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This document is a letter published in the journal Clinical Chemistry & Laboratory Medicine. It discusses a clinical pilot study on the automation of library preparation for next-generation sequencing (NGS) using microfluidics. The study focuses on the screening of somatic rearrangements of Immunoglobulin (IG) genes in patients with acute lymphoblastic leukemia (ALL). The researchers used a centrifugal microfluidic cartridge and a modified commercially available player for the automation process. The results showed that the automated library preparation using microfluidics was comparable to manual library preparation in terms of coverage and detection of IGH-VJ rearrangements. The study suggests that centrifugal microfluidic automation can reduce complexity and staff hands-on time in a clinical setup for library preparation. However, the choice of automation system depends on the throughput numbers of the laboratory. The researchers also mention the possibility of manufacturing cartridges for other lymphoid malignancies and the potential for MRD quantification by NGS. [Extracted from the article]

The article discusses the use of next-generation sequencing and high DNA input to detect measurable residual disease (MRD) in the peripheral blood of patients with B-cell precursor acute lymphoblastic leukemia (ALL). Traditional MRD assessment in bone marrow samples may miss MRD in peripheral blood, but modern molecular methods can enhance detection. The study found that adjusting traditional MRD assessment strategies can improve MRD detection in peripheral blood, but it may not fully replace bone marrow monitoring. The findings suggest that high DNA input and next-generation sequencing can increase the sensitivity of MRD detection in peripheral blood, especially in cases where bone marrow samples are unavailable. [Extracted from the article]

Psychodynamic models will be presented after a critical discussion of the biomedical-psychiatric model of attention deficit and hyperactivity disorder (dimensional diagnostics, genetic disorder, dopamine deficiency, neuronal developmental disorder). Research findings on early mother-child interactions refer to specific psychosocial developmental conditions amongst children who develop ADHD. Factors which lead to gene expression can be traced here. A psychodynamic-oriented developmental psychotherapy which centres on regulatory procedures, de-centration, mentalisation, de-somatisation and symbolisation of treated notifications is helpful, particularly amongst children with complex disorders. The underlying conflicts and dealing with them are thus elucidated in the presentation of a treatment.