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11. Neospora caninum and Toxoplasma gondii: Relationship between hepatic lesions, cytological and biochemical analysis of the cavitary liquid during the acute phase of the diseases in experimental models

Author

Bottari, Nathieli B., Tonin, Alexandre A., Fighera, Rafael, Flores, Mariana M., França, Raqueli T., Camillo, Giovana, Toscan, Gustavo, Vogel, Fernanda S.F., Sangoi, Manuela B., Bochi, Guilherme V., Moresco, Rafael N., Lopes, Sonia T.A., and Da Silva, Aleksandro S.

Published
2014
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13. Pathology in Practice

Author

de Aguiar, Ariel, primary, Amaral, Bruna P., additional, Barraza, Vanessa C. T., additional, da Silva, Tanara Raquel O., additional, Simon, Veridiana, additional, Vogel, Fernanda S. F., additional, and Flores, Mariana M., additional

Published
2022
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15. Evidence of Helicobacter spp. in Saliva and Gastric Mucosa of Domestic Dogs in the Central Region of Rio Grande do Sul, Brazil

Author

Guerra Segundo, Daniel D., primary, Mello, Camila B. E., additional, Cargnelutti, Juliana F., additional, Flores, Mariana M., additional, Pedrotti, Luís F., additional, Antunes, Bernardo N., additional, Milech, Vanessa, additional, Velasquez, Omar G., additional, Martins, Letícia R., additional, and Pinto Filho, Saulo T. L., additional

Published
2021
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16. What Is Your Diagnosis?

Author

Beck, Antônio A., primary, De La Côrte, Flávio D., additional, Silva, Gabriele B., additional, Frank, Maria I., additional, Flores, Mariana M., additional, and Santos, Alex, additional

Published
2020
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21. Detection of Equus caballuspapillomavirus-2 in equine penile/preputial papillomas and squamous cell carcinomas in southern Brazil

Author

da Silva, Tanara Raquel de Oliveira, Gonçalves, Paula Nitiana Campos, Marcus, Valentina Berté, Mucellini, Carolina Isabela, dos Santos, Igor Ribeiro, Kommers, Glaucia, Driemeier, David, Flores, Eduardo Furtado, Cargnelutti, Juliana Felipetto, and Flores, Mariana M.

Abstract

For approximately one decade, a novel papillomavirus termed Equus caballuspapillomavirus-2 (EcPV-2) has been associated with equine penile/preputial papillomas and squamous cell carcinomas (SCCs). It is currently believed that the virus has a carcinogenic activity, being able to induce such neoplastic lesions. After being first described, EcPV-2 has been detected in many countries from North America, Europe, and Asia; however, to date, it has not been reported in Brazil. The aim of this research was to investigate the presence of EcPV-2 in penile/preputial papillomas and SCCs of Brazilian horses. Forty samples diagnosed as equine penile and/or preputial papillomas, carcinomas in situ (CIS), or SCCs in two veterinary anatomic pathology services from southern Brazil were investigated. Histologic evaluation and immunohistochemistry (IHC) using a BPV-1 antibody were performed. Posteriorly, the samples were submitted to polymerase chain reaction using two broad-spectrum (MY09/11 and FAP) and one EcPV-2-specific primer sets. Positive samples were sequenced. PV antigen expression was detected in one papilloma, one CIS, and two SCCs by IHC. Five SCCs, one papilloma, and one CIS were PV-positive on PCR. Sequencing of the seven PCR products revealed homology with EcPV-2. This study confirms the occurrence of EcPV-2 infection in Brazilian horses. Moreover, the results presented here provide useful information concerning the phylogeny from the viruses detected in our samples. We hope to encourage further studies on this novel agent, contributing to its characterization, and, possibly, to the eventual development of preventive measurements, including a possible vaccine.

Published
2022
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25. Pathology in Practice

Author

Hodgson, Michelle M., primary, Grimes, Janet A., additional, Boudreaux, Bonnie B., additional, Flores, Mariana M., additional, and Langohr, Ingeborg M., additional

Published
2018
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27. Aspectos epidemiológicos, clínicos e anatomopatológicos da intoxicação por aceturato de diminazeno em cães

Author

Flores, Mariana M., Pereira, Paula R., Mazzanti, Alexandre, Kommers, Glaucia D., and Fighera, Rafael A.

Subjects
diminazene aceturate, Doenças de cães, Diseases of dogs, toxicosis, terapia antiprotozoário, antiprotozoal therapy, aceturato de diminazeno, toxicose, toxicologia, toxicology
Abstract

Os aspectos epidemiológicos, clínicos e anatomopatológicos da intoxicação espontânea por aceturato de diminazeno foram estudados em 10 cães. Em todos os casos, os cães afetados demonstraram sinais de síndrome tálamo-cortical, principalmente alteração do nível de consciência, tetraparesia, rigidez extensora e crise convulsiva. Em alguns casos, os cães acometidos apresentaram sinais de síndrome cerebelar, como tremores musculares generalizados de alta frequência e baixa amplitude, e/ou de síndrome vestibular, como ataxia, inclinação de cabeça e quedas. Esses sinais ocorreram entre 24 e 48 horas após o uso do fármaco injetável por via intramuscular e se mantiveram até a morte ou eutanásia dos cães (entre 1 e 7 dias). Tais sinais clínicos refletiam encefalomalacia hemorrágica focal simétrica, que afetava a medula oblonga, a ponte, a medular do cerebelo, o tálamo, o mesencéfalo, os pedúnculos cerebelares e os núcleos da base. Esse artigo: 1) descreve e discute essa forma de intoxicação medicamentosa tão pouco citada na literatura internacional e desconhecida da maior parte dos clínicos e patologistas veterinários brasileiros, 2) estabelece critérios clínicos e anatomopatológicos para o seu diagnóstico e, principalmente, 3) atenta para os riscos da utilização desse princípio ativo na terapêutica canina. The epidemiological, clinical, and pathological aspects of diminazene aceturate (DA) spontaneous toxicosis were evaluated in 10 dogs. All affected dogs developed signs of thalamic-cortical syndrome, characterized mainly by neurological changes in the conscience levels, tetraparesis, extensor stiffness, and seizures. In some cases there was also evidence of cerebellar syndrome, characterized by generalized muscle tremors (high-frequency and low-amplitude) and/or vestibular syndrome, characterized by or ataxia, head tilt, and falling. These clinical signs occurred between 24 and 48 hours following intramuscular administration of DA and persisted until spontaneous death or euthanasia occurred between 1 and 7 days after the onset of clinical signs. The mentioned clinical signs reflected lesions that consisted of focal symmetrical hemorrhagic encephalomalacia affecting medulla oblongata, pons, cerebellar medulla, thalamus, midbrain, cerebellar peduncles, and basal nuclei. This article (1) describes and discusses DA toxicosis in dogs, a poorly-described clinical entity that is unknown by most clinicians and pathologists in Brazil; (2) establishes the clinical and pathological criteria for the diagnosis of DA toxicosis in dogs; and (3) calls up the attention for the risks of using DA in dogs in clinical settings.

Published
2014

28. Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo

Author

Zanette, Régis A., primary, Bitencourt, Paula E. R., additional, Kontoyiannis, Dimitrios P., additional, Fighera, Rafael A., additional, Flores, Mariana M., additional, Kommers, Glaucia D., additional, Silva, Priscila S., additional, Ludwig, Aline, additional, Moretto, Maria B., additional, Alves, Sydney H., additional, and Santurio, Janio M., additional

Published
2015
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29. E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans

Author

Azevedo, Maria Isabel de, primary, Ferreiro, Laerte, additional, Da Silva, Aleksandro S., additional, Tonin, Alexandre A., additional, Ruchel, Jader B., additional, Rezer, João F.P., additional, França, Raqueli T., additional, Zimmermann, Carine E.P., additional, Leal, Daniela B.R., additional, Duarte, Marta M.M.F., additional, Lopes, Sonia T.A., additional, Flores, Mariana M., additional, Fighera, Rafael, additional, and Santurio, Janio M., additional

Published
2014
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30. Influence of toxoplasmosis on acetylcholinesterase activity, nitric oxide levels and cellular lesion on the brain of mice

Author

Tonin, Alexandre A., primary, Da Silva, Aleksandro S., additional, Thomé, Gustavo R., additional, Sangoi, Manuela B., additional, Oliveira, Lizielle S., additional, Flores, Mariana M., additional, Schetinger, Maria Rosa C., additional, Fighera, Rafael A., additional, Moresco, Rafael N., additional, Camillo, Giovana, additional, Vogel, Fernanda S.F., additional, and Lopes, Sonia T.A., additional

Published
2014
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33. Relationship between butyrylcholinesterase activity and liver injury in mice acute infected with Toxoplasma gondii

Author

Da Silva, Aleksandro S., primary, Tonin, Alexandre A., additional, Thorstenberg, Maria L., additional, Leal, Daniela B.R., additional, Fighera, Rafael, additional, Flores, Mariana M., additional, França, Raqueli T., additional, Camillo, Giovana, additional, Vogel, Fernanda S.F., additional, Rue, Mario de la, additional, and Lopes, Sonia T.A., additional

Published
2013
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40. E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans.

Author

de Azevedo MI, Ferreiro L, Da Silva AS, Tonin AA, Ruchel JB, Rezer JF, França RT, Zimmermann CE, Leal DB, Duarte MM, Lopes ST, Flores MM, Fighera R, and Santurio JM

Subjects
Animals, Cryptococcosis immunology, Cytokines blood, Histological Techniques, Leukocyte Count, Lymphocytes metabolism, Rats, Time Factors, Adenosine Deaminase metabolism, Antigens, CD metabolism, Apyrase metabolism, Cryptococcosis enzymology, Cryptococcus neoformans immunology
Abstract

Cryptococcus neoformans, the etiological agent of cryptococcosis, is an opportunistic fungal pathogen of immunocompromised individuals. The aim of this study was to evaluate the activities of E-NTPDase and E-ADA in rats experimentally infected by C. neoformans var. grubii. Adult rats (35) were divided in two groups: 18 for the control group (uninfected) (A), and 17 for the infected group (B). Each group was separated into three sub-groups (A1, A2, A3-B1, B2, B3), and samples were collected on 10, 20, and 30 days post-infection (PI). Leukocyte counts, IFN-γ, TNF-α, IgM, IgG levels, and E-NTPDase and E-ADA activities were analyzed. It was possible to observe that IgG and IgM seric levels of infected rats were significantly elevated (P<0.01) on days 10, 20 and 30 PI, as well as the levels of TNF-α and INF-γ when compared to uninfected rodents. Regarding E-NTPDase activity in lymphocytes, it was possible to observe that the ATP hydrolysis was significantly decreased on days 20 (P<0.01) and 30 PI (P<0.05), while ADP hydrolysis was significantly reduced only on day 20 PI (P<0.01) when compared with uninfected group. Seric E-ADA activity had a significant reduction (P<0.01) during all three evaluated periods when compared to the control group, while E-ADA activity in lymphocytes increased significantly (P<0.01) when compared to the group A on day 10 PI; however on days 20 and 30 PI, its activity was considerable reduced in lymphocytes of infected animals (P<0.01). Therefore, it is possible to conclude that the infection caused by C. neoformans in immunocompetent rats leads to changes in the purinergic signaling (NTPDase and E-ADA), concomitantly with an inflammatory response (increased levels of cytokines and immunoglobulins) associated with inflammatory infiltrates and histological lesions in the lung., (Copyright © 2014 Elsevier B.V. All rights reserved.)

Published
2014
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41. Influence of toxoplasmosis on acetylcholinesterase activity, nitric oxide levels and cellular lesion on the brain of mice.

Author

Tonin AA, Da Silva AS, Thomé GR, Sangoi MB, Oliveira LS, Flores MM, Schetinger MR, Fighera RA, Moresco RN, Camillo G, Vogel FS, and Lopes ST

Subjects
Animals, Brain pathology, Male, Mice, Oxidative Stress, Toxoplasma, Toxoplasmosis, Animal pathology, Toxoplasmosis, Cerebral pathology, Acetylcholinesterase metabolism, Brain metabolism, Nitric Oxide metabolism, Toxoplasmosis, Animal metabolism, Toxoplasmosis, Cerebral metabolism
Abstract

The objective of this study was to investigate the activity of acetylcholinesterase (AChE), nitrite/nitrate (NOx) levels, as well as the biomarkers of cellular damage in the brain of mice experimentally infected with Toxoplasma gondii. Sixty mice were divided into two experiments: in experiment I the mice were infected with T. gondii/RH strain, while in experiment II they were infected with T. gondii, strains VEG and ME-49. Our evaluations were carried out on brain homogenized samples, assessing the AChE and glutathione reductase (GR) activities, and NOx, TBARS and AOPP levels in all the infected animals, compared with the control group. In both experiments, I and II, it was observed an increase in the activity of AChE and GR, as well as in the levels of NOx in the brain of infected mice with T. gondii. TBARS levels were increased in mice infected with the three different strains, RH, ME-49, and VEG. AOPP concentration was increased only in mice infected with the RH strain. Animals infected with the strains VEG and ME-49 showed histological lesions, associated with the presence of the parasite in the brain. Therefore, the infection by T. gondii is able to interfere in cholinesterase activity and NO levels, in association with oxidative stress and histological lesion., (Copyright © 2014 Elsevier GmbH. All rights reserved.)

Published
2014
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42. Relationship between butyrylcholinesterase activity and liver injury in mice acute infected with Toxoplasma gondii.

Author

Da Silva AS, Tonin AA, Thorstenberg ML, Leal DB, Fighera R, Flores MM, França RT, Camillo G, Vogel FS, de la Rue M, and Lopes ST

Subjects
Animals, Brain enzymology, Brain pathology, Butyrylcholinesterase analysis, Liver enzymology, Liver pathology, Liver Diseases, Parasitic pathology, Male, Mice, Mice, Inbred BALB C, Toxoplasmosis, Animal pathology, Butyrylcholinesterase metabolism, Liver Diseases, Parasitic enzymology, Toxoplasmosis, Animal enzymology
Abstract

This study aimed to investigate the butyrylcholinesterase (BChE) activity in mice experimentally infected with Toxoplasma gondii during the acute phase. Twenty mice were divided in two groups with 10 animals each: group A was composed of uninfected mice while group B was formed by rodents infected with T. gondii. Five days after infection, blood was collected and serum separated, and fragments of liver and brain were obtained. In serum and liver homogenate was noted a significant reduction (P<0.05) in BChE activity in infected mice when compared with uninfected ones. In serum was observed an increase in the activity of alanine aminotransferase and urea, associated with reduction in alkaline phosphatase activity and in the levels of total protein and albumin. Histologically, there were foci of necrosis and parasites in the forms of tachyzoites and cysts, with bradyzoites in liver samples of infected animals. Based on these results, we conclude that toxoplasmosis reduces BChE activity in mice, and this alteration is probably related to the liver damage caused by the parasitism. Therefore, this enzymatic alteration can directly contribute to the pathogenesis of the disease., (Copyright © 2012 Elsevier GmbH. All rights reserved.)

Published
2013
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