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11 results on '"Fu, Chuan-Yang"'

4. Determination of picroside II in dog plasma by HPLC and its application in a pharmacokinetics study

Author

Fu-chuan Yang, Shi-Lin Yang, and Li-Zhen Xu

Subjects
Male, Correlation coefficient, Calibration curve, Iridoid Glucosides, Clinical Biochemistry, Analytical chemistry, Sensitivity and Specificity, Biochemistry, High-performance liquid chromatography, Analytical Chemistry, chemistry.chemical_compound, Dogs, Drug Stability, Glucosides, Pharmacokinetics, Spectrophotometry, Freezing, Drug Discovery, medicine, Animals, Molecular Biology, Chromatography, High Pressure Liquid, Pharmacology, Detection limit, Chromatography, medicine.diagnostic_test, Chemistry, Reproducibility of Results, General Medicine, Plasma, Paeoniflorin, Cinnamates, Spectrophotometry, Ultraviolet
Abstract

A sensitive and simple high-performance liquid chromatography method with UV detection was developed and validated for determining picroside II in dog plasma. Paeoniflorin was employed as internal standard and the sample pre-treatment procedure consists of deproteinization by addition of acetonitrile. Chromatographic separations were performed on a Shimadzu VP-ODS column (250 x 4.6 mm i.d., 5 microm). The mobile phase consisted of acetonitrile-0.1% acetic acid aqueous (v/v), 23:77, v/v, at a rate of 1 mL/min. Detection was carried out at a wavelength of 266 nm. Calibration standards ranged from 0.25 to 500 microg/mL in dog plasma and the mean correlation coefficient of 0.9981 was found for the linear calibration curves (n = 6). The limit of quantification (LOQ) was 0.25 microg/mL. Intra- and inter-assay RSD ranged from 0.70 to 7.5%. Accuracy (%bias) ranged from -6.3 to 6.0%. This method was applied to the pharmacokinetic study of picroside II in dogs. The study demonstrated the plasma picroside II concentration-time curves were fitted to the two-compartment open model and showed linear pharmacokinetics.

Published
2005

6. [Pharmacokinetics of patchouli alcohol and patchouli alcohol in patchouli oil after iv administrated to rats]

Author

Fu-chuan, Yang, Li-zhen, Xu, Zhong-mei, Zou, and Shi-lin, Yang

Subjects
Male, Rats, Sprague-Dawley, Lamiaceae, Plants, Medicinal, Area Under Curve, Injections, Intravenous, Oils, Volatile, Animals, Sesquiterpenes, Rats
Abstract

To develop a capillary gas chromatographic method for the determination and pharmacokinetic study of patchouli alcohol in rat plasma after iv administration.The drug was extracted with ethyl acetate. Eugenol was used as internal standard. The separation was carried out on a HP-5MS quartz capillary column, with high-purity nitrogen as carrier gas and flame ionization detector (FID) as detector. The column temperature was maintained at 80 degrees C for 1 min and then programmed to 200 degrees C at a rate of 15 degrees C x min(-1); it was held at 200 degrees C for 1 min, and then programmed to 290 degrees C at a rate of 60 degrees C x min(-1); the final temperature was held for 1 min. The temperature of both injector and detector was set at 290 degrees C.The standard curve was linear from 25 to 5 000 microg x L(-1) in rat plasma. The recovery of this method was from 90.0% to 110.0% with satisfactory relative standard deviation (RSD) less than 10.0%. The pharmacokinetic parameters demonstrated patchouli alcohol were consistent with the two-compartment open model and showed linear pharmacokinetics. The T1/2beta, AUC and MRT of patchouli alcohol in patchouli oil were all higher than that of patchouli alcohol.This method is quick, precise and reliable. The pharmacokinetics of patchouli alcohol is different from that of patchouli alcohol in patchouli oil.

Published
2004

11. Competitive binding between Seryl-tRNA synthetase/YY1 complex and NFKB1 at the distal segment results in differential regulation of human vegfa promoter activity during angiogenesis.

Author

Fu CY, Wang PC, and Tsai HJ

Subjects
Animals, Binding, Competitive, Catalytic Domain, HEK293 Cells, Humans, Serine-tRNA Ligase chemistry, Vascular Endothelial Growth Factor A biosynthesis, Zebrafish embryology, Gene Expression Regulation, NF-kappa B p50 Subunit metabolism, Neovascularization, Physiologic genetics, Promoter Regions, Genetic, Serine-tRNA Ligase metabolism, Vascular Endothelial Growth Factor A genetics, YY1 Transcription Factor metabolism
Abstract

Vascular endothelial growth factor (VEGF) plays a pivotal role in angiogenesis. Previous studies focused on transcriptional regulation modulated by proximal upstream cis-regulatory elements (CREs) of the human vegfa promoter. However, we hypothesized that distal upstream CREs may also be involved in controlling vegfa transcription. In this study, we found that the catalytic domain of Seryl-tRNA synthetase (SerRS) interacted with transcription factor Yin Yang 1 (YY1) to form a SerRS/YY1 complex that negatively controls vegfa promoter activity through binding distal CREs at -4654 to -4623 of vegfa. Particularly, we demonstrated that the -4654 to -4623 segment, which predominantly controls vegfa promoter activity, is involved in competitive binding between SerRS/YY1 complex and NFKB1. We further showed that VEGFA protein and blood vessel development were reduced by overexpression of either SerRS or YY1, but enhanced by the knockdown of either SerRS or yy1. In contrast, these same parameters were enhanced by overexpression of NFKB1, but reduced by knockdown of nfkb1. Therefore, we suggested that SerRS does not bind DNA directly but form a SerRS/YY1 complex, which functions as a negative effector to regulate vegfa transcription through binding at the distal CREs; while NFKB1 serves as a positive effector through competing with SerRS/YY1 binding at the overlapping CREs., (© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.)

Published
2017
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