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The increasing global food demand is threatening the sustainability of agrifood production systems. The intensification of agricultural practices, with inadequate use of pesticides and fertilizers, poses major challenges to the good functioning of agroecosystems and drastically degrades the soil quality. Nanotechnology is expected to optimize the current farming practices and mitigate some associated impacts. Layered double hydroxides (LDHs) are a class of nanomaterials with high potential for use in agricultural productions, mostly due to their sustained release of nutrients. Considering its novelty and lack of studies on the terrestrial ecosystem, it is essential to assess potential long-term harmful consequences to non-target organisms. Our study aimed to evaluate the effect of Zn-Al-NO 3 LDH and Mg-Al-NO 3 LDH ageing on the survival and reproduction of two soil invertebrate species Enchytraeus crypticus and Folsomia candida. We postulated that the toxicity of nanomaterials to soil invertebrates would change with time, such that the ageing of soil amendments would mediate their impacts on both species. Our results showed that the toxicity of LDHs was species-dependent, with Zn-Al-NO 3 LDH being more toxic to E. crypticus, while Mg-Al-NO 3 LDH affected more F. candida, especially in the last ageing period, where reproduction was the most sensitive biological parameter. The toxicity of both nanomaterials increased with ageing time, as shown by the decrease of the EC 50 values over time. The influence of LDH dissolution and availability of Zn and Mg in the soil pore water was the main factor related to the toxicity, although we cannot rule out the influence of other structural constituents of LDHs (e.g., nitrates and aluminium). This study supports the importance of incorporating ageing in the ecotoxicity testing of nanomaterials, considering their slow release, as effects on soil organisms can change and lead to more severe impacts on the ecosystem functioning., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Nanoagrochemicals have the potential to revolutionize agriculture towards a precision farming system, able to reduce application rates and consequently their environmental footprint, while keeping efficacy. Several nanoagrochemicals (including nanopesticides (Npes) and nanofertilizers (Nfer)) are already commercialized but the environmental risk assessment of these advanced materials is often lacking. In the present study, we studied the commercial fertilizer WELGRO® Cu + Zn and assessed its ecotoxicity to the soil invertebrate species Enchytraeus crypticus (Oligochaeta), further comparing it to its individual active substances CuO and ZnO. To get a comprehensive picture of possible effects, we used four types of highly relevant tests in LUFA 2.2 soil: 1) avoidance behaviour (2 days), 2) reproduction (OECD standard, 28 d), 3) its extension (56 d), and 4) the full life cycle (FLC) (46 d) - this high level of hazard screening allows for increased interpretation. The results confirmed the nano-features of WELGRO® and a higher toxicity than the mixture of the individual components CuO + ZnO. E. crypticus avoided the soil spiked with WELGRO® and CuO + ZnO, this being the most sensitive endpoint - avoidance behaviour. Both WELGRO® and the active substances were little to non-toxic based on the OECD standard test. However, the toxicity dramatically increased in the tests focussing on longer-term sustainability measures, i.e., 56 days, ca. 170 for WELGRO®. This seems related to the nano-features of WELGRO®, e.g., slow release of ions from the nanoparticles throughout time. The FLCt results showed WELGRO® affected hatching and juveniles' survival, being these the most sensitive life stages. Hence, under actual real world field usage scenarios, i.e., based on the recommended application rates, nanoenabled WELGRO® can affect oligochaete populations like enchytraeids, both via the immediate avoidance behaviour and also via prolonged exposure periods., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Terrestrial snails are a significant issue in agricultural production worldwide. The use of nitrogen - phosphorus - potassium (NPK) based fertilizers played an important role in meeting the food demand throughout the world, so its effectiveness against land snails needs to be investigated. This study was conducted to evaluate toxic lethal effect of New-Fort®, an inorganic NPK based fertilizer, in the field for 3, 7 and 10 days and in the laboratory for 24, 48 and 72 h against Theba pisana snails. Also, the impact of its sub-lethal doses (1/10, 1/5, 1/4 and 1/2 of 48 h-LD 50 ) on biochemical parameters were determined under laboratory conditions. The results showed that the snails percent reduction in the field were 21.4, 61.0 and 80.0 % after 10 days' application of quarter, half and one field rate and the values of LD 50 in the laboratory were 4.94, 4.56 and 4.24 mg/g b.w at 24, 48 and 72 h, respectively. New-Fort® sub-lethal doses caused a significant inhibition in catalase, γ-glutamyl transferase and acetylcholinesterase activities. It also elicited a significant elevation in glutathione S-transferase activity post exposure to 1/10 and 1/5 of LD 50 , whereas an opposite effect was occurred after exposure to 1/4 and 1/2 of LD 50 . Lipid peroxidation level was reduced in snails treated with 1/10 and 1/5 of LD 50 , whereas it increased in 1/4 and 1/2 of LD 50 - treated snails. Moreover, a significant inhibition in alkaline phosphatase activity at all tested doses, with the exception of 1/2 of LD 50 was observed. An increase in alanine aminotransferase and aspartate aminotransferase activities were occurred after all tested doses exposure. Our findings highlighted on how biochemical changes can be exploited to better understand the mechanisms underlying New-Fort® fertilizer toxicity against the land snail, T. pisana, as well as how to benefit from NPK fertilizers application in snail control., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Soil microplastic pollution can have negative effects on organisms, including plants, but the underlying mechanisms are not fully understood. We tested whether structural or chemical properties of a microplastic cause its effects on plant above- and belowground growth and whether these effects can be influenced by earthworms. We conducted a factorial experiment in a greenhouse with seven common Central European grassland species. Microplastic granules of the synthetic rubber ethylene propylene diene monomer (EPDM), 1 a frequently used infill material of artificial turfs, and cork granules with a comparable size and shape to the EPDM granules were used to test for structural effects of granules in general. To test for chemical effects, EPDM-infused fertilizer was used, which should have contained any leached water-soluble chemical components of EPDM. Two Lumbricus terrestris individuals were added to half of the pots, to test whether these earthworms modify effects of EPDM on plant growth. EPDM granules had a clear negative effect on plant growth, but since cork granules had a negative effect of similar magnitude, with an average decrease in biomass of 37 % in presence of granules, this is likely due to the structural properties of granules (i.e., size and shape). For some belowground plant traits, EPDM had a stronger effect than cork, which shows that there must be other factors playing into the effects of EPDM on plant growth. The EPDM-infused fertilizer did not have any significant effect on plant growth by itself, but it had in interaction with other treatments. Earthworms had an overall positive effect on plant growth and mitigated most of the negative effects of EPDM. Our study shows that EPDM microplastic can have negative effects on plant growth, and that these might be more related to its structural than to its chemical properties., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

A multitude of possible contaminants can be contained in bio-based fertilizers (BBFs) because of their complex matrix. The chemical characterization of BBFs is a challenging analytical task. Therefore, it is important for sustainable agricultural production to develop standard procedures to assess new bio-based fertilizers for possible hazards related to their application in order to guarantee their safety for soils organisms, plants and the environment. There is a huge number of ecotoxicological tests for aquatic and terrestrial organisms. They were developed for the evaluation of chemicals, pesticides and industrial wastes on aquatic systems and soil functioning. These tests can be useful for the assessment of BBFs. Ecotoxicological tests in comparison to chemical analysis have the advantage to capture the effects of all possible contaminants and metabolites available in the product. The bioavailability of toxic compounds and their interaction are recorded while the cause-and-effect-chain is not elucidated. Numerous ecotoxicological tests work with liquid media, capturing the effects of pollutants that can be mobilized. Hence, standardized procedures how to produce solvents from BBFs are mandatory. Moreover, tests using the original (solid) material are necessary in order to determine the toxicity of a given BBF in its application form and to cover the potential toxicity of non-soluble compounds. To date there are no rules how to determine the ecotoxicological potential of BBFs. A tiered approach of chemical analytical parameters in combination with a set of ecotoxicological tests and the measurement of sensitive soil indicators seem to be a promising experimental setup for the evaluation of BBFs. A decision tree for such an approach was developed. An extended ecotoxicological test strategy of BBFs is mandatory to identify the most promising raw materials and BBF processing technologies to end up with sustainable fertilizer products showing a high agronomic efficiency., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

The demand for food to feed the growing world population has been promoting the indiscriminate use of chemical fertilizers, which can be detrimental to the environment. In order to maintain high crop productivity without damaging the ecosystem, biofertilizers have emerged as alternative to reduce the use of chemical fertilizers. So, environmentally safer biofertilizer can replace the exploitation of more toxic chemical fertilizer. Here, the fly Drosophila melanogaster was used to study the potential toxicity of the biofertilizer Beifort®. Flies were exposed to high concentrations of Beifort® in the diet (1.8 mL/L, 9.0 mL/L and 18 mL/L), and morphological and behavioral endpoints of toxicity were analyzed (development from egg to adult age, flies longevity, climbing performance, memory and learning of an associative learning, larvae digestive tract damage and plasmid DNA break). Beifort® did not modify flies development, survival, digestive track cell damage, locomotor activity or memory. Beifort® did not induce DNA breakage in vitro and had no toxicity to the non-target D. melanogaster after in vivo exposure. Thus, in addition of promoting the sustainable use of agricultural wastes, the exploitation of Beifort® can contribute to decrease the use of chemical fertilizers., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

The huge volumes of currently generated agricultural waste pose a challenge to the economy of the 21st century. One of the directions for their reuse may be as fertilizer. Spent mushroom substrate (SMS) could become an alternative to manure (M). A three-year field experiment was carried out, in which the purpose was to test and compare the effect of SMS alone, as well as in multiple variants with mineral fertilization, and in manure with a variety of soil quality indices-such as enzymatic activity, soil phytotoxicity, and greenhouse gas emissions, i.e., CO 2 . The use of SMS resulted in significant stimulation of respiratory and dehydrogenase activity. Inhibition of acid phosphatase and arylsulfatase activity via SMS was recorded. SMS showed varying effects on soil phytotoxicity, dependent on time. A positive effect was noted for the growth index (GI), while inhibition of root growth was observed in the first two years of the experiment. The effect of M on soil respiratory and dehydrogenase activity was significantly weaker compared to SMS. Therefore, M is a safer fertilizer as it does not cause a significant persistent increase in CO 2 emissions. Changes in the phytotoxicity parameters of the soil fertilized with manure, however, showed a similar trend as in the soil fertilized with SMS.

Stress exposures during vegetation are known to reduce the yield in crops, but the intensity and duration of stress is rather difficult to determine from the crop loss. Allelotoxins are released from plants into soil on exposure to stress factors. Soil allelotoxicity after vegetation was assumed to provide a diagnostic parameter that characterizes the total stress effect on crops. To verify the assumption, changes in soil allelotoxicity were studied after vegetation of three winter wheat cultivars fed with different amounts of nitrogen fertilizers. The cultivars Nemchinovskaya 85, Moskovskaya 39, and Lutescens 982/08 were found to differently respond to fertilizing. Nemchinovskaya 85 and Lutescens 982/08 showed lower stress responses at higher doses of nitrogen fertilizers, while their responses were appreciable when soil nitrogen was lacking. In the case of Moskovskaya 39, higher doses of nitrogen fertilizers caused an adverse reaction of increased allelotoxin release. Stepwise nitrogen fertilization was concluded to be necessary for preventing delayed plant development in the case of this cultivar. The results confirmed that soil allelotoxicity measured growing plants can serve as an indicator of stress caused by the growth conditions and use of various agricultural techniques., (© 2022. Pleiades Publishing, Ltd.)

Whether maternal macro-environmental chemical fertilizer exposure has an association with the risk of preterm birth remains unclear. This study aimed to investigate the association between maternal exposure to chemical fertilizer during pregnancy and the risk of preterm birth. A total of 13,861 births, including 402 preterm birth from 313 villages in Pingding County, Shanxi Province, China, were analyzed to explore spatial variations of preterm birth risks at the village level. Spatial variations of preterm birth were visualized and tested with Disease Mapping, Moran's I and G* Statistic. The spatial zero-inflated negative binomial model was used to evaluate the incidence rate ratio (IRR) of preterm birth in different chemical fertilizer consumption levels at the village level. A population-based case-control study was conducted including 153 preterm births cases and 204 controls at the household level. The two-level logistic regression model was performed to estimate the association between risks of preterm birth and maternal exposure to chemical fertilizer after adjusting confounding factors. The findings indicated a remarkably clustering effect in relative risks of preterm birth and identified a high-risk clustering region of preterm birth from the south-central to the southwest and a low-risk clustering region in the northern Pingding county. The results of the spatial zero-inflated negative binomial model showed that the risk of preterm birth in the villages with chemical fertilizer consumption≥100 tons was 2.82 (95% CI: 1.50-5.57) times higher than those with <50 tons. Maternal exposure to chemical fertilizer ≥100 tons at village level was associated with an increased risk of preterm birth (aOR 2.14, 95%CI: 1.18-3.96), compared with exposure <50 tons after adjusting for confounding variables. The findings suggests that chemical fertilizers deserve more attention as a potential risk factor of preterm birth., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The increasing use of agrochemicals, alone and in combination, has been implicated as a potential causative factor in the decline of amphibians worldwide. Fertilizers and pesticides are frequently combined into single-use tank mixtures for agricultural applications to decrease costs while meeting the food demands of a growing human population. Limited data are available on the effects of increased nitrogen levels in nontarget species, such as amphibians, and therefore investigating alterations in the nitrogen cycle and its impacts on amphibians needs to be considered in best management practices going forward. The objective of the present study was to elucidate the impact of fertilizer (urea) and herbicide (atrazine and/or alachlor) tank mixtures on the hepatic metabolome of juvenile leopard frogs as well as to investigate alterations in oxidative stress by relating these changes to glutathione (GSH) levels. Herbicide exposure only moderately increased this parameter in amphibians, however, urea alone and in combination with either atrazine or alachlor statistically elevated GSH levels. Interestingly, urea also inhibited pesticide uptake: calculated bioconcentration factors were greatly decreased for atrazine and alachlor when urea was present in the exposure mixture. Metabolomic profiling identified fluxes in hepatic metabolites that are involved in GSH and carbohydrate metabolic processes as well as altered intermediates in the urea cycle. Ultimately, understanding the biological impacts of nitrogenous fertilizers alone and in combination with pesticide exposure will inform best management practices to conserve declining amphibian populations worldwide. Environ Toxicol Chem 2022;41:122-133. © 2021 SETAC., (© 2021 SETAC.)

Exposure to anthropogenic environmental chemical mixtures could be contributing to the decline in male reproductive health. This study used the biosolid treated pasture (BTP) sheep model to assess the effects of exposure to low-dose chemical mixtures. Maternal BTP exposure was associated with lower plasma testosterone concentrations, a greater proportion of Sertoli cell-only seminiferous tubules, and fewer gonocytes in the testes of neonatal offspring. Transcriptome analysis highlighted changes in testicular mTOR signalling, including lower expression of two mTOR complex components. Transcriptomic hierarchical analysis relative to the phenotypic severity demonstrated distinct differential responses to maternal BTP exposure during pregnancy. Transcriptome analysis between phenotypically normal and abnormal BTP lambs demonstrated separate responses within the cAMP and PI3K signalling pathways towards CREB. Together, the results provide a potential mechanistic explanation for adverse effects. Exposure could lower gonocyte numbers through mTOR mediated autophagy, but CREB mediated survival factors may act to increase germ cell survival., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Juvenile African Catfish (also known as Sharptooth Catfish) Clarias gariepinus were exposed to 2.26, 4.52, and 11.30 mg/L NPK (15-15-15) fertilizer for 21 d followed by 7 d of recovery to assess the genotoxic effects of the fertilizer in erythrocytes. Biomarkers of oxidative stress were evaluated in the liver and gill tissues. The fertilizer induced micronuclei formation with maximum effects on day 7 in erythrocytes of individuals that were exposed to 4.52 and 11.30 mg/L NPK, and on day 14 in individuals exposed to 2.26 mg/L of the same fertilizer. The lipid peroxidation, glutathione reductase, and reduced glutathione values in the exposed fish increased, while the values of catalase, superoxide dismutase, and glutathione peroxidase decreased. There were mixed trends in the recovery patterns after the 7-d withdrawal from the fertilizer. Careful use of the fertilizer in the field is recommended to avoid toxicological effects on nontarget organisms., (© American Fisheries Society 2020.)

Poultry litter is used as a fertilizer due to the high concentration of nutrients. However, this material receives application of medicines, whether they are used to treat animals or the litter itself. Thus, the objective was to evaluate if the application in soils of poultry litter doses treated with two insecticides (cypermethrin and cinnamon oil) used to control of the Alphitobius diaperinus, affect the reproduction of Enchytraeus crypticus, and the insecticides effects over time, trough of ecotoxicological tests using soils with different textural classes an Entisol and an Oxisol. In the Oxisol the application of poultry litter stimulated the E. crypticus reproduction regardless of the insecticide and the collection time. In Entisol the poultry litter showed a toxicity at 10 t ha -1 when treated with cypermethrin and at 30 t ha -1 with cinnamon oil. Entisol results show that cypermethrin affects non-target edaphic organisms soon after spraying, but over time has reduced toxicity unlike cinnamon oil., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020. Published by Elsevier B.V.)

The QuEChERS (quick, easy, cheap, effective, rugged, and safe) ultrasound-assisted method was successfully used to determine the presence of contaminants of emerging concern (CECs) in both the growing medium (i.e. soil) and vegetable samples (i.e. potato, onion, celery, parsnip and carrot) cultivated in household gardens under field conditions impacted by the application of livestock manure mixed with human waste. CECs with a broad range of physico-chemical properties including pharmaceuticals, plasticizers, herbicides, personal care products, and biocides were investigated. Among all studied CECs, diclofenac (DCL), carbamazepine, bisphenol A (BPA) and estrogenic hormones (estrone (E1), 17α-ethinyl estradiol (EE2)) were detected and quantified in vegetable samples with a concentration range from 0.114 to 13.3 ng/g fresh weight (f.w.), while BPA, E1, and EE2 were detected in soil samples with concentration ranges from 0.526 to 0.830 ng/g f.w., 0.121-0.199 ng/g f.w. and 0.118-0.333 ng/g f.w., respectively. DCL was only quantified above the limit of quantification in one soil sample (0.151 ng/g f.w.). The human health risk was estimated using the threshold of toxicological concern approach and a standard diet for two segments of the population (i.e. adult and child). The consumption of investigated vegetables contaminated with CECs poses no risk for the Serbian population., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Purpose: The aim of this study was to prepare zeolite/iron (III) oxide nanocomposites (zeolite/Fe 2 O 3 -NCs) as a smart fertilizer to improve crop yield and soil productivity., Methods: Zeolite/Fe 2 O 3 -NCs were successfully produced by loading of Fe 2 O 3 -NPs onto the zeolite surface using a quick green precipitation method. The production of zeolite/Fe 2 O 3 nanocomposites was performed under a mild condition using environmentally friendly raw materials as a new green chemistry method. The product was characterized using several techniques such as near and far Fourier-transform infrared spectroscopy (FT-IR), powder X-ray diffraction (PXRD), energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM) and transmission electron microscopy (TEM)., Results: The results confirmed the formation of Fe 2 O 3 -NPs with mean particle sizes of 1.45, 2.19, and 2.20 nm on the surface of the zeolite per amount of 4, 7 and 12 wt% Fe 2 O 3 -NPs, respectively. Such results indicated that the size of the Fe 2 O 3 -NPs did not significantly change when Fe amounts increased from 7 to 12 wt% for the zeolite/Fe 2 O 3 -NCs. In terms of medical applications, in vitro cell studies demonstrated that zeolites and zeolite/Fe 2 O 3 -NCs were generally non-toxic to human fibroblast cells and significantly pernicious to human malignant melanoma cells. From MTS cytotoxicity assays, the concentration of Fe 2 O 3 within the zeolite/Fe 2 O 3 -NCs that was effective at inhibiting the growth of malignant melanoma cells by 50% (the IC50 value) was ~14.9 wt%. The three types of nanocomposites were further tested as an iron smart nanofertilizer for the slow-release of iron ions., Conclusion: Advantages of this project include the production of non-toxic nanocomposites as a smart fertilizer to develop crops while the reaction involves the use of commercial and natural materials as low-cost raw materials with low energy usage due to a mild reaction condition, as well as the use of an environmentally friendly solvent (water) with no toxic residues., Competing Interests: The authors declare that they have no conflicts of interest in this work., (© 2020 Jahangirian et al.)

Increasing agricultural production requires the application of more chemical inputs during the growth of different crops. This study aims at assessing the energy indicators and environmental impacts during the initial 7-year growth of orange orchards. Through the life cycle assessment (LCA) technique, the hotspots in 11 environmental indicators of the orange orchard growth were determined for different years. The system boundaries were considered to be the orange orchard gates, and the functional units were 1 kg of orange and 1-h orange orchard. The energy input of nitrogen fertilizer, diesel, and human labor has the biggest share in the total energy inputs. The total average energy input and energy output (in 7 years) were calculated as 62,917.027 MJ/ha and 47,618.17 MJ/ha, respectively. The results showed that the energy indices (energy efficiency and net energy) were increasing from year first to seventh and also the share of renewable energy increased. According to the results, with respect to the highest share for production of 1 kg orange in different criteria, nitrogen fertilizer was the main contributor to abiotic depletion and human toxicity, orchard field emissions and nitrogen fertilizer had the highest shares in global warming and photochemical oxidation, fossil fuels and nitrogen fertilizer were the highest contributors to ozone layer depletion, whereas ecotoxicity was mainly affected by chemical fertilizers and orchard surface emissions. Finally, the main contributor for acidification and eutrophication was surface emissions. In the study of environmental impacts from the first to the seventh year, it should be noted that if the functional unit is considered mass based (1 kg orange), due to the unproductive of the trees in the first to third years (low fruit production), the environmental effects are high and then it decreases after the third year due to increasing the yield of the product. Generally, with consideration of the tree growth period, the useful data of energy and environmental impacts for production horticultural products can be provided so that we can avoid multiple interpretations of results associated to reporting annual energy and environmental impact variations.

The rapid development and exploitation of the Yangtze River basin in order to ensure human food security and increase living space in recent decades has resulted in significant potential for degradation of water quality in the river and in hundreds of lakes. Understanding how lake environments have evolved to their present state under a variety of external influences is crucial for evaluating their current status and anticipating future scenarios of environmental changes. However, the lakes along the middle reaches of the Yangtze River (MRY) are as yet little studied. Here, we described the long-term anthropogenic environmental transformations of a small lake (Lake Taibai) in the MRY area, based on a detailed quantitative geochemical analysis of the aliphatic hydrocarbons, nutrients (N and P), biogenic silica (BSi), and major and trace elements present in a dated sediment core retrieved from the lake. Our data revealed that levels of short-chain n-alkanes, αβ-hopanes and the trace elements arsenic (As) and cadmium (Cd) were all low for the entire record in sediments prior to ca. 1970, reflecting unpolluted natural state of the lake. Pronounced anthropogenic effects began to appear in sediments deposited in the subsequent years ca. 1970-1990, during which the levels of all these components were elevated, most likely driven by input of nitrogen (N) and phosphorus (P) containing chemical fertilizers, pesticides and diesel oil respectively. Since ca. 1990, changes of short-chain n-alkane levels in the sediment suggested the lake had undergone dramatic eutrophication in which existing anthropogenic stressors were exacerbated by technological advances that extended the use of chemical fertilizer into aquaculture. This pattern contrasted with an otherwise comparable lake in the lower Yangtze River basin, Lake Changdang, in which trace element and petroleum pollution were much more prominent due to dramatic urbanization and industrialization of the catchment., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The exposure of bees to agrochemicals during foraging and feeding has been associated with their population decline. Sublethal exposure to agrochemicals can affect behavior and the microbiota. Gut microbiota is associated with insect nutritional health, immunocompetence, and is essential for neutralizing the damage caused by pathogens and xenobiotics. Research on the effect of the bioinsecticides and fertilizers on the microbiota of bees remains neglected. In this study, we assessed the sublethal effect of both bioinsecticide spinosad and the fertilizer copper sulfate (CuSO 4 ) on the behavior and gut microbiota in forager adults of the stingless bee Partamona helleri (Friese), which is an important pollinator in the Neotropical region. Behavioral assays and gut microbiota profiles were assessed on bees orally exposed to estimated LC 5 values for spinosad and CuSO 4 . The microbiota were characterized through 16S rRNA gene target sequencing. Acute and oral sublethal exposure to spinosad and CuSO 4 did not affect the overall activity, flight take-off, and food consumption. However, CuSO 4 decreased bee respiration rate and copper accumulated in exposed bees. Exposure to spinosad increased the proportional abundance of the genus Gilliamella, but CuSO 4 did not alter the composition of the gut microbiota. In conclusion, sublethal exposure to CuSO 4 induces changes in respiration, and spinosad changes the abundance of gut microorganisms of P. helleri., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Background: N fixation is one of the most important microbially driven ecosystem processes on Earth, allowing N to enter the soil from the atmosphere, and regulating plant productivity. A question that remains to be answered is whether such a fundamental process would still be that important in an over-fertilized world, as the long-term effects of fertilization on N fixation and associated diazotrophic communities remain to be tested. Here, we used a 35-year fertilization experiment, and investigated the changes in N fixation rates and the diazotrophic community in response to long-term inorganic and organic fertilization., Results: It was found that N fixation was drastically reduced (dropped by 50%) after almost four decades of fertilization. Our results further indicated that functionality losses were associated with reductions in the relative abundance of keystone and phylogenetically clustered N fixers such as Geobacter spp., Conclusions: Our work suggests that long-term fertilization might have selected against N fixation and specific groups of N fixers. Our study provides solid evidence that N fixation and certain groups of diazotrophic taxa will be largely suppressed in a more and more fertilized world, with implications for soil biodiversity and ecosystem functions.

The adaptation of crops to acid soils is needed for the maintenance of food security in a sustainable way, as decreasing fertilizers use and mechanical interventions in the soil would favor the reduction of agricultural practices' environmental impact. Phosphate deficiency and the presence of reactive aluminum affect vital processes to the plant in this soil, mostly water and nutrient absorption. From this, the understanding of the molecular response to these stresses can foster strategies for genetic improvement, so the aim was to broadly analyze the transcriptional variations in Poupulus spp. in response to these abiotic stresses, as a plant model for woody crops. A co-expression network was constructed among 3,180 genes differentially expressed in aluminum-stressed plants with 34,988 connections. Of this total, 344 genes presented two-fold transcriptional variation and the group of genes associated with those regulated after 246 hours of stress had higher number of connections per gene, with some already characterized genes related to this stress as main hubs. Another co-expression network was made up of 8,380 connections between 550 genes regulated by aluminum stress and phosphate deficiency, in which 380 genes had similar profile in both stresses and only eight with transcriptional variation higher than 20%. All the transcriptomic data are presented here with functional enrichment and homology comparisons with already characterized genes in another species that are related to the explored stresses, in order to provide a broad analysis of the co-opted responses for both the stresses as well as some specificity. This approach improves our understanding regarding the plants adaptation to acid soils and may contribute to strategies of crop genetic improvement for this condition that is widely present in regions of high agricultural activity., Competing Interests: The authors have declared that no competing interests exist.

Chicken litter application on land as an organic fertilizer is the cheapest and most environmentally safe method of disposing of the volume generated from the rapidly expanding poultry industry worldwide. However, little is known about the safety of chicken litter for land application and general release into the environment. Bridging this knowledge gap is crucial for maximizing the benefits of chicken litter as an organic fertilizer and mitigating negative impacts on human and environmental health. The key safety concerns of chicken litter are its contamination with pathogens, including bacteria, fungi, helminthes, parasitic protozoa, and viruses; antibiotics and antibiotic-resistant genes; growth hormones such as egg and meat boosters; heavy metals; and pesticides. Despite the paucity of literature about chicken litter safety for land application, the existing information was scattered and disjointed in various sources, thus making them not easily accessible and difficult to interpret. We consolidated scattered pieces of information about known contaminants found in chicken litter that are of potential risk to human, animal, and environmental health and how they are spread. This review tested the hypothesis that in its current form, chicken litter does not meet the minimum standards for application as organic fertilizer. The review entails a meta-analysis of technical reports, conference proceedings, peer-reviewed journal articles, and internet texts. Our findings indicate that direct land application of chicken litter could be harming animal, human, and environmental health. For example, counts of pathogenic strains of Eschericia coli (10 5- 10 10 CFU g -1 ) and Coliform bacteria (10 6 -10 8 CFU g -1 ) exceeded the maximum permissible limits (MPLs) for land application. In Australia, 100% of broiler litter tested was contaminated with Actinobacillus and re-used broiler litter was more contaminated with Salmonella than non-re-used broiler litter . Similarly, in the US, all (100%) broiler litter was contaminated with Eschericia coli containing genes resistant to over seven antibiotics, particularly amoxicillin, ceftiofur, tetracycline, and sulfonamide. Chicken litter is also contaminated with a vast array of antibiotics and heavy metals. There are no standards set specifically for chicken litter for most of its known contaminants. Even where standards exist for related products such as compost, there is wide variation across countries and bodies mandated to set standards for safe disposal of organic wastes. More rigorous studies are needed to ascertain the level of contamination in chicken litter from both broilers and layers, especially in developing countries where there is hardly any data; set standards for all the contaminants; and standardize these standards across all agencies, for safe disposal of chicken litter on land., Competing Interests: There is no conflict of interest among the authors.

Phthalate acid esters (PAEs) are the most frequently utilized synthetic chemical compounds worldwide. They are typical emergent contaminants and are currently attracting considerable concern due to their risks to plants, animals, and public health. Determining the vital environmental factors that affect the toxicity of target pollutants in soil is important for vegetable production and the maintenance and control of soil productivity. We investigated the influence of di- n -butyl phthalate (DBP) and bis(2-ethylhexyl) phthalate (DEHP) under different soil conditions on physiological changes in water spinach ( Ipomoea aquatic Forsk.) seedlings and the rhizosphere soil microbial community. Supported by our former experiments in which we determined the representative concentrations that caused the most pronounced toxic effects, three experimental concentrations were studied including control soils without PAEs and spiked soils with either 20 mg DBP or DEHP kg -1 soil. The soil at all the three PAE concentrations was then adjusted to test two soil pH values, three levels of soil organic matter (SOM) content, and three levels of soil moisture content; thus, we completed 12 treatments or conditions simulating different soil environment conditions in greenhouses. After 30 days of cultivation, we analyzed the toxicity effects of two target PAEs on plant growth and physiological factors, and on soil microbial community characteristics. The toxicity of soil DBP and DEHP to the physiology of water spinach was found to be most affected by the soil pH value, then by SOM content, and least of all by soil moisture. The results of the 454 high-throughput sequencing analysis of the soil microbial community indicated that the toxicity of target PAEs to soil microorganisms was most affected by SOM content and then by soil moisture, and no clear relationship was found with soil pH. Under different soil conditions, declines in leaf biomass, chlorophyll a content, and carotenoid content-as well as increases in free amino acid (FAA) content, superoxide anion free radical activity, and hydroxyl radical activity-occurred in response to DBP or DEHP. Heavy use of chemical fertilizer, organic fertilizer, and high humidity led to the special environmental conditions of greenhouse soil, constituting the main conditions considered in this study. The results indicate that under the special highly intensive production systems of greenhouses, soil conditions may directly influence the effects of pollutant phytotoxicity and may thus endanger the yield, nutrient content, and food safety of vegetables. The combined studies of the impacts on plants and rhizosphere microorganisms give a more detailed picture of the toxic effects of the pollutants under different soil conditions.

Soybean farmers are exposed to various types of pesticides that contain in their formulations a combination of chemicals with genotoxic and mutagenic potential. Therefore, the objective of this paper was to evaluate the genetic damages caused by this pesticide exposure to soybean producers in the state of Mato Grosso (Brazil), regarding biochemical, genetic polymorphic and in silico analyses. A total of 148 individuals were evaluated, 76 of which were occupationally exposed and 72 were not exposed at all. The buccal micronucleus cytome assay (BMCyt) detected in the exposed group an increase on DNA damage and cell death. No inhibition of butyrylcholinesterase (BchE) was observed within the exposed group. The detection of inorganic elements was made through the particle-induced X-ray emission technique (PIXE), which revealed higher concentrations of Bromine (Br), Rubidium (Rb) and Lead (Pb) in rural workers. A molecular model using in silico analysis suggests how metal ions can cause both DNA damage and apoptosis in the exposed cells. Analysis of the compared effect of X-ray Repair Cross-complement Protein 1 (XRCC1) and Paraoxonase 1 (PON1) genotypes in the groups demonstrated an increase of binucleated cells (exposed group) and nuclear bud (non-exposed group) in individuals with the XRCC1 Trip/- and PON1 Arg/- genes. There was no significant difference in the telomere (TL) mean value in the exposed group in contrast to the non-exposed group. Our results showed that soybean producers showed genotoxic effect and cell death, which may have been induced by exposure to complex mixtures of agrochemicals and fertilizers. In addition, XRCC1 Arg/Arg could, in some respects, provide protection to individuals., (Copyright © 2019. Published by Elsevier Inc.)

Amending soils with biochar is increasingly proposed as a solution to many pressing agricultural and environmental challenges. Biochar, created by thermochemical conversion of biomass in an oxygen-limited environment, has several purported benefits, including remediation of contaminated soils, increased crop yields, reduced fertilizer demands, increased plant available water, and mitigation of climate change. Due to these potential benefits, biochar-related research has flourished in the past decade, though there remains a critically understudied area of research regarding biochar's potential impact on human health. Because biochar characteristically has low bulk density and high porosity, the material is susceptible to atmospheric release via natural or mechanical soil disturbance. The specific risks of biochar inhalation have not been elucidated; however, recent publications have demonstrated that biochar can increase soil dust emissions of particles <10 μm (PM 10 ) or possess elevated levels of toxic chemicals. These data should not be interpreted to suggest that all biochars are problematic, but rather to highlight an important and overlooked field of study, and to stress the need to critically assess parameters for biochar production and management strategies that safeguard human health. Here the literature on biochar-related dust emissions and potentially toxic properties (PTPs) is reviewed in order to summarize what is known, highlight areas for future study, and aggregate solutions to minimize potential harm., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Human activities have introduced a variety of chemicals, including pesticides, fertilizers, and salt, into the environment, which may have deleterious effects on the organisms inhabiting these areas. Amphibians are especially susceptible to absorption of chemical pollutants. To determine the possible combined effects of these chemicals on amphibian development and stress levels, Southern leopard frog (Lithobates sphenocephala) larvae were exposed to one of eight individual or combined treatments of atrazine, ammonium nitrate fertilizer, and sodium chloride salt. Stress levels, indicated by release of the stress hormone corticosterone, were measured premetamorphosis at week 8 of development. Water hormone samples were processed to analyze corticosterone levels. Changes in tadpole growth were determined by surface area measurements taken from biweekly photographs. The combined chemical treatment of atrazine, salt, and fertilizer had a significant interactive effect by increasing stress levels before metamorphosis (p = 0.003). After a month of larval development, tadpoles exposed to ammonium nitrate had larger surface area (p = 0.035). Tadpoles exposed to atrazine had a lower growth rate throughout larval development (p = 0.025) and the lowest number of individuals reaching metamorphosis at 33%. However, the frogs in the atrazine treatment that did successfully metamorphose did so in fewer days (p = 0.002). Because amphibians are exposed to multiple chemicals simultaneously in the environment, assessing the effects of a combination of contaminants is necessary to improve application strategies and ecosystem health.

Mustard oil cake (MOC) is widely used as biofertilizer in the field of agriculture and aquaculture. Channa punctatus was exposed to 0.42 g.L -1 sublethal concentration for 4, 7, 14, 21 and 28 days. Due to such exposure, body growth and histological changes in liver were observed. It was revealed that weight, length and breadth of fish were gradually increased with the days of exposure in compare to control fish, whereas, liver showed an increase in sinusoidal space and lipidosis during early days, followed by a recovery from the stress of MOC on the 28th day.

Tank mixtures are popular within the agricultural community because they are time- and cost-effective, but field applications leave nontarget organisms at risk of exposure. We explored the effects of a common herbicide (atrazine and alachlor) and fertilizer (urea) tank mixture on juvenile frog corticosterone stress levels, acetylcholinesterase (AChE) activity, and pesticide bioaccumulation. Single agrochemical or tank mixtures were applied to terrestrial microcosms, and then individual Southern leopard frog (Lithobates sphenocephala) juveniles were added to microcosms for an 8-h exposure. Afterward, frogs were transferred to aquatic microcosms for 1 h to monitor corticosterone prior to euthanasia, brain tissues were excised to evaluate AChE, and tissue homogenates were analyzed for pesticide bioconcentation with gas chromatography-mass spectrometry. Atrazine significantly increased corticosterone in frogs, particularly when combined with alachlor and urea. Atrazine increased AChE and urea decreased AChE, although no interactive effects of chemical combinations were discernible. Relative to their individual treatments, the complete tank mixture with all 3 agrochemicals resulted in 64% greater bioconcentration of atrazine and 54% greater bioconcentration of alachlor in frog tissues. Our results suggest that agrochemical mixtures as well as their active ingredients can lead to altered stress levels and impaired physiological responses in amphibians. An improved understanding of the effects of co-exposure to environmental contaminants in amphibians is important in assessing the ecological risks these compounds pose. Environ Toxicol Chem 2019;9999:1-10. © 2019 SETAC., (© 2019 SETAC.)

Cerium (Ce) is present in high technology materials and in mineral P fertilizers and the use and discharge of such resources may change the natural status of Ce in the soil environment. Brazilian soils in farming areas are significantly exposed to increased levels of unintentionally-added Ce through intensive input of phosphate fertilizers. The aims of this study were to evaluate the ecotoxicological risk to plants growing in tropical soils contaminated with Ce, as well as to create a database to support future legislation regulating the limits of this element in Brazilian and conceivably other tropical soils. Eight crop species (corn, sorghum, rice, wheat, soybeans, sunflower, radish, and beans) were exposed to a Ce concentration gradient in two typical tropical soils (Oxisol and Inceptsol), and an artificial soil. Our findings showed that among the endpoints measured, Ce phytotoxicity was more pronounced on shoot dry matter than on percent germination and germination speed index. Sensitivity of plants is species specific and our data showed that sunflower and radish exposed to Ce were the most sensitive crop species. Soil properties such as pH, cation exchange capacity, and organic carbon may have influenced the severity of Ce phytotoxicity. Because of that, the Oxisol contaminated with this element caused higher phytotoxicity than the other soils tested. Our risk assessment results (hazardous concentration, HC 5  = 281.6 mg Ce kg -1 ) support the idea that unintentional Ce input through P fertilizers does not pose a risk to soils of Brazilian agroecosystems., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Ammonia (NH 3 ) is a highly irritant, alkaline gas. Atmospheric emission of NH 3 was recognized as an environmental challenge. As a global issue, the NH 3 emission survey with spatially detailed information demonstrated that the sources of atmospheric NH 3 include agriculture (livestock wastes, fertilizers) and some industrial activities. As an environmental pollution, excessive NH 3 exposure can induce many bird dysfunction. Neutrophils respond to multiple invading pathogens through different mechanisms. In order to investigate the effect of NH 3 exposure on broilers' neutrophil, 1-day-old broilers were treated with/without NH 3 for 28 days. We extracted neutrophils from peripheral blood of chicken with/without NH 3 exposure and subsequently stimulated with PMA. Changes of cytokines and inflammatory bodies, heat shock proteins (HSPs), and glucose metabolism of neutrophil were examined in both cases. We not only explored that the index associated with inflammation changed due to NH 3 exposure but also observed the status of neutrophils which was treated with PMA stimulation. After NH 3 exposure, IL-1β and IL-6 were significantly increased on broilers neutrophil. Inflammatory-related factors (NLRP3, ASC, and caspase-1) were significantly elevated. The mRNA expression of HSP70 and HSP90 was increased significantly. All glucose metabolism indicators were reduced. In summary, we concluded that NH 3 enhanced inflammation and disrupted glucose metabolism, and increased the expression of HSPs and inflammatory factors. In addition, the sensitivity of neutrophils to exogenous stimuli was diminished. This information can not only be used to evaluate the damage of NH 3 -spiked neutrophils to chickens, but also provide clues for human health pathophysiology caused by excess NH 3 , providing valuable information for NH 3 risk management.

Antibiotic resistance genes (ARGs), prevalent across multiple environmental media, threaten human health worldwide and are considered emerging environmental contaminants. Earthworm gut, a niche for bacteria to survive, represents a potential reservoir for ARGs in soil. However, the compositions of ARGs in the earthworm gut microbiota remain elusive, especially under field conditions. In this study, we applied high-throughput quantitative PCR to profile the ARGs in the gut microbiota of earthworms after chronic exposure to fertilizers. To elucidate the factors that impact the ARGs composition, the bacterial community of gut microbiota, mobile genetic elements (MGEs), soil (nutrients, heavy metals, and antibiotics) and the properties of gut content (pH and nutrients) were analyzed. A total of 98 subtypes among 9 major types of ARGs, and 3 different MGEs were detected in the gut microbiota of earthworms. Organic fertilizer (sewage sludge and chicken manure) application significantly increased the diversity and abundance of ARGs. Of the 1123 identified operational taxonomic units (OTUs) at 97% similarity cutoff, most of them were assigned to Firmicutes (55.5%) and Proteobacteria (33.6%) in earthworm gut microbiota. Long-term organic fertilization slightly changed the microbiota composition, but did not impact the diversity. Partial redundancy analysis (pRDA) revealed that bacterial community, combined with environmental factors (soil and gut content properties) and MGEs, explained 72% of the variations of ARGs in the earthworm gut. Furthermore, the co-occurrence pattern between ARGs and MGEs indicated that horizontal gene transfer via MGEs may occur in the earthworm gut. These findings improve the current understanding of the dynamics of soil fauna-associated ARGs and the gut microbiota of earthworms may be an underappreciated hotspot for ARGs in the environment., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Eudrilus eugeniae, the vermicomposing worm, is found in considerable numbers in agricultural fields in India due to their eventual transfer through vermimanure. These worms are very often exposed to pesticides, herbicides, chemical fertilisers and other soil amendments. This paper reports the effects of variable concentrations of urea, phosphogypsum (PG), paper mill sludge (PMS) and two organophosphorus agrochemicals, monocrotophos and glyphosate, on certain morphological, histological and biochemical parameters of E. eugeniae. Results indicated setal anomalies, epidermal lesions, clitellar swelling and constriction of the body. Disintegration of connective tissue, vacuolation of dermis and significant alterations in protein, lipid peroxidation levels and activities of lactate dehydrogenase, acetylcholinesterase and catalase have also been observed in the treated worms. It is proposed that setae, connective tissue, protein and enzymes in E. eugeniae could be useful markers to evaluate toxicity due to the test chemicals.

Two field experiments were conducted at the experimental farm of the Faculty of Agriculture (Saba Basha), Abess Region, Alexandria University, Egypt, over two harvest seasons (2016/2017 and 2017/2018) to study the effect of a nanoparticle fertilizer (Hyper Feed Amino NPs) compound on productivity and genotoxicity in two wheat cultivars compared with conventional mineral fertilizer. Total yield, its components, and yield components were measured. After harvest, grains of both cultivars were collected and germinated to produce root tip. Mitosis division and chromosomal aberrations were observed. Mitotic indexes (%) were obtained from an analysis of about 2000 cells. Root tips of control treatments showed normal mitotic stages, and the mitotic index ranged from 61.67 ± 8.40 to 68.49 ± 11.23%. Although fertilization with NPK NPs produced an increase in yield, and root-tip cells showed various types of chromosomal aberrations such as multinuclei, micronuclei, abnormal metaphase, stickiness, nucleus erosion, nucleus elongation, fragments, c-metaphase, chromosome deletion, c-chromosome, gab chromosome, lagging chromosome, distributed anaphase, bridge, abnormal telophase, telophase with unequal division, cell membrane damage, cell elongation, and increased nuclear content. The 100% NPK NPs treatment produced 35.79 ± 9.32% and 38.98 ± 4.22% abnormal cells in Sids 12 and Al-Rasheed, respectively. Our results demonstrate that wheat root tip cells readily internalize NPK NPs which can interfere with normal cell function., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Cd is the third major contaminant of greatest hazard to the environment after mercury and lead and is considered as the only metal that poses health risks to both humans and animals at plant tissue concentrations that are generally not phytotoxic. Cd accumulation in plant shoots depends on Cd entry through the roots, sequestration within root vacuoles, translocation in the xylem and phloem, and Cd dilution within the plant shoot throughout its growth. Several metal transporters, processes, and channels are involved from the first step of Cd reaching the root cells and until its final accumulation in the edible parts of the plant. It is hard to demonstrate one step as the pivotal factor to decide the Cd tolerance or accumulation ability of plants since the role of a specific transporter/process varies among plant species and even cultivars. In this review, we discuss the sources of Cd pollutants, Cd toxicity to plants, and mechanisms of Cd uptake and redistribution in plant tissues. The metal transporters involved in Cd transport within plant tissues are also discussed and how their manipulation can control Cd uptake and/or translocation. Finally, we discuss the beneficial effects of Se on plants under Cd stress, and how it can minimize or mitigate Cd toxicity in plants.

Small-scale vegetable and fruit crop producers in the USA use locally available commercial organic fertilizers and soil amendments recycled from municipal and agricultural wastes. Organic soil amendments provide crops with their nutrient needs and maintain soil health by modifying its physical, chemical, and biological properties. However, organic soil amendments might add unwanted elements such as toxic heavy metals or salts, which might inhibit crop growth and reduce yield. Therefore, the objective of this study was to evaluate phytotoxicity of three commercial organic amendments, chicken manure, milorganite, and dairy manure, to collard greens using the seed germination bioassay and chemical analysis of the organic amendments. The seed germination bioassay was conducted by incubating collard greens seeds to germinate in 1:10 (w/v) organic amendment aqueous extracts. Results of this work identified phytotoxic effects of chicken manure and milorganite, but not dairy manure, to collard greens. Potentially phytotoxic chemicals such as copper, zinc, nickel, and salts were also higher in chicken manure and milorganite compared to dairy manure. In particular, nickel in chicken manure and milorganite aqueous extracts was 28-fold and 21-fold, respectively, higher than previously reported toxic levels to wheat seedlings. The results demonstrate the need for more research on phytotoxicity of commercial organic soil amendments to ensure their safe use in vegetable and fruit crop production systems.

Inadequate application of liquid pig manure (LPM) may pose risks to the soil due to the potential contaminants that exists, as well as by the large water input that can originate excessive moisture. By using Terrestrial Model Ecosystems, this study aimed to evaluate the effect of application of LPM (82% moisture) using the application rates of 20, 50 and 150 m 3 ha -1 and also of the corresponding amount of water to understand the origin of effects on the soil fauna of two sub-tropical soils. In general, the results obtained for the two soil types indicated that LPM (150 m 3 ha -1 ) changed the composition of soil fauna, with an increase in the abundance of insect larvae and dipterans, but a decrease in the number of earthworms and enchytraeids. Microbial biomass, soil respiration and the nutrients Mg, K and P increased with the amount of LPM supplied to the soil. When analysing the effects of adding large volumes of water via the LPM, results showed that application did not originate significant effects on the parameters measured. The differences obtained when comparing both treatments were mainly attributed to the organic load brought by the LPM and not to the amount of water without LPM., (Copyright © 2018 Elsevier Inc. All rights reserved.)

In recent years, increasing efforts have focused on production of organic-inorganic compound fertilizers using ammonium sulfite pulping waste liquor. However, their ecological effects on soil have not been studied. In this study, earthworm Eisenia fetida was exposed to various doses (0, 0.13, 0.26 and 0.52 kg/m 2 ) for different time (7, 14, 21, and 28 d) to evaluate the effects of fertilizers made from pulping waste liquor, including reactive oxygen species (ROS) generation, antioxidant enzymes activities, glutathione S-transferase enzyme (GST) activity, malondialdehyde (MDA) content and DNA damage. Results showed that there were significant increase of ROS and MDA levels after 14 d, inducing production of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT)) as well as GST. Before 14 d, excess ROS and MDA caused damage to the DNA of earthworms, leading to gradual increase of Olive tail moment (OTM) in the comet assay. With the exposure time extended to 28 d, owing to the combined effects of elimination of free radicals by antioxidant enzymes and detoxification enzymes as well as self-repairing function of cells, ROS and MDA levels declined slightly and OTM gradually decreased. In summary, this study indicated that there was a toxicological effect on earthworms when fertilizers made from pulping waste liquor were applied to soil, which needs more attention., (Copyright © 2018 Elsevier Inc. All rights reserved.)

The study evaluated source apportionment of heavy metals in vegetable samples from the potential sources of fertilizer, water and soil samples collected along the Changjiang River delta in China. The results showed that 25.72% of vegetable samples (Brassica chinensis L.) containing Pb, and Cd, Cu, Hg and Zn at relatively serious levels were from soil. Combined with principle component analysis (PCA) and cluster analysis (CA), the results of the spatial distribution of heavy metals in different environmental media indicated that fertilizer, water and soil were the main sources of heavy metals in vegetables. The results of multivariate linear regression (MLR) using partition indexes (P) showed that fertilizer contributed to 38.5%, 40.56%, 46.01%, 53.34% and 65.25% of As, Cd, Cu, Pb and Zn contents in vegetables, respectively. In contrast, 44.58% of As, 32.57% of Hg and 32.83% of Pb in vegetables came from soil and 42.78% of Cd and 66.97% of Hg contents in vegetables came from the irrigation water. The results of PCA and CA verified that MLR using P was suitable for determining source apportionment in a vegetable. A health risk assessment was performed; As, Cd and Pb contributed to more than 75% of the total hazard quotient (THQ) values and total carcinogenic risk values (Risk total ) for adults and children through oral ingestion. More than 70% of the estimated THQ and Risk total is contributed by water and fertilizer. Therefore, it is necessary to increase efforts in screening limits/levels of heavy metals in fertilizer and irrigation water and prioritize appropriate pollution management strategies., (Copyright © 2018 Elsevier Inc. All rights reserved.)

Sugarcane vinasse is a residue generated at a rate fifteen times greater than the ethanol production. Because of its high organic and micronutrient content, this residue is used as a fertilizer on sugarcane crops. However, when used in large quantities, vinasse can saturate the soil and contaminate nearby water resources by percolation and leaching. Given the proven toxic potential of in natura vinasse, the present study aimed to evaluate the toxic potential of leached sugarcane vinasse using Nile tilapia (Oreochromis niloticus) as a test organism. A bioassay was performed after vinasse percolation in laboratory soil columns. The bioassay included one control group containing fresh water and two treatment groups, the first exposed to a 2,5% dilution of leached of vinasse and the second to a 2,5% dilution of in natura vinasse. After exposure, histopathological analysis was performed in gills and livers, and the latter were labelled for HSP70 proteins. No significant changes were detected in the gills of the exposed fish. However, in the liver, both in natura and leached vinasse induced statistically significant histopathological changes. These changes include hydropic degeneration, cell boundary losses, pyknotic nuclei and cellular disorganization. HSP70 expression significant increase in liver of both treatment groups were observed, being higher for the in natura vinasse exposed group. Results suggested that both leached vinasse and in natura vinasse were toxic, its still able to provoke histological changes and induce the cytoprotective response in exposed fish liver, evidenced by a immunostaining of cellular stress proteins. Thus, in order to reduce its environmental impact, appropriated effluent disposal is essential., (Copyright © 2018. Published by Elsevier Inc.)

Several treatment technologies are available for the treatment of palm oil mill wastes. Vermicomposting is widely recognized as efficient, eco-friendly methods for converting organic waste materials to valuable products. This study evaluates the effect of different vermicompost extracts obtained from palm oil mill effluent (POME) and palm-pressed fiber (PPF) mixtures on the germination, growth, relative toxicity, and photosynthetic pigments of mung beans (Vigna radiata) plant. POME contains valuable nutrients and can be used as a liquid fertilizer for fertigation. Mung bean seeds were sown in petri dishes irrigated with different dilutions of vermicomposted POME-PPF extracts, namely 50, 60, and 70% at varying dilutions. Results showed that at lower dilutions, the vermicompost extracts showed favorable effects on seed germination, seedling growth, and total chlorophyll content in mung bean seedlings, but at higher dilutions, they showed inhibitory effects. The carotenoid contents also decreased with increased dilutions of POME-PPF. This study recommends that the extracts could serve as a good source of fertilizer for the germination and growth enhancement of mung bean seedlings at the recommended dilutions.

Drinking water treatment residuals (WTRs), the by-product of water clarification processes, are routinely disposed of via landfill however there is a growing body of research that demonstrates the material has great potential for beneficial use in environmental applications. Application to agricultural land is one option showing great promise (i.e. a low cost disposal route that provides organic matter input to soils and other potential benefits), however questions remain as to the impact such applications may have on earthworm survival and behaviour and also on the potential effects it may have on soil porewater chemistry. This study examined the leachability of elements within two types of WTRs (one Al- and one Fe- based) from England via 0.001 M CaCl 2 solution, at varying pH, and via the Community Bureau of Reference (BCR) sequential extraction scheme. Earthworm avoidance, survival, growth, reproduction and element concentrations were examined in WTR-amended sandy soils (0%, 5%, 10%, 20% w/w), while soil porewaters were also recovered from experimental units and examined for element concentrations. The results revealed leachable element concentrations to be very low in both types of WTRs tested and so element leaching from these WTRs would be unlikely to pose any threat to ecosystems under typical agricultural soil conditions. However, when the pH was lowered to 4.4 there was a substantial release of Al from the Al-WTRs (382 mg/kg). Soil porewater element concentrations were influenced to some degree by WTR addition, warranting further examination in terms of any potential implications for nutrient supply or limitation. Earthworm avoidance of WTR-amended soil was only observed for Al-WTRs and only at the maximum applied rate (20% w/w), while survival of earthworms was not affected by either WTR type at any application rate. Earthworm growth and reproduction (cocoon production) were not affected at a statistically significant level but this needs further examination over a longer period of exposure. Increased assimilation of Al and Fe into earthworm tissues was observed at some WTR application rates (maximum fresh weight concentrations of 42 mg/kg for Al and 167 mg/kg for Fe), but these were not at levels likely to pose environmental concerns., (Copyright © 2018 Elsevier Inc. All rights reserved.)

Insecticides and fertilizers are the most common agrochemicals that enter aquatic ecosystems from farmlands via different ways and consequently affect fish. In this study, effects of dimethoate, and Bacilar, as a bio-fertilizer, on oxidative stress and biochemical parameters were measured in common carp. For this purpose, fish were exposed to 16 and 32 μg L -1 dimethoate, 0.1 and 0.2 ml L -1 Bacilar and the mixtures of both compounds for 14 days. The results showed that Bacilar increased aspartate aminotransferase (AST) and LDH in gills, AST in liver and the activity of catalase (CAT) (0.2 ml L -1 ) in kidney, but decreased the activity of lactate dehydrogenase (LDH) and glucose 6-phsphate dehydrogenase (G6PDH) in liver. Dimethoate, alone or in combination with Bacilar, significantly increased LDH, alkaline phosphatase (ALP), AST and CAT activities in gills. Fish exposure to dimethoate, alone or in combination with Bacilar, resulted in a significant decrease in LDH, G6PDH activities, total antioxidant (TA) and glycogen levels in liver and TA in kidney. A significant increase was observed in malondialdehyde (MDA), ALP, AST, alanine aminotransferase (ALT) and CAT activity in liver and kidney of fish exposed to dimethoate alone or in combination with Bacilar. The results showed that cytotoxicity of Bacilar and dimethoate combination depended on their concentrations. Higher concentrations of Bacilar induced significant changes in some biochemical parameters. Dimethoate or/and Bacilar, in sub-lethal concentrations, induced oxidative damages in fish. Finally, these data support the hypothesis that changes in biochemical parameters were induced by exposure to dimethoate and/or Bacilar., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Phosphogypsum (PG) is the major solid waste generated by phosphate fertilizer plants and is used worldwide as sulfur and calcium supplement in agricultural soil. Considering the probability of elevated doses of PG during agricultural applications, this study was carried out to assess its impact on the connective tissue, tissue cholinesterase (ChE) activity, lactate dehydrogenase (LDH) activity, and lipid peroxidation (LPX) level of the tropical earthworm Glyphidrillus tuberosus (Stephenson) found in abundance in the rice fields in India. Consistent loss of connective tissue and sloughing of the intestinal epithelium were observed in worms exposed to 10%, 15%, and 20% concentrations of PG in soil over an incubation period of 30 days. ChE, LDH activities, and the level of LPX indicated highly significant variation (p < 0.01) between pre and postclitellar regions of the worm and concentrations of treatment. ChE activity was higher in postclitellar with respect to preclitellar region; however, the values for LDH activity and LPX level were higher in preclitellar region in comparison to postclitellar region in both PG treated and control worms. It was concluded that PG concentration at and beyond 10% could cause damage to muscle fibers and bring about significant alterations in these enzyme activities in G.tuberosus thus affecting the physiology and ecological functions of these worms.

The sequential extraction procedure of the Community Bureau of Reference (BCR) was applied to investigate the mobility of potentially toxic elements (As, Cd, Cr, and Pb) and nutrients (P, Ca, Mg, Cu, Fe, Mn, and Zn) in a multinutrient mineral fertilizer based on phosphate rocks supplemented with 10% (w w -1 ) micronutrient mixture (raw material used as a micronutrient source). For both samples, As and Cd were more mobile, whereas Cr remained in the solid residue. A higher mobility of Pb was observed in the micronutrient mixture; however, the high concentration of P (8.3% w w -1 ) in the fertilizer could have decreased Pb mobility as a result of Pb 3 (PO 4 ) 2 formation. The nutrients had great mobility, except Fe, which remained almost totally in the residual fraction in both samples. X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy analyses of solid residues showed that the ways in which elements were distributed in the sample particles could affect their mobility.

In this study, digestate from winery wastes was investigated focusing on phytotoxicity using macrophytes and evaluating the potential contribution of ammonium and copper. Spreading of digestate on soil could represent a suitable approach to recycle nutrients and organic matter, creating an on site circular economy. In this study, digestate quality was evaluated considering both chemical-physical characteristics and biological toxicity applying germination test. The effluent did not meet the entire amendment quality standard defined by Italian law (Decree 75/2010 germination index > 60% with solution of 30% v/v of digestate), but bio-stimulation was observed at low doses (3.15-6.25% v/v) for S. alba and S. saccharatum. The beneficial concentration agreed with Nitrate Directive dose and suggested that limited addition of digestate could have several positive effects on soil characteristics and on crop growth. Specific test using ammonium and copper solutions showed that these pollutants were not directly correlated to observed phytotoxicity., (Copyright © 2017 Elsevier Inc. All rights reserved.)

Surface waters are likely to be contaminated by both pesticides and fertilizers. Such contamination can result in changes in community composition if there is differential toxicity to individual taxa. We conducted a fully factorial mesocosm experiment that examined the single and interactive effects of environmentally realistic concentrations of nitrate and malathion on zooplankton communities and phytoplankton productivity. Malathion significantly decreased the abundance of total zooplankton, cyclopoid copepods, copepod nauplii, and Ceriodaphnia, and increased the abundance of rotifers. Nitrate addition generally had no effect on zooplankton; however, Ceriodaphnia abundance was higher in control mesocosms than in nitrate-treated mesocosms. There was only one significant interaction between malathion and nitrate treatments: For Ceriodaphnia, the no malathion, no nitrate mesocosms had much higher abundances than all other combinations of treatments. Without nitrate addition, chl a levels were uniformly low across all malathion treatments, whereas in the presence of nitrate, there were differences among the malathion treatments. In conclusion, our results demonstrate that malathion contamination of aquatic ecosystems can result in changes in the abundance and composition of zooplankton communities. In contrast, nitrate contamination appeared to have much less potential impact on zooplankton communities, either on its own or in interaction with malathion. Our results reinforce the notion that the effects of contaminants on aquatic ecosystems can be complex and further research examining the single and interactive effects of chemical stressors is needed to more fully understand their effects.

Bivalves are commonly used in biomonitoring programs to track pollutants. Several features, including its filter-feeding abilities, cumulatively argue in favour of the use of the Asian clam (Corbicula fluminea) as a biosentinel and an ecotoxicological model. Filtration in bivalves is very sensitive to external stimuli and its control is dictated by regulation of the opening/closure of the valves, which may be used as an avoidance defence against contaminants. Here, we investigate the filter-feeding behaviour of the Asian clam as an endpoint for assessing exposure to pollutants, driven by two complementary goals: (i) to generate relevant and sensitive toxicological information based on the ability of C. fluminea to clear an algal suspension, using the invasive species as a surrogate for native bivalves; (ii) to gain insight on the potential of exploring this integrative response in the refinement of chemical control methods for this pest. Clearance rates and proportion of algae removed were measured using a simple and reproducible protocol. Despite some variation across individuals and size classes, 50-90% of food particles were generally removed within 60-120 min by clams larger than 20 mm. Removal of algae was sensitive to an array of model contaminants with biocide potential, including fertilizers, pesticides, metals and salts: eight out of nine tested substances were detected at the μg l -1 or mg l -1 range and triggered valve closure, decreasing filter-feeding in a concentration-dependent manner. For most toxicants, a good agreement between mortality (96 h - LC 50 within the range 0.4-5500 mg l -1 ) and feeding (2 h - IC 50 within the range 0.005-2317 mg l -1 ) was observed, demonstrating that a 120-min assay can be used as a protective surrogate of acute toxicity. However, copper sulphate was very strongly avoided by the clams (IC 50  = 5.3 μg l -1 ); on the contrary, dichlorvos (an organophosphate insecticide) did not cause feeding depression, either by being undetected by the clams' chemosensors and/or by interfering with the valve closure mechanism. Such an assay has a large potential as a simple screening tool for industry, environmental agencies and managers. The ability of dichlorvos to bypass the Asian clam's avoidance strategy puts it in the spotlight as a potential agent to be used alone or combined with others in eradication programs of this biofouler in closed or semi-closed industrial settings., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Schistosomiasis is a snail-borne parasitic disease that ranks among the most important water-based diseases of humans in developing countries. Increased prevalence and spread of human schistosomiasis to non-endemic areas has been consistently linked with water resource management related to agricultural expansion. However, the role of agrochemical pollution in human schistosome transmission remains unexplored, despite strong evidence of agrochemicals increasing snail-borne diseases of wildlife and a projected 2- to 5-fold increase in global agrochemical use by 2050. Using a field mesocosm experiment, we show that environmentally relevant concentrations of fertilizer, a herbicide, and an insecticide, individually and as mixtures, increase densities of schistosome-infected snails by increasing the algae snails eat and decreasing densities of snail predators. Epidemiological models indicate that these agrochemical effects can increase transmission of schistosomes. Identifying agricultural practices or agrochemicals that minimize disease risk will be critical to meeting growing food demands while improving human wellbeing.

Nitrogen (N) deposition due to anthropogenic pollution is a major driver of the global biodiversity loss. We studied the effect of experimental N and phosphorus (P) fertilization (0, 10, 20, and 50 kg N ha -1 year -1 and 14 kg P ha -1  year -1 over the background deposition levels) on plant cover dynamics of a rosemary (Rosmarinus officinalis L.) shrubland after 8 years of nutrient addition in a semiarid Mediterranean ecosystem from Central Spain. We specifically aimed at testing whether N deposition has the potential to influence the observed expanding trend of woody vegetation into areas dominated by grassland, biological soil crusts, and bare soil. Our results show that N addition loads above 10 kg N ha -1 year -1 reverted the cover dynamics of shrubs. Under N addition conditions, N was no longer a limiting nutrient and other elements, especially P and calcium, determined the seasonal growth of young twigs. Interestingly, N fertilization did not inhibit the growth of young shoots; our estimates point to a reduced rosemary leaf lifespan that is driving individuals to death. This may be triggered by long-term accumulation of N compounds in leaves, suggesting the need to consider the old organs and tissues in long-lived perennial plants, where N toxicity effects could be more mediated by accumulation processes. Shrublands are a widely distributed ecosystem type in biodiverse Mediterranean landscapes, where shrubs play a key role as nurse plants. Therefore, the disappearance of shrublands may accelerate the biodiversity loss associated with other global change drivers, hamper the recruitment of seedlings of woody species, and, as a consequence, accelerate desertification.

Nanoparticles with their unique surface properties can modulate the physiological, biochemical, and physicochemical pathways, such as photosynthesis, respiration, nitrogen metabolism, and solute transport. In this context, researchers have developed a wide range of engineered nanomaterials (ENMs) for the improvement of growth and productivity by modulating the metabolic pathways in plants. This class of tailor-made materials can potentially lead to the development of a new group of agrochemical nanofertilizers. However, there are reports that engineered nanomaterials could impart phytotoxicity to edible and medicinal plants. On the contrary, there is a series of ENMs that might be detrimental when applied directly and/or indirectly to the plants. These particles can sometimes readily aggregate and dissolute in the immediate vicinity; the free ions released from the nanomatrix can cause serious tissue injury and membrane dysfunction to the plant cell through oxidative stress. On that note, thorough studies on uptake, translocation, internalization, and nutritional quality assessment must be carried out to understand ENM-plant interactions. This review critically discusses the possible beneficial or adverse aftereffect of nanofertilizers in the immediate environment to interrelate the impacts of ENMs on the crop health and food security management.

Phosphate fertilizer industry is considered as one of the main sources of environmental pollutants. Besides solid waste products, e.g. phosphates, sulphates, and heavy metals, also atmospheric pollutants, such as hydrofluoric acid fumes (HF), sulphur dioxide (SO 2 ), nitrogen oxides (NO 2 ), and particulate matter with diameter up to 10 μm (PM 10 ) can be dangerous. Genotoxic effect of these pollutants was monitored by assessing the DNA damage using alkaline comet assay on cells from brain, thoracic muscles and gut of Aiolopus thalassinus collected at three sites (A-C) located at 1, 3, and 6 km away from Abu-Zaabal Company for Fertilizers and Chemical Industries. Control site was established 32 km from the source of pollution, at the Cairo University Campus. The level of the DNA damage was significantly higher in insects from polluted sites comparing to that from the control site. A strong negative correlation between percentage of cells with visible DNA damage (% of severed cells) and the distance of the sites from Abu-Zaabal Company was found. The best parameter for monitoring of fertilizer pollutants is % of severed cells. Possible impact of Abu-Zaabal Company (extremely high concentration of phosphates and sulphates in all the polluted sites) on DNA integrity in A. thalassinus tissues was discussed. The potential use of the comet assay as a biomonitoring method of the environmental pollution caused by fertilizer industry was proposed. Specific pollution resulting from the activity of the fertilizer industry can cause comparable adverse effects in the organisms inhabiting areas up to 6 km from the source of contamination., (Copyright © 2017 Elsevier Ltd. All rights reserved.)