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This study was conducted with the objective of evaluating the impact of replacing inorganic mineral sources (IM) with amino acid complexed minerals (AACM) in laying hens' diets on performance, egg quality, bone, and intestinal health. The effects of 4 different diets with varying levels of AACM substitution were evaluated on 400 Lohmann White hens aged 78-98 weeks. The control diet contained only IM sources at levels of 60, 60, 7, 40, 0.2, and 2 mg/kg of Zn, Mn, Cu, Fe, Se, and I, respectively. The other treatments were made by a total substitution of IM with AACM, as follows: AACM70-70% of IM levels; AACM50-50% of IM levels; and AACM40-40% of IM levels. Orthogonal polynomial contrasts and Dunnett's test were used to determine their impact (P < 0.05). The treatment AACM40 improved egg production, egg weight, egg mass, and feed conversion ratio (P < 0.05). Hens that received AACM40 also produced the thickest eggshells and better tibial bone density (P < 0.01). Histomorphometry analyses demonstrated significant effects of AACM treatments. The optimal supplementation levels of 24, 24, 2.8, 16, 0.08, and 0.8 mg/kg of Zn, Mn, Cu, Fe, Se, and I, respectively., (© 2024. The Author(s).)

This study investigated the optimal dietary intake of zinc amino acid complex (Zn-AAC) for white-layer pullets, focusing on their productive performance, biochemical profile, organ biometry, and body zinc retention. The study involved 360 Dekalb White pullets (average weight: 433 ± 4.42 g) aged from 6 to 16 weeks and distributed into 6 treatments with 6 replications each. The Zn-AAC inclusion levels ranged from 5 to 75 mg kg-1. Zinc intake was modeled using a nonlinear equation, Y = ∝ *1- e-βX, where α is the maximum response, β is the rate at which the response approaches the maximum (P < 0.05). The Zn-AAC supplementation had significant effects on average daily gain (ADG), average daily feed intake (ADFI), and feed convention ratio (FCR) (P < 0.01). Optimal intake levels of Zn-AAC were estimated at 0.234, 0.340, and 0.315 mg bird-1 day-1 (5.42, 7.87, and 7.30 mg kg-1) for ADG, ADFI, and FCR, respectively. The Zn-AAC supplementation affected the Zn body retention in pullets (P < 0.01), with an optimal ingestion at 1.86 mg bird -1 day-1, corresponding to a dietary supplementation of 43.10 mg kg-1. Additionally, supplementation affected alkaline phosphatase (ALP) activity (P < 0.01) without significant changes in aspartate aminotransferase, albumin, and globulin levels. The optimal Zn-AAC intake level for ALP activity was 1.45 mg bird -1 day-1, corresponding to dietary supplementation of 33.60 mg kg-1. Based on Zn body retention, we recommend up to 1.86 mg bird -1 day-1 of Zn-AAC, which is equivalent to 43.10 mg kg-1., Competing Interests: We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, and there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the content of this paper. The authors have declared that no competing interests exist., (Copyright: © 2024 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

A study was conducted to evaluate the effects of different sources and levels of supplemental amino acid-complexed minerals (AACM), with and without enzyme phytase (EZ). A total of 512 Dekalb White laying hens at 67 weeks of age were used in a 2 × 3 + 2 factorial arrangement of 8 treatments and 8 replications each. The main effects included EZ supplementation (600 FTU kg -1 ) and AACM inclusion level (100%, 70%, and 40% of inorganic mineral recommendations), plus two control treatments. The group of hens fed AACM-100 showed lower feed intake than the inorganic mineral (IM) group. The diet containing AACM-EZ-70 provided a higher ( p < 0.05) laying percentage and a lower ( p < 0.05) feed conversion ratio than both the IM and IM-EZ diets. The groups fed AACM-EZ-40, AACM-EZ-100, and AACM-70 produced heavier yolks ( p < 0.05). Hens fed IM laid eggs with the lowest yolk and albumen weights ( p < 0.05). Layers fed with AACM-100 and AACM-70 produced the most resistant eggshells to breakage ( p < 0.05). In diets containing phytase, the optimal AACM recommendations for better performance and egg quality in older laying hens are: 42, 49, 5.6, 28, 0.175, and 0.70 mg kg -1 for Zn, Mn, Cu, Fe, Se, and I, respectively.

In the intestinal lumen, excess of oxides and sulfates interfere with the absorption of minerals due to competition from the same absorption site. Amino acids-mineral complexed (AACM) is intended to minimize these problems, which might be absorbed by different absorption sites. Then, a study including Zinc (Zn), Manganese (Mn) and Copper (Cu) from different sources was carried out to evaluate the performance, blood parameters and reproductive organs development of Brown Laying Hens. A total of 800 Lohmann Brown Lite were fed, from one-day-old to 182-days-old, Zn, Mn and Cu from different sources. Measurements were made from 105 to 182-days-old. The laying hens were distributed according to a completely randomized design with 20 replicates and 20 birds per experimental unit. The treatments consisted of a diet supplemented with 70, 70 and 8 mg/kg of Zn, Mn and Cu; respectively, from inorganic sources (IM). The second treatment contained 40, 40 and 2.75 mg/kg of Zn, Mn and Cu, respectively from IM plus 30, 30 and 5.25 mg/kg of Zn, Mn and Cu; respectively, from AACM sources. Performance and reproductive organs development (oviduct and ovary weight), tibia weight, liver weight, egg output and body weight, and blood variables were evaluated. Data were compared by Student's t-test (P < 0.05). Laying hens fed AACM reached 35% of egg output two days earlier and presented heavier tibia bone than the IM group. Those hens also presented greater oviduct weight, greater hematocrit and greater serum concentration of total leukocytes, erythrocytes, eosinophils, monocytes and the hormones T4 and FSH, than the hens fed IM. The supplementation of AACM in laying hens' diets since one-day-old improves the productive performance from the beginning of egg output to peak production, which is justified by better development of bones and oviduct, hormone production and immune system support., Competing Interests: The authors have declared that no competing interests exist.

Two experiments were designed to evaluate the effect of mineral-amino acid complexes (AACM) as a partial replacement of inorganic mineral (IM) in layer-type chicks' diets. Both studies had the same dietary treatments, where in experiment 1 (Exp. 1) was conducted under thermoneutral conditions from 0 to 35 D and chicks in experiment 2 (Exp. 2) were exposed to cold stress conditions at nighttime during the first 15 D and to thermoneutral condition from 16 to 35 D. For each trial, 1,200 one-day-old Lohmann Brown chicks were used, with 20 cage replicates with 30 chicks per cage. Treatments consisted of the control diet (IM; with 70, 70, and 8 mg/kg of zinc [Zn], manganese [Mn], and copper [Cu], respectively) and the treatment diet (AACM, with 40, 40, and 2.75 mg/kg of Zn, Mn, and Cu, respectively, from IM sources, along with 30, 30, and 5.25 mg/kg of Zn, Mn, and Cu, respectively). Data were submitted to analysis of variance, and means were compared using the t-test (P < 0.05). In Exp. 1, there were no significant differences between treatments on chick performance. However, AACM-fed chicks had higher thymus (P = 0.03) and cecum weight (P < 0.01), superior micromineral deposition in the tibias (P < 0.01), and reduced phosphorus excretion (P = 0.03). In Exp. 2, chicks fed with AACM had higher body weight gain (P = 0.04), better average daily feed intake (P = 0.03), lower phosphorus excretion (P = 0.02), and higher liver and pancreas weight (P < 0.01) in the last week of the study. In conclusion, chicks fed with AACM under thermoneutral conditions had higher bone mineralization and reduced excretion of phosphorus, and in adverse conditions, AACM improves performance and liver and pancreas weight, also reducing phosphorus excretion., (Copyright © 2020. Published by Elsevier Inc.)

A study was conducted to evaluate growth performance, carcass and breast yields, and the occurrence and severity of white striping (WS) and wooden breast (WB) myopathies of broilers fed diets supplemented with increasing dietary levels of an organic source of selenium (Zn-L-SeMet). Broilers were fed 6 treatments with 12 replications of 26 birds in a 4-phase feeding program from 1 to 42 days. Corn-soy-based diets were supplemented with 0, 0.2, 0.4, 0.6, 0.8, and 1.0 ppm of Zn-L-SeMet. At 42 d, 6 birds were randomly selected from each pen (n = 72) and processed for carcass and breast yields. Breast fillets were scored for WS and WB at 42 days. Increasing Zn-L-SeMet led to quadratic responses (P < 0.05) for FCR from 1 to 7 d, BWG from 22 to 35 d, and for both responses from 8 to 21 d and 36 to 42 d, as well as in the overall period of 42 days. Carcass and breast yields presented a quadratic improvement (P < 0.01) with increasing Zn-L-SeMet supplementation and Se requirements were estimated at 0.85 and 0.86 ppm, respectively. In the overall period, estimates of Se requirements were 0.64 ppm for BWG and 0.67 ppm for FCR. White striping and WB scores presented quadratic increases (P < 0.01), and maximum scores were observed at 0.68 and 0.67 ppm, respectively. Broilers fed diets formulated without Se supplementation had a higher percentage of normal fillets compared to other Se supplementation levels (quadratic, P < 0.05). In conclusion, increasing Se supplementation to reach maximum growth performance led to higher degrees of severity of WS and WB. Selenium requirements determined in the present study were significantly higher than the present commercial recommendations.