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5. Physical Profile and Impact of a Calcium-Incorporated Implant Surface on Preosteoblastic Cell Morphologic and Differentiation Parameters: A Comparative Analysis.

Author

Lollobrigida, Marco, Lamazza, Luca, Capuano, Cristina, Formisano, Giuseppe, Serra, Emanuele, Laurito, Domenica, Romanelli, Maddalena, Molinari, Agnese, and De Biase, Alberto

Subjects
ALKALINE phosphatase, ANIMAL experimentation, CELL culture, COLORIMETRY, COMPARATIVE studies, ENZYME-linked immunosorbent assay, MICE, SCANNING electron microscopy, STATISTICS, DATA analysis, STATISTICAL reliability, DATA analysis software, DESCRIPTIVE statistics, IN vitro studies, ONE-way analysis of variance
Abstract

Purpose: To assess and compare topographic features and preosteoblastic cell responses of a new hydrothermally treated, calcium-incorporated surface against other commercially available implant surfaces. Materials and Methods: Four different surfaces were the subject of comparison in this study: machined (MC), resorbable blast media (RBM), sandblasted/large-grit/acid-etched (SLA), and calcium-incorporated SLA (Ca-SLA). Surface morphology and roughness were first characterized by scanning electron microscope (SEM) and white light interferometer, respectively. Preosteoblastic MC3T3-E1 cells were then cultured on the titanium surfaces. Cell morphology was observed at 24 hours, 48 hours, 7 days, and 15 days by SEM; differentiation was assessed at 7, 11, and 15 days by assaying alkaline phosphatase (ALP) activity and osteocalcin (OCN) levels. Results: Surface characterization revealed nanotopographic features on Ca-SLA. At topographic analysis, SLA and Ca-SLA showed similar roughness values. Significant differences in cell differentiation parameters were found only at 15 days between the SLA surfaces (both Ca-incorporated and nonincorporated) and MC. Conclusion: Collectively, this study demonstrated that hydrothermal treatment determines the formation of nanotopography without altering the SLA microtopography. Moreover, Ca-SLA and SLA induce MC3T3-E1 cell differentiation at comparable levels. [ABSTRACT FROM AUTHOR]

Published
2016
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8. Primary Retinal Cultures as a Tool for Modeling Diabetic Retinopathy: An Overview

Author

Matteucci, Andrea, Varano, Monica, Mallozzi, Cinzia, Gaddini, Lucia, Villa, Marika, Gabrielli, Sara, Formisano, Giuseppe, Pricci, Flavia, and Malchiodi-Albedi, Fiorella

Subjects
Article Subject
Abstract

Experimental models of diabetic retinopathy (DR) have had a crucial role in the comprehension of the pathophysiology of the disease and the identification of new therapeutic strategies. Most of these studies have been conducted in vivo, in animal models. However, a significant contribution has also been provided by studies on retinal cultures, especially regarding the effects of the potentially toxic components of the diabetic milieu on retinal cell homeostasis, the characterization of the mechanisms on the basis of retinal damage, and the identification of potentially protective molecules. In this review, we highlight the contribution given by primary retinal cultures to the study of DR, focusing on early neuroglial impairment. We also speculate on possible themes into which studies based on retinal cell cultures could provide deeper insight.

Published
2015
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10. The Reverse Transcription Inhibitor Abacavir Shows Anticancer Activity in Prostate Cancer Cell Lines

Author

Carlini, Francesca, primary, Ridolfi, Barbara, additional, Molinari, Agnese, additional, Parisi, Chiara, additional, Bozzuto, Giuseppina, additional, Toccacieli, Laura, additional, Formisano, Giuseppe, additional, De Orsi, Daniela, additional, Paradisi, Silvia, additional, Grober, OlÌ Maria Victoria, additional, Ravo, Maria, additional, Weisz, Alessandro, additional, Arcieri, Romano, additional, Vella, Stefano, additional, and Gaudi, Simona, additional

Published
2010
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22. Biocompatibility assessment of silicone oil and perfluorocarbon liquids used in retinal reattachment surgery in rat retinal cultures

Author

MalchiodiAlbedi, Fiorella, Morgillo, Antonio, Formisano, Giuseppe, Paradisi, Silvia, Perilli, Roberto, Scalzo, Giovanna Carnovale, Scorcia, Giovanni, and Caiazza, Salvatore

Abstract

The effects of silicone oil and perfluorocarbon liquids used in retinal reattachment surgery were studied in vitrousing rat retinal cultures seeded on microporous inserts. These inserts allow the cell layer to be in contact with the material to be tested on the apical side and with the nutrient medium on the basal side. The materials tested were silicone oil, the perfluorocarbons perfluorophenanthrene and perfluoroctane, and hydroxypropylmethylcellulose. Perfluorophenanthrene, the heaviest of the compounds, induced a very precocious detachment of the cell layer. All the other tested biomaterials were compatible with cell survival and did not alter the structural organization of the retinal cultures, as revealed by scanning electron microscopy. By immunocytochemical techniques we evaluated the cell composition and the differentiation state of each of the cultures. In both control and treated samples, neuronal cells were well preserved. The expression of microtubule−asso ciated protein 2, a marker of differentiated neuronal cytoskeleton, was not affected. Amacrine neurons, immunolabeled for γaminobutyric acid, still were detectable after treatment. Synapses, marked by immunoreactivity for synapthophysin, were equally preserved. Vimentinpositive glial cells did not show modifications. The apoptotic rate, as determined by the terminal transferasemediated dUTP–biotin nick endlabeling assay, was similar in treated and control samples. The results confirm that the use of biomaterials with a specific gravity close to intraocular fluids is compatible with retinal cell survival and differentiation in vitro. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 60: 548–555, 2002

Published
2002
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23. Alteration of organized structure of biofilm formed by Staphylococcus epidermidison soft contact lenses

Author

Perilli, Roberto, Marziano, Maria Luisa, Formisano, Giuseppe, Caiazza, Salvatore, Scorcia, Giovanni, and Baldassarri, Lucilla

Abstract

The effect of a nonsteroidal antiinflammatory drug commercially available in eye drop form sodium diclofenac was assayed for its ability to affect biofilms formed by clinical Staphylococcus epidermidisisolates. Biofilms produced by one strain positive for a slimeassociated antigen, suggested to be expressed by more virulent strains, was not affected by sodium diclofenac treatment. On the other hand, biofilm produced by the slimepositive, antigennegative strain showed dramatic alterations already after short treatments with sodium diclofenac as reported for salicylate and other nonsteroidal drugs. Such results suggest further investigation of the possible use of sodium diclofenac drops in the treatment of ophthalmic infections in soft contact lens wearers. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 49, 53–57, 2000.

Published
2000
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25. Segmental transverse colectomy. Minimally invasive versus open approach: results from a multicenter collaborative study

Author

Milone, M., Degiuli, M., Velotti, N., Manigrasso, M., Vertaldi, S., D'Ugo, D., De Palma, G. D., Bruzzese, D., Servillo, G., De Simone, G., Di Lauro, K., Pavanello, Sofia, Ettore Allaix, M., Morino, M., Reddavid, R., Rega, D., Alberto Ammirati, C., Scabini, S., Anania, G., Barberis, A., Longhin, R., Belli, A., Bianco, F., Formisano, G., Giuliani, G., Pietro Bianchi, P., Cavaliere, D., Solaini, L., Coco, C., Rizzo, G., Coratti, A., Tribuzi, A., De Luca, R., Simone, M., Di Leo, A., De Manzoni, G., De Nardi, P., Elmore, U., Rosati, R., Vignali, A., Delrio, P., Pace, U., Di Cataldo, A., Li Destri, G., Donini, A., Graziosi, L., Fontana, A., Mineccia, M., Gentilli, S., Monni, M., Guerrieri, M., Ortenzi, M., Pecchini, F., Piccoli, M., Pedrazzani, C., Turri, G., Pollesel, S., Roviello, F., Rigamonti, M., Zuolo, M., Santarelli, M., Saraceno, F., Sileri, P., Sigismondo Sica, G., Siragusa, L., Pucciarelli, S., Zuin, M., Bombardini, C., Milone, Marco, Degiuli, Maurizio, Velotti, Nunzio, Manigrasso, Michele, Vertaldi, Sara, D'Ugo, Domenico, De Palma, Giovanni Domenico, Dario Bruzzese, Giuseppe Servillo, Giuseppe De Simone, Katia Di Lauro, Silvia Sofia, Marco Ettore Allaix, Mario Morino, Rossella Reddavid, Carlo Alberto Ammirati, Stefano Scabini, Gabriele Anania, Cristina Bombardini, Andrea Barberis, Roberta Longhin, Andrea Belli, Francesco Bianco, Giampaolo Formisano, Giuseppe Giuliani, Paolo Pietro Bianchi, Davide Cavaliere, Leonardo Solaini, Claudio Coco, Gianluca Rizzo, Andrea Coratti, Raffaele De Luca, Michele Simone, Alberto Di Leo, Giovanni De Manzoni, Paola De Nardi, Ugo Elmore, Riccardo Rosati, Andrea Vignali, Paolo Delrio, Ugo Pace, Daniela Rega, Antonio Di Cataldo, Giovanni Li Destri, Annibale Donini, Luigina Graziosi, Andrea Fontana, Michela Mineccia, Sergio Gentilli, Manuela Monni, Mario Guerrieri, Monica Ortenzi, Francesca Pecchini, Micaela Piccoli, Italy. Corrado Pedrazzani, Giulia Turri, Sara Pollesel, Franco Roviello, Marco Rigamonti, Michele Zuolo, Mauro Santarelli, Federica Saraceno, Pierpaolo Sileri Giuseppe Sigismondo Sica, Luigi Siragusa Salvatore Pucciarelli, Matteo Zuin, and Rosati, Riccardo

Subjects
medicine.medical_specialty, Ileus, Nausea, Anemia, Operative Time, Anastomosis, Postoperative Complications, Laparoscopic, Robotic Surgical Procedures, Minimally invasive surgery, Tumor stage, medicine, Transverse Colectomy, Humans, Robotic surgery, Colectomy, Retrospective Studies, Robotic, Transverse colon cancer, Length of Stay, Treatment Outcome, Colonic Neoplasms, Laparoscopy, business.industry, medicine.disease, Surgery, Settore MED/18, medicine.symptom, business
Abstract

The role of minimally invasive surgery in the treatment of transverse colon cancer is still controversial. The aim of this study is to investigate the advantages of a totally laparoscopic technique comparing open versus laparoscopic/robotic approach. Three hundred and eighty-eight patients with transverse colon cancer, treated with a segmental colon resection, were retrospectively analyzed. Demographic data, tumor stage, operative time, intraoperative complications, number of harvested lymph nodes and recovery outcomes were recorded. Recurrences and death were also evaluated during the follow-up. No differences were found between conventional and minimally invasive surgery, both for oncological long-term outcomes (recurrence rate p = 0.28; mortality p = 0.62) and postoperative complications (overall rate p = 0.43; anemia p = 0.78; nausea p = 0.68; infections p = 0.91; bleeding p = 0.62; anastomotic leak p = 0.55; ileus p = 0.75). Nevertheless, recovery outcomes showed statistically significant differences in favor of minimally invasive surgery in terms of time to first flatus (p = 0.001), tolerance to solid diet (p = 0.017), time to first mobilization (p = 0.001) and hospital stay (p = 0.004). Compared with laparoscopic approach, robotic surgery showed significantly better results for time to first flatus (p = 0.001), to first mobilization (p = 0.005) and tolerance to solid diet (p = 0.001). Finally, anastomosis evaluation confirmed the superiority of intracorporeal approach which showed significantly better results for time to first flatus (p = 0.001), to first mobilization (p = 0.003) and tolerance to solid diet (p = 0.001); moreover, we recorded a statistical difference in favor of intracorporeal approach for infection rate (p = 0.04), bleeding (p = 0.001) and anastomotic leak (p = 0.03). Minimally invasive approach is safe and effective as the conventional open surgery, with comparable oncological results but not negligible advantages in terms of recovery outcomes. Moreover, we demonstrated that robotic approach may be considered a valid option and an intracorporeal anastomosis should always be preferred.

Published
2022

26. Verba tene, res sequantur

Author

FASSO', ANDREA, ROBERTO ANTONELLI, CORRADO BOLOGNA, PAOLO CHERCHI, ANDREA FASSÒ, LUCIANO FORMISANO, GIUSEPPE FRASSO, PAOLO MANINCHEDDA, GIUSEPPE MARCI, LAURA SANNIA NOWÉ, NICOLA TANDA, PAOLO MANINCHEDDA, and FASSO' A.

Published
2004

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