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20 results on '"Figueiredo, José Edson Fontes"'

2. Does sorghum phenolic extract have antifungal effect?

Author

da Conceição, Renata Regina Pereira, Queiroz, Valéria Aparecida Vieira, Simeone, Maria Lúcia Ferreira, da Silva Araújo, Dagma Dionísia, do Carmo, Paulo Henrique Fonseca, de Menezes, Cícero Beserra, Figueiredo, José Edson Fontes, and de Resende Stoianoff, Maria Aparecida

Published
2024
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8. CHARACTERIZATION OF FORAGE, SWEET AND BIOMASS SORGHUM FOR AGRONOMIC PERFORMANCE AND ENSILABILITY

Author

BORTOLUCCI DA ROSA, MARIA ANTONIA, primary, TARDIN, FLÁVIO DESSAUNE, additional, SOUZA, JULIANA MARIA DA SILVA, additional, DOS SANTOS, JANAINE APARECIDA POLLI, additional, MACEDO, TAINARA DE FREITAS, additional, SANTOS, JÉSSICA, additional, FREITAS, MURILO HENRIQUE DE, additional, TODESCATTO, FELIPE, additional, DA COSTA PARRELLA, RAFAEL AUGUSTO, additional, FIGUEIREDO, JOSÉ EDSON FONTES, additional, NETO, ARTHUR BEHLING, additional, and PEREIRA, DALTON HENRIQUE, additional

Published
2022
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10. Antifungal activity of bacterial strains from maize silks against Fusarium verticillioides

Author

de Fátima Dias Diniz, Gisele, primary, Cota, Luciano Viana, additional, Figueiredo, José Edson Fontes, additional, Aguiar, Frederick Mendes, additional, da Silva, Dagma Dionísia, additional, de Paula Lana, Ubiraci Gomes, additional, dos Santos, Vera Lúcia, additional, Marriel, Ivanildo Evódio, additional, and de Oliveira-Paiva, Christiane Abreu, additional

Published
2021
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13. Yield losses in off-season corn crop due to stalk rot disease

Author

Costa, Rodrigo Véras da, primary, Simon, Jones, additional, Cota, Luciano Viana, additional, Silva, Dagma Dionísia da, additional, Almeida, Rodrigo Estevam Munhoz de, additional, Lanza, Fabrício Eustáquio, additional, Lago, Bruno Cocco, additional, Pereira, Alan Alves, additional, Campos, Leonardo José Motta, additional, and Figueiredo, José Edson Fontes, additional

Published
2019
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17. Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)

Author

Figueiredo, José Edson Fontes, Gomes, Eliane Aparecida, Guimarães, Claudia Teixeira, Lana, Ubiraci Gomes de Paula, Teixeira, Marta Aparecida, Lima, Guilherme Vitor Corrêa, and Bressan, Wellington

Subjects
endophytic bacteria, Bactéria endofítica, Bacillus, sweet corn, milho doce, seqüenciamento de rDNA, rDNA sequencing, SDS-PAGE
Abstract

Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of wholecell protein extract of fortytwo isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing, Bacillus subitilis and B. pumilus were the most frequenty species (15 and 12 isolates, respectively) followed by B. licheniformes (7 isolates), B. cereus (5 isolates) and B. amiloliquefascens (3 isolates). According to present results, SDS-PAGE technique could be used as a fast and cheap first tool for identifying interspecific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intraspecific variation among isolates with similar protein profile as well as for taxonomic studies. Bactérias endofíticas desempenham papel importante na agricultura, melhorando a performance e adaptação de plantas contra estresses bióticos e abióticos. No presente estudo, métodos moleculares foram empregados para identificar bactérias endofíticas do gênero Bacillus isoladas de cultivares de milho doce brasileiro. SDS-PAGE de extratos protéicos totais de quarenta e dois isolados revelaram elevado número de bandas escrutináveis. Vinte e quatro isolados formaram nove grupos diferentes de réplicas bactérianas e dezoito foram considerados como únicos. Entre os isolados, alguns polipeptídios, de tamanhos variados, foram altamente acumulados. Seqüenciamento parcial do gene ribosomal 16S revelou que todos os isolados pertencem ao gênero Bacillus e que, entre treze isolados com padrão protéico similar, dois eram linhagens diferentes. Entre os quarenta e dois isolados identificados por seqüenciamento de rDNA, Bacillus subtilis e B. pumilus foram mais frequentes (15 e 12 isolados, respectivamente), seguido por, B. licheniformes (7 isolados), B. cereus (5 isolados) e B. amiloliquefascens (3 isolados). Baseado nos resultados, conclui-se que a técnica de SDS-PAGE poderá ser usada como primeiro procedimento, rápido e barato, para identificar variação inter-específica em coleções de bactérias endofíticas isoladas do milho, enquanto o método de seqüenciamento de rDNA poderá ser aplicado para analisar variações intra-específica entre isolados com padões similares de proteínas e estudos de taxonomia.

Published
2009

20. Chemical and genetic characterization of lipopeptides from Bacillus velezensis and Paenibacillus ottowii with activity against Fusarium verticillioides .

Author

Diniz GFD, Figueiredo JEF, Canuto KM, Cota LV, Souza ASQ, Simeone MLF, Tinoco SMS, Ribeiro PRV, Ferreira LVS, Marins MS, de Oliveira-Paiva CA, and Dos Santos VL

Abstract

Introduction: The fungus Fusarium verticillioides significantly threatens maize crops in tropical soils. In light of this, biological control has emerged as a promising strategy to reduce fungicide costs and environmental risks. In this study, we aimed to test the antifungal activity of cell-free supernatant (CFS) from three Bacillus velezensis (CT02, IM14, and LIS05) and one Paenibacillus ottowii (LIS04) against F. verticillioides , thereby contributing to the development of effective biocontrol measures., Methods: The research employed a comprehensive approach. The antifungal activity of the bacterial strains was tested using cell-free supernatant (CFS) from three Bacillus velezensis (CT02, IM14, and LIS05) and one Paenibacillus ottowii (LIS04). The UPLC-MS evaluated the CFS to identify the main bioactive molecules involved in the inhibitory effect on F . verticillioides . Scanning electron microscopy (SEM) was used to assess the impact of CFS on spores and hyphae, and genome sequencing was conducted to identify the genes involved in biological control. These robust methodologies ensure the reliability and validate our findings., Results: The CFS of the four strains demonstrated significant inhibition of fungal growth. The UPLC-MS analysis revealed the presence of lipopeptides with antifungal activity, including surfactin and fengycins A and B expressed by the three strains of Bacillus velezensis and iturin A expressed by strains LIS05 and IM14. For Paenibacillus ottowii , fusaricidins, ABCDE, and five previously unreported lipopeptides were detected. Scanning electron microscopy (SEM) showed that treatments with CFS led to significant distortion and breakage of the F . verticillioides hyphae, in addition to the formation of cavities in the membrane. Genome mining confirmed the presence of genes coding for the lipopeptides identified by UPLC-MS, including the gene for iturin in CTO2. Genomic sequencing revealed that CT02, IM14, and LIS05 belong to different strains of Bacillus velezensis , and LIS04 belongs to Paenibacillus ottowii , a species recently described., Discussion: The four bacterial strains, including three novel strains identified as Bacillus velezensis and one as the recently described species Paenibacillus ottowii , demonstrate significant potential as biocontrol agents for managing fungal disease. This finding underscores the novelty and potential impact of our research., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Diniz, Figueiredo, Canuto, Cota, Souza, Simeone, Tinoco, Ribeiro, Ferreira, Marins, Oliveira-Paiva and dos Santos.)

Published
2024
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