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159 results on '"Fu, Yajuan"'

9. RACK1 links phyB and BES1 to coordinate brassinosteroid‐dependent root meristem development.

Author

Zhu, Wei, Fu, Yajuan, Zhou, Hua, Zhou, Yeling, Zhang, Dayan, Wang, Yuzhu, Su, Yujing, Li, Zhiyong, and Liang, Jiansheng

Abstract

Summary Light and brassinosteroids (BR) are indispensable for plant growth and control cell division in the apical meristem. However, how external light signals cooperate with internal brassinosteroids to program root meristem development remains elusive. We reveal that the photoreceptor phytochrome B (phyB) guides the scaffold protein RACK1 to coordinate BR signaling for maintaining root meristematic activity. phyB and RACK1 promote early root meristem development. Mechanistically, RACK1 could reinforce the phyB–SPA1 association by interacting with both phyB and SPA1, which indirectly affects COP1‐dependent RACK1 degradation, resulting in the accumulation of RACK1 in roots. Subsequently, RACK1 interacts with BES1 to repress its DNA‐binding activity toward the target gene CYCD3;1, leading to the release of BES1‐mediated inhibition of CYCD3;1 transcription, and hence the promotion of root meristem development. Our study provides mechanistic insights into the regulation of root meristem development by combination of light and phytohormones signals through the photoreceptors and scaffold proteins. [ABSTRACT FROM AUTHOR]

Published
2024
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21. Scaffold protein RACK1 regulates BR signaling by modulating the nuclear localization of BZR1.

Author

Li, Zhiyong, Fu, Yajuan, Wang, Yuzhu, and Liang, Jiansheng

Subjects
*SCAFFOLD proteins, *NUCLEOCYTOPLASMIC interactions, *BRASSINOSTEROIDS, *PROTEIN receptors, *STEROID hormones, *CYTOSOL
Abstract

Summary: Brassinosteroids (BRs) are a group of plant‐specific steroid hormones, which induces the rapid nuclear localization of the positive transcriptional factors BRASSINAZOLE RESISTANT1/2 (BZR1/2). However, the mechanisms underlying the regulation of nucleocytoplasmic shuttling of BZR1 remain to be fully elucidated.In this study, we show that the scaffold protein Receptor for Activated C Kinase 1 (RACK1) from Arabidopsis is involved in BR signaling cascades through mediating the nuclear localization of BZR1, which is tightly retained in the cytosol by the conserved scaffold protein 14‐3‐3s.RACK1 can interact with BZR1 and competitively decrease the 14‐3‐3 interaction with BZR1 in cytosol, which efficiently enhances the nuclear localization of BZR1. 14‐3‐3 also retains RACK1 in cytosol through their interaction. Conversely, BR treatment enhances the nuclear localization of BZR1 by disrupting the 14‐3‐3 interaction with RACK1 and BZR1.Our study uncovers a new mechanism that integrates two kinds of conserved scaffold proteins (RACK1 and 14‐3‐3) coordinating BR signaling event. [ABSTRACT FROM AUTHOR]

Published
2023
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26. Identification and molecular characterization of the alternative spliced variants of beta carbonic anhydrase 1 (βCA1) from Arabidopsis thaliana

Author

Shen, Jinyu, Li, Zhiyong, Fu, Yajuan, and Liang, Jiansheng

Subjects
Carbonic anhydrase, Protein-protein interaction network, General Neuroscience, Stress response, Plant Science, General Medicine, Spliced variant, General Biochemistry, Genetics and Molecular Biology, Localization, Medicine, Expression pattern, General Agricultural and Biological Sciences, Molecular Biology
Abstract

Carbonic anhydrases (CAs) are ubiquitous zinc metalloenzymes that catalyze the interconversion of carbon dioxide and bicarbonate. Higher plants mainly contain the three evolutionarily distinct CA families αCA, βCA, and γCA, with each represented by multiple isoforms. Alternative splicing (AS) of the CA transcripts is common. However, there is little information on the spliced variants of individual CA isoforms. In this study, we focused on the characterization of spliced variants of βCA1 from Arabidopsis. The expression patterns and subcellular localization of the individual spliced variants of βCA1 were examined. The results showed that the spliced variants of βCA1 possessed different subcellular and tissue distributions and responded differently to environmental stimuli. Additionally, we addressed the physiological role of βCA1 in heat stress response and its protein-protein interaction (PPI) network. Our results showed that βCA1 was regulated by heat stresses, and βca1 mutant was hypersensitive to heat stress, indicating a role for βCA1 in heat stress response. Furthermore, PPI network analysis revealed that βCA1 interacts with multiple proteins involved in several processes, including photosynthesis, metabolism, and the stress response, and these will provide new avenues for future investigations of βCA1.

Published
2021

27. Metabolome and Whole-Transcriptome Analyses Reveal the Molecular Mechanisms Underlying Hypoglycemic Nutrient Metabolites Biosynthesis in Cyclocarya paliurus Leaves During Different Harvest Stages

Author

Zheng, Xuehai, primary, Xiao, Huibao, additional, Chen, Jiannan, additional, Zhu, Jinmao, additional, Fu, Yajuan, additional, Ouyang, Songying, additional, Chen, Youqiang, additional, Chen, Duo, additional, Su, Jingqian, additional, and Xue, Ting, additional

Published
2022
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36. Zebularine elevates STING expression and enhances cGAMP cancer immunotherapy in mice

Author

Lai, Junzhong, primary, Fu, Yajuan, additional, Tian, Shuoran, additional, Huang, Shanlu, additional, Luo, Xuan, additional, Lin, Lili, additional, Zhang, Xing, additional, Wang, Hanze, additional, Lin, Zhang, additional, Zhao, Heng, additional, Lin, Shujin, additional, Zhao, Junhong, additional, Xu, Shan, additional, Li, Daliang, additional, Cai, Shaoli, additional, Dong, Luna, additional, Qian, Jing, additional, Liang, Jiadi, additional, Li, Qiumei, additional, Zhang, Yong, additional, Fan, Jiqiang, additional, Balderas, Robert, additional, and Chen, Qi, additional

Published
2021
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39. Insights into algae evolution for adapting to blue-green light garnered from the Isochrysis galbana genome

Author

Chen, Duo, primary, Yuan, Xue, additional, Zheng, Xuehai, additional, Fang, Jingping, additional, Lin, Gang, additional, Chen, Jiannan, additional, Li, Daliang, additional, Li, Li, additional, He, Wejin, additional, Huang, Luqiang, additional, Huang, Zhen, additional, Li, Rongmao, additional, Fu, Yajuan, additional, Fan, Wenfang, additional, Liang, Limin, additional, Chen, Nan, additional, Lin, Chentao, additional, Zhu, Jinmao, additional, Chen, Youqiang, additional, and Xue, Ting, additional

Published
2020
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42. Identification and analysis of differentially expressed long non-coding RNAs of Chinese Holstein cattle responses to heat stress

Author

Xiaolan Shang, Zhuodong Chu, Mingxing Chu, Zhengfeng Zhang, Qiuling Li, Jie Qiao, and Fu Yajuan

Subjects
0301 basic medicine, Bioengineering, Computational biology, Biology, Genome, 03 medical and health sciences, 0302 clinical medicine, Mammary Glands, Animal, microRNA, Transcriptional regulation, Animals, Gene, Dairy cattle, Sequence Analysis, RNA, RNA, High-Throughput Nucleotide Sequencing, Long non-coding RNA, Dairying, 030104 developmental biology, Gene Expression Regulation, 030220 oncology & carcinogenesis, Animal Science and Zoology, Cattle, Female, RNA, Long Noncoding, Function (biology), Heat-Shock Response, Biotechnology
Abstract

The mechanisms that dairy cattle respond to environmental stresses are very complicated. Previous research into the molecular mechanisms of mammalian heat stress has largely focused on the role of protein-coding genes and small non-coding RNAs. Recently, it has become apparent that large numbers of long non-coding RNAs transcribed from mammalian genomes play extensive roles in transcriptional regulation. However, the expression of lncRNAs and their biological functions in heat stress in dairy cattle remain unknown. In this study, we employed a deep RNA sequencing to examine lncRNA expression profiles of heat stressed and non-heat stressed Chinese Holstein cattle. We found that 24,795 novel and 3763 known lncRNAs were expressed in the bovine mammary gland, of which 174 were differentially expressed in heat stress condition, among them, 156 lncRNAs were up-regulated and 18 were down-regulated. Through Cis role analysis, 16,474 lncRNAs were transcribed close to protein-coding neighbors. In addition, 11 and 2024 lncRNAs harbored precursors of known and predicted microRNAs, respectively, were annotated in the precursor analysis of miRNAs. Taken together, our findings represent the first systematic investigation of lncRNA expression in heat stressed Chinese Holstein and provide a resource for further research into the molecular mechanisms of lncRNAs function in dairy cattle.

Published
2018

47. Identification and analysis of differentially expressed long non-coding RNAs of Chinese Holstein cattle responses to heat stress.

Author

Li, Qiuling, Qiao, Jie, Zhang, Zhengfeng, Shang, Xiaolan, Chu, Zhuodong, Fu, Yajuan, and Chu, Mingxing

Subjects
HOLSTEIN-Friesian cattle, NON-coding RNA, DAIRY cattle, ENTHALPY, NUCLEOTIDE sequence, BOS, CATTLE handling
Abstract

The mechanisms that dairy cattle respond to environmental stresses are very complicated. Previous research into the molecular mechanisms of mammalian heat stress has largely focused on the role of protein-coding genes and small non–coding RNAs. Recently, it has become apparent that large numbers of long non-coding RNAs transcribed from mammalian genomes play extensive roles in transcriptional regulation. However, the expression of lncRNAs and their biological functions in heat stress in dairy cattle remain unknown. In this study, we employed a deep RNA sequencing to examine lncRNA expression profiles of heat stressed and non-heat stressed Chinese Holstein cattle. We found that 24,795 novel and 3763 known lncRNAs were expressed in the bovine mammary gland, of which 174 were differentially expressed in heat stress condition, among them, 156 lncRNAs were up-regulated and 18 were down-regulated. Through Cis role analysis, 16,474 lncRNAs were transcribed close to protein-coding neighbors. In addition, 11 and 2024 lncRNAs harbored precursors of known and predicted microRNAs, respectively, were annotated in the precursor analysis of miRNAs. Taken together, our findings represent the first systematic investigation of lncRNA expression in heat stressed Chinese Holstein and provide a resource for further research into the molecular mechanisms of lncRNAs function in dairy cattle. [ABSTRACT FROM AUTHOR]

Published
2020
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