131 results on '"Frederic Laurent"'
Search Results
2. Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent feature
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Ines Rodrigues Lopes, Laura Maria Alcantara, Ricardo Jorge Silva, Jerome Josse, Elena Pedrero Vega, Ana Marina Cabrerizo, Melanie Bonhomme, Daniel Lopez, Frederic Laurent, Francois Vandenesch, Miguel Mano, and Ana Eulalio
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Science - Abstract
Staphylococcus aureus is increasingly recognized as a facultative intracellular pathogen, but it is unclear whether the intracellular lifestyle is a general feature or is restricted to some isolates. Here, Rodrigues Lopes et al. profile the interaction of 191 clinical isolates with four host cell types over time, showing that almost all isolates are internalized and that a large fraction replicate and persist within host cells.
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- 2022
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3. Coxiella burnetti prosthetic joint infection in an immunocompromised woman: iterative surgeries, prolonged ofloxacin-rifampin treatment and complex reconstruction were needed for the cure
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Patrick Miailhes, Anne Conrad, Chantal Sobas, Frederic Laurent, Sebastien Lustig, Tristan Ferry, and on behalf of the Lyon BJI study group
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Q fever ,Prosthetic joint infection ,Rheumatoid arthritis ,Anti-TNF-α ,Coxiella burnetii ,Orthopedic surgery ,RD701-811 - Abstract
Abstract Background Q fever is a zoonotic disease caused by the bacterium Coxiella burnetii, a strictly intracellular pathogen that can cause acute and chronic infection. Chronic Q fever can occur in immunocompetent as well as in immuno-compromised hosts, as a persistent localized infection. The main localizations are endocardial, vascular and, less frequently, osteoarticular. The most frequent osteoarticular form is spondyliscitis. Recommended treatment is combined doxycycline and hydroxychloroquine for 18 months, with cotrimoxazole as another option. Coxiella burnetti infection has been implicated in rare cases of prosthetic joint infection (PJI), and the medical and surgical management and outcome in such cases have been little reported. Case presentation We report an unusual case of chronic Q fever involving a hip arthroplasty in an immunocompromised woman treated with tumor necrosis factor (TNF)-α blockers for rheumatoid arthritis. Numerous surgical procedures (explantation, “second look”, femoral resection and revision by megaprosthesis), modification of the immunosuppressant therapy and switch from doxycycline-hydroxychloroquine to prolonged ofloxacin-rifampin combination therapy were needed to achieve reconstruction and treat the PJI, with a follow-up of 7 years. Conclusions Coxiella burnetti PJI is a complex infection that requires dedicated management in an experienced reference center. Combined use of ofloxacin-rifampin can be effective.
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- 2021
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4. Impact on the Gut Microbiota of Intensive and Prolonged Antimicrobial Therapy in Patients With Bone and Joint Infection
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Benoît Levast, Nicolas Benech, Cyrielle Gasc, Cécile Batailler, Eric Senneville, Sébastien Lustig, Cécile Pouderoux, David Boutoille, Lilia Boucinha, Frederic-Antoine Dauchy, Valérie Zeller, Marianne Maynard, Charles Cazanave, Thanh-Thuy Le Thi, Jérôme Josse, Joël Doré, Frederic Laurent, and Tristan Ferry
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gut microbiota ,antimicrobial therapy ,antibiotics ,bone and joint infection ,dysbiosis ,Medicine (General) ,R5-920 - Abstract
There is a growing interest in the potentially deleterious impact of antibiotics on gut microbiota. Patients with bone and joint infection (BJI) require prolonged treatment that may impact significantly the gut microbiota. We collected samples from patients with BJI at baseline, end of antibiotics (EOT), and 2 weeks after antibiotic withdrawal (follow-up, FU) in a multicenter prospective cohort in France. Microbiota composition was determined by shotgun metagenomic sequencing. Fecal markers of gut permeability and inflammation as well as multi-drug-resistant bacteria (MDRB) and Clostridioides difficile carriage were assessed at each time point. Sixty-two patients were enrolled: 27 native BJI, 14 osteosynthesis-related BJI, and 21 prosthetic joint infections (PJI). At EOT, there was a significant loss of alpha-diversity that recovered at FU in patients with native BJI and PJI, but not in patients with osteosynthesis-related BJI. At EOT, we observed an increase of Proteobacteria and Bacteroidetes that partially recovered at FU. The principal component analysis (PCoA) of the Bray–Curtis distance showed a significant change of the gut microbiota at the end of treatment compared to baseline that only partially recover at FU. Microbiota composition at FU does not differ significantly at the genus level when comparing patients treated for 6 weeks vs. those treated for 12 weeks. The use of fluoroquinolones was not associated with a lower Shannon index at the end of treatment; however, the PCoA of the Bray–Curtis distance showed a significant change at EOT, compared to baseline, that fully recovered at FU. Levels of fecal neopterin were negatively correlated with the Shannon index along with the follow-up (r2 = 0.17; p < 0.0001). The PCoA analysis of the Bray–Curtis distance shows that patients with an elevated plasma level of C-reactive protein (≥5 mg/L) at EOT had a distinct gut microbial composition compared to others. MDRB and C. difficile acquisition at EOT and FU represented 20% (7/35) and 37.1% (13/35) of all MDRB/C. difficile-free patients at the beginning of the study, respectively. In patients with BJI, antibiotics altered the gut microbiota diversity and composition with only partial recovery, mucosal inflammation, and permeability and acquisition of MDRB carriage. Microbiome interventions should be explored in patients with BJI to address these issues.
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- 2021
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5. Pseudomonas aeruginosa Implant-Associated Bone and Joint Infections: Experience in a Regional Reference Center in France
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Matteo Cerioli, Cécile Batailler, Anne Conrad, Sandrine Roux, Thomas Perpoint, Agathe Becker, Claire Triffault-Fillit, Sebastien Lustig, Michel-Henri Fessy, Frederic Laurent, Florent Valour, Christian Chidiac, and Tristan Ferry
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pseudomonas ,osteomyelitis ,ciprofloxacin ,implant-associated bone infections (IABI) ,bone and joint infection ,Medicine (General) ,R5-920 - Abstract
Background:P. aeruginosa implant-associated bone and joint infections (BJI) is considered to be one of the most difficult to treat BJI. The data focusing specifically on this pathogen are sparse, and it seems difficult to extrapolate the results obtained with Enterobacteriaceae.Methods: We performed a retrospective observation study of all P. aeruginosa implant-associated BJI diagnosed at our institution from 2011 to 2018. We defined failure as any type of relapse, including persistence of the same P. aeruginosa, superinfection by another organism(s) or any other cause of relapse such as the need for a subsequent surgery. Nonparametric statistical methods were used to compare the study groups and Kaplan-Meier curves and multivariate Cox analysis and were used to detect determinants associated with treatment failure.Results: A total of 90 patients (62% men, median age 60 years IQR 47–72) including 30 (33%) prosthetic-joint infections and 60 (66%) other implant-associated BJIs were studied. Most of them were acute (62%). During the prolonged follow-up, (median 20 months; IQR 9–37), 23 patients (26%) experienced treatment failure. Optimal surgical treatment (DAIR for acute forms, explantation, 1-stage or 2-stage exchange for others) was significantly associated with a higher success rate in the univariate analysis (p = 0.003). Sixty-four (71%) patients received effective initial treatment against P. aeruginosa administered and 81 of them (90%) did for at least 3 weeks: both these parameters correlated with a higher success rate. In the multivariate Cox-analysis optimal surgical treatment, IV effective treatment of at least 3 weeks and treatment with ciprofloxacin for at least 3 months proved to be independently associated to a better outcome in patients with P. aeruginosa implant-associated BJI.Conclusion:P. aeruginosa implant-associated BJI is one of the most difficult-to-treat BJI, with a strong impact on the prognosis of the surgical strategy. An effective initial IV antibiotic treatment for at least 3 weeks seems to be required, followed by oral ciprofloxacin for a total duration of 3 months.
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- 2020
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6. Community-Acquired Staphylococcus argenteus Sequence Type 2250 Bone and Joint Infection, France, 2017
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Josselin Rigaill, Florence Grattard, Sylvain Grange, Fabien Forest, Elie Haddad, Anne Carricajo, Anne Tristan, Frederic Laurent, Elisabeth Botelho-Nevers, and Paul O. Verhoeven
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Staphylococcus argenteus ,Staphylococcus aureus ,bacteria ,sequence type ,ST2250 ,bone and joint infection ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We report a rare case of Staphylococcus argenteus bone and joint infection in a 9-year-old boy in France. His finger arthritis was complicated by osteitis 5 weeks later, which resulted in a secondary intervention. This case indicates the virulence of S. argenteus, an emerging pathogen whose clinical effects are poorly described.
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- 2018
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7. Understanding the Virulence of Staphylococcus pseudintermedius: A Major Role of Pore-Forming Toxins
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Yousef Maali, Cédric Badiou, Patrícia Martins-Simões, Elisabeth Hodille, Michele Bes, François Vandenesch, Gérard Lina, Alan Diot, Frederic Laurent, and Sophie Trouillet-Assant
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Staphylococcus pseudintermedius ,pore-forming toxins ,phenol-soluble modulins ,leukotoxin Luk-I ,CXCR2 receptor ,cytotoxicity ,Microbiology ,QR1-502 - Abstract
Staphylococcus pseudintermedius is responsible for severe and necrotizing infections in humans and dogs. Contrary to S. aureus, the pathophysiological mechanisms involved in this virulence are incompletely understood. We previously showed the intracellular cytotoxicity induced after internalization of S. pseudintermedius. Herein, we aimed to identify the virulence factors responsible for this cytotoxic activity. After addition of filtered S. pseudintermedius supernatants in culture cell media, MG63 cells, used as representative of non-professional phagocytic cells (NPPc), released a high level of LDH, indicating that the cytotoxicity was mainly mediated by secreted factors. Accordingly, we focused our attention on S. pseudintermedius toxins. In silico analysis found the presence of two PSMs (δ-toxin and PSMε) as well as Luk-I leukotoxin, the presence of which was confirmed by PCR in all clinical strains tested (n = 17). Recombinant Luk-I leukotoxin had no cytotoxic activity on NPPc but the ectopic expression of the CXCR2 receptor in U937 cells conferred cytotoxity to Luk-I. This is in agreement with the lack of Luk-I effect on NPPc and the previous report of Luk-I cytoxic activity on immune cells. Contrary to Luk-I, synthetic δ-toxin and PSMε had a strong cytotoxic activity on NPPc. The secretion of δ-toxin and PSMε at cytotoxic concentrations by S. pseudintermedius in culture supernatant was confirmed by HPLC-MS. In addition, the supplementation of such supernatants with human serum, known to inhibit PSM, induced a complete abolition of cytotoxicity which indicates that PSMs are the key players in the cytotoxic phenotype of NPPc. The results suggest that the severity of S. pseudintermedius infections is, at least in part, explained by a combined action of Luk-I that specifically targets immune cells expressing the CXCR2 receptor, and PSMs that disrupt cell membranes whatever the cell types. The present study strengthens the key role of PSMs in virulence of the different species belonging to Staphylococcus genus.
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- 2018
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8. Routine Whole-Genome Sequencing for Outbreak Investigations of Staphylococcus aureus in a National Reference Center
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Geraldine Durand, Fabien Javerliat, Michèle Bes, Jean-Baptiste Veyrieras, Ghislaine Guigon, Nathalie Mugnier, Stéphane Schicklin, Gaël Kaneko, Emmanuelle Santiago-Allexant, Coralie Bouchiat, Patrícia Martins-Simões, Frederic Laurent, Alex Van Belkum, François Vandenesch, and Anne Tristan
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Staphylococcus aureus ,whole-genome sequencing ,national reference center ,outbreak investigation ,bioinformatics pipeline ,de novo assembly ,Microbiology ,QR1-502 - Abstract
The French National Reference Center for Staphylococci currently uses DNA arrays and spa typing for the initial epidemiological characterization of Staphylococcus aureus strains. We here describe the use of whole-genome sequencing (WGS) to investigate retrospectively four distinct and virulent S. aureus lineages [clonal complexes (CCs): CC1, CC5, CC8, CC30] involved in hospital and community outbreaks or sporadic infections in France. We used a WGS bioinformatics pipeline based on de novo assembly (reference-free approach), single nucleotide polymorphism analysis, and on the inclusion of epidemiological markers. We examined the phylogeographic diversity of the French dominant hospital-acquired CC8-MRSA (methicillin-resistant S. aureus) Lyon clone through WGS analysis which did not demonstrate evidence of large-scale geographic clustering. We analyzed sporadic cases along with two outbreaks of a CC1-MSSA (methicillin-susceptible S. aureus) clone containing the Panton–Valentine leukocidin (PVL) and results showed that two sporadic cases were closely related. We investigated an outbreak of PVL-positive CC30-MSSA in a school environment and were able to reconstruct the transmission history between eight families. We explored different outbreaks among newborns due to the CC5-MRSA Geraldine clone and we found evidence of an unsuspected link between two otherwise distinct outbreaks. Here, WGS provides the resolving power to disprove transmission events indicated by conventional methods (same sequence type, spa type, toxin profile, and antibiotic resistance profile) and, most importantly, WGS can reveal unsuspected transmission events. Therefore, WGS allows to better describe and understand outbreaks and (inter-)national dissemination of S. aureus lineages. Our findings underscore the importance of adding WGS for (inter-)national surveillance of infections caused by virulent clones of S. aureus but also substantiate the fact that technological optimization at the bioinformatics level is still urgently needed for routine use. However, the greatest limitation of WGS analysis is the completeness and the correctness of the reference database being used and the conversion of floods of data into actionable results. The WGS bioinformatics pipeline (EpiSeqTM) we used here can easily generate a uniform database and associated metadata for epidemiological applications.
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- 2018
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9. Demography and Intercontinental Spread of the USA300 Community-Acquired Methicillin-Resistant Staphylococcus aureus Lineage
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Philippe Glaser, Patrícia Martins-Simões, Adrien Villain, Maxime Barbier, Anne Tristan, Christiane Bouchier, Laurence Ma, Michele Bes, Frederic Laurent, Didier Guillemot, Thierry Wirth, and François Vandenesch
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Microbiology ,QR1-502 - Abstract
ABSTRACT Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was recognized worldwide during the 1990s; in less than a decade, several genetically distinct CA-MRSA lineages carrying Panton-Valentine leukocidin genes have emerged on every continent. Most notably, in the United States, the sequence type 18-IV (ST8-IV) clone known as USA300 has become highly prevalent, outcompeting methicillin-susceptible S. aureus (MSSA) and other MRSA strains in both community and hospital settings. CA-MRSA bacteria are much less prevalent in Europe, where the European ST80-IV European CA-MRSA clone, USA300 CA-MRSA strains, and other lineages, such as ST22-IV, coexist. The question that arises is whether the USA300 CA-MRSA present in Europe (i) was imported once or on very few occasions, followed by a broad geographic spread, anticipating an increased prevalence in the future, or (ii) derived from multiple importations with limited spreading success. In the present study, we applied whole-genome sequencing to a collection of French USA300 CA-MRSA strains responsible for sporadic cases and micro-outbreaks over the past decade and United States ST8 MSSA and MRSA isolates. Genome-wide phylogenetic analysis demonstrated that the population structure of the French isolates is the product of multiple introductions dating back to the onset of the USA300 CA-MRSA clone in North America. Coalescent-based demography of the USA300 lineage shows that a strong expansion occurred during the 1990s concomitant with the acquisition of the arginine catabolic mobile element and antibiotic resistance, followed by a sharp decline initiated around 2008, reminiscent of the rise-and-fall pattern previously observed in the ST80 lineage. A future expansion of the USA300 lineage in Europe is therefore very unlikely. IMPORTANCE To trace the origin, evolution, and dissemination pattern of the USA300 CA-MRSA clone in France, we sequenced a collection of strains of this lineage from cases reported in France in the last decade and compared them with 431 ST8 strains from the United States. We determined that the French CA-MRSA USA300 sporadic and micro-outbreak isolates resulted from multiple independent introductions of the USA300 North American lineage. At a global level, in the transition from an MSSA lineage to a successful CA-MRSA clone, it first became resistant to multiple antibiotics and acquired the arginine catabolic mobile element and subsequently acquired resistance to fluoroquinolones, and these two steps were associated with a dramatic demographic expansion. This expansion was followed by the current stabilization and expected decline of this lineage. These findings highlight the significance of horizontal gene acquisitions and point mutations in the success of such disseminated clones and illustrate their cyclic and sporadic life cycle.
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- 2016
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10. MRSA Harboring mecA Variant Gene mecC, France
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Frederic Laurent, Hubert Chardon, Marisa Haenni, Michele Bes, Marie-Elisabeth Reverdy, Jean-Yves Madec, Evelyne Lagier, François Vandenesch, and Anne Tristan
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methicillin-resistant Staphylococcus aureus ,MRSA ,bacteria ,mecA ,mecC ,mecALGA251 ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We describe human cases and clustered animal cases of mecALGA251–positive methicillin-resistant Staphylococcus aureus in France. Our report confirms that this new variant has a large distribution in Europe. It may represent a public health threat because phenotypic and genotypic tests seem unable to detect this new resistance mechanism.
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- 2012
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11. Origin and Evolution of European Community-Acquired Methicillin-Resistant Staphylococcus aureus
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Marc Stegger, Thierry Wirth, Paal S. Andersen, Robert L. Skov, Anna De Grassi, Patricia Martins Simões, Anne Tristan, Andreas Petersen, Maliha Aziz, Kristoffer Kiil, Ivana Cirković, Edet E. Udo, Rosa del Campo, Jaana Vuopio-Varkila, Norazah Ahmad, Sima Tokajian, Georg Peters, Frieder Schaumburg, Barbro Olsson-Liljequist, Michael Givskov, Elizabeth E. Driebe, Henrik E. Vigh, Adebayo Shittu, Nadjia Ramdani-Bougessa, Jean-Philippe Rasigade, Lance B. Price, Francois Vandenesch, Anders R. Larsen, and Frederic Laurent
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Microbiology ,QR1-502 - Abstract
ABSTRACT Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was recognized in Europe and worldwide in the late 1990s. Within a decade, several genetically and geographically distinct CA-MRSA lineages carrying the small SCCmec type IV and V genetic elements and the Panton-Valentine leukocidin (PVL) emerged around the world. In Europe, the predominant CA-MRSA strain belongs to clonal complex 80 (CC80) and is resistant to kanamycin/amikacin and fusidic acid. CC80 was first reported in 1993 but was relatively rare until the late 1990s. It has since been identified throughout North Africa, the Middle East, and Europe, with recent sporadic reports in sub-Saharan Africa. While strongly associated with skin and soft tissue infections, it is rarely found among asymptomatic carriers. Methicillin-sensitive S. aureus (MSSA) CC80 strains are extremely rare except in sub-Saharan Africa. In the current study, we applied whole-genome sequencing to a global collection of both MSSA and MRSA CC80 isolates. Phylogenetic analyses strongly suggest that the European epidemic CA-MRSA lineage is derived from a PVL-positive MSSA ancestor from sub-Saharan Africa. Moreover, the tree topology suggests a single acquisition of both the SCCmec element and a plasmid encoding the fusidic acid resistance determinant. Four canonical SNPs distinguish the derived CA-MRSA lineage and include a nonsynonymous mutation in accessory gene regulator C (agrC). These changes were associated with a star-like expansion into Europe, the Middle East, and North Africa in the early 1990s, including multiple cases of cross-continent imports likely driven by human migrations. IMPORTANCE With increasing levels of CA-MRSA reported from most parts of the Western world, there is a great interest in understanding the origin and factors associated with the emergence of these epidemic lineages. To trace the origin, evolution, and dissemination pattern of the European CA-MRSA clone (CC80), we sequenced a global collection of strains of the S. aureus CC80 lineage. Our study determined that a single descendant of a PVL-positive methicillin-sensitive ancestor circulating in sub-Saharan Africa rose to become the dominant CA-MRSA clone in Europe, the Middle East, and North Africa. In the transition from a methicillin-susceptible lineage to a successful CA-MRSA clone, it simultaneously became resistant to fusidic acid, a widely used antibiotic for skin and soft tissue infections, thus demonstrating the importance of antibiotic selection in the success of this clone. This finding furthermore highlights the significance of horizontal gene acquisitions and underscores the combined importance of these factors for the success of CA-MRSA.
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- 2014
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12. Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock
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Lance B. Price, Marc Stegger, Henrik Hasman, Maliha Aziz, Jesper Larsen, Paal Skytt Andersen, Talima Pearson, Andrew E. Waters, Jeffrey T. Foster, James Schupp, John Gillece, Elizabeth Driebe, Cindy M. Liu, Burkhard Springer, Irena Zdovc, Antonio Battisti, Alessia Franco, Jacek Żmudzki, Stefan Schwarz, Patrick Butaye, Eric Jouy, Constanca Pomba, M. Concepción Porrero, Raymond Ruimy, Tara C. Smith, D. Ashley Robinson, J. Scott Weese, Carmen Sofia Arriola, Fangyou Yu, Frederic Laurent, Paul Keim, Robert Skov, and Frank M. Aarestrup
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Microbiology ,QR1-502 - Published
- 2013
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13. Genome analysis of Staphylococcus aureus ST291, a double locus variant of ST398, reveals a distinct genetic lineage.
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Marc Stegger, Maliha Aziz, Tomasz Chroboczek, Lance B Price, Troels Ronco, Kristoffer Kiil, Robert L Skov, Frederic Laurent, and Paal S Andersen
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Medicine ,Science - Abstract
Staphylococcus aureus ST291 has been reported as a homologue recombinant double locus variant of the livestock associated S. aureus ST398. However, whole genome sequencing show that ST291 is a unique genetic lineage with highly variable content within its accessory genome compared to both human and livestock associated genome sequenced CC398s.
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- 2013
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14. Clonal complex 398 methicillin susceptible Staphylococcus aureus: a frequent unspecialized human pathogen with specific phenotypic and genotypic characteristics.
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Tomasz Chroboczek, Sandrine Boisset, Jean-Philippe Rasigade, Anne Tristan, Michele Bes, Helene Meugnier, François Vandenesch, Jerome Etienne, and Frederic Laurent
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Medicine ,Science - Abstract
Clonal complex 398 livestok-associated-MRSA (CC398 LA-MRSA) clone is described as a major animal pathogen that can also colonize and infect humans. CC398 methicillin susceptible Staphylococcus aureus (CC398 MSSA) is less described. We identified 126 CC398 MSSA strains of human origin within 6380 S. aureus isolates gathered between 2009 and 2011, from the French National Reference Centre for Staphylococci. They were characterized using antimicrobial susceptibility testing, spa typing, DNA microarrays (Identibac S. aureus Genotyping ®, Alere), CC398-specific sequence PCR, ermT (encoding macrolides résistance) PCR. Fifty-three CC398 LA-MRSA collected from French pigs and veal were used as comparators, and phylogenetic relations between human CC398 MSSA and animal CC398 MRSA populations were explored on the basis of spa-typing and DNA microarrays. CC398 MSSA were able to induce a large spectrum of infections (especially skin, bloodstream, and pneumonias). The prevalence rate of this clone was high in MSSA population, i.e., 24.7% in a local prospective study on nasal colonization, and 7.5% in a national prospective study on infective endocarditis. CC398 MSSA isolates were frequently (89%) erythromycin resistant, due to the presence of the ermT gene, a gene not detected in erythromycin resistant CC398 LA-MRSA strains. Expression of staphylococcal complement inhibitor (scn) and the chemotaxis inhibitory protein (chp), was also specific to this population. The CC398 MRSA signature included also a panel of antibiotic resistance genes, especially a type IV or V cassette mec and tetM. CC398 MSSA and CC398 LA-MRSA populations were closely related based on spa-typing and DNA microarrays, with the MRSA strains forming the most derived lineage in phylogenic trees. Both MSSA and MRSA populations may come from common ancestors, which would have evolved in the settings of different selective pressures, explaining the acquisition of ermT, chp and scn for MSSA, and antibiotic resistance genes for MRSA.
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- 2013
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15. Rapid differentiation between livestock-associated and livestock-independent Staphylococcus aureus CC398 clades.
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Marc Stegger, Cindy M Liu, Jesper Larsen, Katerina Soldanova, Maliha Aziz, Tania Contente-Cuomo, Andreas Petersen, Stien Vandendriessche, Judy N Jiménez, Caterina Mammina, Alex van Belkum, Saara Salmenlinna, Frederic Laurent, Robert L Skov, Anders R Larsen, Paal S Andersen, and Lance B Price
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Medicine ,Science - Abstract
Staphylococcus aureus clonal complex 398 (CC398) isolates cluster into two distinct phylogenetic clades based on single-nucleotide polymorphisms (SNPs) revealing a basal human clade and a more derived livestock clade. The scn and tet(M) genes are strongly associated with the human and the livestock clade, respectively, due to loss and acquisition of mobile genetic elements. We present canonical single-nucleotide polymorphism (canSNP) assays that differentiate the two major host-associated S. aureus CC398 clades and a duplex PCR assay for detection of scn and tet(M). The canSNP assays correctly placed 88 S. aureus CC398 isolates from a reference collection into the human and livestock clades and the duplex PCR assay correctly identified scn and tet(M). The assays were successfully applied to a geographically diverse collection of 272 human S. aureus CC398 isolates. The simple assays described here generate signals comparable to a whole-genome phylogeny for major clade assignment and are easily integrated into S. aureus CC398 surveillance programs and epidemiological studies.
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- 2013
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16. Subpopulations of Staphylococcus aureus clonal complex 121 are associated with distinct clinical entities.
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Kevin Kurt, Jean-Philippe Rasigade, Frederic Laurent, Richard V Goering, Helena Žemličková, Ivana Machova, Marc J Struelens, Andreas E Zautner, Silva Holtfreter, Barbara Bröker, Stephen Ritchie, Sin Reaksmey, Direk Limmathurotsakul, Sharon J Peacock, Christiane Cuny, Franziska Layer, Wolfgang Witte, and Ulrich Nübel
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Medicine ,Science - Abstract
We investigated the population structure of Staphylococcus aureus clonal complex CC121 by mutation discovery at 115 genetic housekeeping loci from each of 154 isolates, sampled on five continents between 1953 and 2009. In addition, we pyro-sequenced the genomes from ten representative isolates. The genome-wide SNPs that were ascertained revealed the evolutionary history of CC121, indicating at least six major clades (A to F) within the clonal complex and dating its most recent common ancestor to the pre-antibiotic era. The toxin gene complement of CC121 isolates was correlated with their SNP-based phylogeny. Moreover, we found a highly significant association of clinical phenotypes with phylogenetic affiliations, which is unusual for S. aureus. All isolates evidently sampled from superficial infections (including staphylococcal scalded skin syndrome, bullous impetigo, exfoliative dermatitis, conjunctivitis) clustered in clade F, which included the European epidemic fusidic-acid resistant impetigo clone (EEFIC). In comparison, isolates from deep-seated infections (abscess, furuncle, pyomyositis, necrotizing pneumonia) were disseminated in several clades, but not in clade F. Our results demonstrate that phylogenetic lineages with distinct clinical properties exist within an S. aureus clonal complex, and that SNPs serve as powerful discriminatory markers, able to identify these lineages. All CC121 genomes harboured a 41-kilobase prophage that was dissimilar to S. aureus phages sequenced previously. Community-associated MRSA and MSSA from Cambodia were extremely closely related, suggesting this MRSA arose in the region.
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- 2013
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17. Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock
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Lance B. Price, Marc Stegger, Henrik Hasman, Maliha Aziz, Jesper Larsen, Paal Skytt Andersen, Talima Pearson, Andrew E. Waters, Jeffrey T. Foster, James Schupp, John Gillece, Elizabeth Driebe, Cindy M. Liu, Burkhard Springer, Irena Zdovc, Antonio Battisti, Alessia Franco, Jacek Żmudzki, Stefan Schwarz, Patrick Butaye, Eric Jouy, Constanca Pomba, M. Concepción Porrero, Raymond Ruimy, Tara C. Smith, D. Ashley Robinson, J. Scott Weese, Carmen Sofia Arriola, Fangyou Yu, Frederic Laurent, Paul Keim, Robert Skov, and Frank M. Aarestrup
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Microbiology ,QR1-502 - Abstract
ABSTRACT Since its discovery in the early 2000s, methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 398 (CC398) has become a rapidly emerging cause of human infections, most often associated with livestock exposure. We applied whole-genome sequence typing to characterize a diverse collection of CC398 isolates (n = 89), including MRSA and methicillin-susceptible S. aureus (MSSA) from animals and humans spanning 19 countries and four continents. We identified 4,238 single nucleotide polymorphisms (SNPs) among the 89 core genomes. Minimal homoplasy (consistency index = 0.9591) was detected among parsimony-informative SNPs, allowing for the generation of a highly accurate phylogenetic reconstruction of the CC398 clonal lineage. Phylogenetic analyses revealed that MSSA from humans formed the most ancestral clades. The most derived lineages were composed predominantly of livestock-associated MRSA possessing three different staphylococcal cassette chromosome mec element (SCCmec) types (IV, V, and VII-like) including nine subtypes. The human-associated isolates from the basal clades carried phages encoding human innate immune modulators that were largely missing among the livestock-associated isolates. Our results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA. The lineage appears to have undergone a rapid radiation in conjunction with the jump from humans to livestock, where it subsequently acquired tetracycline and methicillin resistance. Further analyses are required to estimate the number of independent genetic events leading to the methicillin-resistant sublineages, but the diversity of SCCmec subtypes is suggestive of strong and diverse antimicrobial selection associated with food animal production. IMPORTANCE Modern food animal production is characterized by densely concentrated animals and routine antibiotic use, which may facilitate the emergence of novel antibiotic-resistant zoonotic pathogens. Our findings strongly support the idea that livestock-associated MRSA CC398 originated as MSSA in humans. The jump of CC398 from humans to livestock was accompanied by the loss of phage-carried human virulence genes, which likely attenuated its zoonotic potential, but it was also accompanied by the acquisition of tetracycline and methicillin resistance. Our findings exemplify a bidirectional zoonotic exchange and underscore the potential public health risks of widespread antibiotic use in food animal production.
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- 2012
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18. Methicillin-resistant Staphylococcus capitis with reduced vancomycin susceptibility causes late-onset sepsis in intensive care neonates.
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Jean-Philippe Rasigade, Olivia Raulin, Jean-Charles Picaud, Charlotte Tellini, Michele Bes, Jacqueline Grando, Mohamed Ben Saïd, Olivier Claris, Jerome Etienne, Sylvestre Tigaud, and Frederic Laurent
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Medicine ,Science - Abstract
BackgroundCoagulase-negative staphylococci, mainly Staphylococcus epidermidis, are the most frequent cause of late-onset sepsis (LOS) in the neonatal intensive care unit (NICU) setting. However, recent reports indicate that methicillin-resistant, vancomycin-heteroresistant Staphylococcus capitis could emerge as a significant pathogen in the NICU. We investigated the prevalence, clonality and vancomycin susceptibility of S. capitis isolated from the blood of NICU infants and compared these data to adult patients.Methodology/principal findingsWe conducted a retrospective laboratory-based survey of positive blood cultures in NICU infants ≥ 3 days of age (n = 527) and in adult ICU patients ≥ 18 years of age (n = 1473) who were hospitalized from 2004 to 2009 in two hospital centers in Lyon, France. S. capitis was the most frequent pathogen in NICU infants, ahead of S. epidermidis (39.1% vs. 23.5% of positive blood cultures, respectively). Conversely, S. capitis was rarely found in adult ICU patients (1.0%) compared to S. epidermidis (15.3%). S. capitis bloodstream isolates were more frequently resistant to methicillin when collected from NICU infants than from adult patients (95.6% vs. 53.3%, respectively). Furthermore, we collected and characterized 53 S. capitis bloodstream isolates from NICU infants and adult patients from six distant cities. All methicillin-resistant S. capitis isolates from NICU infants were clonally related as determined by pulsed-field gel electrophoresis. These isolates harbored a type V-related staphylococcal chromosomal cassette mec element, and constantly showed either vancomycin resistance (37.5%) or heteroresistance (62.5%). Conversely, the isolates that were collected outside of the NICU were genetically diverse and displayed much lower rates of vancomycin resistance and heteroresistance (7.7% and 23.1%, respectively).Conclusions/significanceA clonal population of methicillin-resistant S. capitis strains has spread into several French NICUs. These isolates exhibit reduced susceptibility to vancomycin, which is the most widely used antimicrobial agent in the NICU setting.
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- 2012
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19. A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus.
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Stefan Monecke, Geoffrey Coombs, Anna C Shore, David C Coleman, Patrick Akpaka, Michael Borg, Henry Chow, Margaret Ip, Lutz Jatzwauk, Daniel Jonas, Kristina Kadlec, Angela Kearns, Frederic Laurent, Frances G O'Brien, Julie Pearson, Antje Ruppelt, Stefan Schwarz, Elizabeth Scicluna, Peter Slickers, Hui-Leen Tan, Stefan Weber, and Ralf Ehricht
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Medicine ,Science - Abstract
In recent years, methicillin-resistant Staphylococcus aureus (MRSA) have become a truly global challenge. In addition to the long-known healthcare-associated clones, novel strains have also emerged outside of the hospital settings, in the community as well as in livestock. The emergence and spread of virulent clones expressing Panton-Valentine leukocidin (PVL) is an additional cause for concern. In order to provide an overview of pandemic, epidemic and sporadic strains, more than 3,000 clinical and veterinary isolates of MRSA mainly from Germany, the United Kingdom, Ireland, France, Malta, Abu Dhabi, Hong Kong, Australia, Trinidad & Tobago as well as some reference strains from the United States have been genotyped by DNA microarray analysis. This technique allowed the assignment of the MRSA isolates to 34 distinct lineages which can be clearly defined based on non-mobile genes. The results were in accordance with data from multilocus sequence typing. More than 100 different strains were distinguished based on affiliation to these lineages, SCCmec type and the presence or absence of PVL. These strains are described here mainly with regard to clinically relevant antimicrobial resistance- and virulence-associated markers, but also in relation to epidemiology and geographic distribution. The findings of the study show a high level of biodiversity among MRSA, especially among strains harbouring SCCmec IV and V elements. The data also indicate a high rate of genetic recombination in MRSA involving SCC elements, bacteriophages or other mobile genetic elements and large-scale chromosomal replacements.
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- 2011
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20. Lethal Necrotizing Pneumonia Caused by an ST398 Staphylococcus aureus Strain
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Jean-Philippe Rasigade, Frederic Laurent, Philippe Hubert, François Vandenesch, and Jerome Etienne
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Community-acquired infections ,pneumonia ,bacteria ,Staphylococcus aureus ,methicillin-resistant ,Panton-Valentine leukocidin ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2010
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21. Performances of the BD MAX™ CDIFF assay for the detection of toxigenic Clostridioides difficile using Cary-Blair preserved samples
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Anne-Gaëlle, Ranc, Célia, Sentis, Jeanne, Couturier, Frédéric, Barbut, Anne, Tristan, Coralie, Buis, Kevin, Santos, Sonia, Farrah, Pascaline, Duraffourg, Francois, Vandenesch, Olivier, Dauwalder, and Fréderic, Laurent
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- 2022
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22. Paving the way for phage therapy using novel drug delivery approaches
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Thomas Briot, Camille Kolenda, Tristan Ferry, Mathieu Medina, Frederic Laurent, Gilles Leboucher, and Fabrice Pirot
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Drug Delivery Systems ,Bacteria ,Humans ,Pharmaceutical Science ,Bacteriophages ,Bacterial Infections ,Phage Therapy ,Anti-Bacterial Agents - Abstract
Bacterial resistance against antibiotics is an emergent medical issue. The development of novel therapeutic approaches is urgently needed and, in this context, bacteriophages represent a promising strategy to fight multi resistant bacteria. However, for some applications, bacteriophages cannot be used without an appropriate drug delivery system which increases their stability or provides an adequate targeting to the site of infection. This review summarizes the main application routes for bacteriophages and presents the new delivery approaches designed to increase phage's activity. Clinical successes of these formulations are also highlighted. Globally, this work paves the way for the design and optimization of nano and micro delivery systems for phage therapy.
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- 2022
23. 991. Single Center, Exploratory, Open-label Prospective Study Using the Minimally Invasive LysinDAIR Procedure (Lysin Administration During an Arthroscopic DAIR Procedure) in Patients with Suspected Relapsing Chronic Coagulase-Negative Staphylococci (CNS) Prosthetic Hip Infection (PHI)
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Tristan Ferry, Anne Conrad, Camille Kolenda, Frederic Laurent, Axel Schmidt, Cara Cassino, Sebastien Lustig, and Cécile Batailler
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Infectious Diseases ,Oncology - Abstract
Background Exebacase, a recombinant staphylococcal lysin, has: (i) reported proof-of-concept data in Phase II in S. aureus bacteremia; (ii) demonstrated antibiofilm activity in vitro against S. epidermidis; and (iii) been used as salvage therapy in 4 patients with relapsing multidrug-resistant (MDR) S. epidermidis knee prosthetic joint infection (PJI) using the LysinDAIR (arthroscopic administration of exebacase [Lysin] with Debridement and Antibiotics Implant Retention) procedure. Surgical treatment of recurrent PHIs has high risk of morbidity and loss of function. We now report our experience with the LysinDAIR procedure for PHIs. Methods We performed a single center, exploratory, open-label prospective study using LysinDAIR and suppressive antimicrobial therapy (SAT) in patients with recurrent, chronic (inoculation >3 months prior to treatment) CNS PHI. In agreement with the French Health authority, exebacase (2 to 3.5 total mg in 30-50 ml [∼0.067 – 0.075 mg/ml]) was arthroscopically administered directly into the joint. Results Three consecutive patients were included (55 to 75 years). All had previous iterative hip prosthesis exchange with a high total number of surgeries (from 6 to 10) and recent persistent or intermittent fistula (Figure). All refused hip prosthesis explantation or surgeon considered explantation too high risk for loss of function and severe post-operative complications. Patients A and B had MDR S. epidermidis; Patient B had P. aeruginosa co-infection at the time of LysinDAIR. Patient C had a prior persistent S. lugdunensis PHI and relapse on arthrocentesis; K. pneumoniae was cultured at the time of LysinDAIR. No adverse events related to exebacase or the procedure were reported. At 2 years follow up, all patients had resolution of fistula and no clinical signs of infection on SAT (tedizolid (+/- ciprofloxacin [Patient B] or cotrimoxazole [Patient C]). Description of the patients treated with the LysinDAIR procedure Local signs of infection with discharge of the three patients (A, B and C) with complex relapsing prosthetic hip infection with X-ray before the LysinDAIR procedure and at 2 years of follow-up Conclusion The LysinDAIR procedure was easy-to-perform, safe and may have therapeutic potential to facilitate the success of SAT salvage therapy for chronic relapsing CNS PJIs, and potential cure of initial chronic PJI. Randomized clinical trials evaluating the LysinDAIR procedure in these clinical situations are clearly warranted. Disclosures Tristan Ferry, MD, PhD, Contrafect: Advisor/Consultant Camille Kolenda, n/a, Contrafect: Research grant frederic Laurent, n/a, Contrafect: Research grant cara cassino, n/a, Contrafect: Employee.
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- 2022
24. Efficacy of ceftazidime-avibactam in various combinations for the treatment of experimental osteomyelitis due to Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae
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Benjamin Davido, Anne-Claude Crémieux, Isabelle Vaugier, Laure Gatin, Latifa Noussair, Laurent Massias, Frederic Laurent, Azzam Saleh-Mghir, Infection et inflammation (2I), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Raymond Poincaré [Garches], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre d’Investigation Clinique 1429 [Garches] (CIC 1429), Hôpital Raymond Poincaré [AP-HP]-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Microbiologie [Garches], Hôpital Raymond Poincaré [AP-HP], AP-HP - Hôpital Bichat - Claude Bernard [Paris], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Pfizer, Institut de Médecine et d'Epidémiologie Appliquée, IMEA, This study was supported by a grant from Pfizer., and The authors would like to thank all their colleagues at Raymond-Poincare Teaching Hospital, especially Pierre De Truchis for his unfailing support. Authors would also like to thank Beatrice Gadaleta from l'Institut de Médecine et d'Epidémiologie Appliquée (IMEA) in Paris for her logistic assistance.
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Microbiology (medical) ,Klebsiella pneumoniae ,KPC ,Infectious Diseases ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Ceftazidime-avibactam ,Pharmacology (medical) ,Osteomyelitis ,General Medicine - Abstract
International audience; Background: : Optimal treatment of carbapenemase-producing Enterobacterales (CPE) bone infections is poorly defined. This study evaluated the efficacy of the novel beta-lactam-beta-lactamase inhibitor—ceftazidime-avibactam (CAZ-AVI)—with different antibiotic combinations in an experimental model of CPE osteomyelitis. Methods: : KPC-99YC is a clinical strain of Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae with intermediate susceptibility to meropenem (MIC 4 mg/L), gentamicin (MIC 0.25 mg/L), colistin (MIC 0.25 mg/L), fosfomycin (MIC 4 mg/L) and ceftazidime-avibactam (MIC 1 mg/L). Time-kill curves were performed at 4x MIC. Osteomyelitis was induced in rabbits by tibial injection of 2×108 CFU of KPC-99YC. Six groups started treatment 14 days later for 7 days: control, colistin, CAZ-AVI, CAZ-AVI plus gentamicin, CAZ-AVI plus colistin and CAZ-AVI plus fosfomycin. Antibiotic dosages were selected to simulate plasma concentrations obtained in humans. Treatment was evaluated according to bone cultures quantified in log10 CFU. Results: : In vitro, CAZ-AVI plus colistin or gentamicin were rapidly bactericidal in contrast with CAZ-AVI plus fosfomycin. In vivo, compared with controls, colistin alone (P = 0.045) and CAZ-AVI alone or in combination significantly lowered bone bacterial counts (P < 0.001). Bone sterilisation was achieved in 67% and 100% of animals with combinations of CAZ-AVI plus colistin or gentamicin (P = 0.001 and P < 0.001, respectively) whereas other treatments were no different from controls. CAZ-AVI plus gentamicin provided greater bone bacterial reduction than CAZ-AVI plus colistin (P = 0.033). No CAZ-AVI-resistant strains emerged in treated rabbits, regardless of combination. Conclusions: : CAZ-AVI plus gentamicin was the best effective combination therapy. Combinations with CAZ-AVI appear to be a promising treatment of KPC-producing Klebsiella pneumoniae osteomyelitis.
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- 2022
25. Personalized bacteriophage therapy to treat pandrug-resistant spinal P. aeruginosa infection
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Tristan FERRY, Camille KOLENDA, Frederic LAURENT, Gilles LEBOUCHER, Maya Merabischvilli, Sarah DJEBARA, Claude-Alexandre GUSTAVE, Thomas Perpoint, Cédric Barrey, Jean-Paul Pirnay, and Gregory Resch
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A pandrug-resistant P. aeruginosa spinal abscess was treated with surgery and personalized phage therapy that was added to cefiderocol and colistin. After a 2-stage approach, despite bacterial persistence with expression of small colony variants, the patient cured with local and intravenous injections of purified bacteriophages as part of the treatment.
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- 2022
26. Potential of training of anti-Staphylococcus aureus therapeutic phages against Staphylococcus epidermidis multidrug-resistant isolates is restricted by inter- and intra-sequence type specificity
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Camille Kolenda, Mélanie Bonhomme, Mathieu Medina, Mateo Pouilly, Clara Rousseau, Emma Troesch, Patricia Martins-Simoes, Marc Stegger, Paul O. Verhoeven, Floriane Laumay, and Frédéric Laurent
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Staphylococcus aureus ,Staphylococcus epidermidis ,bacteriophages ,phage training ,host range extension ,adaptation ,Microbiology ,QR1-502 - Abstract
ABSTRACT Phage therapy appears to be a promising approach to tackle multidrug-resistant bacteria, including staphylococci. However, most anti-staphylococcal phages have been characterized in Staphylococcus aureus, while a limited number of studies investigated phage activity against S. epidermidis. We studied the potential of phage training to extend the host range of two types of anti-S. aureus phages against S. epidermidis isolates. The Appelmans protocol was applied to a mixture of Kayvirus and a mixture of Silviavirus phages repeatedly exposed to seven S. epidermidis strains representative of nosocomial-associated sequence types (ST), including the world-wide disseminated ST2. We observed increased activity only for the Kayvirus mixture against two of these strains (ST2 or ST35). Phage subpopulations isolated from the training mixture using these two strains (five/strain) exhibited different evolved phenotypes, active only against their isolation strain or strains of the same ST. Of note, 16/47 ST2 strains were susceptible to one of the groups of trained phages. A comparative genomic analysis of ancestral and trained phage genomes, conducted to identify potential bacterial determinants of such specific activity, found numerous recombination events between two of the three ancestors. However, a small number of trained phage genes had nucleotide sequence modifications impacting the corresponding protein compared to ancestral phages, two to four of them per phage genome being specific of each group of phage subpopulations exhibiting different host range. The results suggest that anti-S. aureus phages can be adapted to S. epidermidis isolates but with inter- and intra-ST specificity.ImportanceS. epidermidis is increasingly recognized as a threat for public health. Its clinical importance is notably related to multidrug resistance. Phage therapy is one of the most promising alternative therapeutic strategies to antibiotics. Nonetheless, only very few phages active against this bacterial species have been described. In the present study, we showed that phage training can be used to extend the host range of polyvalent Kayvirus phages within the Staphylococcus genera to include S. epidermidis species. In the context of rapid development of phage therapy, in vitro forced adaptation of previously characterized phages could be an appealing alternative to fastidious repeated isolation of new phages to improve the therapeutic potential of a phage collection.
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- 2024
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27. Emergence of methicillin resistance predates the clinical use of antibiotics
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Jesper Larsen, Claire L. Raisen, Xiaoliang Ba, Nicholas J. Sadgrove, Guillermo F. Padilla-González, Monique S. J. Simmonds, Igor Loncaric, Heidrun Kerschner, Petra Apfalter, Rainer Hartl, Ariane Deplano, Stien Vandendriessche, Barbora Černá Bolfíková, Pavel Hulva, Maiken C. Arendrup, Rasmus K. Hare, Céline Barnadas, Marc Stegger, Raphael N. Sieber, Robert L. Skov, Andreas Petersen, Øystein Angen, Sophie L. Rasmussen, Carmen Espinosa-Gongora, Frank M. Aarestrup, Laura J. Lindholm, Suvi M. Nykäsenoja, Frederic Laurent, Karsten Becker, Birgit Walther, Corinna Kehrenberg, Christiane Cuny, Franziska Layer, Guido Werner, Wolfgang Witte, Ivonne Stamm, Paolo Moroni, Hannah J. Jørgensen, Hermínia de Lencastre, Emilia Cercenado, Fernando García-Garrote, Stefan Börjesson, Sara Hæggman, Vincent Perreten, Christopher J. Teale, Andrew S. Waller, Bruno Pichon, Martin D. Curran, Matthew J. Ellington, John J. Welch, Sharon J. Peacock, David J. Seilly, Fiona J. E. Morgan, Julian Parkhill, Nazreen F. Hadjirin, Jodi A. Lindsay, Matthew T. G. Holden, Giles F. Edwards, Geoffrey Foster, Gavin K. Paterson, Xavier Didelot, Mark A. Holmes, Ewan M. Harrison, Anders R. Larsen, Larsen, Jesper [0000-0003-0582-0457], Ba, Xiaoliang [0000-0002-3882-3585], Padilla-González, Guillermo F [0000-0002-8300-6891], Černá Bolfíková, Barbora [0000-0001-8059-4889], Skov, Robert L [0000-0002-6079-5381], Rasmussen, Sophie L [0000-0002-2975-678X], Espinosa-Gongora, Carmen [0000-0002-9536-0548], Aarestrup, Frank M [0000-0002-7116-2723], Becker, Karsten [0000-0002-6391-1341], Layer, Franziska [0000-0002-4613-6478], Moroni, Paolo [0000-0002-0974-3084], Jørgensen, Hannah J [0000-0002-1788-9219], de Lencastre, Hermínia [0000-0001-6816-8932], Cercenado, Emilia [0000-0002-5279-3773], Börjesson, Stefan [0000-0003-2219-2659], Waller, Andrew S [0000-0002-7111-9549], Welch, John [0000-0001-7049-7129], Peacock, Sharon [0000-0002-1718-2782], Morgan, Fiona [0000-0003-0583-7996], Parkhill, Julian [0000-0002-7069-5958], Holden, Matthew TG [0000-0002-4958-2166], Foster, Geoffrey [0000-0002-5527-758X], Paterson, Gavin K [0000-0002-1880-0095], Didelot, Xavier [0000-0003-1885-500X], Holmes, Mark [0000-0002-5454-1625], Harrison, Ewan [0000-0003-2720-0507], Apollo - University of Cambridge Repository, Peacock, Sharon J [0000-0002-1718-2782], Holmes, Mark A [0000-0002-5454-1625], Harrison, Ewan M [0000-0003-2720-0507], University of St Andrews. School of Medicine, University of St Andrews. Biomedical Sciences Research Complex, University of St Andrews. St Andrews Bioinformatics Unit, and University of St Andrews. Infection and Global Health Division
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Denmark ,Geographic Mapping ,Methicillin Resistance/genetics ,Antimicrobial resistance ,Bacterial evolution ,Penicillins/biosynthesis ,Phylogeny ,beta-Lactams/metabolism ,Multidisciplinary ,630 Agriculture ,article ,QR Microbiology ,Anti-Bacterial Agents ,Europe ,Hedgehogs ,Hedgehogs/metabolism ,Methicillin-Resistant Staphylococcus aureus ,631/326/41/2529 ,45/22 ,45/23 ,101/58 ,Anti-Bacterial Agents/history ,Penicillins ,beta-Lactams ,Selection, Genetic/genetics ,Evolution, Molecular ,SDG 3 - Good Health and Well-being ,631/92/349/977 ,631/158/1745 ,Animals ,Humans ,One Health ,Selection, Genetic ,SDG 2 - Zero Hunger ,MCC ,Infectious-disease epidemiology ,QL ,Arthrodermataceae/genetics ,Arthrodermataceae ,DAS ,500 Naturwissenschaften und Mathematik::570 Biowissenschaften ,Biologie::570 Biowissenschaften ,Biologie ,History, 20th Century ,QR ,631/326/41/1470 ,631/326/22/1434 ,570 Life sciences ,biology ,Methicillin Resistance ,Methicillin-Resistant Staphylococcus aureus/genetics ,New Zealand - Abstract
X.D. was funded by a grant from the National Institute for Health Research (NIHR) Health Protection Research Unit in Genomics and Enabling Data (no. NIHR200892). M.A.H. was supported by grants from the Medical Research Council (nos. G1001787/1, MR/N002660/1 and MR/P007201/1) and the Economic and Social Research Council (no. ES/S000186/1). E.M.H. was supported by a UK Research and Innovation (UKRI) Fellowship (no. MR/S00291X/1). The discovery of antibiotics more than 80 years ago has led to considerable improvements in human and animal health. Although antibiotic resistance in environmental bacteria is ancient, resistance in human pathogens is thought to be a modern phenomenon that is driven by the clinical use of antibiotics1. Here we show that particular lineages of methicillin-resistant Staphylococcus aureus—a notorious human pathogen—appeared in European hedgehogs in the pre-antibiotic era. Subsequently, these lineages spread within the local hedgehog populations and between hedgehogs and secondary hosts, including livestock and humans. We also demonstrate that the hedgehog dermatophyte Trichophyton erinacei produces two β-lactam antibiotics that provide a natural selective environment in which methicillin-resistant S. aureus isolates have an advantage over susceptible isolates. Together, these results suggest that methicillin resistance emerged in the pre-antibiotic era as a co-evolutionary adaptation of S. aureus to the colonization of dermatophyte-infected hedgehogs. The evolution of clinically relevant antibiotic-resistance genes in wild animals and the connectivity of natural, agricultural and human ecosystems demonstrate that the use of a One Health approach is critical for our understanding and management of antibiotic resistance, which is one of the biggest threats to global health, food security and development. Publisher PDF
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- 2022
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28. Staphylococcus succinus Infective Endocarditis, France
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Louise Ruffier d’Epenoux, Erwan Fayoux, Frédéric Laurent, Pascale Bémer, Raphaël Lecomte, Thierry Le Tourneau, Aurélie Guillouzouic, and Stéphane Corvec
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Staphylococcus succinus ,infective endocarditis ,antimicrobial resistance ,mec gene ,penicillin-binding proteins ,France ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Infective endocarditis is a rare condition in humans and is associated with high illness and death rates. We describe a case of infective endocarditis caused by Staphylococcus succinus bacteria in France. We used several techniques for susceptibility testing for this case to determine the oxacillin profile.
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- 2024
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29. Identification and Characterization of Staphylococcus delphini Internalization Pathway in Nonprofessional Phagocytic Cells
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Yousef, Maali, Alan, Diot, Patrícia, Martins-Simões, Michele, Bes, Daniel, Bouvard, François, Vandenesch, Paul O, Verhoeven, Frederic, Laurent, and Sophie, Trouillet-Assant
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Phagocytes ,Cellular Microbiology: Pathogen-Host Cell Molecular Interactions ,Integrin beta1 ,Staphylococcus ,Bacterial Adhesion ,Cell Line ,Fibronectins ,Mice ,Bacterial Proteins ,Cell Wall ,Animals ,Humans ,Adhesins, Bacterial ,Carrier Proteins - Abstract
The intracellular lifestyle of bacteria is widely acknowledged to be an important mechanism in chronic and recurring infection. Among the Staphylococcus genus, only Staphylococcus aureus and Staphylococcus pseudintermedius have been clearly identified as intracellular in nonprofessional phagocytic cells (NPPCs), for which the mechanism is mainly fibronectin-binding dependent. Here, we used bioinformatics tools to search for possible new fibronectin-binding proteins (FnBP-like) in other Staphylococcus species. We found a protein in Staphylococcus delphini called Staphylococcus delphini surface protein Y (SdsY). This protein shares 68% identity with the Staphylococcus pseudintermedius surface protein D (SpsD), 36% identity with S. aureus FnBPA, and 39% identity with S. aureus FnBPB. The SdsY protein possesses the typical structure of FnBP-like proteins, including an N-terminal signal sequence, an A domain, a characteristic repeated pattern, and an LPXTG cell wall anchor motif. The level of adhesion to immobilized fibronectin was significantly higher in all S. delphini strains tested than in the fibronectin-binding-deficient S. aureus DU5883 strain. By using a model of human osteoblast infection, the level of internalization of all strains tested was significantly higher than with the invasive-incompetent S. aureus DU5883. These findings were confirmed by phenotype restoration after transformation of DU5883 by a plasmid expression vector encoding the SdsY repeats. Additionally, using fibronectin-depleted serum and murine osteoblast cell lines deficient for the β(1) integrin, the involvement of fibronectin and β(1) integrin was demonstrated in S. delphini internalization. The present study demonstrates that additional staphylococcal species are able to invade NPPCs and proposes a method to identify FnBP-like proteins.
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- 2020
30. Molecular characterization and antimicrobial resistance of nasal Staphylococcus aureus in the community of Kabul
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Haji Mohammad Naimi, Anne Tristan, Michèle Bes, François Vandenesch, Qand Agha Nazari, Frédéric Laurent, and Céline Dupieux
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Nasal carriage ,MRSA clones ,Antibiotic resistance ,Molecular typing ,Kabul ,Microbiology ,QR1-502 - Abstract
ABSTRACT: Objectives: This study aimed to investigate the prevalence and molecular characteristics of community methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage among students at Kabul University. Methods: Nasal swabs were collected from anterior nares of 150 healthy non-medical students at Kabul University. Antimicrobial susceptibility testing was performed on all S. aureus isolates, and all detected MRSA isolates were then confirmed by mecA/mecC polymerase chain reaction and characterized using DNA microarray. Results: A total of 50 S. aureus strains were isolated from the anterior nares of the 150 participants. The prevalence of S. aureus and MRSA nasal carriage among Kabul students was 33.3% and 12.7%, respectively. Seven (36.8%) MRSA isolates and 8 (25.8%) methicillin-susceptible S. aureus (MSSA) isolates were multidrug-resistant (i.e. resistant to at least three different antimicrobials tested). All MRSA isolates (n = 19) were susceptible to linezolid, rifampicin, and fusidic acid. Seven MRSA clones, belonging to four clonal complexes (CCs), were identified. The most commonly identified clone was CC22-MRSA-IV TSST-1-positive, which accounted for 63.2% (12/19) of MRSA isolates. SCCmec typing showed that most MRSA strains harboured SCCmec type IV (94.7%). Thirteen (68.4%) MRSA isolates carried the TSST-1 and 5 (26.3%) PVL genes. Conclusion: Our findings revealed the relatively high prevalence of MRSA nasal carriers in the community in Kabul, with the predominance of the CC22-MRSA-IV TSST-1-positive clone and frequent multidrug resistance among these isolates.
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- 2023
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31. 322. Evaluation of the BioFire® Bone and Joint Infection (BJI) Panel for the Detection of Microorganisms and Antimicrobial Resistance Genes in Synovial Fluid Specimens
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Corrin Graue, Bryan H Schmitt, Amy Waggoner, Frederic Laurent, Lelia Abad, Thomas Bauer, Irving Mazariegos, Joan-Miquel Balada-Llasat, Jarid Horn, Donna Wolk, Alexa Jefferis, Mirjam Hermans, Irma Verhoofstad, Susan Butler-Wu, Minette Umali-Wilcox, Caitlin N Murphy, Barbara J Cabrera, Jaime Esteban, Alicia Macias-Valcayo, David Craft, Benjamin von Bredow, Amy Leber, Kathy Everhart, Jennifer Dien Bard, Javier Mestas, Judy Daly, Rebecca Barr, Bart Kensinger, Benedicte Pons, and Corinne Jay
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business.industry ,Microorganism ,medicine.medical_treatment ,Arthrocentesis ,Knee Joint ,Pathogenicity ,Microbiology ,AcademicSubjects/MED00290 ,Infectious Diseases ,Oncology ,Bsnd gene ,Poster Abstracts ,Antimicrobial resistance genes ,Synovial fluid ,Medicine ,Anaerobic bacteria ,business - Abstract
Background Bone and Joint Infections (BJIs) present with non-specific symptoms that may include pain, swelling, and fever and are associated with high morbidity and significant risk of mortality. BJIs can be caused by a variety of bacteria and fungi, including anaerobes and microorganisms that can be challenging to culture or identify by traditional microbiological methods. Clinicians primarily rely on culture to identify the pathogen(s) responsible for infection. The BioFire® Bone and Joint Infection (BJI) Panel (BioFire Diagnostics, Salt Lake City, UT) is designed to detect 15 gram-positive bacteria (including seven anaerobes), 14 gram-negative bacteria (including one anaerobe), two yeast, and eight antimicrobial resistance (AMR) genes from synovial fluid specimens in about an hour. The objective of this study was to evaluate the performance of an Investigational Use Only (IUO) version of the BioFire BJI Panel compared to various reference methods. Methods Remnant synovial fluid specimens, which were collected for routine clinical care at 13 study sites in the US and Europe, underwent testing using an IUO version of the BioFire BJI Panel. Performance of this test was determined by comparison to Standard of Care (SoC) consisting of bacterial culture performed at each study site according to their routine procedures. Results A total of 1544 synovial fluid specimens were collected and tested with the BioFire BJI Panel. The majority of specimens were from knee joints (77.9%) and arthrocentesis (79.4%) was the most common collection method. Compared to SoC culture, overall sensitivity was 90.2% and specificity was 99.8%. The BioFire BJI Panel yielded a total of 268 Detected results, whereas SoC yielded a total of 215 positive results for on-panel analytes. Conclusion The BioFire BJI Panel is a sensitive, specific, and robust test for rapid detection of a wide range of analytes in synovial fluid specimens. The number of microorganisms and resistance genes included in the BioFire BJI Panel, together with a reduced time-to-result and increased diagnostic yield compared to culture, is expected to aid in the timely diagnosis and appropriate management of BJIs. Disclosures Benjamin von Bredow, PhD, BioFire (Grant/Research Support) Jennifer Dien Bard, PhD, BioFire Diagnostic (Consultant, Scientific Research Study Investigator) Bart Kensinger, PhD, BioFire Diagnostics (Employee) Benedicte Pons, PhD, bioMerieux SA (Employee) Corinne Jay, PhD, bioMerieux SA (Employee)
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- 2020
32. Salvage Debridement, Antibiotics and Implant Retention ('DAIR') With Local Injection of a Selected Cocktail of Bacteriophages: Is It an Option for an Elderly Patient With Relapsing Staphylococcus aureus Prosthetic-Joint Infection?
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Cyril Courtin, Tristan Ferry, Jérôme JOSSE, Yannick HERRY, Mikhail Dziadzko, Frederic Laurent, Stanislas GUNST, Florent Valour, Cédric Dananché, Jean-luc Besse, Florence ADER, Camille Kolenda, Pathogénie des Staphylocoques – Staphylococcal Pathogenesis (StaPath), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Service de Maladies Infectieuses et Tropicales [Hôpital de la Croix-Rousse - HCL], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Centre de référence des infections ostéo-articulaires complexes Rhône Alpes Auvergne, Hospices Civils de Lyon (HCL), Service Pharmacie, Groupement Hospitalier Nord, Hospices Civils de Lyon, Lyon, France, parent, Pherecydes Pharma, Service de Chirurgie Orthopédique [Centre Albert Trillat], Centre Albert Trillat [Hôpital de la Croix-Rousse - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL)-Hôpital de la Croix-Rousse [CHU - HCL], Pathogenèse des légionelles- Legionella pathogenesis (LegioPath), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,medicine.medical_specialty ,[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging ,medicine.drug_class ,medicine.medical_treatment ,030106 microbiology ,Antibiotics ,medicine.disease_cause ,Clinical success ,suppressive therapy ,03 medical and health sciences ,bacteriophage ,medicine ,Elderly patient ,DAIR ,Debridement ,business.industry ,prosthetic-joint infection ,Prosthetic joint infection ,S. aureus ,3. Good health ,Surgery ,030104 developmental biology ,Infectious Diseases ,Oncology ,Staphylococcus aureus ,Implant ,Local injection ,business - Abstract
[PubMed Central:\hrefhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240628PMC6240628] [DOI:\hrefhttps://dx.doi.org/10.1093/ofid/ofy26910.1093/ofid/ofy269] [PubMed:\hrefhttps://www.ncbi.nlm.nih.gov/pubmed/3029248130292481]; International audience; Local injection of a bacteriophages mix during debridement, antibiotics and implant retention ("DAIR") was performed to treat a relapsing Staphylococcus aureus chronic prosthetic joint infection (PJI). This salvage treatment was safe and associated with a clinical success. Scientific evaluation of the potential clinical benefit of bacteriophages as antibiofilm treatment in PJI is now feasible and required.
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- 2018
33. A Meta-Analysis of Outcomes After In Situ Reconstructions for Aortic Graft Infection
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Michel Batt, Matthieu Revest, Frederic Laurent, Christian Chidiac, Amandine Coffy, Jocelyne Caillon, Jean Pierre Daures, Brigitte Calvet, Patrick Feugier, Eric Senneville, Fabrice Camou, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Aide à la Décision pour une Médecine Personnalisé - Laboratoire de Biostatistique, Epidémiologie et Recherche Clinique - EA 2415 (AIDMP), Université Montpellier 1 (UM1)-Université de Montpellier (UM), Unité de Chirurgie Vasculaire, CHU Lyon, Hôpital Saint-André, Département des Maladies Infectieuses [CH Tourcoing] (Hôpital Gustave Dron), Centre Hospitalier de Tourcoing-Centre Hospitalier Gustave Dron [Tourcoing], Service de bactériologie et hygiène hospitalière [Nantes], Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), CH Béziers, Pathogénie des Staphylocoques – Staphylococcal Pathogenesis (StaPath), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS), Centre Hospitalier Gustave Dron [Tourcoing]-Centre Hospitalier de Tourcoing, Centre International de Recherche en Infectiologie (CIRI), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
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Reoperation ,medicine.medical_specialty ,Prosthesis-Related Infections ,reconstruction ,[SDV]Life Sciences [q-bio] ,Treatment outcome ,030204 cardiovascular system & hematology ,Prosthesis Design ,Blood Vessel Prosthesis Implantation ,03 medical and health sciences ,0302 clinical medicine ,Blood vessel prosthesis ,Humans ,Medicine ,Prosthesis design ,030212 general & internal medicine ,Prosthesis-Related Infection ,Aortic graft ,business.industry ,in situ ,infection ,Blood Vessel Prosthesis ,3. Good health ,Surgery ,Treatment Outcome ,surgical procedures, operative ,graft ,cardiovascular system ,Cardiology and Cardiovascular Medicine ,business ,aortic - Abstract
Objective: To confirm the advantage of in situ reconstruction (ISR) over extra-anatomic reconstruction (EAR) for aortic graft infection and determine the most appropriate conduit including autogenous veins, cryopreserved allografts, and synthetic prosthesis (standard, rifampicin of silver polyesters). Methods: A meta-analysis was conducted with rate of mortality, graft occlusion, amputation, and reinfection. A meta-regression was performed with 4 factors: patients’ age, presence of prosthetic-duodenal fistula (PDF), virulent organisms, or nonvirulent organisms. Results: In situ reconstruction over EAR seems to favor all events. For the 5 conduits used for ISR, according to operative mortality, age of the patients looks to have a positive correlation only for silver polyester and no conduit present any advantage in the presence of PDF. Reinfection seems to be not significantly different for the 5 conduits, and only autogenous veins appear to have a positive correlation with infecting organisms. Conclusion: In situ reconstruction may be considered as first-line treatment. Our results suggest that silver polyesters appear to be most appropriate for older patients, and in order to limit reinfection, autogenous veins are probably the most suitable conduit.
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- 2018
34. Chronic and severe prosthetic joint infection complicated by amyloid A amyloidosis with renal and bladder impairment
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Cyril Courtin, Tristan Ferry, Mikhail Dziadzko, Francois Vandenesch, Frederic Laurent, Stanislas GUNST, Florent Valour, Florence ADER, Marc Janier, Service de Maladies Infectieuses et Tropicales [Hôpital de la Croix-Rousse - HCL], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Service de Chirurgie Orthopédique [Centre Albert Trillat], Centre Albert Trillat [Hôpital de la Croix-Rousse - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL)-Hôpital de la Croix-Rousse [CHU - HCL], Pathogénie des Staphylocoques – Staphylococcal Pathogenesis (StaPath), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
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medicine.medical_specialty ,Prosthesis-Related Infections ,Images In… ,medicine.medical_treatment ,030232 urology & nephrology ,Bone Neoplasms ,medicine.disease_cause ,Prosthesis ,Amputation, Surgical ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Synovial fluid ,Humans ,Femur ,Medical history ,030212 general & internal medicine ,Renal Insufficiency ,ComputingMilieux_MISCELLANEOUS ,Aged ,Hematuria ,Serum Amyloid A Protein ,business.industry ,Amyloidosis ,Urinary Bladder Diseases ,General Medicine ,bone and joint infections ,medicine.disease ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Surgery ,Superinfection ,Orthopedic surgery ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Female ,Methicillin Resistance ,business ,Knee Prosthesis ,Bandage ,orthopaedics - Abstract
A 66-year-old woman presented with renal failure and purulent discharge associated with a chronic prosthetic joint infection (PJI) of the left knee. Her medical history consisted of an untreated chronic hepatitis B and recurrent giant-cell tumour of bone revealed by spontaneous fractures 20 years previously. Multiple tumour resections led to knee prosthesis implantation in 1992 with no tumour relapse thereafter. The patient experienced a methicillin-susceptible Staphylococcus aureus chronic PJI in the following months that required two-stage prosthesis replacement and a prolonged antibiotherapy. A clinical suspicion of superinfection was confirmed in 2002 by a puncture of the synovial fluid that found a methicillin-resistant Staphylococcus epidermidis . The patient declined both surgical and medical treatment and was lost to follow-up. She subsequently presented in 2015 with partial impotence, no flexion of the left knee and multiple fistulae with purulent discharge (figure 1A) that had evolved for years and that had been treated with a self-made bandage. X-ray (figure 1B) and CT scan (figure 1C) found extensive periostea reaction of the femur, bone destruction and prosthesis loosening. Lab results found …
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- 2018
35. Correction of Linezolid-Induced Myelotoxicity After Switch to Tedizolid in a Patient Requiring Suppressive Antimicrobial Therapy for Multidrug-Resistant Staphylococcus epidermidis Prosthetic-Joint Infection
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Cyril Courtin, Tristan Ferry, Jérôme JOSSE, Yannick HERRY, Mikhail Dziadzko, Frederic Laurent, Stanislas GUNST, Florent Valour, Florence ADER, Camille Kolenda, Pathogénie des Staphylocoques – Staphylococcal Pathogenesis (StaPath), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Service de Maladies Infectieuses et Tropicales [Hôpital de la Croix-Rousse - HCL], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Centre interrégional de référence Rhône-Alpes - Auvergne des infections ostéo-articulaires complexes, Pathogenèse des légionelles- Legionella pathogenesis (LegioPath), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,Blood transfusion ,Anemia ,[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging ,medicine.medical_treatment ,030106 microbiology ,Hematocrit ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Medicine ,030212 general & internal medicine ,medicine.diagnostic_test ,business.industry ,medicine.disease ,3. Good health ,Infectious Diseases ,Oncology ,chemistry ,Concomitant ,Anesthesia ,Linezolid ,Vancomycin ,Tedizolid ,Daptomycin ,business ,medicine.drug - Abstract
[PubMed Central:\hrefhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198638PMC6198638] [DOI:\hrefhttps://dx.doi.org/10.1093/ofid/ofy24610.1093/ofid/ofy246] [PubMed:\hrefhttps://www.ncbi.nlm.nih.gov/pubmed/2937035029370350]; International audience; A 71-year-old man (85 kg) has a past history of vitiligo, ischemic myocardiopathy, and bilateral knee arthroplasties. A 1-stage exchange of the right prosthetic-joint infection (PJI) was done in 2016 for a mechanical prosthetic loosening. A massive constrained prosthetic joint was used to compensate for the bone loss (Supplementary Figure S1A). Iterative postoperative dislocations were followed by occurrence of a fistula in January 2017 and prosthetic loosening (Supplementary Figure S1B) without any pain. Because it was impossible to imagine a 2-stage exchange, a debridement and implant retention (DAIR) procedure followed by suppressive antimicrobial therapy was proposed. Daptomycin (700 mg/day) and ceftaroline (1200 mg/day) were prescribed after the surgery. A multidrug-resistant Staphylococcus epidermidis, which is only susceptible to daptomycin, vancomycin, fosfomycin, and linezolid, was found in culture from all operative samples. After 6 weeks of intravenous antimicrobial therapy, 600 mg of linezolid bid was prescribed in August 2017. Concomitant medications were ramipril, bisoprolol, furosemide, and aspirin. Under therapy, the patient experienced a progressive decrease of hemoglobin and hematocrit (without decrease of white blood cells or platelets). Five months after linezolid introduction, the patient developed asthenia related to anemia, with a decrease of hemoglobin to 65 mg/dL, and without leucopenia or thrombocytopenia (Figure 1). The patient did not take any treatment with potential bone marrow toxicity, except linezolid. The patient has no other adverse drug reactions. A blood transfusion (2 bags) was performed, which led to an immediate increase of the hemoglobin level to 84 mg/dL, and linezolid was switched to 200 mg of tedizolid once a day. In May 2018, 9 months after the DAIR surgery and 4 months after the switch, the patient was perfectly fine, walked despite rupture of the right knee extensor apparatus (video S2), without any pain, without any local signs of relapse (Supplementary Figure S1C), without clinical signs of neuropathy, and he experienced a continuous increase of the hemoglobin to 14 mg/dL under tedizolid therapy. No other treatment was changed or introduced.
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- 2018
36. Antimicrobial Susceptibility and Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Tunisia: Results of a Multicenter Study
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Senda Mezghani, Maalej, primary, Jihene Jdidi, Trabelsi, additional, Gustave, Claude-alexandre, additional, Ilhem, Boutiba, additional, Maha, Mastouri, additional, Sophia, Besbes, additional, Farouk, Barguellil, additional, Frederic, Laurent, additional, and Adnene, Hammami, additional
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- 2019
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37. Routine Whole-Genome Sequencing for Outbreak Investigations of
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Geraldine, Durand, Fabien, Javerliat, Michèle, Bes, Jean-Baptiste, Veyrieras, Ghislaine, Guigon, Nathalie, Mugnier, Stéphane, Schicklin, Gaël, Kaneko, Emmanuelle, Santiago-Allexant, Coralie, Bouchiat, Patrícia, Martins-Simões, Frederic, Laurent, Alex, Van Belkum, François, Vandenesch, and Anne, Tristan
- Subjects
Staphylococcus aureus ,whole-genome sequencing ,bioinformatics pipeline ,de novo assembly ,Microbiology ,national reference center ,Original Research ,outbreak investigation - Abstract
The French National Reference Center for Staphylococci currently uses DNA arrays and spa typing for the initial epidemiological characterization of Staphylococcus aureus strains. We here describe the use of whole-genome sequencing (WGS) to investigate retrospectively four distinct and virulent S. aureus lineages [clonal complexes (CCs): CC1, CC5, CC8, CC30] involved in hospital and community outbreaks or sporadic infections in France. We used a WGS bioinformatics pipeline based on de novo assembly (reference-free approach), single nucleotide polymorphism analysis, and on the inclusion of epidemiological markers. We examined the phylogeographic diversity of the French dominant hospital-acquired CC8-MRSA (methicillin-resistant S. aureus) Lyon clone through WGS analysis which did not demonstrate evidence of large-scale geographic clustering. We analyzed sporadic cases along with two outbreaks of a CC1-MSSA (methicillin-susceptible S. aureus) clone containing the Panton–Valentine leukocidin (PVL) and results showed that two sporadic cases were closely related. We investigated an outbreak of PVL-positive CC30-MSSA in a school environment and were able to reconstruct the transmission history between eight families. We explored different outbreaks among newborns due to the CC5-MRSA Geraldine clone and we found evidence of an unsuspected link between two otherwise distinct outbreaks. Here, WGS provides the resolving power to disprove transmission events indicated by conventional methods (same sequence type, spa type, toxin profile, and antibiotic resistance profile) and, most importantly, WGS can reveal unsuspected transmission events. Therefore, WGS allows to better describe and understand outbreaks and (inter-)national dissemination of S. aureus lineages. Our findings underscore the importance of adding WGS for (inter-)national surveillance of infections caused by virulent clones of S. aureus but also substantiate the fact that technological optimization at the bioinformatics level is still urgently needed for routine use. However, the greatest limitation of WGS analysis is the completeness and the correctness of the reference database being used and the conversion of floods of data into actionable results. The WGS bioinformatics pipeline (EpiSeqTM) we used here can easily generate a uniform database and associated metadata for epidemiological applications.
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- 2017
38. Disinfection of incubators in neonatal intensive care units: impact of steam pulverization on bacterial colonization
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Marion Reboux, Marie Chavignon, Anne Tristan, Franck Plaisant, Frédéric Laurent, and Marine Butin
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Neonatal intensive care units ,Neonatal incubators ,Bacterial contamination ,Disinfection ,Steam pulverization ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background In neonatal intensive care units (NICUs), neonates requiring medical care after birth, including very vulnerable preterm infants, are housed in incubators. Previous studies have reported that the standard chemical disinfection measures used to disinfect these incubators are insufficient to eradicate contaminating bacteria, leading to a worrying infectious risk for preterm neonates. This study aimed to evaluate the efficacy of a disinfection method based on steam pulverization to eradicate the persistent bacterial contamination in such incubators. Methods In a tertiary NICU, 20 incubators were monitored qualitatively for bacterial contamination at five different sites (the rubber grommet, the left door handles, the temperature adjustment button, the mattress and the scale) using a culture method at three times: before and after steam pulverization then 24 h after turning on and housing a new neonate. Clinical data of neonates housed in each incubator were retrieved from the medical records to identify potential occurrence of late onset sepsis (LOS). Results Just after steam pulverization, only two incubators were free from bacteria. Before disinfection 87% of all the samples were contaminated compared to 61% after disinfection. After 24 h, the proportion of contaminated samples reached 85%. Mattresses and scales were the most frequently contaminated incubator sites with respectively 90% and 80% positive samples after disinfection compared to 100% and 90% before disinfection. Coagulase-negative staphylococci, Enterococcus, Enterobacteria and Bacillus resisted disinfection and were identified on respectively 90%, 20%, 5% and 45% of incubators just after disinfection. Three preterm neonates developed LOS after being housed in a disinfected incubator but the bacterial species involved have not been identified in their incubator after disinfection. In two cases, the bacterium had been isolated from the mattress 24 h after housing the infected patient. Conclusion Steam pulverization is not sufficient to eradicate bacterial contamination of incubators. These results highlight the urgent need for an effective disinfection method, especially for mattresses that are in constant contact with patients. In parallel, new incubator designs and mattress protections must be developed.
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- 2023
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39. Evaluation of ceftolozane-tazobactam susceptibility on a French nationwide collection of Enterobacterales
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Agnès B. Jousset, Sandrine Bernabeu, Cécile Emeraud, Rémy A. Bonnin, Alexandra Lomont, Jean Ralph Zahar, Audrey Merens, Christophe Isnard, Nathalie Soismier, Eric Farfour, Vincent Fihman, Nicolas Yin, Olivier Barraud, Hervé Jacquier, Anne-Gaëlle Ranc, Frédéric Laurent, Stéphane Corvec, Louise Ruffier d'Epenoux, Emmanuelle Bille, Nicolas Degand, Chloé Plouzeau, Thomas Guillard, Vincent Cattoir, Asaf Mizrahi, Antoine Grillon, Frédéric Janvier, Cécile Le Brun, Marlène Amara, Mathilda Bastide, Alban Lemonnier, and Laurent Dortet
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Ceftolozane-tazobactam ,ESBL ,Carbapenemase ,Enterobacterales ,Epidemiology ,France ,Microbiology ,QR1-502 - Abstract
ABSTRACT: Objectives: Ceftolozane-tazobactam (C/T) proved its efficacy for the treatment of infections caused by non-carbapenemase producing Pseudomonas aeruginosa and Enterobacterales. Here, we aimed to provide susceptibility data on a large series of Enterobacterales since the revision of EUCAST categorization breakpoints in 2020. Methods: First, C/T susceptibility was determined on characterized Enterobacterales resistant to third generation cephalosporins (3GCs) (extended spectrum β-lactamase [ESBL] production or different levels of AmpC overexpression) (n = 213) and carbapenem-resistant Enterobacterales (CRE) (n = 259), including 170 carbapenemase producers (CPE). Then, 1632 consecutive clinical Enterobacterales responsible for infection were prospectively collected in 23 French hospitals. C/T susceptibility was determined by E-test® (biomérieux) and broth microdilution (BMD) (Sensititre™, Thermo Scientific) to perform method comparison. Results: Within the collection isolates, 88% of 3GC resistant strains were susceptible to C/T, with important variation depending on the resistance mechanism: 93% vs. 13% susceptibility for CTX-M and SHV-ESBL producers, respectively. Only 20% of the CRE were susceptible to C/T. Among CPE, 80% of OXA-48-like producers were susceptible to C/T, whereas all metallo-β-lactamase producers were resistant. The prospective study revealed that 95.6% of clinical isolates were susceptible to C/T. Method comparison performed on these 1632 clinical isolates demonstrated 99% of categorization agreement between MIC to C/T determined by E-test® in comparison with the BMD (reference) and only 74% of essential agreement. Conclusion: Overall, C/T showed good activity against wild-type Enterobacterales, AmpC producers, and ESBL-producing Escherichia coli but is less active against ESBL-producing Klebsiella pneumoniae, and CRE. E-test® led to an underestimation of the MICs in comparison to the BMD reference.
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- 2023
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40. YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitro
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Robin Caire, Estelle Audoux, Mireille Thomas, Elisa Dalix, Aurélien Peyron, Killian Rodriguez, Nicola Pordone, Johann Guillemot, Yann Dickerscheit, Hubert Marotte, François Vandenesch, Frédéric Laurent, Jérôme Josse, and Paul O. Verhoeven
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Science - Abstract
Growing evidence indicates that YAP/TAZ transcriptional regulators promote autophagy. Here, the authors characterize the role of YAP against Staphyloccocus aureus infection of synovial organoids and describe the role staphylococcal toxins have in antagonizing YAP-mediated functions.
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- 2022
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41. A fusidic acid-resistant (PVL+) clone is associated with the increase in methicillin-resistant Staphylococcus aureus in New Caledonia
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Alexandre Bourles, Anne Tristan, François Vandenesch, Michèle Bes, Frédéric Laurent, Anne-Gaëlle Ranc, Malia Kainiu, Ann-Claire Gourinat, Antoine Biron, Cécile Cazarola, Cyrille Goarant, and Julien Colot
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methicillin-resistant ,Staphylococcus aureus ,fusidic acid resistance ,New Caledonia ,Panton Valentin leukocidin ,community-acquired MRSA ,Microbiology ,QR1-502 - Abstract
Objectives: Since 2014, Staphylococcus aureus methicillin resistance has been rapidly increasing in New Caledonia and is associated with potential serious clinical repercussions. In the present study, we investigated the epidemiology of methicillin-resistant S. aureus (MRSA) in New Caledonia and the possible emergence of a particular clonal strain. Methods: An overview of the distribution of MRSA in New Caledonia in 2019 is presented. We collected and analysed 171 clinical MRSA isolates from New Caledonia medical laboratories during August and September 2019. Among this collection, 49 representative isolates were analyzed by the French National Reference Center for Staphylococci using the StaphyType DNA microarray, allowing genetic characterization of the isolates. Results: Among the 1144 S. aureus isolated over the year 2019, 442 isolates (39%) were resistant to methicillin, and 62% of these isolates were resistant to fusidic acid (FA). During the inclusion period, FA resistance rate was similar (60%). Genetic characterization evidenced CC6 as the predominant clonal complex (70%) with 26 isolates (53%) identified as CC6-MRSA-[IV+fus] (PVL+). Conclusions: These findings demonstrated a low diversity of MRSA in New Caledonia, with the dominance of a clonal complex not reported previously. The frequent fusidic acid (FA) resistance in MRSA was associated with a high prevalence of fusC gene, suggesting that FA misuse contributed to driving the selection of this clone. Our findings suggest the recommendation to stop the topical use of FA to control the emergence of this severe MRSA clone and decrease the rate of MRSA in New Caledonia.
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- 2022
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42. Clinical, Bacteriological, and Genetic Characterization of Bone and Joint Infections Involving Linezolid-Resistant Staphylococcus epidermidis: a Retrospective Multicenter Study in French Reference Centers
- Author
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François Coustillères, Victor Renault, Stéphane Corvec, Céline Dupieux, Patricia Martins Simões, Marie Frédérique Lartigue, Chloé Plouzeau-Jayle, Didier Tande, Claudie Lamoureux, Carole Lemarié, Rachel Chenouard, Frédéric Laurent, Adrien Lemaignen, and Pascale Bémer
- Subjects
linezolid-resistant multidrug-resistant Staphylococcus epidermidis ,bone and joint infections ,linezolid exposure ,sequence type ST2 ,multiresistance ,resistance to linezolide ,Microbiology ,QR1-502 - Abstract
ABSTRACT The choice of the best probabilistic postoperative antibiotics in bone and joint infections (BJIs) is still challenging. Since the implementation of protocolized postoperative linezolid in six French referral centers, linezolid-resistant multidrug-resistant Staphylococcus epidermidis (LR-MDRSE) strains were isolated in patients with BJI. We aimed here to describe clinical, microbiological, and molecular patterns associated with these strains. All patients with at least one intraoperative specimen positive for LR-MDRSE between 2015 and 2020 were included in this retrospective multicenter study. Clinical presentation, management, and outcome were described. LR-MDRSE strains were investigated by MIC determination for linezolid and other anti-MRSA antibiotics, characterization of genetic determinants of resistance, and phylogenetic analysis. Forty-six patients (colonization n = 10, infection n = 36) were included in five centers, 45 had prior exposure to linezolid, 33 had foreign devices. Clinical success was achieved for 26/36 patients. Incidence of LR-MDRSE increased over the study period. One hundred percent of the strains were resistant to oxazolidinones, gentamicin, clindamycin, ofloxacin, rifampicin, ceftaroline, and ceftobiprole, and susceptible to cyclins, daptomycin, and dalbavancin. Susceptibility to delafloxacin was bimodal. Molecular analysis was performed for 44 strains, and the main mutation conferring linezolid resistance was the 23S rRNA G2576T mutation. All strains belonged to the sequence type ST2 or its clonal complex, and phylogenetic analysis showed emergence of five populations corresponding geographically to the centers. We showed the emergence of new clonal populations of highly linezolid-resistant S. epidermidis in BJIs. Identifying patients at risk for LR-MDRSE acquisition and proposing alternatives to systematic postoperative linezolid use are essential. IMPORTANCE The manuscript describes the emergence of clonal linezolid-resistant strains of Staphylococcus epidermidis (LR-MDRSE) isolated from patients presenting with bone and joint infections. Incidence of LR-MDRSE increased over the study period. All strains were highly resistant to oxazolidinones, gentamicin, clindamycin, ofloxacin, rifampicin, ceftaroline, and ceftobiprole, but were susceptible to cyclins, daptomycin, and dalbavancin. Susceptibility to delafloxacin was bimodal. The main mutation conferring linezolid resistance was the 23S rRNA G2576T mutation. All strains belonged to the sequence type ST2 or its clonal complex, and phylogenetic analysis showed emergence of five populations corresponding geographically to the centers. LR-MDRSE bone and joint infections seem to be accompanied by an overall poor prognosis related to comorbidities and therapeutic issues. Identifying patients at risk for LR-MDRSE acquisition and proposing alternatives to systematic postoperative linezolid use become essential, with a preference for parenteral drugs such as lipopeptids or lipoglycopeptids.
- Published
- 2023
- Full Text
- View/download PDF
43. Epidemiology of infectious encephalitis, differences between a prospective study and hospital discharge data
- Author
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Benoit Guery, Jean-Louis Herrmann, Alexandra Mailles, Bruno Lina, Jean Paul Stahl, Daniel Terral, Marc Lecuit, Frederic Laurent, Cecile Bebear, Bruno POZZETTO, and Virginie Prendki
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,Epidemiology ,Mycobacterium tuberculosis ,Young Adult ,Internal medicine ,Infectious encephalitis ,Humans ,Medicine ,Prospective cohort study ,Aged ,biology ,business.industry ,Incidence (epidemiology) ,Bacterial Infections ,Middle Aged ,biology.organism_classification ,medicine.disease ,Original Papers ,Hospitals ,Infectious Diseases ,Virus Diseases ,Immunology ,Listeria ,Etiology ,Encephalitis ,Female ,France ,business - Abstract
SUMMARYThe French epidemiology of infectious encephalitis has been described in a 2007 prospective study. We compared these results with available data (demographic features, causative agents, case-fatality ratio) obtained through the French national hospital discharge 2007 database (PMSI), in order to evaluate it as a surveillance tool for encephalitis. Causative agents were identified in 52% of cases in the study, and 38% in PMSI (P Listeria monocytogenesandMycobacterium tuberculosiswere less frequent in PMSI than in the study (Listeria: 2%vs.5%,P = 0·001;Mycobacterium: 2%vs.8%,P Listeria(46% in the studyvs.16%). Nevertheless, despite the absence of case definitions and a possible misclassification weakening PMSI data, we suggest that PMSI may be used as a basic surveillance tool at a limited cost.
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- 2012
44. Pristinamycin in the treatment of MSSA bone and joint infection-authors' response
- Author
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Tristan Ferry, Francois Vandenesch, Jean-Philippe Rasigade, Frederic Laurent, Florent Valour, Florence ADER, Marc Janier, Pathogénie des Staphylocoques – Staphylococcal Pathogenesis (StaPath), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre interrégional de référence Rhône-Alpes - Auvergne des infections ostéo-articulaires complexes, Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Service de Maladies Infectieuses et Tropicales [Hôpital de la Croix-Rousse - HCL], Centre National de Reference des Staphylocoques, Université de Lyon, Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,030106 microbiology ,Arthritis ,Staphylococcal infections ,03 medical and health sciences ,chemistry.chemical_compound ,Internal medicine ,medicine ,Humans ,Pharmacology (medical) ,ComputingMilieux_MISCELLANEOUS ,Pristinamycin ,Pharmacology ,Arthritis, Infectious ,business.industry ,Infectious ,Staphylococcal Infections ,medicine.disease ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Anti-Bacterial Agents ,Infectious Diseases ,chemistry ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,business - Abstract
International audience
- Published
- 2016
45. Emergence and dissemination of a linezolid-resistant Staphylococcus capitis clone in France
- Author
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Frederic Laurent
- Published
- 2016
46. Reliability of the Xpert MRSA NxG assay and the BD MAX MRSA XT assay to detect genetically diverse mecA/mecC MRSA and mecA drop-out MSSA isolates from Europe
- Author
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Frederic Laurent
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- 2016
47. Whole-Genome Sequencing for Routine Pathogen Surveillance in Public Health: a Population Snapshot of Invasive Staphylococcus aureus in Europe
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David M. Aanensen, Edward J. Feil, Matthew T. G. Holden, Janina Dordel, Corin A. Yeats, Artemij Fedosejev, Richard Goater, Santiago Castillo-Ramírez, Jukka Corander, Caroline Colijn, Monika A. Chlebowicz, Leo Schouls, Max Heck, Gerlinde Pluister, Raymond Ruimy, Gunnar Kahlmeter, Jenny íÅhman, Erika Matuschek, Alexander W. Friedrich, Julian Parkhill, Stephen D. Bentley, Brian G. Spratt, Hajo Grundmann, Helmut Mittermayer, Karina Krziwanek, Sabine Stumvoll, Walter Koller, Olivier Denis, Marc Struelens, Dimitr Nashev, Ana Budimir, Smilja Kalenic, Despo Pieridou-Bagatzouni, Vladislav Jakubu, Helena Zemlickova, Henrik Westh, Anders Rhod Larsen, Robert Skov, Frederic Laurent, Jerome Ettienne, Birgit Strommenger, Wolfgang Witte, Sofia Vourli, Alkis Vatopoulos, Anni Vainio, Jaana Vuopio-Varkila, Miklos Fuzi, Erika UngvíÅóóóári, Stephan Murchan, Angela Rossney, Edvins Miklasevics, Arta Balode, Gunnsteinn Haraldsson, Karl G. Kristinsson, Monica Monaco, Annalisa Pantosti, Michael Borg, Marga van Santen-Verheuvel, Xander Huijsdens, Lillian Marstein, Trond Jacobsen, Gunnar Skov Simonsen, Marta Airesde-Sousa, Herminia de Lencastre, Agnieszka Luczak-Kadlubowska, Waleria Hryniewicz, Monica Straut, Irina Codita, Maria Perez-Vazquez, Jesus Oteo Iglesias, Vesna Cvitkovic Spik, Manica Mueller-Premru, Sara Haeggman, Barbro Olsson-Liljequist, Matthew Ellington, Angela Kearns, Wellcome Trust, Medical Research Council (Reino Unido), University of St Andrews. School of Medicine, University of St Andrews. Infection Group, University of St Andrews. Biomedical Sciences Research Complex, Microbes in Health and Disease (MHD), National Institute for Health Research, and Medical Research Council (MRC)
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0301 basic medicine ,METHICILLIN-RESISTANT ,Population level ,MRSA ,Disease Outbreaks ,RA0421 ,RA0421 Public health. Hygiene. Preventive Medicine ,education.field_of_study ,SINGLE-NUCLEOTIDE POLYMORPHISMS ,High-Throughput Nucleotide Sequencing ,Staphylococcal Infections ,QR1-502 ,3. Good health ,COMMUNITY ,Europe ,Epidemiological Monitoring ,Snapshot (computer storage) ,epidemiology ,BDC ,Research Article ,STRAIN ,Staphylococcus aureus ,medicine.medical_specialty ,GENES ,030106 microbiology ,Population ,NDAS ,Context (language use) ,Biology ,Microbiology ,03 medical and health sciences ,Antibiotic resistance ,SDG 3 - Good Health and Well-being ,Virology ,CLONE ,Drug Resistance, Bacterial ,medicine ,Humans ,Computer Simulation ,Road map ,education ,Whole genome sequencing ,business.industry ,Public health ,microbiology ,Computational Biology ,Sequence Analysis, DNA ,Data science ,EVOLUTION ,Ingénierie biomédicale ,Biotechnology ,VIRULENCE ,The spread of antibiotic-resistant bacteria is a public health emergency of global concern, threatening medical intervention at every level of health care delivery ,Microbiologie et protistologie [bacteriol.virolog.mycolog.] ,business ,Genome, Bacterial ,Software ,European SRL Working Group ,TOXIC-SHOCK-SYNDROME - Abstract
The implementation of routine whole-genome sequencing (WGS) promises to transform our ability to monitor the emergence and spread of bacterial pathogens. Here we combined WGS data from 308 invasive Staphylococcus aureus isolates corresponding to a pan-European population snapshot, with epidemiological and resistance data. Geospatial visualization of the data is made possible by a generic software tool designed for public health purposes that is available at the project URL (http:// www.microreact.org/project/EkUvg9uY?tt=rc). Our analysis demonstrates that high-risk clones can be identified on the basis of population level properties such as clonal relatedness, abundance, and spatial structuring and by inferring virulence and resistance properties on the basis of gene content. We also show that in silico predictions of antibiotic resistance profiles are at least as reliable as phenotypic testing. We argue that this work provides a comprehensive road map illustrating the three vital components for future molecular epidemiological surveillance: (i) large-scale structured surveys, (ii) WGS, and (iii) communityoriented database infrastructure and analysis tools. IMPORTANCE The spread of antibiotic-resistant bacteria is a public health emergency of global concern, threatening medical intervention at every level of health care delivery. Several recent studies have demonstrated the promise of routine wholegenome sequencing (WGS) of bacterial pathogens for epidemiological surveillance, outbreak detection, and infection control. However, as this technology becomes more widely adopted, the key challenges of generating representative national and international data sets and the development of bioinformatic tools to manage and interpret the data become increasingly pertinent. This study provides a road map for the integration of WGS data into routine pathogen surveillance. We emphasize the importance of large-scale routine surveys to provide the population context for more targeted or localized investigation and the development of open-access bioinformatic tools to provide the means to combine and compare independently generated data with publicly available data sets., 0, SCOPUS: ar.j, info:eu-repo/semantics/published
- Published
- 2016
48. Disappearance of FDG uptake on PET scan after antimicrobial therapy could help for the diagnosis of Coxiella burnetii spondylodiscitis
- Author
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Tristan Ferry, Francois Vandenesch, Jean-Philippe Rasigade, Frederic Laurent, Florent Valour, Florence ADER, Marc Janier, Service de Maladies Infectieuses et Tropicales [Hôpital de la Croix-Rousse - HCL], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Laboratoire de Bactériologie de l'Hôpital de la Croix-Rousse, Hospices Civils de Lyon (HCL), Pathogénie des Staphylocoques – Staphylococcal Pathogenesis (StaPath), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,Spondylodiscitis ,Male ,medicine.medical_specialty ,Discitis ,Biopsy ,030106 microbiology ,Polymerase Chain Reaction ,Article ,03 medical and health sciences ,Anti-Infective Agents ,Fluorodeoxyglucose F18 ,SANTE ,medicine ,Back pain ,Humans ,2. Zero hunger ,medicine.diagnostic_test ,business.industry ,Soft tissue ,Magnetic resonance imaging ,General Medicine ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,3. Good health ,Surgery ,Positron emission tomography ,Back Pain ,Coxiella burnetii ,Doxycycline ,Positron-Emission Tomography ,Chills ,medicine.symptom ,business ,Nuclear medicine ,Q Fever ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Hydroxychloroquine - Abstract
A 55 year-old man was admitted for worsening of a chronic low back pain associated with L4-L5 anterolisthesis, despite taking non-steroidal anti-inflammatory drugs for several months. He had a medical history of high blood pressure and obesity (body mass index, 37 kg/m2). He lived in the countryside but had no direct contact with animals except his dog. There were no fever, chills, sweats or weight loss. C reactive protein (CRP) was
- Published
- 2016
49. Teicoplanin-based antimicrobial therapy in Staphylococcus aureus bone and joint infection (BJI): tolerance, efficacy and experience with subcutaneous (SC) administration
- Author
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Tristan Ferry, Olivier Peeters, André Boibieux, Evelyne Braun, Anissa Bouaziz, Judith Karsenty, Frederic Laurent, Sébastien Lustig, Emmanuel Forestier, Florence Ader, Christian Chidiac, Florent Valour, and Lyon BJI study group
- Subjects
medicine.medical_specialty ,Teicoplanin ,business.industry ,Antimicrobial ,Joint infections ,medicine.disease_cause ,Infectious Diseases ,Oncology ,Staphylococcus aureus ,Internal medicine ,Immunology ,medicine ,business ,medicine.drug - Published
- 2016
50. Worldwide Endemicity of a Multidrug-Resistant Staphylococcus capitis Clone Involved in Neonatal Sepsis
- Author
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Marine Butin, Jean-Philippe Rasigade, Team1 Carmen, Frederic Laurent, Patricia M Simoes, and Carmen Lab
- Subjects
0301 basic medicine ,Microbiology (medical) ,neonatal sepsis ,Endemic Diseases ,Epidemiology ,multidrug-resistant ,030106 microbiology ,Worldwide Endemicity of a Multidrug-Resistant Staphylococcus capitis Clone Involved in Neonatal Sepsis ,Clone (cell biology) ,lcsh:Medicine ,Biology ,worldwide ,Global Health ,Staphylococcal infections ,epidemiologic relevance ,lcsh:Infectious and parasitic diseases ,clone NRCS-A ,Microbiology ,Staphylococcus capitis ,03 medical and health sciences ,0302 clinical medicine ,Antibiotic resistance ,Drug Resistance, Multiple, Bacterial ,Intensive care ,Research Letter ,medicine ,Global health ,Humans ,lcsh:RC109-216 ,antimicrobial resistance ,030212 general & internal medicine ,bacteria ,Staphylococci ,Neonatal sepsis ,lcsh:R ,Infant, Newborn ,Staphylococcal Infections ,medicine.disease ,biology.organism_classification ,Anti-Bacterial Agents ,Multiple drug resistance ,Infectious Diseases ,endemic ,neonatal intensive care units - Abstract
A multidrug-resistant Staphylococcus capitis clone, NRCS-A, has been isolated from neonatal intensive care units in 17 countries throughout the world. S. capitis NRCS-A prevalence is high in some neonatal intensive care units in France. These data highlight the worldwide endemicity and epidemiologic relevance of this multidrug-resistant, coagulase-negative staphylococci clone.
- Published
- 2017
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