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13 results on '"Floerchinger, Alessia"'

3. Optimizing metastatic-cascade-dependent Rac1 targeting in breast cancer: Guidance using optical window intravital FRET imaging

Author

Floerchinger, Alessia, Murphy, Kendelle J., Latham, Sharissa L., Warren, Sean C., McCulloch, Andrew T., Lee, Young-Kyung, Stoehr, Janett, Mélénec, Pauline, Guaman, Cris S., Metcalf, Xanthe L., Lee, Victoria, Zaratzian, Anaiis, Da Silva, Andrew, Tayao, Michael, Rolo, Sonia, Phimmachanh, Monica, Sultani, Ghazal, McDonald, Laura, Mason, Susan M., Ferrari, Nicola, Ooms, Lisa M., Johnsson, Anna-Karin E., Spence, Heather J., Olson, Michael F., Machesky, Laura M., Sansom, Owen J., Morton, Jennifer P., Mitchell, Christina A., Samuel, Michael S., Croucher, David R., Welch, Heidi C.E., Blyth, Karen, Caldon, C. Elizabeth, Herrmann, David, Anderson, Kurt I., Timpson, Paul, and Nobis, Max

Published
2021
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5. Integrative multi-omics identifies regulatory and exhausted T cell types and novel immunotherapy targets in CLL lymph nodes

Author

Seiffert, Martina, primary, Cid, Laura Llaó, additional, Wong, John, additional, Floerchinger, Alessia, additional, Paul, Yashna, additional, Schifflers, Christoph, additional, Mallm, Jan-Philipp, additional, Lichter, Peter, additional, Iskar, Murat, additional, Zapatka, Marc, additional, Moussay, Etienne, additional, Paggetti, Jérôme, additional, Botana, Iria Fernandez, additional, Wierz, Marina, additional, Pagano, Giulia, additional, Gonder, Susanne, additional, Cosma, Antonio, additional, Chazotte, Margot, additional, Bestak, Kresimir, additional, Schapiro, Denis, additional, Roider, Tobias, additional, Czernilofsky, Felix, additional, Bruch, Peter-Martin, additional, Dietrich, Sascha, additional, Campton, D, additional, Gerhard-Hartmann, Elena, additional, Rosenwald, Andreas, additional, Colomer, Dolors, additional, and Campo, Elias, additional

Published
2024
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9. Optimizing metastatic-cascade-dependent Rac1 targeting in breast cancer: Guidance using optical window intravital FRET imaging

Author

Floerchinger, Alessia, primary, Murphy, Kendelle J, primary, Latham, Sharissa L., primary, C.Warren, Sean, primary, McCulloch, Andrew T, primary, Kyung Lee, Young, primary, Stoehr, Janett, primary, Mélénec, Pauline, primary, S.Guaman, Cris, primary, Metcalf, Xanthe L, primary, Lee, Victoria, primary, Zaratzian, Anaiis, primary, Da Silva, Andrew, primary, Tayao, Michael, primary, Rolo, Sonia, primary, Phimmachanh, Monica, primary, Sultani, Ghazal, primary, McDonald, Laura, primary, M. Mason, Susan, primary, Ferrari, Nicola, primary, M. Ooms, Lisa, primary, Johnsson, Anna-Karin E, primary, Spence, Heather J, primary, Olson, Michael, primary, Machesky, Laura M., primary, Sansom, Owen J, primary, P. Morton, Jennifer, primary, Mitchell, Christina A., primary, S. Samuel, Michael, primary, Croucher, David R., primary, Welch, Heidi C. E., primary, Blyth, Karen, primary, Caldon, C. Elizabeth, primary, Herrmann, David, primary, I Anderson, Kurt, primary, Timpson, Paul, primary, and Nobis, Max, primary

Published
2023
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10. Single-Cell Omics Analyses Identify the TIM-3 Ligand Galectin-9 As Novel Immunotherapy Target for Chronic Lymphocytic Leukemia

Author

Llaó Cid, Laura, primary, Wong, John KL, additional, Wierz, Marina, additional, Paul, Yashna, additional, Roider, Tobias, additional, Fernandez Botana, Iria, additional, Gonder, Susanne, additional, Floerchinger, Alessia, additional, Colomer, Dolors, additional, Campo, Elías, additional, Dietrich, Sascha, additional, Lichter, Peter, additional, Zapatka, Marc, additional, Moussay, Etienne, additional, Paggetti, Jérôme, additional, and Seiffert, Martina, additional

Published
2022
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12. Optimizing metastatic-cascade-dependent Rac1 targeting in breast cancer: Guidance using optical window intravital FRET imaging

Author

Laura M. Machesky, Sharissa L. Latham, Karen Blyth, Owen J. Sansom, Paul Timpson, Cris S. Guaman, Victoria Lee, Nicola Ferrari, Anaiis Zaratzian, Sean C. Warren, Susan M. Mason, Pauline Mélénec, Lisa M Ooms, C. Elizabeth Caldon, Andrew M. Da Silva, Andrew T. McCulloch, Michael F. Olson, Xanthe L. Metcalf, Ghazal Sultani, Monica Phimmachanh, David Herrmann, Michael Tayao, Max Nobis, Alessia Floerchinger, Janett Stoehr, Jennifer P. Morton, Anna-Karin E. Johnsson, Christina Anne Mitchell, Heather J. Spence, Kendelle J. Murphy, David R. Croucher, Sonia Rolo, Heidi C.E. Welch, Young-Kyung Lee, Michael S. Samuel, Laura McDonald, Kurt I. Anderson, Floerchinger, Alessia, Murphy, Kendelle J, Latham, Sharissa L, Warren, Sean C, Samuel, Michael S, and Nobis, Max

Subjects
rac1 GTP-Binding Protein, FLIM, Lung Neoplasms, Cell Survival, QH301-705.5, drug response, RAC1, Breast Neoplasms, Biosensing Techniques, FRET biosensors, Tumor vasculature, small GTPases, General Biochemistry, Genetics and Molecular Biology, Metastasis, Imaging, Mice, Breast cancer, Cell Movement, Cell Line, Tumor, Fluorescence Resonance Energy Transfer, Medicine, Animals, Humans, metastasis, Biology (General), Metastatic cascade, Mice, Inbred BALB C, RAc1 activity, business.industry, imaging, Intravital Imaging, medicine.disease, Förster resonance energy transfer, Pyrimidines, Tumor progression, Cancer research, Aminoquinolines, Female, intravital imaging, single-cell intravital imaging, business, Shear Strength, Rac1, Signal Transduction
Abstract

Assessing drug response within live native tissue provides increased fidelity with regards to optimizing efficacy while minimizing off-target effects. Here, using longitudinal intravital imaging of a Rac1-Forster resonance energy transfer (FRET) biosensor mouse coupled with in vivo photoswitching to track intratumoral movement, we help guide treatment scheduling in a live breast cancer setting to impair metastatic progression. We uncover altered Rac1 activity at the center versus invasive border of tumors and demonstrate enhanced Rac1 activity of cells in close proximity to live tumor vasculature using optical window imaging. We further reveal that Rac1 inhibition can enhance tumor cell vulnerability to fluid-flow-induced shear stress and therefore improves overall anti-metastatic response to therapy during transit to secondary sites such as the lung. Collectively, this study demonstrates the utility of single-cell intravital imaging in vivo to demonstrate that Rac1 inhibition can reduce tumor progression and metastases in an autochthonous setting to improve overall survival.

Published
2021
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13. NK Cell Effector Functions and Bystander Tumor Cell Killing in Immunovirotherapy.

Author

Floerchinger A and Engeland CE

Subjects
Cell Death, Humans, Immunotherapy methods, Killer Cells, Natural, Measles virus genetics, Tumor Microenvironment, Neoplasms genetics, Oncolytic Virotherapy methods, Oncolytic Viruses genetics
Abstract

Oncolytic virotherapy is a compelling strategy to combine cancer gene therapy with immunotherapy. Lytic virus replication in malignant cells not only enables localized transgene expression based on engineered vectors but also triggers immunogenic tumor cell death and elicits inflammation in the tumor microenvironment. Modified oncolytic viruses encoding immunomodulators have been developed to enhance antitumor immune effects and therapeutic efficacy. As one example, bispecific molecules that engage immune cells to exert antitumor cytotoxicity can be encoded within the viral vector. This chapter describes an in vitro coculture experiment to study functionality and antitumor efficacy of engineered measles vaccine strain virus encoding natural killer cell engagers. In a flow cytometry-based analysis, target cell death of noninfected bystander cancer cells and effector functions of primary human natural killer cells are investigated. This methodology can facilitate assessment of advanced oncolytic viral vectors for cancer immunotherapy., (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2022
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