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Finalyse, a T4 bacteriophage, is a pre-harvest intervention that utilizes a combination of bacteriophages to reduce incoming Escherichia coli O157:H7 prevalence by destroying the bacteria on the hides of harvest-ready cattle entering commercial abattoirs. The objective of this study was to evaluate the efficacy of Finalyse, as a pre-harvest intervention, on the reduction in pathogens, specifically E. coli O157:H7, on the cattle hides and lairage environment to overall reduce incoming pathogen loads. Over 5 sampling events, a total of 300 composite hide samples were taken using 25 mL pre-hydrated Buffered Peptone Water (BPW) swabs, collected before and after the hide wash intervention, throughout the beginning, middle, and end of the production day (n = 10 swabs/sampling point/timepoint). A total of 171 boot swab samples were also simultaneously taken at the end of the production day by walking from the front to the back of the pen in a pre-determined 'Z' pattern to monitor the pen floor environment from 3 different locations in the lairage area. The prevalence of pathogens was analyzed using the BAX® System Real-Time PCR Assay. There were no significant reductions observed for Salmonella and/or any Shiga toxin-producing E. coli (STEC) on the hides after the bacteriophage application (p > 0.05). Escherichia coli O157:H7 and O111 hide prevalence was very low throughout the study; therefore, no further analysis was conducted. However, boot swab monitoring showed a significant reduction in E. coli O157:H7, O26, and O45 in the pen floor environment (p < 0.05). While using Finalyse as a pre-harvest intervention in the lairage areas of commercial beef processing facilities, this bacteriophage failed to reduce E. coli O157:H7 on the hides of beef cattle, as prevalence was low; however, some STECs were reduced in the lairage environment, where the bacteriophage was applied. Overall, an absolute conclusion was not formed on the effectiveness of Finalyse and its ability to reduce E. coli O157:H7 on the hides of beef cattle, as prevalence on the hides was low. [ABSTRACT FROM AUTHOR]

Recently, multiple reports from regulatory agencies have linked leafy green outbreaks to nearby or adjacent cattle operations. While they have made logical explanations for this phenomenon, the reports and data should be summarized to determine if the association was based on empirical data, epidemiological association, or speculation. Therefore, this scoping review aims to gather data on the mechanisms of transmission for pathogens from livestock to produce, identify if direct evidence linking the two entities exists, and identify any knowledge gaps in the scientific literature and public health reports. Eight databases were searched systematically and 27 eligible primary research products, which focus on produce safety concerning proximity to livestock, provided empirical or epidemiological association and described mechanisms of transmission, qualitatively or quantitatively were retained. Fifteen public health reports were also covered. Results from the scientific articles provided evidence that proximity to livestock might be a risk factor; however, most lack quantitative data on the relative contribution of different pathways for contamination. Public health reports mainly indicate livestock presence as a possible source and encourage further research. Although the collected information regarding the proximity of cattle is a concern, data gaps indicate that more studies should be conducted to determine the relative contribution of different mechanisms of contamination and generate quantitative data to inform food safety risk analyses, regarding leafy greens produced nearby livestock areas. [ABSTRACT FROM AUTHOR]