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5. 2023 January ESIP Meeting Highlights

Author

Carter, Megan, Shingledecker, Susan, Mills, Allison, Burgess, Annie, Allen, Patty, Breseman, Kelsey, Wyborn, Lesley, lippincott, sarah, Rosales, Colleen Marciel F., Young, Steve, Blodgett, David, Yang, Chaowei, Shepherd, Adam, Duerr, Ruth, Formel, Stephen K., Jones, Dave, Olding, Stephen, McGranaghan, Ryan, Segessenman, Daniel, and Modekurty, Sruti

Abstract

This presentation, originally given during a webinar on February 22, 2023, provides an overview of plenaries and breakout sessions from the 2023 January Earth Science Information Partners (ESIP) Meeting held online from January 23-27, 2023. The meeting theme was Opening Doors to Open Science.

Published
2023
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7. Decay of oil residues in the soil is enhanced by the presence of Spartina alterniflora, with no additional effect from microbiome manipulation

Author

Formel, Stephen K., primary, Martin, Allyson M., additional, Pardue, John H., additional, Elango, Vijaikrishnah, additional, Johnson, Kristina, additional, Gunsch, Claudia K., additional, Lefèvre, Emilie, additional, Varner, Paige M., additional, Kim, Yeon Ji, additional, Bernik, Brittany M., additional, and Van Bael, Sunshine A., additional

Published
2022
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12. Single-Molecule RNA In Situ Hybridization (smFISH) and Immunofluorescence (IF) in the Drosophila Egg Chamber.

Author

Bayer LV, Batish M, Formel SK, and Bratu DP

Subjects
Animals, Drosophila melanogaster genetics, Female, Fluorescent Dyes chemistry, In Situ Hybridization, Fluorescence, Ovum growth & development, RNA genetics, Molecular Biology methods, Oogenesis genetics, Ovum ultrastructure, RNA isolation & purification
Abstract

Detection of nucleic acids in whole tissues has become key in our understanding of gene expression during development. In situ hybridization (ISH) has been an invaluable technique in the making of numerous discoveries. Most recently, the technical advance of using short, fluorescently labeled probes has allowed for the detection of single-mRNA molecules. Thus, quantification of RNA levels in single cells or even within subcellular regions is now possible without RNA isolation. In combination with the immunofluorescence (IF) technique, visualization of nucleic acids and associating proteins is achieved with higher resolution than ever before using light microscopy. Here we describe the steps implemented to achieve the visualization of individual messenger RNAs (mRNA) using single-molecule FISH (smFISH) probes, as well as detection of mRNA/protein (mRNP) complexes via smFISH in combination with IF.

Published
2015
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