37 results on '"Flori, Serena"'
Search Results
2. Light-independent regulation of algal photoprotection by CO2 availability
- Author
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Ruiz-Sola, M. Águila, Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz-Luque, Emanuel, Redekop, Petra, Tokutsu, Ryutaro, Küken, Anika, Tsichla, Angeliki, Kepesidis, Georgios, Allorent, Guillaume, Arend, Marius, Iacono, Fabrizio, Finazzi, Giovanni, Hippler, Michael, Nikoloski, Zoran, Minagawa, Jun, Grossman, Arthur R., and Petroutsos, Dimitris
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- 2023
- Full Text
- View/download PDF
3. Cell Biology of Organelles
- Author
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Maier, Uwe G., Moog, Daniel, Flori, Serena, Jouneau, Pierre-Henri, Falconet, Denis, Heimerl, Thomas, Kroth, Peter G., Finazzi, Giovanni, Falciatore, Angela, editor, and Mock, Thomas, editor
- Published
- 2022
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4. Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging
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Uwizeye, Clarisse, Decelle, Johan, Jouneau, Pierre-Henri, Flori, Serena, Gallet, Benoit, Keck, Jean-Baptiste, Bo, Davide Dal, Moriscot, Christine, Seydoux, Claire, Chevalier, Fabien, Schieber, Nicole L., Templin, Rachel, Allorent, Guillaume, Courtois, Florence, Curien, Gilles, Schwab, Yannick, Schoehn, Guy, Zeeman, Samuel C., Falconet, Denis, and Finazzi, Giovanni
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- 2021
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- View/download PDF
5. Light-independent regulation of algal photoprotection by CO2 availability
- Author
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Agence Nationale de la Recherche (France), European Commission, International Max Planck Research Schools, Universidad de Córdoba (España), Carnegie Institution for Science, Japan Society for the Promotion of Science, German Research Foundation, Department of Energy (US), Ruiz-Sola, M Águila [0000-0002-2281-6700], Flori, Serena [0000-0003-3407-2785], Yuan, Yizhong [0000-0003-3386-983X], Sanz-Luque, Emanuel [0000-0002-7300-9730], Redekop, Petra [0000-0002-2281-633X], Tokutsu, Ryutaro [0000-0003-2037-255X], Küken, Anika [0000-0003-1367-0719], Kepesidis, Georgios [0000-0002-1194-1987], Arend, Marius [0000-0002-9608-4960], Iacono, Fabrizio [0000-0002-3931-1231], Finazzi, Giovanni [0000-0003-0597-7075], Hippler, Michael [0000-0001-9670-6101], Minagawa, Jun [0000-0002-3028-3203], Grossman, Arthur R [0000-0002-3747-5881], Petroutsos, Dimitris [0000-0002-9656-661X], Ruiz-Sola, M Águila, Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz-Luque, Emanuel, Redekop, Petra, Tokutsu, Ryutaro, Küken, Anika, Tsichla, Angeliki, Kepesidis, Georgios, Allorent, Guillaume, Arend, Marius, Iacono, Fabrizio, Finazzi, Giovanni, Hippler, Michael, Nikoloski, Zoran, Minagawa, Jun, Grossman, Arthur R, Petroutsos, Dimitris, Agence Nationale de la Recherche (France), European Commission, International Max Planck Research Schools, Universidad de Córdoba (España), Carnegie Institution for Science, Japan Society for the Promotion of Science, German Research Foundation, Department of Energy (US), Ruiz-Sola, M Águila [0000-0002-2281-6700], Flori, Serena [0000-0003-3407-2785], Yuan, Yizhong [0000-0003-3386-983X], Sanz-Luque, Emanuel [0000-0002-7300-9730], Redekop, Petra [0000-0002-2281-633X], Tokutsu, Ryutaro [0000-0003-2037-255X], Küken, Anika [0000-0003-1367-0719], Kepesidis, Georgios [0000-0002-1194-1987], Arend, Marius [0000-0002-9608-4960], Iacono, Fabrizio [0000-0002-3931-1231], Finazzi, Giovanni [0000-0003-0597-7075], Hippler, Michael [0000-0001-9670-6101], Minagawa, Jun [0000-0002-3028-3203], Grossman, Arthur R [0000-0002-3747-5881], Petroutsos, Dimitris [0000-0002-9656-661X], Ruiz-Sola, M Águila, Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz-Luque, Emanuel, Redekop, Petra, Tokutsu, Ryutaro, Küken, Anika, Tsichla, Angeliki, Kepesidis, Georgios, Allorent, Guillaume, Arend, Marius, Iacono, Fabrizio, Finazzi, Giovanni, Hippler, Michael, Nikoloski, Zoran, Minagawa, Jun, Grossman, Arthur R, and Petroutsos, Dimitris
- Abstract
Photosynthetic algae have evolved mechanisms to cope with suboptimal light and CO2 conditions. When light energy exceeds CO2 fixation capacity, Chlamydomonas reinhardtii activates photoprotection, mediated by LHCSR1/3 and PSBS, and the CO2 Concentrating Mechanism (CCM). How light and CO2 signals converge to regulate these processes remains unclear. Here, we show that excess light activates photoprotection- and CCM-related genes by altering intracellular CO2 concentrations and that depletion of CO2 drives these responses, even in total darkness. High CO2 levels, derived from respiration or impaired photosynthetic fixation, repress LHCSR3/CCM genes while stabilizing the LHCSR1 protein. Finally, we show that the CCM regulator CIA5 also regulates photoprotection, controlling LHCSR3 and PSBS transcript accumulation while inhibiting LHCSR1 protein accumulation. This work has allowed us to dissect the effect of CO2 and light on CCM and photoprotection, demonstrating that light often indirectly affects these processes by impacting intracellular CO2 levels.
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- 2023
6. Diatom pyrenoids are encased in a protein shell that enables efficient CO2 fixation
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Shimakawa, Ginga, primary, Demulder, Manon, additional, Flori, Serena, additional, Kawamoto, Akihiro, additional, Tsuji, Yoshinori, additional, Nawaly, Hermanus, additional, Tanaka, Atsuko, additional, Tohda, Rei, additional, Ota, Tadayoshi, additional, Matsui, Hiroaki, additional, Morishima, Natsumi, additional, Okubo, Ryosuke, additional, Wietrzynski, Wojciech, additional, Lamm, Lorenz, additional, Righetto, Ricardo D, additional, Uwizeye, Clarisse, additional, Gallet, Benoit, additional, Jouneau, Pierre-Henri, additional, Gerle, Christoph, additional, Kurisu, Genji, additional, Finazzi, Giovanni, additional, Engel, Benjamin D, additional, and Matsuda, Yusuke, additional
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- 2023
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7. A systems-wide understanding of photosynthetic acclimation in algae and higher plants
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Moejes, Fiona Wanjiku, Matuszyńska, Anna, Adhikari, Kailash, Bassi, Roberto, Cariti, Federica, Cogne, Guillaume, Dikaios, Ioannis, Falciatore, Angela, Finazzi, Giovanni, Flori, Serena, Goldschmidt-Clermont, Michel, Magni, Stefano, Maguire, Julie, Le Monnier, Adeline, Müller, Kathrin, Poolman, Mark, Singh, Dipali, Spelberg, Stephanie, Stella, Giulio Rocco, Succurro, Antonella, Taddei, Lucilla, Urbain, Brieuc, Villanova, Valeria, Zabke, Claudia, and Ebenhöh, Oliver
- Published
- 2017
8. Diatoms exhibit dynamic chloroplast calcium signals in response to high light and oxidative stress
- Author
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flori, serena, primary, Dickenson, Jack, additional, Gaikwad, Trupti, additional, Cole, Isobel, additional, Smirnoff, Nicholas, additional, Helliwell, Katherine E, additional, Brownlee, Colin, additional, and Wheeler, Glen Lee, additional
- Published
- 2023
- Full Text
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9. Light-independent regulation of algal photoprotection by CO2 availability
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Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular, Human Frontiers Science Program, French National Research Agency, Prestige Marie-Curie co-financing grant, European Union (UE). H2020, Carneige Institution for Science, Marie Curie Initial Training Network Accliphot, Japan Society for the Promotion of Science, Deutsche Forschungsgemeinschaft / German Research Foundation (DFG), Ruiz Sola, M. Águila, Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz Luque, Emanuel, Redekop, Petra, Petroutsos, Dimitris, Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular, Human Frontiers Science Program, French National Research Agency, Prestige Marie-Curie co-financing grant, European Union (UE). H2020, Carneige Institution for Science, Marie Curie Initial Training Network Accliphot, Japan Society for the Promotion of Science, Deutsche Forschungsgemeinschaft / German Research Foundation (DFG), Ruiz Sola, M. Águila, Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz Luque, Emanuel, Redekop, Petra, and Petroutsos, Dimitris
- Abstract
Photosynthetic algae have evolved mechanisms to cope with suboptimal light and CO2 conditions. When light energy exceeds CO2 fixation capacity, Chlamydomonas reinhardtii activates photoprotection, mediated by LHCSR1/3 and PSBS, and the CO2 Concentrating Mechanism (CCM). How light and CO2 signals converge to regulate these processes remains unclear. Here, we show that excess light activates photoprotection- and CCM-related genes by altering intracellular CO2 concentrations and that depletion of CO2 drives these responses, even in total darkness. High CO2 levels, derived from respiration or impaired photosynthetic fixation, repress LHCSR3/CCM genes while stabilizing the LHCSR1 protein. Finally, we show that the CCM regulator CIA5 also regulates photoprotection, controlling LHCSR3 and PSBS transcript accumulation while inhibiting LHCSR1 protein accumulation. This work has allowed us to dissect the effect of CO2 and light on CCM and photoprotection, demonstrating that light often indirectly affects these processes by impacting intracellular CO2 levels.
- Published
- 2023
10. Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy
- Author
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Flori, Serena, primary, Jouneau, Pierre-Henri, additional, Gallet, Benoit, additional, Estrozi, Leandro F., additional, Moriscot, Christine, additional, Schoehn, Guy, additional, Finazzi, Giovanni, additional, and Falconet, Denis, additional
- Published
- 2018
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11. Isolation of Plastid Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum
- Author
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Schober, Alexander F., primary, Flori, Serena, additional, Finazzi, Giovanni, additional, Kroth, Peter G., additional, and Bártulos, Carolina Río, additional
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- 2018
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12. Light-independent regulation of algal photoprotection by CO2 availability
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Ruiz Sola, M. Águila, Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz Luque, Emanuel, Redekop, Petra, Petroutsos, Dimitris, Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular, Human Frontiers Science Program, French National Research Agency, Prestige Marie-Curie co-financing grant, European Union (UE). H2020, Carneige Institution for Science, Marie Curie Initial Training Network Accliphot, Japan Society for the Promotion of Science, and Deutsche Forschungsgemeinschaft / German Research Foundation (DFG)
- Abstract
Photosynthetic algae have evolved mechanisms to cope with suboptimal light and CO2 conditions. When light energy exceeds CO2 fixation capacity, Chlamydomonas reinhardtii activates photoprotection, mediated by LHCSR1/3 and PSBS, and the CO2 Concentrating Mechanism (CCM). How light and CO2 signals converge to regulate these processes remains unclear. Here, we show that excess light activates photoprotection- and CCM-related genes by altering intracellular CO2 concentrations and that depletion of CO2 drives these responses, even in total darkness. High CO2 levels, derived from respiration or impaired photosynthetic fixation, repress LHCSR3/CCM genes while stabilizing the LHCSR1 protein. Finally, we show that the CCM regulator CIA5 also regulates photoprotection, controlling LHCSR3 and PSBS transcript accumulation while inhibiting LHCSR1 protein accumulation. This work has allowed us to dissect the effect of CO2 and light on CCM and photoprotection, demonstrating that light often indirectly affects these processes by impacting intracellular CO2 levels. Human Frontiers Science Program RGP0046/2018 French National Research Agency ANR-18-CE20-0006, ANR-17-EURE-0003, ANR-15-IDEX-02 Prestige Marie-Curie co-financing grant PRESTIGE-2017-1-0028 European Union’s Horizon 2020 751039 Carnegie Institution for Science DE-SC0019417 Marie Curie Initial Training Network Accliphot FP7-PEPOPLE-2012-ITN, 316427 Japan Society for the Promotion of Science 21H04778, 21H05040 German Research Foundation HI 739/9.2
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- 2023
13. A blue-light photoreceptor mediates the feedback regulation of photosynthesis
- Author
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Petroutsos, Dimitris, Tokutsu, Ryutaro, Maruyama, Shinichiro, Flori, Serena, Greiner, Andre, Magneschi, Leonardo, Cusant, Loic, Kottke, Tilman, Mittag, Maria, Hegemann, Peter, Finazzi, Giovanni, and Minagawa, Jun
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- 2016
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14. Light-independent regulation of algal photoprotection by CO2 availability
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Ruiz-Sola, M. Águila, primary, Flori, Serena, additional, Yuan, Yizhong, additional, Villain, Gaelle, additional, Sanz-Luque, Emanuel, additional, Redekop, Petra, additional, Tokutsu, Ryutaro, additional, Kueken, Anika, additional, Tsichla, Angeliki, additional, Kepesidis, Georgios, additional, Allorent, Guillaume, additional, Arend, Marius, additional, Iacono, Fabrizio, additional, Finazzi, Giovanni, additional, Hippler, Michael, additional, Nikoloski, Zoran, additional, Minagawa, Jun, additional, Grossman, Arthur, additional, and Petroutsos, Dimitris, additional
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- 2022
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15. Energetic coupling between plastids and mitochondria drives C[O.sub.2] assimilation in diatoms
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Bailleul, Benjamin, Berne, Nicolas, Murik, Omer, Petroutsos, Dimitris, Prihoda, Judit, Tanaka, Atsuko, Villanova, Valeria, Bligny, Richard, Flori, Serena, Falconet, Denis, Krieger-Liszkay, Anja, Santabarbara, Stefano, Rappaport, Fabrice, Joliot, Pierre, Tirichine, Leila, Falkowski, Paul G., Cardol, Pierre, Bowler, Chris, and Finazzi, Giovanni
- Subjects
Diatoms -- Research ,Mitochondria -- Physiological aspects ,Photosynthesis -- Research ,Phytochemistry -- Research ,Botanical research ,Plastids -- Physiological aspects ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Diatoms are one of the most ecologically successful classes of photosynthetic marine eukaryotes in the contemporary oceans. Over the past 30 million years, they have helped to moderate Earth's climate [...]
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- 2015
16. Light-independent regulation of algal photoprotection by CO2 availability.
- Author
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Águila Ruiz-Sola, M., Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz-Luque, Emanuel, Redekop, Petra, Tokutsu, Ryutaro, Küken, Anika, Tsichla, Angeliki, Kepesidis, Georgios, Allorent, Guillaume, Arend, Marius, Iacono, Fabrizio, Finazzi, Giovanni, Hippler, Michael, Nikoloski, Zoran, Minagawa, Jun, Grossman, Arthur R., and Petroutsos, Dimitris
- Subjects
CHLAMYDOMONAS reinhardtii ,CHLAMYDOMONAS ,ALGAE ,PHOTOBIOLOGY - Abstract
Photosynthetic algae have evolved mechanisms to cope with suboptimal light and CO
2 conditions. When light energy exceeds CO2 fixation capacity, Chlamydomonas reinhardtii activates photoprotection, mediated by LHCSR1/3 and PSBS, and the CO2 Concentrating Mechanism (CCM). How light and CO2 signals converge to regulate these processes remains unclear. Here, we show that excess light activates photoprotection- and CCM-related genes by altering intracellular CO2 concentrations and that depletion of CO2 drives these responses, even in total darkness. High CO2 levels, derived from respiration or impaired photosynthetic fixation, repress LHCSR3/CCM genes while stabilizing the LHCSR1 protein. Finally, we show that the CCM regulator CIA5 also regulates photoprotection, controlling LHCSR3 and PSBS transcript accumulation while inhibiting LHCSR1 protein accumulation. This work has allowed us to dissect the effect of CO2 and light on CCM and photoprotection, demonstrating that light often indirectly affects these processes by impacting intracellular CO2 levels. Photosynthetic algae have evolved to survive in suboptimal light and CO2 conditions. Here, the authors show that depletion of CO2 can drive photoprotection and carbon acquisition even in the absence of light, that was previously believed to be indispensable for the activation of these processes. [ABSTRACT FROM AUTHOR]- Published
- 2023
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17. Distinct physiological responses of Coccolithus braarudii life cycle phases to light intensity and nutrient availability
- Author
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Langer, Gerald, primary, Jie, Vun Wen, additional, Kottmeier, Dorothee, additional, Flori, Serena, additional, Sturm, Daniela, additional, de Vries, Joost, additional, Harper, Glenn M., additional, Brownlee, Colin, additional, and Wheeler, Glen, additional
- Published
- 2022
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18. Photoprotection is regulated by light-independent CO 2 availability
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Petroutsos, Dimitris, Ruiz-Sola, M. Águila, Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz-Luque, Emanuel, Redekop, Petra, Tokutsu, Ryutaro, Kueken, Anika, Tsichla, Angeliki, Kepesidis, Georgios, Allorent, Guillaume, Arend, Marius, Iacono, Fabrizio, Finazzi, Giovanni, Hippler, Michael, Nikoloski, Zoran, Minagawa, Jun, Grossman, Arthur, Physiologie cellulaire et végétale (LPCV), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
- Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology - Abstract
Photosynthetic algae cope with suboptimal levels of light and CO 2 . In low CO 2 and excess light, the green alga Chlamydomonas reinhardtii activates a CO 2 Concentrating Mechanism (CCM) and photoprotection; the latter is mediated by LHCSR1/3 and PSBS. How light and CO 2 signals converge to regulate photoprotective responses remains unclear. Here we show that excess light activates expression of photoprotection- and CCM-related genes and that depletion of CO 2 drives these responses, even in total darkness. High CO 2 levels, derived from respiration or impaired photosynthetic fixation, repress LHCSR3 and CCM genes while stabilizing the LHCSR1 protein. We also show that CIA5, which controls CCM genes, is a major regulator of photoprotection, elevating LHCSR3 and PSBS transcript accumulation while inhibiting LHCSR1 accumulation. Our work emphasizes the importance of CO 2 in regulating photoprotection and the CCM, demonstrating that the impact of light on photoprotection is often indirect and reflects intracellular CO 2 levels.
- Published
- 2021
19. Distinct physiological responses of Coccolithus braarudii life cycle phases to light intensity and nutrient availability.
- Author
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Langer, Gerald, Jie, Vun Wen, Kottmeier, Dorothee, Flori, Serena, Sturm, Daniela, de Vries, Joost, Harper, Glenn M., Brownlee, Colin, and Wheeler, Glen
- Subjects
LIFE cycles (Biology) ,LIGHT intensity ,HAPLOIDY ,ECOLOGICAL niche ,WATER depth ,COCCOLITHOPHORES ,WINTER - Abstract
Coccolithophores feature a haplo-diplontic life cycle comprised of diploid cells producing heterococcoliths and haploid cells producing morphologically different holococcoliths. These life cycle phases of each species appear to have distinct spatial and temporal distributions in the oceans, with the heavily calcified heterococcolithophores (HET) often more prevalent in winter and at greater depths, whilst the lightly calcified holococcolithophores (HOL) are more abundant in summer and in shallower waters. The haplo-diplontic life cycle may therefore allow coccolithophores to expand their ecological niche, switching between life cycle phases to exploit conditions that are more favourable. However, coccolithophore life cycles remain poorly understood and fundamental information on the physiological differences between life cycle phases is required if we are to better understand the ecophysiology of coccolithophores. In this study, we have examined the physiology of HET and HOL phases of the coccolithophore Coccolithus braarudii in response to changes in light and nutrient availability. We found that the HOL phase was more tolerant to high light than the HET phase, which exhibited defects in calcification at high irradiances. The HET phase exhibited defects in coccolith formation under both nitrate (N) and phosphate (P) limitation, whilst no defects in calcification were detected in the HOL phase. The HOL phase grew to a higher cell density under P-limitation than N-limitation, whereas no difference was observed in the maximum cell density reached by the HET phase at these nutrient concentrations. HET cells grown under a light:dark cycle divided primarily in the dark and early part of the light phase, whereas HOL cells continued to divide throughout the 24 h period. The physiological differences may contribute to the distinct biogeographic distributions observed between life cycle phases, with the HOL phase potentially better adapted to high light, low nutrient regimes, such as those found in seasonally stratified surface waters. Coccolithus braarudii life cycle phases exhibit different physiological responses. The heavily calcified heterococcolithophores (HET) life cycle phase is more sensitive to high light. The lightly calcified holococcolithophores (HOL) life cycle phase may be better suited to growth under low phosphate availability. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
20. Light-independent regulation of algal photoprotection by CO2availability
- Author
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Ruiz-Sola, M. Águila, primary, Flori, Serena, additional, Yuan, Yizhong, additional, Villain, Gaelle, additional, Sanz-Luque, Emanuel, additional, Redekop, Petra, additional, Tokutsu, Ryutaro, additional, Kueken, Anika, additional, Tsichla, Angeliki, additional, Kepesidis, Georgios, additional, Allorent, Guillaume, additional, Arend, Marius, additional, Iacono, Fabrizio, additional, Finazzi, Giovanni, additional, Hippler, Michael, additional, Nikoloski, Zoran, additional, Minagawa, Jun, additional, Grossman, Arthur R., additional, and Petroutsos, Dimitris, additional
- Published
- 2021
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21. Plastid thylakoid architecture optimizes photosynthesis in diatoms
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Flori, Serena, Jouneau, Pierre-Henri, Bailleul, Benjamin, Gallet, Benoit, Estrozi, Leandro F, Moriscot, Christine, Bastien, Olivier, Eicke, Simona, Schober, Alexander, Bártulos, Carolina Río, Maréchal, Eric, Kroth, Peter G, Petroutsos, Dimitris, Zeeman, Samuel, Breyton, Cécile, Schoehn, Guy, Falconet, Denis, Finazzi, Giovanni, Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire d'Etude des Matériaux par Microscopie Avancée (LEMMA), Modélisation et Exploration des Matériaux (MEM), Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Physiologie membranaire et moléculaire du chloroplaste (PMMC), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC), Institut de biologie structurale (IBS - UMR 5075), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS), Plant Biochemistry, Department of Biology, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology in Zürich [Zürich] (ETH Zürich), Department of Biology, University of Konstanz, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Laboratoire d'Etude des Matériaux par Microscopie Avancée (LEMMA ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Graduate School of Chemical Biology (KoRS-CB), FRISBI within the Grenoble Partnership for Structural Biology (PSB) [ANR-10-INSB-05-02], Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Diatoms ,Chloroplasts ,Photosystem I Protein Complex ,[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,Science ,Photosystem II Protein Complex ,Thylakoids ,Article ,[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM] ,ddc:570 ,Plastids ,Photosynthesis ,Electron microscopy - Abstract
Photosynthesis is a unique process that allows independent colonization of the land by plants and of the oceans by phytoplankton. Although the photosynthesis process is well understood in plants, we are still unlocking the mechanisms evolved by phytoplankton to achieve extremely efficient photosynthesis. Here, we combine biochemical, structural and in vivo physiological studies to unravel the structure of the plastid in diatoms, prominent marine eukaryotes. Biochemical and immunolocalization analyses reveal segregation of photosynthetic complexes in the loosely stacked thylakoid membranes typical of diatoms. Separation of photosystems within subdomains minimizes their physical contacts, as required for improved light utilization. Chloroplast 3D reconstruction and in vivo spectroscopy show that these subdomains are interconnected, ensuring fast equilibration of electron carriers for efficient optimum photosynthesis. Thus, diatoms and plants have converged towards a similar functional distribution of the photosystems although via different thylakoid architectures, which likely evolved independently in the land and the ocean., Phytoplankton and plant plastids have distinct evolutionary origins and membrane organization. Here Flori et al. show that diatom photosynthetic complexes spatially segregate into interconnected subdomains within loose thylakoid stacks enabling fast diffusion of electron carriers and efficient photosynthesis
- Published
- 2017
- Full Text
- View/download PDF
22. A systems-wide understanding of photosynthetic acclimation in algae and higher plants
- Author
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Wanjiku Moejes, Fiona, Matuszyńska, Anna, Adhikari, Kailash, Bassi, Roberto, Cariti, Federica, Cogne, Guillaume, Dikaios, Joannis, Falciatore, Angela, Finazzi, Giovanni, Flori, Serena, Goldschmidt-Clermont, Michel, Magni, Stefano, Maguire, Julie, Le Monnier, Adeline, Müller, Kathrin, Poolman, Mark, Singh, Dipali, Spelberg, Stephanie, Stella, Giulio Rocco, Succurro, Antonella, Taddei, Lucilla, Urbain, Brieuc, Villanova, Valeria, Zabke, Claudia, Ebenhöh, Oliver, Wanjiku Moejes, Fiona, Matuszyńska, Anna, Adhikari, Kailash, Bassi, Roberto, Cariti, Federica, Cogne, Guillaume, Dikaios, Joannis, Falciatore, Angela, Finazzi, Giovanni, Flori, Serena, Goldschmidt-Clermont, Michel, Magni, Stefano, Maguire, Julie, Le Monnier, Adeline, Müller, Kathrin, Poolman, Mark, Singh, Dipali, Spelberg, Stephanie, Stella, Giulio Rocco, Succurro, Antonella, Taddei, Lucilla, Urbain, Brieuc, Villanova, Valeria, Zabke, Claudia, and Ebenhöh, Oliver
- Abstract
The ability of phototrophs to colonise different environments relies on robust protection against oxidative stress, a critical requirement for the successful evolutionary transition from water to land. Photosynthetic organisms have developed numerous strategies to adapt their photosynthetic apparatus to changing light conditions in order to optimise their photosynthetic yield, which is crucial for life on Earth to exist. Photosynthetic acclimation is an excellent example of the complexity of biological systems, where highly diverse processes, ranging from electron excitation over protein protonation to enzymatic processes coupling ion gradients with biosynthetic activity, interact on drastically different timescales from picoseconds to hours. Efficient functioning of the photosynthetic apparatus and its protection is paramount for efficient downstream processes, including metabolism and growth. Modern experimental techniques can be successfully integrated with theoretical and mathematical models to promote our understanding of underlying mechanisms and principles. This review aims to provide a retrospective analysis of multidisciplinary photosynthetic acclimation research carried out by members of the Marie Curie Initial Training Project, AccliPhot, placing the results in a wider context. The review also highlights the applicability of photosynthetic organisms for industry, particularly with regards to the cultivation of microalgae. It intends to demonstrate how theoretical concepts can successfully complement experimental studies broadening our knowledge of common principles in acclimation processes in photosynthetic organisms, as well as in the field of applied microalgal biotechnology.
- Published
- 2017
- Full Text
- View/download PDF
23. A systems-wide understanding of photosynthetic acclimation in algae and higher plants
- Author
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Matuszy?ska, Anna, Moejes, Fiona Wanjiku, Adhikari, Kailash, Bassi, Roberto, Cariti, Federica, Cogne, Guillaume, Dikaios, Ioannis, Falciatore, Angela, Finazzi, Giovanni, Flori, Serena, Goldschmidt-Clermont, Michel, Magni, Stefano, Maguire, Julie, Le Monnier, Adeline, Müller, Kathrin, Poolman, Mark, Singh, Dipali, Spelberg, Stephanie, Stella, Giulio Rocco, Succurro, Antonello, Taddei, Lucilla, Urbain, Brieuc, Villanova, Valeria, Zabke, Claudia, Ebenhöh, Oliver, Matuszy?ska, Anna, Moejes, Fiona Wanjiku, Adhikari, Kailash, Bassi, Roberto, Cariti, Federica, Cogne, Guillaume, Dikaios, Ioannis, Falciatore, Angela, Finazzi, Giovanni, Flori, Serena, Goldschmidt-Clermont, Michel, Magni, Stefano, Maguire, Julie, Le Monnier, Adeline, Müller, Kathrin, Poolman, Mark, Singh, Dipali, Spelberg, Stephanie, Stella, Giulio Rocco, Succurro, Antonello, Taddei, Lucilla, Urbain, Brieuc, Villanova, Valeria, Zabke, Claudia, and Ebenhöh, Oliver
- Abstract
The ability of phototrophs to colonise different environments relied on the robust protection against oxidative stress in phototrophs, a critical requirement for the successful evolutionary transition from water to land. Photosynthetic organisms have developed numerous strategies to adapt their photosynthetic apparatus to changing light conditions in order to optimise their photosynthetic yield, crucial for life to exist on Earth. Photosynthetic acclimation is an excellent example of the complexity of biological systems, in which highly diverse processes, ranging from electron excitation over protein protonation to enzymatic processes coupling ion gradients with biosynthetic activity interact on drastically different timescales, ranging from picoseconds to hours. An efficient functioning of the photosynthetic apparatus and its protection is paramount for efficient downstream processes including metabolism and growth. Modern experimental techniques can be successfully integrated with theoretical and mathematical models to promote our understanding of underlying mechanisms and principles. This Review aims to provide a retrospective analysis of multidisciplinary photosynthetic acclimation research carried out by members of the Marie Curie Initial Training Project “AccliPhot”, placing the results in a wider context. The Review also highlights the applicability of photosynthetic organisms for industry, particularly with regards to the cultivation of microalgae. It aims to demonstrate how theoretical concepts can successfully complement experimental studies broadening our knowledge of common principles in acclimation processes in photosynthetic organisms, as well as in the field of applied microalgal biotechnology.
- Published
- 2017
24. Use of fib‐sem to address the ultrastructure of the periplastidial and internal chloroplast compartments of the diatom Phaeodactylum tricornutum
- Author
-
Flori, Serena, primary, Jouneau, Pierre‐Henri, additional, Gallet, Benoit, additional, Moriscot, Christine, additional, Estrozi, Leandro, additional, Petroutsos, Dimitris, additional, Breyton, Cécile, additional, Schoehn, Guy, additional, Maréchal, Eric, additional, Finazzi, Giovanni, additional, and Falconet, Denis, additional
- Published
- 2016
- Full Text
- View/download PDF
25. The Water to Water Cycles in Microalgae.
- Author
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Curien, Gilles, Flori, Serena, Villanova, Valeria, Magneschi, Leonardo, Giustini, Cécile, Forti, Giorgio, Matringe, Michel, Petroutsos, Dimitris, Kuntz, Marcel, Finazzi, Giovanni, Curien, Gilles, Flori, Serena, Villanova, Valeria, Magneschi, Leonardo, Giustini, Cécile, Forti, Giorgio, Matringe, Michel, Petroutsos, Dimitris, Kuntz, Marcel, and Finazzi, Giovanni
- Abstract
In oxygenic photosynthesis, light produces ATP plus NADPH via linear electron transfer, i.e. the in-series activity of the two photosystems: PSI and PSII. This process, however, is thought not to be sufficient to provide enough ATP per NADPH for carbon assimilation in the Calvin-Benson-Bassham cycle. Thus, it is assumed that additional ATP can be generated by alternative electron pathways. These circuits produce an electrochemical proton gradient without NADPH synthesis, and, although they often represent a small proportion of the linear electron flow, they could have a huge importance in optimizing CO2 assimilation. In Viridiplantae, there is a consensus that alternative electron flow comprises cyclic electron flow around PSI and the water to water cycles. The latter processes include photosynthetic O2 reduction via the Mehler reaction at PSI, the plastoquinone terminal oxidase downstream of PSII, photorespiration (the oxygenase activity of Rubisco) and the export of reducing equivalents towards the mitochondrial oxidases, through the malate shuttle. In this review, we summarize current knowledge about the role of the water to water cycles in photosynthesis, with a special focus on their occurrence and physiological roles in microalgae.
- Published
- 2016
- Full Text
- View/download PDF
26. Chloroplast tomography allows revisiting diatoms photosynthesis
- Author
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Flori, Serena, primary, Petroutsos, Dimitris, additional, Falconet, Denis, additional, and Finazzi, Giovanni, additional
- Published
- 2016
- Full Text
- View/download PDF
27. Ultrastructure of the Periplastidial Compartment of the Diatom Phaeodactylum tricornutum
- Author
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Flori, Serena, primary, Jouneau, Pierre-Henri, additional, Finazzi, Giovanni, additional, Maréchal, Eric, additional, and Falconet, Denis, additional
- Published
- 2016
- Full Text
- View/download PDF
28. The Water to Water Cycles in Microalgae
- Author
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Curien, Gilles, primary, Flori, Serena, additional, Villanova, Valeria, additional, Magneschi, Leonardo, additional, Giustini, Cécile, additional, Forti, Giorgio, additional, Matringe, Michel, additional, Petroutsos, Dimitris, additional, Kuntz, Marcel, additional, and Finazzi, Giovanni, additional
- Published
- 2016
- Full Text
- View/download PDF
29. Ions channels/transporters and chloroplast regulation.
- Author
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Finazzi, Giovanni, Petroutsos, Dimitris, Tomizioli, Martino, Flori, Serena, Sautron, Emeline, Villanova, Valeria, Rolland, Norbert, Seigneurin-Berny, Daphné, Finazzi, Giovanni, Petroutsos, Dimitris, Tomizioli, Martino, Flori, Serena, Sautron, Emeline, Villanova, Valeria, Rolland, Norbert, and Seigneurin-Berny, Daphné
- Abstract
Ions play fundamental roles in all living cells and their gradients are often essential to fuel transports, to regulate enzyme activities and to transduce energy within and between cells. Their homeostasis is therefore an essential component of the cell metabolism. Ions must be imported from the extracellular matrix to their final subcellular compartments. Among them, the chloroplast is a particularly interesting example because there, ions not only modulate enzyme activities, but also mediate ATP synthesis and actively participate in the building of the photosynthetic structures by promoting membrane-membrane interaction. In this review, we first provide a comprehensive view of the different machineries involved in ion trafficking and homeostasis in the chloroplast, and then discuss peculiar functions exerted by ions in the frame of photochemical conversion of absorbed light energy.
- Published
- 2015
- Full Text
- View/download PDF
30. Ions channels/transporters and chloroplast regulation
- Author
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Finazzi, Giovanni, primary, Petroutsos, Dimitris, additional, Tomizioli, Martino, additional, Flori, Serena, additional, Sautron, Emeline, additional, Villanova, Valeria, additional, Rolland, Norbert, additional, and Seigneurin-Berny, Daphné, additional
- Published
- 2015
- Full Text
- View/download PDF
31. The Velocity of Light Intensity Increase Modulates the Photoprotective Response in Coastal Diatoms
- Author
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Giovagnetti, Vasco, primary, Flori, Serena, additional, Tramontano, Ferdinando, additional, Lavaud, Johann, additional, and Brunet, Christophe, additional
- Published
- 2014
- Full Text
- View/download PDF
32. Energetic coupling between plastids and mitochondria drives CO2 assimilation in diatoms.
- Author
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Bailleul, Benjamin, Berne, Nicolas, Murik, Omer, Petroutsos, Dimitris, Prihoda, Judit, Tanaka, Atsuko, Villanova, Valeria, Bligny, Richard, Flori, Serena, Falconet, Denis, Krieger-Liszkay, Anja, Santabarbara, Stefano, Rappaport, Fabrice, Joliot, Pierre, Tirichine, Leila, Falkowski, Paul G., Cardol, Pierre, Bowler, Chris, and Finazzi, Giovanni
- Subjects
PLASTIDS ,MITOCHONDRIA ,DIATOMS ,CARBON dioxide ,ABSORPTION ,LITHOSPHERE ,CARBON fixation ,ADENOSINE triphosphatase - Abstract
Diatoms are one of the most ecologically successful classes of photosynthetic marine eukaryotes in the contemporary oceans. Over the past 30 million years, they have helped to moderate Earth's climate by absorbing carbon dioxide from the atmosphere, sequestering it via the biological carbon pump and ultimately burying organic carbon in the lithosphere. The proportion of planetary primary production by diatoms in the modern oceans is roughly equivalent to that of terrestrial rainforests. In photosynthesis, the efficient conversion of carbon dioxide into organic matter requires a tight control of the ATP/NADPH ratio which, in other photosynthetic organisms, relies principally on a range of plastid-localized ATP generating processes. Here we show that diatoms regulate ATP/NADPH through extensive energetic exchanges between plastids and mitochondria. This interaction comprises the re-routing of reducing power generated in the plastid towards mitochondria and the import of mitochondrial ATP into the plastid, and is mandatory for optimized carbon fixation and growth. We propose that the process may have contributed to the ecological success of diatoms in the ocean. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
33. Plastid thylakoid architecture optimizes photosynthesis in diatoms
- Author
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Flori, Serena, Jouneau, Pierre-Henri, Bailleul, Benjamin, Gallet, Benoit, Estrozi, Leandro F., Moriscot, Christine, Bastien, Olivier, Eicke, Simona, Schober, Alexander, Bártulos, Carolina Río, Maréchal, Eric, Kroth, Peter G., Petroutsos, Dimitris, Zeeman, Samuel C., Breyton, Cécile, Schoehn, Guy, Falconet, Denis, and Finazzi, Giovanni
- Subjects
Electron microscopy ,14. Life underwater ,Photosynthesis - Abstract
Photosynthesis is a unique process that allows independent colonization of the land by plants and of the oceans by phytoplankton. Although the photosynthesis process is well understood in plants, we are still unlocking the mechanisms evolved by phytoplankton to achieve extremely efficient photosynthesis. Here, we combine biochemical, structural and in vivo physiological studies to unravel the structure of the plastid in diatoms, prominent marine eukaryotes. Biochemical and immunolocalization analyses reveal segregation of photosynthetic complexes in the loosely stacked thylakoid membranes typical of diatoms. Separation of photosystems within subdomains minimizes their physical contacts, as required for improved light utilization. Chloroplast 3D reconstruction and in vivo spectroscopy show that these subdomains are interconnected, ensuring fast equilibration of electron carriers for efficient optimum photosynthesis. Thus, diatoms and plants have converged towards a similar functional distribution of the photosystems although via different thylakoid architectures, which likely evolved independently in the land and the ocean., Nature Communications, 8, ISSN:2041-1723
34. Diatoms exhibit dynamic chloroplast calcium signals in response to high light and oxidative stress.
- Author
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Flori S, Dickenson J, Gaikwad T, Cole I, Smirnoff N, Helliwell K, Brownlee C, and Wheeler G
- Abstract
Diatoms are a group of silicified algae that play a major role in marine and freshwater ecosystems. Diatom chloroplasts were acquired by secondary endosymbiosis and exhibit important structural and functional differences from the primary plastids of land plants and green algae. Many functions of primary plastids, including photoacclimation and inorganic carbon acquisition, are regulated by calcium-dependent signalling processes. Calcium signalling has also been implicated in the photoprotective responses of diatoms; however, the nature of calcium elevations in diatom chloroplasts and their wider role in cell signalling remains unknown. Using genetically encoded calcium indicators, we find that the diatom Phaeodactylum tricornutum exhibits dynamic calcium elevations within the chloroplast stroma. Stromal calcium ([Ca2+]str) acts independently from the cytosol and is not elevated by stimuli that induce large cytosolic calcium ([Ca2+]cyt) elevations. In contrast, high light and exogenous hydrogen peroxide (H2O2) induce large, sustained [Ca2+]str elevations that are not replicated in the cytosol. Measurements using the fluorescent H2O2 sensor roGFP2-Oxidant Receptor Peroxidase 1 (Orp1) indicate that [Ca2+]str elevations induced by these stimuli correspond to the accumulation of H2O2 in the chloroplast. [Ca2+]str elevations were also induced by adding methyl viologen, which generates superoxide within the chloroplast, and by treatments that disrupt non-photochemical quenching (NPQ). The findings indicate that diatoms generate specific [Ca2+]str elevations in response to high light and oxidative stress that likely modulate the activity of calcium-sensitive components in photoprotection and other regulatory pathways., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
- Published
- 2024
- Full Text
- View/download PDF
35. Isolation of Plastid Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum.
- Author
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Schober AF, Flori S, Finazzi G, Kroth PG, and Bártulos CR
- Subjects
- Cells, Cultured, Centrifugation, Density Gradient, Microscopy, Fluorescence, Workflow, Cell Fractionation methods, Diatoms metabolism, Plastids metabolism
- Abstract
The so-called "complex" plastids from diatoms possessing four envelope membranes are a typical feature of algae that arose from secondary endosymbiosis. Studying isolated plastids from these algae may allow answering a number of fundamental questions regarding diatom photosynthesis and plastid functionality. Due to their complex architecture and their integration into the cellular endoplasmic reticulum (ER) system, their isolation though is still challenging. In this work, we report a reliable isolation technique that is applicable for the two model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum. The resulting plastid-enriched fractions are of homogenous quality, almost free from cellular contaminants, and feature structurally intact thylakoids that are capable to perform oxygenic photosynthesis, though in most cases they seem to lack most of the stromal components as well as plastid envelopes.
- Published
- 2018
- Full Text
- View/download PDF
36. Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy.
- Author
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Flori S, Jouneau PH, Gallet B, Estrozi LF, Moriscot C, Schoehn G, Finazzi G, and Falconet D
- Subjects
- Arabidopsis metabolism, Arabidopsis ultrastructure, Imaging, Three-Dimensional, Microscopy, Confocal, Microscopy, Electron, Molecular Imaging, Plastids metabolism, Plastids ultrastructure
- Abstract
Internal chloroplast structures present complex and various characteristics, which are still largely undetermined due to insufficient imaging investigation. Information on chloroplast morphology has traditionally been collected using light microscopy (LM), confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) techniques. However, recent technological progresses in the field of microscopy have made it possible to visualize the internal structure of chloroplast in far greater detail and in 3D. Here we recapitulate protocols to visualize chloroplasts from Arabidopsis leaves and Phaeodactylum tricornutum cells with confocal and transmission electron microscopy together with a new technique using a focused ion beam-scanning electron microscope (FIB-SEM) allowing for 3D imaging.
- Published
- 2018
- Full Text
- View/download PDF
37. Plastid thylakoid architecture optimizes photosynthesis in diatoms.
- Author
-
Flori S, Jouneau PH, Bailleul B, Gallet B, Estrozi LF, Moriscot C, Bastien O, Eicke S, Schober A, Bártulos CR, Maréchal E, Kroth PG, Petroutsos D, Zeeman S, Breyton C, Schoehn G, Falconet D, and Finazzi G
- Subjects
- Chloroplasts metabolism, Diatoms metabolism, Photosystem I Protein Complex metabolism, Photosystem II Protein Complex metabolism, Diatoms physiology, Photosynthesis physiology, Plastids metabolism, Thylakoids metabolism
- Abstract
Photosynthesis is a unique process that allows independent colonization of the land by plants and of the oceans by phytoplankton. Although the photosynthesis process is well understood in plants, we are still unlocking the mechanisms evolved by phytoplankton to achieve extremely efficient photosynthesis. Here, we combine biochemical, structural and in vivo physiological studies to unravel the structure of the plastid in diatoms, prominent marine eukaryotes. Biochemical and immunolocalization analyses reveal segregation of photosynthetic complexes in the loosely stacked thylakoid membranes typical of diatoms. Separation of photosystems within subdomains minimizes their physical contacts, as required for improved light utilization. Chloroplast 3D reconstruction and in vivo spectroscopy show that these subdomains are interconnected, ensuring fast equilibration of electron carriers for efficient optimum photosynthesis. Thus, diatoms and plants have converged towards a similar functional distribution of the photosystems although via different thylakoid architectures, which likely evolved independently in the land and the ocean.
- Published
- 2017
- Full Text
- View/download PDF
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