9 results on '"Fourcot, Marie"'
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2. An extended transcription factor regulatory network controls hepatocyte identity
- Author
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Dubois‐Chevalier, Julie, primary, Gheeraert, Céline, additional, Berthier, Alexandre, additional, Boulet, Clémence, additional, Dubois, Vanessa, additional, Guille, Loïc, additional, Fourcot, Marie, additional, Marot, Guillemette, additional, Gauthier, Karine, additional, Dubuquoy, Laurent, additional, Staels, Bart, additional, Lefebvre, Philippe, additional, and Eeckhoute, Jérôme, additional
- Published
- 2023
- Full Text
- View/download PDF
3. An extended transcription factor regulatory network involving stabilization loops controls hepatocyte identity
- Author
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Dubois-Chevalier, Julie, Gheeraert, Céline, Berthier, Alexandre, Boulet, Clémence, Dubois, Vanessa, Guille, Loïc, Fourcot, Marie, Marot, Guillemette, Gauthier, Karine, Dubuquoy, Laurent, Staels, Bart, Lefebvre, Philippe, Eeckhoute, Jérôme, Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lille, CHU Lille, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Fondation Recherche Médicale (FRM grant EQU202203014645), ANR-21-CE14-0032,HSCreg,Identification et caractérisation d'un nouveau facteur de transcription controlant l'activation des cellules stellaires et la fibrose hépatique(2021), ANR-21-CE17-0016,MEdicAL,Cibler les MEcanismes cellulaires du défaut de régénération pour le développement d'une médecine de précision pour l'hépatite ALcoolique.(2021), ANR-10-LABX-0046,EGID,EGID Diabetes Pole(2010), and ANR-16-IDEX-0004,ULNE,ULNE(2016)
- Subjects
[SDV]Life Sciences [q-bio] - Published
- 2023
4. Hepatitis C virus alters the morphology and function of peroxisomes
- Author
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Martin de Fourchambault, Esther, Callens, Nathalie, Saliou, Jean Michel, Fourcot, Marie, Delos, Oceane, Barois, Nicolas, Thorel, Quentin, Ramirez, Santseharay, Bukh, Jens, Cocquerel, Laurence, Bertrand-Michel, Justine, Marot, Guillemette, Sebti, Yasmine, Dubuisson, Jean, Rouillé, Yves, Martin de Fourchambault, Esther, Callens, Nathalie, Saliou, Jean Michel, Fourcot, Marie, Delos, Oceane, Barois, Nicolas, Thorel, Quentin, Ramirez, Santseharay, Bukh, Jens, Cocquerel, Laurence, Bertrand-Michel, Justine, Marot, Guillemette, Sebti, Yasmine, Dubuisson, Jean, and Rouillé, Yves
- Abstract
Despite the introduction of effective treatments for hepatitis C in clinics, issues remain regarding the liver disease induced by chronic hepatitis C virus (HCV) infection. HCV is known to disturb the metabolism of infected cells, especially lipid metabolism and redox balance, but the mechanisms leading to HCV-induced pathogenesis are still poorly understood. In an APEX2-based proximity biotinylation screen, we identified ACBD5, a peroxisome membrane protein, as located in the vicinity of HCV replication complexes. Confocal microscopy confirmed the relocation of peroxisomes near HCV replication complexes and indicated that their morphology and number are altered in approximately 30% of infected Huh-7 cells. Peroxisomes are small versatile organelles involved among other functions in lipid metabolism and ROS regulation. To determine their importance in the HCV life cycle, we generated Huh-7 cells devoid of peroxisomes by inactivating the PEX5 and PEX3 genes using CRISPR/Cas9 and found that the absence of peroxisomes had no impact on replication kinetics or infectious titers of HCV strains JFH1 and DBN3a. The impact of HCV on peroxisomal functions was assessed using sub-genomic replicons. An increase of ROS was measured in peroxisomes of replicon-containing cells, correlated with a significant decrease of catalase activity with the DBN3a strain. In contrast, HCV replication had little to no impact on cytoplasmic and mitochondrial ROS, suggesting that the redox balance of peroxisomes is specifically impaired in cells replicating HCV. Our study provides evidence that peroxisome function and morphology are altered in HCV-infected cells.
- Published
- 2023
5. Hepatitis C virus alters the morphology and function of peroxisomes.
- Author
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de Fourchambault, Esther Martin, Callens, Nathalie, Saliou, Jean-Michel, Fourcot, Marie, Delos, Oceane, Barois, Nicolas, Thorel, Quentin, Ramirez, Santseharay, Bukh, Jens, Cocquerel, Laurence, Bertrand-Michel, Justine, Marot, Guillemette, Sebti, Yasmine, Dubuisson, Jean, and Rouillé, Yves
- Subjects
HEPATITIS C virus ,PEROXISOMES ,CHRONIC hepatitis C ,MORPHOLOGY ,LIFE cycles (Biology) ,MEMBRANE lipids - Abstract
Despite the introduction of effective treatments for hepatitis C in clinics, issues remain regarding the liver disease induced by chronic hepatitis C virus (HCV) infection. HCV is known to disturb the metabolism of infected cells, especially lipid metabolism and redox balance, but the mechanisms leading to HCV- induced pathogenesis are still poorly understood. In an APEX2-based proximity biotinylation screen, we identified ACBD5, a peroxisome membrane protein, as located in the vicinity of HCV replication complexes. Confocal microscopy confirmed the relocation of peroxisomes near HCV replication complexes and indicated that their morphology and number are altered in approximately 30% of infected Huh-7 cells. Peroxisomes are small versatile organelles involved among other functions in lipid metabolism and ROS regulation. To determine their importance in the HCV life cycle, we generated Huh-7 cells devoid of peroxisomes by inactivating the PEX5 and PEX3 genes using CRISPR/Cas9 and found that the absence of peroxisomes had no impact on replication kinetics or infectious titers of HCV strains JFH1 and DBN3a. The impact of HCV on peroxisomal functions was assessed using sub-genomic replicons. An increase of ROS was measured in peroxisomes of replicon-containing cells, correlated with a significant decrease of catalase activity with the DBN3a strain. In contrast, HCV replication had little to no impact on cytoplasmic and mitochondrial ROS, suggesting that the redox balance of peroxisomes is specifically impaired in cells replicating HCV. Our study provides evidence that peroxisome function and morphology are altered in HCV-infected cells. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
6. [Letter to editor] Altered microRNA expression in severe COVID‐19: Potential prognostic and pathophysiological role
- Author
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Garnier, Nathalie, Pollet, Kato, Fourcot, Marie, Caplan, Morgan, Marot, Guillemette, Goutay, Julien, Labreuche, Julien, Soncin, F., Boukherroub, Rabah, Hober, Didier, Szunerits, Sabine, Poissy, Julien, Engelmann, Ilka, Hospices Civils de Lyon (HCL), Ingénieries santé médicament, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Evaluation des technologies de santé et des pratiques médicales - ULR 2694 (METRICS), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), MOdel for Data Analysis and Learning (MODAL), Laboratoire Paul Painlevé (LPP), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Sciences et Technologies-Inria Lille - Nord Europe, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Evaluation des technologies de santé et des pratiques médicales - ULR 2694 (METRICS), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-École polytechnique universitaire de Lille (Polytech Lille), CHU Lille, Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] (UNICANCER/Lille), Université de Lille-UNICANCER, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Pathogenèse virale du diabète de type 1 - ULR 3610 (Laboratoire de Virologie), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), The authors thank B. Guerred, S. Menasria and S. Miloudi for excellent technical assistance. They acknowledge the work of technicians and engineers of the Biology and Pathology Center of the Lille University Hospital (CHU Lille) who are involved in the diagnostics of SARS-CoV-2. This research was supported by I-SITE ULNE, the Centre Hospitalier Universitaire de Lille and Université de Lille., and IEMN, Collection
- Subjects
[SPI]Engineering Sciences [physics] ,[SPI] Engineering Sciences [physics] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2022
7. Altered microRNA expression in severe COVID‐19: Potential prognostic and pathophysiological role.
- Author
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Garnier, Nathalie, Pollet, Kato, Fourcot, Marie, Caplan, Morgan, Marot, Guillemette, Goutay, Julien, Labreuche, Julien, Soncin, Fabrice, Boukherroub, Rabah, Hober, Didier, Szunerits, Sabine, Poissy, Julien, and Engelmann, Ilka
- Subjects
COVID-19 ,CHEMOKINE receptors ,UBIQUITINATION ,SARS-CoV-2 - Abstract
We found that miRNA expression was globally reduced in severe COVID-19 and identified several miRNAs of interest to discriminate severe and non-severe COVID-19. The pathophysiology of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is beginning to be elucidated but the role of microRNAs (miRNAs), small non-coding RNAs that regulate gene expression, remains incompletely understood. In conclusion, our analysis of miRNA expression in nasopharyngeal swabs revealed a general reduction of miRNA expression in severe COVID-19 patients. [Extracted from the article]
- Published
- 2022
- Full Text
- View/download PDF
8. Les clefs de la réussite à Grenoble aux Épreuves classantes nationales : les formations intégrées hospitalo-universitaire
- Author
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Gillois, Pierre, primary, Fourcot, Marie, additional, Genty, Céline, additional, Morand, Patrice, additional, and Bosson, Jean-Luc, additional
- Published
- 2015
- Full Text
- View/download PDF
9. [The keys to success in French Medical National Ranking Examination: Integrated training activities in teaching hospital and medical school].
- Author
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Gillois P, Fourcot M, Genty C, Morand P, and Bosson JL
- Subjects
- Adult, Career Choice, Female, France, Humans, Internship and Residency, Logistic Models, Male, Medicine, Achievement, Curriculum, Education, Medical, Educational Measurement, Hospitals, Teaching, Schools, Medical
- Abstract
Introduction: The National Ranking Examination (NRE) is the key to the choice of career and specialty for future physicians; it lets them choose their place of employment in a specialty and an hospital for their internship. It seems interesting to model the success factors to this exam for the medical students from Grenoble University., Methods: For each of the medical students at Grenoble University who did apply to the NRE in 2012, data have been collected about their academic background and personal details from the administration of the University. A simple logistic regression with success set as being ranked in the first 2000 students, then a polytomous logistic regression, have been performed., Results: The 191 students in the models are 59% female, 25 years old in average (SD 1.8). The factors associated to a ranking in the first 2000 are: not repeating the PCEM1 class (odds ratio [OR] 2.63, CI95: [1.26; 5.56]), performing nurse practice during internships (OR=1.27 [1.00; 1.62]), being ranked in the first half of the class for S3 pole (OR=6.04 [1.21; 30.20] for the first quarter, OR=5.65 [1.15; 27.74] for the second quarter) and being in the first quarter at T5 pole (OR=3.42 [1.08; 10.82])., Conclusion: Our study finds four factors independently contributing to the success at NRE: not repeating PCEM1, performing nurse practice and being ranked in the top of the class at certain academic fields. The AUC is 0.76 and student accuracy is more than 80%. However, some items, for example repeating DCEM4 or participating in NRE mock exams, have no influence on success. A different motivation should be a part of the explanation… As these analysed data are mainly institutional, they are accurate and reliable. The polytomic logistic model, sharing 3 factors with the simple logistic model, replace a performing nurse practice factor's by a grant recipient factor., (Copyright © 2015 Elsevier Masson SAS. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
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