1. Synthetic antimicrobial Nkl and Dic peptides are immunomodulatory but only Dic peptide can be therapeutic against nodavirus infection.
- Author
-
Cervera L, Arizcun M, Mercado L, Chaves-Pozo E, and Cuesta A
- Subjects
- Animals, Proteolipids pharmacology, Proteolipids immunology, Fish Proteins immunology, Fish Proteins genetics, Fish Proteins pharmacology, Fish Proteins chemistry, Antimicrobial Peptides pharmacology, Antimicrobial Peptides chemistry, Immunomodulating Agents pharmacology, Immunomodulating Agents chemistry, Nodaviridae physiology, Bass immunology, Fish Diseases immunology, Fish Diseases drug therapy, Fish Diseases virology, RNA Virus Infections immunology, RNA Virus Infections veterinary, RNA Virus Infections drug therapy
- Abstract
Aquaculture is a prosperous economic sector threatened by viral infections. Among the viruses threatening fish culture, Betanodavirus (NNV) is extremely important in the Mediterranean Sea affecting to highly traded species as European sea bass. In this context, application of antimicrobial peptides (AMPs) has arisen as a potential biotechnological tool. The aim of this work was to evaluate the therapeutic application of two European sea bass-derived AMPs, NK-lysin (Nkl) and dicentracin (Dic), against NNV infections. Synthetic Dic peptide was able to significantly reduce NNV-induced mortalities while Nkl failed to do so. Although neither Dic nor Nkl peptides were able to alter the transcriptional levels of NNV and the number of infected cells, Nkl seemed to increase the viral load per cell. Interestingly, both Nkl and Dic peptides showed immunomodulatory roles. For instance, our data revealed an interplay among different AMPs, at both gene and protein levels. Otherwise, Nkl and Dic peptides provoked an anti-inflammatory balance upon NNV infection, as well as the recruitment of macrophages and B cells to the target site of the infection, the brain. In conclusion, Dic can be proposed as a therapeutic candidate to combat NNV., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF