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1. Telomere Attrition Is Associated With Sarcoidosis

Author

Gandhi, S.S., primary, Lee, C., additional, Yu, S., additional, Saber, T., additional, Woo, S., additional, Mcgovern, J., additional, Fiorini, V., additional, Gulati, M., additional, Herzog, E., additional, and Ryu, C., additional

Published
2024
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3. MATERNIDADE, PARENTALIDADE E CONJUGALIDADE: NOVAS PERSPECTIVAS EM PSICOLOGIA PERINATAL

Author

BENINCASA, M., primary, ROMAGNOLO, A. N., additional, HELENO, MARIA GERALDA VIANA, additional, ARRAIS, Alessandra da Rocha, additional, FONSECA, ANA, additional, Neumann, A. P., additional, BIAGI, ATALIA CRISTINE ÁVILA DE, additional, AMORIM, BIANCA, additional, PEDROTTI, B. G., additional, MOSMANN, C., additional, CENCI, C. M. B., additional, ANDRADE, C. J., additional, Merletti, Cristina Keiko Inafuku, additional, COSTA, C. B., additional, AZEVEDO, E. C., additional, ABUCHAIM, ERIKA DE SÁ VIEIRA, additional, MONTEIRO, FABIANA, additional, SCORSOLINI-COMIN, F., additional, SOUZA, F. C., additional, ALMEIDA, FRANCIELI DE SOUZA DE QUEIROZ, additional, GEHLEN, G., additional, FRIZZO, G. B., additional, AVOGLIA, Hilda Rosa Capelão, additional, SOUZA, H. P., additional, FIGUEIREDO, INÊS, additional, LEAL, ISABEL, additional, Silva, I. M., additional, JUSTO, JOÃO MANUEL ROSADO DE MIRANDA, additional, RAZERA, J., additional, QUAYLE, J., additional, ROCHA, LUCIANA, additional, RZYBOWSKI, LUCIANA SUÁREZ, additional, MALLMANN, M. Y., additional, PIETA, M. A. M., additional, CANAVARRO, MARIA CRISTINA, additional, HUEB, M.F.D., additional, RODRIGUES, Olga Maria Piazentin Rolim, additional, Coppola,, P., additional, AQUINO, R. R., additional, CAMPOS, S. O., additional, CHIODELLI, T., additional, FIORINI, V. R., additional, and PEREIRA, V. A., additional

Published
2020
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4. Serum Collagen Neoepitopes Are Associated With Multi-organ Disease in the Access and GRADS Sarcoidosis Cohorts

Author

Ryu, C., primary, Sand, J.M.B., additional, Juhl, P., additional, Jessen, H., additional, Leeming, D.J., additional, Hu, B., additional, Sun, Y., additional, Adams, T., additional, Pivarnik, T., additional, Yu, S., additional, Woo, S., additional, McGovern, J., additional, Fiorini, V., additional, Goobie, G.C., additional, Zhang, Y., additional, and Herzog, E.L., additional

Published
2023
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8. Clinical application of lung ultrasound score on COVID-19 setting: a regional experience in Southern Italy

Author

Lugara, M., Oliva, G., Pafundi, P. C., Tamburrini, S., Nevola, R., Gjeloshi, K., Ricozzi, C., Imbriani, S., Padula, A., Aprea, C., Meo, L., Cozzolino, D., Cuomo, G., Marrone, A., Fiorini, V., Coppola, M. G., Corvino, M., Perrella, A., Ponti, G., Nunnari, G., Ranieri, R., Ruosi, C., Sasso, F. C., Adinolfi, L. E., Rinaldi, L., Romano, C., Lugara, M., Oliva, G., Pafundi, P. C., Tamburrini, S., Nevola, R., Gjeloshi, K., Ricozzi, C., Imbriani, S., Padula, A., Aprea, C., Meo, L., Cozzolino, D., Cuomo, G., Marrone, A., Romano, C., Fiorini, V., Coppola, M. G., Corvino, M., Perrella, A., Ponti, G., Nunnari, G., Ranieri, R., Ruosi, C., Sasso, F. C., Adinolfi, L. E., and Rinaldi, L.

Subjects
Male, Lung ultrasound, COVID19, COVID-19, Middle Aged, Blood Gas Analysi, Hospitalization, Blood gas analysis, Prospective Studie, Italy, Intensive care, LUS score, Humans, Female, Prospective Studies, Blood Gas Analysis, Lung, Aged, Ultrasonography, Human
Abstract

OBJECTIVE: We aimed to assess the correlation between LUS Soldati proposed score and clinical presentation, course of disease and the possible need of ventilation support/intensive care. PATIENTS AND METHODS: All consecutive patients with laboratory confirmed SARS-CoV-2 infection and hospitalized in two COVID Centers were enrolled. All patients performed blood gas analysis and lung ultrasound (LUS) at admission. The LUS acquisition was based on standard sequence of 14 peculiar anatomic landmarks with a score between 0-3 based on impairment of LUS picture. Total score was computed with their sum with a total score ranging 0 to 42, according to Soldati LUS score. We evaluated the course of hospitalization until either discharge or death, the ventilatory support and the transition in intensive care if needed. RESULTS: One hundred and fifty-six patients were included in the final analysis. Most of patients presented moderate-to-severe respiratory failure (FiO2

Published
2021

9. Stewardship antimicrobica nel neonato e nel piccolo lattante: Perché e come praticarla

Author

Berardi, Alberto, Ficara, Monica, Pietrella, Elisa, Boncompagni, Alessandra, Toffoli, Carlotta, Bianchini, Anastasia, Della Casa, Elisa, Spada, Caterina, Spaggiari, Eugenio, Ciccia, Matilde, Gargano, Giancarlo, Rizzo, Vittoria, Azzalli, Milena, Biasucci, Giacomo, Gambini, Lucia, Bolognesi, Serenella, Ferrari, Fabrizio, Memo, L., Nicolini, G., Ciccia, M., Sandri, F., Capretti, M. G., Dondi, A., Pasini, L., Ragni, L., Albarelli, A., Fiorini, V., Giugno, C., Lanzoni, P., Di Grande, E., Polese, A., China, M. C., Rizzo, V., Stella, M., Zucchini, A., Malaguti, L., Azzalli, M., Garani, G., Nasi, S., Bacchini, P., Fragni, G., Baldassarri, P., Pulvirenti, R. M., Valletta, E., Bidetti, M. L., Incerti, S. Storchi, Di Carlo, C., Lanzoni, A., Serra, L., Silvestrini, D., Berardi, A., Ferrari, F., Lugli, L., Venturelli, C., Sarti, M., Volta, A., Dodi, I., Magnani, C., Guidi, B., Biasucci, G., Chiarabini, R., De Paulis, N., Padrini, D., Riboni, S., Pedna, M. F., Casadio, L., Marchetti, F., Muratori, C., Piccinini, G., Renzelli, C., Amarri, S., Baroni, L., Carretto, E., Gargano, G., Pedori, S., Riva, M., Zuelli, C., Ancona, G., Bolognesi, S., Vergine, G., Viola, L., Chiossi, C., Pagano, R., Zanacca, C., Palmieri, R., PICCININI, GIANCARLO, BASTELLI, ANNALISA, AMBRETTI, SIMONE, CORVAGLIA, LUIGI TOMMASO, LANARI, MARCELLO, LAMA, CARLA, VENTUROLI, VINCENZO, ALESSANDRINI, CATERINA, FACCHINETTI, FABRIZIO, GAMBINI, LUIGI, BERTELLI, MICHELE, SAMBRI, VITTORIO, FORNACIARI, SILVIA, PAPA, IRENE, RIVI, CLAUDIA, BONVICINI, CARLOTTA, Berardi, Alberto, Ficara, Monica, Pietrella, Elisa, Boncompagni, Alessandra, Toffoli, Carlotta, Bianchini, Anastasia, Della Casa, Elisa, Spada, Caterina, Spaggiari, Eugenio, Ciccia, Matilde, Gargano, Giancarlo, Rizzo, Vittoria, Azzalli, Milena, Biasucci, Giacomo, Gambini, Lucia, Bolognesi, Serenella, Piccinini, Giancarlo, Ferrari, Fabrizio, Memo, L., Nicolini, G., Ciccia, M., Bastelli, A., Sandri, F., Ambretti, S., Capretti, M. G., Corvaglia, L., Dondi, A., Lanari, M., Pasini, L., Ragni, L., Albarelli, A., Fiorini, V., Giugno, C., Lanzoni, P., Di Grande, E., Polese, A., China, M. C., Rizzo, V., Stella, M., Zucchini, A., Malaguti, L., Azzalli, M., Garani, G., Lama, C., Nasi, S., Bacchini, P., Fragni, G., Baldassarri, P., Pulvirenti, R. M., Valletta, E., Venturoli, V., Alessandrini, C., Bidetti, M. L., Incerti, S. Storchi, Di Carlo, C., Lanzoni, A., Serra, L., Silvestrini, D., Berardi, A., Facchinetti, F., Ferrari, F., Lugli, L., Venturelli, C., Sarti, M., Volta, A., Dodi, I., Gambini, L., Magnani, C., Guidi, B., Bertelli, M., Biasucci, G., Chiarabini, R., De Paulis, N., Padrini, D., Riboni, S., Pedna, M. F., Sambri, V., Casadio, L., Marchetti, F., Muratori, C., Piccinini, G., Renzelli, C., Amarri, S., Baroni, L., Carretto, E., Fornaciari, S., Gargano, G., Pedori, S., Riva, M., Zuelli, C., Ancona, G., Bolognesi, S., Papa, I., Vergine, G., Viola, L., Chiossi, C., Pagano, R., Rivi, C., Zanacca, C., Bonvicini, C., and Palmieri, R.

Subjects
Sepsi, Pediatrics, Perinatology and Child Health, Antibiotic, Antimicrobial stewardship, Prophylaxi, Infection, Newborn
Abstract

Antimicrobial resistances are increasing worldwide, due to the misuse of antibiotic therapies in humans as well as in animals. It is expected that within a few decades multi-resistant pathogens will become the leading cause of death worldwide. The misuse of antibiotics in the neonatal period depends on the lack of predictive diagnostic tests and the peculiarity of symptoms of sepsis, which frequently are vague and unspecific at the time of presentation. Policies to prevent the emergence of antimicrobial resistances rely on a judicious use of antibiotics, on protocols and additional measures shared by health care providers. This document summarizes recent indications issued in the Emilia-Romagna subsequent to a review of the literature.

Published
2017

10. Highly conjugated blue dimers of platinum(II)

Author

Esposito, R., CALVANESE, LUISA, CUCCIOLITO, MARIA ELENA, D'AURIA, GABRIELLA, FALCIGNO, LUCIA, Fiorini, V., Pezzella, P., Roviello, G., RUFFO, FRANCESCO, Stagni, S., TALARICO, GIOVANNI, Società Chimica Italiana - Co.G.I.C.O., Esposito, R., Calvanese, Luisa, Cucciolito, MARIA ELENA, D'Auria, Gabriella, Falcigno, Lucia, Fiorini, V., Pezzella, P., Roviello, G., Ruffo, Francesco, Stagni, S., and Talarico, Giovanni

Published
2016

11. Luminescent protein staining with Re(i) tetrazolato complexes

Author

Fiorini, V., Bergamini, L., Monti, N., Zacchini, S., Plush, S., Massi, Massimiliano, Hochkoeppler, A., Stefan, A., Stagni, S., Fiorini, V., Bergamini, L., Monti, N., Zacchini, S., Plush, S., Massi, Massimiliano, Hochkoeppler, A., Stefan, A., and Stagni, S.

Abstract

Within the general framework of our past and current studies dealing with the investigation of the photophysical properties and the biological behavior of the family of tetrazolato and tetrazole Re(i) complexes, we have endeavored to investigate their potential in the luminescent staining of proteins purified by acrylamide gel electrophoresis. With the aim to provide the first examples of luminescent Re(i) complexes to be exploited for this specific purpose, we have designed and prepared four new Re(i)-based species with the general formula fac-[Re(CO)3(N^N)(Tph)]2-/0, where Tph is the 5-(phenyl)tetrazolato anion and N^N is in turn represented by bathophenanthroline disulfonate (BPS), bathocuproine disulfonate (BCS) or by the SO3-free bathocuproine (BC). In this latter case, the neutral complex fac-[Re(CO)3(BC)(Tph)] served as a model species for the characterization of the former disulfonate complexes. Its cationic analogue fac-[Re(CO)3(BC)(Tph-Me)]+was also prepared by a straightforward methylation reaction. All complexes displayed bright phosphorescence in organic media and, relative to their water solubility, the dianionic species fac-[Re(CO)3(BPS)(Tph)]2-and fac-[Re(CO)3(BCS)(Tph)]2-were also highly emissive in aqueous solution. The sulfonate groups played a key role in promoting and significantly enhancing the luminescent staining performances of both the Re(i) complexes fac-[Re(CO)3(BPS)(Tph)]2-and fac-[Re(CO)3(BCS)(Tph)]2-for proteins. Highlighting a response superior to that of Coomassie Blue and comparable to the one obtained by the well-known silver staining method, these dianionic Re(i)-complexes could efficiently detect up to 50 ng of pure Bovine Serum Albumin (BSA), as well as all proteins found in a Standard Protein Marker mix and from a total protein extract. A lower but still good response for luminescent protein staining was surprisingly obtained by employing the-SO3-free neutral and cationic complexes fac-[Re(CO)3(BC)(Tph)] and fac-[Re(CO)3(BC)(Tp

Published
2018

12. The burden of early-onset sepsis in Emilia-Romagna (Italy): a 4-year, population-based study

Author

Berardi, Alberto, Baroni, Lorenza, Bacchi Reggiani, Maria Letizia, Ambretti, Simone, Biasucci, Giacomo, Bolognesi, Serenella, Capretti, Maria Grazia, Carretto, Edoardo, Ciccia, Matilde, Fiorini, Valentina, Fortini, Cinzia, Gargano, Giancarlo, Pedna, Maria Federica, Rizzo, Vittoria, Creti, Roberta, Ferrari, Fabrizio, Memo, L., Nicolini, G., Campanile, A., Tridapalli, E., Ciccia, M., Bastelli, A., Sandri, F., Ambretti, S., Capretti, M. G., Gentili, A., Ragni, L., Albarelli, A., Piscina, A., Borghi, A., Simoni, A., Fiorini, V., Grande, E. D., Polese, A., Biasini, A., China, M. C., Rizzo, V., Zucchini, A., Malaguti, L., Contiero, R., Fortini, C., Garani, G., Rossi, M. R., Nasi, S., Bacchini, P., Baldassarri, P., Pulvirenti, R. M., Vaienti, F., Venturoli, V., Bidetti, M. L., Colla, R., Toniato, M., Carlo, C. D., Lanari, M., Serra, L., Silvestrini, D., Facchinetti, F., Ferrari, F., Lugli, L., Venturelli, C., Sarti, M., Volta, A., Dodi, I., Gambini, L., Guidi, B., Bertelli, M., Biasucci, G., Chiarabini, R., Padrini, D., Piepoli, M., Riboni, S., Rubbi, P., Pedna, M. F., Sambri, V., Perrone, A., Preti, P., Marchetti, F., Piccinini, G. C., Amarri, S., Carretto, E., Gargano, G., Pedori, S., Riva, M., Rossi, C., Zuelli, C., Bolognesi, S., Papa, I., Vergine, G., Viola, L., Chiossi, C., Pagano, R., Zanacca, C., Palmieri, R., Berardi, Alberto, Baroni, Lorenza, Bacchi Reggiani, Maria Letizia, Ambretti, Simone, Biasucci, Giacomo, Bolognesi, Serenella, Capretti, Maria Grazia, Carretto, Edoardo, Ciccia, Matilde, Fiorini, Valentina, Fortini, Cinzia, Gargano, Giancarlo, Pedna, Maria Federica, Rizzo, Vittoria, Creti, Roberta, Ferrari, Fabrizio, and GBS Prevention Working Group Emilia-Romagna [,Lanari, Marcello,]

Subjects
Pediatrics, Antibiotics, group B streptococcu, Logistic regression, medicine.disease_cause, Severity of Illness Index, Group B, 0302 clinical medicine, Risk Factors, Medicine, Birth Weight, 030212 general & internal medicine, Escherichia coli Infections, Streptococcus, Gestational age, meningitis, Obstetrics and Gynecology, Drug Resistance, Microbial, intrapartum chemoprophylaxi, Perinatology and Child Health, meningiti, Anti-Bacterial Agents, Italy, Regression Analysis, Neonatal Sepsis, Meningitis, Early-onset sepsis, group B streptococcus, intrapartum chemoprophylaxis, newborn infant, Pediatrics, Perinatology and Child Health, Infant, Premature, Cohort study, medicine.medical_specialty, medicine.drug_class, Statistics, Nonparametric, Streptococcus agalactiae, Sepsis, 03 medical and health sciences, 030225 pediatrics, Streptococcal Infections, Escherichia coli, Humans, Retrospective Studies, business.industry, Infant, Newborn, Infant, medicine.disease, Early-onset sepsi, Ampicillin, Gentamicins, business
Abstract

Objective: To provide the first Italian data on pathogens causing early-onset sepsis (EOS) and their antimicrobial susceptibility, after the successfully prevention of Group B streptococcus (GBS) EOS. Methods: Retrospective area-based cohort study from Emilia-Romagna (Italy). Cases of EOS registered (from 2009 to 2012) in all gestational age neonates were reviewed. Results: Live births (LB) numbered 146 682. Ninety neonates had EOS and 12 died (incidence rates of 0.61 and 0.08/1000 LB, respectively). EOS and mortality were the highest among neonates with a birth weight n = 27, 0.18/1000 LB) and Escherichia coli (n = 19, 0.13/1000 LB). Most infants affected by E. coli EOS were born preterm (n = 13), had complications (n = 4) or died (n = 7). Among 90 isolates tested, only 3 were resistant to both first line empirical antibiotics. Multivariate logistic regression analysis showed that low gestational age, caesarean section and low platelet count at presentation were significantly associated with death or brain lesions (area under ROC curve = 0.939, H-L = 0.944, sensitivity 76.0%, specificity 90.7%). Conclusions: GBS slightly exceeds E. coli as a cause of EOS. However, E. coli is the prominent cause of death, complications and in most cases affects preterm neonates. Empirical antimicrobial therapy of EOS seems appropriate.

Published
2015

13. Methylation of Ir(III)-tetrazolato complexes: An effective route to modulate the emission outputs and to switch to antimicrobial properties

Author

Fiorini, V., Zanoni, I., Zacchini, S., Costa, A., Hochkoeppler, A., Zanotti, V., Ranieri, Anna, Massi, Massimiliano, Stefan, A., Stagni, S., Fiorini, V., Zanoni, I., Zacchini, S., Costa, A., Hochkoeppler, A., Zanotti, V., Ranieri, Anna, Massi, Massimiliano, Stefan, A., and Stagni, S.

Abstract

Two neutral cyclometalated Ir(iii)-tetrazolato complexes that differ by variations of the substituents on either the phenylpyridine or the tetrazolate ligand have been converted into the corresponding methylated and cationic analogues. NMR ( 1 H and 13 C) characterization of the Ir(iii) complexes provided the results in agreement with the chemo- and regioselective character of methylation at the N-3 position of the Ir(iii)-coordinated tetrazolato ring. This evidence was further corroborated by the analysis of the molecular structures of the cationic complexes obtained by X-ray diffraction. In view of the photophysical properties, the addition of a methyl moiety to neutral Ir(iii) tetrazolates, which behave as sky-blue or orange phosphors, caused a systematic red shift of their phosphorescence output. The transformation of neutral Ir(iii) tetrazolates into cationic Ir(iii)-tetrazole complexes was screened for any eventual antimicrobial activity in vitro against Gram negative (E. coli) and Gram positive (D. radiodurans) microorganisms. While both kinds of complexes were not active against E. coli, the conversion of the neutral Ir(iii) tetrazolates into the corresponding methylated and cationic Ir(iii)tetrazole derivatives determined the turn-on of a good to excellent antimicrobial activity toward Gram positive Deinococcus radiodurans, a non-pathogenic bacterium that is listed as one of the toughest microorganisms in light of its outstanding resistance to radiation and oxidative stress.

Published
2017

14. Discrete-event model based simulation of a tobacco packing line

Author

PAOLI, ANDREA, BONIVENTO, CLAUDIO, MELANDRI L., FIORINI V., FERRARINI L., PAOLI A., BONIVENTO C., MELANDRI L., and FIORINI V.

Subjects
SIMULATION, DISCRETE-EVENT SYSTEMS, PACKAGING MACHINERY, DISTRIBUTED ARCHITECTURE, AUTOMATIC CONTROL
Abstract

In this work a logic model based on discrete event systems to simulate a production line is presented. After a short theoretical introduction, the plant under exam is described, the designed model (implemented in Matlab and Simulink) is deeply reviewed and its use, as well as the obtained results, is shown. This work has been realized as a cooperation between the company G.D, world leader in tobacco packaging, and the Center for Research on Complex Automated Systems (CASY) “Giuseppe Evangelisti” of the University of Bologna. [8 double column pages]

Published
2006

15. Negatively charged Ir(III) cyclometalated complexes containing a chelating bis-tetrazolato ligand: Synthesis, photophysics and the study of reactivity with electrophiles

Author

Fiorini, V., Zacchini, S., Raiteri, Paolo, Mazzoni, R., Zanotti, V., Massi, Massimiliano, Stagni, S., Fiorini, V., Zacchini, S., Raiteri, Paolo, Mazzoni, R., Zanotti, V., Massi, Massimiliano, and Stagni, S.

Abstract

The bis-tetrazolate dianion [1,2 BTB]2−, which is the deprotonated form of 1,2 bis-(1H-tetrazol-5-yl)benzene [1,2-H2BTB], is for the first time exploited as an ancillary N^N ligand for negatively charged [Ir(C^N)2(N^N)]−-type complexes, where C^N is represented by cyclometalated 2-phenylpyridine (ppy) or 2-(2,4-difluorophenyl)pyridine (F2ppy). The new Ir(III) complexes [Ir(ppy)2(1,2 BTB)]− and [Ir(F2ppy)2(1,2 BTB)]− have been fully characterised and the analysis of the X-ray structure of [Ir(ppy)2(1,2 BTB)]− confirmed the coordination of the [1,2 BTB]2− dianion in a bis chelated fashion through the N-atoms adjacent to each of the tetrazolic carbons. Both of the new anionic Ir(III) complexes displayed phosphorescence in the visible region, with intense sky-blue (λmax = 460–490 nm) or aqua (λmax = 490–520 nm) emissions originating from [Ir(F2ppy)2(1,2 BTB)]− and [Ir(ppy)2(1,2 BTB)]−, respectively. In comparison with our very recent examples of anionic Ir(III)tetrazolate cyclometalates, the new Ir(III) tris chelate complexes [Ir(F2ppy)2(1,2 BTB)]− and [Ir(ppy)2(1,2 BTB)]−, display an improved robustness, allowing the study of their reactivity toward the addition of electrophiles such as H+ and CH3+. In all cases, the electrophilic attacks occurred at the coordinated tetrazolate rings, involving the reversible – by a protonation deprotonation mechanism – or permanent – upon addition of a methyl moiety – switching of their global net charge from negative to positive and, in particular, the concomitant variation of their photoluminescence output. The combination of the anionic complexes [Ir(F2ppy)2(1,2 BTB)]− or [Ir(ppy)2(1,2 BTB)]− with a deep red emitting (λmax = 686 nm) cationic Ir(III) tetrazole complex such as [IrTPYZ-Me]+, where TPYZ-Me is 2-(2-methyl-2H-tetrazol-5-yl)pyrazine, gave rise to two fully Ir(III)-based soft salts capable of displaying additive and O2-sensitive emission colours, with an almost pure white light obtained by the appropriate choice of the ion

Published
2016

16. Fully Ir(iii) tetrazolate soft salts: the road to white-emitting ion pairs

Author

Fiorini, V., D'Ignazio, A., Magee, K., Ogden, Mark, Massi, M., Stagni, S., Fiorini, V., D'Ignazio, A., Magee, K., Ogden, Mark, Massi, M., and Stagni, S.

Abstract

The first examples of anionic Ir(iii) bis-tetrazolate complexes and their combination with a cationic Ir(iii)tetrazole derivative forming "fully tetrazolate" Ir(iii) based soft salts as O2-sensitive white emitters are described herein.

Published
2016

18. Un fulmine a ciel sereno

Author

Rossi, C, Cipolli, S, Fiorini, V, Torcetta, F, Cattani, S, Vezzani, E, Zagni, P, Baccilieri, F, Palmieri, M, and Ferrari, Fabrizio

Subjects
Sepsi batterica
Published
2011

19. Targeting divalent metal cations with Re(I) tetrazolato complexes

Author

Fiorini, V., Ranieri, A., Muzzioli, S., Magee, Karen, Zacchini, S., Akabar, Nurshadrina, Stefan, A., Ogden, Mark, Massi, Massimiliano, Stagni, S., Fiorini, V., Ranieri, A., Muzzioli, S., Magee, Karen, Zacchini, S., Akabar, Nurshadrina, Stefan, A., Ogden, Mark, Massi, Massimiliano, and Stagni, S.

Abstract

© 2015 The Royal Society of Chemistry. In order to exploit their potential as versatile luminescent sensors, four new Re(i)-tetrazolato complexes with the general formula fac-[Re(CO)3(diim)(L)], where diim is 2,2'-bipyridine (bipy) or 1,10-phenanthroline (phen) and L- is either the anion 5-(2'-pyridyl)tetrazolato (2-PTZ-) or 5-(2'-quinolyl)tetrazolato (2-QTZ-), were prepared and fully characterized. In all cases, the regioselective coordination of the Re(i) center through the N2 atom of the tetrazolato ring was observed. This particular feature ensures the availability of the diiminic (N^N) site that was systematically incorporated into the structure of the 2-PTZ- and 2-QTZ- ligands for further coordination with metal cations. Such a diimine-type coordination mode was preliminarily tested by using the mononuclear Re(i) complexes as N^N ligands for the preparation of two [(N^N)Cu(POP)] cationic species, where POP is the chelating diphosphine bis[2-(diphenylphosphino)phenyl]ether. The X-ray structures of the resulting Re(i)-Cu(i) dyads revealed that the Re(i) mononuclear complexes effectively behaved as chelating N^N ligands with respect to the [Cu(POP)]+ fragment, the coordination of which also resulted in significant modification of the Re(i)-centered luminescence. With these data in hand, the luminescent sensing abilities of the four new Re(i) tetrazolato complexes were screened with respect to divalent metal ions of toxicological and biological importance such as Zn(ii), Cd(ii) and Cu(ii). The interaction of the Re(i) complexes with Zn(ii) and Cd(ii) was witnessed by the evident blue shift (??max = 22-36 nm) of the emission maxima, which was also accompanied by a significant elongation of the emission lifetimes. On the contrary, the addition of the cupric ion caused substantial quenching of the radiative processes originating from the Re(i) luminophores.

Published
2015

20. Methylated Re(i) tetrazolato complexes: Photophysical properties and Light Emitting Devices

Author

Werrett, M., Huff, G., Muzzioli, S., Fiorini, V., Zacchini, S., Skelton, B., Maggiore, A., Malicka, J., Cocchi, M., Gordon, K., Stagni, S., Massi, Massimiliano, Werrett, M., Huff, G., Muzzioli, S., Fiorini, V., Zacchini, S., Skelton, B., Maggiore, A., Malicka, J., Cocchi, M., Gordon, K., Stagni, S., and Massi, Massimiliano

Abstract

The irreversible reaction of methyl triflate with neutral Re(I) tetrazolato complexes of the type fac-[Re(diim)(CO)3(L)], where diim is either 1,10-phenanthroline or 2,2′-bipyridine and L is a para substituted 5-aryltetrazolate, yielded the corresponding cationic methylated complexes. While methylation occurred regioselectively at the N4 position of the tetrazole ring, the cationic complexes were found to exist in solution as equilibrating mixtures of linkage isomers, where the Re(I) centre was bound to either the N1 or N2 atom of the tetrazole ring. The existence of these isomers was highlighted both by NMR and X-ray crystallography studies. On the other hand, the two isomers appeared indistinguishable by IR, UV-Vis and luminescence spectroscopy. The prepared cationic complexes are all brightly phosphorescent in fluid and rigid solutions, with emission originating from triplet metal-to-ligand charge transfer excited states. Compared to their neutral precursors, which emit from admixtures of triplet metal-to-ligand and ligand-to-ligand charge transfer states, the methylated complexes exhibit blue-shifted emission characterised by elongated excited state lifetimes and increased quantum yields. The nature of the excited states for both the neutral and the methylated complexes was probed by resonance Raman spectroscopy and with the aid of time-dependent density functional theory calculations. Lastly, both the neutral and the methylated species were used as emitting phosphors in the fabrication of Organic Light Emitting Diodes and Light Emitting Electrochemical Cells.

Published
2015

22. Diagnosi prenatale di sclerosi tuberosa

Author

Rossi, C., Frassoldati, Rossella, Fiorini, V., Guidotti, I., Cipolli, S., Guerra, A., and Iughetti, Lorenzo

Subjects
sclerosi tuberosa
Published
2010

24. Volvolo prenatale

Author

Fiorini, V., Rossi, K., Ceccarelli, P. L., Bianchini, M. A., Rivi, C., Bastoni, S., Bertoncelli, N., Di Pancrazio, L., Gallo, C., Cacciari, Alfredo, and Ferrari, Fabrizio

Subjects
volvolo prenatale
Published
2008

25. Due fratelli uniti da un unico destino?

Author

Mariotti, I, Garetti, E, Roversi, Mf, Della Casa, E, Fiorini, V, Pagano, R, Baroni, L, Dotti, D, Ori, L, Predieri, Barbara, Iughetti, Lorenzo, and Ferrari, Fabrizio

Subjects
due fratelli, unico destino
Published
2007

26. Un caso di encefalopatia ipossico-ischemica

Author

Lasagni, A, Lugli, L, Gallo, C, Berardi, A, Guerra, M, Fiorini, V, Ori, L, Saccani, I, Grimaldi, R, Bertoncelli, N, Mazzoli, M, and Ferrari, Fabrizio

Subjects
encefalopatia ipossico ischemica
Published
2007

30. Se il cuore decelera

Author

Paltrinieri, Al, Gargano, G, Gallo, C, Rivi, C, Fiorini, V, Sabbioni, F, Sala, B, Bonvicini, C, Guidotti, I, Predieri, Barbara, Iughetti, Lorenzo, and Ferrari, Fabrizio

Subjects
decelazione cardiaca
Published
2007

36. Uno strano caso di anemia neonatale

Author

Rivi, C, Gallo, C, Cellini, M, Cattani, Mordini, B, Cano, C, Leonardi, F, Fiorini, V, Ferrari, Fabrizio, and Paolucci, Paolo

Subjects
anemia neonatale
Published
2006

38. Perforazione gastrica in epoca neonatale

Author

Fiorini, V., Bianchini, Ma, Torcetta, F., Ferrari, Ma, Sassi, L., Di Pancrazio, L., Biondini, D., Ceccarelli, Pl, Ferrari, Fabrizio, Gallo, E., and Cacciari, Alfredo

Subjects
perforazione gastrica neonatale
Published
2006

40. Introducing a New Family of Biotinylated Ir(III)-Pyridyltriazole Lumophores: Synthesis, Photophysics, and Preliminary Study of Avidin-Binding Properties

Author

Baschieri, A., Muzzioli, S., Fiorini, V., Matteucci, E., Massi, Massimiliano, Sambri, L., Stagni, S., Baschieri, A., Muzzioli, S., Fiorini, V., Matteucci, E., Massi, Massimiliano, Sambri, L., and Stagni, S.

Abstract

Six new biotinylated reagents derived from monocationic pyridyl-1,2,3-triazole-based Ir(III) complexes of the general formula [(C∧N)2Ir(N∧N-spacer-X-CO-biotin)]+, where HC∧N is 2-phenylpyridine, N∧N is the neutral chelating (2-pyridyl)-1,2,3-triazole ligand, the term “spacer” refers to alkyl (−C11H22) or aromatic (p-phenyl- or 4,4′-biphenyl-) chains, and X is NH or O, have been prepared and characterized. Upon photoexcitation, all six complexes in fluid CH2Cl2 and aqueous solutions at room temperature displayed intense—with quantum yields as high as 0.60 (complex 4b)—and quite long-lived blue-green luminescence, corresponding in each case to a vibronically structured emission profile peaking at ca. 480 and ca. 508 nm. These features, in combination with the reduced rigidochromic shift that was observed from the same samples frozen at 77 K, suggested the 3LC-type excited states as the prevalent contributors to the emission. The interactions of these new biotinylated complexes with avidin have been studied by 4′-hydroxyazobenzene-2-carboxylic acid (HABA) assays and emission titrations. In general, the amplification of the emission intensity of the Ir(III) complexes occurring upon their interaction with avidin was observed. It is also worth mentioning how a similar or better response was displayed by [(C∧N)2Ir(N∧N-spacer-O-CO-biotin)]+-type complexes, in which biotin is appended to the Ir(III) lumophore through an ester moiety.

Published
2014

43. Antimicrobial stewardship in newborns and young infants: Why and how to do it,Stewardship antimicrobica nel neonato e nel piccolo lattante: Perché e come praticarla

Author

Berardi, A., Ficara, M., Pietrella, E., Boncompagni, A., Toffoli, C., Bianchini, A., Della Casa, E., Spada, C., Spaggiari, E., Ciccia, M., Gargano, G., Rizzo, V., Azzalli, M., Biasucci, G., Gambini, L., Bolognesi, S., Piccinini, G., Ferrari, F., Memo, L., Nicolini, G., Bastelli, A., Sandri, F., Ambretti, S., Capretti, M. G., Corvaglia, L., Dondi, A., Lanari, M., Pasini, L., Ragni, L., Albarelli, A., Fiorini, V., Giugno, C., Lanzoni, P., Di Grande, E., Polese, A., China, M. C., Stella, M., Zucchini, A., Malaguti, L., Garani, G., Lama, C., Nasi, S., Bacchini, P., Fragni, G., Baldassarri, P., Pulvirenti, R. M., Valletta, E., Venturoli, V., Alessandrini, C., Bidetti, M. L., Incerti, S. S., Di Carlo, C., Lanzoni, A., Serra, L., Silvestrini, D., Facchinetti, F., licia lugli, Venturelli, C., Sarti, M., Volta, A., Dodi, I., Magnani, C., Guidi, B., Bertelli, M., Chiarabini, R., Paulis, N., Padrini, D., Riboni, S., Pedna, M. F., Sambri, V., Casadio, L., Marchetti, F., Muratori, C., Renzelli, C., Amarri, S., Baroni, L., Carretto, E., Fornaciari, S., Pedori, S., Riva, M., Zuelli, C., Ancona, G., Papa, I., Vergine, G., Viola, L., Chiossi, C., Pagano, R., Rivi, C., Zanacca, C., Bonvicini, C., and Palmieri, R.

44. Antimicrobial stewardship in newborns and young infants: Why and how to do it,Stewardship antimicrobica nel neonato e nel piccolo lattante: Perché e come praticarla

Author

Berardi, A., Ficara, M., Pietrella, E., Boncompagni, A., Toffoli, C., Bianchini, A., Della Casa, E., Spada, C., Spaggiari, E., Ciccia, M., Gargano, G., Rizzo, V., Azzalli, M., Biasucci, G., Gambini, L., Bolognesi, S., Piccinini, G., Ferrari, F., Memo, L., Nicolini, G., Bastelli, A., Sandri, F., Ambretti, S., Capretti, M. G., Corvaglia, L., Dondi, A., Lanari, M., Pasini, L., Ragni, L., Albarelli, A., Fiorini, V., Giugno, C., Lanzoni, P., Di Grande, E., Polese, A., China, M. C., Stella, M., Zucchini, A., Malaguti, L., Garani, G., Lama, C., Nasi, S., Bacchini, P., Fragni, G., Baldassarri, P., Pulvirenti, R. M., Valletta, E., Venturoli, V., Alessandrini, C., Bidetti, M. L., Incerti, S. S., Di Carlo, C., Lanzoni, A., Serra, L., Silvestrini, D., Facchinetti, F., Lugli, L., Venturelli, C., Sarti, M., Volta, A., Dodi, I., Magnani, C., Guidi, B., Bertelli, M., Chiarabini, R., Paulis, N., Padrini, D., Riboni, S., Pedna, M. F., Sambri, V., Casadio, L., Marchetti, F., Muratori, C., Renzelli, C., Amarri, S., Baroni, L., Edoardo Carretto, Fornaciari, S., Pedori, S., Riva, M., Zuelli, C., Ancona, G., Papa, I., Vergine, G., Viola, L., Chiossi, C., Pagano, R., Rivi, C., Zanacca, C., Bonvicini, C., and Palmieri, R.

46. Colourless luminescent solar concentrators based on Iridium(III)-Phosphors

Author

Loris Giorgini, Greta Santi, Nicola Monti, Massimiliano Massi, Valentina Fiorini, Stefano Stagni, Giulia Vigarani, Michele Pavone, Francesca Fasulo, Ana B. Muñoz-García, Andrea Pucci, Fiorini, V., Monti, N., Vigarani, G., Santi, G., Fasulo, F., Massi, M., Giorgini, L., Munoz-Garcia, A. B., Pavone, M., Pucci, A., Stagni, S., Fiorini V., Monti N., Vigarani G., Santi G., Fasulo F., Massi M., Giorgini L., Munoz-Garcia A.B., Pavone M., Pucci A., and Stagni S.

Subjects
Materials science, Colourless LSCs, Ir(III) cyclometalated complexes, Luminescent solar concentrators, Phosphorescent metal complexes, Stokes-shifts, Tetrazole ligands, Transparent polymers, Colourless LSC, General Chemical Engineering, chemistry.chemical_element, Phosphor, 02 engineering and technology, 010402 general chemistry, Methacrylate, 01 natural sciences, chemistry.chemical_compound, Tetrazole ligand, Stokes-shift, Iridium, Methyl methacrylate, Acrylate, Luminescent solar concentrator, Process Chemistry and Technology, 021001 nanoscience & nanotechnology, Phosphorescent metal complexe, 0104 chemical sciences, chemistry, Ir(III) cyclometalated complexe, 2-Phenylpyridine, 0210 nano-technology, Luminescence, Phosphorescence, Nuclear chemistry
Abstract

The first real examples of luminescent solar concentrators (LSCs) based on Ir(III) cyclometalates, are described herein. Two new Ir(III) tetrazole complexes, namely [Ir(ppy)2(iQTZ-PPG)]+ and [Ir(npy)2(iQTZ-PPG)]+, where (C^N) = ppy, 2 phenylpyridine or npy = 2-(naphthalen-2-yl)pyridine and iQTZ-PPG = 1-(2-(prop-2-yn-1-yl)-2H-tetrazol-5-yl)isoquinoline, were synthesized, fully characterized and tested as phosphors for colourless LSCs. Notably, increasing quantities (0.2–1.8 wt %) of the new Ir(III) based phosphors were dispersed in different acrylate polymers like poly(methyl methacrylate), PMMA, poly(benzyl methacrylate), PBzMA and poly(cyclohexyl methacrylate), PCHMA, leading to visible transparent polymeric films exhibiting excellent photostability and bright yellow to orange phosphorescent emissions. The performances as solar collectors of all the Ir(III)-doped polymers were investigated, providing results comparable, or superior, to those obtained from colourless LSC based on organic fluorophores. In fact, the best optical efficiency (ηopt up to 7%, combined to transmittance close to 80% at 390 nm) was displayed by the polymer film obtained from physical dispersion of [Ir(ppy)2(iQTZ-PPG)]+ (1.4 wt %) in PCHMA.

Published
2021

47. Use of cotton textiles coated by ir(III) tetrazole complexes within ceramic silica nanophases for photo-induced self-marker and antibacterial application

Author

Matteo Crosera, Sotiris Psilodimitrakopoulos, Simona Ortelli, Emmanuel Stratakis, Valentina Fiorini, Stefano Stagni, Francesca Larese Filon, Ilaria Zanoni, Magda Blosi, Anna Luisa Costa, Alessandra Stefan, Zanoni I., Blosi M., Fiorini V., Crosera M., Ortelli S., Stagni S., Stefan A., Psilodimitrakopoulos S., Stratakis E., Filon F.L., Costa A.L., Zanoni, I., Blosi, M., Fiorini, V., Crosera, M., Ortelli, S., Stagni, S., Stefan, A., Psilodimitrakopoulos, S., Stratakis, E., Filon, F. L., and Costa, A. L.

Subjects
Materials science, General Chemical Engineering, Silica host matrix, Nanoparticle, Cotton textiles, 02 engineering and technology, engineering.material, 010402 general chemistry, 01 natural sciences, Article, Antibacterial properties, lcsh:Chemistry, chemistry.chemical_compound, Coating, Iridium (III) luminescent complexe, General Materials Science, Tetrazole, Cotton textile, Ceramic, Detection limit, Singlet oxygen, Cationic polymerization, Iridium (III) luminescent complexes, Antibacterial propertie, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, lcsh:QD1-999, visual_art, visual_art.visual_art_medium, engineering, Inductively coupled plasma, 0210 nano-technology, Luminescence, Nuclear chemistry
Abstract

This study was aimed at the production and characterization of coated cotton textiles with luminescent ceramic nanophases doped with cationic Ir(III) tetrazole complexes. We confirmed that SiO2 nanoparticles (NPs) do not affect the phosphorescent properties of the complexes that maintain their emission (610 and 490 nm). For the first time we transferred the luminescence feature from nanosol to textile surface, highlighting the advantages of using nanosilica as an encapsulating and stabilizing matrix. The optimized Ir@SiO2 suspensions were homogenously applied onto the cotton surface by dip-pad-dry-cure technique, as proved by the 2p-fluorescence microscope analysis. Once we verified the self-marker properties of the Ir(III) complex, we observed an excellent washing fastness of the coating with a very limited release. SiO2 in the washing water was quantified at maximum around 1.5 wt% and Ir below the inductively coupled plasma optical emission spectrometry (ICP-OES) detection limit of 1 ppm. A Franz cell test was used to evaluate any possible ex-vivo uptake of Ir@SiO2 nanoparticles across human skin tissues, showing that epidermis and dermis stop over 99% of Ir, implying a reduced impact on human health. The light-induced antimicrobial potential of the Ir@SiO2 were assessed toward both Gram(&minus, ) and Gram(+) bacteria. The results encouraged further developments of such functional textiles coated by self-markers and antibacterial active nanophases.

Published
2020
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48. New examples of Ru(<scp>ii</scp>)-tetrazolato complexes as thiocyanate-free sensitizers for dye-sensitized solar cells

Author

Edoardo Marchini, Loris Giorgini, Mattia Averardi, Nicola Sangiorgi, Alessandra Sanson, Valentina Fiorini, Sara Muzzioli, Stefano Stagni, Roberto Argazzi, Angela Dellai, Stefano Caramori, Fiorini V., Marchini E., Averardi M., Giorgini L., Muzzioli S., Dellai A., Argazzi R., Sanson A., Sangiorgi N., Caramori S., and Stagni S.

Subjects
chemistry.chemical_classification, Ruthenium DSSC Tetrazoles Thiocyanate free, EFFICIENCY, Absorption spectroscopy, Thiocyanate, POTENTIALS, Ambientale, Time-dependent density functional theory, PE4_15, Inorganic Chemistry, CN, Dye-sensitized solar cell, chemistry.chemical_compound, LIGHT, Deprotonation, Dicarboxylic acid, chemistry, ELECTRON-TRANSFER, TIO2, INJECTION, ACID, Polymer chemistry, Molecule, Chelation
Abstract

A set of three new Ru(ii) polypyridyl complexes decorated with 5-aryl tetrazolato ligands (R-CN4)-, (D series, namely D1, D3 and D4), is presented herein. Whereas complex D1 represents the pyrazinyl tetrazolato analogue of a previously reported Ru(ii) complex (D2) with the general formula cis-[(dcbpy)2Ru(N^N)]+, in which dcbpy is 2,2′-bipyridine-4,4′-dicarboxylic acid and N^N is the chelating 2-pyridyl tetrazolato anion, the design of the unprecedented Ru(ii) species D3 and D4 relied upon a completely different architecture. More specifically, the molecular structure of thiocyanate-based species cis-[(dcbpy)2Ru(NCS)2], that is typically found in benchmark Ru(ii) dyes for dye sensitized solar cell (DSSCs), was modified with the replacement of two of the -NCS ligands in favour of the introduction of 5-aryl tetrazolato anions, such as the deprotonated form of 5-(4-bromophenyl)-1H-tetrazole, for complex D3 and 5-(4-cyanophenyl)-1H-tetrazole in the case of complex D4. To streamline the behavior of the D series of Ru(ii) complexes as photosensitizers for DSSCs, an in-depth analysis of the excited state properties of D1, D3 and D4 was performed through TDDFT calculations and TDAS (nanosecond transient difference absorption spectroscopy). The obtained results highlight a trend that was confirmed once D1, D3 and D4 were tested as photosensitizers for DSSC under different conditions. Along the series of the Ru(ii) complexes, the neutrally charged species D3 and D4 displayed the best photovoltaic performances. This journal is

Published
2020
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49. Antibacterial activity of a new class of tris homoleptic Ru (II)-complexes with alkyl-tetrazoles as diimine-type ligands

Author

Nicola Monti, Massimiliano Massi, Stefano Zacchini, Alejandro Hochkoeppler, Alessandra Stefan, Loris Giorgini, Stefano Stagni, Valentina Fiorini, Monti N., Zacchini S., Massi M., Hochkoeppler A., Giorgini L., Fiorini V., Stefan A., and Stagni S.

Subjects
Tris, chemistry.chemical_classification, Deinococcus radioduran, biology, Gram-positive bacteria, Tetrazoles, Deinococcus radiodurans, General Chemistry, biology.organism_classification, Medicinal chemistry, Inorganic Chemistry, Antibacterial, chemistry.chemical_compound, chemistry, gram-positive bacteria, ruthenium (II) complexe, Homoleptic, Antibacterial activity, Diimine, Alkyl
Abstract

Herein, we describe a new family of tris chelate homoleptic Ru (II) complexes, [Ru(N^N)3]2+, where the role of the diimine-type ligands (N^N) was fulfilled by 2-pyridyl (PTZ) or 2-quinolyl tetrazole (QTZ) derivatives decorated with various alkyl substituents at the N-2 position of the tetrazole ring. The new Ru (II) complexes with general formula [Ru (PTZ-R)3]2+ and [Ru (QTZ-R)3]2+, were obtained as mixtures of facial (fac) and meridional (mer) isomers, as suggested by NMR (1H, 13C) experiments, and confirmed in the case of mer-[Ru (QTZ-Me)3]2+, by X-ray crystallography. The photophysical behavior of the tetrazole-based [Ru(N^N)3]2+ type species was investigated by UV–vis absorption spectroscopy, providing trends typical of polypyridyl Ru (II) complexes. The new homoleptic complexes fac/mer-[Ru (PTZ-R)3]2+ and fac/mer-[Ru (QTZ-R)3]2+ have been assessed for any eventual antimicrobial activity towards two different bacteria such as Gram-negative Escherichia coli and Gram-positive Deinococcus radiodurans. Whereas being inactive toward E. coli, the response of agar disks diffusion tests suggested that some of the new fac/mer Ru (II) complexes could inhibit the growth of D. radiodurans. This effect was further investigated by determining the growth kinetics in liquid medium of D. radiodurans exposed to the fac/mer-[Ru (PTZ-R)3]2+ and fac/mer-[Ru (QTZ-R)3]2+ complexes at different concentrations. The outcome of these experiments highlighted that the turn-on of the growth inhibitory effect took place as the linear hexyl chain was appended to the PTZ or QTZ scaffold, suggesting also how the inhibitory activity appeared more pronouncedly exerted by the facial isomers fac-[Ru (PTZ-Hex)3]2+ and fac-[Ru (QTZ-Hex)3]2+ (MIC = ca. 3.0 μg/ml) with respect to the corresponding meridional isomers (MIC = ca. 6.0 μg/ml).

Published
2020

50. Complementary Approaches to Imaging Subcellular Lipid Architectures in Live Bacteria Using Phosphorescent Iridium Complexes and Raman Spectroscopy

Author

Anna Maria Ranieri, Massimiliano Massi, Stefano Stagni, Paul Rigby, Chiara Caporale, Mark J. Hackett, Valentina Fiorini, Alysia Hubbard, Elizabeth Watkin, Mark I. Ogden, Ranieri A.M., Caporale C., Fiorini V., Hubbard A., Rigby P., Stagni S., Watkin E., Ogden M.I., Hackett M.J., and Massi M.

Subjects
Confocal, Bacillus cereus, chemistry.chemical_element, 010402 general chemistry, Iridium, Spectrum Analysis, Raman, 01 natural sciences, Catalysis, Polyhydroxybutyrate, symbols.namesake, Coordination Complexes, Organelle, luminescence, Bacillus cereu, bacterial imaging, Luminescent Agent, Luminescent Agents, Microscopy, Confocal, biology, Coordination Complexe, 010405 organic chemistry, Organic Chemistry, General Chemistry, Lipid, biology.organism_classification, Lipids, 0104 chemical sciences, Cereus, chemistry, Raman spectroscopy, symbols, Biophysics, Phosphorescence, imaging agent
Abstract

A family of three neutral iridium(III) tetrazolato complexes are investigated as bacterial imaging agents. The complexes offer a facile tuning of the emission colour from green (520 nm) to red (600 nm) in aqueous media, while keeping the excitation wavelength unchanged. The three complexes do not inhibit the bacterial growth of Bacillus Cereus, used as a model in this study, and exhibit extremely fast cellular uptake. After a minute incubation time, the nontoxic complexes show subcellular localisation in spherical structures identified as lipid vacuoles. Confocal Raman imaging has been exploited for the first time on live bacteria, to provide direct and label-free mapping of the lipid-enriched organelles within B. cereus, complementing the use of luminescent probes. Examination of the Raman spectra not only confirmed the presence of lipophilic inclusions in B. cereus but offered additional information about their chemical composition, suggesting that the lipid vacuoles may contain polyhydroxybutyrate (PHB).

Published
2019

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