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Though correspondence across the affective experience and physiologic arousal levels of the stress response is thought to support efficacious coping and buffer against internalizing problems, little evidence has demonstrated such correspondence. Using a community sample of preadolescents (N=151, Mage=10.33 years, Minage=8.92, Maxage=12.00, 51.7% male), this person-centered study examined internalizing problem and coping-linked variability in psychobiological stress response correspondence. Preadolescents were exposed to the Trier Social Stress Test and self-reported negative affect (NA) and salivary cortisol (SC) levels were assessed. Multitrajectory modeling revealed four subgroups. Relative to In-Touch (i.e., Moderate NA-Moderate SC; n=65), Unmindful (i.e., Moderate NA-Low SC; n=49) were more likely to present with parent-reported but not self-reported internalizing problems; Vigilant (i.e., High NA-Low SC; n=13) were more likely to present with self- and parent-reported internalizing problems, less likely to use engagement coping, and more likely to use wishful thinking (e.g., "I wish problems would just go away."); Denial (i.e., Low NA-High SC; n=24) self-reported similarly low internalizing problems, but were also more likely to report reliance on denial (e.g., "I pretend problems don't exist."). Findings illustrate meaningful heterogeneity in preadolescent psychobiological correspondence with implications for multimodal assessment and outcome monitoring in coping-based preventative interventions.

OBJECTIVE The primary aims of this study were to identify latent profiles of acute stress disorder (ASD) symptoms and to evaluate postconcussive symptom differences across the identified profiles as measured by the Acute Stress Disorder Scale and the Military Acute Concussion Evaluation, respectively. METHOD Participants (N = 315) in the current study were predominantly active-duty (75.0%), enlisted (97.8%) males (97.4%) serving in the U.S. Army (87.8%). Approximately, half of the sample reported being married or engaged (51.1%) and was on average 25.94 (SD = 6.31) years old. Participants were referred to the Air Force Theater Hospital, 332nd Air Expeditionary Wing, Joint Base Balad, Iraq, to be evaluated as part of routine clinical assessment for neurocognitive and psychological symptoms following exposure to a blast. RESULTS A 3-profile solution was identified as the most parsimonious and best-fitting model based on statistical model fit indices. Blast injured service members in Profile 3 had greater ASD total and subscale severity compared to the other 2 subgroups, with effect size estimates largely differing by hyperarousal and reexperiencing symptoms. Furthermore, Profiles 2 and 3 were more likely to demonstrate postconcussive symptoms compared to Profile 1. CONCLUSIONS Findings provide novel information on heterogenous ASD symptom profiles during the acute phase following a blast injury and highlight the relationship between psychological and physical symptoms. Classification of blast-injured service members may help identify at-risk individuals who would benefit from further clinical care and mitigate long-term psychological and neurocognitive issues. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

Nitrogen removal is a critical process in water treatment plants (WTPs) and wastewater treatment plants (WWTPs). The recent discovery of a novel bacterial process, complete ammonia oxidation (comammox, CMX), has refuted a century-long perception of the two-step conversion of NH3 to NO3–. Compared with canonical nitrifiers, CMX bacteria offer undeniable advantages, such as a high growth yield propensity and adaptability to nutrient- and growth-limiting conditions, which collectively draw attention to validate the aptness of CMX bacteria to wastewater treatment. As there has been no comprehensive review on the relevance of CMX bacteria for sustainable water and wastewater treatment, this review is intended to discuss the roles and applications of CMX in the removal of nitrogen and pollutants from water and wastewater. We took into account insights into the metabolic versatilities of CMX bacteria at the clade and subclade levels. We focused on the distribution of CMX bacteria in engineered systems, niche differentiation, co-occurrence and interactions with canonical nitrifiers for a better understanding of CMX bacteria in terms of their ecophysiology. Conceptualized details on the reactor adaptability and stress response of CMX bacteria are provided. The potential of CMX bacteria to degrade micropollutants either directly or co-metabolically was evaluated, and these insights would be an indispensable advantage in opening the doors for wider applications of CMX bacteria in WWTPs. Finally, we summarized future directions of research that are imperative in improving the understanding of CMX biology.

Integration is the process of combining parts to create a coordinated, or integrated, whole; it is found at all levels of organization of living matter. Every biological function is the expression of some sort of integration of parts, which allows the complexity of a system to grow and new properties to emerge. Keywords: cell junctions; desmosomes; synapses; central nervous system; autonomic nervous system; fight-or-flight response; endocrine system; sympathetic nerves

Humidity is probably the most important abiotic factor influencing life cycles, distribution, survival, and population dynamics of stored product pests. Although most of these pests can complete their life cycles in any given relative humidity, their prolonged development time, as well as decreased emergence rate and fecundity, have been well documented in several previous studies. In the present study, we evaluated the changes in energetic substances (lipids, soluble carbohydrates, glycogen, and proteins) accumulated in different life stages of larvae and adults of Tribolium castaneum in response to different relative humidity levels (5, 12, 22, 30, 45, and 65%). The results showed that young larvae were more susceptible to low relative humidity levels and desiccation stress. Larvae tended to accumulate higher proportions of lipids during earlier stages while their energy content shifted towards proteins with an increase in their age. Adult beetles experienced a significant decrease in their protein content immediately after they initiated reproduction. The importance of these fluctuations in the biology of the red flour beetles was discussed in detail.

DNA methylation represents a major type of DNA modifications that play key roles in diverse biological processes. With the recent development of highly selective and sensitive bioanalytical techniques, N6-methyladenine (6mA) has been characterized as an important internal DNA modification dynamically occurring in multiple eukaryotes including humans. Increasing evidence has indicated that 6mA may act as a novel epigenetic modification involved in regulation of development, stress response and diseases such as cancer and neurodegenerative disorders. We review herein the recent advances in the detection and functional studies of 6mA modification, with special emphasis on its biological consequences and human health relevance as well as its dynamic regulation by various types of methyltransferases, demethylases and 6mA-binding proteins. It can be envisaged that further chemical and biological studies of 6mA modification will lead to a better understanding about its potentially important roles in normal and pathological biological processes.

This review investigates the effects of psychological stress on the human body measured through biosignals. When a potentially threatening stimulus is perceived, a cascade of physiological processes occurs mobilizing the body and nervous system to confront the imminent threat and ensure effective adaptation. Biosignals that can be measured reliably in relation to such stressors include physiological (EEG, ECG, EDA, EMG) and physical measures (respiratory rate, speech, skin temperature, pupil size, eye activity). A fundamental objective in this area of psychophysiological research is to establish reliable biosignal indices that reveal the underlying physiological mechanisms of the stress response. Motivated by the lack of comprehensive guidelines on the relationship between the multitude of biosignal features used in the literature and their corresponding behaviour during stress, in this paper, the impact of stress to multiple bodily responses is surveyed. Emphasis is put on the efficiency, robustness and consistency of biosignal data features across the current state of knowledge in stress detection. It is also explored multimodal biosignal analysis and modelling methods for deriving accurate stress correlates. This paper aims to provide a comprehensive review on biosignal patterns caused during stress conditions and reliable practical guidelines towards more efficient detection of stress.

Diabetes mellitus and associated chronic hyperglycemia enhance the risk of acute ischemic stroke and lead to worsened clinical outcome and increased mortality. However, post-stroke hyperglycemia is also present in a number of non-diabetic patients after acute ischemic stroke, presumably as a stress response. The aim of this review is to summarize the main effects of hyperglycemia when associated to ischemic injury in acute stroke patients, highlighting the clinical and neurological outcomes in these conditions and after the administration of the currently approved pharmacological treatment, i.e. insulin. The disappointing results of the clinical trials on insulin (including the hypoglycemic events) demand a change of strategy based on more focused therapies. Starting from the comprehensive evaluation of the physiopathological alterations occurring in the ischemic brain during hyperglycemic conditions, the effects of various classes of glucose-lowering drugs are reviewed, such as glucose-like peptide-1 receptor agonists, DPP-4 inhibitors and sodium glucose cotransporter 2 inhibitors, in the perspective of overcoming the up-to-date limitations and of evaluating the effectiveness of new potential therapeutic strategies.

By characterizing physiological changes that occur in warfighters during simulated combat, we can start to unravel the key biomolecular components that are linked to physical and cognitive performance. Viable field-based sensors for the warfighter must be rapid and noninvasive. In an effort to facilitate this, we applied a multiomics pipeline to characterize the stress response in the saliva of warfighters to correlate biomolecular changes with overall performance and health. In this study, two different stress models were observed - one of chronic stress and one of acute stress. In both models, significant perturbations in the immune, metabolic, and protein manufacturing/processing systems were observed. However, when differentiating between stress models, specific metabolites associated with the "fight or flight" response and protein folding were seen to be discriminate of the acute stress model.

Ribosomes are molecular machines that translate the genome into protein. They consume a large amount of energy, linking ribosomes to cellular energy management. This is one facet of ribosome stress, which we describe as a two-way process. Ribosomes are regulated by stress signaling, such as energy status, but also sense stress and enact appropriate responses. Stress sensing starts at ribosomes synthesis, which is linked to cell fate by ribosome components and biogenesis factors. Importantly, the working ribosome also senses stress-inducing aberrations, and has mechanisms to reduce their likelihood. Finally, even ribosome degradation is a regulated part of the stress response.

Does unethical leader behaviour produce gendered responses? We study follower response to unethical leader behaviours of bribery and workplace harassment. Our findings, based on an experiment, sugg...

Fungi are recognized as key pathogens in immunocompromised patients. The invasive infection always remains a problem for clinicians due to high morbidity and mortality. The treatments of fungal infections are hampered by conventional drugs, which are associated with resistance. Drug resistance has become an important problem in a variety of infectious diseases. The rise in the incidence of fungal infections and drug resistance has intensified the need for alternative therapies that affect a new target. This new target must be a growth essential gene product like the stress pathway. It has been found that stress pathways can be a potential target in opportunistic fungal infection, which played an important role in the virulence of pathogens. It was helpful in protection from host defense, normal fungal growth, and antifungal drug resistance. The disruption of the pathway using alternative strategies (chemosensitization and photo-dynamics therapy) can be a novel approach in fighting fungal infections and for drug design.

A copper-containing antibiotic Bacteria require transition metal ions for biological processes and must also protect themselves against excess metal, which is toxic. Patteson et al . explored how the environmental bacterium Pseudomonas aeruginosa uses a five-enzyme pathway to synthesize a small-molecule complex, fluopsin C, which is built from cysteine and contains a copper ion. The biosynthesis involves unusual enzymatic transformations that convert cysteine to a thiohydroximate, two of which chelate a copper ion in the final natural product. Fluopsin C protects P. aeruginosa from excess copper and also acts as a broad-spectrum antibiotic against other bacteria. —VV

Background\ud Emerging evidence suggests that antenatal exposure to maternal stress signals affects the development of the infant stress response systems. Animal studies indicate that maternal sensitive caregiving can reverse some of these effects. However, the generalizability of these findings to humans is unknown. This study investigated the role of maternal caregiving in the association between multiple markers of maternal antenatal stress and infant stress regulation.\ud \ud Methods\ud The sample consisted of 94 mother-infant (N = 47 males, mean postnatal weeks = 12; SD = 1.84) dyads. Maternal levels of Interleukin-6, C-Reactive Protein (CRP), diurnal cortisol and alpha amylase, depressive and anxiety symptoms were assessed in late pregnancy (mean gestational age = 34.76; SD = 1.12), whereas postnatal symptomatology, caregiving, and infant cortisol response to the inoculation were evaluated at 3 months.\ud \ud Results\ud Hierarchical linear models (HLMs) showed a significant interaction between maternal antenatal cortisol, caregiving, and time on infant cortisol reactivity, while controlling for gender, maternal age, and postnatal depression. Specifically, higher levels of maternal antenatal cortisol were associated with greater cortisol response only among infants of less emotionally available mothers. All other markers of antenatal stress were not significantly associated with infant cortisol reactivity either independently or in interaction with maternal caregiving.\ud \ud Conclusions\ud Albeit preliminary, results provide the first evidence in humans that maternal sensitive caregiving may eliminate the association between antenatal maternal cortisol and infant cortisol regulation.

Drought stress constitutes one of the major constraints to agriculture all over the world, and its devastating effect is only expected to increase in the following years due to climate change. Concurrently, the increasing food demand in a steadily growing population requires a proportional increase in yield and crop production. In the past, research aimed to increase plant resilience to severe drought stress. However, this often resulted in stunted growth and reduced yield under favorable conditions or moderate drought. Nowadays, drought tolerance research aims to maintain plant growth and yield under drought conditions. Overall, recently deployed strategies to engineer drought tolerance in the lab can be classified into a 'growth-centered' strategy, which focuses on keeping growth unaffected by the drought stress, and a 'drought resilience without growth penalty' strategy, in which the main aim is still to boost drought resilience, while limiting the side effects on plant growth. In this review, we put the scope on these two strategies and some molecular players that were successfully engineered to generate drought-tolerant plants: abscisic acid, brassinosteroids, cytokinins, ethylene, ROS scavenging genes, strigolactones, and aquaporins. We discuss how these pathways participate in growth and stress response regulation under drought. Finally, we present an overview of the current insights and future perspectives in the development of new strategies to improve drought tolerance in the field.

In the arid/semi-arid regions of India, there are many legumes often growing in the wild like Prosopis cineraria, Cyamopsis tetragonoloba and Acacia senegal. These legumes accumulate useful metabolites, often as glycosides, such as flavonoids and isoflavonoids under conditions of stress like heat and low rainfall. When required, some of these metabolites are produced by the activity of glycoside hydrolase enzymes like β-glucosidase rather than de novo biosynthesis. Local human populations have been consuming food prepared from the edible portions of these plants since ancient tradition. However, studies on phytochemical-cum-metabolic aspects are surprisingly limited in these plants—these being important parameters for developing them into scientifically-validated holistic health foods. It is important that this task is carried out for indigenous plants with useful antioxidant-guided medicinal properties rather than always depending upon expensive and imported exotic foods. The current article highlights research insights and perspectives from our work on edible desert legumes as well as those of other international research groups active in this area, particularly pertaining to flavonoids and their metabolism in planta. A few possible research goals for legume plant scientists are also suggested.

Background The spread of spinal anesthesia was influenced by many factors, and the effect of body height on spinal anesthesia is still arguable. This study aimed to explore the impact of height on the spread of spinal anesthesia and the stress response in parturients. Methods A total of ninety-seven parturients were allocated into two groups according to their height: the shorter group (body height was shorter than 158 cm) and taller group (body height was taller than 165 cm). Spinal anesthesia was performed with the same amount of 12 mg plain ropivacaine in mothers of different heights. The primary outcome of the study was the success or failure of the spinal anesthesia. The secondary outcomes of the study were stress response, time to T6 sensory level, the incidence of hypotension, the satisfaction of abdominal muscle relaxation and patient VAS scores. Results The rate of successful spinal anesthesia in the shorter group was significantly higher than that in the taller group (p = 0.02). The increase of maternal cortisol level in the shorter group was lower than that in the taller group at skin closure (p = 0.001). The incidence of hypotension (p = 0.013), time to T6 sensory block (p = 0.005), the quality of abdominal muscle relaxation (p p p p = 0.022), amniotic fluid index (p = 0.022) were significantly associated with successful spinal anesthesia. Conclusions It’s difficult to use a single factor to predict the spread of spinal anesthesia. Patient’s vertebral column length, amniotic fluid index and abdominal girth were the high determinant factors for predicting the spread of spinal anesthesia. Trials registration ChiCTR-ROC-17012030 (Chictr.org.cn), registered on 18/07/2017.

Environmental and/or occupational exposure to metals such as Arsenic (As), Cadmium (Cd), and Chromium (Cr) have been shown to induce carcinogenesis in various organs, including the urogenital system. However, the mechanisms responsible for metal-induced carcinogenesis remain elusive. We and others have shown that metals are potent inducers of autophagy, which has been suggested to be an adaptive stress response to allow metal-exposed cells to survive in hostile environments. Albeit few, recent experimental studies have shown that As and Cd promote tumorigenesis via autophagy and that inhibition of autophagic signaling suppressed metal-induced carcinogenesis. In light of the newly emerging role of autophagic involvement in metal-induced carcinogenesis, the present review focuses explicitly on the mechanistic role of autophagy and potential signaling pathways involved in As-, Cd-, and Cr-induced urogenital carcinogenesis.

Previous studies on the association of insomnia with body mass index (BMI) have been controversial. Physiological hyperarousal, the key pathological mechanism of insomnia, may be an important reason for different findings. We explored whether insomnia with physiological hyperarousal measured by the multiple sleep latency test (MSLT) is associated with body-weight differences. A total of 185 normal sleepers and 440 insomniacs were included in this study. Insomnia was defined by standard diagnostic criteria with symptoms lasting ≥6 months. All subjects underwent one night of laboratory polysomnography followed by a standard MSLT. We used the median MSLT value (i.e., ≥14 min) to define physiological hyperarousal. BMI was based on measured height (cm) and weight (kg) during the subjects’ sleep laboratory visit. BMI > 25 kg/m2 was defined as overweight, while BMI 2 was defined as underweight. After controlling for confounders, the odds of lower weight rather than overweight were significantly increased among insomnia patients with increased MSLT: insomnia with MSLT 14–17 min and MSLT > 17 min increased the odds of lower weight by approximately 89% (OR = 1.89, 95% CI 1.00–4.85) and 273% (OR = 3.73, 95% CI 1.51–9.22) compared with normal sleepers, respectively. In contrast, insomnia in patients with MSLT 11–14 min and 8–11 min was not different from normal sleepers in terms of body weight. Insomnia associated with physiological hyperarousal, the most severe phenotype of chronic insomnia, is associated with higher odds of lower weight and underweight compared with normal sleepers. This is a novel finding consistent with previous physiologic data and has significant clinical implications.

L’activation de l’hypothalamus par des signaux inflammatoires et/ou de stress peut déclencher celle de l’axe HPA (hypothalamic-pituitary-adrenal axis), qui intègre l’hypothalamus, l’hypophyse et la glande surrénale. L’activation aiguë de l’axe HPA est fondamentale pour la réponse fight or flight (« combats ou fuis »). Elle permet de mobiliser un maximum d’énergie pour un effort, tout en effaçant la fatigue. En revanche, son activation chronique diminue l’efficacité musculaire et entraîne une fatigue chronique. On discutera dans cette partie de plusieurs points stratégiques à considérer pour tenter de comprendre et de traiter ensemble inflammation et fatigue chroniques.

The goals of this study were to examine the effect of stocking density on the stress response and disease susceptibility in juvenile rainbow trout (Oncorhynchus mykiss). Fish were sorted into one of 2 stocking densities (high density "HD", 20-40 kg/m3) or (low density, "LD", 4-8 kg/m3) and 3 stress indices (cortisol levels in serum and water, and neutrophil: lymphocyte (N:L) ratios from blood smears) were measured at multiple time points over 21 d. Serum cortisol was significantly increased at 1 h in LD samples and at 14 d in HD samples. Water cortisol concentrations were significantly higher in LD tanks as compared with HD tanks on day 14. N:L ratios were significantly higher in HD tanks on day 14 as compared with LD tanks and with baseline. The effect of stocking density on mortality after exposure to infectious hematopoietic necrosis virus (IHNV) was compared between fish held in HD or LD conditions, with or without prior acclimation to the different density conditions. No significant differences in survival were found between HD and LD treatments or between acclimated and nonacclimated treatments. Cumulative results indicate that 1) 1 to 4 gram rainbow trout did not generally demonstrate significant differences in stress indices at the density conditions tested over a 21-d period, 2) independent differences were found in 3 stress indices at day 14 after sorting into LD and HD holding conditions; and 3) LD and HD stocking densities did not have a significant effect on mortality due to IHNV.

Background Postpartum dysgalactia syndrome (PDS) is associated with a significantly higher activation of the inflammatory and stress response at parturition than in the healthy sow. Therefore, reliable and possibly non-invasive biomarkers for substantial increases of inflammation are searched to support the PDS diagnosis. This report studies the possible changes of the inflammatory marker enzyme adenosine deaminase (ADA) in serum and saliva of 38 PDS positive sows (PDS+) and 38 healthy sows (PDS-). Sampling was performed every 24 h from 60 h before to 36 h after parturition. Isoenzyme 1 (ADA1) and isoenzyme 2 (ADA2), as well as total ADA (tADA), were measured and their statistical association with several serum and saliva biomarkers of inflammation and stress was investigated. Results Compared to a baseline (60 to 36h prepartum), salivary activities of ADA1, ADA2 and tADA increased significantly over time in both PDS+ and PDS- sows, reaching their peaks after parturition. In serum from PDS- sows, no changes were observed over time in either ADA1, ADA2 or tADA. In PDS+ sows, serum ADA2 activity decreased temporarily after parturition followed by a significant increase compared to baseline. ADA1, ADA2 and tADA were all significantly associated with several inflammatory biomarkers and ADA1 in serum was associated with serum cortisol. Although serum activity was higher in PDS+ than in PDS- sows, the differences were not statistically significant. Further, no difference was noted between the groups in the analyses of saliva. Conclusions Salivary ADA1 and ADA2 increased in all sows after parturition, potentially as a response to the postpartum inflammation. However, no difference in the activity of ADA1, ADA2 and tADA were found between PDS+ and PDS- sows indicating inability to diagnose PDS under the conditions described in this report.

Alcoholism is a serious problem that is difficult to treat. This study investigated the effects of group counseling utilizing narrative therapy on self-esteem, stress response, and insight for alco...

BACKGROUND The abnormal ultimate pH (pHu ) in postmortem muscles affect the meat quality and results in substantial economic losses. Dark, firm, and dry (DFD) meat linked with the higher postmortem pHu values and exhibited many quality issues such as dark color, tough texture and shorter shelf life. This research aimed to investigate the effect of protein phosphorylation on variations in beef pHu in order to explore the possible mechanisms underlying DFD meat formation. RESULTS Glycogen and lactate contents were higher, while L* and a* were lower in high pHu beef. Shear force was higher in intermediate pHu group. Global phosphorylation of sarcoplasmic proteins was higher in low pHu samples on day 1 and of myofibrillar proteins was higher in intermediate pHu meat on days 1 and 2 postmortem. Sarcoplasmic protein bands with different phosphorylation levels were identified as containing some glycometabolism and stress response proteins and phosphorylated myofibrillar protein bands were identified sarcomeric and metabolic proteins. CONCLUSIONS Phosphorylation of multiple proteins of glycolytic pathway and contractile machinery may play critical roles in development of DFD beef. © 2021 Society of Chemical Industry.

Background/aim To investigate the predictive effect of different symptoms of early acute stress disorder (ASD) on posttraumatic stress disorder (PTSD) in traffic accident survivors. Materials and methods A total of 206 traffic accident survivors were assessed with the Acute Stress Disorder Scale (ASDS) within 2-23 days after accidents, as well as with 17-item PTSD Checklist-Specific Stressor Version (PCL-S) during 4-12 months after accidents. All into the first group of subjects by senior surgeon assessment, based on the clinical, radiological and laboratory examination, excluded traumatic brain injuries and mild brain injury. And then, assessmented by clinical psychological practitioner. Results The severity of ASD can significantly predict the severity of PTSD symptoms. ASD reexperience symptoms and avoidance symptoms can significantly predict PTSD reexperience symptoms and avoidance symptoms. ASD hyperarousal symptoms can significantly predict PTSD hyperarousal symptoms. Conclusions ASD and PTSD are common psychological disorders among traffic accident survivors. ASD can predict the symptoms and severity of PTSD.

An analytical approach is constructed to examine the limit stresses of multilayered fiber reinforced disk. Three different layer configurations are considered, which are decreasing, constant and in...

The increasing number of people with hypertension worldwide has become a matter of grave concern. Blood pressure monitoring using a non-contact measurement technique is expected to detect and control this medical condition. Previous studies have estimated blood pressure variations following an acute stress response based on facial thermal images obtained from infrared thermography devices. However, a non-contact resting blood pressure estimation method is required because blood pressure is generally measured in the resting state without inducing acute stress. Day-long blood pressure variations include short-term variations due to acute stress and long-term variations in circadian rhythms. The aim of this study is to estimate resting blood pressure from facial thermal images by separating and excluding short-term variations related to acute stress. To achieve this, short-term blood pressure variations components related to acute stress on facial thermal images were separated using independent component analysis. Resting blood pressure was estimated with the extracted independent components excluding the short-term components using multiple regression analysis. The results show that the proposed approach can accurately estimate resting blood pressure from facial thermal images, with a 9.90 mmHg root mean square error. In addition, features related to resting blood pressure were represented in the nose, lip, and cheek regions.

Three Gram-staining negative, facultatively anaerobic, rod-shaped and motile strains, FJAT-51800T, FJAT-52962T and FJAT-54481T were isolated from the sediment samples of Zhangjiang Estuary Mangrove National Nature Reserve in Fujian Province, China. The 16S rRNA gene sequencing results indicated they could be novel members of the genus Shewanella. The optimum temperature for growth was 30 °C. The respiratory quinones of the strains were ubiquinone Q-7 or Q-8, and menaquinone MK-7. Polar lipids of the strains FJAT-52962T and FJAT-51800T were phosphatidyl glycerol, phosphatidyl ethanolamine, and unidentified aminophospholipids while strain FJAT-54481 consist of phosphatidylglycerol, phosphatidylethanolamine, unidentified aminophospholipids, two unidentified aminolipids and four unidentified lipids. The major fatty acid of the three strains was iso-C15:0. The genomic DNA G + C contents of strains FJAT-51800T, FJAT-52962T and FJAT-54481T were 48.2, 55.3 and 48.1%, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strains FJAT-51800T, FJAT-52962T and FJAT-54481T and other closely related Shewanella members were below the cut-off level (95–96%) for species identification. Genome analysis showed that these strains encode genes for osmo-regulation. Based on the results of phenotypic, chemotaxonomic and genome analyses, strains FJAT-51800T, FJAT-52962T and FJAT-54481T represent three novel species of the genus Shewanella, for which the names Shewanella avicenniae sp. nov., Shewanella sedimentimangrovi sp. nov., and Shewanella yunxiaonensis sp. nov., are proposed. The type strains are FJAT-51800T (= GDMCC 1.2204T = KCTC 82448T), FJAT-52962T (= MCCC 1K05496T = KCTC 82445T) and FJAT-54481T (= GDMCC 1.2348T = KCTC 82646T).

The sympathetic nervous system prepares the body for ‘fight or flight’ responses and maintains homeostasis during daily activities such as exercise, eating a meal or regulation of body temperature. Sympathetic regulation of bodily functions requires the establishment and refinement of anatomically and functionally precise connections between postganglionic sympathetic neurons and peripheral organs distributed widely throughout the body. Mechanistic studies of key events in the formation of postganglionic sympathetic neurons during embryonic and early postnatal life, including axon growth, target innervation, neuron survival, and dendrite growth and synapse formation, have advanced the understanding of how neuronal development is shaped by interactions with peripheral tissues and organs. Recent progress has also been made in identifying how the cellular and molecular diversity of sympathetic neurons is established to meet the functional demands of peripheral organs. In this Review, we summarize current knowledge of signalling pathways underlying the development of the sympathetic nervous system. These findings have implications for unravelling the contribution of sympathetic dysfunction stemming, in part, from developmental perturbations to the pathophysiology of peripheral neuropathies and cardiovascular and metabolic disorders. The sympathetic regulation of bodily functions relies on precise connections between sympathetic neurons and peripheral organs. In this Review, Scott-Solomon and colleagues discuss the mechanisms underlying the development of the sympathetic nervous system and provide insight into disorders regulated by this branch of the nervous system.

Clustered regularly interspaced short palindromic repeats‐CRISPR‐associated 9 (CRISPR‐Cas9) and base editors (BEs) are revolutionary gene‐editing technology that has been widely utilized in biology, biotechnology and medicine. However, recent reports show that CRISPR‐Cas9‐mediated genome editing can induce a p53‐mediated stress response and cell cycle arrest in human cells, while not illustrated in gene‐editing animals. In the study, to verify whether there is a phenomenon of p53 activation, by analysing nine gene‐edited rabbits using CRISPR‐Cas9 and BEs, we provide the first evidence that no apparent p53 expression changes in those rabbits generated by Cas9 or BE‐edited, suggesting that p53 may not need to consider for application in gene‐edited animals.

Purpose The aim of this study is to investigate the effects of preoperative oral intake of liquid carbohydrate on postoperative stress parameters (blood glucose, insulin resistance, cortisol, noradrenaline, and adrenaline levels) in patients who underwent laparoscopic cholecystectomy. Design This is an experimental study with intervention and control groups. Methods The sample consisted of 68 patients who underwent laparoscopic cholecystectomy (control group = 33; intervention group = 35). Twelve-hour preoperative fasting was applied to the patients in the control group in accordance with the clinical routine. Clear oral liquid carbohydrate (400 mL; 12.5 g/100 mL maltodextrin, 50 kcal/100 mL, pH 5.0) was administered to the patients in the intervention group at the preoperative second hour. Blood samples were taken from the patients at the preoperative 2nd and postoperative 2nd and 24th hours, and their blood glucose, insulin resistance, cortisol, noradrenaline, and adrenaline levels were measured. Results Preoperative oral intake of carbohydrate had no effect on blood glucose (P > .05) but decreased insulin resistance at the postoperative 24th hour (P = .044; intervention and control group: 3.62 ± 3.44 to 8.16 ± 12.57 respectively) and cortisol level at the postoperative 2nd hour (P = .005; intervention and control group: 15.16 ± 6.53 mg/dl to 20.14 ± 7.49 mg/dl, respectively). In all of the three measurements, we found that the noradrenaline level of the patients in the intervention group was higher than the value of those in the control group (319.80 ± 301.49 pg/mL to 211.65 ± 141.11 pg/mL [P = .450]; 361.40 ± 213.50 pg/mL to 216.13 ± 114.53 [P = .001]; 268.40 ± 164.04 pg/mL to 196.00 ± 83.33 pg/mL [P = .026], respectively). Preoperative oral intake of liquid carbohydrate had no effect on postoperative adrenaline level (P > .05). Conclusions Oral intake of liquid carbohydrate given at the preoperative 2nd hour decreased postoperative stress response through insulin resistance and cortisol.

The fracture response of macro polypropylene fiber reinforced concrete (PPFRC) and hybrid blend of macro and micro polypropylene fiber reinforced concrete (HyFRC) are evaluated at 1, 3, 7 and 28 days. There is an improvement in the early-age fracture response of HyFRC compared to PPFRC. The changing cohesive stress-crack separation relationship produced by ageing of the concrete matrix is determined from the fracture test responses. An improved early-age cohesive stress response is obtained from the hybrid blend containing micro and macro fibers. The hybrid fiber blend also has a higher tensile strength at early age when compared to an identical volume fraction of macro polypropylene fibers.

The aim of the present study is the first to evaluate the ecotoxic state of the marine environment in Anza-Taghazout coasts (Morocco) after installation of two Wastewater Treatment Plants using a natural population of marine bivalves M. galloprovincialis. These coasts are exposed to many discharges generating, thus, different sources of pollutants. These pollutants can modulate the physiological responses of marine bivalves to environmental stress. In this context, a multi-biomarker approach consisting of a battery of biomarkers evaluation was used to assess the response of these species to stress. In the whole soft tissues of M. galloprovincialis, four biomarkers were evaluated: Acetylcholinesterase (AChE), Glutathione S-transferase (GST), Catalase (Cat) and malondialdehyde activity (MDA). In parallel, physico-chemical parameters were measured in the marine water of Anza-Taghazout within three selected sites: S1 considered as ‘hotspot’ located at Anza city; S2 located near of Aourir city and the third site, S3 ‘reference’ located in Imouran beach. Our results showed that activities of both Gluthation-S-Transferase and Catalase were higher in M. galloprovincialis collected from site S1, but high values of Malondialdehyde and Acetylcholinesterase activities were observed successively at S3 and S2. Application of Integrated Biomarker Response index (IBR) was suitable for classifying the stress response in the M. galloprovincialis but did not allow to evaluate the level of the xenobiotic exposure in the studied sites. The statistical results did not show any significant differences between the three studied sites and therefore S1 has recently become clean due to the installation of two wastewater treatment plants.

Complex trauma is a clinical concept developed to address the nature and effects of interpersonal traumatic experiences over the development of children and adolescents. To contribute to the development of ecologically valid diagnostic tools and a better understanding of the effects of complex trauma, an ethogram of emotional regulation and stress response behaviours was proposed and used as the main instrument in the Crossed-Puzzles Game. In total 84 children and adolescents aged 6–14 (50% of children with complex trauma in state protective care) were selected from institutions and the community. Both groups were matched by age, sex, and socioeconomic status. PCA, PERMANOVA, Mann-Whitney’s U, Pearson correlations, and logistic regression analyses were performed. The ethogram’s category ‘Externalising of frustration’ was identified as one of the strongest predictors for the complex trauma group. The ethogram’s validity as a research and assessment instrument from a clinical ethology perspective of complex trauma in psychology was discussed.

Environmental history (regimes of water quality to which an organism has been exposed in the past) may influence how the physiology of eastern oysters Crassostrea virginica responds to future environmental conditions caused by climate change. Previous research has examined environmental history in a 1-dimensional framework, failing to capture environmental history complexity through space and time. In this study, we examined environmental history as a multi-faceted parameter, incorporating abiotic water quality components, such as temperature, pH, and salinity, that differ among locations. We also assessed how different lengths of environmental histories, defined as proximal and distal, affected oyster physiology and stress response. Finally, we compared the relative influence of abiotic components of environmental history on oyster physiology. We found that physiology and stress response are differentially affected by proximal and distal environmental history, demonstrating the importance of examining environmental history as a multi-faceted and dynamic parameter. Specifically, distal environmental history primarily influenced condition index and total antioxidant potential, while proximal environmental history primarily influenced glycogen content. Salinity of distal environmental history significantly shaped condition index, establishing salinity as a principal factor when considering acclimatization to variable environments. No water quality components were significant influences on glycogen and total antioxidant potential, providing opportunities for research on other components of environmental history. Identifying the temporal portion of oysters’ environmental history that influences physiology supports future efforts to predict population tolerance to climate change. Additionally, examining multiple abiotic and biotic components of environmental history can elucidate means of acclimatization to future environmental change.

1. This study investigated the influence of dietary supplementation of Solanum aethiopicum and Solanecio biafrae leaves, which have nutraceutical properties, on stress response, cytokine expression...

International audience; The present study aims at investigating the low cycle fatigue behavior of two fully AISI 1080 pearlitic steels which differed intheir interlamellar spacing (72 ± 15 and 143 ± 32 nm). Low cycle fatigue tests were performed under positive strain control atdifferent total strain variations (0.6% ≤ Δεt ≤ 1.6%). It is shown that both alloys presented an asymmetric stress response anda cyclic softening during the first fatigue life fractions. The tension stress peaks were higher for the fine pearlitic steel thanfor the coarse one but the compression stress peaks were less different. The extrusion–intrusion pairs formed at the externalsurface were more developed for the coarse pearlitic steel. The results suggest that the critical short crack size to propagatein the bulk is much smaller in the fine pearlitic steel which explains the shorter fatigue lives. For both steels, the entire fracturesurface comprised a fatigue propagation zone, a final fully brittle zone separated by a narrow ductile transition zone.

The authors aimed to investigate the effects of a laughter therapy (LT) program on the acculturative stress, anxiety, depression, salivary cortisol, and salivary IgA of married immigrant women in South Korea. A randomized waiting-list control design was utilized. The 19 experimental group participants were given LT twice a week for 2 weeks, whereas the 22 participants in the waiting-list control group received no treatment during the same period but received it afterward. Outcomes were measured right after the completion of the intervention and 2 weeks later. The experimental group showed significantly decreased levels of acculturative stress, anxiety, depression, and salivary cortisol compared to the control group. As for salivary IgA, however, the experimental group showed no statistically significant decrease compared to the control group. An LT program can be a good option for married immigrant women undergoing acculturation experiences in consideration of its effectiveness.

Objectives. We investigated the biological response of organism to stress in real situations at school in adolescents. We were interested in how stress is manifested in adolescents without anxiety and in adolescents with a tendency to anxiety experiencing. The aim of the study is to analyse changes in salivary cortisol levels during stressful situation at school. Hypotheses. The analyses verified how cortisol levels change in situations associated with the threat of social assessment in the school environment in individuals without anxiety and anxious; H1a–c: Anxiety individuals will have higher cortisol levels in all monitored situations compared to adolescentswithout anxiety. We also focused on the possible links between an individual’s tendency to experience anxiety and the type of stress response; H2: Anxiety individuals will show a different development of cortisol levels between situations A, B and C compared to adolescents without anxiety. We also examined the relationships between cortisol levels and cognitive and emotional fear. H3: Anxiety individuals will show significant links between cortisol levels and cognitive and emotional fear in the achievement situation. Methods. The research group was comprised of 238 adolescents in the first phase of the study. The final sample for salivary cortisol analysis was comprised of 38 participants aged 12−14 years. The questionnaires battery contains Piers-Harris II, B-JEPI and TAI. Results. The results show that in both groups of adolescents without anxiety and anxious adolescents, cortisol has a demonstrably different development trend during the day. Furthermore, anxious adolescents showed demonstrably lower cortisol levels at school compared to adolescents without anxiety, in adolescents without anxiety, we observed higher cortisol levels and fall of cortisol level during the day. Our analyses showed that stronger relationships between cortisol and test anxiety are shown especially by anxiety adolescents compared to adolescents without anxiety. Limitations. The limitation is a smaller research sample and the selection of situations in which the level of salivary cortisol was evaluated. The important limitation is the fact that the first sampling was taken at different times after awakening, because the cortisol levels change more dynamically in this part of the day.

The vertebrate glucocorticoid stress response is an important mechanism facilitating pleiotropic phenotypic adjustments for coping with environmental change and optimizing fitness. Although circulating glucocorticoid hormones are mediators of plasticity that individuals can adjust rapidly in response to environmental challenges, they are also shaped by ecological selection. It remains unclear, however, how environmental variation on different timescales influences glucocorticoids. Here, we use an intraspecific comparative approach to determine how variation in precipitation on different timescales (months, years, decades) shapes distinct components of the glucocorticoid response. We sampled superb starlings (

Diagnosing aging for preventative intervention generally relies on the tracking of aging biomarkers in the resting state. However, the static marker levels are insufficient to fully evaluate aging, particularly given that the stress response capacity (SRC) decay is currently viewed as a critical feature of aging. Therefore, we have developed a dual-channel fluorescent probe ROKS capable of the logic-based visualization of thiophenol (stressor) and HOCl (thiophenol-activated stress response product) in vivo, which provides a new strategy from the time dimension to precisely assess the SRC of individuals under stress using the dual-channel fluorescence ratio. Using ROKS we observed that the SRC of live cells decayed with senescence, and that a higher SRC was found for young vs. aged Caenorhabditis elegans. As such, our study offers a promising strategy for the fluorescence-guided diagnosis of aging and paves the way for accurate evaluation of the efficacy of anti-aging drugs., Rather than tracking aging using the resting state, ROKS, an optical probe, was developed for evaluating the degree of aging dynamically by precisely monitoring the stress response of individuals under stress.