Search

Pest ants are known for their damage to biodiversity, harm to agriculture, and negative impact on human welfare. Ants thrive when environmental opportunities arise, becoming pests and/or invading non-native areas. As social insects, they are extremely difficult to control using sustainable methods like biological control. The latter, although safer to the environment, acts slowly allowing the ants to use their individual and social defenses. Among biocontrol agents, fungal pathogens were proposed as promising, however, it is difficult to ascertain their success when the bibliography has not been reviewed and condensed. Therefore, this paper is the first in performing such task by analyzing publications mainly from 2000 to 2022 about the control of pest ants by fungi. From 85 publications selected, 77% corresponded to laboratory studies. Beauveria and Metarhizium were the genera most used in laboratory and field studies. Most of them included Acromyrmex and Atta leaf-cutter ants (LCA), and Solenopsis fire ants. From laboratory experiments, we evaluated how ant net mortality was affected by ant and fungal species, and also by origin, concentration, and inoculation technique of the fungal strains tested. Beauveria bassiana and Metarhizium anisopliae produced the greatest mortality, along with the inoculation spray technique and fungal strains collected from ants. There was a positive relationship between ant mortality and fungal concentration only for those studies which evaluated more than one concentration. Twenty field experimental studies were found, covering 13 pest species, mainly LCA and Solenopsis invicta. Only B. bassiana was tested on Solenopsis, M. anisopliae was mostly used for Acromyrmex, and M. anisopliae or Trichoderma were mainly used with Atta species. The median control field efficiency varied from 20% to 85% for different fungi and ant genera. When grouping all fungal species together, the median control efficiency seemed to be better for Acromyrmex (67%) than for Atta and Solenopsis (both 43%). Our review shows that, at this stage of knowledge, it is very difficult to extrapolate any result. We offer suggestions to improve and standardize laboratory and field experimental studies in order to advance more efficiently in the fungal control of pest ants.

Additive manufacturing (AM) is an attractive set of processes that are being employed lately to process specific materials used in the fabrication of electrical machine components. This is because AM allows for the preservation or enhancement of their magnetic properties, which may be degraded or limited when manufactured using other traditional processes. Soft magnetic materials (SMMs), such as Fe–Si, Fe–Ni, Fe–Co, and soft magnetic composites (SMCs), are suitable materials for electrical machine additive manufacturing components due to their magnetic, thermal, mechanical, and electrical properties. In addition to these, it has been observed in the literature that other alloys, such as soft ferrites, are difficult to process due to their low magnetization and brittleness. However, thanks to additive manufacturing, it is possible to leverage their high electrical resistivity to make them alternative candidates for applications in electrical machine components. It is important to highlight the significant progress in the field of materials science, which has enabled the development of novel materials such as high-entropy alloys (HEAs). These alloys, due to their complex chemical composition, can exhibit soft magnetic properties. The aim of the present work is to provide a critical review of the state-of-the-art SMMs manufactured through different AM technologies. This review covers the influence of these technologies on microstructural changes, mechanical strengths, post-processing, and magnetic parameters such as saturation magnetization (MS), coercivity (HC), remanence (Br), relative permeability (Mr), electrical resistivity (r), and thermal conductivity (k).

Shape memory alloys (SMAs) are a very promising class of metallic materials showing promising nonlinear properties, such as pseudo-elasticity behavior, shape memory effect and damping capacity, due to high mechanical hysteresis and internal friction. SMA have been recently applied in the field of neuromuscular rehabilitation, designing some new devices based on the above properties. The paper discusses possible uses of these materials in the treatment of movement disorders, such as dystonia or hyperkinesia, where their dynamic characteristics can be the key issue.

Las hormigas cortadoras de hojas son una de las plagas más importantes de la región Neotropical. En el presente estudio, fueron recolectados representantes de cuatro provincias de Argentina, con el objetivo de identificar las especies de fóridos que las parasitan. Como resultado, se registraron siete especies de fóridos, Apocephalus neivai Borgmeier, Ap. noetingerorum Disney, Neodohrniphora unichaeta Disney, Myrmosicarius catharinensis Borgmeier, M. cristobalensis Disney, M. crudelis Borgmeier y M. gracilipes Borgmeier, obtenidos en hormigas cortadoras de hojas de las especies Acromyrmex ambiguus Emery, Ac. crassispinus Forel, Ac. heyeri Forel, Ac. lobicornis Emery y Ac. lundii Guérin. Asimismo, son reportados todos los registros previos de hospedadores y su distribución geográfica en Argentina para las siete especies de fóridos. Se citan nuevos registros en todas las especies estudiadas, ampliando así sus rangos geográficos y estableciendo once nuevas interacciones hospedador-parasitoide. Nuestros datos amplían el conocimiento de este poco estudiado sistema y proveen importantes consideraciones para el uso potencial de estos parasitoides como controladores biológicos de hormigas cortadoras de hojas.

Este trabajo tiene como objetivo la identificación morfológica y molecular de cinco especies de Trichoderma asociadas con los nidos de hormigas cortadoras de hojas del género Acromyrmex presentes en Argentina. Las especies identificadas fueron: T. lentiforme, T. inhamatum, T. virens, T. koningiopsis y T. aff. neotropicale resultando éste el primer registro de T. lentiforme y T. inhamatum asociados a hormigas cortadoras de hojas Acromyrmex, en particular Acromyrmex lobicornis y Acromyrmex lundii para el primero y de A. lobicornis para el segundo. Además T. lentiforme representa el primer registro para la Argentina. En este trabajo se amplían medidas del conidióforo y conidios y se aportan datos adicionales sobre la distribución de las especies de Trichoderma en el país. Se ilustran con fotografías los conidióforos, células conidiógenas, conidios y el fenotipo de la colonia para cada especie.

How workers within an ant colony perceive and enforce colony boundaries is a defining biological feature of an ant species. Ants fall along a spectrum of social organizations ranging from single-queen, single nest societies to species with multi-queen societies in which workers exhibit colony-specific, altruistic behaviors towards non-nestmate workers from distant locations. Defining where an ant species falls along this spectrum is critical for understanding its basic ecology. Herein we quantify queen numbers, describe intraspecific aggression, and characterize the distribution of colony sizes for tawny crazy ant (Nylanderia fulva) populations in native range areas in South America as well as in their introduced range in the Southeastern United States. In both ranges, multi-queen nests are common. In the introduced range, aggressive behaviors are absent at all spatial scales tested, indicating that within the population in the Southeastern United States N. fulva is unicolonial. However, this contrasts strongly with intraspecific aggression in its South American native range. In the native range, intraspecific aggression between ants from different nests is common and ritualized. Aggression is typically one-sided and follows a stereotyped sequence of escalating behaviors that stops before actual fighting occurs. Spatial patterns of non-aggressive nest aggregation and the transitivity of non-aggressive interactions demonstrate that results of neutral arena assays usefully delineate colony boundaries. In the native range, both the spatial extent of colonies and the average number of queens encountered per nest differ between sites. This intercontinental comparison presents the first description of intraspecific aggressive behavior for this invasive ant and characterizes the variation in colony organization in the native-range, a pre-requisite to a full understanding of the origins of unicoloniality in its introduced range.

In this paper, the best laser powder bed fusion (L-PBF) printing conditions for FeSi steels with two different Si content (3.0% and 6.5%) are defined. Results show very strict processing window parameters, following a lack of fusion porosity at low specific energy values and keyhole porosity in correspondence with high specific energy values. The obtained microstructure consists of grains with epitaxial growth starting from the grains already solidified in the underling layer. This allows the continuous growth of the columnar grains, directed parallel to the built direction of the component. The magnetic behaviour of FeSi6.5 samples, although the performances found do not still fully reach those of the best commercial electrical steels (used to manufacture magnetic cores of electrical machines and other similar magnetic components), appears to be quite promising. An improvement of the printing process to obtain thin sheets with increased Si content, less than 0.5 mm thick, with accurate geometry and robust structures, can result to an interesting technology for specific application where complex geometries and sophisticated shapes are required, avoiding mechanical machining processes for electrical steel with high silicon content.

The rapidly ascending trend of additive manufacturing techniques requires a tailoring of existing solidification models and the development of new numerical tools. User-friendly numerical models can be a valid aid in order to optimize operating parameter ranges with the scope to extend the modelling tools to already existing or innovative alloys. In this paper a modelling approach is described simulating the generation of single tracks on a powder bed system in a selective laser melting process. The approach we report attains track geometry as a function of: alloy thermo-physical properties, laser speed and power, powder bed thickness. Aim of the research is to generate a numerical tool able to predict laser power and speed ranges in manufacturing porosity-free printed parts without lack of fusion and keyhole pores. The approach is based on a simplified description of the physical aspects. Main simplifications concern: the laser energy input, the formation of the pool cavity, and the powder bed thermo-physical properties. The model has been adjusted based on literature data providing the track’s geometry (width and depth) and relative density. Such data refer to different alloys. In particular, Ti6Al4V, Inconel625, Al7050, 316L and pure copper are considered. We show that the printing process presents features common to all alloys. This allows the model to predict the printing behavior of an alloy from its physical properties, avoiding the need to perform specific experimental activities.

RETRACTION NOTICE for the paper: About some issues concerning shape memory alloys application s in neuro-rehabilitation, METABK 59(1) (2020), 137-140.

Attine ants are a monophyletic group comprising more than 230 species, distributed exclusively in the New World. All higher Attini ants depend on the cultivation of fungus gardens for food, and those gardens are continuously exposed to alien microorganisms. The present work describes, for the first time, the composition, relative frequency and the richness of the fungal species comprising the mycobiota from the garden of the most abundant Acromyrmex species from different regions of Argentina. We also compared, using a multivariate approach, the mycobiota composition with the purpose of testing two hypotheses: 1) whether mycobiota is defined by the ant species, or 2) whether mycobiota is determined by the geographical region. We found that each fungal community had a particular composition and abundance: the species that were highly frequent in a community or site, could be rare or altogether absent in another community or site. Species richness, as well as the theoretical maximum richness, also changed with locations. Furthermore, we found that different ant species at the same site had similar mycobiotas, whereas the same ant species in distant locations had only a few fungal species in common. Therefore, we concluded that mycobiota composition changed considerably with geographical site and was not dependent on the ant species. Our results provide an additional understanding of the leaf cutting-ants system, confirming that the garden of leaf cutting ants nests is a complex and dynamic fungal community which is dependent on the site where it was located. DOI: https://doi.org/10.25260/EA.17.27.3.0.488

In this paper, the capability of laser powder bed fusion (L-PBF) systems to process stainless steel alloys is reviewed. Several classes of stainless steels are analyzed (i.e., austenitic, martensitic, precipitation hardening and duplex), showing the possibility of satisfactorily processing this class of materials and suggesting an enlargement of the list of alloys that can be manufactured, targeting different applications. In particular, it is reported that stainless steel alloys can be satisfactorily processed, and their mechanical performances allow them to be put into service. Porosities inside manufactured components are extremely low, and are comparable to conventionally processed materials. Mechanical performances are even higher than standard requirements. Micro surface roughness typical of the as-built material can act as a crack initiator, reducing the strength in both quasi-static and dynamic conditions.

Pseudacteon flies, parasitoids of worker ants, are being intensively studied as potentially effective agents in the biological control of the invasive pest fire ant genus Solenopsis (Hymenoptera: Formicidae). This is the first attempt to describe the karyotype of P. curvatus Borgmeier, P. nocens Borgmeier and P. tricuspis Borgmeier. The three species possess 2n = 6; chromosomes I and II were metacentric in the three species, but chromosome pair III was subtelocentric in P. curvatus and P. tricuspis, and telocentric in P. nocens. All three species possess a C positive band in chromosome II, lack C positive heterochromatin on chromosome I, and are mostly differentiated with respect to chromosome III. P. curvatus and P. tricuspis possess a C positive band, but at different locations, whereas this band is absent in P. nocens. Heterochromatic bands are neither AT nor GC rich as revealed by fluorescent banding. In situ hybridization with an 18S rDNA probe revealed a signal on chromosome II in a similar location to the C positive band in the three species. The apparent lack of morphologically distinct sex chromosomes is consistent with proposals of environmental sex determination in the genus. Small differences detected in chromosome length and morphology suggests that chromosomes have been highly conserved during the evolutionary radiation of Pseudacteon. Possible mechanisms of karyotype evolution in the three species are suggested.

RESUMEN. Fóridos del género Pseudacteon Coquillett oviponen en forrajeras del género Solenopsis Westwood y empupan en sus cabezas. Se evaluó la relación entre la viabilidad de los parasitoides, la presencia de cuernos respiratorios y el color en los opérculos de los puparios de cuatro especies de Pseudacteon criados sobre Solenopsis invicta Buren y Solenopsis richteri Forel. La presencia de cuernos respiratorios estuvo asociada a la viabilidad de las pupas para las especies consideradas (p < 0,01), pero no el color de los opérculos (p > 0,09); excepto cuando Pseudacteon cultellatus Borgmeier se desarrolló sobre hormigas de la reserva y niñeras de S. invicta (p < 0,01). Los opérculos claros predominaron en P. cultellatus; mientras que en las demás especies, que atacan a hormigas más grandes, predominaron los opérculos castaños. Debido a que los opérculos presentaron un color similar al de la hormiga parasitada, esto representaría un mimetismo por parte del parasitoide para evitar ser detectado por el huésped. Dado que los cuernos respiratorios son necesarios para la supervivencia de la mayoría de las pupas, sugerimos que sean usados como indicadores de eficiencia en los protocolos de cría. Recomendamos usar solo forrajeras dado que las otras castas no parecen ser huéspedes apropiados.ABSTRACT. Pseudacteon Coquillett phorid flies oviposit on Solenopsis Westwood ants and pupate within the ant's head. We have evaluated the relationship between pupae's viability, presence of respiratory horns and the operculum color in four species of Pseudacteon reared on Solenopsis invicta Buren and Solenopsis richteri Forel ants. The presence of respiratory horns was significantly associated with pupae's viability for all species considered (p < 0,001) but operculum color was not (p > 0,09), except (p < 0,01) when Pseudacteon cultellatus Borgmeier had developed on S. invicta nanitic and reserve workers. Pupae with light-colored opercula were more frequent in P. cultellatus, whereas brown opercula were more frequent for the other species that attack bigger ants. Mimetism can be invoked to explain the similarity in opercula color with that of the head of the parasitized ant as a way to avoid recognition by members of the colony. We conclude that the presence of respiratory horns is necessary for pupae survival of most of the pupae and we suggest to use the presence of respiratory horns as an indicator of the efficiency of rearing protocols for this group of parasitoids. We also recommend using forager ants because other casts do not seem to be appropriate hosts.

This review updates and summarizes the current knowledge about the interaction of leaf-cutter ants and their parasitoids by providing comparable data for Acromyrmex and Atta ants. First, an overview of the relevant aspects of the biology and taxonomy of leaf cutters and of their parasitoids is provided. Second, I show the peculiarities of the parasitoids attacking behaviors towards their host as well as the responses or ant defenses against the phorids exhibited by their hosts. Third, I discuss relevant aspects of the interactions between hosts and parasitoids. Finally, the review ends demonstrating why these phorids could be promising biological control agents of leaf-cutter pests and suggests priority lines of research for the future.

Las hormigas son importantes fuentes de perturbación en el suelo al generar cambios físicos y químicos que pueden afectar a los microorganismos. Camponotus punctulatus, una hormiga nativa de Argentina, construye nidos de gran tamaño en situaciones de perturbación agrícola. Estos hormigueros presentan una mayor concentración de nutrientes en comparación con el suelo control. Determinamos si la alta concentración de nutrientes puede deberse a un aumento de la descomposición y mineralización, producto de la mayor abundancia y actividad de los microorganismos en el hormiguero. Además, documentamos las variaciones estacionales de la biomasa y actividad microbiana, con la hipótesis de mayor biomasa y actividad microbiana en las épocas del año con mayor temperatura y humedad. Colectamos seis muestras pareadas (en el hormiguero y afuera de éste) en cinco épocas diferentes a lo largo de un año, en una pastura de Setaria sphacelata en Corrientes, Argentina. En cada muestreo enterramos bolsas de descomposición (1 μm de poro) con Setaria sphacelata y retiramos las enterradas en el muestreo anterior. Se cuantificó la biomasa microbiana, la actividad microbiana (deshidrogenasa total y fúngica) y la actividad celulasa de las muestras de suelo, y la descomposición microbiana de las bolsas de descomposición. Un Análisis de Componentes Principales indicó que los hormigueros se caracterizaban por una menor actividad deshidrogenasa durante todas las épocas muestreadas en comparación con el suelo alejado de su influencia. Sin embargo, la descomposición fue significativamente más alta en el hormiguero en los períodos enero-abril y abril-julio. La comparación de la actividad deshidrogenasa total y la fúngica mostró que había una mayor proporción de actividad bacteriana en los hormigueros. La biomasa y la actividad de los microorganismos, así como la descomposición, variaron estacionalmente. El pico de actividad microbiana fue en enero, mientras que se registró mayor biomasa microbiana durante los meses de invierno. La descomposición se correlacionó positivamente con la actividad deshidrogenasa y celulasa, pero negativamente con la biomasa microbiana en ambos micrositios. Nuestros resultados sugieren que la biomasa microbiana refleja principalmente los microorganismos inactivos (ya que se correlacionó negativamente con la descomposición), mientras que la actividad deshidrogenasa se propone como un buen indicador del estado de los microorganismos del suelo. La mayor proporción de la actividad bacteriana en el hormiguero podría contribuir a explicar el aumento de los nutrientes en ese micrositio (entre otras posibilidades que se discuten); sin embargo, son necesarios más estudios al respecto.

Agricultural activity in NE Argentina promotes the demographic explosion of the ant Camponotus punctulatus Mayr. We compared ant activity between a natural grassland and a neighbouring sown pasture in north-eastern Argentina during 24 h periods during three different times in the year. We offered sugar and tuna baits to ants in order to assess ant foraging activity. We set pitfalls during the summer to quantify ant activity independently of their responses to food resources. We also used bait and pitfall data to estimate ant diversity. C. punctulatus was the single ant species present at baits in the sown pasture. Significantly greater numbers of C. punctulatus in comparison to other ants were found at pitfalls at the agriculturally-disturbed plot when compared with the natural grassland, although no differences in ant species richness were observed between plots. C. punctulatus was active throughout the day. Foraging of the ant community differed significantly between the sown pasture and the natural grassland. Foraging activity of C. punctulatus and all other ant species exhibited opposite patterns in the natural grassland. The successful establishment of C. punctulatus after agricultural disturbance seemed to have a negative effect on the foraging patterns of the ant community through a change in the relative abundance of ant species.

In order to gain insight about why Patagonia has low levels of granivory activity, percentages of world-wide granivory rates were compared for rodents, ants, and birds in seven desertic areas of the world. Using a series of multivariate analyses, we classified and ordinated these areas according to percentages of granivory rates, environmental features of the sites, and granivore biodiversity. Granivory activity was clearly separated into two groups, one representing the Northern Hemisphere which comprised Sonora, Great Basin, and Israel, and another, representing the Southern Hemisphere with Monte, Patagonia, South Africa, and Australia. The ordination analyses did not discrimínate any clear groups using the combined environmental variables. Separate correlations between the ordination axes of granivory and each environmental variable, and granivore richness, showed that only thermal range (the difference between the extreme annual mean temperatures) successfully correlated with the differences ín overall granivory between deserts and hemispheres. Our results show that all sites from the Northern Hemisphere, which had a very high continentality (high land/ocean ratio), and therefore high thermal range, were the ones with greater levels of granivory. A linear regression analysis showed that 72 % of the variation in overall granivory, mainly driven by rodent activity, was explained by thermal range. We propose that the only strategy that can evolve in environments with high thermal range ís granivory, as seeds are the only high quality food that can be stored. We propose that the combination of strong selective pressures with the chance that a certain taxa has for being at a certain place and time determines the relative importance of different taxa as granivores. Murid rodents had arrived earlier in the Northern Hemisphere than ín the Southern, and therefore, had greater opportunity to develop the granivore syndrome. Ants -which are as old as rodents at some of the studied sites but are poikilotherms- cannot deal as efficiently as rodents with very harsh environments. Birds, finally, avoid bad situations by migrating to more favourable habitats, and therefore circumvent those selective pressures with their great vagility. The more beningn the environment (lower thermal range due to low land/ocean ratios, as in the Southern Hemisphere), the less selective pressure for granivory, a reason that can also account for the high number of omnivores in South America and Australia, and the low granivory rates in Patagonia.

The diet of the leaf-cutter ant Acromyrmex striatus was used as indirect evidence for potential antiherbivore activity by the nectivorous ant Camponotus blandus and by chemical and mechanical plant defenses. Data on ant activity, plant abundance, and foliar material for analyses of chemical and mechanical plant defenses were collected during spring in a subtropical forest in the Argentinean Chaco. We found a negative relationship between the proportion of visits by C. blandus and leaf harvesting of A. striatus for the plant species that offer nectar. However, the most abundant plant species in the forest comprised the greatest part of the leaf-cutter diet. In general, no relationship was found between chemical or mechanical defenses and leaf-cutter diet for all the plant species of the forest. A few plant species with high levels of plant defenses suffered little or no harvesting by leaf-cutter ants. Our findings suggest two main reasons for the absence or low representation of nectar-offering plant species in the diet of A. striatus: nectivorous ant activity and low plant species abundances.

Light acclimation in gas exchange properties was found in three different Neotropical species of Cecropia under greenhouse conditions. Two species are habitat specialists (C. tessmannii, a shade species and C. membranacea, a sun species), and the third ís a generalist (C. pachystachya). The shade species showed the least flexibility. Rates of photosynthesis were positively correlated with light intensify, transpiration and conductance. Photosynthetic rates for each species ranked in the same order as growth rates. The generalist species, C. pachystachya performed, oil average, better than any of the specialists in their own light environment.