,Middle Aged ,Renal Insufficiency, Chronic ,Aged - Abstract
OBJECTIVE To examine the association between microvascular disease (MVD) and risk of heart failure (HF) among individuals with type 1 diabetes mellitus (T1DM) or type 2 diabetes mellitus (T2DM). RESEARCH DESIGN AND METHODS We included 1,713 and 28,624 participants with T1DM and T2DM, respectively, from the UK Biobank who were free of HF during enrollment. MVD burden reflected by the presence of retinopathy, peripheral neuropathy, and chronic kidney disease (CKD) at baseline was prospectively evaluated for the association with incidence of HF. Hazard ratios (HRs) and 95% CIs of HF were estimated by Cox regression models adjusted for multiple traditional risk factors. RESULTS There were 145 and 2,515 incident cases of HF recorded among participants with T1DM and T2DM, respectively, during a median follow-up of 11.5 years. The association between the number of MVD and HF was stronger among participants with T1DM than among those with T2DM (P for interaction CONCLUSIONS While a heavier burden of MVD was associated with excess risk of HF both in T1DM and T2DM, the association was evidently more pronounced in T1DM.
- Published
- 2022
48. Influence of Diabetes Duration and Glycemic Control on Dementia: A Cohort Study
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Hai-Lian Yang, Rui Zhou, Guo-Chong Chen, Xian-Bo Wu, Qiang Fu, Fu-Rong Li, Jiazhen Zheng, Jing-Ya Wang, Xiao-Xiang Wu, and Meng-Chen Zou
- Subjects
Blood Glucose ,Aging ,medicine.medical_specialty ,Glycemic Control ,Cohort Studies ,chemistry.chemical_compound ,Risk Factors ,Internal medicine ,Diabetes mellitus ,Diabetes Mellitus ,medicine ,Humans ,Dementia ,Prospective Studies ,Prospective cohort study ,Glycemic ,Glycated Hemoglobin ,business.industry ,Hazard ratio ,medicine.disease ,Diabetes Mellitus, Type 2 ,chemistry ,Glycated hemoglobin ,Geriatrics and Gerontology ,Risk assessment ,business ,Cohort study - Abstract
Background To investigate the influence of diabetes duration and glycemic control, assessed by glycated hemoglobin (HbA1c) levels, on risk of incident dementia. Methods The present study is a prospective study of 461 563 participants from the UK Biobank. The age at diabetes diagnosis was determined by self-report. Diabetes duration was calculated as baseline age minus age at diagnosis. Cox proportional hazards regression models were used to estimate hazard ratios (HRs) with 95% confidential intervals (CIs). Results During a median follow-up of 8.1 years, 2 233 dementia cases were recorded. As compared with normoglycemic individuals, individuals with diabetes had higher risk of all-cause dementia, and the risk increased with increasing duration of diabetes; compared with participants with diabetes duration of Conclusions Diabetes duration appeared to be associated with the risk of incident dementia due to factors beyond glycemic control. Clinicians should consider not only glycemic control but also diabetes duration in dementia risk assessments for patients with diabetes.
- Published
- 2021
49. The combination therapy with EpCAM/CD3 BsAb and MUC-1/CD3 BsAb elicited antitumor immunity by T-cell adoptive immunotherapy in lung cancer
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Yingjuan Wu, Di Wu, Ce Wang, Shang Chen, Jingbo Wang, and Fu-Rong Li
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Lung Neoplasms ,Combination therapy ,CD3 Complex ,T cell ,medicine.medical_treatment ,CD3 ,T-Lymphocytes ,Population ,Immunotherapy, Adoptive ,Antineoplastic Agents, Immunological ,Antigen ,Cell Line, Tumor ,medicine ,Humans ,Lung cancer ,education ,non-small cell lung cancer ,MUC-1/CD3 BsAb ,education.field_of_study ,biology ,business.industry ,Mucin-1 ,General Medicine ,Immunotherapy ,medicine.disease ,Epithelial Cell Adhesion Molecule ,CTL ,medicine.anatomical_structure ,biology.protein ,Cancer research ,EpCAM/CD3 BsAb ,immunotherapy ,business ,Research Paper - Abstract
Lung cancer remains a global challenge due to high morbidity and mortality rates and poor response to treatment, and there are still no effective strategies to solve it. The bispecific antibody (BsAb) is a novel antibody, which can target two different antigens and mediate specific killing effects by selectively redirecting effector cells to the target cells. In this study, we combined two BsAbs to achieve a dual-target therapy strategy of EpCAM+ and MUC-1+ with high affinity and specificity. The results showed that the combination of two BsAbs against EpCAM and MUC-1 could inhibit the growth of lung cancer more effectively in cell lines and primary tumors. The superior antitumor effect of two BsAbs could be attributable to enhanced CTL and increased production of type I IFNs. At the same time, the combination of EpCAM/CD3 BsAb and MUC-1/CD3 BsAb significantly regulated T population in the TDLNs. Therefore, we have found a potential immunotherapeutic strategy, which was the combination therapy with EpCAM/CD3 BsAb and MUC-1/CD3 BsAb for the treatment of non-small cell lung cancer.
- Published
- 2021
50. Early neurological deterioration during the acute phase as a predictor of long-term outcome after first-ever ischemic stroke
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Geng, He-Hong, Wang, Qiang, Li, Bo, Cui, Bin-Bin, Jin, Yong-Ping, Fu, Rong-Li, Zhang, Qing, Wang, Jing-Jie, and Wang, Pei-Xi
- Published
- 2017
- Full Text
- View/download PDF
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