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24 results on '"Fu, Weilai"'

8. Urotensin II Enhances Advanced Aortic Atherosclerosis Formation and Delays Plaque Regression in Hyperlipidemic Rabbits

Author

Yu, Qingqing, primary, Wei, Panpan, additional, Xu, Liran, additional, Xia, Congcong, additional, Li, Yafeng, additional, Liu, Haole, additional, Song, Xiaojie, additional, Tian, Kangli, additional, Fu, Weilai, additional, Wang, Rong, additional, Wang, Weirong, additional, Bai, Liang, additional, Fan, Jianglin, additional, Liu, Enqi, additional, and Zhao, Sihai, additional

Published
2023
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10. Dapagliflozin Ameliorates the Formation and Progression of Experimental Abdominal Aortic Aneurysms by Reducing Aortic Inflammation in Mice

Author

Liu, Haole, primary, Wei, Panpan, additional, Fu, Weilai, additional, Xia, Congcong, additional, Li, Yankui, additional, Tian, Kangli, additional, Li, Yafeng, additional, Cheng, Daxin, additional, Sun, Jiaying, additional, Xu, Yangwei, additional, Lu, Ming, additional, Xu, Boyu, additional, Zhang, Yali, additional, Wang, Rong, additional, Wang, Weirong, additional, Xu, Baohui, additional, Liu, Enqi, additional, and Zhao, Sihai, additional

Published
2022
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13. Isolation and Identification of an Efficient Aerobic Denitrifying Pseudomonas stutzeri Strain and Characterization of Its Nitrite Degradation

Author

Chao Liu, Xian Zhang, Fu Weilai, Wang Yunshuang, Qiang Wang, Shuhui Chen, Wang Yaru, Zhiming Rao, Yi Ganfeng, and Peifeng Duan

Subjects
biology, Strain (chemistry), Chemistry, Chemical technology, chemistry.chemical_element, TP1-1185, biology.organism_classification, Nitrogen, Catalysis, Pseudomonas stutzeri, chemistry.chemical_compound, Denitrifying bacteria, domestication, Nutrient pollution, nitrogen pollution, Food science, Physical and Theoretical Chemistry, Nitrite, Eutrophication, nitrite removal, QD1-999, Bacteria
Abstract

Nitrogen pollution in water bodies is becoming increasingly serious, and how to remove nitrogen from water bodies economically and effectively has become a research hotspot. Especially in recent years, with the gradual expansion of aquaculture in China, the content of nitrite and other nitrogen-containing substances in water bodies has been increasing, which inhibits the growth of farm animals and is one of the causes of eutrophication in water bodies. In this study, a strain of bacteria was isolated from the sludge of an aquaculture fishpond and identified as Pseudomonas stutzeri, which can efficiently degrade nitrite. After continuous domestication in nitrite mixed solution, the nitrite nitrogen reduction capacity of P. stutzeri was significantly improved. Univariate experiments aiming to optimize the degradation conditions indicate that the optimal culture conditions for strain F2 are: medium with a carbon source of sodium succinate, C/N of 18, pH of 8, culture temperature of 28 °C, and shaking speed of 210 rpm in the shaker. Under the optimal culture conditions, the NO2−-N concentration of the culture solution was 300 mg/L, and the nitrite removal rate reached 98.67%. Meanwhile, the results of the nitrogen balance test showed that the strain converted 6.1% of the initial nitrogen into cellular organic nitrogen and 62.3% into gaseous nitrogen.

Published
2021

22. Characterization of Bacillus subtilisAb03 for efficient ammonia nitrogen removal

Author

Wang, Qiang, Fu, Weilai, Lu, Ruiqi, Pan, Chunli, Yi, Ganfeng, Zhang, Xian, and Rao, Zhiming

Abstract

An efficient ammonia nitrogen degrading bacterial strain was isolated from a fish and shrimp pond and identified as Bacillus subtilis(Ab03). Firstly, the strain was continuously domesticated in ammonium solution to improve its nitrogen removal capacity. The performance of the strain in terms of nitrogen removal efficiency under different culture conditions was then examined. Finally, the nitrogen removal process and related strain mechanisms were analyzed by nitrogen balance. The results showed the strain Ab03 could remove 91.67% of NH4+-N at 300 mg/L under the conditions of glucose as the single carbon source, C/N of 15, pH of 7.5, the temperature of 35 ℃ and dissolved oxygen of 7–8 mg/L. It was also found that under conditions where ammonia nitrogen was the only nitrogen source, strain Ab03 could also undergo aerobic denitrification for simultaneous conversion, with a final gas conversion rate of 74.81%.

Published
2022
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24. Effect of Pseudomonas stutzeriF2 on rearing water quality and growth, innate immunity, visceral morphology and gut microbiota structure of juvenile spotted seabass (Lateolabrax maculatus)

Author

Fu, Weilai, Duan, Peifeng, Wang, Qiang, Song, Junying, Wang, Yunshuang, Zhang, Zhen, Wang, Pan, Jiang, Huihui, Zhang, Xian, Song, Guolong, and Rao, Zhiming

Abstract

Previous reports on the effects of probiotics on farmed fish have focused more on dietary supplementation, and this study evaluated the effects of Pseudomonas stutzeriF2 supplementation in rearing water on juvenile spotted seabass (Lateolabrax maculatus). Different from the control group (T0), where ammonia nitrogen levels were reduced by water exchange in the circulation system, the T1 and T2 groups supplemented the rearing water with low (3.0 × 103CFU/mL) and high (3.0 × 105CFU/mL) concentrations of P. stutzeriF2 with the ability to remove ammonia nitrogen and nitrite, respectively. After 33 d of rearing, the weight gain rate, specific growth rate, feed utilization and survival rate of the experimental group of spotted seabass were significantly improved (p< 0.05). P. stutzeriF2 activated superoxide dismutase (SOD), lysozyme (LZM) and catalase (CAT) activities in the serum of the experimental group of spotted seabass; increased glutathione peroxidase (GSH-Px) activity in the liver while decreasing malondialdehyde (MDA) levels. Microscopic observation of tissue sections revealed significantly improved muscle thickness and mucosal fold height in the T2 group of the midgut. The study of changes in the gut microbiota of spotted seabass by 16S rRNA gene sequencing revealed a significant decline in the relative abundance of Proteobacteria (phylum) and Photobacterium(genus) in the P. stutzeriF2 supplemented groups. Aeromonas veroniichallenge test to assess the disease resistance of spotted seabass found that the survival rate of the T2 group was the highest. Our study showed that the supplementation of P. stutzeriF2 with 3.0 × 105CFU/mL in the rearing water increased the growth performance of spotted seabass, activated innate immunity, improved the relative abundance of gut microbiota, and enhanced resistance to A. veronii, providing a new idea for the application of probiotics.

Published
2023
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