118 results on '"Frangoulidis D"'
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2. Biologische Bedrohung
- Author
-
Frangoulidis, D., Neitzel, Christian, editor, and Ladehof, Karsten, editor
- Published
- 2012
- Full Text
- View/download PDF
3. Bioterrorismus, infektiologische Aspekte
- Author
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Tomaso, H., Finke, E. -J, Frangoulidis, D, Darai, Gholamreza, editor, Handermann, Michaela, editor, Sonntag, Hans-Günther, editor, Tidona, Christian A., editor, and Zöller, Lothar, editor
- Published
- 2009
- Full Text
- View/download PDF
4. Coxiella burnetii (Q fever) as a cause of community-acquired pneumonia during the warm season in Germany
- Author
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SCHACK, M., SACHSE, S., RÖDEL, J., FRANGOULIDIS, D., PLETZ, M. W., ROHDE, G. U., STRAUBE, E., and BODEN, K.
- Published
- 2014
5. Übungsszenario für eine hochkontagiöse, lebensbedrohliche Erkrankung in der interkontinentalen Personenluftfahrt: Eine Kasuistik vor dem Hintergrund der internationalen Gesundheitsvorschriften (IGV)
- Author
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Stich, Heribert, Guggemos, W., Mühlhaus, A., Wicklein, B., Dietl, J., Hoffmann, A., Leiwering, J., Frangoulidis, D., Zange, S., Königstein, B., and Ippisch, S.
- Published
- 2015
- Full Text
- View/download PDF
6. First isolation of Coxiella burnetii from clinical material by cell-free medium (ACCM2)
- Author
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Boden, K., Wolf, K., Hermann, B., and Frangoulidis, D.
- Published
- 2015
- Full Text
- View/download PDF
7. Tularemia in Germany: The Tip of the Iceberg?
- Author
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EMIL Study Group, Splettstoesser, W. D., Piechotowski, I., Buckendahl, A., Frangoulidis, D., Kaysser, P., Kratzer, W., Kimmig, P., Seibold, E., and Brockmann, S. O.
- Published
- 2009
- Full Text
- View/download PDF
8. Bakterien- und Pilzgenom-Nachweis PCR/NAT: Auswertung des Ringversuchs November 2020 von INSTAND e.V. zur externen Qualitätskontrolle molekularbiologischer Nachweisverfahren in der bakteriologischen Diagnostik
- Author
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Reischl, U, Ehrenschwender, M, Hiergeist, A, Maaß, M, Baier, M, Frangoulidis, D, Grass, G, von Buttlar, H, Ehmann, R, Scholz, H, Fingerle, V, Sing, A, Dumke, R, Reiter-Owona, I, and Anders, A
- Subjects
ddc: 610 ,610 Medical sciences ,Medicine - Abstract
This contribution provides an analysis report of the recent proficiency testing scheme “Bacterial and fungal genome detection (PCR/NAT)”. It summarizes some benchmarks and the overall assessment of results reported by all of the participating laboratories. A highly desired scheme for external quality assessment schemes (EQAS) of molecular diagnostic methods in the field of medical microbiology was activated in 2002 by the German Society of Hygiene and Microbiology (DGHM) and is now organized by INSTAND e.V., Düsseldorf, Germany. This segment of the INSTAND e.V. proficiency testing program is open for diagnostic laboratories worldwide. The concept of this EQAS scheme, which is in accordance to the German RiLiBÄK, part B3, is based on two validation rounds per year (spring and autumn) and a permanently expanding coverage of relevant bacterial or fungal pathogens. Briefly, next to “simply negative” samples, the corresponding sets of QC specimens may contain some strong-positive samples, samples spiked with clinical variants or species closely related to the target organisms. Further information as well as the statistically documented and discussed results of the past rounds of this proficiency testing scheme “Bacterial and fungal genome detection (PCR/NAT)” can be found at the INSTAND e.V. website ([link:https://www.instand-ev.de*https://www.instand-ev.de]). Although the preferred language of these documents is German, we aim to provide at least a brief discussion of the results and some key issues in English and keep the tables in a bilingual style., Der vorliegende Beitrag liefert einen Auswertungsbericht der jüngsten Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis PCR/NAT“. Er fasst die Zielwerte, einige Bezugsgrößen und die Gesamtbewertung der Ergebnisse aller teilnehmenden Laboratorien zusammen. Diese hochwillkommene Versuchsreihe zur externen Qualitätskontrolle (EQAS; external quality assessment scheme) von Methoden der molekularen Diagnostik auf dem Gebiet der medizinischen Mikrobiologie wurde 2002 von der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) angestoßen und wird seither von INSTAND e.V., Düsseldorf, organisiert. Dieses Segment der INSTAND e.V.-Ringversuchsserie wird für diagnostische Laboratorien weltweit angeboten. Unser Ringversuchskonzept entspricht der aktuellen Richtlinie der Bundesärztekammer zur Qualitätssicherung laboratoriumsmedizinischer Untersuchungen (RiLiBÄK), Teil B3, und basiert auf zwei Validierungsrunden pro Jahr (im Frühjahr und Herbst) unter einer permanent wachsenden Abdeckung der relevanten bakteriellen und fungalen humanpathogenen Erreger. Die entsprechenden Sets von Quality Control (QC)-Proben können dabei neben negativen Proben auch einige stark-positive Proben, Proben mit klinischen Varianten oder eng mit den Zielorganismen verwandte Spezies oder klinische Isolate enthalten. Weitergehende Informationen sowie die statistisch aufgearbeiteten und dokumentierten Ergebnisse der vergangenen Runden dieser Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis (PCR/NAT)“ können auf der Webseite von INSTAND e.V. ([link:https://www.instand-ev.de*https://www.instand-ev.de]) eingesehen werden. Obwohl die bevorzugte Sprache dieser Dokumente Deutsch ist, streben wir an, zumindest eine kurze Diskussion der Ergebnisse sowie die wichtigsten wissenschaftlichen Aspekte in Englisch bereitzustellen und die Tabellen zweisprachig zu gestalten., GMS Zeitschrift zur Förderung der Qualitätssicherung in medizinischen Laboratorien; 12:Doc02
- Published
- 2021
- Full Text
- View/download PDF
9. MLVA and SNP analysis identified a unique genetic cluster in Bulgarian Bacillus anthracis strains
- Author
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Antwerpen, M., Ilin, D., Georgieva, E., Meyer, H., Savov, E., and Frangoulidis, D.
- Published
- 2011
- Full Text
- View/download PDF
10. Bakterien- und Pilzgenom-Nachweis PCR/NAT: Auswertung des Ringversuchs November 2019 von INSTAND e.V. zur externen Qualitätskontrolle molekularbiologischer Nachweisverfahren in der bakteriologischen Diagnostik
- Author
-
Reischl, U, Ehrenschwender, M, Hiergeist, A, Maaß, M, Baier, M, Frangoulidis, D, Grass, G, von Buttlar, H, Scholz, H, Fingerle, V, Sing, A, Dumke, R, Reiter-Owona, I, and Anders, A
- Subjects
ddc: 610 - Abstract
This contribution provides an analysis report of the recent proficiency testing scheme "Bacterial and Fungal Genome Detection (PCR/NAT)". It summarizes some benchmarks and the overall assessment of results reported by all of the participating laboratories.A highly desired scheme for external quality assessment schemes (EQAS) of molecular diagnostic methods in the field of medical microbiology was activated in 2002 by the German Society of Hygiene and Microbiology (DGHM) and is now organized by INSTAND e.V., Düsseldorf, Germany. This segment of the INSTAND e.V. proficiency testing program is open for diagnostic laboratories worldwide. The concept of this EQAS scheme, which is in accordance to the German RiLiBÄK, part B3, is based on two validation rounds per year (spring and autumn) and a permanently expanding coverage of relevant bacterial or fungal pathogens.Briefly, next to "simply negative" samples, the corresponding sets of QC specimens may contain some strong-positive samples, samples spiked with clinical variants or species closely related to the target organisms. Further information as well as the statistically documented and discussed results of the past rounds of this proficiency testing scheme "Bacterial and Fungal Genome Detection (PCR/NAT)" can be found at the INSTAND e.V. website (https://www.instand-ev.de). Although the preferred language of these documents is German, we aim to provide at least a brief discussion of the results and some key issues in English and keep the tables in a bilingual style. Der vorliegende Beitrag liefert einen Auswertungsbericht der jüngsten Ringversuchsserie "Bakterien- und Pilzgenom-Nachweis PCR/NAT". Er fasst die Zielwerte, einige Bezugsgrößen und die Gesamtbewertung der Ergebnisse aller teilnehmenden Laboratorien zusammen.Diese hochwillkommene Versuchsreihe zur externen Qualitätskontrolle (EQAS; external quality assessment scheme ) von Methoden der molekularen Diagnostik auf dem Gebiet der medizinischen Mikrobiologie wurde 2002 von der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) angestoßen und wird seither von Instand e.V., Düsseldorf, organisiert. Dieses Segment der INSTAND e.V.-Ringversuchsserie wird für diagnostische Laboratorien weltweit angeboten. Unser Ringversuchskonzept entspricht der aktuellen Richtlinie der Bundesärztekammer zur Qualitätssicherung laboratoriumsmedizinischer Untersuchungen (RiLiBÄK), Teil B3, und basiert auf zwei Validierungsrunden pro Jahr (im Frühjahr und Herbst) unter einer permanent wachsenden Abdeckung der relevanten bakteriellen und fungalen humanpathogenen Erreger. Die entsprechenden Sets von Quality Control (QC)-Proben können dabei neben negativen Proben auch einige stark-positive Proben, Proben mit klinischen Varianten oder eng mit den Zielorganismen verwandte Spezies oder klinische Isolate enthalten. Weitergehende Informationen sowie die statistisch aufgearbeiteten und dokumentierten Ergebnisse der vergangenen Runden dieser Ringversuchsserie "Bakterien- und Pilzgenom-Nachweis (PCR/NAT)" können auf der Webseite von Instand e.V. (https://www.instand-ev.de) eingesehen werden. Obwohl die bevorzugte Sprache dieser Dokumente Deutsch ist, streben wir an, zumindest eine kurze Diskussion der Ergebnisse sowie die wichtigsten wissenschaftlichen Aspekte in Englisch bereitzustellen und die Tabellen zweisprachig zu gestalten.
- Published
- 2020
11. Bakterien- und Pilzgenom-Nachweis PCR/NAT: Auswertung des Ringversuchs Juni 2019 von INSTAND e.V. zur externen Qualitätskontrolle molekularbiologischer Nachweisverfahren in der bakteriologischen Diagnostik
- Author
-
Reischl, U, Ehrenschwender, M, Hiergeist, A, Maaß, M, Baier, M, Frangoulidis, D, Grass, G, von Buttlar, H, Scholz, H, Fingerle, V, Sing, A, Dumke, R, Reiter-Owona, I, and Anders, A
- Subjects
ddc: 610 ,610 Medical sciences ,Medicine - Abstract
This contribution provides an analysis report of the recent proficiency testing scheme “Bacterial and Fungal Genome Detection (PCR/NAT)”. It summarizes some benchmarks and the overall assessment of results reported by all of the participating laboratories. A highly desired scheme for external quality assessment schemes (EQAS) of molecular diagnostic methods in the field of medical microbiology was activated in 2002 by the German Society of Hygiene and Microbiology (DGHM) and is now organized by INSTAND e.V., Düsseldorf, Germany. This segment of the INSTAND e.V. proficiency testing program is open for diagnostic laboratories worldwide. The concept of this EQAS scheme, which is in accordance to the German RiLiBÄK, part B3, is based on two validation rounds per year (spring and autumn) and a permanently expanding coverage of relevant bacterial or fungal pathogens. Briefly, next to “simply negative” samples, the corresponding sets of QC specimens may contain some strong-positive samples, samples spiked with clinical variants or species closely related to the target organisms. Further information as well as the statistically documented and discussed results of the past rounds of this proficiency testing scheme “Bacterial and Fungal Genome Detection (PCR/NAT)” can be found at the INSTAND e.V. website ([link:https://www.instand-ev.de*https://www.instand-ev.de]). Although the preferred language of these documents is German, we aim to provide at least a brief discussion of the results and some key issues in English and keep the tables in a bilingual style., Der vorliegende Beitrag liefert einen Auswertungsbericht der jüngsten Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis PCR/NAT“. Er fasst die Zielwerte, einige Bezugsgrößen und die Gesamtbewertung der Ergebnisse aller teilnehmenden Laboratorien zusammen. Diese hochwillkommene Versuchsreihe zur externen Qualitätskontrolle (EQAS; external quality assessment scheme) von Methoden der molekularen Diagnostik auf dem Gebiet der medizinischen Mikrobiologie wurde 2002 von der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) angestoßen und wird seither von Instand e.V., Düsseldorf, organisiert. Dieses Segment der INSTAND e.V.-Ringversuchsserie wird für diagnostische Laboratorien weltweit angeboten. Unser Ringversuchskonzept entspricht der aktuellen Richtlinie der Bundesärztekammer zur Qualitätssicherung laboratoriumsmedizinischer Untersuchungen (RiLiBÄK), Teil B3, und basiert auf zwei Validierungsrunden pro Jahr (im Frühjahr und Herbst) unter einer permanent wachsenden Abdeckung der relevanten bakteriellen und fungalen humanpathogenen Erreger. Die entsprechenden Sets von Quality Control (QC)-Proben können dabei neben negativen Proben auch einige stark-positive Proben, Proben mit klinischen Varianten oder eng mit den Zielorganismen verwandte Spezies oder klinische Isolate enthalten. Weitergehende Informationen sowie die statistisch aufgearbeiteten und dokumentierten Ergebnisse der vergangenen Runden dieser Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis (PCR/NAT)“ können auf der Webseite von Instand e.V. ([link:https://www.instand-ev.de*https://www.instand-ev.de]) eingesehen werden. Obwohl die bevorzugte Sprache dieser Dokumente Deutsch ist, streben wir an, zumindest eine kurze Diskussion der Ergebnisse sowie die wichtigsten wissenschaftlichen Aspekte in Englisch bereitzustellen und die Tabellen zweisprachig zu gestalten., GMS Zeitschrift zur Förderung der Qualitätssicherung in medizinischen Laboratorien; 11:Doc01
- Published
- 2020
- Full Text
- View/download PDF
12. A monoclonal antibody specific for a unique biomarker, virenose, in a lipopolysaccharide of Coxiella burnetii
- Author
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Palkovicova, K., Ihnatko, R., Vadovic, P., Betinova, E., Skultety, L., Frangoulidis, D., and Toman, R.
- Published
- 2009
- Full Text
- View/download PDF
13. DNA microarray-chip based diagnosis of Q-fever (Coxiella burnetii)
- Author
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Frangoulidis, D., Rodolakis, A., Heiser, V., Landt, O., Splettstoesser, W., and Meyer, H.
- Published
- 2009
- Full Text
- View/download PDF
14. Tularemia in Germany: the tip of the iceberg?
- Author
-
SPLETTSTOESSER, W. D., PIECHOTOWSKI, I., BUCKENDAHL, A., FRANGOULIDIS, D., KAYSSER, P., KRATZER, W., KIMMIG, P., SEIBOLD, E., and BROCKMANN, S. O.
- Published
- 2009
15. Bakterien- und Pilzgenom-Nachweis PCR/NAT: Auswertung des Ringversuchs Juni 2018 von INSTAND e.V. zur externen Qualitätskontrolle molekularbiologischer Nachweisverfahren in der bakteriologischen Diagnostik
- Author
-
Reischl, U, Ehrenschwender, M, Hiergeist, A, Maaß, M, Baier, M, Frangoulidis, D, Grass, G, von Buttlar, H, Scholz, H, Fingerle, V, Sing, A, Dumke, R, Reiter-Owona, I, and Anders, A
- Subjects
ddc: 610 ,610 Medical sciences ,Medicine - Abstract
This contribution provides an analysis report of the recent proficiency testing scheme “Bacterial and Fungal Genome Detection (PCR/NAT)”. It summarizes some benchmarks and the overall assessment of results reported by all of the participating laboratories. A highly desired scheme for external quality assessment (EQAS) of molecular diagnostic methods in the field of medical microbiology was activated in 2002 by the German Society of Hygiene and Microbiology (DGHM) and is now organized by INSTAND e.V., Düsseldorf, Germany. This segment of the INSTAND e.V. proficiency testing program is open for diagnostic laboratories worldwide. The concept of this EQAS scheme, which is in accordance to the German RiLiBÄK, part B3, is based on two validation rounds per year (spring and autumn) and a permanently expanding coverage of relevant bacterial or fungal pathogens. Briefly, next to “simply negative” samples the corresponding sets of QC specimens may contain some strong-positive samples, samples spiked with clinical variants or species closely related to the target organisms. Further information as well as the statistically documented and discussed results of the past rounds of this proficiency testing scheme “Bacterial and Fungal Genome Detection (PCR/NAT)” can be found at the homepage of INSTAND e.V. ([link:http://www.instand-ev.de*http://www.instand-ev.de]). Although the preferred language of these documents is German, we are aiming to provide at least a brief discussion of the results and some key issues in English and keep the tables in a bilingual style., Der vorliegende Beitrag liefert einen Auswertungsbericht der jüngsten Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis PCR/NAT“. Er fasst die Zielwerte, einige Bezugsgrößen und die Gesamtbewertung der Ergebnisse aller teilnehmenden Laboratorien zusammen. Diese hochwillkommene Versuchsreihe zur externen Qualitätskontrolle (EQAS; external quality assessment scheme) von Methoden der molekularen Diagnostik auf dem Gebiet der medizinischen Mikrobiologie wurde 2002 von der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) angestoßen und wird seither von Instand e.V., Düsseldorf, organisiert. Dieses Segment der INSTAND e.V.-Ringversuchsserie wird für diagnostische Laboratorien weltweit angeboten. Unser Ringversuchskonzept entspricht der aktuellen Richtlinie der Bundesärztekammer zur Qualitätssicherung laboratoriumsmedizinischer Untersuchungen (RiLiBÄK), Teil B3, und basiert auf zwei Validierungsrunden pro Jahr (im Frühjahr und Herbst) unter einer permanent wachsenden Abdeckung der relevanten bakteriellen und fungalen humanpathogenen Erreger. Die entsprechenden Sets von Quality Control (QC)-Proben können dabei neben negativen Proben auch einige stark-positive Proben, Proben mit klinischen Varianten oder eng mit den Zielorganismen verwandte Spezies oder klinische Isolate enthalten. Weitergehende Informationen sowie die statistisch aufgearbeiteten und dokumentierten Ergebnisse der vergangenen Runden dieser Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis (PCR/NAT)“ können auf der Homepage von Instand e.V. ([link:http://www.instand-ev.de*http://www.instand-ev.de]) eingesehen werden. Obwohl die bevorzugte Sprache dieser Dokumente deutsch ist, streben wir an, zumindest eine kurze Diskussion der Ergebnisse sowie die wichtigsten wissenschaftlichen Aspekte in Englisch bereitzustellen und die Tabellen zweisprachig zu gestalten., GMS Zeitschrift zur Förderung der Qualitätssicherung in medizinischen Laboratorien; 10:Doc01
- Published
- 2019
- Full Text
- View/download PDF
16. Immune electron-microscopy study of the trimeric glycoprotein (CD 103) of hairy cell leukemia using the Ber-ACT8 monoclonal antibody
- Author
-
Frangoulidis, D., Schulz, A., and Pralle, H.
- Published
- 1994
- Full Text
- View/download PDF
17. Bakterien- und Pilzgenom-Nachweis PCR/NAT: Auswertung des Ringversuchs Juni 2017 von INSTAND e.V. zur externen Qualitätskontrolle molekularbiologischer Nachweisverfahren in der bakteriologischen Diagnostik
- Author
-
Reischl, U, Schneider, W, Ehrenschwender, M, Hiergeist, A, Maaß, M, Baier, M, Frangoulidis, D, Grass, G, von Buttlar, H, Scholz, H, Fingerle, V, Sing, A, Jacobs, E, Reiter-Owona, I, and Anders, A
- Subjects
ddc: 610 ,610 Medical sciences ,Medicine - Abstract
This contribution provides an analysis report of the recent proficiency testing scheme “Bacterial and Fungal Genome Detection (PCR/NAT)”. It summarizes some benchmarks and the overall assessment of results reported by all of the participating laboratories. A highly desired scheme for external quality assessment (EQAS) of molecular diagnostic methods in the field of medical microbiology was activated in 2002 by the German Society of Hygiene and Microbiology (DGHM) and is now organized by INSTAND e.V., Düsseldorf, Germany. This segment of the INSTAND e.V. proficiency testing program is open for diagnostic laboratories worldwide. The concept of this EQAS scheme, which is in accordance to the German RiLiBÄK, part B3, is based on two validation rounds per year (spring and autumn) and a permanently expanding coverage of relevant bacterial or fungal pathogens. Briefly, next to “simply negative” samples the corresponding sets of QC specimens may contain some strong-positive samples, samples spiked with clinical variants or species closely related to the target organisms. Further information as well as the statistically documented and discussed results of the past rounds of this proficiency testing scheme “Bacterial and Fungal Genome Detection (PCR/NAT)” can be found at the homepage of INSTAND e.V. ([link:http://www.instand-ev.de*http://www.instand-ev.de]). Although the preferred language of these documents is German, we are aiming to provide at least a brief discussion of the results and some key issues in English and keep the tables in a bilingual style., Der vorliegende Beitrag liefert einen Auswertungsbericht der jüngsten Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis PCR/NAT“. Er fasst die Zielwerte, einige Bezugsgrößen und die Gesamtbewertung der Ergebnisse aller teilnehmenden Laboratorien zusammen. Diese hochwillkommene Versuchsreihe zur externen Qualitätskontrolle (EQAS; external quality assessment scheme) von Methoden der molekularen Diagnostik auf dem Gebiet der medizinischen Mikrobiologie wurde 2002 von der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) angestoßen und wird seither von Instand e.V., Düsseldorf, organisiert. Dieses Segment der INSTAND e.V.-Ringversuchsserie wird für diagnostische Laboratorien weltweit angeboten. Unser Ringversuchskonzept entspricht der aktuellen Richtlinie der Bundesärztekammer zur Qualitätssicherung laboratoriumsmedizinischer Untersuchungen (RiLiBÄK), Teil B3, und basiert auf zwei Validierungsrunden pro Jahr (im Frühjahr und Herbst) unter einer permanent wachsenden Abdeckung der relevanten bakteriellen und fungalen humanpathogenen Erreger. Die entsprechenden Sets von Quality Control (QC)-Proben können dabei neben negativen Proben auch einige stark-positive Proben, Proben mit klinischen Varianten oder eng mit den Zielorganismen verwandte Spezies oder klinische Isolate enthalten. Weitergehende Informationen sowie die statistisch aufgearbeiteten und dokumentierten Ergebnisse der vergangenen Runden dieser Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis (PCR/NAT)“ können auf der Homepage von Instand e.V. ([link:http://www.instand-ev.de*http://www.instand-ev.de]) eingesehen werden. Obwohl die bevorzugte Sprache dieser Dokumente deutsch ist, streben wir an, zumindest eine kurze Diskussion der Ergebnisse sowie die wichtigsten wissenschaftlichen Aspekte in Englisch bereitzustellen und die Tabellen zweisprachig zu gestalten., GMS Zeitschrift zur Förderung der Qualitätssicherung in medizinischen Laboratorien; 8:Doc03
- Published
- 2017
- Full Text
- View/download PDF
18. Cerebellitis from infection with Coxiella burnettii
- Author
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Mollenhauer, M, Strickler, A, Frangoulidis, D, Gardill, K, Schneider, R, and Isenhardt, K
- Published
- 2024
- Full Text
- View/download PDF
19. Bakterien- und Pilzgenom-Nachweis PCR/NAT: Auswertung des Ringversuchs Mai 2016 von INSTAND e.V. zur externen Qualitätskontrolle molekularbiologischer Nachweisverfahren in der bakteriologischen Diagnostik
- Author
-
Reischl, U, Schneider, W, Ehrenschwender, M, Hiergeist, A, Maaß, M, Baier, M, Straube, E, Frangoulidis, D, Grass, G, von Buttlar, H, Fingerle, V, Sing, A, Jacobs, E, Reiter-Owona, I, Anders, A, and Kaase, M
- Subjects
ddc: 610 ,610 Medical sciences ,Medicine - Abstract
This contribution provides an analysis report of the recent proficiency testing scheme “Bacterial and Fungal Genome Detection (PCR/NAT)”. It summarizes some benchmarks and the overall assessment of results reported by all of the participating laboratories. A highly desired scheme for external quality assessment (EQAS) of molecular diagnostic methods in the field of medical microbiology was activated in 2002 by the German Society of Hygiene and Microbiology (DGHM) and is now organized by INSTAND e.V., Düsseldorf, Germany. This segment of the INSTAND e.V. proficiency testing program is open for diagnostic laboratories worldwide. The concept of this EQAS scheme, which is in accordance to the German RiLiBÄK, part B3, is based on two validation rounds per year (spring and autumn) and a permanently expanding coverage of relevant bacterial or fungal pathogens. Briefly, next to “simply negative” samples the corresponding sets of QC specimens may contain some strong-positive samples, samples spiked with clinical variants or species closely related to the target organisms. Further information as well as the statistically documented and discussed results of the past rounds of this proficiency testing scheme “Bacterial and Fungal Genome Detection (PCR/NAT)” can be found at the homepage of INSTAND e.V. ([link:http://www.instand-ev.de*http://www.instand-ev.de]). Although the preferred language of these documents is German, we are aiming to provide at least a brief discussion of the results and some key issues in English and keep the tables in a bilingual style., Der vorliegende Beitrag liefert einen Auswertungsbericht der jüngsten Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis PCR/NAT“. Er fasst die Zielwerte, einige Bezugsgrößen und die Gesamtbewertung der Ergebnisse aller teilnehmenden Laboratorien zusammen. Diese hochwillkommene Versuchsreihe zur externen Qualitätskontrolle (EQAS; external quality assessment scheme) von Methoden der molekularen Diagnostik auf dem Gebiet der medizinischen Mikrobiologie wurde 2002 von der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) angestoßen und wird seither von Instand e.V., Düsseldorf, organisiert. Dieses Segment der INSTAND e.V.-Ringversuchsserie wird für diagnostische Laboratorien weltweit angeboten. Unser Ringversuchskonzept entspricht der aktuellen Richtlinie der Bundesärztekammer zur Qualitätssicherung laboratoriumsmedizinischer Untersuchungen (RiLiBÄK), Teil B3, und basiert auf zwei Validierungsrunden pro Jahr (im Frühjahr und Herbst) unter einer permanent wachsenden Abdeckung der relevanten bakteriellen und fungalen humanpathogenen Erreger. Die entsprechenden Sets von Quality Control (QC)-Proben können dabei neben negativen Proben auch einige stark-positive Proben, Proben mit klinischen Varianten oder eng mit den Zielorganismen verwandte Spezies oder klinische Isolate enthalten. Weitergehende Informationen sowie die statistisch aufgearbeiteten und dokumentierten Ergebnisse der vergangenen Runden dieser Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis (PCR/NAT)“ können auf der Homepage von Instand e.V. ([link:http://www.instand-ev.de*http://www.instand-ev.de]) eingesehen werden. Obwohl die bevorzugte Sprache dieser Dokumente deutsch ist, streben wir an, zumindest eine kurze Diskussion der Ergebnisse sowie die wichtigsten wissenschaftlichen Aspekte in Englisch bereitzustellen und die Tabellen zweisprachig zu gestalten., GMS Zeitschrift zur Förderung der Qualitätssicherung in medizinischen Laboratorien; 7:Doc03
- Published
- 2016
- Full Text
- View/download PDF
20. Bioterrorismus, infektiologische Aspekte
- Author
-
Tomaso, H., Finke, E. -J, and Frangoulidis, D
- Subjects
Article - Abstract
Zusammenfassung Infektionskrankheiten sind ständige Begleiter und gefürchtete Geißeln der Menschheit. Pest und Pocken versetzen als todbringende Seuchen die Menschen nicht erst seit dem Altertum in Schrecken (lat.: terror). Archaische Ängste und vor allem eine hohe Medienaufmerksamkeit sorgen immer wieder für Panik und irrationale Reaktionen: Im indischen Surat setzte im Herbst 1994 während eines ungewöhnlichen Pestausbruchs eine Massenflucht ein, nachdem die Presse den Verdacht auf Lungenpest und terroristische Anschläge verbreitet hatte. Über 800.000 Menschen, darunter auch zahlreiche Ärzte und Pflegekräfte, verließen daraufhin ihre Arbeitsplätze und Wohnorte. Allein die drastischen Flug- und Handelsbeschränkungen brachten Indien einen ökonomischen Schaden von etwa 3 Milliarden US $.
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- 2011
21. Bakterien- und Pilzgenom-Nachweis PCR/NAT: Auswertung des Ringversuchs November 2014 von INSTAND e.V. zur externen Qualitätskontrolle molekularbiologischer Nachweisverfahren in der bakteriologischen Diagnostik
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Reischl, U, Schneider, W, Holzmann, T, Ehrenschwender, M, Hiergeist, A, Maaß, M, Straube, E, Frangoulidis, D, Grass, G, Splettstößer, W, Fingerle, V, Sing, A, Jacobs, E, Reiter-Owona, I, and Kaase, M
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ddc: 610 ,610 Medical sciences ,Medicine - Abstract
Der vorliegende Beitrag liefert einen Auswertungsbericht der jüngsten Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis PCR/NAT“. Er fasst die Zielwerte, einige Bezugsgrößen und die Gesamtbewertung der Ergebnisse aller teilnehmenden Laboratorien zusammen. Diese hochwillkommene Versuchsreihe zur externen Qualitätskontrolle (EQAS, external quality assessment scheme) von Methoden der molekularen Diagnostik auf dem Gebiet der medizinischen Mikrobiologie wurde 2002 von der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) angestoßen und wird seither von Instand e.V., Düsseldorf organisiert. Dieses Segment der INSTAND e.V.-Ringversuchsserie wird für diagnostische Laboratorien weltweit angeboten. Unser Ringversuchskonzept entspricht der aktuellen Richtlinie der Bundesärztekammer zur Qualitätssicherung laboratoriumsmedizinischer Untersuchungen (RiLiBÄK), Teil B3, und basiert auf zwei Validierungsrunden pro Jahr (im Frühjahr und Herbst) unter einer permanent wachsenden Abdeckung der relevanten bakteriellen und fungalen humanpathogenen Erreger. Die entsprechenden Sets von Quality Control (QC)-Proben können dabei neben negativen Proben auch einige stark-positive Proben, Proben mit klinischen Varianten oder eng mit den Zielorganismen verwandte Spezies oder klinische Isolate enthalten. Weitergehende Informationen sowie die statistisch aufgearbeiteten und dokumentierten Ergebnisse der vergangenen Runden dieser Ringversuchsserie „Bakterien- und Pilzgenom-Nachweis (PCR/NAT)“ können auf der Homepage von Instand e.V. ([link:http://www.instandev.de*http://www.instandev.de]) eingesehen werden. Obwohl die bevorzugte Sprache dieser Dokumente deutsch ist, streben wir an, zumindest eine kurze Diskussion der Ergebnisse sowie die wichtigsten wissenschaftlichen Aspekte in Englisch bereitzustellen und die Tabellen zweisprachig zu gestalten., GMS Zeitschrift zur Förderung der Qualitätssicherung in medizinischen Laboratorien; 5:Doc05; ISSN 1869-4241
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- 2014
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22. Microarray-Chip based diagnosis of Q fever (Coxiella burnetii)
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Frangoulidis, D, Rodolakis, Annie, Heiser, V, Landt, O, Meyer, H, ProdInra, Migration, Inconnu, Infectiologie Animale et Santé Publique (UR IASP), and Institut National de la Recherche Agronomique (INRA)
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[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
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- 2008
23. Coxiella burnetii(Q fever) as a cause of community-acquired pneumonia during the warm season in Germany
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SCHACK, M., primary, SACHSE, S., additional, RÖDEL, J., additional, FRANGOULIDIS, D., additional, PLETZ, M. W., additional, ROHDE, G. U., additional, STRAUBE, E., additional, and BODEN, K., additional
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- 2013
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24. Fatal anthrax infection in a heroin user from southern Germany, June 2012
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Holzmann, T, primary, Frangoulidis, D, additional, Simon, M, additional, Noll, P, additional, Schmoldt, S, additional, Hanczaruk, M, additional, Grass, G, additional, Pregler, M, additional, Sing, A, additional, Hörmansdorfer, S, additional, Bernard, H, additional, Grunow, R, additional, Zimmermann, R, additional, Schneider-Brachert, W, additional, Gessner, A, additional, and Reischl, U, additional
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- 2012
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25. Cerebellitis from infection with Coxiella burnettii
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Mollenhauer, M, primary, Strickler, A, additional, Frangoulidis, D, additional, Gardill, K, additional, Schneider, R, additional, and Isenhardt, K, additional
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- 2009
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26. ‘Imported’ melioidosis in Germany: relapse after 10 years
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Frangoulidis, D., primary, Schwab, D., additional, Scholz, H., additional, Tomaso, H., additional, Hogardt, M., additional, Meyer, H., additional, Splettstoesser, W.D., additional, and Pohle, F.K., additional
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- 2008
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27. Tularemia in Germany: the tip of the iceberg?
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SPLETTSTOESSER, W. D., primary, PIECHOTOWSKI, I., additional, BUCKENDAHL, A., additional, FRANGOULIDIS, D., additional, KAYSSER, P., additional, KRATZER, W., additional, KIMMIG, P., additional, SEIBOLD, E., additional, and BROCKMANN, S. O., additional
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- 2008
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28. Seltene Zoonosen – eine unerkannte Bedrohung in Deutschland? Die Seroprävalenz von Q-Fieber, Tularämie und alveolärer Echinokokkose
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Brockmann, SO, primary, Splettstoesser, W, additional, Frangoulidis, D, additional, Kratzer, W, additional, Kimmig, P, additional, Meyer, H, additional, Bertling, U, additional, and Piechotowski, I, additional
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- 2007
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29. Comparison of Four Commercially Available Assays for the Detection of IgM Phase II Antibodies to Coxiella burnetii in the Diagnosis of Acute Q Fever
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FRANGOULIDIS, D., primary, SCHROPFER, E., additional, DAHOUK, S. A., additional, TOMASO, H., additional, and MEYER, H., additional
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- 2006
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30. Immunologic response of unvaccinated workers exposed to anthrax, Belgium.
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Wattiau P, Govaerts M, Frangoulidis D, Fretin D, Kissling E, Van Hessche M, China B, Poncin M, Pirenne Y, Hanquet G, Wattiau, Pierre, Govaerts, Marc, Frangoulidis, Dimitrios, Fretin, David, Kissling, Esther, Van Hessche, Mieke, China, Bernard, Poncin, Martine, Pirenne, Yvo, and Hanquet, Germaine
- Abstract
To determine immunologic reactivity to Bacillus anthrax antigens, we conducted serologic testing of workers in a factory that performed scouring of wool and goat hair. Of 66 workers, approximately 10% had circulating antibodies or T lymphocytes that reacted with anthrax protective antigen. Individual immunity varied from undetectable to high. [ABSTRACT FROM AUTHOR]
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- 2009
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31. Measures undertaken in the German Armed Forces Field Hospital deployed in Kosovo to contain a potential outbreak of Crimean-Congo hemorrhagic fever.
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Frangoulidis D, Meyer H, Frangoulidis, Dimitrios, and Meyer, Hermann
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During May and June 2001, the World Health Organization reported an outbreak of Crimean-Congo hemorrhagic fever in Kosovo. Two of the outbreak foci were located within the German Kosovo Force's zone of responsibility and thus countermeasures were undertaken at the German field hospital in Prizren to prepare for a potential outbreak among soldiers. A risk assessment was undertaken and the following essential issues were addressed: the education of troops with emphasis given to the prevention of tick bites, the establishment of an isolation unit, including barrier nursing and technical safety measures, the establishment of procedures for the safe handling of biological specimens intended for laboratory diagnosis, protocols for the treatment and prophylaxis with ribavirin, protocols for effective disinfection and decontamination, and an "outbreak plan" should the disease spread among soldiers. Using Army Mobile Field Hospital System equipment, a plan was implemented within 72 hours. The procedures described herein are likely to be suitable for the containment of other highly contagious diseases. [ABSTRACT FROM AUTHOR]
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- 2005
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32. Laboratory-based diagnosis of brucellosis - A review of the literature
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Sascha Al Dahouk, Tomaso, H., Nöckler, K., Neubauer, H., and Frangoulidis, D.
33. High throughput detection of Coxiella burnetii by real-time PCR with internal control system and automated DNA preparation
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Kramme Stefanie, Peters Martin, Greiner-Fischer Susanne, Kilwinski Jochen, Panning Marcus, Frangoulidis Dimitrios, Meyer Hermann, Henning Klaus, and Drosten Christian
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Microbiology ,QR1-502 - Abstract
Abstract Background Coxiella burnetii is the causative agent of Q-fever, a widespread zoonosis. Due to its high environmental stability and infectivity it is regarded as a category B biological weapon agent. In domestic animals infection remains either asymptomatic or presents as infertility or abortion. Clinical presentation in humans can range from mild flu-like illness to acute pneumonia and hepatitis. Endocarditis represents the most common form of chronic Q-fever. In humans serology is the gold standard for diagnosis but is inadequate for early case detection. In order to serve as a diagnostic tool in an eventual biological weapon attack or in local epidemics we developed a real-time 5'nuclease based PCR assay with an internal control system. To facilitate high-throughput an automated extraction procedure was evaluated. Results To determine the minimum number of copies that are detectable at 95% chance probit analysis was used. Limit of detection in blood was 2,881 copies/ml [95%CI, 2,188–4,745 copies/ml] with a manual extraction procedure and 4,235 copies/ml [95%CI, 3,143–7,428 copies/ml] with a fully automated extraction procedure, respectively. To demonstrate clinical application a total of 72 specimens of animal origin were compared with respect to manual and automated extraction. A strong correlation between both methods was observed rendering both methods suitable. Testing of 247 follow up specimens of animal origin from a local Q-fever epidemic rendered real-time PCR more sensitive than conventional PCR. Conclusion A sensitive and thoroughly evaluated real-time PCR was established. Its high-throughput mode may show a useful approach to rapidly screen samples in local outbreaks for other organisms relevant for humans or animals. Compared to a conventional PCR assay sensitivity of real-time PCR was higher after testing samples from a local Q-fever outbreak.
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- 2008
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34. Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing
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Meyer Hermann, Souriau Armel, Bodier Christelle C, Frangoulidis Dimitrios, Bejaoui Awatef, Hauck Yolande, Arricau-Bouvery Nathalie, Neubauer Heinrich, Rodolakis Annie, and Vergnaud Gilles
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Microbiology ,QR1-502 - Abstract
Abstract Background Coxiella burnetii, the causative agent of Q fever, has a wide host range. Few epidemiological tools are available, and they are often expensive or not easily standardized across laboratories. In this work, C. burnetii isolates from livestock and ticks were typed using infrequent restriction site-PCR (IRS-PCR) and multiple loci variable number of tandem repeats (VNTR) analysis (MLVA). Results By applying IRS-PCR, 14 C. burnetii isolates could be divided into six groups containing up to five different isolates. Clustering as deduced from MLVA typing with 17 markers provided an increased resolution with an excellent agreement to IRS-PCR, and with the plasmid type of each strain. MLVA was then applied to 28 additional C. burnetii isolates of different origin and 36 different genotypes were identified among the 42 isolates investigated. The clustering obtained is in agreement with published Multiple Locus Sequence Typing (MLST) data. Two panels of markers are proposed, panel 1 which can be confidently typed on agarose gel at a lower cost and in any laboratory setting (10 minisatellite markers with a repeat unit larger than 9 bp), and panel 2 which comprises 7 microsatellites and provides a higher discriminatory power. Conclusion Our analyses demonstrate that MLVA is a powerful and promising molecular typing tool with a high resolution and of low costs. The consistency of the results with independent methods suggests that MLVA can be applied for epidemiological studies. The resulting data can be queried on a dedicated MLVA genotyping Web service.
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- 2006
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35. Control of Coxiella burnetii shedding in a dairy goat herd by annual offspring vaccination.
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Bauer BU, Herms TL, Jahnke R, Ossowski N, Walter MC, Frangoulidis D, Runge M, Ganter M, and Knittler MR
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- Animals, Female, Milk immunology, Milk microbiology, Immunoglobulin G blood, Dairying, Germany, Goats, Coxiella burnetii immunology, Q Fever prevention & control, Q Fever immunology, Q Fever veterinary, Bacterial Shedding, Goat Diseases prevention & control, Goat Diseases microbiology, Goat Diseases immunology, Bacterial Vaccines immunology, Bacterial Vaccines administration & dosage, Vaccination methods, Vaccination veterinary, Antibodies, Bacterial blood, Antibodies, Bacterial immunology
- Abstract
A Coxiella burnetii vaccination program, targeting only doelings, was introduced on a German goat farm to curb bacterial shedding. In 2018, adults were vaccinated with a C. burnetii Phase I vaccine at three-weeks apart following pathogen diagnosis, with a booster administered six months later due to sustained high shedding. From 2018 to 2021, doelings received two vaccine doses without any further boosters. To assess the program's efficacy, vaginal swabs from up to 40 animals per age group were collected during kidding seasons from 2019 to 2022. Bulk tank milk (BTM) samples were gathered monthly from January 2018 to October 2022 to monitor herd-level shedding. Real-time PCR analysis determined genome equivalents in all three sample types. Serum samples were taken before the initial immunization and during the post-kidding season from up to 40 goats per age group annually from 2018 to 2022. Phase-specific ELISAs determined IgG Phase I and Phase II antibodies. Additionally, two serum samples per age group from 2022 were analyzed using a neutralization assay. A few goats continued shedding small quantities during subsequent kidding seasons. Although positive BTM samples decreased, they displayed an undulating trend. Most age groups exhibited robust IgG Phase I responses and lower IgG Phase II levels post immunization. Mean IgG levels remained elevated until the study ended compared to pre-vaccination levels in most age groups. Additionally, neutralizing antibodies were present regardless of IgG response. Overall, double vaccination induced lasting antibody levels, but did not entirely prevent C. burnetii shedding. The resilience of the observed humoral immune activity requires further investigation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
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- 2024
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36. Interdisciplinary studies on Coxiella burnetii: From molecular to cellular, to host, to one health research.
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Bauer BU, Knittler MR, Andrack J, Berens C, Campe A, Christiansen B, Fasemore AM, Fischer SF, Ganter M, Körner S, Makert GR, Matthiesen S, Mertens-Scholz K, Rinkel S, Runge M, Schulze-Luehrmann J, Ulbert S, Winter F, Frangoulidis D, and Lührmann A
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- Animals, Humans, Zoonoses epidemiology, Zoonoses prevention & control, Interdisciplinary Studies, Coxiella burnetii genetics, Q Fever epidemiology, Q Fever prevention & control, One Health
- Abstract
The Q-GAPS (Q fever GermAn interdisciplinary Program for reSearch) consortium was launched in 2017 as a German consortium of more than 20 scientists with exceptional expertise, competence, and substantial knowledge in the field of the Q fever pathogen Coxiella (C.) burnetii. C. burnetii exemplifies as a zoonotic pathogen the challenges of zoonotic disease control and prophylaxis in human, animal, and environmental settings in a One Health approach. An interdisciplinary approach to studying the pathogen is essential to address unresolved questions about the epidemiology, immunology, pathogenesis, surveillance, and control of C. burnetii. In more than five years, Q-GAPS has provided new insights into pathogenicity and interaction with host defense mechanisms. The consortium has also investigated vaccine efficacy and application in animal reservoirs and identified expanded phenotypic and genotypic characteristics of C. burnetii and their epidemiological significance. In addition, conceptual principles for controlling, surveilling, and preventing zoonotic Q fever infections were developed and prepared for specific target groups. All findings have been continuously integrated into a Web-based, interactive, freely accessible knowledge and information platform (www.q-gaps.de), which also contains Q fever guidelines to support public health institutions in controlling and preventing Q fever. In this review, we will summarize our results and show an example of how an interdisciplinary consortium provides knowledge and better tools to control a zoonotic pathogen at the national level., Competing Interests: Conflict of Interest The authors are members of the Zoonosis Consortium Q-GAPS (www.q-gaps.de) and declare no conflict of interest. The funders had no role in the study's design; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results., (Copyright © 2023 The Authors. Published by Elsevier GmbH.. All rights reserved.)
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- 2023
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37. [Innovative SARS-CoV-2 crisis management in the public health sector: Corona dashboard and wastewater surveillance using the example of Berchtesgadener Land, Germany].
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Roßmann K, Großmann G, Frangoulidis D, Clasen R, Münch M, Hasenknopf M, Wurzbacher C, Tiehm A, Stange C, Ho J, Woermann M, and Drewes JE
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- Germany epidemiology, Humans, Public Health, Wastewater, Wastewater-Based Epidemiological Monitoring, COVID-19 epidemiology, SARS-CoV-2
- Abstract
Background: The rise of an infectious disease crisis such as the SARS-CoV‑2 pandemic posed significant challenges for the administrative structures of the public health service, which resulted in varying levels of efficiency in outbreak management as a function of staffing and digital resources. This substantially impeded the integration of innovative pandemic outbreak management tools. Innovative crisis management, such as cluster tracking, risk group testing, georeferencing, or the integration of wastewater surveillance recommended by the EU Commission, was made significantly more difficult., Aim: In this case study in Berchtesgadener Land, we present the integration of an area-wide georeferenced wastewater surveillance system that captured 95% of the entire population since November 2020., Methodology: Sampling occurred twice a week at nine municipal wastewater treatment plants and directly from the main sewer at three locations. Samples were pre-treated by centrifugation and subsequently analyzed by digital droplet polymerase chain reaction (PCR) targeting four specific genes of SARS-CoV‑2., Results: The integration of an area-wide georeferenced wastewater surveillance system was successful. Wastewater occurrences are plotted for each municipality against cumulative infections over seven days per 100,000 inhabitants. Changes in the infection pattern in individual communities are noticeable ten days ahead of the official case numbers with a sensitivity of approximately 20 in 100,000 inhabitants., Discussion: The integration of this innovative approach to provide a comprehensive overview of the situation by employing a digital dashboard and the use of an early warning system via quantitative wastewater surveillance resulted in very efficient, proactive management, which might serve as a blueprint for other municipalities in Germany., (© 2021. The Author(s).)
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- 2022
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38. Small Mammals as Reservoir for Zoonotic Agents in Afghanistan.
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Essbauer S, Baumann K, Schlegel M, Faulde MK, Lewitzki J, Sauer SC, Frangoulidis D, Riehm JM, Dobler G, Teifke JP, Meyer H, and Ulrich RG
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- Afghanistan, Animals, Humans, Mammals microbiology, Mice, Rodentia, Rickettsia genetics
- Abstract
Introduction: Rodents and other small mammals can serve as reservoirs for a large number of zoonotic pathogens. A higher risk of infection with rodent-borne pathogens exists for humans with direct contact to rodents and/or their excretions, e.g., soldiers in operation areas. To date, little is known about endemic human pathogenic disease agents that are naturally associated with small mammals in Afghanistan. The aim of this study was to screen abundant rodents and insectivores collected from 2009 to 2012 in four field camps of the German Federal Armed Forces (Bundeswehr) in Northern Afghanistan for the presence of different pathogens., Materials and Methods: Isolated nucleic acids from ear pinna were screened by real-time PCR for spotted fever group (SFG) rickettsiae and from liver samples for Francisella spp., Coxiella burnetii, Brucella spp., Yersinia pestis, and poxvirus. Chest cavity lavage (CCL) samples were tested for antibodies against SFG and typhus group (TG) rickettsiae, as well as against flaviviruses using an indirect immunofluorescence assay., Results: Rickettsial DNA was detected in 7/750 (1%) ear pinna samples with one being identified as Rickettsia conorii. Antibodies against SFG rickettsiae were detected in 15.3% (n = 67/439) of the small mammals; positive samples were only from house mice (Mus musculus). Antibodies against TG rickettsiae were found in 8.2% (n = 36/439) of the samples, with 35 from house mice and one from gray dwarf hamster (Cricetulus migratorius). Flavivirus-reactive antibodies were detected in 2.3% (n = 10/439) of the investigated CCL samples; again positive samples were exclusively identified in house mice. All 199 investigated liver-derived DNA preparations were negative in the Francisella spp., C. burnetii, Brucella spp., Y. pestis, and poxvirus-specific PCRs., Conclusions: Further investigations will have to prove the potential value of rodents in army camps as sentinel animals., (© The Association of Military Surgeons of the United States 2021. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
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- 2022
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39. CoxBase: an Online Platform for Epidemiological Surveillance, Visualization, Analysis, and Typing of Coxiella burnetii Genomic Sequences.
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Fasemore AM, Helbich A, Walter MC, Dandekar T, Vergnaud G, Förstner KU, and Frangoulidis D
- Abstract
Q (query) fever is an infectious zoonotic disease caused by the Gram-negative bacterium Coxiella burnetii. Although the disease has been studied for decades, it still represents a threat due to sporadic outbreaks across farms in Europe. The absence of a central platform for Coxiella typing data management is an important epidemiological gap that is relevant in the case of an outbreak. To fill this gap, we have designed and implemented an online, open-source, web-based platform called CoxBase (https://coxbase.q-gaps.de). This platform includes a database that holds genotyping information on more than 400 Coxiella isolates alongside metadata that annotate them. We have also implemented features for in silico genotyping of completely or minimally assembled Coxiella sequences using five different typing methods, querying of existing isolates, visualization of isolate geodata via aggregation on a world map, and submission of new isolates. We tested our in silico typing method on 50 Coxiella genomes downloaded from the RefSeq database, and we successfully genotyped all genomes except for cases where the sequence quality was poor. We identified new spacer sequences using our implementation of the multispacer sequence typing (MST) in silico typing method and established adaA gene phenotypes for all 50 genomes as well as their plasmid types. IMPORTANCE Q fever is a zoonotic disease that is a source of active epidemiological concern due to its persistent threat to public health. In this project, we have identified areas in the field of Coxiella research, especially regarding public health and genomic analysis, where there is an inadequacy of resources to monitor, organize, and analyze genomic data from C. burnetii. Subsequently, we have created an open, web-based platform that contains epidemiological information, genome typing functions comprising all the available Coxiella typing methods, and tools for isolate data discovery and visualization that could help address the above-mentioned challenges. This is the first platform to combine all disparate genotyping systems for Coxiella burnetii as well as metadata assets with tools for genomic comparison and analyses. This platform is a valuable resource for laboratory researchers as well as research epidemiologists interested in investigating the relatedness or dissimilarity among C. burnetii strains.
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- 2021
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40. Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against Coxiella burnetii .
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Jeske R, Dangel L, Sauerbrey L, Frangoulidis D, Teras LR, Fischer SF, and Waterboer T
- Abstract
The causative agent of Q fever, the bacterium Coxiella burnetii ( C. burnetii ), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas. In order to conduct large-scale studies for population monitoring and to investigate possible associations more closely, accurate and cost-effective high-throughput assays are highly desired. To address this need, nine C. burnetii proteins were expressed as recombinant antigens for multiplex serology. This technique enables the quantitative high-throughput detection of antibodies to multiple antigens simultaneously in a single reaction. Based on a reference group of 76 seropositive and 91 seronegative sera, three antigens were able to detect C. burnetii infections. Com1, GroEL, and DnaK achieved specificities of 93%, 69%, and 77% and sensitivities of 64%, 72%, and 47%, respectively. Double positivity to Com1 and GroEL led to a combined specificity of 90% and a sensitivity of 71%. In a subgroup of seropositives with an increased risk for chronic Q fever, the double positivity to these markers reached a specificity of 90% and a sensitivity of 86%. Multiplex serology enables the detection of antibodies against C. burnetii and appears well-suited to investigate associations between C. burnetii infections and the clinical manifestations in large-scale studies.
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- 2021
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41. Multispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype of Coxiella burnetii .
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Bauer BU, Knittler MR, Herms TL, Frangoulidis D, Matthiesen S, Tappe D, Runge M, and Ganter M
- Abstract
A Q fever outbreak on a dairy goat and cattle farm was investigated with regard to the One Health concept. Serum samples and vaginal swabs from goats with different reproductive statuses were collected. Cows, cats, and a dog were investigated with the same sample matrix. The farmer's family was examined by serum samples. Ruminant sera were analyzed with two phase-specific enzyme-linked immunoassays (ELISAs). Dominant immunoglobulin G (IgG) phase II levels reflected current infections in goats. The cows had high IgG phase I and II levels indicating ongoing infections. Feline, canine, and human sera tested positive by indirect fluorescent antibody test (IFAT). Animal vaginal swabs were analyzed by qPCR to detect C. burnetii , and almost all tested positive. A new cattle-associated C. burnetii genotype C16 was identified by the Multiple-Locus Variable-number tandem repeat Analysis (MLVA/VNTR) from ruminant samples. Additionally, a possible influence of 17ß-estradiol on C. burnetii antibody response was evaluated in goat sera. Goats in early/mid-pregnancy had significantly lower levels of phase-specific IgGs and 17ß-estradiol than goats in late pregnancy. We conclude that the cattle herd may have transmitted C. burnetii to the pregnant goat herd, resulting in a Q fever outbreak with one acute human case. The influence of placentation and maternal pregnancy hormones during pregnancy on the immune response is discussed.
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- 2021
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42. COVID-19 Pandemic & Bureaucracy: The Crisis Inside the Crisis.
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Roßmann K, Wegner H, Stark H, Großmann G, Jansen A, and Frangoulidis D
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- Contact Tracing, Disease Outbreaks prevention & control, Humans, SARS-CoV-2, COVID-19, Pandemics
- Abstract
The Medical Intelligence and Information (MI2) Unit of the German Armed Forces (Bundeswehr) is experienced in crisis support in military missions since several years. It gained additional experiences during the current coronavirus 2019 (COVID-19) pandemic on different levels of the response to crisis and was requested to share the findings and expertise with the overloaded civil public health agencies inside Germany. Since the beginning of the pandemic, the unit is constantly developing new products for crisis communication, knowledge sharing techniques in new databases, dashboards for leadership, and training for laypersons in contact tracing. Hence, trying to innovate in crisis since the first severe acute respiratory syndrome coronavirus (SARS-CoV)-2-disease wave. During the second wave, the unit was requested to evaluate the outbreak management of different national civil public health agencies in southern Germany, and to support the development of dashboards in a comprehensive public health approach as a necessary start toward digitalization., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Roßmann, Wegner, Stark, Großmann, Jansen and Frangoulidis.)
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- 2021
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43. High Prevalence and New Genotype of Coxiella burnetii in Ticks Infesting Camels in Somalia.
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Frangoulidis D, Kahlhofer C, Said AS, Osman AY, Chitimia-Dobler L, and Shuaib YA
- Abstract
Coxiella burnetii is the causative agent of Q fever. It can infect animals, humans, and birds, as well as ticks, and it has a worldwide geographical distribution. To better understand the epidemiology of C. burnetii in Somalia, ticks infesting camels were collected from five different regions, including Bari, Nugaal, Mudug, Sool, and Sanaag, between January and March 2018. Collected ticks were tested for C. burnetii and Coxiella -like endosymbiont DNA by using IS1111, icd , and Com1 -target PCR assays. Moreover, sequencing of the 16S-rRNA was conducted. Molecular characterization and typing were done by adaA -gene analysis and plasmid-type identification. Further typing was carried out by 14-marker Multi-Locus Variable-Number Tandem Repeats (MLVA/VNTR) analysis. The investigated ticks ( n = 237) were identified as Hyalomma spp. ( n = 227, 95.8%), Amblyomma spp. ( n = 8, 3.4%), and Ripicephalus spp. ( n = 2, 0.8%), and 59.1% (140/237) of them were positive for Coxiella spp. While Sanger sequencing and plasmid-type identification revealed a C. burnetii that harbours the QpRS-plasmid, MLVA/VNTR genotyping showed a new genotype which was initially named D21. In conclusion, this is the first report of C. burnetii in ticks in Somalia. The findings denote the possibility that C. burnetii is endemic in Somalia. Further epidemiological studies investigating samples from humans, animals, and ticks within the context of "One Health" are warranted.
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- 2021
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44. Ticks (Acari: Ixodidae) on birds migrating to the island of Ponza, Italy, and the tick-borne pathogens they carry.
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Rollins RE, Schaper S, Kahlhofer C, Frangoulidis D, Strauß AFT, Cardinale M, Springer A, Strube C, Bakkes DK, Becker NS, and Chitimia-Dobler L
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- Animals, Bacteria isolation & purification, Bacterial Infections epidemiology, Bacterial Infections microbiology, Bacterial Infections veterinary, Bird Diseases microbiology, Bird Diseases parasitology, Incidence, Islands, Italy epidemiology, Ixodidae growth & development, Larva growth & development, Larva microbiology, Larva parasitology, Nymph growth & development, Nymph microbiology, Nymph parasitology, Piroplasmida isolation & purification, Prevalence, Protozoan Infections, Animal epidemiology, Protozoan Infections, Animal parasitology, Tick Infestations epidemiology, Tick Infestations parasitology, Tick-Borne Diseases epidemiology, Tick-Borne Diseases microbiology, Tick-Borne Diseases parasitology, Bird Diseases epidemiology, Ixodidae microbiology, Ixodidae parasitology, Songbirds, Tick Infestations veterinary, Tick-Borne Diseases veterinary
- Abstract
Seasonal migration of birds between breeding and wintering areas can facilitate the spread of tick species and tick-borne diseases. In this study, 151 birds representing 10 different bird species were captured on Ponza Island, an important migratory stopover off the western coast of Italy and screened for tick infestation. Ticks were collected and identified morphologically. Morphological identification was supported through sequencing a fragment of the 16S mitochondrial gene. In total, 16 captured birds carried ticks from four tick species: Hyalomma rufipes (n = 14), Amblyomma variegatum (n = 1), Amblyomma sp. (n = 1), and Ixodes ventalloi (n = 2). All specimens were either larvae (n = 2) or nymphs (n = 16). All ticks were investigated for tick-borne pathogens using published molecular methods. Rickettsia aeschlimannii was detected in six of the 14 collected H. rufipes ticks. Additionally, the singular A. variegatum nymph tested positive for R. africae. In all 14 H. rufipes specimens (2 larvae and 12 nymphs), Francisella-like endosymbionts were detected. Four H. rufipes ticks tested positive for Borrelia burgdorferi sensu lato in a screening PCR but did not produce sufficient amplicon amounts for species identification. All ticks tested negative for tick-borne encephalitis virus, Crimean-Congo hemorrhagic fever virus, Coxiella burnetii, Coxiella-like organisms, Babesia spp., and Theileria spp. This study confirms the role of migratory birds in the spread and establishment of both exotic tick species and tick-borne pathogens outside their endemic range., (Copyright © 2020 Elsevier GmbH. All rights reserved.)
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- 2021
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45. Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype.
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Bauer B, Prüfer L, Walter M, Ganter I, Frangoulidis D, Runge M, and Ganter M
- Abstract
The main reservoir of Coxiella (C.) burnetii are ruminants. They shed the pathogen through birth products, vaginal mucus, faeces and milk. A direct comparison of C. burnetii excretions between naturally infected sheep and goats was performed on the same farm to investigate species-specific differences. The animals were vaccinated with an inactivated C. burnetii phase I vaccine at the beginning of the study period for public health reasons. Vaginal and rectal swabs along with milk specimens were taken monthly during the lambing period and once again at the next lambing season. To estimate the environmental contamination of the animals' housings, nasal swabs from every animal were taken simultaneously. Moreover, dust samples from the windowsills and straw beddings were collected. All samples were examined by qPCR targeting the IS 1111 gene and the MLVA/VNTR typing method was performed. Whole genome sequencing was applied to determine the number of IS 1111 copies followed by a calculation of C. burnetii genome equivalents of each sample. The cattle-associated genotype C7 was detected containing 29 IS 1111 copies. Overall, goats seem to shed more C. burnetii through vaginal mucus and in particular shed more and for longer via the rectal route than sheep. This is supported by the larger quantities of C. burnetii DNA detected in caprine nasal swabs and environmental samples compared to the ovine ones. Transmission of C. burnetii from cattle to small ruminants must also be considered.
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- 2020
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46. Current perspectives on the transmission of Q fever: Highlighting the need for a systematic molecular approach for a neglected disease in Africa.
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Salifu SP, Bukari AA, Frangoulidis D, and Wheelhouse N
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- Africa epidemiology, Animals, Humans, Livestock, Molecular Epidemiology, Neglected Diseases epidemiology, Prevalence, Risk Factors, Q Fever epidemiology, Q Fever transmission
- Abstract
Q fever is a bacterial worldwide zoonosis (except New Zealand) caused by the Gram-negative obligate intracellular bacterium Coxiella burnetii (C. burnetii). The bacterium has a large host range including arthropods, wildlife and companion animals and is frequently identified in human and livestock populations. In humans, the disease can occur as either a clinically acute or chronic aetiology, affecting mainly the lungs and liver in the acute disease, and heart valves when chronic. In livestock, Q fever is mainly asymptomatic; however, the infection can cause abortion, and the organism is shed in large quantities, where it can infect other livestock and humans. The presence of Q fever in Africa has been known for over 60 years, however while our knowledge of the transmission routes and risk of disease have been well established in many parts of the world, there is a significant paucity of knowledge across the African continent, where it remains a neglected zoonosis. Our limited knowledge of the disease across the African sub-continent have relied largely upon observational (sero) prevalence studies with limited focus on the molecular epidemiology of the disease. This review highlights the need for systematic studies to understand the routes of C. burnetii infection, and understand the disease burden and risk factors for clinical Q fever in both humans and livestock. With such knowledge gaps filled, the African continent could stand a better chance of eradicating Q fever through formulation and implementation of effective public health interventions., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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47. Imported Hyalomma ticks in Germany in 2018.
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Chitimia-Dobler L, Schaper S, Rieß R, Bitterwolf K, Frangoulidis D, Bestehorn M, Springer A, Oehme R, Drehmann M, Lindau A, Mackenstedt U, Strube C, and Dobler G
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- Animals, Female, Germany, Horses parasitology, Humans, Male, Rickettsia classification, Rickettsia isolation & purification, Sheep parasitology, Ixodidae microbiology, Ixodidae parasitology
- Abstract
Background: Hyalomma marginatum and Hyalomma rufipes are two-host tick species, which are mainly distributed in southern Europe, Africa and middle-eastern Asia. They are well-known vectors of Crimean Congo hemorrhagic fever (CCHF) virus and other viruses as well as Rickettsia aeschlimannii. In recent years, these tick species have been found sporadically in Germany, but they do not belong to the autochthonous tick fauna in Germany., Methods: Ticks with unusual morphology were collected and sent from private persons or public health offices to involve institutions for morphological identification and further testing. All ticks identified as Hyalomma spp. were tested using molecular detection methods for CCHF virus, Rickettsia spp., Coxiella burnetii and Coxiella-like organisms, Babesia spp. and Theileria spp., Results: Thirty-five ticks with an unusual appearance or behaviour were reported to us during summer-autumn 2018. For 17 of them, the description or photos implied that they belong to the hard tick genus Hyalomma. The remaining 18 ticks were sent to us and were identified as adult Hyalomma marginatum (10 specimens) or adult Hyalomma rufipes (8 specimens). All ticks tested negative for CCHF virus, Coxiella burnetii, Coxiella-like organisms, Babesia spp. and Theileria spp. The screening for rickettsiae gave positive results in 9 specimens . The Rickettsia species in all cases was identified as R. aeschlimannii., Conclusions: These results show that exotic tick species imported into Germany were able to develop from the nymphal to the adult stage under appropriate weather conditions. Fifty percent of the ticks carried R. aeschlimannii, a human pathogen, while CCHF virus or other pathogens were not detected. Imported Hyalomma ticks may be the source of exotic diseases acquired in Germany.
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- 2019
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48. New records and host associations of the tick Ixodes apronophorus and the first detection of Ehrlichia sp. HF in Romania.
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Andersson MO, Radbea G, Frangoulidis D, Tomaso H, Rubel F, Nava S, and Chitimia-Dobler L
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- Animals, Arachnid Vectors classification, Arachnid Vectors genetics, DNA, Ribosomal genetics, Ehrlichia classification, Ehrlichia genetics, Europe, Eastern, Female, Geography, Ixodes genetics, RNA, Ribosomal genetics, RNA, Ribosomal, 16S genetics, Rabbits, Romania epidemiology, Arachnid Vectors microbiology, Dogs microbiology, Ehrlichia isolation & purification, Foxes microbiology, Ixodes classification
- Abstract
Ixodes (Ixodes) apronophorus is a neglected tick species and its geographical distribution, host associations, and role as a disease vector are not well known. We collected I. apronophorus from several locations in Romania. Morphological identification of ticks was confirmed by analysis of 16S rDNA and 12S rDNA gene sequences. We report new host associations of I. apronophorus, which was collected from dogs, foxes, and a hare-all new hosts for this tick species in Romania. Furthermore, we report for the first time occurrence of Ehrlichia sp. HF in I. apronophorus. Ehrlichia sp. HF was identified by sequencing a part of the 16S rDNA gene and was found in 16% (3/19) of the tested ticks. Ehrlichia sp. HF has not been previously reported in Eastern Europe and seems to have a much larger geographic distribution than previously known. Currently, it is unknown whether I. apronophorus is a competent vector for Ehrlichia sp. HF, or if the findings in this study represent infection in the hosts, namely dogs and fox.
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- 2018
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49. Molecular survey of neglected bacterial pathogens reveals an abundant diversity of species and genotypes in ticks collected from animal hosts across Romania.
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Andersson MO, Tolf C, Tamba P, Stefanache M, Radbea G, Frangoulidis D, Tomaso H, Waldenström J, Dobler G, and Chitimia-Dobler L
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- Anaplasmataceae genetics, Anaplasmataceae isolation & purification, Anaplasmataceae pathogenicity, Animals, Bacteria isolation & purification, Bacteria pathogenicity, Dogs, Ehrlichia genetics, Ehrlichia isolation & purification, Ehrlichia pathogenicity, Neglected Diseases epidemiology, Neglected Diseases microbiology, Rickettsia genetics, Rickettsia isolation & purification, Rickettsia pathogenicity, Romania epidemiology, Sheep, Tick Infestations veterinary, Zoonoses epidemiology, Zoonoses microbiology, Bacteria genetics, Genotype, Ixodidae microbiology, Neglected Diseases veterinary
- Abstract
Background: Ticks are transmitting a wide range of bacterial pathogens that cause substantial morbidity and mortality in domestic animals. The full pathogen burden transmitted by tick vectors is incompletely studied in many geographical areas, and extensive studies are required to fully understand the diversity and distribution of pathogens transmitted by ticks., Results: We sampled 824 ticks of 11 species collected in 19 counties in Romania. Ticks were collected mainly from dogs, but also from other domestic and wild animals, and were subjected to molecular screening for pathogens. Rickettsia spp. was the most commonly detected pathogen, occurring in 10.6% (87/824) of ticks. Several species were detected: Rickettsia helvetica, R. raoultii, R. massiliae, R. monacensis, R. slovaca and R. aeschlimannii. A single occurrence of the zoonotic bacterium Bartonella vinsonii berkhoffii was detected in a tick collected from a dog. Anaplasma phagocytophilum occurred in four samples, and sequences similar to Anaplasma marginale/ovis were abundant in ticks from ruminants. In addition, molecular screening showed that ticks from dogs were carrying an Ehrlichia species identical to the HF strain as well as the enigmatic zoonotic pathogen "Candidatus Neoehrlichia mikurensis". An organism similar to E. chaffeensis or E. muris was detected in an Ixodes ricinus collected from a fox., Conclusions: We describe an abundant diversity of bacterial tick-borne pathogens in ticks collected from animal hosts in Romania, both on the level of species and genotypes/strains within these species. Several findings were novel for Romania, including Bartonella vinsonii subsp. berkhoffii that causes bacteremia and endocarditis in dogs. "Candidatus Neoehrlichia mikurensis" was detected in a tick collected from a dog. Previously, a single case of infection in a dog was diagnosed in Germany. The results warrant further studies on the consequences of tick-borne pathogens in domestic animals in Romania.
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- 2018
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50. The Intervening Sequence of Coxiella burnetii: Characterization and Evolution.
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Warrier I, Walter MC, Frangoulidis D, Raghavan R, Hicks LD, and Minnick MF
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- Amino Acid Sequence, Base Sequence, Coxiella burnetii growth & development, Coxiella burnetii metabolism, DNA, Bacterial genetics, DNA, Ribosomal genetics, Escherichia coli genetics, Evolution, Molecular, Gene Transfer, Horizontal, Genes, Bacterial, Nucleic Acid Conformation, Phylogeny, Protein Structure, Secondary, Q Fever microbiology, RNA Splicing, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Ribonuclease III genetics, Coxiella burnetii genetics, Introns, RNA, Ribosomal, 23S genetics
- Abstract
The intervening sequence (IVS) of Coxiella burnetii, the agent of Q fever, is a 428-nt selfish genetic element located in helix 45 of the precursor 23S rRNA. The IVS element, in turn, contains an ORF that encodes a hypothetical ribosomal S23 protein (S23p). Although S23p can be synthesized in vitro in the presence of an engineered E. coli promoter and ribosome binding site, results suggest that the protein is not synthesized in vivo. In spite of a high degree of IVS conservation among different strains of C. burnetii, the region immediately upstream of the S23p start codon is prone to change, and the S23p-encoding ORF is evidently undergoing reductive evolution. We determined that IVS excision from 23S rRNA was mediated by RNase III, and IVS RNA was rapidly degraded, thereafter. Levels of the resulting 23S rRNA fragments that flank the IVS, F1 (~1.2 kb) and F2 (~1.7 kb), were quantified over C. burnetii's logarithmic growth phase (1-5 d). Results showed that 23S F1 quantities were consistently higher than those of F2 and 16S rRNA. The disparity between levels of the two 23S rRNA fragments following excision of IVS is an interesting phenomenon of unknown significance. Based upon phylogenetic analyses, IVS was acquired through horizontal transfer after C. burnetii's divergence from an ancestral bacterium and has been subsequently maintained by vertical transfer. The widespread occurrence, maintenance and conservation of the IVS in C. burnetii imply that it plays an adaptive role or has a neutral effect on fitness.
- Published
- 2016
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