34 results on '"Francesca Spanò"'
Search Results
2. Autoimmune central diabetes insipidus in a patient with ureaplasma urealyticum infection and review on new triggers of immune response
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Giuseppe Murdaca, Rodolfo Russo, Francesca Spanò, Diego Ferone, Manuela Albertelli, Angelo Schenone, Miriam Contatore, Andrea Guastalla, Annamaria De Bellis, Giacomo Garibotto, and Francesco Puppo
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Medicine ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Diabetes insipidus is a disease in which large volumes of dilute urine (polyuria) are excreted due to vasopressin (AVP) deficiency [central diabetes insipidus (CDI)] or to AVP resistance (nephrogenic diabetes insipidus). In the majority of patients, the occurrence of CDI is related to the destruction or degeneration of neurons of the hypothalamic supraoptic and paraventricular nuclei. The most common and well recognized causes include local inflammatory or autoimmune diseases, vascular disorders, Langerhans cell histiocytosis (LCH), sarcoidosis, tumors such as germinoma/craniopharyngioma or metastases, traumatic brain injuries, intracranial surgery, and midline cerebral and cranial malformations. Here we have the opportunity to describe an unusual case of female patient who developed autoimmune CDI following ureaplasma urealyticum infection and to review the literature on this uncommon feature. Moreover, we also discussed the potential mechanisms by which ureaplasma urealyticum might favor the development of autoimmune CDI.
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- 2015
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3. Primary mediastinal large B-cell lymphoma and pregnancy: a challenging clinical scenario
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Rita, Intravaia, Benedetta, De Chiara, Francesco, Musca, Francesca, Casadei, Gloria, Santambrogio, Francesca, Spanò, Oriana, Belli, Giuseppina, Quattrocchi, Cristina, Giannattasio, Antonella, Moreo, Intravaia, R, De Chiara, B, Musca, F, Casadei, F, Santambrogio, G, Spanò, F, Belli, O, Quattrocchi, G, Giannattasio, C, and Moreo, A
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Dyspnea ,Lymphoma ,Mediastinum ,Humans ,imaging ,Female ,Lymphoma, Large B-Cell, Diffuse ,pregnancy ,chemotherapy ,Mediastinal Neoplasms - Abstract
A 26-weeks pregnant woman presented with progressively worsening dyspnoea and poor general conditions. Using low-dose radiation multi-imaging techniques and thoracic biopsy a primary mediastinal large B cell was diagnosed. A multidisciplinary approach identified the correct hemodynamic management, the best therapeutic strategy and the timing for delivery.
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- 2022
4. Prevalence of hypertension mediated organ damage in subjects with high-normal blood pressure without known hypertension as well as cardiovascular and kidney disease
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Lucia Occhi, Francesca Spanò, Francesca Casadei, G Santambrogio, M. Alloni, Francesco Musca, Alessandro Maloberti, Antonella Moreo, Giuseppe Occhino, Cristina Giannattasio, Oriana Belli, Paola Rebora, Maria Grazia Valsecchi, Benedetta De Chiara, Maloberti, A, Rebora, P, Occhino, G, Alloni, M, Musca, F, Belli, O, Spano, F, Santambrogio, G, Occhi, L, De Chiara, B, Casadei, F, Moreo, A, Valsecchi, M, and Giannattasio, C
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medicine.medical_specialty ,Blood Pressure ,Pulse Wave Analysis ,Left ventricular hypertrophy ,Carotid Intima-Media Thickness ,Internal medicine ,Internal Medicine ,medicine ,Prevalence ,Humans ,cardiovascular diseases ,Pulse wave velocity ,business.industry ,Healthy population ,medicine.disease ,arterial hypertenson, organ damage ,Normal group ,Organ damage ,Blood pressure ,Carotid Arteries ,Hypertension ,cardiovascular system ,Arterial stiffness ,Cardiology ,Kidney Diseases ,business ,Kidney disease - Abstract
Purpose of our study was to assess the prevalence of hypertension mediated organ damage (HMOD) in healthy subjects with high-normal Blood Pressure (BP) comparing them with subjects with BP values that are considered normal (
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- 2021
5. Could two-dimensional radial strain be considered as a novel tool to identify pre-clinical hypertrophic cardiomyopathy mutation carriers?
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Francesca Casadei, Rita Facchetti, Cristina Giannattasio, Emanuela Manfredini, Francesco Musca, Alessandro Maloberti, Francesca Spanò, Lucia Occhi, Fabio Turazza, Oriana Belli, G Santambrogio, Antonella Moreo, P. Vallerio, Angelica Peritore, Benedetta De Chiara, Santambrogio, G, Maloberti, A, Vallerio, P, Peritore, A, Spanò, F, Occhi, L, Musca, F, Belli, O, De Chiara, B, Casadei, F, Facchetti, R, Turazza, F, Manfredini, E, Giannattasio, C, and Moreo, A
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,TNNT2 ,DNA Mutational Analysis ,030204 cardiovascular system & hematology ,Left ventricular hypertrophy ,Ventricular Function, Left ,Strain ,Muscle hypertrophy ,Young Adult ,03 medical and health sciences ,Basal (phylogenetics) ,0302 clinical medicine ,Troponin T ,Predictive Value of Tests ,Internal medicine ,medicine ,Humans ,Genetic Predisposition to Disease ,Radiology, Nuclear Medicine and imaging ,030212 general & internal medicine ,Aged ,Myosin Heavy Chains ,Receiver operating characteristic ,business.industry ,Hypertrophic cardiomyopathy ,Reproducibility of Results ,Cardiomyopathy, Hypertrophic ,Middle Aged ,medicine.disease ,Phenotype ,Echocardiography ,Case-Control Studies ,Mutation ,Mutation (genetic algorithm) ,Sarcomeric mutation ,Cardiology ,Female ,MYH7 ,Carrier Proteins ,Cardiology and Cardiovascular Medicine ,business ,Cardiac Myosins - Abstract
Treatment of overt form of hypertrophic cardiomyopathy (HCM) is often unsuccessful. Efforts are focused on a possible early identification in order to prevent or delaying the development of hypertrophy. Our aim was to find an echocardiographic marker able to distinguish mutation carriers without left ventricular hypertrophy (LVH) from healthy subjects. We evaluated 28 patients, members of eight families. Three types of mutation were recognized: MYBPC3 (five families), MYH7 (two families) and TNNT2 (one family). According to genetic (G) and phenotypic (Ph) features, patients were divided in three groups: Group A (10 patients), mutation carriers with LVH (G+/Ph+); Group B (9 patients), mutation carriers without LVH (G+/Ph−); Group C (9 patients), healthy subjects (G−/Ph−). Echocardiography examination was performed acquiring standard 2D, DTI and 2D-strain imaging. Global longitudinal strain (GLS) and global radial strain (GRS) at basal and mid-level were measured. GRS was significantly different between group B and C at basal level (32.18% ± 9.6 vs. 44.59% ± 12.67 respectively; p-value < 0.0001). In basal posterior and basal inferior segments this difference was particularly evident. ROC curves showed for both the involved segments good AUCs (0.931 and 0.861 for basal posterior and inferior GRS respectively) with the best predictive cut-off for basal posterior GRS at 43.65%, while it was 38.4% for basal inferior GRS. Conversely, GLS values were similar in the three group. 2D longitudinal strain is a valid technique to study HCM. Radial strain and particularly basal posterior and inferior segmental reduction could be able to identify mutation carriers in a pre-clinical phase of disease.
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- 2019
6. Hemodialysis: effects of preload reduction on novel echocardiographic parameters of left ventricular function
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Oriana Belli, C Brunati, Francesca Casadei, Francesca Spanò, B. De Chiara, Francesco Musca, F Gervasi, Giovanni Tonti, Antonella Moreo, Cristina Giannattasio, Martina Milani, G Santambrogio, and L. F. Cerrito
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medicine.medical_specialty ,Ventricular function ,business.industry ,medicine.medical_treatment ,General Medicine ,Preload ,Internal medicine ,medicine ,Cardiology ,Radiology, Nuclear Medicine and imaging ,Hemodialysis ,Cardiology and Cardiovascular Medicine ,business ,Reduction (orthopedic surgery) - Abstract
Funding Acknowledgements Type of funding sources: None. Background Echocardiography has been widely used to study cardiac function in patients with end-stage renal disease on hemodialysis (HD), but cardiac function assessment by measuring cardiac dimensions and their rate of change is load dependent, therefore it is influenced by volume depletion. Effects of acute volume reduction on left (LV) and right ventricular (RV) function are still not well understood. Some studies investigated myocardial mechanics after dialysis using speckle tracking echocardiography (STE) but their relative load-dependency makes STE indices unable to account for changes in pre- and afterload. Myocardial work (MW) incorporates both deformation and load into its analysis and is an emerging tool to study LV myocardial function. There are no data about the effects of hemodialysis on LV MW. Purpose This study aimed to evaluate acute changes of novel echocardiographic indices of both LV and RV function after a HD session. Methods Patients with end-stage renal disease undergoing HD were prospectively enrolled. A transthoracic echo, including STE calculation of LV global longitudinal strain (GLS) and free wall RV strain, was performed before and after hemodialysis. Parameters of MW such as global work index (GWI), global constructive work (GCW), global work efficiency (GWE) and global wasted work (GWW) were quantified using a commercially available software package. Results 27 patients were enrolled, mean baseline parameters were: LV end-diastolic volume 136 ± 38 mL, LV ejection fraction (LVEF) 56.9 ± 7.5%, LV GLS -17.1 ± 4.1%, RV free wall strain -26.9 ± 5.6%, GWI 2117 ± 602 mmHg%, GCW 2299 ± 633 mmHg%, GWW 137 ± 88 mmHg, GWE 93 ± 3.6%, systolic arterial pressure 145 ± 26 mmHg and diastolic pressure 80 ± 16mmHg. After hemodialysis we observed a significative reduction in LV GLS (p = 0.04), RV strain (p = 0.002), GWI (p = 0.002, Figure I) and GCW (p = 0.004). No significative changes in LVEF and blood pressure were observed. Comparing patients using a LVEF cut-off of 55% (19 patients with LVEF≥55%, 8 patients Conclusions Our preliminary data show that, immediately after the HD session, there is a reduction in biventricular STE-derived systolic parameters. Patients with normal LV systolic function are more sensitive to acute volume changes and entity of volume depletion seems to be correlated with MW reduction. Abstract Figure.
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- 2021
7. Functional improvement after cardiac rehabilitation is not related to improvement in left ventricular ejection fraction
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A. Peretti, S. Sioli, Cristina Giannattasio, D. Caroti, S. Bordoni, G. Beretta, Francesca Casadei, G Santambrogio, B. De Chiara, Antonella Moreo, Francesco Musca, F. Esposito, N. Triglione, A.M. Pane, Oriana Belli, S. Riccobono, Francesca Spanò, L. Amoruso, Alessandro Maloberti, Laura Garatti, Maloberti, A, Peretti, A, Garatti, L, Triglione, N, Sioli, S, Bordoni, S, Amoruso, L, Caroti, D, Pane, A, Musca, F, Belli, O, De Chiara, B, Casadei, F, Sant’Ambrogio, G, Spanò, F, Esposito, F, Moreo, A, Beretta, G, Riccobono, S, Giannattasio, C, and Spano’, F
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medicine.medical_specialty ,Ejection fraction ,Rehabilitation ,Physiology ,business.industry ,medicine.medical_treatment ,Internal medicine ,functional improvement, cardiac rehabilitation, left ventricular ejection fraction ,Internal Medicine ,medicine ,Cardiology ,cardiac rehabilitation, improvement, left ventricular ejection fraction ,Cardiology and Cardiovascular Medicine ,business - Abstract
Introduction: Cardiac rehabilitation (CR) improves the functional capacity and the prognosis of patients with coronary artery disease (CAD). Similar results have also been found in patients with dilated cardiomyopathy (DCM). Aim: To assess the relationship between functional improvement (evaluated with 6-minute walking test–6MWT) and the improvement in left ventricular ejection fraction (LVEF) after CR. Methods: we collected data from 260 patients that performed CR after an Acute Coronary Syndrome (ACS). The functional improvement after CR was expressed as the Δ between distance covered at the final versus the initial 6MWT normalized for the initial 6MWT, while LVEF was calculated with transthoracic echocardiogram at the beginning and at the end of the CR. Results: in the whole population functional improvement was 44.07% (baseline 6MWT 421.22 m vs follow-up 6MWT 597.28 m, p ≤ 0.05) while EF improvement was 2.48% (baseline EF 53.37% vs follow-up EF 55.91%, p ≤ 0.05). No significant correlation between the normalized Δmeter and ΔEF was founded. When patients were divided accordingly to their pre-rehab LVEF (≥ 55, 40–55 and
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- 2019
8. CLINICAL AND ECHOCARDIOGRAPHIC FEATURES OF PATIENTS WITH HEART FAILURE WITH MID-RANGE EJECTION FRACTION COMPARED TO REDUCED AND PRESERVED
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Sofia Bianchi, Alessandro Maloberti, Paola Sormani, Claudia Pellegrinelli, Valentina Giani, Umberto Ceratti, Francesco Musca, Francesca Spanò, Cristina Giannattasio, Marco Biolcati, Francesca Casadei, Benedetta De Chiara, Oriana Belli, G Santambrogio, and Antonella Moreo
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medicine.medical_specialty ,Ejection fraction ,Physiology ,business.industry ,Heart failure ,Internal medicine ,Internal Medicine ,medicine ,Cardiology ,Cardiology and Cardiovascular Medicine ,medicine.disease ,business - Published
- 2021
9. Adapted Training to Boost Upper Body Sensorimotor Control and Daily Living Functionality in Visually Impaired Baseball Players
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Giuditta Carretti, Francesca Spano, Eleonora Sgambati, Mirko Manetti, and Mirca Marini
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visual disability ,adapted sport ,blind baseball ,sensorimotor training ,daily living autonomy ,proprioception ,Medicine (General) ,R5-920 - Abstract
Background and Objectives: Vision significantly contributes to postural control, balance, coordination, and body kinematics, thus deeply influencing everyday functionality. Sight-impaired subjects often show upper body anatomofunctional and kinetic chain alterations negatively impacting daily living efficiency and autonomy. The present study aimed to investigate and train, for the first time, upper body sensorimotor control in an Italian blind baseball team to boost global and segmental functionality while contemporarily prevent injuries. Materials and Methods: The whole team underwent a validated test battery using both quantitative traditional tools, such as goniometric active range of motion and muscular/functional tests, and an innovative biofeedback-based device, a Libra proprioceptive board. Consequently, a 6-week adapted training protocol was designed and leaded to improve sensorimotor control and, hence, counteract disability-related deficits and sport-specific overuse syndromes. Results: Statistically significant improvements were observed in all the investigated parameters. Noteworthy, an overall boost of global and segmental stability was detected through an orthostatic dynamic balance enhancement during the Y Balance test (p = 0.01) and trunk multiplanar control improvement on the Libra board (p = 0.01). Concurrently, the comparison of baseline vs. post-intervention outcomes revealed a consistent increase in upper body mobility (p < 0.05 for all the assessed districts), core recruitment (p = 0.01 for all the administered functional tests), and proprioceptive postural control (p = 0.01 for the Libra board validated test). Conclusions: Our findings suggest that a tailored sensorimotor training, conceived and led by an adapted physical activity kinesiologist, may effectively improve upper body functional prerequisites and global proprioceptive control, thus potentially promoting autonomy, quality of life, and physical activity/sport practice adherence in visually impaired individuals.
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- 2024
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10. Vaccine-preventable infections in Systemic Lupus Erythematosus
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Giancarlo Icardi, Andrea Orsi, Giuseppe Murdaca, Filippo Ansaldi, Paolo Durando, V Faccio, Francesco Puppo, and Francesca Spanò
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systemic lupus ,Immunology ,Reviews ,03 medical and health sciences ,0302 clinical medicine ,immune system diseases ,vaccine-preventable ,Humans ,Lupus Erythematosus, Systemic ,Immunology and Allergy ,Medicine ,infections ,030212 general & internal medicine ,skin and connective tissue diseases ,030203 arthritis & rheumatology ,Pharmacology ,Vaccines ,Systemic lupus erythematosus ,business.industry ,Systemic lupus ,Disease expression ,Immunogenicity ,Risk of infection ,erythematosus ,vaccines ,Bacterial Infections ,medicine.disease ,Vaccination ,Virus Diseases ,business ,Autoantibody production - Abstract
Systemic Lupus Erythematosus (SLE) is characterized by abnormal autoantibody production and clearance. Infections are among the most important causes of morbidity and mortality in SLE patients; they have an increased frequency of severe bacterial and viral infections possibly due to inherited genetic and immunologic defects and to immunosuppressive therapies. In addition, infectious agents can switch on lupus disease expression and activity. Among the strategies to reduce the risk of infection, vaccination can be considered the most reliable option. Most vaccines are effective and safe in SLE patients, although in certain cases immunogenicity may be sub-optimal and vaccination can trigger a flare. Although these issues are currently unresolved, the risk benefit balance is in favor for vaccination to reduce the risk of infection in SLE patients. In the present review we discuss the preventive strategies currently recommended to reduce bacterial and viral infections in SLE.
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- 2016
11. Infection risk associated with anti-TNF-α agents: a review
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Elena Penza, Giuseppe Murdaca, Ottavia Magnani, Andrea Guastalla, Miriam Contatore, Francesca Spanò, and Francesco Puppo
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Hepatitis B virus ,Tuberculosis ,medicine.medical_treatment ,Infections ,Hepatitis C virus ,TNF-α inhibitors ,Antirheumatic Agents ,Etanercept ,Humans ,Immune System Diseases ,Immunologic Factors ,Infection ,Infliximab ,Risk Factors ,Tumor Necrosis Factor-alpha ,Pharmacology (medical) ,Medicine (all) ,Pathogenesis ,medicine ,Medical history ,medicine.diagnostic_test ,Latent tuberculosis ,business.industry ,General Medicine ,Mantoux test ,medicine.disease ,Cytokine ,Immunology ,business ,medicine.drug - Abstract
TNF-α is a pro-inflammatory cytokine known to a have a key role in the pathogenesis of chronic immune-mediated diseases. TNF-α inhibitors can be administered either as monotherapy or in combination with other anti-inflammatory or disease-modifying anti-rheumatic drugs (DMARDs) to treat chronic immune-mediated diseases.Patients receiving TNF-α inhibitors are at high risk of infections. Based on our experience, in this paper, we discuss the risk of infections associated with the administration of TNF-α inhibitors and the strategies for mitigating against the development of these serious adverse events.Infliximab more so than etanercept appears to be responsible for the increased risk of infections. Re-activation of latent tuberculosis (LTB) infection and the overall risk of opportunistic infections should be considered before beginning TNF-α inhibitor therapy. A careful medical history, Mantoux test and chest-x-ray should always be performed before prescribing TNF-α inhibitors. Particular attention should be paid to risk factors for Pneumocystis jirovecii infection. Hepatitis B and C virological follow-up should be considered during TNF-α inhibitor treatment. Finally, patients who are at high risk of herpes zoster (HZ) reactivation would benefit from a second vaccination in adulthood when receiving TNF-α inhibitors.
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- 2015
12. Pharmacogenetics and Future Therapeutic Scenarios: What Affects the Prediction of Response to Treatment with Etanercept?
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Rossella Gulli, Paola Mandich, Francesca Spanò, Giuseppe Murdaca, and Francesco Puppo
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business.industry ,Single-nucleotide polymorphism ,Genomics ,Bioinformatics ,Infliximab ,Golimumab ,Etanercept ,Drug Discovery ,Immunology ,Adalimumab ,medicine ,Certolizumab pegol ,business ,Pharmacogenetics ,medicine.drug - Abstract
Enabling Technology, Genomics, Proteomics Clinical Development Phases I-III Regulatory, Quality, Manufacturing There are five tumor necrosis factor alpha (TNF-α) inhibitors available for clinical use that have demonstrated efficacy as monotherapy or in combination with other anti-inflammatory or disease-modifying anti-rheumatic drugs (DMARDs) in the treatment of immune-mediated diseases. These include the anti-TNF-α monoclonal antibodies infliximab, adalimumab, golimumab, and certolizumab pegol, and the fusion protein, etanercept. The use of pharmacogenetic testing has the potential to increase drug efficiency by identifying genetic factors responsible for a lack of response to, or toxicities from, TNF-α inhibitors, and could be used to individualize therapy. Several studies have reported associations between genetic polymorphisms and the response to etanercept, but most are small and insufficiently powered to detect effect, and markers tend to be more prognostic than predictive of therapeutic response. Limitations of pharmacogenetic studies include the use of single nucleotide polymorphisms (SNPs), genes in linkage with other loci, interaction of environmental factors, and cohort heterogeneity, all of which can complicate the relationship between genetic polymorphisms and treatment response. Further studies are needed for pharmacogenetics to become a routine part of daily clinical therapeutic practice.
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- 2014
13. Influenza and pneumococcal vaccinations of patients with systemic lupus erythematosus: Current views upon safety and immunogenicity
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Giancarlo Icardi, Paolo Durando, Andrea Orsi, Giuseppe Murdaca, Francesca Spanò, Francesco Puppo, and Filippo Ansaldi
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education.field_of_study ,Exacerbation ,business.industry ,Influenza vaccine ,Immunogenicity ,Immunology ,Population ,Autoantibody ,Disease ,Pneumococcal Vaccines ,Vaccination ,Immunocompromised Host ,Pneumococcal vaccine ,Influenza Vaccines ,Influenza, Human ,Practice Guidelines as Topic ,Humans ,Lupus Erythematosus, Systemic ,Immunology and Allergy ,Medicine ,business ,education - Abstract
Systemic lupus erythematosus (SLE) is a chronic immune-mediated inflammatory multisystem disease. The onset of viral and bacterial infections may favor the exacerbation of the disease, amplify autoimmune processes and contribute to mortality and morbidity. The prevention of influenza and Streptococcus pneumoniae infections with vaccination should receive particular attention in SLE patients considering their elevated incidence, their high attack rate in epidemic periods, their potentially severe complications as well as the immunocompromised state of the host. The use of non-adjuvanted vaccine preparations should be preferred in order to avoid the onset of the "Autoimmune (auto-inflammatory) Syndrome Induced by Adjuvants" or "ASIA". In this review, we report that influenza and pneumococcal vaccinations in SLE patients are: 1) recommended to reduce the risk of development of these infections; 2) strongly suggested in elderly subjects and in those receiving high dose immunosuppressive treatments; 3) efficacious, even if specific immune responses may be lower than in the general population, as generally the humoral response fulfills the criteria for vaccine immunogenicity; and 4) safe in inactive disease although may favor a transient increase in autoantibody levels and rarely disease flares.
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- 2014
14. Current therapies for the treatment of systemic sclerosis-related pulmonary arterial hypertension: efficacy and safety
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Francesco Puppo, Francesca Spanò, and Giuseppe Murdaca
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medicine.medical_specialty ,Ambrisentan ,Sildenafil ,Hypertension, Pulmonary ,Systemic sclerosis-related pulmonary arterial hypertension ,Prostanoids ,Anti-endothelin-1 ,Phosphodiesterase type 5 inhibitors ,TNF-α inhibitors ,Antihypertensive Agents ,Familial Primary Pulmonary Hypertension ,Humans ,Scleroderma, Systemic ,Pharmacology (medical) ,Scleroderma ,chemistry.chemical_compound ,Internal medicine ,medicine ,Adverse effect ,business.industry ,Systemic ,Pulmonary ,General Medicine ,medicine.disease ,Connective tissue disease ,Tadalafil ,Bosentan ,respiratory tract diseases ,Surgery ,chemistry ,Hypertension ,Cardiology ,business ,medicine.drug ,Iloprost ,Treprostinil - Abstract
Systemic sclerosis (SSc) is a rare connective tissue disease characterized by chronic inflammation and fibrosis of the skin, vascular abnormalities and variable involvement of organs. Patients with limited SSc typically develop pulmonary arterial hypertension (PAH). TNF-α, VEGF, platelet-derived growth factor and endothelin-1 play a key role in the development of PAH.This paper addresses the efficacy and safety of current drugs used for the treatment of PAH.Bosentan, ambrisentan, sildenafil, tadalafil, iloprost, epoprostenol and treprostinil were associated with hemodynamic improvements in PAH patients. Ambrisentan has a better safety profile compared with bosentan, regarding the risk of increase in hepatic transaminases. Flushing, dyspepsia and diarrhea were the most frequent adverse events in patients treated with sildenafil, while headache, myalgia and flushing were the adverse events in those receiving tadalafil. Inhaled iloprost is also effective, but it requires multiple daily nebulizations up to 15 min each and may induce cough, flushing, jaw pain and headache. Epoprostenol is considered the most effective approved therapy for severe PAH in WHO functional class III and class IV. TNF-α inhibitors reduce the systemic inflammation in patients with chronic immune-mediated diseases and improve the endothelial function, decreasing the risk of PAH progression.
- Published
- 2014
15. Long-term treatment of rheumatoid arthritis with adalimumab
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Francesco Puppo, Giuseppe Murdaca, and Francesca Spanò
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musculoskeletal diseases ,safety ,rheumatoid arthritis ,efficacy ,Review ,immunogenicity ,Etanercept ,Psoriatic arthritis ,Rheumatology ,Psoriasis ,adalimumab ,medicine ,Adalimumab ,infections ,Certolizumab pegol ,skin and connective tissue diseases ,Efficacy ,Immunogenicity ,Infections ,Rheumatoid arthritis ,Safety ,VEGF ,business.industry ,medicine.disease ,Infliximab ,Golimumab ,humanities ,Immunology ,business ,medicine.drug - Abstract
Rheumatoid arthritis (RA) is a chronic inflammatory disease that is associated with joint damage and progressive disability, an increased risk of morbidity related to comorbid conditions and substantial socioeconomic costs. Tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine known to have a central role in the initial host response to infection and in the pathogenesis of various immune-mediated diseases, such as RA, ankylosing spondylitis, psoriasis and/or psoriatic arthritis, Crohn's disease, and systemic lupus erythematosus. Five TNF-α inhibitors are available for the clinical use: infliximab; adalimumab; etanercept; golimumab; and certolizumab pegol. Infliximab is a chimeric human/murine IgG1 monoclonal antibody (mAb); adalimumab, and golimumab are human mAbs; certolizumab pegol is composed of the fragment antigen-binding anti-binding domain of a humanized anti-TNF-α mAb, combined with polyethylene glycol to increase its half-life in the body; etanercept is a fusion protein that acts as a "decoy receptor" for TNF-α. In this paper, we will briefly review the current data on efficacy and safety of adalimumab in patients with RA, its potential beneficial effects upon comorbid conditions, such as endothelial dysfunction and accelerated atherosclerosis in RA, and the immunogenicity.
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- 2013
16. Efficacy of cilostazol for the treatment of Raynaud’s phenomenon in systemic sclerosis patients
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Simone Negrini, Daniela Rollando, Elena Penza, Francesca Spanò, Francesco Puppo, Gilberto Filaci, and Francesco Indiveri
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Adult ,Genetics and Molecular Biology (all) ,medicine.medical_specialty ,Cilostazol ,Raynaud phenomenon ,Scleroderma ,Systemic sclerosis ,Medicine (all) ,Biochemistry, Genetics and Molecular Biology (all) ,Vasodilator Agents ,Tetrazoles ,030204 cardiovascular system & hematology ,Gastroenterology ,Biochemistry ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Maintenance therapy ,Surveys and Questionnaires ,medicine.artery ,Internal medicine ,Antithrombotic ,medicine ,Humans ,Brachial artery ,Interleukin 6 ,Aged ,030203 arthritis & rheumatology ,Scleroderma, Systemic ,Hematology ,biology ,business.industry ,Raynaud Disease ,General Medicine ,Plasma levels ,Middle Aged ,Endothelin 1 ,Surgery ,Treatment Outcome ,Controlled Before-After Studies ,biology.protein ,business ,medicine.drug - Abstract
Cilostazol is a selective inhibitor of phosphodiesterase-III with antiplatelet, antithrombotic and vasodilating properties. The aim of our study was to evaluate the effect of the drug on vasculopathy and Raynaud's phenomenon (RP), in a series of patients with systemic sclerosis (SSc), before and after cilostazol treatment. Twenty-one consecutive SSc patients with moderate or severe RP were enrolled in an open-label study. Cilostazol was administered at the dose of 100 mg twice a day, for 12 months. Evaluations included: daily RP attack diary documenting the frequency and duration of RP episodes, Health Assessment Questionnaire-Disability Index, scleroderma visual analogue scales (VAS), flow-mediated dilation and immunological status, including endothelin 1 and interleukin 6 plasma levels. Thirteen patients completed the study. RP duration and daily number episodes recorded over a 3-week period significantly decreased after cilostazol treatment (p = 0.0049 and p = 0.0067, respectively). VAS score indicated a significant amelioration of the patients' perception of RP (p = 0.0117), and both baseline and post-ischemic brachial artery diameters were significantly increased after cilostazol treatment, as compared with basal values (p = 0.0119 and p = 0.0076, respectively). None of the patients developed digital ulcers during the study. A significant clinical improvement of RP was recorded in SSc patients undergoing cilostazol treatment. Study results indicate a potential role of cilostazol as oral maintenance therapy in SSc patients with RP.
- Published
- 2016
17. Current Views on Diagnostic Approach and Treatment of Lymphedema
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Paola Cagnati, Giuseppe Murdaca, Francesca Spanò, Corradino Campisi, Francesco Puppo, Rossella Gulli, and Francesco Boccardo
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medicine.medical_specialty ,Primary lymphedema ,diagnosis ,Secondary lymphedema ,medicine.medical_treatment ,Postoperative Complications ,secondary lymphedema ,treatment ,Breast cancer ,hemic and lymphatic diseases ,medicine ,Humans ,Lymphedema ,Stage (cooking) ,business.industry ,General Medicine ,medicine.disease ,humanities ,Surgery ,Lymphangiogenesis ,body regions ,Radiation therapy ,Lymphatic system ,Chronic Disease ,Radiology ,medicine.symptom ,business - Abstract
Lymphedema is a chronic, progressive, and often debilitating condition. Primary lymphedema is a lymphatic malformation developing during the later stage of lymphangiogenesis. Secondary lymphedema is the result of obstruction or disruption of the lymphatic system, which can occur as a consequence of tumors, surgery, trauma, infection, inflammation, and radiation therapy. In this review, we report an update upon the diagnostic approach and the medical and surgical therapy for both primary and secondary lymphedema.
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- 2012
18. Immunogenicity of infliximab and adalimumab: what is its role in hypersensitivity and modulation of therapeutic efficacy and safety?
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Ottavia Magnani, Elena Penza, Francesca Spanò, Giuseppe Murdaca, Francesco Puppo, Andrea Guastalla, and Miriam Contatore
- Subjects
musculoskeletal diseases ,0301 basic medicine ,hypersensitivity reactions ,immune-mediated diseases ,immunogenicity ,Receptors, Tumor Necrosis Factor ,03 medical and health sciences ,Psoriatic arthritis ,0302 clinical medicine ,immune system diseases ,Psoriasis ,medicine ,Adalimumab ,Humans ,Pharmacology (medical) ,Hypersensitivity, Delayed ,Certolizumab pegol ,skin and connective tissue diseases ,adalimumab ,infliximab ,030203 arthritis & rheumatology ,Dose-Response Relationship, Drug ,business.industry ,Antibodies, Monoclonal ,General Medicine ,Immunoglobulin E ,medicine.disease ,humanities ,Infliximab ,Golimumab ,Antirheumatic Agents ,030104 developmental biology ,Immune System Diseases ,Rheumatoid arthritis ,Immunoglobulin G ,Immunology ,business ,medicine.drug - Abstract
TNF-α inhibitors have demonstrated efficacy both as monotherapy and in combination with disease-modifying antirheumatic drugs (DMARDs) in the treatment of chronic inflammatory immune-mediated diseases such as rheumatoid arthritis, Crohn's disease, ankylosing spondylitis, psoriasis and/or psoriatic arthritis, and may be administered off-label to treat disseminated granuloma annulare systemic lupus erythematosus and systemic sclerosis. There are several TNF-α inhibitors available for clinical use including infliximab, adalimumab, golimumab, certolizumab pegol and etanercept.infliximab and adalimumab can induce the development of anti-infliximab (anti-IFX) and anti-adalimumab (anti-ADA) monoclonal antibodies (mAbs). In this review, we discuss the impact of anti-IFX and anti-ADA mAbs upon efficacy and safety of these biological agents.IgG/IgE neutralizing antibodies against infliximab and adalimumab decrease the possibility of achieving a minimal disease activity state or clinical remission, decrease drug survival, increase the need for doctors to prescribe a higher drug dosage and, finally, favor the occurrence of adverse events. Concomitant administration of DMARDs such as methotrexate or leflunomide prevents the development of neutralizing Abs against infliximab and adalimumab.
- Published
- 2015
19. [Syncope: an untreated symptom with a lifesaving intervention]
- Author
-
Luca, Giupponi, Benedetta, De Chiara, Francesca, Spanò, Cristina, Giannattasio, Corrado, Taglieri, and Antonella, Moreo
- Subjects
Heart Neoplasms ,Treatment Outcome ,Aortic Valve ,Heart Valve Diseases ,Humans ,Female ,Fibroma ,Middle Aged ,Echocardiography, Transesophageal ,Syncope - Abstract
We report the case of a 61-year-old woman referred to our center for cardiac evaluation after a syncope, with echocardiographic findings of a papillary fibroelastoma on the edge of the non-coronary aortic cusp. The three-dimensional transesophageal approach provided a unique understanding of the size and shape of the mass and it favorably directed the surgeon towards treatment with conservative surgery.
- Published
- 2015
20. Harmful Effects on African Clawed Frog (Xenopus laevis) Reproduction as Expression of High Water Phosphates Levels
- Author
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Francesca Spanò, Maddalena Iannaccone, and Marco Campolo
- Subjects
African clawed frog ,biology ,media_common.quotation_subject ,Xenopus ,Zoology ,Reproduction ,biology.organism_classification ,media_common - Published
- 2015
21. Autoimmune central diabetes insipidus in a patient with ureaplasma urealyticum infection and review on new triggers of immune response
- Author
-
Francesca Spanò, Manuela Albertelli, Annamaria De Bellis, Rodolfo Russo, Diego Ferone, Miriam Contatore, Andrea Guastalla, Francesco Puppo, Giacomo Garibotto, Angelo Schenone, Giuseppe Murdaca, Murdaca, Giuseppe, Russo, Rodolfo, Spanã², Francesca, Ferone, Diego, Albertelli, Manuela, Schenone, Angelo, Contatore, Miriam, Guastalla, Andrea, De Bellis, Annamaria, Garibotto, Giacomo, and Puppo, Francesco
- Subjects
Protein Precursor ,genetic structures ,Vasopressins ,Endocrinology, Diabetes and Metabolism ,Neurophysin ,lcsh:Medicine ,medicine.disease_cause ,urologic and male genital diseases ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Arthritis, Reactive ,Autoimmune Disease ,Autoimmune Diseases ,Ureaplasma Infection ,Polyuria ,Langerhans cell histiocytosis ,medicine ,Humans ,Protein Precursors ,Autoantibodies ,Neurophysins ,lcsh:RC648-665 ,business.industry ,Ureaplasma Infections ,Ureaplasma infection ,Medicine (all) ,lcsh:R ,Autoantibody ,Middle Aged ,medicine.disease ,Nephrogenic diabetes insipidus ,Autoantibodie ,female genital diseases and pregnancy complications ,Diabetes Insipidus, Neurogenic ,Immunology ,Diabetes insipidus ,Female ,Sarcoidosis ,medicine.symptom ,business ,Ureaplasma urealyticum ,hormones, hormone substitutes, and hormone antagonists ,Vasopressin ,Human - Abstract
Diabetes insipidus is a disease in which large volumes of dilute urine (polyuria) are excreted due to vasopressin (AVP) deficiency [central diabetes insipidus (CDI)] or to AVP resistance (nephrogenic diabetes insipidus). In the majority of patients, the occurrence of CDI is related to the destruction or degeneration of neurons of the hypothalamic supraoptic and paraventricular nuclei. The most common and well recognized causes include local inflammatory or autoimmune diseases, vascular disorders, Langerhans cell histiocytosis (LCH), sarcoidosis, tumors such as germinoma/craniopharyngioma or metastases, traumatic brain injuries, intracranial surgery, and midline cerebral and cranial malformations. Here we have the opportunity to describe an unusual case of female patient who developed autoimmune CDI following ureaplasma urealyticum infection and to review the literature on this uncommon feature. Moreover, we also discussed the potential mechanisms by which ureaplasma urealyticum might favor the development of autoimmune CDI.
- Published
- 2015
22. Paroxysmal supraventricular tachycardia as first manifestation of right atrial hemangioma during endovascular treatment of intracranial arteriovenous fistulas
- Author
-
Alberto Cereda, Alberto Roghi, Antonella Moreo, Patrizia Pedrotti, Edgardo Bonacina, Cristina Giannattasio, Francesca Spanò, Angelica Peritore, Spanò, F, Cereda, A, Moreo, A, Bonacina, E, Peritore, A, Roghi, A, Giannattasio, C, and Pedrotti, P
- Subjects
Male ,cerebral fistulas ,medicine.medical_specialty ,atrial mass ,Mixed type ,Arteriovenous fistula ,Paroxysmal supraventricular tachycardia ,Right atrial ,cardiac imaging ,Hemangioma ,cerebral fistula ,medicine ,Tachycardia, Supraventricular ,Humans ,cardiovascular diseases ,Heart Atria ,Endovascular treatment ,paroxysmal supraventricular tachycardia ,Cardiac imaging ,Ultrasonography ,business.industry ,mixed type cavernous-capillary hemangioma ,Cardiac hemangioma ,Endovascular Procedures ,Middle Aged ,medicine.disease ,Research Paper: Pathology ,Surgery ,Oncology ,atrial ma ,Arteriovenous Fistula ,cardiovascular system ,business - Abstract
We report the description of a cardiac mass occupying almost the entire right atrium in a young man who developed paroxysmal supraventricular tachycardia during endovascular treatment of intracranial arteriovenous fistulas. The mass was detected at echocardiographic examination, its tissue characteristics were defined with cardiac magnetic resonance and it was successfully surgically removed. The histopathological findings were consistent with a mixed type cavernous-capillary hemangioma of the heart. The intriguing co-existence of cardiac hemangioma and cerebral arteriovenous fistulas, to the best of our knowledge, has not been previously reported in English Literature.
- Published
- 2015
23. Pharmacogenetics and future therapeutic scenarios: what affects the prediction of response to treatment with etanercept?
- Author
-
Giuseppe, Murdaca, Rossella, Gulli, Francesca, Spanò, Paola, Mandich, and Francesco, Puppo
- Subjects
Arthritis, Rheumatoid ,Treatment Outcome ,Genotype ,Tumor Necrosis Factor-alpha ,Antirheumatic Agents ,Immunoglobulin G ,Humans ,Lymphotoxin-alpha ,Polymorphism, Single Nucleotide ,Receptors, Tumor Necrosis Factor ,Etanercept - Abstract
There are five tumor necrosis factor alpha (TNF-α) inhibitors available for clinical use that have demonstrated efficacy as monotherapy or in combination with other anti-inflammatory or disease-modifying anti-rheumatic drugs (DMARDs) in the treatment of immune-mediated diseases. These include the anti-TNF-α monoclonal antibodies infliximab, adalimumab, golimumab, and certolizumab pegol, and the fusion protein, etanercept. The use of pharmacogenetic testing has the potential to increase drug efficiency by identifying genetic factors responsible for a lack of response to, or toxicities from, TNF-α inhibitors, and could be used to individualize therapy. Several studies have reported associations between genetic polymorphisms and the response to etanercept, but most are small and insufficiently powered to detect effect, and markers tend to be more prognostic than predictive of therapeutic response. Limitations of pharmacogenetic studies include the use of single nucleotide polymorphisms (SNPs), genes in linkage with other loci, interaction of environmental factors, and cohort heterogeneity, all of which can complicate the relationship between genetic polymorphisms and treatment response. Further studies are needed for pharmacogenetics to become a routine part of daily clinical therapeutic practice.
- Published
- 2014
24. Potential use of TNF-α inhibitors in systemic sclerosis
- Author
-
Andrea Guastalla, Miriam Contatore, Francesco Puppo, Francesca Spanò, and Giuseppe Murdaca
- Subjects
Inflammatory arthritis ,Hypertension, Pulmonary ,Pulmonary Fibrosis ,Immunology ,Inflammation ,Opportunistic Infections ,Systemic inflammation ,Antibodies, Monoclonal, Humanized ,Receptors, Tumor Necrosis Factor ,Etanercept ,Polyethylene Glycols ,Immunoglobulin Fab Fragments ,Fibrosis ,Latent Tuberculosis ,Pulmonary fibrosis ,medicine ,Immunology and Allergy ,Humans ,skin and connective tissue diseases ,Scleroderma, Systemic ,integumentary system ,business.industry ,Tumor Necrosis Factor-alpha ,Arthritis ,Adalimumab ,Antibodies, Monoclonal ,medicine.disease ,Connective tissue disease ,Antibodies, Neutralizing ,Infliximab ,Oncology ,Antirheumatic Agents ,Immunoglobulin G ,Certolizumab Pegol ,Drug Evaluation ,Drug Therapy, Combination ,Endothelium, Vascular ,medicine.symptom ,business ,Immunosuppressive Agents ,medicine.drug ,Forecasting - Abstract
Systemic sclerosis (SSc) is a rare connective tissue disease characterized by chronic inflammation and fibrosis of the skin, vascular abnormalities and variable involvement of organs. TNF-α has a central role in initial host response to infections and in the pathogenesis of various systemic immune-mediated diseases. Serum levels of TNF-α are elevated in patients with SSc and favor the development of pulmonary fibrosis and pulmonary arterial hypertension. Inflammatory arthritis can occur in patients with SSc. Infliximab and etanercept may improve the inflammatory arthritis and disability in SSc. TNF-α inhibitors reduce the systemic inflammation, improve the endothelial function decreasing the risk of pulmonary arterial hypertension progression and of acute cardiovascular and/or cerebrovascular events. Physicians need to be aware of the potential risks of tuberculosis reactivation and opportunistic infections. Randomized controlled trials with TNF-α inhibitors in patients with SSc are needed to confirm the potential role of these agents in the treatment of SSc.
- Published
- 2014
25. Pharmacogenetics of etanercept: role of TNF-α gene polymorphisms in improving its efficacy
- Author
-
Andrea Guastalla, Francesca Spanò, Ottavia Magnani, Miriam Contatore, Francesco Puppo, and Giuseppe Murdaca
- Subjects
Anti-Inflammatory Agents ,immune-mediated diseases ,Toxicology ,Polymorphism, Single Nucleotide ,Receptors, Tumor Necrosis Factor ,Etanercept ,Psoriatic arthritis ,Psoriasis ,Receptors ,medicine ,Animals ,Humans ,Precision Medicine ,Polymorphism ,Granuloma annulare ,TNF-α inhibitors ,Pharmacology ,Ankylosing spondylitis ,TNF-α gene polymorphisms ,business.industry ,Tumor Necrosis Factor-alpha ,Patient Selection ,etanercept ,Immunoglobulin G ,Individualized Medicine ,Phenotype ,Pharmacogenetics ,General Medicine ,Single Nucleotide ,medicine.disease ,Clinical trial ,Rheumatoid arthritis ,Immunology ,business ,Tumor Necrosis Factor ,medicine.drug - Abstract
During the last decade, many new biological immune modulators have entered the market as new therapeutic principles. Biologics, including TNF-α inhibitors, are the new frontier in the treatment of immune-mediated or inflammatory diseases, such as rheumatoid arthritis, systemic lupus erythematosus, Crohn's disease, ankylosing spondylitis, systemic sclerosis, disseminated granuloma annulare, psoriasis and/or psoriatic arthritis. TNF-α inhibitors have demonstrated efficacy and are well tolerated in large, randomized, controlled clinical trials. However, a substantial proportion of patients do not respond to these agents and potential adverse drug reactions may be associated with its use.Pharmacogenetics has the potential of increasing drug efficiency by identifying genetic factors responsible for lack of response or toxicities to TNF-α inhibitors. In this review, we analyze the influence of several polymorphisms upon the efficacy and safety of TNF-α inhibitors.Several polymorphisms have been proven to influence the response to etanercept. Among them, single nucleotide polymorphisms (SNPs) -308 G/G, -857 C/T, +489 GG and GA, HLA-DRB1-encoding SE (allele *0404 and allele *0101) favor the response to etanercept, whereas SNP -308 A/A and TNFR1A AA decrease the response. Large clinical studies are needed to confirm the relevance of these associations in order to tailor treatment and to decrease unnecessary toxicity.
- Published
- 2014
26. Efficacy and safety of etanercept in chronic immune-mediated disease
- Author
-
Andrea Guastalla, Francesca Spanò, Giuseppe Murdaca, Miriam Contatore, Francesco Puppo, and Ottavia Magnani
- Subjects
musculoskeletal diseases ,Polymyositis ,Receptors, Tumor Necrosis Factor ,Autoimmune Diseases ,Etanercept ,Psoriatic arthritis ,Mixed connective tissue disease ,immune system diseases ,medicine ,Humans ,Pharmacology (medical) ,skin and connective tissue diseases ,Ankylosing spondylitis ,Tumor Necrosis Factor-alpha ,business.industry ,General Medicine ,Dermatomyositis ,medicine.disease ,Infliximab ,stomatognathic diseases ,Antirheumatic Agents ,Immunoglobulin G ,Rheumatoid arthritis ,Chronic Disease ,Immunology ,business ,Immunosuppressive Agents ,medicine.drug - Abstract
TNF-α inhibitors have demonstrated efficacy in large, randomized controlled clinical trials either as monotherapy or in combination with other anti-inflammatory or disease-modifying antirheumatic drugs in the treatment of chronic inflammatory immune-mediated diseases. Etanercept is a fusion protein that acts as a 'decoy receptor' for TNF-α.This paper evaluates the efficacy and safety of etanercept in patients with chronic inflammatory immune-mediated diseases.Etanercept was first approved for the treatment of rheumatoid arthritis (RA) and subsequently of chronic plaque psoriasis, psoriatic arthritis, ankylosing spondylitis and juvenile RA. Etanercept as other TNF-α inhibitors, particularly infliximab, may be administered off-label to treat other chronic inflammatory immune-mediated diseases such as systemic sclerosis, Behcet disease, systemic lupus erythematosus, polymyositis, dermatomyositis and mixed connective tissue disease. Early etanercept treatment prevents joint damage and helps to avoid long-term disability in arthritis. Etanercept administered at a dose of 50 mg once weekly is effective in inducing an earlier remission of RA, and etanercept 50 mg twice weekly may favor a more rapid improvement of psoriasis and psoriatic arthritis. Etanercept and adalimumab may exert beneficial effects on lipid profile and improve endothelial dysfunction. Appropriate screening tests for latent tuberculosis, hepatitis B virus and hepatitis C virus should be performed before starting etanercept. TNF-α inhibitors including etanercept are contraindicated in patients with demyelinating diseases.
- Published
- 2014
27. TNF-α gene polymorphisms: association with disease susceptibility and response to anti-TNF-α treatment in psoriatic arthritis
- Author
-
Martina Burlando, Giuseppe Murdaca, Francesca Spanò, Aurora Parodi, Francesca Lantieri, Paola Mandich, Rossella Gulli, and Francesco Puppo
- Subjects
Male ,Candidate gene ,Arthritis ,Psoriatic ,Biochemistry ,Severity of Illness Index ,Receptors, Tumor Necrosis Factor ,Etanercept ,Monoclonal ,Receptors ,Humanized ,Medicine (all) ,Antibodies, Monoclonal ,Single Nucleotide ,Middle Aged ,Treatment Outcome ,Female ,medicine.drug ,Adult ,Genotype ,Single-nucleotide polymorphism ,Dermatology ,Antibodies, Monoclonal, Humanized ,Polymorphism, Single Nucleotide ,Antibodies ,Psoriatic arthritis ,Adalimumab ,Alleles ,Arthritis, Psoriatic ,Case-Control Studies ,Genetic Predisposition to Disease ,Humans ,Immunoglobulin G ,Infliximab ,Tumor Necrosis Factor-alpha ,2708 ,Cell Biology ,Molecular Biology ,Psoriasis Area and Severity Index ,medicine ,Polymorphism ,business.industry ,medicine.disease ,Immunology ,business ,Tumor Necrosis Factor - Abstract
The tumor necrosis factor-α ( TNF-α ) gene has been proposed as a major candidate gene in psoriatic arthritis (PsA). TNF-α is a therapeutic target for patients responding poorly to conventional treatments. We investigated the role of single-nucleotide polymorphisms (SNPs) at positions -238, -308, and +489 of the TNF-α gene in the genetic susceptibility to PsA, in the severity of the disease, and, finally, in the response to TNF-α inhibitors (adalimumab, etanercept, or infliximab). Fifty-seven Caucasian PsA patients and 155 healthy matched controls were studied. The SNP +489 variant allele A was significantly associated with PsA susceptibility ( P =0.0136) and severity of clinical (Psoriasis Area and Severity Index score, American College of Rheumatology criteria, Disease Activity Score 28, and Disability Index Health Assessment Questionnaire) and laboratory (C-reactive protein and erythrocyte sedimentation rate) parameters ( P -values ranging from 0.016 to 2.908 × 10 −12 ). The difference in severity was accounted for by the differences between the AA and GA genotypes with respect to the GG genotype. The SNP +489A allele shows a trend of association with the response to PsA treatment with etanercept. These findings suggest a role of the SNP +489A allele in the susceptibility and severity of PsA.
- Published
- 2013
28. Free radicals and endothelial dysfunction: potential positive effects of TNF-α inhibitors
- Author
-
Giuseppe Murdaca, Francesco Puppo, Paola Cagnati, and Francesca Spanò
- Subjects
Vascular Endothelial Growth Factor A ,Physiology ,medicine.medical_treatment ,Clinical Biochemistry ,Bradykinin ,Inflammation ,Biology ,Pharmacology ,Nitric Oxide ,Biochemistry ,chemistry.chemical_compound ,medicine ,Humans ,Obesity ,Vascular Diseases ,Endothelial dysfunction ,Interleukin 6 ,Review Articles ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Biochemistry (medical) ,Cell Biology ,medicine.disease ,Atherosclerosis ,Nitric oxide synthase ,Vascular endothelial growth factor A ,Oxidative Stress ,Cytokine ,chemistry ,Immunology ,biology.protein ,Tumor necrosis factor alpha ,Endothelium, Vascular ,medicine.symptom ,Nitric Oxide Synthase ,Biomarkers - Abstract
Objectives During the last decade many new biological immune modulators have entered the market as new therapeutic principles. Tumor necrosis factor (TNF)-α is a pro-inflammatory cytokine known to a have a key role in the pathogenic mechanisms of various immune-mediated or inflammatory diseases. However, TNF-α also plays a key role in endothelial dysfunction and, thus, in the development and progression of atherosclerosis. What, then, is the potential therapeutic role of TNF-α inhibitors? Methods We analysed the current literature concerning the administration of TNF-α inhibitors and their potential benefits upon endothelial function. Results TNF-α inhibitors decrease the serum levels of inflammatory markers such as TNF-α itself, CRP, IL-6, and increased the tissue expression of endothelial NO synthase and the vasodilatory response to bradykinin. Discussion TNF-α inhibitors may change the progression of endothelial dysfunction and, thus, slow down the atherosclerotic process.
- Published
- 2013
29. Effects of TNF-α inhibitors upon the mechanisms of action of VEGF
- Author
-
Maurizio Miglino, Francesco Puppo, Giuseppe Murdaca, and Francesca Spanò
- Subjects
musculoskeletal diseases ,Vascular Endothelial Growth Factor A ,Immunology ,Anti-Inflammatory Agents ,Angiogenesis Inhibitors ,Pharmacology ,Antibodies, Monoclonal, Humanized ,Etanercept ,Psoriatic arthritis ,Psoriasis ,Adalimumab ,medicine ,Immunology and Allergy ,Humans ,Certolizumab pegol ,skin and connective tissue diseases ,Neovascularization, Pathologic ,business.industry ,Tumor Necrosis Factor-alpha ,Antibodies, Monoclonal ,medicine.disease ,humanities ,Infliximab ,Golimumab ,Oncology ,Rheumatoid arthritis ,business ,medicine.drug - Abstract
TNF-a is a proinflammatory cytokine known to a have a central role in the initial host response to infection [1–5] and the pathogenesis of various immune-mediated diseases such as rheumatoid arthritis (RA), systemic lupus erythematosus, ankylosing spondylitis (AS), psoriasis (Ps) and/or psoriatic arthritis (PsA), as well as granuloma annulare [1–5]. TNF-a inhibitors have demonstrated efficacy in large, randomized, controlled clinical trials either as monotherapy or in combination with other anti-inflammatory or disease-modifying antirheumatic drugs [1–4]. Five TNF-a inhibitors are available for clinical use: infliximab, adalimumab, golimumab, certolizumab pegol and etanercept [1–5]. Infliximab is a chimeric human/murine IgG1 monoclonal antibody (mAb), adalimumab and golimumab are human mAbs, certolizumab pegol is composed of the Fab antigen-binding domain of a humanized anti-TNF-a mAb combined with polyethylene glycol to increase its half-life in the body and etanercept is a fusion protein that acts as a ‘decoy receptor’ for TNF-a [1–5].
- Published
- 2013
30. Use of leflunomide plus TNF-α inhibitors in rheumatoid arthritis
- Author
-
Francesca Spanò, Francesco Puppo, and Giuseppe Murdaca
- Subjects
musculoskeletal diseases ,Leflunomide ,Opportunistic infections ,Rheumatoid arthritis ,TNF-α inhibitors ,Anti-Inflammatory Agents ,Antirheumatic Agents ,Arthritis, Rheumatoid ,Drug Therapy, Combination ,Humans ,Isoxazoles ,Tumor Necrosis Factor-alpha ,Pharmacology (medical) ,Arthritis ,Pharmacology ,Etanercept ,Drug Therapy ,Rheumatoid ,medicine ,Adalimumab ,Certolizumab pegol ,skin and connective tissue diseases ,business.industry ,General Medicine ,medicine.disease ,Golimumab ,Infliximab ,Combination ,business ,medicine.drug - Abstract
Rheumatoid arthritis (RA) is a chronic inflammatory immune-mediated systemic disease which primarily affects the joints. Leflunomide (LFN) is a disease modifying anti-rheumatic drugs (DMARDs) which acts by inhibiting the synthesis of pyrimidines. Several trials have demonstrated the efficacy and safety of LFN alone or in combination with biological agents such as tumor necrosis factor-α (TNF-α) inhibitors in the treatment of RA patients. TNF-α is a pro-inflammatory cytokine known to a have a key role in the pathogenesis of RA. TNF-α inhibitors have demonstrated efficacy as monotherapy or in combination with other anti-inflammatory or DMARDs in the treatment of RA. Five TNF-α inhibitors are available for clinical use and include infliximab, adalimumab, golimumab, certolizumab pegol and etanercept. In this editorial, we briefly discuss the efficacy and safety of LFN and TNF-α inhibitors in the treatment of RA, and the potential beneficial effect of both LFN and TNF-α inhibitors in improving the endothelial dysfunction and in reducing the risk of acute cardiovascular and/or cerebrovascular events.
- Published
- 2013
31. Selective TNF-α inhibitor-induced injection site reactions
- Author
-
Francesco Puppo, Francesca Spanò, and Giuseppe Murdaca
- Subjects
medicine.medical_treatment ,Pharmacology ,Antibodies, Monoclonal, Humanized ,Dermatitis, Contact ,Infusions, Subcutaneous ,Receptors, Tumor Necrosis Factor ,Etanercept ,Adalimumab ,medicine ,Animals ,Humans ,Pharmacology (medical) ,Adverse effect ,Inflammation ,Tumor Necrosis Factor-alpha ,business.industry ,General Medicine ,medicine.disease ,Clinical trial ,Cytokine ,Antirheumatic Agents ,Immunoglobulin G ,Monoclonal ,Eosinophilic cellulitis ,Immunology ,Inflammation Mediators ,business ,Adverse drug reaction ,medicine.drug - Abstract
During the last decade, many new biological immune modulators entered the market as new therapeutic principles. TNF-α is a pro-inflammatory cytokine known to a have a key role in the pathogenic mechanisms of various immune-mediated or inflammatory diseases. TNF-α blockers have demonstrated efficacy in large, randomized controlled clinical trials either as monotherapy or in combination with other anti-inflammatory or disease-modifying anti-rheumatic drugs.Although generally well tolerated and safe, potential adverse events may be associated with TNF-α inhibitor treatment. The authors will briefly review the potential adverse drug reactions and the immunological mechanisms of injection site reactions (ISRs) in patients treated with etanercept and adalimumab.Patients treated with TNF-α inhibitors can develop ISR around the sites of injections. 'Type IV delayed type reaction' or 'recall ISRs'. Eosinophilic cellulitis or 'Wells syndrome', 'type III' and 'type I' reactions are reported. Long-term studies are necessary to determine the durability of response and the real risk of ISRs with golimumab and certolizumab pegol. Further studies are also necessary to evaluate the immunogenicity of these drugs.
- Published
- 2013
32. Endothelial dysfunction in rheumatic autoimmune diseases
- Author
-
Paola Cagnati, Giuseppe Murdaca, Francesca Spanò, Barbara Maria Colombo, Rossella Gulli, and Francesco Puppo
- Subjects
Endothelium ,Endothelial dysfunction, Atherosclerosis, Autoimmune diseases ,Autoimmune diseases ,Adaptive Immunity ,Autoantigens ,Immune system ,Antiphospholipid syndrome ,Rheumatic Diseases ,Medicine ,Animals ,Humans ,Endothelial dysfunction ,Dyslipidemias ,Innate immune system ,business.industry ,Autoantibody ,medicine.disease ,Acquired immune system ,Atherosclerosis ,Immunity, Innate ,Oxidative Stress ,medicine.anatomical_structure ,Rheumatoid arthritis ,Immunology ,Endothelium, Vascular ,Inflammation Mediators ,Cardiology and Cardiovascular Medicine ,business ,Immunosuppressive Agents - Abstract
Rheumatic autoimmune diseases have been associated with accelerated atherosclerosis and various types of vasculopathies. Atherosclerosis is an inflammatory condition which starts as a "response to injury" favoring endothelial dysfunction which is associated with increased expression of adhesion molecules, pro-inflammatory cytokines, pro-thrombotic factors, oxidative stress upregulation and abnormal vascular tone modulation. Endothelial dysfunction in rheumatic autoimmune diseases involves innate immune responses, including macrophages and dendritic cells expression of scavenger and toll-like receptors for modified or native LDL as well as neutrophil and complement activation, and dysregulation of adaptive immune responses, including proliferation of autoreactive T-helper-1 lymphocytes and defective function of dendritic and regulatory T cells. Specific differences for endothelial function among different disorders include: a) increased amounts of pro-atherogenic hormones, decreased amounts of anti-atherogenic hormones and increased insulin resistance in rheumatoid arthritis; b) autoantibodies production in systemic lupus erythematosus and antiphospholipid syndrome; c) smooth muscle cells proliferation, destruction of internal elastic lamina, fibrosis and coagulation and fibrinolytic system dysfunction in systemic sclerosis. Several self-antigens (i.e. high density lipoproteins, heat shock proteins, β2-glycoprotein1) and self-molecules modified by oxidative events (i.e. low density lipoproteins and oxidized hemoglobin) have been identified as targets of autoimmune responses. Endothelial dysfunction leads to accelerated atherosclerosis in rheumatoid arthritis, systemic lupus erythematosus and spondyloarthropaties whereas obliterative vasculopathy is associated with systemic sclerosis. In this paper, we will briefly review the most relevant information upon endothelial dysfunction and inflammatory mechanisms in atherosclerosis and we will summarize the similarities and differences in vascular disease patterns underlying different rheumatic autoimmune diseases.
- Published
- 2012
33. Update upon efficacy and safety of TNF-α inhibitors
- Author
-
Paola Cagnati, Francesca Spanò, Barbara Maria Colombo, Giuseppe Murdaca, Rossella Gulli, and Francesco Puppo
- Subjects
musculoskeletal diseases ,Anti-Inflammatory Agents ,Pharmacology ,Bioinformatics ,Etanercept ,Adalimumab ,medicine ,Humans ,Immunologic Factors ,Pharmacology (medical) ,Certolizumab pegol ,Adverse effect ,Inflammation ,business.industry ,Tumor Necrosis Factor-alpha ,Antibodies, Monoclonal ,General Medicine ,humanities ,Golimumab ,Infliximab ,Tnf α inhibitors ,Immune System Diseases ,Tumor necrosis factor alpha ,Female ,business ,medicine.drug - Abstract
The ongoing progresses in the knowledge of the pathogenic mechanisms of various immune-mediated and inflammatory diseases as well as the availability of innovative biotechnological approaches have led to the development of new drugs that add to conventional treatments. Among these, tumor necrosis factor (TNF)-α inhibitors, that is, infliximab, adalimumab, etanercept, golimumab and certolizumab pegol, are now available for clinical use. This editorial discusses the recent indications of TNF-α inhibitors, the pretreatment considerations, the reported adverse events and, finally, the recommendations for its use in pregnancy.
- Published
- 2011
34. Soluble human leukocyte antigen–G serum levels in patients with acquired immune deficiency syndrome affected by different disease-defining conditions before and after antiretroviral treatment
- Author
-
Paola Cagnati, Francesca Spanò, Maurizio Setti, Paola Contini, Francesco Puppo, Francesca Lantieri, and Giuseppe Murdaca
- Subjects
CD4-Positive T-Lymphocytes ,Immunology ,Disease ,Human leukocyte antigen ,CD8-Positive T-Lymphocytes ,Virus Replication ,Biomarkers, Pharmacological ,Immune system ,Antigen ,Acquired immunodeficiency syndrome (AIDS) ,medicine ,Humans ,Immunology and Allergy ,Wasting Syndrome ,Sarcoma, Kaposi ,Kaposi's sarcoma ,Cells, Cultured ,HLA-G Antigens ,Acquired Immunodeficiency Syndrome ,AIDS-Related Opportunistic Infections ,business.industry ,HIV ,General Medicine ,medicine.disease ,RNA, Viral ,business ,Biomarkers ,CD8 - Abstract
We have previously reported that the serum levels of soluble human leukocyte antigen (HLA)-A, -B, -C, and -G antigens are elevated in human immunodeficiency virus (HIV)-infected subjects and decrease after antiretroviral therapy. In this study, we measured soluble HLA-G serum levels in patients with acquired immune deficiency syndrome (AIDS) affected by different AIDS-defining conditions before and during antiretroviral therapy and correlated them with virologic and immunologic parameters of response to treatment. Soluble HLA-G levels were significantly higher in AIDS patients before treatment as compared with healthy controls and significantly decreased after 36 months of therapy. The decrease of soluble HLA-G correlated with the decrease of plasma HIV-RNA level and CD8(+) T-lymphocytes number and with the increase of CD4(+) T-lymphocytes number. Soluble HLA-G levels were significantly higher in patients with opportunistic infections and Kaposi's sarcoma compared with patients with the wasting syndrome. These data suggest that infections and neoplasms may trigger the shedding of soluble HLA-G molecules, and confirm that the level of soluble HLA-G in serum might represent a surrogate marker to monitor virologic response and immune reconstitution in HIV-positive individuals.
- Published
- 2011
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