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170 results on '"Ferruzzi, Guido"'

2. Determination of ochratoxin A in pig tissues using enzymatic digestion coupled with high-performance liquid chromatography with a fluorescence detector

Author

Ferruzzi Guido, Mani Danilo, Intorre Luigi, Meucci Valentina, Vanni Michele, and Luci Giacomo

Subjects
Ochratoxin A, Veterinary Science and Veterinary Medicine, Clinical Biochemistry, Enzyme assisted digestion coupled to HPLC-FLD, 02 engineering and technology, Kidney, 01 natural sciences, High-performance liquid chromatography, Fluorescence spectroscopy, chemistry.chemical_compound, ComputingMethodologies_COMPUTERGRAPHICS, Enzyme assisted digestion, Pig, Chromatography, Enzymatic digestion, Chemistry, Liver and kidney, 010401 analytical chemistry, Extraction (chemistry), Repeatability, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Medical Laboratory Technology, Liver, Pork meat, Muscle, 0210 nano-technology
Abstract

Graphical abstract, We present a new method for the rapid analysis of ochratoxin A (OTA) in pig tissues (muscle, liver and kidney) using enzymatic digestion (ED) coupled to high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). OTA was digested with a 1% pancreatin solution in a phosphate buffer and then cleaned with ethylacetate. After being evaporated to dryness and re-dissolved, the sample was determined using HPLC-FLD. The method was validated taking into account the currently permitted limit of 1 μg/kg OTA in pork meat and derived products in Italy. The recovery was higher than 90%. Intra- and inter-day repeatability expressed as RSD were less than 7%. The LOD and LOQ were 0.001 and 0.002 μg/kg, respectively. Our method is more efficient, easier, and cheaper than conventional clean-up procedures (liquid–liquid extraction). • The aim of the study was to develop and validate a quantitative HPLC-FLD method based on ED followed by a chromatographic analysis without any previous clean-up or concentration step for the detection of OTA in pig tissues. • The ED method showed a 90%+ recovery, and intra- and inter-day RSD less than 7%. • This method is simple, rapid, easy to use, and consumes low amounts of organic solvents.

Published
2016
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5. Determination of ochratoxin A in tissues of wild boar (Sus scrofa L.) by enzymatic digestion (ED) coupled to high-performance liquid chromatography with a fluorescence detector (HPLC-FLD)

Author

Luci, Giacomo, primary, Intorre, Luigi, additional, Ferruzzi, Guido, additional, Mani, Danilo, additional, Giuliotti, Lorella, additional, Pretti, Carlo, additional, Tognetti, Rosalba, additional, Bertini, Simone, additional, and Meucci, Valentina, additional

Published
2017
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6. Environmental impact caused by wild ungulates in protected areas

Author

Pistoia, Alessandro, Bertolotto, P., Tomei, R., Poli, Piera, Casarosa, Laura, and Ferruzzi, Guido

Subjects
Environmental impact, plants biodiversity, Environmental impact, plants biodiversity, protected areas, feral goat, wild boar, feral goat, protected areas, wild boar
Published
2015

9. Acidic composition of gilthead seabream fillets (Sparus aurata L.) fed with different lipid sources: preliminary evaluation

Author

Berni, Paolo, Ferruzzi, Guido, Casarosa, Laura, Pistoia, Alessandro, and Balestri, G.

Subjects
lipids (amino acids, peptides, and proteins), Sparus aurata L., fatty acid, vegetable oils, lcsh:Animal culture, lcsh:SF1-1100
Abstract

Dietary lipids play an important role in fish nutrition as a source provision of body energy and essential fatty acids (EFA) (Sargent et al., 1999). The low availability and the high exspense of fish oil could suggest the use of an alternative lipid source in aquaculture (Turchini et al., 2000). Alternative sources of PUFA are represented by vegetable oils, but their composition is not satisfactory for the requirements of the different species of rearing fishes...

Published
2011

12. Determination of ochratoxin A in tissues of wild boar (<italic>Sus scrofa</italic> L.) by enzymatic digestion (ED) coupled to high-performance liquid chromatography with a fluorescence detector (HPLC-FLD).

Author

Luci, Giacomo, Intorre, Luigi, Ferruzzi, Guido, Mani, Danilo, Giuliotti, Lorella, Pretti, Carlo, Tognetti, Rosalba, Bertini, Simone, and Meucci, Valentina

Abstract

Ochratoxin A (OTA) is a secondary toxic metabolite synthesized by Aspergillus or Penicillium species, which can contaminate various crops. The International Agency for Research on Cancer (IARC) classified OTA as a group 2B possible human carcinogen. The aim of the present study was to assess OTA concentrations in tissues of wild boar (Sus scrofa L.) from Tuscany (Italy). Over a period of 2 years, samples of muscle, liver, and kidney from 48 wild boars were collected and concentrations of OTA were determined by enzymatic digestion (ED) coupled to high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). The highest concentrations of OTA were found in the kidneys of the 48 wild boars analyzed. No difference in concentrations was found based on years of collection and sex while a significantly higher OTA concentration was found in the kidney of the young wild boars with respect to the adult one. Monitoring the quality of meat destined for transformation is a priority in order to decrease the possibility of toxin carry-over to humans. The present study showed that contamination of wild boar meat products by OTA represents a potential emerging source of OTA. [ABSTRACT FROM AUTHOR]

Published
2018
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