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The intense pressure on the poultry sector to promote safe chicken meat and egg has sparked interest in the use of natural and consumer-accepted phytogenic feed additives. The majority of literature on the benefits of turmeric (Curcuma longa) and Negro pepper (Xylopia aethiopica) is focused on layers and there is no evidence of turmeric and Negro pepper interactive effects. Therefore, this study was conducted to evaluate the effect of turmeric and Negro pepper mixture on serum biochemical and haematological constituents of broiler chicks fed for 48 days. A total of 96 one-day-old Arbor acres chicks of mixed sexes were used for the experiment. The birds were randomly assigned to four treatment groups and replicated three times in a completely randomized design. The milled turmeric and Negro pepper were mixed in the ratio of 50:50 (50%) and used in the formulation of turmeric and Negro pepper mixture (TNPM) diets. The four experimental diets were formulated to contain 0.00, 0.50, 1.00, and 1.50% dietary levels of inclusion of TNPM at the starter phase and 0.00, 1.50, 3.00, and 4.50% inclusion levels at the finishing phase respectively. Blood samples were collected from the brachial wing vein on the 24th and 48th days of the experiment and were evaluated for serum biochemical and haematological constituents. At the starter phase, red blood cells (RBC) was significantly (P