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1. The Italian law on body donation: A position paper of the Italian College of Anatomists

Author

De Caro, Raffaele, Boscolo-Berto, Rafael, Artico, Marco, Bertelli, Eugenio, Cannas, Mario, Cappello, Francesco, Carpino, Guido, Castorina, Sergio, Cataldi, Amelia, Cavaletti, Guido Angelo, Cinti, Saverio, Cocco, Lucio Ildebrando, Cremona, Ottavio, Crivellato, Enrico, De Luca, Antonio, Falconi, Mirella, Familiari, Giuseppe, Ferri, Gian Luca, Fornai, Francesco, Gesi, Marco, Geuna, Stefano, Gibelli, Daniele Maria, Giordano, Antonio, Gobbi, Pietro, Guerra, Germano, Gulisano, Massimo, Macchi, Veronica, Macchiarelli, Guido, Manzoli, Lucia, Michetti, Fabrizio, Miscia, Sebastiano, Montagnani, Stefania, Montella, Andrea Costantino Mario, Morini, Sergio, Onori, Paolo, Palumbo, Carla, Papa, Michele, Porzionato, Andrea, Quacci, Daniela Elena, Raspanti, Mario, Rende, Mario, Rezzani, Rita, Ribatti, Domenico, Ripani, Maurizio, Rodella, Luigi Fabrizio, Rossi, Pellegrino, Sbarbati, Andrea, Secchiero, Paola, Sforza, Chiarella, Stecco, Carla, Toni, Roberto, Vercelli, Alessandro, Vitale, Marco, Zancanaro, Carlo, Zauli, Giorgio, Zecchi, Sandra, Anastasi, Giuseppe Pio, and Gaudio, Eugenio

Published
2021
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2. Telomere attrition and inflammatory load in severe psychiatric disorders and in response to psychotropic medications

Author

Squassina, Alessio, Manchia, Mirko, Pisanu, Claudia, Ardau, Raffaella, Arzedi, Carlo, Bocchetta, Alberto, Caria, Paola, Cocco, Cristina, Congiu, Donatella, Cossu, Eleonora, Dettori, Tinuccia, Frau, Daniela Virginia, Garzilli, Mario, Manca, Elias, Meloni, Anna, Montis, Maria Antonietta, Mura, Andrea, Nieddu, Mariella, Noli, Barbara, Paribello, Pasquale, Pinna, Federica, Robledo, Renato, Severino, Giovanni, Sogos, Valeria, Del Zompo, Maria, Ferri, Gian Luca, Chillotti, Caterina, Vanni, Roberta, and Carpiniello, Bernardo

Published
2020
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4. Brain-reactive autoantibodies in neuropsychiatric systemic lupus erythematosus

Author

Cocco, Cristina, primary, Manca, Elias, additional, Corda, Giulia, additional, Angioni, Maria Maddalena, additional, Noli, Barbara, additional, Congia, Mattia, additional, Loy, Francesco, additional, Isola, Michela, additional, Chessa, Elisabetta, additional, Floris, Alberto, additional, Lorefice, Lorena, additional, Saba, Luca, additional, Mathieu, Alessandro, additional, Ferri, Gian Luca, additional, Cauli, Alberto, additional, and Piga, Matteo, additional

Published
2023
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5. Identification of novel cerebrospinal fluid biomarker candidates for dementia with Lewy bodies: a proteomic approach

Author

van Steenoven, Inger, Koel-Simmelink, Marleen J. A., Vergouw, Leonie J. M., Tijms, Betty M., Piersma, Sander R., Pham, Thang V., Bridel, Claire, Ferri, Gian-Luca, Cocco, Cristina, Noli, Barbara, Worley, Paul F., Xiao, Mei-Fang, Xu, Desheng, Oeckl, Patrick, Otto, Markus, van der Flier, Wiesje M., de Jong, Frank Jan, Jimenez, Connie R., Lemstra, Afina W., and Teunissen, Charlotte E.

Published
2020
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8. Erratum:Correction: Boiten et al. Pathologically Decreased CSF Levels of Synaptic Marker NPTX2 in DLB Are Correlated with Levels of Alpha-Synuclein and VGF. Cells 2021, 10, 38 (Cells (2020) 10 1 PII: 652)

Author

Boiten, Walter A., van Steenoven, Inger, Xiao, Mei-Fang, Worley, Paul F., Noli, Barbara, Cocco, Cristina, Ferri, Gian-Luca, Lemstra, Afina W., Teunissen, Charlotte E., Anatomy and neurosciences, Neurology, Amsterdam Neuroscience - Neurodegeneration, Clinical chemistry, and Amsterdam Neuroscience - Neuroinfection & -inflammation

Abstract

The authors want to notify that they have made the following changes to the author list of the paper [...].

Published
2022

11. TLQP-21 changes in response to a glucose load

Author

Corda, Giulia, primary, Noli, Barbara, additional, Manconi, Barbara, additional, Brancia, Carla, additional, Pellegrini, Manuela, additional, Naro, Fabio, additional, Olianas, Alessandra, additional, Ferri, Gian-Luca, additional, and Cocco, Cristina, additional

Published
2021
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12. BTK inhibitors synergise with 5-FU to treat drug-resistant TP53-null colon cancers

Author

Lavitrano, M, Ianzano, L, Bonomo, S, Cialdella, A, Cerrito, M, Pisano, F, Missaglia, C, Giovannoni, R, Romano, G, Mclean, C, Voest, E, D'Amato, F, Noli, B, Ferri, G, Agostini, M, Pucciarelli, S, Helin, K, Leone, B, Canzonieri, V, Grassilli, E, Lavitrano, Marialuisa, Ianzano, Leonarda, Bonomo, Sara, Cialdella, Annamaria, Cerrito, Maria Grazia, Pisano, Fabio, Missaglia, Carola, Giovannoni, Roberto, Romano, Gabriele, McLean, Chelsea M, Voest, Emile E, D'Amato, Filomena, NOLI, BARBARA, Ferri, Gian Luca, Agostini, Marco, Pucciarelli, Salvatore, Helin, Kristian, Leone, Biagio Eugenio, Canzonieri, Vincenzo, Grassilli, Emanuela, Lavitrano, M, Ianzano, L, Bonomo, S, Cialdella, A, Cerrito, M, Pisano, F, Missaglia, C, Giovannoni, R, Romano, G, Mclean, C, Voest, E, D'Amato, F, Noli, B, Ferri, G, Agostini, M, Pucciarelli, S, Helin, K, Leone, B, Canzonieri, V, Grassilli, E, Lavitrano, Marialuisa, Ianzano, Leonarda, Bonomo, Sara, Cialdella, Annamaria, Cerrito, Maria Grazia, Pisano, Fabio, Missaglia, Carola, Giovannoni, Roberto, Romano, Gabriele, McLean, Chelsea M, Voest, Emile E, D'Amato, Filomena, NOLI, BARBARA, Ferri, Gian Luca, Agostini, Marco, Pucciarelli, Salvatore, Helin, Kristian, Leone, Biagio Eugenio, Canzonieri, Vincenzo, and Grassilli, Emanuela

Abstract

Colorectal cancer (CRC) is the fourth cause of death from cancer worldwide mainly due to the high incidence of drug-resistance. During a screen for new actionable targets in drug-resistant tumours we recently identified p65BTK – a novel oncogenic isoform of Bruton's tyrosine kinase. Studying three different cohorts of patients here we show that p65BTK expression correlates with histotype and cancer progression. Using drug-resistant TP53-null colon cancer cells as a model we demonstrated that p65BTK silencing or chemical inhibition overcame the 5-fluorouracil resistance of CRC cell lines and patient-derived organoids and significantly reduced the growth of xenografted tumours. Mechanistically, we show that blocking p65BTK in drug-resistant cells abolished a 5-FU-elicited TGFB1 protective response and triggered E2F-dependent apoptosis. Taken together, our data demonstrated that targeting p65BTK restores the apoptotic response to chemotherapy of drug-resistant CRCs and gives a proof-of-concept for suggesting the use of BTK inhibitors in combination with 5-FU as a novel therapeutic approach in CRC patients. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Published
2020

16. Differences in telomere length between patients with bipolar disorder and controls are influenced by lithium treatment

Author

Pisanu, Claudia, primary, Congiu, Donatella, additional, Manchia, Mirko, additional, Caria, Paola, additional, Cocco, Cristina, additional, Dettori, Tinuccia, additional, Frau, Daniela Virginia, additional, Manca, Elias, additional, Meloni, Anna, additional, Nieddu, Mariella, additional, Noli, Barbara, additional, Pinna, Federica, additional, Robledo, Renato, additional, Sogos, Valeria, additional, Ferri, Gian Luca, additional, Carpiniello, Bernardo, additional, Vanni, Roberta, additional, Bocchetta, Alberto, additional, Severino, Giovanni, additional, Ardau, Raffaella, additional, Chillotti, Caterina, additional, Zompo, Maria Del, additional, and Squassina, Alessio, additional

Published
2020
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17. P17 Anti-neuronal antibodies in patients with systemic lupus erythematosus: prevalence, clinical associations and searching for a specific target

Author

Chessa, Elisabetta, primary, Angioni, Maria Maddalena, additional, Pinna, Silvia, additional, Congia, Mattia, additional, Floris, Alberto, additional, Manca, Elias, additional, Corda, Giulia, additional, Lorefice, Lorena, additional, Ferri, Gian Luca, additional, Cocco, Cristina, additional, Mathieu, Alessandro, additional, Cauli, Alberto, additional, and Piga, Matteo, additional

Published
2020
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18. A multidisciplinary approach to mental illness: do inflammation, telomere length and microbiota form a loop? A protocol for a cross-sectional study on the complex relationship between inflammation, telomere length, gut microbiota and psychiatric disorders

Author

Manchia, Mirko, primary, Paribello, Pasquale, additional, Arzedi, Carlo, additional, Bocchetta, Alberto, additional, Caria, Paola, additional, Cocco, Cristina, additional, Congiu, Donatella, additional, Cossu, Eleonora, additional, Dettori, Tinuccia, additional, Frau, Daniela V, additional, Garzilli, Mario, additional, Manca, Elias, additional, Meloni, Anna, additional, Montis, Maria A, additional, Mura, Andrea, additional, Nieddu, Mariella, additional, Noli, Barbara, additional, Pinna, Federica, additional, Pisanu, Claudia, additional, Robledo, Renato, additional, Severino, Giovanni, additional, Sogos, Valeria, additional, Chillotti, Caterina, additional, Carpiniello, Bernardo, additional, Del Zompo, Maria, additional, Ferri, Gian Luca, additional, Vanni, Roberta, additional, and Squassina, Alessio, additional

Published
2020
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20. BTK inhibitors synergise with 5‐FU to treat drug‐resistant TP53 ‐null colon cancers

Author

Lavitrano, Marialuisa, primary, Ianzano, Leonarda, additional, Bonomo, Sara, additional, Cialdella, Annamaria, additional, Cerrito, Maria Grazia, additional, Pisano, Fabio, additional, Missaglia, Carola, additional, Giovannoni, Roberto, additional, Romano, Gabriele, additional, McLean, Chelsea M, additional, Voest, Emile E, additional, D'Amato, Filomena, additional, Noli, Barbara, additional, Ferri, Gian Luca, additional, Agostini, Marco, additional, Pucciarelli, Salvatore, additional, Helin, Kristian, additional, Leone, Biagio E, additional, Canzonieri, Vincenzo, additional, and Grassilli, Emanuela, additional

Published
2019
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21. VGF peptides as novel biomarkers in Parkinson’s disease

Author

Cocco, Cristina, primary, Corda, Giulia, additional, Lisci, Carlo, additional, Noli, Barbara, additional, Carta, Manolo, additional, Brancia, Carla, additional, Manca, Elias, additional, Masala, Carla, additional, Marrosu, Francesco, additional, Solla, Paolo, additional, Manconi, Barbara, additional, Bongioanni, Paolo, additional, and Ferri, Gian-Luca, additional

Published
2019
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26. p65BTK is a novel potential actionable target in KRAS-mutated/EGFR-wild type lung adenocarcinoma

Author

Giordano, F, Vaira, V, Cortinovis, D, Bonomo, S, Goedmakers, J, Brena, F, Cialdella, A, Ianzano, L, Forno, I, Cerrito, M, Giovannoni, R, Ferri, G, Tasciotti, E, Vicent, S, Damarco, F, Bosari, S, Lavitrano, M, Grassilli, E, Giordano, Federica, Vaira, Valentina, Cortinovis, Diego, Bonomo, Sara, Goedmakers, Joyce, Brena, Federica, Cialdella, Annamaria, Ianzano, Leonarda, Forno, Irene, Cerrito, Maria Grazia, Giovannoni, Roberto, Ferri, Gian Luca, Tasciotti, Ennio, Vicent, Silve, Damarco, Francesco, Bosari, Silvano, Lavitrano, Marialuisa, Grassilli, Emanuela, Giordano, F, Vaira, V, Cortinovis, D, Bonomo, S, Goedmakers, J, Brena, F, Cialdella, A, Ianzano, L, Forno, I, Cerrito, M, Giovannoni, R, Ferri, G, Tasciotti, E, Vicent, S, Damarco, F, Bosari, S, Lavitrano, M, Grassilli, E, Giordano, Federica, Vaira, Valentina, Cortinovis, Diego, Bonomo, Sara, Goedmakers, Joyce, Brena, Federica, Cialdella, Annamaria, Ianzano, Leonarda, Forno, Irene, Cerrito, Maria Grazia, Giovannoni, Roberto, Ferri, Gian Luca, Tasciotti, Ennio, Vicent, Silve, Damarco, Francesco, Bosari, Silvano, Lavitrano, Marialuisa, and Grassilli, Emanuela

Abstract

Background: Lung cancer is still the main cause of cancer death worldwide despite the availability of targeted therapies and immune-checkpoint inhibitors combined with chemotherapy. Cancer cell heterogeneity and primary or acquired resistance mechanisms cause the elusive behaviour of this cancer and new biomarkers and active drugs are urgently needed to overcome these limitations. p65BTK, a novel isoform of the Bruton Tyrosine Kinase may represent a new actionable target in non-small cell lung cancer (NSCLC). Methods: p65BTK expression was evaluated by immunohistochemistry in 382 NSCLC patients with complete clinico-pathological records including smoking habit, ALK and EGFR status, and in metastatic lymph nodes of 30 NSCLC patients. NSCLC cell lines mutated for p53 and/or a component of the RAS/MAPK pathway and primary lung cancer-derived cells from Kras/Trp53 null mice were used as a preclinical model. The effects of p65BTK inhibition by BTK Tyrosine Kinase Inhibitors (TKIs) (Ibrutinib, AVL-292, RN486) and first-generation EGFR-TKIs (Gefitinib, Erlotinib) on cell viability were evaluated by MTT. The effects of BTK-TKIs on cell growth and clonogenicity were assessed by crystal violet and colony assays, respectively. Cell toxicity assays were performed to study the effect of the combination of non-toxic concentrations of BTK-TKIs with EGFR-TKIs and standard-of-care (SOC) chemotherapy (Cisplatin, Gemcitabine, Pemetrexed). Results: p65BTK was significantly over-expressed in EGFR-wild type (wt) adenocarcinomas (AdC) from non-smoker patients and its expression was also preserved at the metastatic site. p65BTK was also over-expressed in cell lines mutated for KRAS or for a component of the RAS/MAPK pathway and in tumors from Kras/Trp53 null mice. BTK-TKIs were more effective than EGFR-TKIs in decreasing cancer cell viability and significantly impaired cell proliferation and clonogenicity. Moreover, non-toxic doses of BTK-TKIs re-sensitized drug-resistant NSCLC cell lines

Published
2019

30. T44MULTIDISCIPLINARY APPROACH TO MENTAL ILLNESS: DO INFLAMMATION AND TELOMERE LENGTH FORM A LOOP?

Author

Pisanu, Claudia, primary, Caria, Paola, additional, Carpiniello, Bernardo, additional, Chillotti, Caterina, additional, Cocco, Cristina, additional, Dettori, Tinuccia, additional, Ferri, Gian Luca, additional, Kordou, Zoi, additional, Manchia, Mirko, additional, Noli, Barbara, additional, Pinna, Federica, additional, Stamopoulou, Theano, additional, Tsermpini, Evangelia Eirini, additional, Vanni, Roberta, additional, and Squassina, Alessio, additional

Published
2019
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31. VGF Peptides in Cerebrospinal Fluid of Patients with Dementia with Lewy Bodies

Author

van Steenoven, Inger, primary, Noli, Barbara, additional, Cocco, Cristina, additional, Ferri, Gian-Luca, additional, Oeckl, Patrick, additional, Otto, Markus, additional, Koel-Simmelink, Marleen J. A., additional, Bridel, Claire, additional, van der Flier, Wiesje M., additional, Lemstra, Afina W., additional, and Teunissen, Charlotte E., additional

Published
2019
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32. Photoperiodic changes in adiposity increase sensitivity of female Siberian hamsters to systemic VGF derived peptide TLQP-21

Author

Lisci, Carlo, primary, Lewis, Jo E., additional, Daniel, Zoe C. T. R., additional, Stevenson, Tyler J., additional, Monnier, Chloe, additional, Marshall, Hayley J., additional, Fowler, Maxine, additional, Ebling, Francis J. P., additional, Ferri, Gian-Luca, additional, Cocco, Cristina, additional, and Jethwa, Preeti H., additional

Published
2019
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33. Changes of TLQP-21 in Response to Glucose Load

Author

Corda, Giulia, primary, Noli, Barbara, additional, Manconi, Barbara, additional, Brancia, Carla, additional, Pellegrini, Manuela, additional, Naro, Fabio, additional, Ferri, Gian-Luca, additional, and Cocco, Cristina, additional

Published
2019
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35. Neuronal Antibodies and Brain alterations in APECED Patients

Author

Corda, Giulia, Meloni, Antonella, Saba, Luca, Mariotti, Stefano, Ferri, Gian-Luca, and Cocco, Cristina

Subjects
APECED, Autoantibodies, dopaminergic cells, GABAergic cells
Abstract

APECED (Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Distrophy) is a rare autosomal recessive disorder. We previously found that sera samples from 9/14 patients revealed autoantibodies (Auto-Abs) reacting with cerebellum (GABAergic cells, n=5) and substantia nigra (SN; dopaminergic cells, n=5) [1]. Follow-up of the large majority of these patients was perfomed at least 10 years after the previous investigation. Indeed, on these patients, and on control age-matched subjects (n=14), we performed brain examinations using an MRI scanner. Obtained images were used to evaluate the volumes of white and gray matter (W.M and G.M., respectively) as well as the ventricles (III and IV). In addition, we used immunohistochemistry (IHC) on tissues from rat brain (after perfusion with 4% paraformaldehyde) in order to confirm the previous immunoreactivities or found new Auto-Abs cell targets. The brain MR revealed a reduction of G.M (p = 0.042) and cerebellum (p = 0.0012), and an increase of ventricles (p = 0.0001), compared to controls. Through IHC, after 10 years, we found 11/14 patients producing Auto-Abs against different brain neuronal cells. In detail, among the patients previously investigated and containing Auto-Abs against GABAergic perikarya in the cerebellum, 3 still contained the same immunoreactivity while 1 was unavailable, and 1 lost the reactivity. Instead, as to Auto-Abs against dopaminergic perikarya in the SN, 4 patients confirmed their previous reactivity, while 3 previously negative patients, revealed novel positivity (in total, n=7). A new immunoreactivity against the 5HT cells in the brainstem were also revealed in the same patients with Auto-Abs to SN (n=7). In conclusion, the co-presence of brain volume changes and neuronal Auto-Abs in APECED patients could suggest an autoimmune manifestation at the brain level that should be taken in consideration., Italian Journal of Anatomy and Embryology, Vol. 122, No. 1 (Supplement) 2017

Published
2017
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36. TLQP peptides in Amyotrophic Lateral Sclerosis

Author

Brancia, Carla, Noli, Barbara, Boido, Marina, Vercelli, Alessandro, Bongioanni, Paolo, Ferri, Gian-Luca, and Cocco, Cristina

Subjects
ALS, VGF, TLQP peptides, motor neurons
Abstract

TLQP peptides (TLQPp) act on synaptic strengthening or against neuronal apoptosis but their involvement in Amyotrophic Lateral Sclerosis (ALS) is not well known. We used an ALS animal model (SOD1-G93A) and human tissues (plasma and fibroblasts from both patients and controls), to address the TLQPp expression and their modulation in ALS mechanisms. Mouse motor neuronal cells (NSC-34), were used to study the neuroprotective role of the TLQP-21 against oxidative stress induced by Sodium Arsenite. TLQPp were immunoquantified by ELISA, their cell expression studied by immunofluorescence while cell viability was addressed by MTT assay. In the NSC-34 naïve cells, TLQPp were found within the cytoplasm, axons and growth cones. Upon stress, they appeared immunolocalized exclusively within a cytoplasmatic area, close to the nucleus, (probably the Golgi). Moreover, under stress, TLQPp levels were reduced (42%, p=3.1x10-6), while the exogenous increase of TLQP-21 improved cell viability (13%, p= 0.018). In naïve fibroblasts, TLQPp were also similarly localized in an area, likely the Golgi, of patients and controls while their reduction (31%, p=0.03) was observed in cells with TARDBP-A382T mutation. Stress granules only appeared after SA treatment, and did not contain TLQPp. In plasma, a reduced release of TLQPp was associated with the beginning of the clinical motor symptoms in patients (12%, p=0.026), and with a pre-symptomatic stage in mutant mice (26.3%, p=0.048). Finally, in mouse spinal cord we identified by vescicular acetylcholine transporter (VAChT) antibody, a specific localization of the TLQPp in the motor neurons with a reduction in the pre-symptomatic stage of mutant mice. In conclusion, TLQPp are reduced in the stressed NSC-34 cells, mutant mouse motor neurons as well as in fibroblasts with TARDBP-A382T mutation that also induces production of reactive oxygen specie. Hence, we suggest that their reduction may occur in response to oxidative stress. They could be also considered as early biomarkers, while TLQP-21 acts as neuroprotective factor., Italian Journal of Anatomy and Embryology, Vol. 122, No. 1 (Supplement) 2017

Published
2017
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37. BTK inhibitors synergise with 5‐FU to treat drug‐resistant TP53‐null colon cancers.

Author

Lavitrano, Marialuisa, Ianzano, Leonarda, Bonomo, Sara, Cialdella, Annamaria, Cerrito, Maria Grazia, Pisano, Fabio, Missaglia, Carola, Giovannoni, Roberto, Romano, Gabriele, McLean, Chelsea M, Voest, Emile E, D'Amato, Filomena, Noli, Barbara, Ferri, Gian Luca, Agostini, Marco, Pucciarelli, Salvatore, Helin, Kristian, Leone, Biagio E, Canzonieri, Vincenzo, and Grassilli, Emanuela

Subjects
COLON cancer, FLUOROURACIL, PROTEIN-tyrosine kinases, CANCER invasiveness, CANCER cells
Abstract

Colorectal cancer (CRC) is the fourth cause of death from cancer worldwide mainly due to the high incidence of drug‐resistance. During a screen for new actionable targets in drug‐resistant tumours we recently identified p65BTK – a novel oncogenic isoform of Bruton's tyrosine kinase. Studying three different cohorts of patients here we show that p65BTK expression correlates with histotype and cancer progression. Using drug‐resistant TP53‐null colon cancer cells as a model we demonstrated that p65BTK silencing or chemical inhibition overcame the 5‐fluorouracil resistance of CRC cell lines and patient‐derived organoids and significantly reduced the growth of xenografted tumours. Mechanistically, we show that blocking p65BTK in drug‐resistant cells abolished a 5‐FU‐elicited TGFB1 protective response and triggered E2F‐dependent apoptosis. Taken together, our data demonstrated that targeting p65BTK restores the apoptotic response to chemotherapy of drug‐resistant CRCs and gives a proof‐of‐concept for suggesting the use of BTK inhibitors in combination with 5‐FU as a novel therapeutic approach in CRC patients. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Published
2020
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44. VGF Involvement on Parkinson’s disease

Author

Cocco, Cristina, Angioni, Laura, Bongioanni, Paolo, Carta, Manolo, D’Amato, Filomena, Lisci, Carlo, and Ferri, Gian-Luca

Subjects
Parkinson’s disease, VGF peptides, substantia nigra
Abstract

Parkinson’s disease (PD) is a neurological disorder characterized by a progressive degeneration of dopaminergic neurons of the substantia nigra, that results in dopamine depletion in the striatum with a deregulation of a number of substances/ neurotrasmitters. A previous study demonstrated a clear-cut decrease of certain peptides derived from the VGF gene in the parietal cortex from patients affected by PD. Hence, we used an animal model of PD, and plasma samples from PD patients and control subjects to investigate the role of VGF in PD. Rats (Sprague Dawley) were treated with injections of 6 hydroxydopamine into the medial forebrain bundle, anesthetized and perfused with 4% paraformaldehyde 3 weeks (group 1: n= 8) or 6 weeks (group 2: n=17) after injection. In addition, subcutaneous injections of L-DOPA (6mg/ Kg) were carried out one week before the sacrifice in rats from group 1 and 2 (n=4 and n=6, respectively). We raised antibodies to the C-terminal end of the human VGF sequence, for immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA, IC50 = 15 pmol/ml). For pilot experiments, human plasma samples were taken from subjects affected by PD (n=13, age range: 51–84 years), and from age matched controls (n=10), to be used for ELISA experiments. We analysed the substantia nigra (SN) of the PD rats using VGF-C-terminus antiserum in double staining with antibodies to glutamate decarboxylase (GAD), tyrosine hydroxylase (TH), serotonin and Substance P. In the control side, VGF-C-terminus peptides were found in neurone terminals/axons within the pars compacta (SNC) and reticulata (SNR) of the substantia nigra, co-localised with GAD and substance P (but not with serotonin or TH) and in closed connection with TH cell bodies of the SNC. In the PD side, while the GAD staining remains unaffected, VGF-C-terminus and substance P immnureactivities disappeared (compared to the control side) in a large population of neurone terminals of both SNC and SNR, and in relation with the TH decrease. When L-DOPA was applied to the animals, VGF-C-terminus immunoreactivity on the PD side returned comparable to the control side. By ELISA, high concentrations of proVGF, in the order of 600-2000 pmol/ml were shown in the plasma from controls with a significant decrease in PD samples (p=0,0029). In conclusion, using the animal models, we showed here for the first time that VGF is involved into nigro-striatal circuits and down-regulated in PD. Besides, of a great interest, is the finding that a deregulation of VGF can occur also in humans., Italian Journal of Anatomy and Embryology, Vol 119, No 1 (Supplement) 2014

Published
2015
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45. VGF Protein and Its C-Terminal Derived Peptides in Amyotrophic Lateral Sclerosis: Human and Animal Model Studies

Author

Brancia, Carla, primary, Noli, Barbara, additional, Boido, Marina, additional, Boi, Andrea, additional, Puddu, Roberta, additional, Borghero, Giuseppe, additional, Marrosu, Francesco, additional, Bongioanni, Paolo, additional, Orrù, Sandro, additional, Manconi, Barbara, additional, D’Amato, Filomena, additional, Messana, Irene, additional, Vincenzoni, Federica, additional, Vercelli, Alessandro, additional, Ferri, Gian-Luca, additional, and Cocco, Cristina, additional

Published
2016
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46. Abstract B121: A novel oncogenic BTK isoform is overexpressed in colon cancers and required for RAS-mediated transformation

Author

Grassilli, Emanuela, primary, Pisano, Fabio, additional, Cialdella, Annamaria, additional, Bonomo, Sara, additional, Missaglia, Carola, additional, Cerrito, Maria Grazia, additional, Masiero, Laura, additional, Ianzano, Leonarda, additional, Narloch, Robert, additional, D'Amato, Filomena, additional, Noli, Barbara, additional, Ferri, Gian Luca, additional, Leone, Biagio E., additional, Stanta, Giorgio, additional, Bonin, Serena, additional, Helin, Kristian, additional, Giovannoni, Roberto, additional, and Lavitrano, Marialuisa, additional

Published
2015
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47. VGF Peptide Profiles in Type 2 Diabetic Patients’ Plasma and in Obese Mice

Author

D’Amato, Filomena, primary, Noli, Barbara, additional, Angioni, Laura, additional, Cossu, Efisio, additional, Incani, Michela, additional, Messana, Irene, additional, Manconi, Barbara, additional, Solinas, Paola, additional, Isola, Raffaella, additional, Mariotti, Stefano, additional, Ferri, Gian-Luca, additional, and Cocco, Cristina, additional

Published
2015
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50. Reduction of Total Brain and Cerebellum Volumes Associated With Neuronal Autoantibodies in Patients With APECED.

Author

Meloni, Antonella, Corda, Giulia, Saba, Luca, Ferri, Gian-Luca, Mariotti, Stefano, and Cocco, Cristina

Abstract

In autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED), autoantibodies (AutoAbs) labeling brain neurons were reported; conversely, brain MRI alterations associated with these AutoAbs were never reported.

Published
2019
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