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2. The urinary excretion of epigenetically modified DNA as a marker of pediatric ALL status and chemotherapy response

Author

Rozalski, Rafal, Gackowski, Daniel, Skalska-Bugala, Aleksandra, Starczak, Marta, Siomek-Gorecka, Agnieszka, Zarakowska, Ewelina, Modrzejewska, Martyna, Dziaman, Tomasz, Szpila, Anna, Linowiecka, Kinga, Guz, Jolanta, Szpotan, Justyna, Gawronski, Maciej, Labejszo, Anna, Gackowska, Lidia, Foksinski, Marek, Olinska, Elwira, Wasilow, Aleksandra, Koltan, Andrzej, Styczynski, Jan, and Olinski, Ryszard

Published
2021
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6. Unprecedentedly High Level of Intracellular Vitamin C and DNA Epigenetic Marks in Prostate: Relevant for Male Fertility?

Author

Guz, Jolanta, Zarakowska, Ewelina, Mijewski, Pawel, Wasilow, Aleksandra, Szpotan, Justyna, Foksinski, Marek, Brzoszczyk, Bartosz, Gackowski, Daniel, Jarzemski, Piotr, and Olinski, Ryszard

Subjects
VITAMIN C, LIQUID chromatography-mass spectrometry, SEMEN, PROSTATE, BENIGN prostatic hyperplasia, FREE radical scavengers, DNA demethylation, FERTILITY
Abstract

Background/Aims: Seminal plasma composition is affected by the physiological state of the prostate, the major male reproductive gland. Semen components, like vitamin C, can modulate sperm function. Vitamin C is an effective scavenger of free radicals and is an essential component of enzymes such as TET proteins involved in the DNA demethylation process. In the present study, a broad range of parameters which may influence the metabolic state of the prostate gland were analysed including blood and prostate tissue vitamin C, epigenetic DNA modifications and 8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA of leukocytes and prostate tissues. Methods: The experimental material were tissue samples from patients with benign prostatic hyperplasia (BPH), normal/marginal prostate tissues from prostate cancer patients, leukocytes from healthy donors, and blood plasma from BPH patients and healthy donors. We applied ultra-performance liquid chromatography methods with mass spectrometry and/or UV detection. Results: We found an unprecedentedly high level of intracellular vitamin C in all analysed prostatic tissues (benign prostatic hyperplasia and normal, marginal ones), a value much higher than in leukocytes and most human tissues. DNA epigenetic patterns in prostate cells are similar to other soft tissues like the colon, however, its uniqueness is the unprecedentedly high level of 5-(hydroxymethyl)-2'-deoxyuridine and a significant increase in 5-formyl-2'-deoxycytidine value compared to aforementioned tissues. Moreover, the level of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an established marker of oxidative stress, is significantly higher in prostate tissues than in leukocytes and many previously studied soft tissues. Conclusion: Our results pointed out that prostatic vitamin C (regarded as the main supplier of the vitamin C to seminal plasma) and the DNA modifications (which may be linked to the regeneration of prostate epithelium) may play important role to maintain the prostate health. [ABSTRACT FROM AUTHOR]

Published
2023
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7. In vivo evidence of ascorbate involvement in the generation of epigenetic DNA modifications in leukocytes from patients with colorectal carcinoma, benign adenoma and inflammatory bowel disease

Author

Starczak, Marta, Zarakowska, Ewelina, Modrzejewska, Martyna, Dziaman, Tomasz, Szpila, Anna, Linowiecka, Kinga, Guz, Jolanta, Szpotan, Justyna, Gawronski, Maciej, Labejszo, Anna, Liebert, Ariel, Banaszkiewicz, Zbigniew, Klopocka, Maria, Foksinski, Marek, Gackowski, Daniel, and Olinski, Ryszard

Published
2018
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8. Characteristic profiles of DNA epigenetic modifications in colon cancer and its predisposing conditions—benign adenomas and inflammatory bowel disease

Author

Dziaman, Tomasz, Gackowski, Daniel, Guz, Jolanta, Linowiecka, Kinga, Bodnar, Magdalena, Starczak, Marta, Zarakowska, Ewelina, Modrzejewska, Martyna, Szpila, Anna, Szpotan, Justyna, Gawronski, Maciej, Labejszo, Anna, Liebert, Ariel, Banaszkiewicz, Zbigniew, Klopocka, Maria, Foksinski, Marek, Marszalek, Andrzej, and Olinski, Ryszard

Published
2018
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10. Epigenetic DNA changes in childhood acute lymphoblastic leukemia - the drug treatment effect.

Author

Rozalski, Rafal, primary, Gackowski, Daniel, additional, Skalska, Aleksandra, additional, Starczak, Marta, additional, Siomek-Gorecka, Agnieszka Siomek-Gorecka, additional, Zarakowska, Ewelina, additional, Modrzejewska, Martyna, additional, Dziaman, Tomasz, additional, Szpila, Anna, additional, Linowiecka, Kinga, additional, Guz, Jolanta, additional, Szpotan, Justyna, additional, Gawronski, Maciej, additional, Labejszo, Anna, additional, Gackowska, Lidia, additional, Foksinski, Marek, additional, Olinska, Elwira, additional, Wasilow, Aleksandra, additional, Koltan, Andrzej, additional, Styczynski, Jan, additional, and Olinski, Ryszard, additional

Published
2021
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18. MOESM1 of In vivo evidence of ascorbate involvement in the generation of epigenetic DNA modifications in leukocytes from patients with colorectal carcinoma, benign adenoma and inflammatory bowel disease

Author

Starczak, Marta, Zarakowska, Ewelina, Modrzejewska, Martyna, Dziaman, Tomasz, Szpila, Anna, Linowiecka, Kinga, Guz, Jolanta, Szpotan, Justyna, Gawronski, Maciej, Labejszo, Anna, Liebert, Ariel, Banaszkiewicz, Zbigniew, Klopocka, Maria, Foksinski, Marek, Gackowski, Daniel, and Olinski, Ryszard

Abstract

Additional file 1. Tables S1â S3, Figures S1â S10, additional method.

Published
2018
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19. Additional file 3: of Characteristic profiles of DNA epigenetic modifications in colon cancer and its predisposing conditionsâ benign adenomas and inflammatory bowel disease

Author

Dziaman, Tomasz, Gackowski, Daniel, Guz, Jolanta, Linowiecka, Kinga, Bodnar, Magdalena, Starczak, Marta, Zarakowska, Ewelina, Modrzejewska, Martyna, Szpila, Anna, Szpotan, Justyna, Gawronski, Maciej, Labejszo, Anna, Liebert, Ariel, Banaszkiewicz, Zbigniew, Klopocka, Maria, Foksinski, Marek, Marszalek, Andrzej, and Olinski, Ryszard

Subjects
body regions, nervous system, fungi, biochemical phenomena, metabolism, and nutrition
Abstract

Table S3. Primers and short hydrolysis probes used for TETs and AID mRNA expression analysis. (PDF 442Â kb)

Published
2018
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20. Additional file 2: of Characteristic profiles of DNA epigenetic modifications in colon cancer and its predisposing conditionsâ benign adenomas and inflammatory bowel disease

Author

Dziaman, Tomasz, Gackowski, Daniel, Guz, Jolanta, Linowiecka, Kinga, Bodnar, Magdalena, Starczak, Marta, Zarakowska, Ewelina, Modrzejewska, Martyna, Szpila, Anna, Szpotan, Justyna, Gawronski, Maciej, Labejszo, Anna, Liebert, Ariel, Banaszkiewicz, Zbigniew, Klopocka, Maria, Foksinski, Marek, Marszalek, Andrzej, and Olinski, Ryszard

Abstract

Table S2. Transition patterns specific detector settings and sources of standards for analyzed compounds (relative response ratio = area under the peak of qualifierion/area under the peak of quantifier ion). (PDF 82Â kb)

Published
2018
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21. Additional file 1: of Characteristic profiles of DNA epigenetic modifications in colon cancer and its predisposing conditionsâ benign adenomas and inflammatory bowel disease

Author

Dziaman, Tomasz, Gackowski, Daniel, Guz, Jolanta, Linowiecka, Kinga, Bodnar, Magdalena, Starczak, Marta, Zarakowska, Ewelina, Modrzejewska, Martyna, Szpila, Anna, Szpotan, Justyna, Gawronski, Maciej, Labejszo, Anna, Liebert, Ariel, Banaszkiewicz, Zbigniew, Klopocka, Maria, Foksinski, Marek, Marszalek, Andrzej, and Olinski, Ryszard

Subjects
body regions, nervous system, fungi
Abstract

Table S1. Characteristics of antibodies used in immunohistochemical staining. (PDF 499Â kb)

Published
2018
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24. Correction: Profiles of a broad spectrum of epigenetic DNA modifications in normal and malignant human cell lines: Proliferation rate is not the major factor responsible for the 5-hydroxymethyl-2′-deoxycytidine level in cultured cancerous cell lines

Author

Foksinski, Marek, primary, Zarakowska, Ewelina, additional, Gackowski, Daniel, additional, Skonieczna, Magdalena, additional, Gajda, Karolina, additional, Hudy, Dorota, additional, Szpila, Anna, additional, Bialkowski, Karol, additional, Starczak, Marta, additional, Labejszo, Anna, additional, Czyz, Jaroslaw, additional, Rzeszowska-Wolny, Joanna, additional, and Olinski, Ryszard, additional

Published
2018
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25. Oxidation Products of 5-Methylcytosine are Decreased in Senescent Cells and Tissues of Progeroid Mice

Author

Zarakowska, Ewelina, primary, Czerwinska, Jolanta, additional, Tupalska, Agnieszka, additional, Yousefzadeh, Matt J, additional, Gregg, Siobhán Q, additional, Croix, Claudette M St, additional, Niedernhofer, Laura J, additional, Foksinski, Marek, additional, Gackowski, Daniel, additional, Szpila, Anna, additional, Starczak, Marta, additional, Tudek, Barbara, additional, and Olinski, Ryszard, additional

Published
2018
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27. The impact of DIDS-induced inhibition of voltage-dependent anion channels (VDAC) on cellular response of lymphoblastoid cells to ionizing radiation.

Author

Skonieczna, Magdalena, Cieslar-Pobuda, Artur, Saenko, Yuriy, Foksinski, Marek, Olinski, Ryszard, Rzeszowska-Wolny, Joanna, Wiechec, Emilia, Skonieczna, Magdalena, Cieslar-Pobuda, Artur, Saenko, Yuriy, Foksinski, Marek, Olinski, Ryszard, Rzeszowska-Wolny, Joanna, and Wiechec, Emilia

Abstract

Background: The voltage-dependent ion channels (VDAC) play an essential role in the cross talk between mitochondria and the rest of the cell. Their implication in cell life and cell death has been studied extensively in recent years. In this work we studied the impact of mitochondrial membrane voltage-dependent anion channels (VDACs) on cell survival and response to X-ionizing radiation (IR) of human lymphoblastoid K562 cells. Methods: The inhibition of VDACs was achieved by 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) inhibitor and in vitro experiments including clonogenity assay, UV-visible spectrophotometry, comet assay and FACS analysis were implemented. Results: Inhibition of VDAC led to augmentation of IR-induced apoptosis and ROS production. Additionally, DIDS affected repair of IR-induced DNA strand breaks and was in line with both induction of apoptosis and caspase activity. The IR-induced NO production was potently reduced by inhibition of VDAC. Conclusion: Our results suggest that VDAC control cellular response to ionizing radiation through modulation of the ROS- and NO-dependent signaling pathways. Inhibition of VDAC with DIDS induced apoptosis in irradiated K562 lymphoblastoid cells points at DIDS, as a promising agent to enhance the effectiveness of radiotherapy., Funding agencies: National Science Center [DEC-2012/07/B/NZ1/00008, NCN 2015/19/B/ST7/02984]; Innovative Economy Operational Programme (POIG) [02.01.00-00-166/08, POIG. 02.03.01-00040/13]

Published
2017
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28. Profiles of a broad spectrum of epigenetic DNA modifications in normal and malignant human cell lines: Proliferation rate is not the major factor responsible for the 5-hydroxymethyl-2′-deoxycytidine level in cultured cancerous cell lines

Author

Foksinski, Marek, primary, Zarakowska, Ewelina, additional, Gackowski, Daniel, additional, Skonieczna, Magdalena, additional, Gajda, Karolina, additional, Hudy, Dorota, additional, Szpila, Anna, additional, Bialkowski, Karol, additional, Starczak, Marta, additional, Labejszo, Anna, additional, Czyz, Jaroslaw, additional, Rzeszowska-Wolny, Joanna, additional, and Olinski, Ryszard, additional

Published
2017
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31. Distinct pattern of epigenetic DNA modification in leukocytes from patients with colorectal carcinoma and individuals with precancerous conditions, benign adenoma and inflammatory bowel disease – a link to oxidative stress

Author

Starczak, Marta, primary, Zarakowska, Ewelina, additional, Modrzejewska, Martyna, additional, Dziaman, Tomasz, additional, Szpila, Anna, additional, Linowiecka, Kinga, additional, Guz, Jolanta, additional, Szpotan, Justyna, additional, Gawronski, Maciej, additional, Labejszo, Anna, additional, Liebert, Ariel, additional, Banaszkiewicz, Zbigniew, additional, Klopocka, Maria, additional, Foksinski, Marek, additional, Gackowski, Daniel, additional, and Olinski, Ryszard, additional

Published
2017
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33. 8-Oxo-7,8-dihydroguanine and uric acid as efficient predictors of survival in colon cancer patients

Author

Dziaman, Tomasz, Banaszkiewicz, Zbigniew, Roszkowski, Krzysztof, Gackowski, Daniel, Wisniewska, Ewa, Rozalski, Rafał, Foksinski, Marek, Siomek, Agnieszka, Speina, Elzbieta, Winczura, Alicja, Marszalek, Andrzej, Tudek, Barbara, and Olinski, Ryszard

Subjects
Biological markers, Survival, Oxidative stress, DNA damage, DNA repair, 8-oxoGua, Uric acid, Colon cancer
Abstract

The aim of this work was to answer the question whether the broad range of parameters which describe oxidative stress and oxidatively damaged DNA and repair are appropriate prognosis factors of colon cancer (CRC) patients survival? The following parameters were analyzed for 89 CRC patients: concentration of uric acid and vitamins A, E, C in plasma; levels of 8-oxodGuo (8-oxo-7,8-dihydro-2'-deoxyguanosine) in DNA of leukocyte and colon tissues; urinary excretion rates of 8-oxodGuo and 8-oxoGua (8-oxo-7,8-dihydroguanine); the activity and mRNA or protein level of repair enzymes OGG1, APE1, ANPG, TDG and PARP1. All DNA modifications and plasma antioxidants were analyzed using high performance liquid chromatography (HPLC) or HPLC/gas chromatography-mass spectrometry techniques. Expression of repair proteins was analyzed by QPCR, Western or immunohistochemistry methods. Longer survival coincided with low levels of 8-oxodGuo/8oxoGua in urine and 8-oxodGuo in DNA as well as with high concentration of uric acid plasma level. In contrast to expectations, longer survival coincided with lower mRNA level in normal colon tissue of the main 8-oxoGua DNA glycosylase, OGG1, but no association was found for PARP-1 expression. When analyzing simultaneously two parameters the discriminating power increased significantly. Combination of low level of urinary 8-oxoGua together with low level of 8-oxodGuo in leukocyte (both below median value) or high concentration of plasma uric acid (above median value) have the best prediction power. Since prediction value of these parameters seems to be comparable to conventional staging procedure, they could possibly be used as markers to predict clinical success in CRC treatment. The authors of this article (R.O., T.D., R.R., D.G., A.S., M.F.) are partners of ECNIS and ECNIS2 (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a Network of Excellence operating within the European Union 6th and 7th Framework Program (contract numbers: 513943 and 266198, respectively).

Published
2013

34. Establishing the background level of base oxidation in human lymphocyte DNA: results of an interlaboratory validation study

Author

Gedik, Catherine, Collins, Andrew, Dubois, Jacques, Duez, Pierre, Kouegnigan, Léonard, Rees, Jean-François, Loft, Steffen, Möller, Peter, Jensen, Annie, Poulsen, Henrik, Riis, Bente, Weimann, Allan, Cadet, Jean, Douki, Thierry, Ravanat, Jean-Luc, Sauvaigo, Sylvie, Faure, Henri, Morel, Isabelle, Morin, Bénédicte, Epe, Bernd, Eckert, Inge, Hartwig, Andrea, Schwerdtle, Tanja, Dolara, Piero, Giovannelli, Lisa, Lodovici, Maura, Guglielmi, Francesco, Olinski, Ryszard, Bialkowski, Karol, Foksinski, Marek, Gackowski, Daniel, Duračková, Zdena, Muchová, Jana, Korytar, Peter, Sivonová, Monika, Dusinska, Maria, Mislanova, Csilla, Petrovska, Helena, Smolkova, Bozena, Vina, José, Lloret, Ana, Saez, Guillermo, Hofer, Tim, Möller, Lennart, Eriksson, Hanna, Gremaud, Eric, Herbert, Karl, Wild, Chris, Kelly, Frank, Dunster, Christina, White, Ann, Wood, Sharon, Vaughan, Nicolas, Department of Computer Science [Liverpool], University of Liverpool, France, Amériques, Espagne – Sociétés, pouvoirs, acteurs (FRAMESPA), Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université libre de Bruxelles (ULB), Université Catholique de Louvain = Catholic University of Louvain (UCL), Universität Ulm - Ulm University [Ulm, Allemagne], Laboratoire Lésions des Acides Nucléiques (LAN), Service de Chimie Inorganique et Biologique (SCIB - UMR E3), Institut Nanosciences et Cryogénie (INAC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut Nanosciences et Cryogénie (INAC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS), Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie (CIBEST ), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), CHU Grenoble, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Université de Rennes (UR), Université de Bordeaux (UB), Department of Preclinical and Clinical Pharmacology, Università degli Studi di Firenze = University of Florence (UniFI), Norwegian Institute for Air Research (NILU), Interface Physique et Chimie pour le Vivant (IPCV), Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Université Toulouse - Jean Jaurès (UT2J)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UNIV-RENNES), Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), and Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Guanine, Analytical chemistry, Biology, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Cell Line, Tumor, Genetics, Humans, AP site, Lymphocytes, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Chromatography, 8-Hydroxy-2'-deoxyguanosine, Deoxyguanosine, DNA oxidation, Formamidopyrimidine DNA glycosylase, DNA, Orders of magnitude (mass), Comet assay, chemistry, 8-Hydroxy-2'-Deoxyguanosine, 030220 oncology & carcinogenesis, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Laboratories, Oxidation-Reduction, Biotechnology, HeLa Cells
Abstract

Accurate measurement of low levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in DNA is hampered by the ease with which guanine is oxidized during preparation of DNA for analysis. ESCODD, a consortium of mainly European laboratories, has attempted to minimize this artifact and to provide standard, reliable protocols for sample preparation and analysis. ESCODD has now analyzed 8-oxodGuo in the DNA of lymphocytes isolated from venous blood from healthy young male volunteers in several European countries. Two approaches were used. Analysis of 8-oxodGuo by HPLC with electrochemical detection was performed on lymphocytes from 10 groups of volunteers, in eight countries. The alternative enzymic approach was based on digestion of DNA with formamidopyrimidine DNA glycosylase (FPG) to convert 8-oxo-7,8-dihydroguanine (8-oxoGua) to apurinic sites, subsequently measured as DNA breaks using the comet assay (7 groups of volunteers, in six countries). The median concentration of 8-oxodGuo in lymphocyte DNA, calculated from the mean values of each group of subjects as determined by HPLC, was 4.24 per 10(6) guanines. The median concentration of FPG-sensitive sites, measured with the comet assay, was 0.34 per 10(6) guanines. Identical samples of HeLa cells were supplied to all participants as a reference standard. The median values for 8-oxodGuo in HeLa cells were 2.78 per 10(6) guanines (by HPLC) and 0.50 per 10(6) guanines (by enzymic methods). The discrepancy between chromatographic and FPG-based approaches may reflect overestimation by HPLC (if spurious oxidation is still not completely controlled) or underestimation by the enzymic method. Meanwhile, it is clear that the true background level of base oxidation in DNA is orders of magnitude lower than has often been claimed in the past.

Published
2004
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37. 8-Oxo-7,8-dihydroguanine and uric acid as efficient predictors of survival in colon cancer patients

Author

Dziaman, Tomasz, primary, Banaszkiewicz, Zbigniew, additional, Roszkowski, Krzysztof, additional, Gackowski, Daniel, additional, Wisniewska, Ewa, additional, Rozalski, Rafał, additional, Foksinski, Marek, additional, Siomek, Agnieszka, additional, Speina, Elzbieta, additional, Winczura, Alicja, additional, Marszalek, Andrzej, additional, Tudek, Barbara, additional, and Olinski, Ryszard, additional

Published
2013
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38. Does morphology of carotid plaque depend on patient's oxidative stress?

Author

Rozalski, Rafal, primary, Migdalski, Arkadiusz, additional, Gackowski, Daniel, additional, Guz, Jolanta, additional, Siomek, Agnieszka, additional, Foksinski, Marek, additional, Szpila, Anna, additional, Zarakowska, Ewelina, additional, Majer, Marcin, additional, Jawien, Arkadiusz, additional, and Olinski, Ryszard, additional

Published
2013
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40. Measurement of DNA oxidation in human cells by chromatographic and enzymic methods

Author

Collins, Andrew, Gedik, Catherine, Vaughan, Nicholas, Wood, Sharon, White, Ann, Dubois, Jacques, Rees, Jean-François, Loft, Steffen, Møller, Peter, Poulsen, Henrik, Cadet, Jean, Douki, Thierry, Ravanat, Jean-Luc, Sauvaigo, Sylvie, Faure, Henry, Morel, Isabelle, Morin, Bénédicte, Epe, Bernd, Phoa, Nicole, Hartwig, Andrea, Schwerdtle, Tanja, Dolara, Piero, Giovannelli, Lisa, Lodovici, Maura, Olinski, Ryszard, Bialkowski, Karol, Foksinski, Marek, Gackowski, Daniel, Duracková, Zdena, Hlinciková, Lucia, Korytar, Peter, Sivonová, Monika, Dusinská, Mária, Mislanová, Csilla, Viña, José, Möller, Lennart, Hofer, Tim, Nygren, Jonas, Gremaud, Eric, Herbert, Karl, Lunec, Joseph, Collins, Andrew, Gedik, Catherine, Vaughan, Nicholas, Wood, Sharon, White, Ann, Dubois, Jacques, Rees, Jean-François, Loft, Steffen, Møller, Peter, Poulsen, Henrik, Cadet, Jean, Douki, Thierry, Ravanat, Jean-Luc, Sauvaigo, Sylvie, Faure, Henry, Morel, Isabelle, Morin, Bénédicte, Epe, Bernd, Phoa, Nicole, Hartwig, Andrea, Schwerdtle, Tanja, Dolara, Piero, Giovannelli, Lisa, Lodovici, Maura, Olinski, Ryszard, Bialkowski, Karol, Foksinski, Marek, Gackowski, Daniel, Duracková, Zdena, Hlinciková, Lucia, Korytar, Peter, Sivonová, Monika, Dusinská, Mária, Mislanová, Csilla, Viña, José, Möller, Lennart, Hofer, Tim, Nygren, Jonas, Gremaud, Eric, Herbert, Karl, and Lunec, Joseph

Abstract

The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up to resolve problems in the measurement of DNA oxidation that have resulted in varying estimates of the extent of this damage in humans. HeLa cells, sent to members for analysis, were either untreated, or treated with light in the presence of a photosensitizer to induce different amounts of 8-oxo-7,8-dihydroguanine (8-oxoGua) in DNA. Laboratories employing HPLC with electrochemical detection were able to measure the induced damage with similar efficiency; dose response gradients for seven of the eight sets of results were almost identical. GC-MS and HPLC-MS/MS, employed in three laboratories, did not convincingly detect the dose response. An alternative approach to measuring base oxidation employs the enzyme formamidopyrimidine DNA N-glycosylase (FPG) to convert 8-oxoGua to strand breaks, which are then measured by alkaline unwinding, alkaline elution, or the comet assay. Ten laboratories used this approach; five were able to detect the dose response in cells treated with photosensitizer plus light (at lower doses than for chromatographic methods, because the enzymic methods are more sensitive and less prone to spurious oxidation). Median values for 8-oxoGua (or FPG-sensitive sites) in untreated cells were 4.01 per 106 guanines for chromatographic methods, and 0.53 per 106 guanines for techniques based on FPG. © 2003 Elsevier Inc., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2003

41. Measurement of DNA oxidation in human cells by chromatographic and enzymatic methods

Author

Collins, A., Gedik, Catherine, Vaughan, Nicholas, Wood, Sharon, White, Ann, Dubois, Jacques, Rees, Jean-François, Loft, Steffen, Moller, P., Poulsen, Henrik, Cadet, Jean, Douki, Thierry, Ravanat, Jean-Luc, Sauvaigo, Sylvie, Morel, Isabelle, Morin, Bénédicte, Epe, Bernd, Phoa, Nicole, Hartwig, Andrea, Schwerdtle, Tanja, Dolara, Piero, Giovannelli, Lisa, Lodovici, Maura, Olinski, Ryszard, Bialkowski, Karol, Foksinski, Marek, Gackowski, Daniel, Duracková, Zdena, Hlinciková, Lucia, Korytar, Peter, Sivonová, Monika, Dusinská, Mária, Mislanová, Csilla, Viña, José, Möller, Lennart, Hofer, Tim, Nygren, Jonas, Gremaud, Eric, Herbert, Karl, Lunec, Joseph, Wild, Chris, Hardie, Laura, Olliver, Joanna, Smith, Elizabeth, Collins, A., Gedik, Catherine, Vaughan, Nicholas, Wood, Sharon, White, Ann, Dubois, Jacques, Rees, Jean-François, Loft, Steffen, Moller, P., Poulsen, Henrik, Cadet, Jean, Douki, Thierry, Ravanat, Jean-Luc, Sauvaigo, Sylvie, Morel, Isabelle, Morin, Bénédicte, Epe, Bernd, Phoa, Nicole, Hartwig, Andrea, Schwerdtle, Tanja, Dolara, Piero, Giovannelli, Lisa, Lodovici, Maura, Olinski, Ryszard, Bialkowski, Karol, Foksinski, Marek, Gackowski, Daniel, Duracková, Zdena, Hlinciková, Lucia, Korytar, Peter, Sivonová, Monika, Dusinská, Mária, Mislanová, Csilla, Viña, José, Möller, Lennart, Hofer, Tim, Nygren, Jonas, Gremaud, Eric, Herbert, Karl, Lunec, Joseph, Wild, Chris, Hardie, Laura, Olliver, Joanna, and Smith, Elizabeth

Abstract

ESCODD, info:eu-repo/semantics/published

Published
2003

42. Cu,Zn-superoxide dismutase deficiency in mice leads to organ-specific increase in oxidatively damaged DNA and NF-κB1 protein activity.

Author

Siomek, Agnieszka, primary, Brzoska, Kamil, primary, Sochanowicz, Barbara, primary, Gackowski, Daniel, primary, Rozalski, Rafal, primary, Foksinski, Marek, primary, Zarakowska, Ewelina, primary, Szpila, Anna, primary, Guz, Jolanta, primary, Bartlomiejczyk, Teresa, primary, Kalinowski, Bartlomiej, primary, Kruszewski, Marcin, primary, and Olinski, Ryszard, primary

Published
2010
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43. Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: An approach to consensus

Author

Collins, Andrew, Gedik, Catherine, Vaughan, Nicholas, Wood, Sharon, White, Ann, Dubois, Jacques, Duez, Pierre, Dehon, Gilles, Rees, Jean-François, Loft, Steffen, Møller, Peter, Poulsen, Henrik, Riis, Bente, Weimann, Allan, Cadet, Jean, Douki, Thierry, Ravanat, Jean-Luc, Sauvaigo, Sylvie, Faure, Henry, Morel, Isabelle, Morin, Bénédicte, Epe, Bernd, Phoa, Nicole, Hartwig, Andrea, Pelzer, Anke, Dolara, Piero, Casalini, Chiara, Giovannelli, Lisa, Lodovici, Maura, Olinski, Ryszard, Bialkowski, Karol, Foksinski, Marek, Gackowski, Daniel, Duracková, Zdena, Hlinciková, Lucia, Korytar, Peter, Sivonová, Monika, Dusinská, Mária, Mislanová, Csilla, Viña, José, Lloret, Ana, Möller, Lennart, Hofer, Tim, Nygren, Jonas, Gremaud, Eric, Herbert, Karl, Chauhan, Dina, Kelly, Frank, Dunster, Chrisi, Lunec, Joseph, Cooke, Marcus, Evans, Mark, Patel, Parul, Podmore, Ian, White, Andrea, Wild, Chris, Olliver, Joanna, Smith, Elizabeth, Collins, Andrew, Gedik, Catherine, Vaughan, Nicholas, Wood, Sharon, White, Ann, Dubois, Jacques, Duez, Pierre, Dehon, Gilles, Rees, Jean-François, Loft, Steffen, Møller, Peter, Poulsen, Henrik, Riis, Bente, Weimann, Allan, Cadet, Jean, Douki, Thierry, Ravanat, Jean-Luc, Sauvaigo, Sylvie, Faure, Henry, Morel, Isabelle, Morin, Bénédicte, Epe, Bernd, Phoa, Nicole, Hartwig, Andrea, Pelzer, Anke, Dolara, Piero, Casalini, Chiara, Giovannelli, Lisa, Lodovici, Maura, Olinski, Ryszard, Bialkowski, Karol, Foksinski, Marek, Gackowski, Daniel, Duracková, Zdena, Hlinciková, Lucia, Korytar, Peter, Sivonová, Monika, Dusinská, Mária, Mislanová, Csilla, Viña, José, Lloret, Ana, Möller, Lennart, Hofer, Tim, Nygren, Jonas, Gremaud, Eric, Herbert, Karl, Chauhan, Dina, Kelly, Frank, Dunster, Chrisi, Lunec, Joseph, Cooke, Marcus, Evans, Mark, Patel, Parul, Podmore, Ian, White, Andrea, Wild, Chris, Olliver, Joanna, and Smith, Elizabeth

Abstract

SCOPUS: re.j, info:eu-repo/semantics/published

Published
2002

44. Interlaboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine

Author

Cooke, Marcus S., primary, Barregard, Lars, additional, Mistry, Vilas, additional, Potdar, Neelam, additional, Rozalski, Rafal, additional, Gackowski, Daniel, additional, Siomek, Agnieszka, additional, Foksinski, Marek, additional, Svoboda, Peter, additional, Kasai, Hiroshi, additional, Konje, Justin C., additional, Sallsten, Gerd, additional, Evans, Mark D., additional, and Olinski, Ryszard, additional

Published
2009
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