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ETHNOPHARMACOLOGICAL RELEVANCE: Preparation from leaves of Cordia americana have been widely used in traditional medicine in South Brazil to treat wounds and various inflammations. AIM OF THE STUDY: The objective of this work was to identify the effective compounds in the ethanolic extract prepared from the leaves of Cordia americana, which is used in traditional South Brazilian medicine as anti-inflammatory and wound healing remedy. MATERIALS AND METHODS: Isolation and structure elucidation techniques were performed in order to identify the compounds of Cordia americana and HPLC analysis was used for the quantification. The major constituent and the ethanolic extract were investigated for inhibition of 5-lipoxygenase, p38[alpha] MAPK, TNF[alpha] release and NF-[kappa]B as well as in the fibroblast scratch assay. RESULTS: Rosmarinic acid (1) was identified as the major compound with an amount of 8.44% in the ethanolic extract of the leaves of Cordia americana. The ethanolic extract as well as (1) exhibited the highest inhibitory effects on 5-lipoxygenase (IC(50)=0.69 and 0.97 µg/mL, resp., IC(50) of BWA4C as reference: 0.3 µM) and p38[alpha] (IC(50)=3.25 and 1.16 µg/mL, resp., IC(50) of SB203580 as reference: 0.046 µM) and moderate inhibitory effects on TNF[alpha] release. Slight effects were observed in the fibroblast scratch assay. CONCLUSIONS: This study increases our knowledge on the effective compound in Cordia americana and supports its use in traditional medicine. We demonstrated for the first time pharmacological effects of Cordia americana and we provide evidences for a crucial role of rosmarinic acid as the major key player. [ABSTRACT FROM AUTHOR]

Herein, goji berries were pretreated with sodium carbonate (Na 2 CO 3 ) and then dried via ultrasound-assisted air drying or microwave drying. Water migration and phenolic chemistry of goji berries were studied under drying. A three-dimensional ellipsoid water transport model, accounting for porosity and temperature fluctuations, was established to explore the intricacies of the drying mechanism. Generally, microwave drying promoted interior water transport compared to ultrasound drying. Among all the drying methods, microwave drying at 240 W (MW-240 W) exhibited the highest D e (from 7.34 × 10 -9 to 9.61 × 10 -9 m 2 /s) and k c (6.78 × 10 -4 m/s) values. The goji berries received a considerably high water content gradient between its surface and center within the first 2 s of all the drying treatments. Microwave drying diminished the water content gradient earlier than air drying and ultrasound-assisted air drying treatments. Furthermore, most correlations observed among phenolics, oxidase activity, and cell wall pectin did not align with the established theories, highlighting the highly nonlinear nature of phenolic chemistry during goji berry drying. This study provides a three-dimensional model to study the mass transfer mechanism of goji berries and analyzes the evolution of polyphenols during the drying process., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Microwave-induced combustion (MIC) was proposed in this study for honey decomposition aiming for As, Cd, Hg, and Pb determination by inductively coupled plasma mass spectrometry (ICP-MS). Sample mass (up to 1.0 g), absorbing solution (0.5 to 14.4 mol L -1 HNO 3 , and H 2 O), heating program, and combustion aids were evaluated. The Eurachem guidelines were used for method validation. The proposed method enabled combustion of a high sample mass (0.8 g of honey, with 0.4 g of microcrystalline cellulose and 100 µL of 6 mol L -1 NH 4 NO 3 ) using 6 mL of an absorbing solution consisting of 1 mol L -1 HNO 3 , which resulted in low residual carbon in solution (< 25 mg L -1 ). Honey samples from different geographical origins were analyzed. Results showed no significant difference in comparison to other two microwave decomposition methods, based on microwave-assisted wet digestion with single reaction chamber (MAWD-SRC) and microwave-assisted wet digestion (MAWD). Standard addition experiments resulted in recoveries higher than 98%. The limits of detection ranged from 1.10 (As) to 4.60 ng g -1 (Pb). In addition to using only diluted reagents and resulting in digests virtually free of interferences, the proposed method was faster (< 30 min) than most of those presented in the literature., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)

In this initial study, the impact of thermosonication as an alternative to the traditional fusion in Brazilian cheese spread (Requeijão Cremoso) manufacture was investigated. The effect of ultrasound (US) power was evaluated considering various aspects such as gross composition, microstructure, texture, rheology, color, fatty acid composition, and volatile compounds. A 13 mm US probe operating at 20 kHz was used. The experiment involved different US power levels (200, 400, and 600 W) at 85 °C for 1 min, and results were compared to the conventional process in the same conditions (85 °C for 1 min, control treatment). The texture became softer as ultrasound power increased from 200 to 600 W, which was attributed to structural changes within the protein and lipid matrix. The color of the cheese spread also underwent noticeable changes for all US treatments, and treatment at 600 W resulted in increased lightness but reduced color intensity. Moreover, the fatty acid composition of the cheese spread showed variations with different US power, with samples treated at 600 W showing lower concentrations of saturated and unsaturated fatty acids, as well as lower atherogenicity and thrombogenicity indexes, indicating a potentially healthier product. Volatile compounds were also influenced by US, with less compounds being identified at higher powers, especially at 600 W. This could indicate possible degradation, which should be evaluated in further studies regarding US treatment effects on consumer perception. Hence, this initial work demonstrated that thermosonication might be interesting in the manufacture of Brazilian cheese spread, since it can be used to manipulate the texture, color and aroma of the product in order to improve its quality parameters., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

In the context of grain storage, impurities and soybeans defects in soybeans can significantly impact the equilibrium moisture content. This, cause moisture migration and heating of the stored product, leading to increased respiratory activity. Furthermore, temperature measurements within stored grain mass do not provide sufficient information for effective grain quality monitoring, primarily due to the grains excellent thermal insulating properties. To address this issue, we propose a different approach: monitoring the equilibrium moisture content and CO 2 concentration as indicators of soybean respiration within the intergranular spaces of the stored grain mass. This study propose monitoring the CO 2 concentration in the intergranular air along with environmental variables for early detection of physicochemical and morphological changes in soybeans stored in vertical silos using near infrared spectroscopy, X-ray diffraction and scanning electron microscopy. Thermogravimetry and spectrometry analyses revealed that the interrelationships among variables had a direct impact on soybean quality attributes. Specifically, the presence of soybeans with 5.2 % impurities led to an increased in respiration rates, resulting in a CO 2 concentration of up to 5000 ppm and the consumption of up to 3.6 % of dry matter. Consequently, there were changes in the percentage of ash, proteins, fibers, and oils compositions. These findings highlight the potential for indirect assessments, enabling the prediction of physicochemical quality and contamination of soybeans stored in vertical silos through continuous monitoring of CO 2 concentration and equilibrium moisture content., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The use of pyrohydrolysis as a sample preparation method for further halogen determination is extensively discussed in this review, covering studies published in the last 30 years. This method is compatible with both organic (such as cellulose, fossil fuels, carbon nanotubes, and graphite) and inorganic (such as rocks, silicates, alumina, and nuclear fuels) matrices. It has also been used for samples with different organic matter content, such as coal, mineral supplements, and soil. Sample masses vary greatly and are dependent on organic matter content in the samples, ranging from 50 mg to up to 500 mg for organic samples, and up to 4 g to inorganic samples. Different additives, such as V 2 O 5 and cellulose, or flame retardants, such as silica, could also be used to improve analyte recovery using pyrohydrolysis. Dilute alkaline solutions or even water have been used as absorbing solutions, with mainly NaOH, NH 4 OH, and mixtures of NaHCO 3 and Na 2 CO 3 being applied. Furthermore, pyrohydrolysis is compatible with detection techniques such as ion chromatography, inductively coupled plasma mass spectrometry, ion selective electrode, inductively coupled plasma optical emission spectrometry, energy-dispersive X-ray fluorescence spectrometry, spectrophotometry, and isotope ratio mass spectrometry. Other advantages usually related to this method are the low residual carbon concentration of digests and the low residue generation. A critical comparison with alkaline extraction, alkaline fusion, Schöniger oxygen flask combustion, combustion bomb and microwave-induced combustion is also provided., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

This work presents the first report of the application of microwave-induced combustion in disposable vessels (MIC-DV) as a simple and fast sample preparation step for fluoride determination by ion-selective electrode (ISE) in leguminous seeds and oilseeds samples. Several experimental conditions of MIC-DV were evaluated, such as sample mass, absorbing solution, volume of combustion igniter, purging time with oxygen, and the use of successive combustion cycles. The accuracy of MIC-DV/ISE method was evaluated by comparing the results obtained by microwave-induced combustion and determination by ion chromatography, with agreements ranged from 94 to 106%. The method allowed to achieve relatively low limits of quantification (8.3 to 16 µg g -1 ) comparing to AOAC method (40 µg g -1 ), besides the use of small amounts of reagents, less handling of digests, making the method greener and with portable features comparing to other methods., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Honey is a food product consumed all over the world. Besides its nutritional properties, honey presents antibacterial, antioxidant, and wound-healing properties. To ensure that the final product meets qualitative and microbiological standards, honey treatment is of great importance. Conventional honey treatment is based on the heating of honey samples for decrystallization and bacteria and yeast inactivation. However, conventional heating can cause negative effects on honey quality, such as the formation of toxic compounds, reduction of enzyme activity, and loss of antioxidant and antimicrobial properties. The application of ultrasonic waves has demonstrated interesting effects on honey processing. Ultrasound (US) treatment can lead to the fragmentation of glucose crystals in crystalized honey and has little effect on its properties. In addition to inactivating microorganisms, US-assisted honey processing also preserves phenolic compounds content and antimicrobial properties. However, there is still limited information about honey sonication. The aim of the present review is to comprehensively show the possibilities of US application in honey processing and its effects on honey properties., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The use of phytochemicals as natural food additives is a topic of interest for both academic and food industry communities. However, many of these substances are sensitive to environmental conditions. For this reason, encapsulation is usually performed prior to incorporation into food products. In this sense, ultrasound-assisted encapsulation is an emerging technique that has been gaining attention in this field, bringing important advantages for the production of functional food products. This review article covered applications published in the last five years (from 2019 to 2023) on the use of ultrasound to encapsulate phytochemicals for further incorporation into food. The ultrasound mechanisms for encapsulation, its parameters, such as reactor configuration, frequency, and power, and the use of ultrasound technology, along with conventional encapsulation techniques, were all discussed. Additionally, the main challenges of existing methods and future possibilities were discussed. In general, ultrasound-assisted encapsulation has been considered a great tool for the production of smaller capsules with a lower polydispersity index. Encapsulated materials also present a higher bioavailability. However, there is still room for further developments regarding process scale-up for industrial applications. Future studies should also focus on incorporating produced capsules in model food products to further assess their stability and sensory properties.

Ultrasonic waves can induce physical and chemical changes in liquid media via acoustic cavitation. Various applications have benefitted from utilizing these effects, including but not limited to the synthesis of functional materials, emulsification, cleaning, and processing. Several books and review articles in the public domain cover both fundamental and applied aspects of ultrasonics and sonochemistry. The Editors of the Ultrasonics Sonochemistry journal possess diverse expertise in this field, from theoretical and experimental aspects of acoustic cavitation to materials synthesis, environmental remediation, and sonoprocessing. This article provides Editors' perspectives on various aspects of ultrasonics and sonochemistry that may benefit students and early career researchers., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

A simple, fast and promising sample preparation method based on microwave-induced combustion in disposable vessels (MIC-DV) was developed for Cl and S determination in crude oil by inductively coupled plasma optical emission spectrometry (ICP-OES). The MIC-DV consists of a new approach of conventional microwave-induced combustion (MIC). For the combustion, crude oil was pipetted on a disk of filter paper and placed on a quartz holder, followed by the addition of igniter solution (40 μL of 10 mol L -1  NH 4 NO 3 ). The quartz holder was inserted into a commercial 50 mL disposable polypropylene vessel containing the absorbing solution, which was then inserted in an aluminium rotor. The combustion occurs under atmospheric pressure in a domestic microwave oven not compromising the operator's safety. The following parameters of combustion were evaluated: type, concentration and volume of absorbing solution, sample mass and the possibility of performing consecutive combustion cycles. Using MIC-DV, up to 10 mg of crude oil were efficiently digested, using 2.5 mL of ultrapure H 2 O as absorbing solution. Moreover, up to 5 consecutive combustion cycles were possible without analyte losses, reaching a total sample mass of 50 mg. The MIC-DV method was validated according to Eurachem Guide recommendations. Results obtained for Cl and S by MIC-DV were in agreement with those obtained using conventional MIC, as well as those obtained for S in a certified reference material of crude oil (NIST 2721). Analyte spike recovery experiments were performed and recoveries at three concentration levels ranged from 99 to 101% for Cl and from 95 to 97% for S, indicating a good accuracy. The limit of quantification achieved by ICP-OES after MIC-DV were 73 and 50 μg g -1 for Cl and S respectively, applying 5 consecutive combustion cycles., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Vassobia breviflora belongs to the Solanaceae family, possessing biological activity against tumor cells and is a promising alternative for therapy. The aim of this investigation was to determine the phytochemical properties V. breviflora using ESI-ToF-MS. The cytotoxic effects of this extract were examined in B16-F10 melanoma cells and the relationship if any to purinergic signaling was involved. The antioxidant activity of total phenols, (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) was analyzed, as well as production of reactive oxygen species (ROS) and nitric oxide (NO) was determined. Genotoxicity was assessed by DNA damage assay. Subsequently, the structural bioactive compounds were docked against purinoceptors P2X 7 and P2Y 1 receptors. The bioactive compounds found in V. breviflora were N -methyl-(2S,4 R)- trans -4-hydroxy-L-proline, calystegine B, 12-O-benzoyl- tenacigenin A and bungoside B. In vitro cytotoxicity was demonstrated at concentration ranges of 0.1-10 mg/ml, and plasmid DNA breaks only at the concentration of 10 mg/ml. V. breviflora extracts affected hydrolysis by ectoenzymes, such as ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) and ectoadenosine deaminase (E-ADA) which control levels of degradation and formation of nucleosides and nucleotides. In the presence of substrates ATP, ADP, AMP and adenosine, the activities of E-NTPDase, 5´-NT or E-ADA were significantly modulated by V. breviflora . N -methyl-(2S,4 R)- trans -4-hydroxy- L -proline presented higher binding affinity (according to receptor-ligand complex estimated binding affinity as evidenced by ∆G values) to bind to both P2X 7 and P2Y 1 purinergic receptors.Our results suggest a putative interaction of V. breviflora bioactive compounds with growth inhibitory potential in B16-F10 melanoma and suggest that may be considered as promising compounds in melanoma and cancer treatment.

Drying rice in a single layer in a silo-dryer-aerator allows uniform drying. The objective of this study was to evaluate the physical, physicochemical, and morphological quality of rice grain cultivars (IRGA 424, BRS Pampeira, and Guri INTA) in the lower (initial time) and upper (final time) layers in a silo-dryer-aerator, employing single-layer loading at low temperatures, using the methods of near-infrared spectroscopy, X-ray diffraction analysis, scanning electron microscopy, and multivariate statistical analysis. Drying rice in silo-dryer-aerator attenuated the moisture diffusivity in the grains, minimizing its effects on the physical, physicochemical, and morphological properties of the grains. However, the physicochemical constituents and morphology of starch were preserved by the low drying temperatures, mainly in the lower layers throughout the 2-month drying. The rice grains of the Guri INTA and BRS Pampeira cultivars were the most resistant to drying and showed greater uniformity on the final quality., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)

Microglial activation has been associated to the physiopathology of neurodegenerative diseases, such as schizophrenia, and can occur during inflammation and oxidative stress. Pharmacological treatment is associated with severe side effects, and studies for use of plant extracts may offer alternatives with lower toxicity. Harpagophytum procumbens (HP) is a plant known for its anti-inflammatory properties. In the present study, we characterized the ethyl acetate fraction of HP (EAF HP) by ESI-ToF-MS and investigated the effects EAF HP in a lipopolysaccharide (LPS) induced inflammation model on microglial cells (BV-2 lineage). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), DCFH-DA (2',7'-dichlorofluorescein diacetate) and cell cycle flow cytometer analysis were performed. In vivo was investigated the amphetamine-induced psychosis model through behavioral (locomotor and exploratory activities, stereotypies and working memory) and biochemical (DCFH-DA oxidation and protein thiols) parameters in cortex and striatum of mice. EAF HP reduced activation and proliferation of microglial cells in 48 h (300 µg/mL) and in 72 h after treatments (50-500 µg/mL). Reactive oxygen species levels were lower at the concentration of 100 µg/mL EAF HP. We detected a modulatory effect on the cell cycle, with reduction of cells in S and G2/M phases. In mice, the pre-treatment with EAF HP, for 7 days, protected against positive and cognitive symptoms, as well as stereotypies induced by amphetamine. No oxidative stress was observed in this amphetamine-induced model of psychosis. Such findings suggest that EAF HP can modulate the dopaminergic neurotransmission and be a promising adjuvant in the treatment of locomotor alterations, cognitive deficits, and neuropsychiatric disorders., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

In this study, a miniaturized liquid-liquid extraction (LLE) method for pre-concentration of Na, K, Ca, and Mg in crude oil was proposed. Analytes in crude oil were quantitatively extracted to the aqueous phase, followed by flame atomic absorption spectrometry (FAAS) determination. The following parameters were evaluated: type of extraction solution, sample mass, heating temperature and time, stirring time, centrifugation time, and the use of toluene and chemical demulsifier. Accuracy was evaluated by comparing the results obtained by the proposed method (LLE-FAAS) with those obtained after high-pressure microwave-assisted wet digestion and FAAS determination (reference values). No statistical difference was observed between the reference values and those using the optimized conditions for LLE-FAAS: 2.5 g of sample; 1000 μL of 2 mol L -1 HNO 3 , 50 mg L -1 of chemical demulsifier in 500 μL of toluene, 10 min of heating at 80 °C, 60 s of stirring, and 10 min of centrifugation. Relative standard deviations were lower than 6%. The limits of quantification (LOQ) were 1.2, 1.5, 5.0, and 0.50 μg g -1 for Na, K, Ca, and Mg, respectively. The proposed miniaturized LLE method presents several advantages, such as ease-of-use, high throughput (up 10 samples can be processed per 1 h), uses a high sample mass reaching low LOQs. In addition, the use of a diluted solution for extraction reduces the amount of reagents (around 40 times) and consequently laboratory residue generation, becoming an environmental friendly method. Suitable LOQs were achieved for analyte determination at low concentration even using a simple and low-cost sample preparation system (miniaturized LLE method) and a relatively low-cost determination technique (FAAS), avoiding the use of microwave ovens and more sensitivity techniques, which are required for routine analyses., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests Rochele Sogari Picoloto reports equipment, reagents or supplies was provided by Federal University of Santa Maria. Rochele Sogari Picoloto reports a relationship with Federal University of Santa Maria that includes: employment., (Copyright © 2023 Elsevier B.V. All rights reserved.)

In this study, a novel hydrodynamic cavitation unit combined with a glow plasma discharge system (HC-GPD) was proposed for the degradation of pharmaceutical compounds in drinking water. Metronidazole (MNZ), a commonly used broad-spectrum antibiotic, was selected to demonstrate the potential of the proposed system. Cavitation bubbles generated by hydrodynamic cavitation (HC) can provide a pathway for charge conduction during glow plasma discharge (GPD). The synergistic effect between HC and GPD promotes the production of hydroxyl radicals, emission of UV light, and shock waves for MNZ degradation. Sonochemical dosimetry provided information on the enhanced formation of hydroxyl radicals during glow plasma discharge compared to hydrodynamic cavitation alone. Experimental results showed a MNZ degradation of 14% in 15 min for the HC alone (solution initially containing 300 × 10 -6 mol L -1 MNZ). In experiments with the HC-GPD system, MNZ degradation of 90% in 15 min was detected. No significant differences were observed in MNZ degradation in acidic and alkaline solutions. MNZ degradation was also studied in the presence of inorganic anions. Experimental results showed that the system is suitable for the treatment of solutions with conductivity up to 1500 × 10 -6  S cm -1 . The results of sonochemical dosimetry showed the formation of oxidant species of 0.15 × 10 -3  mol H 2 O 2 L -1 in the HC system after 15 min. For the HC-GPD system, the concentration of oxidant species after 15 min reached 13 × 10 -3  molH 2 O 2 L -1 . Based on these results, the potential of combining HC and GPD systems for water treatment was demonstrated. The present work provided useful information on the synergistic effect between hydrodynamic cavitation and glow plasma discharge and their application for the degradation of antibiotics in drinking water., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

This study evaluated the combination of high-power ultrasound (HPU), micronized salt (MS), and low KCl levels as a strategy to produce reduced sodium Bologna-type sausages. Samples with 50% NaCl reduction were produced with regular salt (RS) or MS and 0.5% KCl. The sausages were sonicated for 0 or 27 min in an ultrasonic bath (25 kHz, 60% amplitude, normal mode, 20 °C) immediately after filling. The sodium reformulation strategy was effective in compensating for the defects in the emulsion stability and texture profile caused by the NaCl reduction. Besides, the combination of HPU, MS, and KCl did not cause major impacts on the evolution of pH, Eh, and TBARS values of the sausages during storage (21 days at 4 °C). The use of MS and KCl also allowed a reduction by 50% of the NaCl content (< 42% Na; Na/K ratio: 1.2 to 1.3) of the samples without affecting the salty taste, which was enhanced by the HPU treatment., Competing Interests: Declaration of Competing Interest The author have no conflicts of interest for disclose to paper entitled “High-power ultrasound, micronized salt, and low KCl level: An effective strategy to reduce the NaCl content of Bologna-type sausages by 50%”., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

In this work, a continuous flow extraction system assisted by ultrasound (US) was developed for the extraction of Cr(III) from residual tanned leather shavings. US energy was delivered into the system by a tubular applicator (clamp-on tube US applicator). The effect of the US energy was investigated at 20 kHz of frequency and electrical input power of 75, 150, 300 and 600 W. Residence time and temperature profile were also evaluated. It was observed that the internal temperature profile was affected by the presence of US and inverted in comparison with the conditions without US. In this way, the temperature profile generated by the US was reproduced by using electrical resistances in order to compare the obtained results. The US intensity was measured using a hydrophone connected to a sound pressure meter. The use of the US did not alter the dynamic behavior of the system but increased the extraction efficiency when compared to the silent condition. US power above 75 W did not lead to increased extraction efficiency, when the residence time was 30 min. However, when 60 min of residence time were employed, the optimized US power was 150 W, resulting in an extraction efficiency of 71.7 ± 0.7 %, about 28 % higher when compared to the silent condition in the same temperature and other conditions. The US energy allowed a reduction in processing time and operational temperature when compared to the silent condition with the same temperature profile. The overall energy consumption with US was similar or lower than that observed without US, showing the feasibility of the proposed extraction system., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

Background: Previous studies have suggested that guarana ( Paullinia cupana ) and açai ( Euterpe oleracea ) have antioxidant, anti-inflammatory, and proliferative properties, indicating their potential therapeutic action in wound healing. We produced a conjugated guarana-açai (GA) extract and tested its healing action on earthworms ( Eisenia fetida ) subjected to tail amputation by surgical incision., Methods: Extract from roasted guarana seeds and fresh açai seed berries was produced. The antioxidant and genoprotective capacity of GA extract was tested. The concentration with the most remarkable healing potential was used in subsequent tests. The last three posterior segments of the clitellate earthworm tail reared under standardized conditions were surgically amputated. Next, topical PBS or GA extract was applied to the surgical wound. The rate of cell migration and tissue regeneration at the local wound site was histologically evaluated after the procedure. Expression of the SOX4 gene that acts in epithelial-to-mesenchymal transition was determined by RT-qPCR., Results: Sixteen bioactive molecules, including some previously described substances, were identified. All tested concentrations exhibited antioxidant and genoprotective effects. The GA extract accelerated the healing processes as observed through macroscopic and histological analyses and increased expression of SOX4., Conclusion: The GA extract has a potential role in the healing of surgically induced wounds., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper., (Copyright © 2022 Fellipe D. Felin et al.)

The effects of thermosonication processing (TS, 90 °C, ultrasound powers of 200, 400, and 600 W) on the quality parameters of Jamun fruit dairy dessert compared to conventional heating processing (high-temperature short time, (HTST), 90 °C/20 s) were evaluated. Microbiological inactivation and stability, rheological parameters, physical properties, volatile and fatty acid profiles, and bioactive compounds were assessed. TS provided more significant microbial inactivation (1 log CFU mL -1 ) and higher microbial stability during storage (21 days) than HTST, with 3, 2, and 2.8 log CFU mL -1 lower counts for yeasts and molds, aerobic mesophilic bacteria, and lactic acid bacteria, respectively. In addition, TS-treated samples showed higher anti-hypertensive (>39%), antioxidant (>33%), and anti-diabetic (>27%) activities, a higher concentration of phenolic compounds (>22%), preservation of anthocyanins, and better digestibility due to the smaller fat droplet size (observed by confocal laser scanning microscopy). Furthermore, lower TS powers (200 W) improved the fatty acid (higher monounsaturated and polyunsaturated fatty acid contents, 52.78 and 132.24%) and volatile (higher number of terpenes, n = 5) profiles and decreased the atherogenic index. On the other hand, higher TS powers (600 W) maintained the rheological parameters of the control product and contributed more significantly to the functional properties of the products (antioxidant, anti-hypertensive, and anti-diabetic). In conclusion, TS proved to be efficient in treating Jamun fruit dairy dessert, opening space for new studies to define process parameters and expand TS application in other food matrices., (Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.)

Ultrasound induced cavitation (acoustic cavitation) process is found useful in various applications. Scientists from various disciplines have been exploring the fundamental aspects of acoustic cavitation processes over several decades. It is well documented that extreme localised temperature and pressure conditions are generated when a cavitation bubble collapses. Several experimental techniques have also been developed to estimate cavitation bubble temperatures. Depending upon specific experimental conditions, light emission from cavitation bubbles is observed, referred to as sonoluminescence. Sonoluminescence studies have been used to develop a fundamental understanding of cavitation processes in single and multibubble systems. This minireview aims to provide some highlights on the development of basic understandings of acoustic cavitation processes using cavitation bubble temperature, sonoluminescence and interfacial chemistry over the past 2-3 decades., (Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.)

In this work, high purity graphite, a high chemically stable material, was effectively digested using a single method allowing compatible solutions for the further multitechnique determination of halogens by: ion chromatography (F and Cl), inductively coupled plasma mass spectrometry (Cl, Br and I) and by ion selective electrode (only for F). The recent system using microwave-induced self-ignition (MISI) is based on the strong interaction between microwave radiation and graphite in a closed system pressurized with oxygen (Maxwell-Wagner effect). Carbon-based materials present intense and specific interfacial polarization when exposed to microwave electromagnetic field resulting in a fast heating rate. This effect associated to a pressurized oxygen system, provides a quick self-ignition of carbon-based materials and consequent combustion/digestion of organic matrices. Under optimized conditions, sample masses up to 600 mg were fully digested in a quartz vessel under 20 bar of oxygen pressure and using just a diluted solution (100 mmol L -1  NH 4 OH) for the quantitative absorption of all the analytes. MISI method was validated, and the accuracy (better than 94%) was evaluated by comparison of results obtained by pyrohydrolysis for two coal certified reference materials as well as with subsequent analytes determination by the three techniques: IC, ICP-MS and ISE. It is important to point out that no filter paper disks, electrical connections or other ignition aids are required as in the case of previous or classical combustion methods. Moreover, just a diluted absorbing solution was used resulting in negligible blanks and relatively low limits of detection. The digestion efficiency was higher than 99%, making the proposed method a suitable and powerful alternative for the quasi complete digestion of graphite and determination of halogens virtually free of interferences., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

This study proposes the use of an ultrasound-assisted adsorption system coupled to porous ceramic fragments to improve the removal of iron from FeSO 4 aqueous solution. Ultrasound was applied using an ultrasound bath at a low frequency (37 kHz, 330 W). The optimized conditions for Fe removal were achieved by 7 g of adsorbent, 40 min of sonication, 20 mg L -1 of initial Fe concentration, and 30 °C of reaction temperature. After optimizing the conditions, the method was applied for the removal of iron in groundwater. A central composite design and response surface methodology were used to evaluate the degree to which different variables had a significant effect on iron removal. The efficiency of iron removal using the selected conditions for FeSO 4 solution was near to 100%. However, for groundwater samples, the maximum iron removal efficiencies of the system with and without ultrasound were 80.7% and 51.1%, respectively, indicating that the adsorption with ultrasound was significantly higher than that without ultrasound. It was shown that the proposed ultrasound-assisted adsorption system can be used to enhance the removal of inorganic iron from groundwater.

Many efforts have been recently made to improve the digestion efficiency by using powerful equipment or by using an auxiliary reagent. In this work, an alternative method is reported, which explores a digestion system based on a single reaction chamber (SRC) technology pressurized with O 2 for reducing the amount of acid, without impairing the digestion efficiency. Before digestion, the system was pressurized with compressed air (20 bar, 20% O 2 ) while the temperature was evaluated from 180 up to 270 °C. The procedure was also carried out under O 2 pressure (20 bar). For each temperature several acid concentrations were evaluated (0.1 to 3 mol L -1 HNO 3 ), being possible to correlate the effectiveness of each acid concentration with temperature. The proposed method was applied to the simultaneous digestion of several organic matrices with variable content of fat, protein, and carbohydrate (whole milk powder, bovine liver, parsley, and linseed). The residual carbon content was lower than 4% (C lower than 200 mg L -1 in digests), showing the high digestion efficiency of the proposed approach. Up to 250 mg of all food matrices were digested using a sub-stoichiometric amount of HNO 3 (1 mol L -1 solution), which was only achieved due to the use of O 2 as an auxiliary reagent. Barium, Ca, Cu, Fe, K, Mg, Mn, Na, Sr, and Zn were determined by ICP-OES, and the accuracy was better than 95% for standard reference materials of corn bran, whole milk powder, and bovine liver. It is an important feature, being in agreement with green chemistry recommendations because very low amounts of reagents are required for sample digestion, as well as low amounts of residues are generated.

This paper proposes a greener approach to the intensification of base oil recovery for truck engines (32,500 km of use) using ethanol, propan-2-ol, 2-methylpropan-1-ol, and butan-1-ol as solvents for the extraction of base oil, combining mechanical stirring (220 rpm) and ultrasound (25 °C, 24 kHz, and 400 W). The results indicated that the recovery yields of the base oil, using the mechanical stirring and ultrasound (MS-US) system, for ethanol, propan-2-ol, 2-methylpropan-1-ol, and butan-1-ol were approximately 3.1, 25.6, 71.6, and 85.5%, respectively. By contrast, the recovery yields using only mechanical stirring were 8.8, 28.9, 58.9, and 76.1%, respectively. The system with pre-extraction could effectively remove Ca (85.3-93.0%), Mg (67.2-82.9%), Na (31.7-62.5%), and Zn (0.0-71.7%). Finally, the results showed a reduction of almost 100% for the concentrations of Al, Cr, Fe, and Mo in the pre-extraction system. The mechanical stirring (5 min) and ultrasound (5 min) system were able to intensify the extraction process using environmentally friendly solvents., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Cultivars and fertilization levels influence rice productivity and can be associated with grain quality. Thus, it is possible to make decisions regarding the choice of cultivars and application of fertilizer levels based on the type of milling, a necessary post-harvest process that may minimize the nutrient load in the grains and result in loss in quality. This study relates the physicochemical composition and morphological quality of brown and polished milled rice grains, cultivar types, and different levels of soil fertilization using near-infrared spectroscopy analysis, X-ray diffraction and scanning electron microscopy. Statistical tools were used to test the various treatments and identify the relationship between factors and variables. A high fertilization level is related to increasing crude protein composition and starch for cultivar IRGA 431 CL associated with polished rice. However, the combination of cultivar IRGA 424 RI and brown rice demonstrated a higher grain resistance, and different percentages of whole, chalky, and damaged rice. The correlation between ash × crude protein and starch × crude fiber was found to be positive for brown rice and negative for the polished rice. Further, an increase in starch content was inversely proportional to the ash content, whereas an increase in crude protein was inversely proportional to the low-fat content in milled rice. The crystalline characteristics of rice starch were preserved at high fertilization levels associated with polished grains that demonstrated high starch content. Polished grains, however, showed more pores and cavities, and consequently greater permeabilities in the surface. It is recommended that batches of grains produced from cultivar IRGA 431 CL with high levels of fertilization be subjected to polished rice milling to achieve high protein and starch quality. However, grains from cultivar IRGA 424 RI with high levels of fertilization are recommended for brown rice milling owing to the high percentage of physical defects observed., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Microwave-induced combustion (MIC) system for further Cl, Br, and I determination in granola by inductively coupled plasma mass spectrometry (ICP-MS) was proposed. A high sample mass of granola was pressed as pellets and inserted into the proposed MIC system. Water and NH 4 OH were evaluated as absorbing solutions. The accuracy was estimated by the analysis of two certified reference materials and also by spike recoveries. Using the optimized conditions (zirconium ball milling, 1 g of granola and 6 mL of 50 mmol L -1 NH 4 OH), the agreement with the certified values ranged from 94 to 98% and recoveries higher than 95% were obtained. Low carbon concentration in digests (<25 mg L -1 ) was achieved, minimizing interferences by ICP-MS. Blanks were negligible and only diluted solutions were required. The concentration in samples ranged from 322 to 896, 0.618 to 0.980 and < 0.002 to 0.181 µg g -1 for Cl, Br and I, respectively., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Direct analysis of flour was proposed for the determination of Cl and S by an elemental analyzer for the first time. The main operational conditions of the direct solid sampling elemental analysis (DSS-EA) were optimized and calibrated by standard solutions, rather than by certified reference material (CRM). Accuracy was evaluated by the analysis of CRM of rice flour and by comparison with analyte determination by independent techniques, i.e., ion chromatography and inductively coupled plasma optical emission spectrometry; both were carried out after microwave-induced combustion. Sample mass from 0.5 to 260 mg was used and limits of quantification of 1.2 µg g -1 for Cl and 0.2 µg g -1 for S were achieved. Wheat, whole wheat, potato and corn flour were analyzed by DSS-EA. Concentrations of Cl and S ranged from 4.8 to 685 µg g -1 and from 13 to 1328 µg g -1 , respectively., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

In this work, two microwave-assisted sample preparation methods based on combustion and ultraviolet digestion for futher determination of Br, Cl, and I in blood by inductively coupled plasma mass spectrometry were proposed. For microwave-induced combustion (MIC), blood was spotted on square pieces of Munktell TFN paper, and water and diluted NH 4 OH were evaluated as absorbing solutions. The stability of the analytes in the spotted blood was also evaluated. During 90 days, no changes of halogen content were observed for samples (with and without anticoagulant) on paper stored in a desiccator at 20 ± 5 °C. Whole blood was also digested by microwave-assisted ultraviolet digestion (MAD-UV). Some parameters as the volume of HNO 3 solution, the influence of H 2 O 2 , and the effect of UV radiation were investigated. The interferences caused by C and K on halogen determination were carefully investigated. The results for MIC and MAD-UV and Br, Cl and I determination by ICP-MS presented no difference. The accuracy of MIC and MAD-UV was also evaluated by analyte spike with reference solutions of Br, Cl and I using inorganic species and also organic I standard (T4-levothyroxine). For both methods, recoveries were 94 and 108% for Br, 96 and 103% for Cl, and 104 and 97% for I. In case of organic I, recoveries were 99 and 111% for MIC and MAD-UV, respectively. The limits of quantification for MIC and MAD-UV after ICP-MS determination were 0.06 and 0.04 μg g -1 for Br, 14 and 30 μg g -1 for Cl and 12 and 8 ng g -1 for I, respectively. The proposed methods provide a suitable digestion approach, assuring safety and high digestion efficiency for further halogen determination in blood, with the possibility to use in clinical analysis., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Background: Seafood present important advantages for human nutrition, but it can also accumulate high levels of toxic and potentially toxic elements. Culinary treatments could influence seafood chemical element content and element bioavailability. In this study, the influence of culinary treatments on the total concentration and on the bioavailability of Cd, Cr, Cu and Pb in shark, shrimp, squid, oyster, and scallop was assessed., Methods: Boiling, frying, and sautéing with or without seasonings (salt, lemon juice and garlic) were evaluated. Total concentration and bioavailability of Cd, Cr, Cu and Pb in seafood after all these culinary treatments were compared with those in uncooked samples. Analytes were determined by triple-quadrupole inductively coupled plasma mass spectrometry (ICP-MS/MS). An alternative to express the results avoiding underestimated or overestimated values was proposed., Results: The analytes concentration in seafood without culinary treatment varied from 0.0030 μg g -1 (shrimp) to 0.338 μg g -1 (oyster) for Cd; 0.010 μg g -1 (squid) to 0.036 μg g -1 (oyster) for Cr; 0.088 μg g -1 (scallop) to 8.63 μg g -1 (oyster) for Cu, and < 0.005 μg g -1 (shrimp, squid and oyster) to 0.020 μg g -1 (shark) for Pb. Only Cd (in scallop) was influenced by culinary treatments (reduction from 37 to 53 % after boiling, frying, and sautéing). Bioavailability percentage varied from 11% (oyster) for Cd; 18% (oyster) to 41% (shark) for Cr; 6% (shark) for Cu, and 8% (oyster) for Pb. Bioavailability percentage was not influenced by culinary treatments., Conclusion: Cadmium concentration was reduced in scallop after some culinary treatments (reduction o 37-53% after boiling, frying, and sautéing), but bioavailability percentage was not influenced. The employed analytical method was adequate for the purpose, presenting import results for food safety assessment about the influence of culinary treatments on metals concentration and bioavailability in seafood., (Copyright © 2021 Elsevier GmbH. All rights reserved.)

In this work, ultrasound was applied for the conversion of tannic acid into gallic acid using only diluted H 2 O 2 as reagent. Experiments were carried out using several types of ultrasonic horns operating at 20 kHz (VC750W processor). The following experimental conditions were evaluated: H 2 O 2 concentration (0.2 to 8.5 mol L -1 ), horn type (10 to 25 mm of diameter), ultrasound amplitude (20 to 70%), sonication time (10 to 45 min), tannic acid concentration (170 to 1360 mg L -1 ), and reaction temperature (50 to 90 °C). Gallic acid production was monitored with ultra-performance liquid chromatography with high-resolution time-of-flight mass spectrometry (UPLC-ToF-MS). The isolated gallic acid was confirmed with nuclear magnetic resonance ( 1 H and 13 C NMR). It is important to emphasize that this study was developed as a proof of concept to demonstrate the potential of ultrasound for tannic acid conversion into gallic acid using just diluted H 2 O 2 . Under selected conditions gallic acid production yield was 128 ± 4 mg g -1 of initial tannic acid (using 170 mg L -1 of tannic acid as starting material). Reaction time was set as 30 min, which was carried out using 1 mol L -1 H 2 O 2 and ultrasound amplitude of 50% at 90 °C. At silent conditions (mechanical stirring, from 100 to 1000 rpm), gallic acid production was halved (less than 78 ± 4 mg g -1 of initial tannic acid)., (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)

Nowadays, the application of ultrasound (US) energy for assisting the lignocellulosic biomass and waste materials conversion into value-added products has dramatically increased. In this sense, this review covers theoretical aspects, promising applications, challenges and perspectives about US and its use for biomass treatment. The combination of US energy with a suitable reaction time, temperature and solvent contributes to the destruction of recalcitrant lignin structure, allowing the products to be used in thermochemical and biological process. The main mechanisms related to US propagation and impact on the fragmentation of lignocellulosic materials, selectivity, and yield of conversion treatments are discussed. Moreover, the synergistic effects between US and alternative green solvents with the perspective of industrial applications are investigated. The present survey analysed the last ten years of literature, studying challenges and perspectives of US application in biorefinery. We were aiming to highlight value-added products and some new areas of research., (Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.)

Nitrocellulose is a nitrated cellulose polymer with a broad application in industry. Depending on the nitrogen content, this polymer can be used for manufacturing explosives, varnishes, clothes, and films, being considered a product of high value-added. In this work, the use of ultrasound was investigated for the intensification of nitrocellulose synthesis from microcrystalline cellulose. The ultrasound-assisted nitrocellulose synthesis (UANS) was carried out using several ultrasound systems, such as baths and cup horns, allowing the evaluation of the frequency (from 20 to 130 kHz) and delivered power (from 23 to 134 W dm -3 ) to the reaction medium. The following parameters were evaluated: acid mixture (H 2 SO 4 , H 3 PO 4 , CH 2 O 2 or CH 3 COOH with HNO 3 , 2 to 14.4 mol L -1 ), ultrasound amplitude (10 to 70%) and reaction time (5 to 50 min). Better nitrocellulose yield (nitrogen content of 12.5% was obtained from 1 g of microcrystalline cellulose employing a cup horn system operating at 20 kHz, 750 W of nominal power with 60% of amplitude, 25 mL of acid solution (13.6 mL of 18.4 mol L -1 H 2 SO 4  + 9.2 mL of 14.4 mol L -1 HNO 3  + 2.2 mL H 2 O), at 30 °C for 30 min. At silent conditions (mechanical stirring ranging from 100 to 500 rpm), the nitrogen content was lower than 11.8% which demonstrate the ultrasound effects for nitrocellulose synthesis., (Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.)

Urban dust is a mixture of deposited particles from different sources usually linked to potentially toxic elements (PTEs). Despite the industrialization of many South American countries, little is known about the impact of particulate matter in large cities; these data are necessary to promote environmental policies aiming to protect human health. The main objective of this work was to evaluate the particle size distribution, composition, and environmental and human health risks of settled dust particles from Barranquilla, a Colombian Caribbean industrialized area. Trace elements were analyzed by inductively coupled plasma-mass spectrometry from 35 different sites, covering all city areas. Dust was mostly composed of 10-to-70-μm particles. The average concentrations of V, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Mo, Ag, Cd, Sn, Sb, Pb, and Bi were above background. High spatial heterogeneity was observed for Cu, Zn, As, Se, Mo, Ag, Sn, Sb, and Bi. Concentration factors suggest that urban dusts are extremely contaminated by Zn and Cu. The ecological risk associated with specific elements decreased in the order Cd > Cu > As > Hg > Pb > Ni > Co ≈ Zn ≈ Cr, and the contamination load index showed that 91% of the samples are polluted by PTEs. Although the carcinogenic risks of Cr, Ni, As, Co, and Cd were low, chronic exposure to several PTEs may affect quality of life. Educational programs, as well as monitoring and greater control on traffic, industry, and construction activities are needed to protect environmental and human health., (© 2021 The Authors. Journal of Environmental Quality © 2021 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.)

Good air quality is documented as a significant factor of social justice. The human health hazards associated with air pollution are not distributed equally across cities; the most vulnerable people are more exposed to ambient air as they commute to work and wait for buses or trains at the stations. Aerosols play important roles in atmosphere quality and the climate; their oxidation at the nanoscale level may possibly increase the reactivity and toxicity of atmospheric particulates. Indoor school environments are characterized by high concentrations of different airborne particulate and gaseous pollutants. The documentation of nanoparticles (NPs), ultra-fine particles (UFPs), and micron-size particle species present in indoor primary schools are an important aspect in the recognition of their influence in respirational difficulties and decreased cognitive progress in children. This work utilizes the study of condensed water, sampled with portable dehumidifiers (PD), to describe NPs and UFPs in the vapor stage of enclosed zones. The acquired extracts were analyzed by advanced electron microscopy techniques. A total of 392 NPs and 251 UFPs were examined in a set of 22 samples acquired in moderately limited or inadequately ventilated indoor areas from several schools. Noting that NPs-related disorders happen at particular places of respirational structure, identification of site-specific NPs accumulation should be anticipated in direction to better verify the corresponding human health outcomes resulting from respirable NPs., Competing Interests: Declaration of competing interest The authors have not conflict of interest., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

A method for the determination of microplastic (MP) content in seafood is proposed based on the selective digestion of seafood without the degradation of MP. A simple approach was developed using diluted acid with microwave-assisted wet digestion. The following parameters were evaluated: nitric acid concentration (0.5 to 14.4 mol L -1 ), digestion temperature (180 to 220 °C), irradiation program holding time (10 to 30 min), MP particle size (0.3 to 5 mm), and the seafood mass (0.5 to 2 g). To develop a reliable method for the determination of MP amount, up to 2 g of an in natura seafood sample were spiked with a known amount of MP (100 mg of mixed MP). Suitable conditions were obtained using 1 mol L -1 HNO 3 at 200 °C (10 min holding time). Digests were filtered and the plastic content was gravimetrically determined. The heating program was 20 min, which represents a significant reduction in the time normally reported in the literature for MP analysis (from few hours up to 3 days). The proposed method allowed gravimetric determination of eight plastic types (polyethylene terephthalate, polystyrene, expanded polystyrene, polypropylene, high and low density polyethylene, polycarbonate and polyvinyl chloride) with particle size ≥0.3 mm. Up to 2 g of an in natura seafood sample (shark species, acoupa weakfish, tuna fish, trahira, and pink shrimp) were efficiently digested, which opened the possibility of using the proposed digestion method for determining elemental contaminants (Al, As, Ca, Cd, Co, Cr, Cu, Fe, Hg, La, Mg, Mn, Mo, Ni, Pb, and Zn). Thus, as the main feature of the proposed digestion method is the possibility of determining MP and elemental contaminants using the same digestion protocol, saves time and reagents and provides accurate and precise information about different classes of marine pollutants (MP and elemental contaminants)., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

In this work, the in-tip thermal infrared enthalpimetry (in-tip TIE) method is proposed for fast enthalpimetric analysis. In this method, the reactions inside the tips of a multichannel pipette were combined with temperature monitoring by an infrared camera. The filter paper was used inside the tips to retain reagents as solutions (wetted paper mode) or as solids (dried paper mode) to perform neutralization, redox, or precipitation reactions. The dried reagents inside the tips were obtained by oven drying a solution retained in the filter paper. The determination of the total acidity of the vinegar, ascorbic acid in vitamin C tablets, and chloride in soy sauces and saline inhalation solutions was performed as examples of the application of the proposed method. The agreement with reference methods ranged from 98 to 107%. The use of reagents dried inside the tip was feasible, leading to a simple aspiration of sample solution within the 12 tips of the pipette to perform a rapid analysis (1 min). Therefore, up to 720 measurements in 1 h were feasible for in-tip TIE over up to 12 measurements for the reference methods. Moreover, miniaturization reduced reagent consumption and residue generation. For example, for in-tip TIE, only 3.6 mL of residues was generated ( n = 12) over 60-240 mL in reference methods ( n = 3). Contrarily to other TIE methods, no microplates or stirring was required, opening possibilities for field analysis since the multichannel pipette and the infrared camera are both operated with batteries.

The exposition of mate (Ilex Paraguariensis A. St.-Hil.) to As and Cd was investigated in plants derived from young mini-cuttings. Mate plants were cultivated in a closed soilless system, composed of coarse sand as substrate and flood fertirrigation. Plantlets were fertirrigated with nutritive solution and As and Cd solutions were added to the nutritive solution in the final concentration of 8 and 17 mg L -1 (As) and of 17 and 33 mg L -1 (Cd) during 14 days. Results show that stem diameter and Dickson quality index (DQI) variables could not be used as a potential indicator of accumulation of As and Cd. The shoot height, number of leaves and chlorophyll index are variables easy and quick to measure and they can be used as parameters to evaluate the stress caused in mate plants cultivation in a closed soilless system. The highest concentration of As and Cd was in roots of plants. Beyond the roots, As and Cd also can be translocated to the leaves achieving high concentrations. In addition, leaves from the treated mate plants were submitted to a hot infusion extraction in order to simulate the traditional beverage and As and Cd were determined in the infusion. Regarding to the infusion procedure, considerable As and Cd amounts were extracted from the leaves leading to conclude that this way of consumption can be an important source of toxic elements for the human diet., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

A new procedure is proposed for the determination of metal contaminants (Cr, Fe, Mg, Mn, Na and Ni) in polymeric diphenylmethane diisocyanate (PMDI), a raw material used to produce polyurethane polymers. The feasibility of using Zeeman-effect background correction graphite furnace atomic absorption spectrometry (GF AAS) and direct sampling (DS) was evaluated. Calibration using aqueous standard solutions was feasible and chemical modifiers as well as reference materials for this purpose were unnecessary. Relatively high sample masses (up to 23 mg) were used allowing very low limits of detection ranging from 0.06 ng g -1 to 1 ng g -1 (Fe and Mg) up to 3  ng g -1 (Ni), with relative standard deviation lower than 15%. The following parameters were evaluated: pyrolysis and atomization temperatures , sample mass, as well as the use of low sensitivity conditions (Zeeman effect background correction magnetic field strength adjustment and the use of a secondary wavelength for Fe and Na determinations, respectively). Results were compared with those obtained by microwave-assisted digestion and microwave-induced combustion with subsequent analytes determination by inductively coupled plasma optical emission spectrometry (ICP-OES) and by inductively coupled plasma mass spectrometry (ICP-MS). No significant difference was observed between the results obtained by DS-GF AAS, ICP-MS and ICP-OES after both digestion systems. The proposed DS-GF AAS method allowed the determination of six elements in PMDI (which is considered as a complex matrix) with limits of detection lower than those achieved by other methods. This new procedure can be used as quality control of polyurethanes industry for ultra-trace inorganic impurities., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Rationale: A method for the determination of rare earth elements in carbonatite rocks by inductively coupled plasma mass spectrometry (ICP-MS) was developed., Methods: An alkaline rock, carbonatite, was submitted to ultrasound-assisted extraction (USAE) using an ultrasound bath, a cup horn system or an ultrasound probe. The main USAE parameters were evaluated, such as extraction time (1 to 30 min), extraction temperature (20 to 100°C) and ultrasound amplitude (10 to 100%). For ICP-MS, a desolvation system (APEX-Q) was used to reduce interference in lanthanide determination. To evaluate if the effect of ultrasound improved extraction, experiments were carried out using magnetic stirring (500 rpm) for comparison., Results: The temperature and ultrasound amplitude optimized for the method were 70°C and 40%, respectively, using dilute nitric acid (3% v/v). Quantitative analyte recoveries were obtained using an ultrasound bath (25 kHz/100 W) which allowed for the simultaneous extraction of twelve replicates., Conclusions: All the results obtained with the use of ultrasound systems were better than those obtained with mechanical stirring. The extracts were suitable for ICP-MS analysis and the results were in agreement with those obtained by the reference method (using wet acid digestion). Based on the results, the use of USAE can be considered an alternative method for sample preparation of carbonatite rocks, under milder conditions, for further ICP-MS analysis., (© 2020 John Wiley & Sons, Ltd.)

Rationale: Bromine and iodine have important physiological functions; however, in inadequate concentration, they can also cause several physiological problems. Their mobility assessment in human organisms through biological sampling may help clarify some doubts related to metabolic routes, which are still not well elucidated. In this context, a suitable analytical method for this purpose should be developed., Methods: An analytical method for determining ultratrace levels of bromine and iodine in human nail samples was developed. Inductively coupled plasma mass spectrometry (ICP-MS) using a conventional nebulization system was immediately chosen as the determination tool because of its powerful sensitivity and selectivity. Sample preparation methods including microwave-induced combustion (MIC), microwave-assisted extraction, and microwave-assisted digestion were evaluated. The compatibility of the final solutions with ICP-MS analysis was considered while the method was developed., Results: MIC was chosen as the most suitable method for the sample preparation for determining the levels of bromine and iodine in human nail samples using ICP-MS. Unlike other sample preparation methods, this one fully eliminated interferences related to the carbon content and memory effects. Sample masses up to 100 mg were efficiently digested, and the analytes were quantitatively absorbed using only 50 mmol L -1 NH 4 OH solution. Recoveries ranged from 93% to 102%, and the relative standard deviation was < 8%., Conclusions: The proposed analytical method presents important characteristics for routine analysis. It allows ultratrace determination even when low sample masses are used because of the low blank values, reduced volume of reagents, and powerful detectability using ICP-MS., (© 2019 John Wiley & Sons, Ltd.)

Rationale: In this review, works published in the past 25 years for fluorine, chlorine, bromine, and iodine determination in several matrices by inductively coupled plasma mass spectrometry (ICP-MS) were covered. Usually, the determination of halogens has been performed by ICP-MS using a previous sample preparation step or, more recently, by direct analysis of solid or liquid samples., Methods: Methods based on combustion, extraction, pyrohydrolysis, sample dilution in organic or aqueous medium, and wet digestion, among others, are discussed. Moreover, the recent applications of methods based on laser ablation (LA) and electrothermal vaporization (ETV) coupled to ICP-MS are discussed., Results: The main challenge for methods using sample preparation has been to obtain a final solution compatible with ICP-MS, as well as to overcome problems related to analyte losses and contamination. Interferences due to the presence of dissolved organic compounds in solution, enhancement or suppression of ionization of analytes, and related matrix effects have been of concern when using ICP-MS. For the determination of halogens by ICP-MS using LA and ETV systems, some limitations related to the difficulty of calibration are pointed out, impairing the widespread use of this approach., Conclusions: A critical view is presented for further halogen determination by ICP-MS, mainly for matrices considered difficult to digest using conventional protocols., (© 2020 John Wiley & Sons, Ltd.)

The development of natural additives is considered an important research topic. In this work, the use of Cymbopogon citratus (CC) extract as a natural additive for chicken sausage refrigerated was investigated. The CC extract was characterized by electrospray ionization with high-resolution time-of-flight mass spectrometry (ESI-ToF-MS) and the identified compounds were directly related to the antioxidant activity demonstrated by CC in the fresh sausage. In total, 31 phytochemical compounds were identified, and 27 of these still were not described in the literature for CC. The antimicrobial activity showed that CC extract is a potential antibacterial agent. Besides, the results showed that CC extract reduced lipid oxidation compared to synthetic additive. The sensorial characteristics were maintained, demonstrating good acceptability by the consumer. The results confirmed that CC can keep the quality of chicken sausage refrigerated for up to 42 days of storage., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

A novel system for sample digestion was proposed based on microwave-induced combustion in disposable vessels (MIC-DV) for trace elements determination by inductively coupled plasma optical emission spectrometry (ICP-OES). As a proof of concept, botanical samples were digested by MIC-DV for further determination of Al, Cr, Cu, Fe, Mn, Sr, and Zn. The system consists of a quartz holder (a modified version of conventional MIC) placed inside disposable polypropylene (PP) vessels. The quartz holder was carefully designed to avoid excessive heating and damaging of the vessel walls. For the combustion, the PP vessels containing the quartz holder and sample were placed in a specially designed metallic rotor that prevents the heating of absorbing solution and allows the use of a domestic microwave oven for sample ignition. After combustion, the digestion vessel was shaken to ensure the analytes' absorption. The single-vessel principle was fulfilled, since no further dilution or liquid transfer was required and the same PP vessel used for digestion allowed solution storage until element determination. The influences of absorbing solution (diluted HNO 3 and water) and sample mass (10 to 30 mg) were evaluated. By using the proposed MIC-DV system, low volumes of diluted absorbing solutions (5 mL of 1 mol L -1 HNO 3 ) were possible, allowing the use of low reagent amounts and low energy consumption, since microwave irradiation is used only for sample ignition. The agreement with certified values ranged from 92 to 108% for all analytes, whereas the precision was below 15%. All of these advantages, combined with the use of low-cost disposable vessels and instrumentation, make MIC-DV suitable to be used for research and routine analysis.

The tannery industry generates huge amount of waste with high Cr concentration, being classified as a dangerous waste. The development of alternative treatments for these residues aiming environmental friendly protocols are important topics of research. In this work, the use of ultrasound (US) energy for Cr removal from residual tanned leather was investigated. Ultrasound-assisted extraction (UAE) experiments were carried out in several systems as ultrasonic baths, cup horns, and probes, allowing to evaluate several frequencies (20-130 kHz) and power delivered to the extraction system. The following experimental conditions were evaluated: extraction solution (HCl, HNO 3 , H 2 SO 4 , CH 2 O 2 and C 2 H 2 O 4 ), temperature (10-90 °C), time (1-40 min), US amplitude (10-90%), feedstock amount (50-450 mg), and concentration of extraction solution (0.1-4 mol L -1 ). A multivariate factorial design with 10 axial points and 3 central points was applied. After UAE optimization an efficiency of 92% was achieved for Cr removal using 150 mg of feedstock, 3 mol L -1 HNO 3 , at 30 °C, 90% of amplitude, and 30 min. The same efficiency was not observed using mechanical stirring (100-500 rpm), which was lower than 65%. To prove the applicability of the proposed process some experiments for scaling up were performed using several reactor loads (1-9 L). Moreover, using the proposed UAE process Cr was efficiently removed at lower reaction time and at room temperature only by using US and diluted acid solution, representing energy and reagents saving., (Copyright © 2019 Elsevier B.V. All rights reserved.)

The aim of this study was to investigate the influence of hi-maize, inulin, and rice bran in the survival of Lactobacillus acidophilus LA-5 in pectin microparticles obtained by internal gelation and subjected to freeze-drying. For this, the development of a matrix capable of extending Lactobacillus acidophilus viability to develop new functional foods was emphasized. Microparticle size, encapsulation efficiency, probiotic survivability after gastrointestinal simulation, and storage stability were analyzed. The pectin + inulin encapsulation matrix presented the highest encapsulation efficiency (68.1%) compared to the other treatments. Microparticle sizes ranged from 166 ± 2 µm (pectin + hi-maize) to 345 ± 9 µm (pectin + inulin). The microparticles added from the different prebiotics showed better microorganism protection when compared to treatment without prebiotics, which presented greater viability in the gastrointestinal simulation. Under storage conditions of 25 °C and -18 °C, the microparticles containing hi-maize, inulin, and rice bran maintained the probiotic microorganisms viable for longer periods than the pectin microparticles. At 7 °C, the pectin + rice bran treatment stood out from the other treatments, as it was able to maintain probiotic stability during 120 days of storage., (Copyright © 2019 Elsevier Ltd. All rights reserved.)