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1. A carbonate system time series in the Eastern Mediterranean Sea. Two years of high-frequency in-situ observations and remote sensing

Author

Frangoulis, C., primary, Stamataki, N., additional, Pettas, M., additional, Michelinakis, S., additional, King, A. L., additional, Giannoudi, L., additional, Tsiaras, K., additional, Christodoulaki, S., additional, Seppälä, J., additional, Thyssen, M., additional, Borges, A.V., additional, and Krasakopoulou, E., additional

Published
2024
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4. The Hellenic Marine Observing, Forecasting and Technology System—An Integrated Infrastructure for Marine Research

Author

Bourma, E. Perivoliotis, L. Petihakis, G. Korres, G. Frangoulis, C. Ballas, D. Zervakis, V. Tragou, E. Katsafados, P. Spyrou, C. Dassenakis, M. Poulos, S. Megalofonou, P. Sofianos, S. Paramana, T. Katsaounis, G. Karditsa, A. Petrakis, S. Mavropoulou, A.-M. Paraskevopoulou, V. Milatou, N. Pagonis, P. Velanas, S. Ntoumas, M. Mamoutos, I. Pettas, M. Christodoulaki, S. Kassis, D. Sotiropoulou, M. Mavroudi, A. Moira, A. Denaxa, D. Anastasopoulou, G. Potiris, E. Kolovogiannis, V. Dimitrakopoulos, A.-A. Petalas, S. Zissis, N.

Abstract

Research infrastructures have been established throughout Europe in order to create robust organizations that will facilitate and enhance research and innovation processes and will advance society with innovative products and services. The Hellenic Integrated Marine Observing, orecasting and Technology System (component of HIMIOFoTS RI) has been implemented in the framework of the National Roadmap for Research Infrastructures to form a large-scale infrastructure for the marine environment in Greece. It links together ocean observing and forecasting systems, coastal zone monitoring and management practices, as well as ocean engineering testing facilities. The overarching framework of the system supports the coordination of five organizations with expertise in the field of marine science and technology, the central management of research activities, and the common development of services and products. It comprises facilities and resources while it provides open access to research communities (academia, industry) to support the scientific advancements and innovation in their fields. The Hellenic Marine Observing, Forecasting and Technology System was further enhanced during its implementation through significant upgrades and developments in order to extend its observing capacity and the forecasting and technological abilities, while advancing the provided services and products. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Published
2022

6. Seasonal variations of biochemical and optical properties, physical dynamics and N stable isotopic composition in three northeastern Mediterranean basins (Aegean, Cretan and Ionian Seas)

Author

Pavlidou, A., primary, Velaoras, D., additional, Karageorgis, A.P., additional, Rousselaki, E., additional, Parinos, C., additional, Dähnke, K., additional, Möbius, J., additional, Meador, T.B., additional, Psarra, S., additional, Frangoulis, C., additional, Souvermezoglou, E., additional, Androni, A., additional, Assimakopoulou, G., additional, Chaikalis, S., additional, Kanellopoulos, T.D., additional, Lagaria, A., additional, Zachioti, P., additional, and Gogou, A., additional

Published
2020
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8. Challenges for Sustained Observing and Forecasting Systems in the Mediterranean Sea

Author

Tintoré, J. Pinardi, N. Álvarez-Fanjul, E. Aguiar, E. Álvarez-Berastegui, D. Bajo, M. Balbin, R. Bozzano, R. Nardelli, B.B. Cardin, V. Casas, B. Charcos-Llorens, M. Chiggiato, J. Clementi, E. Coppini, G. Coppola, L. Cossarini, G. Deidun, A. Deudero, S. D'Ortenzio, F. Drago, A. Drudi, M. El Serafy, G. Escudier, R. Farcy, P. Federico, I. Fernández, J.G. Ferrarin, C. Fossi, C. Frangoulis, C. Galgani, F. Gana, S. García Lafuente, J. Sotillo, M.G. Garreau, P. Gertman, I. Gómez-Pujol, L. Grandi, A. Hayes, D. Hernández-Lasheras, J. Herut, B. Heslop, E. Hilmi, K. Juza, M. Kallos, G. Korres, G. Lecci, R. Lazzari, P. Lorente, P. Liubartseva, S. Louanchi, F. Malacic, V. Mannarini, G. March, D. Marullo, S. Mauri, E. Meszaros, L. Mourre, B. Mortier, L. Muñoz-Mas, C. Novellino, A. Obaton, D. Orfila, A. Pascual, A. Pensieri, S. Pérez Gómez, B. Pérez Rubio, S. Perivoliotis, L. Petihakis, G. de la Villéon, L.P. Pistoia, J. Poulain, P.-M. Pouliquen, S. Prieto, L. Raimbault, P. Reglero, P. Reyes, E. Rotllan, P. Ruiz, S. Ruiz, J. Ruiz, I. Ruiz-Orejón, L.F. Salihoglu, B. Salon, S. Sammartino, S. Sánchez Arcilla, A. Sánchez-Román, A. Sannino, G. Santoleri, R. Sardá, R. Schroeder, K. Simoncelli, S. Sofianos, S. Sylaios, G. Tanhua, T. Teruzzi, A. Testor, P. Tezcan, D. Torner, M. Trotta, F. Umgiesser, G. von Schuckmann, K. Verri, G. Vilibic, I. Yucel, M. Zavatarelli, M. Zodiatis, G.

Abstract

The Mediterranean community represented in this paper is the result of more than 30 years of EU and nationally funded coordination, which has led to key contributions in science concepts and operational initiatives. Together with the establishment of operational services, the community has coordinated with universities, research centers, research infrastructures and private companies to implement advanced multi-platform and integrated observing and forecasting systems that facilitate the advancement of operational services, scientific achievements and mission-oriented innovation. Thus, the community can respond to societal challenges and stakeholders needs, developing a variety of fit-for-purpose services such as the Copernicus Marine Service. The combination of state-of-the-art observations and forecasting provides new opportunities for downstream services in response to the needs of the heavily populated Mediterranean coastal areas and to climate change. The challenge over the next decade is to sustain ocean observations within the research community, to monitor the variability at small scales, e.g., the mesoscale/submesoscale, to resolve the sub-basin/seasonal and inter-annual variability in the circulation, and thus establish the decadal variability, understand and correct the model-associated biases and to enhance model-data integration and ensemble forecasting for uncertainty estimation. Better knowledge and understanding of the level of Mediterranean variability will enable a subsequent evaluation of the impacts and mitigation of the effect of human activities and climate change on the biodiversity and the ecosystem, which will support environmental assessments and decisions. Further challenges include extending the science-based added-value products into societal relevant downstream services and engaging with communities to build initiatives that will contribute to the 2030 Agenda and more specifically to SDG14 and the UN's Decade of Ocean Science for sustainable development, by this contributing to bridge the science-policy gap. The Mediterranean observing and forecasting capacity was built on the basis of community best practices in monitoring and modeling, and can serve as a basis for the development of an integrated global ocean observing system. © Copyright © 2019 Tintoré, Pinardi.

Published
2019

10. Phytoplankton variability and community structure in relation to hydrographic features in the NE Aegean frontal area (NE Mediterranean Sea)

Author

Lagaria, A. Mandalakis, M. Mara, P. Frangoulis, C. Karatsolis, B.-T. Pitta, P. Triantaphyllou, M. Tsiola, A. Psarra, S.

Subjects
fungi
Abstract

The structure of phytoplankton community in the salinity-stratified Northeastern Aegean frontal area adjacent to the Dardanelles Straits was investigated on a seasonal basis (autumn, spring and summer) and in relation to circulating water masses: the modified Black Sea Water (BSW) and the Levantine Water (LW). By employing High Performance Liquid Chromatography (HPLC) for the analysis of phytoplankton pigments in conjunction with conventional cell counting methodologies (i.e. inverted light microscopy, flow cytometry) and primary production measurements, a comprehensive qualitative and quantitative characterization of phytoplankton community composition and its activity was conducted. Chlorophyll-a normalized production and estimated growth rates presented the highest values within the ‘fresh’ BSW mass during summer, though generally growth rates were low (

Published
2017

11. Trying to resolve the taxonomic confusion of Paracalanus parvus species complex (Copepoda, Calanoida) in the Mediterranean and Black Seas through a combined analysis of morphology, molecular taxonomy and DNA metabarcoding

Author

Kasapidis, P. (Panagiotis), Siokou-Frangou, I. (Ioanna), Khelifi-Touhami, M. (Meriem), Mazzocchi, M.G. (Maria Grazia), Christou, E.D. (Epaminondas D.), Fernández-de-Puelles, M.L. (María Luz), Gubanova, A. (Alexandra), Di-Capua, I. (Iole), Santandreu, M.M. (María Mar), Terzoglou, V. (Vassiliki), Batziakas, E. (Efstratios), and Frangoulis, C. (Constantin)

Subjects
next generation sequencing, taxonomy, cyrochrome oxidase I, phylogenetic analysis, Paracalanus parvus species complex
Abstract

Paracalanus parvus is reported as the most abundant representative of the genus and one of the main components of the coastal zooplankton in the Mediterranean and Black Seas. However, the subtle taxonomic differences between P. parvus and the congeneric species P. indicus and P. quasimodo, in combination with the ample morphological variation found in Mediterranean specimens, render problematic the correct identification. A recent molecular study by Cornils and Held (2014) provided evidence of cryptic speciation in the P. parvus complex and indicated that P. parvus s.s. does not have global distribution, but may be restricted to the northeastern Atlantic. In order to clarify the taxonomic status and distribution of this species complex in the Mediterranean and Black Seas, a study was conducted on Paracalanus specimens collected from different locations across the aforementioned marine basins and sequenced for portion of the COI mitochondrial gene. An accurate taxonomic analysis was also carried out to correlate morphological characteristics with the molecular species' assignation. The phylogenetic analysis of the specimens together with the publicly available sequences of P. parvus complex revealed the presence of four molecular operational taxonomic units (MOTUs) in the Mediterranean, which differed in abundance and geographic distribution. The combination of morphological and molecular data revealed great inconsistencies between morphospecies and MOTUs. Moreover, several bulk zooplankton samples were analyzed through DNA metabarcoding in the frame of the “MetaCopepod” project to provide more extensive information on the spatiotemporal distribution and abundance of the target species

Published
2016

12. The 'MetaCopepod' project: Designing an integrated DNA metabarcoding and image analysis approach to study and monitor the diversity of zooplanktonic copepods and cladocerans in the Mediterranean Sea

Author

Kasapidis, P. (Panagiotis), Frangoulis, C. (Constantin), Siokou-Frangou, I. (Ioanna), Batziakas, E. (Efstratios), Lagnel, J. (Jacques), Manousaki, T. (Tereza), Christou, E.D. (Epaminondas D.), Mazzocchi, M.G. (Maria Grazia), Khelifi-Touhami, M. (Meriem), Fernández-de-Puelles, M.L. (María Luz), Ak-Örek, Y. (Yesim), Gubanova, A. (Alexandra), Abu-Alhaija, R. (Rana), Faulweter, S. (Sarah), Tsartsianidou, V. (Valentina), and Zampicinini, G. (Gianpaolo)

Subjects
zooplankton, Copepoda, image analysis, Mediterranean Sea, Cladocera, DNA metabarcoding
Abstract

The timely and accurate analysis of marine zooplankton diversity is a challenge in ecological and monitoring studies. Morphology-based identification of taxa, which requires taxonomy experts, is time consuming and cannot provide accurate resolution at species level in several cases (e.g. immature stages, cryptic species, broken specimens). The “MetaCopepod” project is aimed at overcoming these limitations by developing a high-throughput and cost effective methodology that integrates DNA metabarcoding and image analysis. Utilizing the accuracy of DNA metabarcoding in species recognition and the quantitative results of image analysis, zooplankton diversity (mainly of copepods and cladocerans) is assessed both qualitatively (species' composition) and quantitatively (abundance, biomass and size-distribution). To achieve this goal, bulk zooplankton samples are first scanned and analyzed with ZooImage and then massively sequenced for a selected fragment of the mitochondrial 16S rRNA gene. Through a bioinformatic pipeline, sequences are compared to a reference genetic database, constructed within the project, and identified at species- level. The methodology was calibrated by using both mock and taxonomically identified samples and demonstrated on samples collected monthly from monitoring stations across the Mediterranean Sea. It is currently optimized for higher integration and accuracy and is expected to become a powerful tool for monitoring zooplankton in the long term and for early warning of bioinvasions and other ecosystem changes

Published
2016

17. Comparison of marine copepod outfluxes: Nature, rate, fate and role in the carbon and nitrogen cycles

Author

Frangoulis, C., Christou, E.D., and Hecq, J.H.

Subjects
Copepoda, Copepoda [copepods], digestive, oral, and skin physiology, fungi, Carbon cycle, Nitrogen cycle, human activities
Abstract

We compare the nature of copepod outfluxes of nonliving matter, the factors controlling their rate (aid their Jaw, and finally their role, particularly their relative importance in the carbon and nitrogen cycle. Copepods release dissolved matter through excretion and respiration and particulate matter through production of faecal pellets, carcasses, moults, and (lead eggs. Excretion liberates several organic C, N, and P compounds and inorganic N and P compounds, with inorganic compounds constituting the larger part. The faecal pellets of copepods are covered by a peritrophic membrane and have a highly variable size and content. There is less information on the nature of other copepod particulate products. The weight-specific rates of posthatch mortality, respiration, excretion, and faecal pellet production have similar C or N levels and are higher than those of moulting and egg mortality. In general, most important factors controlling these rates are temperature, body mass, food concentration, food quality, and faunistic composition. Physical and biological factors govern the vertical fate of copepod products by affecting their sedimentation speed and concentration gradient. The physical factors are sinking speed, advection, stratification, turbulent diffiusion, and molecular diffusion. They influence the sedimentation speed and degradation of the copepod products. The biological factors are production, biodegradation (by zooplankton, nekton, and microorganisms) and vertical migration of copepods (diel or seasonal). Physical degradation and biodegradation by zooplankton and nekton are faster than biodegradation by microorganisms. The most important copepod outfluxes are excretion and fecal pellet production. Excretion offers inorganic nutrients that can be directly used by . Excretion of primary producers. Faecal pellets have a more important role in the vertical transport of elements than the other particulate products. Most investigation has focused on carbon burial in the form of copepod faecal pellets, measured by sediment traps, and on the role of ammonia excretion in nutrient recycling. Full evaluation of the role of copepod products in the transport and recycling of elements and compounds requires a quantification of all copepod products and their different fates, particularly detritiphagy, remineralization, and integration as marine snow.

Published
2005

24. Adamantiades-Behcet syndrome. Current concepts

Author

Georgiades, G., Sinakos, Z., Frangoulis, C., Psilas, K., Economou, T., Karyofillis, K., and Kambouroglou, J.

Subjects
Adult, Male, Adolescent, Humans, Female, Behcet Syndrome/*diagnosis/pathology/therapy, Middle Aged
Abstract

Bull Mem Soc Fr Ophtalmol

Published
1976

25. Challenges for Sustained Observing and Forecasting Systems in the Mediterranean Sea

Author

Emma Heslop, Javier Ruiz, François Galgani, Pablo Lorente, Ferial Louanchi, Pierre Testor, Susana Pérez Rubio, Romain Escudier, Alejandro Orfila, Roberto Bozzano, Georg Umgiesser, George Petihakis, Marc Torner, Sara Pensieri, Patrick Raimbault, George Zodiatis, Mélanie Juza, Patrick Farcy, Baris Salihoglu, David March, Simona Simoncelli, Daniel J. Hayes, Simón Ruiz, Paolo Lazzari, Giorgia Verri, Francesco Trotta, Loic Petit De La Villeon, Emanuela Clementi, Benjamín Casas, Joaquín Tintoré, D. Obaton, Constantin Frangoulis, Marco Bajo, Miguel Charcos-Llorens, Gianandrea Mannarini, Antonio Sánchez-Román, Vlado Malačič, Patricia Reglero, Edgar G. Pavia, Elena Mauri, Juan Gabriel Fernández, Rosalia Santoleri, Paz Rotllan, Svitlana Liubartseva, Toste Tanhua, Sylvie Pouliquen, Laurent Coppola, Gianpiero Cossarini, Leonidas Perivoliotis, Bruno Buongiorno Nardelli, Cristian Munoz-Mas, Diego Álvarez-Berastegui, Laurent Mortier, Antonio Novellino, Isaac Gertman, Luis F. Ruiz-Orejón, Slim Gana, Jacopo Chiggiato, Gerasimos Korres, Aldo Drago, Pierre-Marie Poulain, Inmaculada Ruiz, Eva Aguiar, Marcos García Sotillo, Katrin Schroeder, Gianmaria Sannino, Anna Teruzzi, Jesús García Lafuente, Baptiste Mourre, Ivica Vilibić, Karina von Schuckmann, Lorinc Meszaros, Emma Reyes, Marco Zavatarelli, Lluís Gómez-Pujol, Simone Sammartino, George Kallos, Jaime Hernandez-Lasheras, Vanessa Cardin, Enrique Álvarez-Fanjul, Rosa Balbin, Karim Hilmi, Massimiliano Drudi, Devrim Tezcan, Alan Deidun, Pierre Garreau, Sarantis Sofianos, Alessandro Grandi, Barak Herut, Cristina Fossi, Stefano Salon, Ghada El Serafy, J. Pistoia, Begoña Pérez Gómez, Giovanni Coppini, Salvatore Marullo, Rafael Sardá, Mustafa Yücel, Fabrizio D'Ortenzio, Laura Prieto, Salud Deudero, Agustín Sánchez Arcilla, Rita Lecci, Georgios Sylaios, Ivan Federico, Ananda Pascual, Christian Ferrarin, Nadia Pinardi, Tintore, J., Pinardi, N., Alvarez-Fanjul, E., Aguiar, E., Alvarez-Berastegui, D., Bajo, M., Balbin, R., Bozzano, R., Nardelli, B. B., Cardin, V., Casas, B., Charcos-Llorens, M., Chiggiato, J., Clementi, E., Coppini, G., Coppola, L., Cossarini, G., Deidun, A., Deudero, S., D'Ortenzio, F., Drago, A., Drudi, M., El Serafy, G., Escudier, R., Farcy, P., Federico, I., Fernandez, J. G., Ferrarin, C., Fossi, C., Frangoulis, C., Galgani, F., Gana, S., Garcia Lafuente, J., Sotillo, M. G., Garreau, P., Gertman, I., Gomez-Pujol, L., Grandi, A., Hayes, D., Hernandez-Lasheras, J., Herut, B., Heslop, E., Hilmi, K., Juza, M., Kallos, G., Korres, G., Lecci, R., Lazzari, P., Lorente, P., Liubartseva, S., Louanchi, F., Malacic, V., Mannarini, G., March, D., Marullo, S., Mauri, E., Meszaros, L., Mourre, B., Mortier, L., Munoz-Mas, C., Novellino, A., Obaton, D., Orfila, A., Pascual, A., Pensieri, S., Perez Gomez, B., Perez Rubio, S., Perivoliotis, L., Petihakis, G., de la Villeon, L. P., Pistoia, J., Poulain, P. -M., Pouliquen, S., Prieto, L., Raimbault, P., Reglero, P., Reyes, E., Rotllan, P., Ruiz, S., Ruiz, J., Ruiz, I., Ruiz-Orejon, L. F., Salihoglu, B., Salon, S., Sammartino, S., Sanchez Arcilla, A., Sanchez-Roman, A., Sannino, G., Santoleri, R., Sarda, R., Schroeder, K., Simoncelli, S., Sofianos, S., Sylaios, G., Tanhua, T., Teruzzi, A., Testor, P., Tezcan, D., Torner, M., Trotta, F., Umgiesser, G., von Schuckmann, K., Verri, G., Vilibic, I., Yucel, M., Zavatarelli, M., Zodiatis, G., Institut Mediterrani d'Estudis Avancats (IMEDEA), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)-Universidad de las Islas Baleares (UIB), University of Bologna/Università di Bologna, SOCIB Balearic Islands Coastal Ocean Observing and Forecasting System, Istituto di Scienze Marine [Bologna] (ISMAR), Istituto di Science Marine (ISMAR ), National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR)-National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Cyprus Oceanography Center, University of Cyprus [Nicosia] (UCY), Variabilité de l'Océan et de la Glace de mer (VOG), Laboratoire d'Océanographie et du Climat : Expérimentations et Approches Numériques (LOCEAN), Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Centre Oceanogràfic de les Balears [Palma, Spain] (COB), Instituto Espagňol de Oceanografia (IEO), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Helmholtz Centre for Ocean Research [Kiel] (GEOMAR), University of Bologna, Consiglio Nazionale delle Ricerche (CNR)-Consiglio Nazionale delle Ricerche (CNR), Consiglio Nazionale delle Ricerche (CNR), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), University of Cyprus (UCY), Institut Pierre-Simon-Laplace (IPSL (FR_636)), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut de Recherche pour le Développement (IRD)-Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut de Recherche pour le Développement (IRD)-Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU), Universitat Politècnica de Catalunya. Departament d'Enginyeria Civil i Ambiental, Universitat Politècnica de Catalunya. LIM/UPC - Laboratori d'Enginyeria Marítima, Tintore J., Pinardi N., Alvarez-Fanjul E., Aguiar E., Alvarez-Berastegui D., Bajo M., Balbin R., Bozzano R., Nardelli B.B., Cardin V., Casas B., Charcos-Llorens M., Chiggiato J., Clementi E., Coppini G., Coppola L., Cossarini G., Deidun A., Deudero S., D'Ortenzio F., Drago A., Drudi M., El Serafy G., Escudier R., Farcy P., Federico I., Fernandez J.G., Ferrarin C., Fossi C., Frangoulis C., Galgani F., Gana S., Garcia Lafuente J., Sotillo M.G., Garreau P., Gertman I., Gomez-Pujol L., Grandi A., Hayes D., Hernandez-Lasheras J., Herut B., Heslop E., Hilmi K., Juza M., Kallos G., Korres G., Lecci R., Lazzari P., Lorente P., Liubartseva S., Louanchi F., Malacic V., Mannarini G., March D., Marullo S., Mauri E., Meszaros L., Mourre B., Mortier L., Munoz-Mas C., Novellino A., Obaton D., Orfila A., Pascual A., Pensieri S., Perez Gomez B., Perez Rubio S., Perivoliotis L., Petihakis G., de la Villeon L.P., Pistoia J., Poulain P.-M., Pouliquen S., Prieto L., Raimbault P., Reglero P., Reyes E., Rotllan P., Ruiz S., Ruiz J., Ruiz I., Ruiz-Orejon L.F., Salihoglu B., Salon S., Sammartino S., Sanchez Arcilla A., Sanchez-Roman A., Sannino G., Santoleri R., Sarda R., Schroeder K., Simoncelli S., Sofianos S., Sylaios G., Tanhua T., Teruzzi A., Testor P., Tezcan D., Torner M., Trotta F., Umgiesser G., von Schuckmann K., Verri G., Vilibic I., Yucel M., Zavatarelli M., and Zodiatis G.

Subjects
0106 biological sciences, Science with and for society, Service (systems architecture), lcsh:QH1-199.5, Nature observation, 010504 meteorology & atmospheric sciences, Climate, SDG's, Oceanography, 01 natural sciences, Operations research -- Case studies, sustained observations, 11. Sustainability, observing and forecasting system, Observing and forecasting systems, lcsh:Science, Water Science and Technology, Global and Planetary Change, Enginyeria agroalimentària::Ciències de la terra i de la vida::Climatologia i meteorologia [Àrees temàtiques de la UPC], SDG', observing and forecasting systems, ocean variability, FAIR data, climate, operational services, science with and for society, Prediction theory, Climatology -- Data processing, Environmental resource management, Seawater -- Mediterranean Region, operational service, Operational services, Variety (cybernetics), Mediterranean Sea--Climate, Ocean variability, Quantitative research -- Evaluation, Mediterrània, Mar -- Aspectes ambientals, Ocean observations, SDG’s, Best practice, Climate change, Ocean Engineering, lcsh:General. Including nature conservation, geographical distribution, Aquatic Science, Sustained observations, 12. Responsible consumption, 14. Life underwater, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography, 0105 earth and related environmental sciences, Downstream (petroleum industry), Sustainable development, Ensemble forecasting, business.industry, 010604 marine biology & hydrobiology, Enginyeria civil::Enginyeria hidràulica, marítima i sanitària::Ports i costes [Àrees temàtiques de la UPC], 13. Climate action, lcsh:Q, observing and forecasting systems, sustained observations, ocean variability, FAIR data, climate, operational services, science with and for society, SDG’s, Business
Abstract

Este artículo contiene 30 páginas, 14 figuras., The Mediterranean community represented in this paper is the result of more than 30 years of EU and nationally funded coordination, which has led to key contributions in science concepts and operational initiatives. Together with the establishment of operational services, the community has coordinated with universities, research centers, research infrastructures and private companies to implement advanced multi-platform and integrated observing and forecasting systems that facilitate the advancement of operational services, scientific achievements and mission-oriented innovation. Thus, the community can respond to societal challenges and stakeholders needs, developing a variety of fit-for-purpose services such as the Copernicus Marine Service. The combination of state-of-the-art observations and forecasting provides new opportunities for downstreamservices in response to the needs of the heavily populatedMediterranean coastal areas and to climate change. The challenge over the next decade is to sustain ocean observations within the research community, to monitor the variability at small scales, e.g., the mesoscale/submesoscale, to resolve the sub-basin/seasonal and inter-annual variability in the circulation, and thus establish the decadal variability, understand and correct the model-associated biases and to enhance model-data integration and ensemble forecasting for uncertainty estimation. Better knowledge and understanding of the level ofMediterranean variability will enable a subsequent evaluation of the impacts and mitigation of the effect of human activities and climate change on the biodiversity and the ecosystem, which will support environmental assessments and decisions. Further challenges include extending the science-based added-value products into societal relevant downstream services and engaging with communities to build initiatives that will contribute to the 2030 Agenda and more specifically to SDG14 and the UN’s Decade of Ocean Science for sustainable development, by this contributing to bridge the science-policy gap. The Mediterranean observing and forecasting capacity was built on the basis of community best practices in monitoring and modeling, and can serve as a basis for the development of an integrated global ocean observing system.

Published
2019

26. Steeper size spectra with decreasing phytoplankton biomass indicate strong trophic amplification and future fish declines.

Author

Atkinson A, Rossberg AG, Gaedke U, Sprules G, Heneghan RF, Batziakas S, Grigoratou M, Fileman E, Schmidt K, and Frangoulis C

Subjects
Animals, Biomass, Carbon, Phytoplankton, Nutritional Status, Nutrients
Abstract

Under climate change, model ensembles suggest that declines in phytoplankton biomass amplify into greater reductions at higher trophic levels, with serious implications for fisheries and carbon storage. However, the extent and mechanisms of this trophic amplification vary greatly among models, and validation is problematic. In situ size spectra offer a novel alternative, comparing biomass of small and larger organisms to quantify the net efficiency of energy transfer through natural food webs that are already challenged with multiple climate change stressors. Our global compilation of pelagic size spectrum slopes supports trophic amplification empirically, independently from model simulations. Thus, even a modest (16%) decline in phytoplankton this century would magnify into a 38% decline in supportable biomass of fish within the intensively-fished mid-latitude ocean. We also show that this amplification stems not from thermal controls on consumers, but mainly from temperature or nutrient controls that structure the phytoplankton baseline of the food web. The lack of evidence for direct thermal effects on size structure contrasts with most current thinking, based often on more acute stress experiments or shorter-timescale responses. Our synthesis of size spectra integrates these short-term dynamics, revealing the net efficiency of food webs acclimating and adapting to climatic stressors., (© 2024. The Author(s).)

Published
2024
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27. Responses of Free-Living Planktonic Bacterial Communities to Experimental Acidification and Warming.

Author

Tsiola A, Krasakopoulou E, Daffonchio D, Frangoulis C, Tsagaraki TM, Fodelianakis S, and Pitta P

Abstract

Climate change driven by human activities encompasses the increase in atmospheric CO 2 concentration and sea-surface temperature. Little is known regarding the synergistic effects of these phenomena on bacterial communities in oligotrophic marine ecosystems that are expected to be particularly vulnerable. Here, we studied bacterial community composition changes based on 16S rRNA sequencing at two fractions (0.1-0.2 and >0.2 μm) during a 10- day fully factorial mesocosm experiment in the eastern Mediterranean where the pH decreased by ~0.3 units and temperature increased by ~3 °C to project possible future changes in surface waters. The bacterial community experienced significant taxonomic differences driven by the combined effect of time and treatment; a community shift one day after the manipulations was noticed, followed by a similar state between all mesocosms at the third day, and mild shifts later on, which were remarkable mainly under sole acidification. The abundance of Synechococcus increased in response to warming, while the SAR11 clade immediately benefited from the combined acidification and warming. The effect of the acidification itself had a more persistent impact on community composition. This study highlights the importance of studying climate change consequences on ecosystem functioning both separately and simultaneously, considering the ambient environmental parameters.

Published
2023
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28. Methods and Best Practice to Intercompare Dissolved Oxygen Sensors and Fluorometers/Turbidimeters for Oceanographic Applications.

Author

Pensieri S, Bozzano R, Schiano ME, Ntoumas M, Potiris E, Frangoulis C, Podaras D, and Petihakis G

Abstract

In European seas, ocean monitoring strategies in terms of key parameters, space and time scale vary widely for a range of technical and economic reasons. Nonetheless, the growing interest in the ocean interior promotes the investigation of processes such as oxygen consumption, primary productivity and ocean acidity requiring that close attention is paid to the instruments in terms of measurement setup, configuration, calibration, maintenance procedures and quality assessment. To this aim, two separate hardware and software tools were developed in order to test and simultaneously intercompare several oxygen probes and fluorometers/turbidimeters, respectively in the same environmental conditions, with a configuration as close as possible to real in-situ deployment. The chamber designed to perform chlorophyll-a and turbidity tests allowed for the simultaneous acquisition of analogue and digital signals of several sensors at the same time, so it was sufficiently compact to be used in both laboratory and onboard vessels. Methodologies and best practice committed to the intercomparison of dissolved oxygen sensors and fluorometers/turbidimeters have been used, which aid in the promotion of interoperability to access key infrastructures, such as ocean observatories and calibration facilities. Results from laboratory tests as well as field tests in the Mediterranean Sea are presented.

Published
2016
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29. Comparison of marine copepod outfluxes: nature, rate, fate and role in the carbon and nitrogen cycles.

Author

Frangoulis C, Christou ED, and Hecq JH

Subjects
Animals, Feces chemistry, Respiration, Seawater, Carbon metabolism, Copepoda metabolism, Ecosystem, Nitrogen metabolism
Abstract

We compare the nature of copepod outfluxes of nonliving matter, the factors controlling their rate and their fate, and finally their role, particularly their relative importance in the carbon and nitrogen cycle. Copepods release dissolved matter through excretion and respiration and particulate matter through production of faecal pellets, carcasses, moults, and dead eggs. Excretion liberates several organic C, N, and P compounds and inorganic N and P compounds, with inorganic compounds constituting the larger part. The faecal pellets of copepods are covered by a peritrophic membrane and have a highly variable size and content. There is less information on the nature of other copepod particulate products. The weight-specific rates of posthatch mortality, respiration, excretion, and faecal pellet production have similar C or N levels and are higher than those of moulting and egg mortality. In general, most important factors controlling these rates are temperature, body mass, food concentration, food quality, and faunistic composition. Physical and biological factors govern the vertical fate of copepod products by affecting their sedimentation speed and concentration gradient. The physical factors are sinking speed, advection, stratification, turbulent diffusion, and molecular diffusion. They influence the sedimentation speed and degradation of the copepod products. The biological factors are production, biodegradation (by zooplankton, nekton, and microorganisms) and vertical migration of copepods (diel or seasonal). Physical degradation and biodegradation by zooplankton and nekton are faster than biodegradation by microorganisms. The most important copepod outfluxes are excretion and faecal pellet production. Excretion offers inorganic nutrients that can be directly used by primary producers. Faecal pellets have a more important role in the vertical transport of elements than the other particulate products. Most investigation has focused on carbon burial in the form of copepod faecal pellets, measured by sediment traps, and on the role of ammonia excretion in nutrient recycling. Full evaluation of the role of copepod products in the transport and recycling of elements and compounds requires a quantification of all copepod products and their different fates, particularly detritiphagy, remineralization, and integration as marine snow.

Published
2005
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30. [Adamantiades-Behcet syndrome. Current concepts].

Author

Georgiadès G, Sinakos Z, Frangoulis C, Psilas K, Economou T, Karyofillis K, and Kambouroglou J

Subjects
Adolescent, Adult, Behcet Syndrome pathology, Behcet Syndrome therapy, Female, Humans, Male, Middle Aged, Behcet Syndrome diagnosis
Published
1976

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