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doi:10.1111/j.1741-2358.2009.00329.x Experiments on in vivo biofilm formation and in vitro adhesion of Candida species on polysiloxane liners Objectives: Microorganisms may colonise polysiloxane soft liners leading to bio-deterioration. The aim of this study was to investigate in vitro adhesion and in vivo biofilm formation of Candida species on polysiloxane surfaces. Methods: The materials used in this study were Molloplast B, GC Reline soft, Mollosil Plus, Silagum Comfort and Palapress Vario. The in vitro retention of clinical isolates of Candida albicans to the relining and denture-base materials by microscopic (scanning electron microscopy, SEM), conventional culturing methods and antimicrobial properties of these materials were studied. Candida found on materials and mucosa following long-term use were identified and quantified, and biofilms covering the surfaces were investigated by SEM. Results: There was a significant decrease in the number of cells attached in vitro to saliva-coated surfaces compared with non-treated surfaces. An oral Candida carriage of 78% was found. Candida albicans, C. glabrata, C. intermedia and C. tropicalis were identified. In vivo biofilm formation on the liners appeared as massive colonisation by microorganisms. Conclusions: The results of the in vitro experiments suggest that salivary film influences early colonisation of different C. albicans strains. The film layer also minimises the differences among different strains. The Candida carriage of these patients was similar to denture-wearing patients without soft liners.

An in vitro test is described that simulates the in vivo fate of a denture adhesive, namely destruction, dilution, and dissolution of the adhesive, by repeated measurement of tensile bond strength for the adhesive in isotonic saline over time. The bond strengths were measured under two different settings of the testing machine. The main compositions of three denture adhesive pastes, Fittydent, Fixodent, and Super Poli-Grip and one powder adhesive, Super Wernet's, were determined by infrared spectroscopy. The tensile bond strengths of these adhesives and those of pure tragacanth gum were subjected to a three-way analysis of variance. The Fittydent and Super Poli-Grip adhesives exhibited the best results over time. The most appropriate of the described methods for testing denture adhesives seem useful; however, when seen in relation to the results of a published clinical study where the patients were asked to evaluate the retention and the duration of some of these adhesives, there are indications that the clinical validity of the method might be improved if paste adhesives are tested at temperatures above 35 degrees C.

Microorganisms may colonise polysiloxane soft liners leading to bio-deterioration. The aim of this study was to investigate in vitro adhesion and in vivo biofilm formation of Candida species on polysiloxane surfaces.The materials used in this study were Molloplast B, GC Reline soft, Mollosil Plus, Silagum Comfort and Palapress Vario. The in vitro retention of clinical isolates of Candida albicans to the relining and denture-base materials by microscopic (scanning electron microscopy, SEM), conventional culturing methods and antimicrobial properties of these materials were studied. Candida found on materials and mucosa following long-term use were identified and quantified, and biofilms covering the surfaces were investigated by SEM.There was a significant decrease in the number of cells attached in vitro to saliva-coated surfaces compared with non-treated surfaces. An oral Candida carriage of 78% was found. Candida albicans, C. glabrata, C. intermedia and C. tropicalis were identified. In vivo biofilm formation on the liners appeared as massive colonisation by microorganisms.The results of the in vitro experiments suggest that salivary film influences early colonisation of different C. albicans strains. The film layer also minimises the differences among different strains. The Candida carriage of these patients was similar to denture-wearing patients without soft liners.

The characteristics of the masseter reflex evoked by tapping a maxillary incisor were compared with the reflex pattern evoked by tapping a corresponding denture tooth after insertion of an immediate denture. Up to three inhibitory phases (I-1, I-2 and I-3), followed by excitation, were found on an averaged EMG. The tapping force threshold for the early inhibitory phase was lower than for the late phases. The pattern of the reflex was generally the same before and after insertion of the denture, but the threshold values increased. After insertion of the denture, the threshold for I-1 increased from 1 +/- 0.3N to 2.2 +/- 0.4N, the threshold for I-2 increased from 2.4 +/- 0.8N to 3.8 +/- 0.9N, and the threshold for I-3 increased from 5.1 +/- 0.6N to 8.3 +/- 0.9N. The latency period for I-1 also increased from 12.3 +/- 0.5 ms to 13.1 +/- 0.3 ms after insertion of the denture. After relining, the threshold for evoking I-1 decreased from 2.7 +/- 1.2N to 1.2 +/- 0.6N. It was assumed that the mechanoreceptors situated in the mucosa under the denture base could take over the functional role of the periodontal mechanoreceptors for evoking the masseter reflex during tapping, and that these afferents probably had connections to the same interneurones.

WOS: 000257977000016, PubMed ID: 18717174, Objective: The aim of the present study was to evaluate the clinical performance of four denture soft liners up to 12 months. Materials and methods: Thirty-three edentulous patients who experienced difficulties when using hard denture bases because of changes in denture-supporting tissues were accepted for the study and randomly received Molloplast B, GC Reline Soft, Silagum Comfort, or Mollosil Plus relines. Performance of the materials was evaluated using nine criteria at 3, 6, and 12 months: physical integrity, surface detail, adhesion, color, odor, plaque accumulation, resilience, hygiene, and mucosal condition. A four-point categorized scale (1=poor, 2=fair, 3=good, 4=excellent) was used. Unscheduled maintenance events and the presence of fungal colonization were also recorded. Results: The percentage of patients available at 3, 6, and 12 months were 91%, 91%, and 66%. Main reasons for dropouts and discontinuation were fractured dentures and patient dissatisfaction. At 6 months, 96% of the performance scores were good or excellent and the largest changes were observed for physical integrity, surface detail, color, and fungal colonization. Fungal colonization was the most commonly observed problem and was the only reason of failure at 12 months. Conclusions: The clinical performance of all soft liners was slightly impaired over the 12-month observation. Except for cases showing extensive fungal colonization, the observed changes in clinical performance did not necessitate remaking of the dentures. Mollosil Plus showed a performance comparable to that of Molloplast B, and the other materials had slightly lower performance especially in terms of fungal colonization.

There appears to be a relation between accumulation of plaque on complete maxillary dentures and denture stomatitis. The present index was introduced to assess the location and amount of plaque in some areas of the fitting surfaces of complete maxillary dentures. Scores were recorded in five defined areas on the denture base. Four ranked scores were used: 0 - no visible plaque, 1-plaque visible only by scraping on the denture base with a blunt instrument, 2-moderate accumulation of visible plaque, and 3-abundance of plaque. By summing up the scores from the five areas, the plaque was recorded on a scale from 0 to 15 points. The inter- and intra-reliability were tested for two examiners using the index on 50 complete maxillary dentures. Expressed in terms of Scott's Pi, inter-examiners agreement was 0.83, and intra-examiner 0.83 and 0.82 for examiners A and B, respectively. A simplification of the index was also presented. In this index the scores 0 and 1 were combined to score 0, -no visible plaque, and score 2 and 3 were combined to score 1, -visible plaque. Using this index, Scott's Pi was calculated to 0.91 for inter-examiner reliability. For the intra-examiners reliability Scott's Pi was calculated to 0.91 for examiner A and 0.89 for examiner B.

The maximal bite force was recorded between antagonizing anterior teeth in a group (n = 10) of patients with pathologic attrition and in a control group. No statistically significant difference between the maximal bite forces was recorded. Also, the effect on the maximal bite force of the continuous wearing of a partial bite raising splint for 4 months was recorded. The great individuality in reaction patterns prohibited simple generalizations. However, in some individuals the maximal bite force was larger when biting on the splint than without, both at base line and after 4 months. Also, the maximal bite force showed a tendency to increase with time. The findings indicate that pathologic attrition is not necessarily associated with a high maximal bite force. Also, the maximal bite force recorded under various conditions is seemingly not an absolute value but rather the result of a number of factors of varying importance for different individuals.

Complete maxillary dentures were tested for their ability to remain in place when subjected to unilateral occlusal loads. The test material comprised five persons, each supplied with three identical dentures. The denture design was based on the principles of 1) functionally determined filling-in of the vestibular sulcus, 2) palatal coverage to the vibration line without post dam, 3) aesthetically governed positioning of the front teeth, and 4) positioning of the lateral teeth in the plane connecting the top of the residual ridge with the central part of the occlusal surface of the antagonizing natural teeth. Resistance to unilateral occlusal loads was measured by means of a miniature bite force sensor. In the pooled material, an average load of 70 N was tolerated before the dentures were dislodged. For a given participant/denture combination, the resistance against dislodgment varied considerably when tested on different days. Marked differences were also found among three identical dentures in one person. The tolerance against unilateral occlusal loads could feasibly be quantified. However, the influence of specific clinical and/or technological factors on denture retention during function should be studied only if strict definitions as to the test conditions are given. These conditions must include the time, person, and denture tested.

The influence of free tongue, lip, and cheek function on the retention of complete maxillary dentures was investigated for five experienced denture wearers. Test dentures were designed with full palatal coverage and functionally determined filling in of the vestibular sulcus. The front teeth were arranged primarily to meet cosmetic demands--that is, anterior to the top of the residual alveolar ridge. Retention was measured as resistance to dislodgement-provoking loads applied vertically to the incisive edge of the central incisors, using a miniature bite force recorder. All the participants were able to load their front teeth with 35 N or more without loss of retention. None of them experienced denture dislodgement provided the tongue, lips, and cheeks wee allowed to act freely. If the peripheral soft tissues were separated from the vestibular denture flange, no obvious effect on denture retention could be detected. Physically preventing the tongue from pressing against the posterior part of the denture reduced the retention significantly. Measurements of anterior loads tolerated after stepwise reductions of the denture extension indicated that the tongue acted primarily by pressure against the tuber regions. Tongue pressure against the central parts of the palate and lip or cheek pressure against the vestibular flange seemed to be of less importance.

Maximal mouth opening, translatory condylar motion (radiographically assessed) and bite force in sixteen adults with rheumatoid arthritis and radiographic temporomandibular joint abnormalities were compared with data from sixteen individuals without joint disease. Mean mouth opening in the arthritis group was reduced, but not statistically significant. Condylar translation and bite force showed significantly lower values than in the healthy individuals. Maximal mouth opening seemed to be the most uncertain parameter for the estimation of jaw function. In seven patients with a normal mouth opening (40-46 mm), both the condylar translation and the bite force were significantly impaired.

Oral conditions were studied clinically and radiographically in a group of people aged 67-94 years. The group comprised 70 persons living in an institution for elderly people and 111 persons living in their homes in the surrounding area. The participants were classified according to sex, age and former occupation. The examination was carried out in a dental office established at the institution. 63% of the participants were dentulous with a mean number of 13 teeth. Almost 50% of the teeth were previously restored and 13% of the teeth were decayed at the examination. 45% had complete maxillary dentures and 39% had complete mandibular dentures. The need for dental treatment according to defined criteria was registered, and treatment-offers were given. Comparisons were made between the treatment suggested and the treatment actually given. Of the total group, 129 persons needed dental treatment. Of these, 65 persons accepted all or part of the treatment suggested. In general, sex was not a good predictor of the need for an acceptance of dental services. However, age was found to be useful as a predictor. Social background was an unreliable predictor of the need for and acceptance of dental services.

Twenty patients with complete dentures in both jaws gave up their maxillary ones for experimental purposes for 4 weeks while new dentures were made. The experimental dentures were reduced palatally to a new U-shaped border situated approximately 10 mm from the dental arch. Denture retention, defined as the ability of a denture to remain seated on the supporting tissues under various conditions, was measured as resistance towards cranially directed tilting loads, using a miniature bite force recorder. Dislodgement-provoking loads were applied to the first bicuspid on both sides and to the central incisors. The retention measurements were performed before and immediately after reduction of the palatal coverage and, finally, after 4 weeks. Interview questions were posed concerning the subjects' experience of denture retention and some other aspects of oral comfort. The interviews were carried out before reduction of the palatal coverage and after 2 and 4 weeks. The results of the experimental dislodgement tests suggested that the ability to withstand tilting loads was insignificantly altered by reduction of the palatal coverage. This finding was in agreement with responses indicating that retention during meals remained unchanged. However, several individuals reported that denture retention between meals was impaired. Three denture wearers reported that reduction of the palatal coverage influenced their sense of taste positively, and one reported that an occasional gagging tendency had disappeared. At the end of the experiment the participants were given the choice of a new conventional denture or a new denture without palatal coverage.(ABSTRACT TRUNCATED AT 250 WORDS)

– In order to obtain a replica technique suitable for a complete or segments of a dental arch two different impression materials were combined with two epoxy resin model materials. The models were examined and photographed in an optical microscope and a scanning electron microscope. No systematic differences were found between the used combinations of materials except a tendency towards greater contraction of one of the model materials. The A-silicone impression materials combined with one of the epoxy resin material seemed to be the easiest to handle and to give the best models.