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Proton therapy can treat tumors located in radiation-sensitive tissues. This article demonstrates the possibility of enhancing the proton therapy with targeted gold nanoparticles that selectively recognize tumor cells. Au-PEG nanoparticles at concentrations above 25 mg/L and 4 Gy proton dose caused complete death of EMT6/P cells in vitro. Binary proton therapy using targeted Au-PEG-FA nanoparticles caused an 80% tumor growth inhibition effect in vivo. The use of targeted gold nanoparticles is promising for enhancing the proton irradiation effect on tumor cells and requires further research to increase the therapeutic index of the approach. [ABSTRACT FROM AUTHOR]

In this article, we discuss the results of the study of ash heaps typical for the steppe belt of the Urals, Kazakhstan and Siberia. These are specific objects adjacent to settlements of the Middle and Late Bronze Age, their cultural layer contains archaeological finds and consists of loose soil similar in appearance to ash. However, debates about their nature and purpose use mainly archaeological arguments and very rarely involve interdisciplinary data. The study is focused on two ash heaps at the Bronze Age settlements in the Southern Trans-Urals — Streletskoye-1 and Chernorechye 2 (Chelyabinsk Oblast, Russia). Both are located on the bank cliff, between the dwelling pits and the river; the time of their existence mainly belongs to the Alakul archaeological culture (18th–16th centuries BC). The stratigraphy and granulometric composition of soil samples, composition of archaeobotanical remains, palynological, microbiomorphic, and soil-microbiological analyses, and assessment of biomarkers content have been carried out. It has been concluded that, according to a number of characteristics, ash heaps have a non-uniform nature of formation and differ both between themselves and in comparison with the previously studied ash heap at the fortified settlement of Stepnoye. The lower part of the ash heap of Streletskoye-1 is the soil accumulated during digging of semi-dwellings. The main volume of ash heaps layers was the result of plant biomass decomposition, but there are also markers of animal origin — keratin, cholesterol. No signs of manure were found in the samples. The use of ash and combustion products has not been confirmed at these newly examined sites, as well as at the ash heap of Stepnoye. Both ash heaps were formed in more hydromorphic conditions than the Stepnoye. Taking into account the archaeological context of the ash heaps, it has been suggested that in ancient times these structures near settlements were mainly used to store hay for livestock, and occasionally as a place for butchering animal carcasses and processing bones for bone-carving. The wintering of livestock, some of which were kept in settlements, created the need for fodder to keep the animals alive. Despite many supplementary functions of ash heaps, which were used as working or dumping areas, this is perhaps the first time in the archaeological record that evidence has been found for haymaking and hay storage in the vicinity of pastoral settlements. Interdisciplinary research on the properties of ash heaps is a relatively new field, but it has already yielded interesting results that allow reasoned assumptions to be made about the construction and function of these sites.

An ash heap is defined as a specific object at an archaeological site, usually in the form of a separate hillock containing a layer of finely-dispersed greyish-yellow or ash-coloured soil. The existing assumptions about the formation and functional purpose of ash heaps, as special archaeological objects found near the Bronze Age pastoral settlements, have so far been poorly supported by scientific data. At the ash heap near the Sintashta fortified settlement of Stepnoye, Chelyabinsk Oblast, for the first time, large-scale and comprehensive studies were carried out on the structure of these deposits, their granulometric and geochemical composition, in combination with the analysis of plant macroremains, paleoparasites, microbiomorphic and palynological complexes, indicative bacterial groups, and keratinophilic fungi. Judging by the archaeological materials, it is patently obvious that this was a multifunctional object that developed over many centuries and played different roles at different stages. The results of the conducted analyses showed that the 45 cm thick “ash” horizon in the Stepnoye ash heap contains a significant proportion of a fine sand fraction, and not a silty fraction. An increase in the biophilic elements (predominantly calcium, as well as phosphorus and sulphur), accumulated mainly in the organic matter of faunal origin, was noted in the upper bench of the ash layer. However, the high concentration of phytoliths in the “ashy” deposits indicates that the layer was formed mainly as a result of the accumulation of a large amount of plant organic matter. The absence of charcoal and the scarcity of charred macroremains indicate that the deposited organic material was not burnt but decomposed naturally. The increased content of saprotrophic bacteria in the “ashy” layer also supports the natural decomposition of an organic matter. The composition of the phyto-lithic and pollen spectrum of the “ashy” horizon is not typical for the steppe of the south of Western Siberia, since it is dominated by a group of mixed herbs with elements of a ruderal flora, and not by cereals. All the data supports the hypothesis that the ash heap is the result of regular practice of cleaning of the floor and interiors of the buildings of the fortified settlement of Stepnoye. This is evidenced by the increase in the number of keratinophilic fungi, which decompose wool and hair and therefore accumulate in the occupational layer of the structures. Based on the results of the study, the hypotheses that the Stepnoye ash heap is a place of consolidated ash removal or composting of stable manure were rejected.

In the paper, the results of an archaeoparasitological analysis of a population group from the ancient Greek city of Pantikapaion, located in the historical centre of the modern city of Kerch (Republic of Crimea), are presented. The aim of the study is to obtain information about the parasitic diseases, state of health, nutrition, and hygiene in a part of the population of the city. The materials for the study were soil samples obtained during the treatment of the human sacra from 31 burials of the necropolis, dated to the 2nd century AD. In the result of the conducted investigation, the parasitic spectrum of the studied population group was determined. Eggs of three species of helminths were found. The discovery of eggs of broad tapeworm (Dibothriocephalus latum) in the soil samples indicates the presence in the diet of the studied population group freshwater fish that were not sufficiently thermally treated. The archaeoparasitological data from archaeological sites of a chronologically close period in the territory of the Northern Black Sea region suggests that diphyllobothriasis was a decease far from being rare in this area. The presence of eggs of human roundworm (Ascaris lumbricoides) and whipworm (Trichuris trichiura) in the samples likely indicates the challenging sanitary-hygienic environment in the city and insufficient hygienic skills in the studied group. The analysis of the archaeoparasitological data within the historical context and utilisation of the archaeopathological material from the archaeological sites in the Northern Black Sea region permitted to identify the factors that had an influence on the wide spread of geohelminths. The humid and warm climate facilitated maturation of geohelminth eggs in the soil, while the human activities concerned with the irrigation of the territory, building public water collection points, and waste buildup in the streets, in combination with the high population density, were causing the rise of the infectious hazard. The relatively high incidence rates of Trichuris trichiura and Ascaris lumbricoides may indicate a high prevalence of certain bacterial intestinal infections transmitted by the identical route. The occurrence of geohelminths, alongside the archaeological data, can indicate a low social status of the studied population group.