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Selection leading to adaptation to interactions may generate rapid evolutionary feedbacks and drive diversification of species interactions. The challenge is to understand how the many traits of interacting species combine to shape local adaptation in ways directly or indirectly resulting in diversification. We used the well-studied interactions between Lithophragma plants (Saxifragaceae) and Greya moths (Prodoxidae) to evaluate how plants and moths together contributed to local divergence in pollination efficacy. Specifically, we studied L. bolanderi and its two specialized Greya moth pollinators in two contrasting environments in the Sierra Nevada in California. Both moths pollinate L. bolanderi during nectaring, one of them–G. politella–also while ovipositing through the floral corolla into the ovary. First, field surveys of floral visitors and the presence of G. politella eggs and larvae in developing capsules showed that one population was visited only by G. politella and few other pollinators, whereas the other was visited by both Greya species and other pollinators. Second, L. bolanderi in these two natural populations differed in several floral traits putatively important for pollination efficacy. Third, laboratory experiments with greenhouse-grown plants and field-collected moths showed that L. bolanderi was more efficiently pollinated by local compared to nonlocal nectaring moths of both species. Pollination efficacy of ovipositing G. politella was also higher for local moths for the L. bolanderi population, which relies more heavily on this species in nature. Finally, time-lapse photography in the laboratory showed that G. politella from different populations differed in oviposition behavior, suggesting the potential for local adaptation also among Greya populations. Collectively, our results are a rare example of components of local adaptation contributing to divergence in pollination efficacy in a coevolving interaction and, thus, provide insights into

Dermatophytes are common pathogens in superficial mycoses that are routinely identified by culture or PCR analysis of freshly collected skin, nail or hair specimens. Although clinical samples are normally processed without delay, practical or research issues may necessitate sample storage until later analysis. However, the influence of extended sample storage on the ability to recover fungi by culture vs. PCR analysis has not been specifically studied. Here, a total of 172 dermatological samples collected from 2013-2015 were examined before and after refrigerated storage at 4 degrees C for 10.2-32.3 (mean 25.6) months. By culture, 35% of the dermatophyte-containing fresh samples remained positive at reexamination. At species level, only 19% of initially Trichophyton rubrum-positive samples yielded a positive result after refrigeration, whereas few samples containing Trichophyton violaceum, Microsporum canis or Microsporum audouinii remained culture-positive. Using PCR, 76% of dermatophyte DNA-positive fresh samples were still positive at re-analysis. Notably, 92% of the samples targeted by the T. rubrum DNA primer remained positive after storage. Hence, PCR analysis is more favourable than cultivation with regard to the detectability of dermatophytes in long-term refrigerated clinical samples.

The world s challenges of climate change, damage to ecosystems, and social and health inequalities require changes in human behaviours at every level of organisation, among governments, business, communities, and individuals. An important question is how behaviour change can be enabled and supported at the scale and speed required. The research reported in this paper describes important lessons for good practice in changing contexts to modify behaviours for a triple win for health, equity and environmental sustainability. Authors synthesised learning from qualitative, quantitative and cost benefit evaluations of 15 case studies conducted in 12 countries in Europe. The case studies address ways of living (green spaces and energy efficient housing), moving (active transport) and consuming (healthy and sustainable diets) that support the triple win. Ten lessons for good practice were identified. These include bringing a triple win mindset to policy and practice in planning interventions, with potential to improve environmental sustainability, health and equity at the same time. The lessons for good practice are intended to support governmental and non-governmental actors, practitioners and researchers planning to work across sectors to achieve mutual benefits for health and environmental sustainability and in particular to benefit poorer and more socio-economically disadvantaged groups. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

The relative role of natural selection and genetic drift in evolution is a major topic of debate in evolutionary biology. Most knowledge spring from a small group of organisms and originate from before it was possible to generate genome-wide data on genetic variation. Hence, it is necessary to extend to a larger number of taxonomic groups, descriptive and hypothesis-based research aiming at understanding the proximate and ultimate mechanisms underlying both levels of genetic polymorphism and the efficiency of natural selection. In this study, we used data from 60 whole-genome resequenced individuals of three cryptic butterfly species (Leptidea sp.), together with novel gene annotation information and population recombination data. We characterized the overall prevalence of natural selection and investigated the effects of mutation and linked selection on regional variation in nucleotide diversity. Our analyses showed that genome-wide diversity and rate of adaptive substitutions were comparatively low, whereas nonsynonymous to synonymous polymorphism and substitution levels were comparatively high in Leptidea, suggesting small long-term effective population sizes. Still, negative selection on linked sites (background selection) has resulted in reduced nucleotide diversity in regions with relatively high gene density and low recombination rate. We also found a significant effect of mutation rate variation on levels of polymorphism. Finally, there were considerable population differences in levels of genetic diversity and pervasiveness of selection against slightly deleterious alleles, in line with expectations from differences in estimated effective population sizes.

Chromosomal rearrangements (e.g., fusions/fissions) have the potential to drive speciation. However, their accumulation in a population is generally viewed as unlikely, because chromosomal heterozygosity should lead to meiotic problems and aneuploid gametes. Canonical meiosis involves segregation of homologous chromosomes in meiosis I and sister chromatid segregation during meiosis II. In organisms with holocentric chromosomes, which are characterized by kinetic activity distributed along almost the entire chromosome length, this order may be inverted depending on their metaphase I orientation. Here we analyzed the evolutionary role of this intrinsic versatility of holocentric chromosomes, which is not available to monocentric ones, by studying F1 to F4 hybrids between two chromosomal races of the Wood White butterfly (Leptidea sinapis), separated by at least 24 chromosomal fusions/fissions. We found that these chromosomal rearrangements resulted in multiple meiotic multivalents, and, contrary to the theoretical prediction, the hybrids displayed relatively high reproductive fitness (42% of that of the control lines) and regular behavior of meiotic chromosomes. In the hybrids, we also discovered inverted meiosis, in which the first and critical stage of chromosome number reduction was replaced by the less risky stage of sister chromatid separation. We hypothesize that the ability to invert the order of the main meiotic events facilitates proper chromosome segregation and hence rescues fertility and viability in chromosomal hybrids, potentially promoting dynamic karyotype evolution and chromosomal speciation.

1. A high rate of reproduction may be costly if ecological factors limit immediate reproductive output as a fast metabolism compromises own future survival. Individuals with more reserves need more time and opportunity to realize their reproductive potential. Theory therefore predicts that the reproductive rate, defined as the investment in early reproduction in proportion to total potential, should decrease with body size within species. 2. However, metabolic constraints on body size- and temperature-dependent biological rates may impede biophysical adaptation. Furthermore, the sequential manner resources that are allocated to somatic vs. reproductive tissue during ontogeny may, when juveniles develop in unpredictable environments, further contribute to non-adaptive variation in adult reproductive rates. 3. With a model on female egg laying in insects, we demonstrate how variation in body reserves is predicted to affect reproductive rate under different ecological scenarios. Small females always have higher reproductive rates but shorter lifespans. However, incorporation of female host selectivity leads to more similar reproductive rates among female size classes, and oviposition behaviour is predicted to co-evolve with reproductive rate, resulting in small females being more selective in their choice and gaining relatively more from it. 4. We fed simulations with data on the butterfly Pararge aegeria to compare model predictions with reproductive rates of wild butterflies. However, simulated reproductive allometry was a poor predictor of that observed. Instead, reproductive rates were better explained as a product of metabolic constraints on rates of egg maturation, and an empirically derived positive allometry between reproductive potential and size. However, fitness is insensitive to moderate deviations in reproductive rate when oviposition behaviour is allowed to co-evolve in the simulations, suggesting that behavioural compensation may mitigate putative metabo

EUROPROBE-related seismic reflection surveys in the Middle Urals, Russia (latitude 56-62°) since 1993 have led to an increased understanding of the crustal structure and tectonic evolution of this region. A 400 km long profile now extends from the foreland basin in the west well into the West Siberian Basin in the east. Bivergent structures characterize the upper crust of the Uralide orogen, whereas the middle and lower crust generally contain gently west-dipping reflections. A crustal root is imaged down to almost 60 km beneath the exposed Urals. Below the foreland and the West Siberian Basin the lower crustal reflectivity is pronounced and the Moho lies at a depth of 40-45 km. Below the foreland on the recently acquired Serebrianka-Beriozovka profile, two sets of late arriving (20-25 s) reflections are present. One set reflects from a zone in the mantle at about 60-70 km depth that strikes ENE and dips about 45° to the SSE. The other set may represent imbricated lower crust. Major events during the Palaeozoic tectonic evolution of the Middle Urals were: continental and oceanic rifting (Late Cambrian to Early Ordovician); development of a passive continental margin (Mid-Ordovician to Mid-Carboniferous); intra-oceanic subduction below the Tagil arc (Silurian to Devonian); east-dipping subduction of the Baltica plate (Silurian to Early Devonian); possible subduction reversal with formation of the Alapaevsk island arc and the Krasnoturjinsk-Petrokamensk active continental margin (Devonian to Early Carboniferous); active building of a mountain belt and intrusion of collision-related granitic plutons (Carboniferous to Permian).

New deep seismic reflection data provide images of the crust and uppermost mantle underlying the eastern Middle Urals and adjacent West Siberian Basin. Distinct truncations of reflections delineate the late-orogenic strike-slip Sisert Fault extending vertically to ∼28 km depth, and two gently E-dipping reflection zones, traceable to 15–18 km depth, probably represent normal faults associated with the opening of the West Siberian Basin. A possible remnant Palaeozoic subduction zone in the lower crust under the West Siberian Basin is visible as a gently SW-dipping zone of pronounced reflectivity truncated by the Moho. Continuity of shallow to intermediate-depth reflections suggest that Palaeozoic accreted island-arc terranes and overlying molasse sequences exposed in the hinterland of the Urals form the basement for Triassic and younger deposits in the West Siberian Basin. A highly reflective lower crust overlies a transparent mantle at about 43 km depth along the entire 100 km long seismic reflection section, suggesting that the lower crust and Moho below the eastern Middle Urals and West Siberian Basin have the same origin.

As a contribution to Europrobe's seismic reflection profiling in the Urals (ESRU)project, three overlapping seismic reflection data sets were acquired in the Middle Urals. A 56 km long profile was registered over the Europe-Asia suture, two 25 km long intersecting profiles were collected over the Urals superdeep borehole (SG4), and an 80 km long profile was recorded eastward extending east toward the West Siberian Basin. Reflections on the seismic sections delineate several major middle to late Paleozoic thrust zones in the upper crust. These thrust zones have a bivergent geometry with westerly vergence west of the Uralian orogenic axis and easterly vergence to the east. The principal terrane boundaries are the Main Uralian Thrust Fault in the west and the Trans- Uralian Thrust Zone in the east. Normal faults are spatially associated with former thrust faults, or they crosscut them. The thrust and normal faults can be confidently correlated with surface geological features. Near-vertical and wide-angle seismic reflection profiling reveals thickening of the crust from about 45 km to approximately 53 km below the central axis of the Urals. East and west of the root zone, the lower crust is reflective, particularly toward the West Siberian Basin. We interpret the reflectivity of the crust below the East European Craton as pre-Uralian, whereas that toward the West Siberian Basin is interpreted as late orogenic. Although the principal tectonic features imaged by the seismic sections are probably of Paleozoic age, a post-Paleozoic origin for the lower crustal reflectivity in the east cannot be ruled out.

In the Middle Urals, volcanic-are and back-are basin rocks of Ordovician to Devonian age occur in the Tagil Synform. These outboard terranes were thrust westwards in the late Carboniferous onto continental margin associations of late Proterozoic and Palae, Addresses: Friberg M, Uppsala Univ, Dept Geophys, Villavagen 16, S-75236 Uppsala, Sweden. Uppsala Univ, Dept Geophys, S-75236 Uppsala, Sweden. Urals Geol Mapping Expedit, UralGeolCom, Ekaterinburg, Sverdlovsk Dist, Russia.

Part of urn:nbn:se:su:diva-7498